CN105237650A - Method for extracting panax polysaccharide from american ginseng seed coats - Google Patents
Method for extracting panax polysaccharide from american ginseng seed coats Download PDFInfo
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- CN105237650A CN105237650A CN201510814771.9A CN201510814771A CN105237650A CN 105237650 A CN105237650 A CN 105237650A CN 201510814771 A CN201510814771 A CN 201510814771A CN 105237650 A CN105237650 A CN 105237650A
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- radix panacis
- panacis quinquefolii
- polysaccharide
- panax
- seed coat
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Abstract
The invention relates to a method for extracting panax polysaccharide from american ginseng seed coats, which solves the technical problems that existing methods for extracting the panax polysaccharide from the american ginseng seed coats are fussy in steps, long in extraction time and low in yield, and comprises steps: (1) american ginseng seed coats are stored into an extraction tank after being smashed, than are added with purified water, and are extracted by adopting ultrasonic waves in low temperature for 20-80min; (2) extractives which are obtained in the step (1) are filtered into a settling tank, alcohol is added into the settling tank of a filter liquid to obtain a mixing liquid which is 70%-90% in concentration of volume percent of the alcohol, is stood for 8-12 hours, and then obtains undissolved substances by being filtered, and the undissolved substances are dried to obtain the panax polysaccharide; and (3) panax polysaccharide powder is obtained from the panax polysaccharide by adopting a spraying dried method. The method for extracting the panax polysaccharide from the american ginseng seed coats can be used to extract the panax polysaccharide.
Description
Technical field
The present invention relates to a kind of extracting method of Radix Panacis Quinquefolii active substance, particularly a kind of method extracting Radix Panacis Quinquefolii polysaccharide from Radix Panacis Quinquefolii seed coat.
Background technology
Radix Panacis Quinquefolii is Araliaceae, and bitter is cool in nature, enter the heart, lung, kidney channel, function, can nourishing Yin and falling fire, supplementing QI for promoting the production of body fluid based on help, be clinically usually used in chronic cough of deficiency lung, lung wither, cough breathe heavily, spit blood, the various disease conditions such as consumption of YIN caused by febrile disease, thirsty and dry pharynx, Shao Jin, the fire of deficiency type are tired of tired, toothache due to stomach-fire.At present mainly saponin(e is concentrated on to the research of Radix Panacis Quinquefolii, less to other study of active components.
Relevant research shows, Radix Panacis Quinquefolii polysaccharide has immunomodulatory, antitumor, hypoglycemic, the effect such as radioprotective, prevention influenza and upper respiratory tract infection, has important research and development using value.
Seeds of American ginseng is very precious, and in order to meet the demand of Radix Panacis Quinquefolii plantation, seed has to pass through peeling, could store or utilize after separating separately.Research display, is rich in the plurality of active ingredients such as multiple ginsenoside, polysaccharide, volatile oil, amino acid in Radix Panacis Quinquefolii seed coat; Get son peeling, in the treating processes of seeds of American ginseng, often pericarp is directly abandoned as waste, cause the waste of resource.
At present, less about Radix Panacis Quinquefolii polysaccharide Study on extraction, the extracting method existing research of boiling water circumfluence method to Radix Panacis Quinquefolii polysaccharide in Radix Panacis Quinquefolii ginseng palpus waste residue such as Zhang Renquan, but screening is not optimized to polysaccharide extracting process in Radix Panacis Quinquefolii seed coat, and the method steps of existing extraction Radix Panacis Quinquefolii polysaccharide is loaded down with trivial details, extraction time is long, product yield is lower.
Summary of the invention
The present invention is exactly to solve the existing technical problem that extraction step is loaded down with trivial details, extraction time long and product yield is lower extracted Radix Panacis Quinquefolii polysaccharide and exist from Radix Panacis Quinquefolii seed coat, providing a kind of method extracting Radix Panacis Quinquefolii polysaccharide from Radix Panacis Quinquefolii seed coat that extraction step is simple, extraction time is short, product yield is high.
For this reason, the invention provides a kind of method extracting Radix Panacis Quinquefolii polysaccharide from Radix Panacis Quinquefolii seed coat, it comprises following steps: (1) loads in extractor after being pulverized by Radix Panacis Quinquefolii seed coat, then adds purified water, adopts ultrasonic low temperature to extract 20 ~ 80min; (2) extract is filtered in slurry tank, filtrate adds alcohol in slurry tank, and the concentration of volume percent obtaining alcohol is the mixed solution of 70%-90%, leaves standstill 8 ~ 12 hours, then filter and obtain insolubles, namely obtain Radix Panacis Quinquefolii polysaccharide by after insolubles drying; (3) spray drying process is adopted to obtain Radix Panacis Quinquefolii polysaccharide powder the Radix Panacis Quinquefolii polysaccharide obtained.
Preferably, in step (1), the mass ratio of purified water and Radix Panacis Quinquefolii seed coat is (1 ~ 10): 1.
Preferably, in step (1), the power of ultrasonic extraction is 70 ~ 200W.
Preferably, in step (3), spray-dired pressure is 100 ~ 120Mpa.
Ultrasonic technology is applied to the Study on extraction of Radix Panacis Quinquefolii seed coat polysaccharide by the present invention, by the optimal screening to extraction conditions, draw optimum extraction process parameter, can be the structural characterization of Radix Panacis Quinquefolii polysaccharide, active function is tested and the suitability for industrialized production of Radix Panacis Quinquefolii polysaccharide product lays the foundation.The present invention makes that extraction time is short, output yield is high, and extracts while the saponin(e achieved in Radix Panacis Quinquefolii seed coat and polysaccharide, further improves extraction efficiency.Step of the present invention is simple, overcomes the problem that in existing extracting method, extraction time is long, Extracting temperature is high.The present invention is that the extraction of bioactive ingredients in Radix Panacis Quinquefolii provides new approach and thinking.
Embodiment
Be described further below in conjunction with the method for embodiment to the Radix Panacis Quinquefolii polysaccharide in the present invention's ultrasonic extraction Radix Panacis Quinquefolii seed coat.
Embodiment 1
(1) load in extractor after Radix Panacis Quinquefolii seed coat being pulverized, then add 1 times amount purified water, adopt power to be extract 20min at ultrasonic wave 30 DEG C of temperature of 70w;
(2) be filtered in slurry tank by extract, filtrate adds alcohol in slurry tank, and the concentration of volume percent obtaining alcohol is the mixed solution of 70%, leaves standstill 8 hours, then filters and obtains insolubles;
(3) adopted by insolubles spray drying process to obtain Radix Panacis Quinquefolii polysaccharide powder, spray-dired pressure is 100Mpa, and yield is 3.5%, and the content of Radix Panacis Quinquefolii polysaccharide is 80%.
Embodiment 2
(1) load in extractor after Radix Panacis Quinquefolii seed coat being pulverized, then add 3 times amount purified water, adopt power to be extract 50min at ultrasonic wave 40 DEG C of temperature of 120w;
(2) be filtered in slurry tank by extract, filtrate adds alcohol in slurry tank, and the concentration of volume percent obtaining alcohol is the mixed solution of 80%, leaves standstill 10 hours, then filters and obtains insolubles;
(3) adopted by insolubles spray drying process to obtain Radix Panacis Quinquefolii polysaccharide powder, spray-dired pressure is 110Mpa, and yield is 3.8%, and the content of Radix Panacis Quinquefolii polysaccharide is 80%.
Embodiment 3
(1) load in extractor after Radix Panacis Quinquefolii seed coat being pulverized, then add 5 times amount purified water, adopt power to be extract 60min at ultrasonic wave 30 DEG C of temperature of 110w;
(2) be filtered in slurry tank by extract, filtrate adds alcohol in slurry tank, and the concentration of volume percent obtaining alcohol is the mixed solution of 80%, leaves standstill 8 hours, then filters and obtains insolubles;
(3) adopted by insolubles spray drying process to obtain Radix Panacis Quinquefolii polysaccharide powder, spray-dired pressure is 120Mpa, and yield is 3.6%, and the content of Radix Panacis Quinquefolii polysaccharide is 80%.
Embodiment 4
(1) load in extractor after Radix Panacis Quinquefolii seed coat being pulverized, then add 3 times amount purified water, adopt power to be extract 50min at ultrasonic wave 35 DEG C of temperature of 95w;
(2) be filtered in slurry tank by extract, filtrate adds alcohol in slurry tank, and the concentration of volume percent obtaining alcohol is the mixed solution of 90%, leaves standstill 10 hours, then filters and obtains insolubles;
(3) adopted by insolubles spray drying process to obtain Radix Panacis Quinquefolii polysaccharide powder, spray-dired pressure is 100Mpa, and yield is 3.2%, and the content of Radix Panacis Quinquefolii polysaccharide is 80%.
Embodiment 5
(1) load in extractor after Radix Panacis Quinquefolii seed coat being pulverized, then add 10 times amount purified water, adopt power to be extract 60min at ultrasonic wave 30 DEG C of temperature of 115w;
(2) be filtered in slurry tank by extract, filtrate adds alcohol in slurry tank, and the concentration of volume percent obtaining alcohol is the mixed solution of 85%, leaves standstill 12 hours, then filters and obtains insolubles;
(3) adopted by insolubles spray drying process to obtain Radix Panacis Quinquefolii polysaccharide powder, spray-dired pressure is 110Mpa, and yield is 3.8%, and the content of Radix Panacis Quinquefolii polysaccharide is 80%.
Embodiment 6
(1) load in extractor after Radix Panacis Quinquefolii seed coat being pulverized, then add 4 times amount purified water, adopt power to be extract 80min at ultrasonic wave 60 DEG C of temperature of 200w;
(2) be filtered in slurry tank by extract, filtrate adds alcohol in slurry tank, and the concentration of volume percent obtaining alcohol is the mixed solution of 75%, leaves standstill 12 hours, then filters and obtains insolubles;
(3) adopted by insolubles spray drying process to obtain Radix Panacis Quinquefolii polysaccharide powder, spray-dired pressure is 120Mpa, and yield is 4.0%, and the content of Radix Panacis Quinquefolii polysaccharide is 80%.
Claims (4)
1. from Radix Panacis Quinquefolii seed coat, extract a method for Radix Panacis Quinquefolii polysaccharide, it is characterized in that comprising following steps:
(1) load in extractor after Radix Panacis Quinquefolii seed coat being pulverized, then add purified water, adopt ultrasonic wave at 30 ~ 60 DEG C, extract 20 ~ 80min;
(2) extract that step (1) obtains is filtered in slurry tank, filtrate adds alcohol in slurry tank, the concentration of volume percent obtaining alcohol is the mixed solution of 70%-90%, leave standstill 8 ~ 12 hours, then filter and obtain insolubles, namely obtain Radix Panacis Quinquefolii polysaccharide by after insolubles drying;
(3) spray drying process is adopted to obtain Radix Panacis Quinquefolii polysaccharide powder the Radix Panacis Quinquefolii polysaccharide obtained.
2. the method extracting Radix Panacis Quinquefolii polysaccharide from Radix Panacis Quinquefolii seed coat according to claim 1, is characterized in that in described step (1), and the mass ratio of purified water and Radix Panacis Quinquefolii seed coat is (1 ~ 10): 1.
3. the method extracting Radix Panacis Quinquefolii polysaccharide from Radix Panacis Quinquefolii seed coat according to claim 1, is characterized in that in described step (1), and the power of ultrasonic extraction is 70 ~ 200W.
4. the method extracting Radix Panacis Quinquefolii polysaccharide from Radix Panacis Quinquefolii seed coat according to claim 1, is characterized in that, in described step (3), spray-dired pressure is 100 ~ 120Mpa.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510814771.9A CN105237650A (en) | 2015-11-20 | 2015-11-20 | Method for extracting panax polysaccharide from american ginseng seed coats |
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| CN201510814771.9A CN105237650A (en) | 2015-11-20 | 2015-11-20 | Method for extracting panax polysaccharide from american ginseng seed coats |
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| CN201510814771.9A Pending CN105237650A (en) | 2015-11-20 | 2015-11-20 | Method for extracting panax polysaccharide from american ginseng seed coats |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106397625A (en) * | 2016-10-28 | 2017-02-15 | 宁波中药制药股份有限公司 | Method for extracting panax quinquefolius polysaccharides from panax quinquefolius fibrous roots |
| CN115710319A (en) * | 2022-11-04 | 2023-02-24 | 山东颐阳生物科技股份有限公司 | Settling tank and settling method for extracting American ginseng polysaccharide based on American ginseng seed coats |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104095872A (en) * | 2014-07-23 | 2014-10-15 | 中国农业科学院作物科学研究所 | American ginseng polysaccharide with anti-inflammation enhancement function as well as preparation method and application thereof |
-
2015
- 2015-11-20 CN CN201510814771.9A patent/CN105237650A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104095872A (en) * | 2014-07-23 | 2014-10-15 | 中国农业科学院作物科学研究所 | American ginseng polysaccharide with anti-inflammation enhancement function as well as preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 刘婷等: "超声波法提取西洋参多糖的工艺研究", 《西北林学院学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106397625A (en) * | 2016-10-28 | 2017-02-15 | 宁波中药制药股份有限公司 | Method for extracting panax quinquefolius polysaccharides from panax quinquefolius fibrous roots |
| CN106397625B (en) * | 2016-10-28 | 2019-09-20 | 宁波中药制药股份有限公司 | A method of extracting Radix Panacis Quinquefolii polysaccharide from American Ginseng fibrous root |
| CN115710319A (en) * | 2022-11-04 | 2023-02-24 | 山东颐阳生物科技股份有限公司 | Settling tank and settling method for extracting American ginseng polysaccharide based on American ginseng seed coats |
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