CN104546657B - Method for extracting cosmetics used active matters from cordyceps sinensis - Google Patents
Method for extracting cosmetics used active matters from cordyceps sinensis Download PDFInfo
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- 241001248610 Ophiocordyceps sinensis Species 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000002537 cosmetic Substances 0.000 title claims abstract description 18
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 58
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000000284 extract Substances 0.000 claims abstract description 25
- 239000000843 powder Substances 0.000 claims abstract description 19
- 238000000605 extraction Methods 0.000 claims abstract description 18
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 239000012528 membrane Substances 0.000 claims abstract description 8
- 235000011187 glycerol Nutrition 0.000 claims description 27
- 239000000706 filtrate Substances 0.000 claims description 16
- 241000190633 Cordyceps Species 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 13
- 241000233866 Fungi Species 0.000 claims description 7
- 240000001307 Myosotis scorpioides Species 0.000 claims description 7
- 239000008346 aqueous phase Substances 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- 238000005119 centrifugation Methods 0.000 claims description 6
- 241000289667 Erinaceus Species 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 abstract description 7
- 239000000203 mixture Substances 0.000 abstract description 5
- 238000010438 heat treatment Methods 0.000 abstract description 4
- 230000006866 deterioration Effects 0.000 abstract 1
- 238000001471 micro-filtration Methods 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
- 229920005862 polyol Polymers 0.000 abstract 1
- 150000003077 polyols Chemical class 0.000 abstract 1
- 230000001235 sensitizing effect Effects 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
- 239000007788 liquid Substances 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical group CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229940083957 1,2-butanediol Drugs 0.000 description 3
- 241001018099 Alloteropsis Species 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 3
- 235000017898 Digitaria ciliaris Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- BMRWNKZVCUKKSR-UHFFFAOYSA-N butane-1,2-diol Chemical compound CCC(O)CO BMRWNKZVCUKKSR-UHFFFAOYSA-N 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- WCVRQHFDJLLWFE-UHFFFAOYSA-N pentane-1,2-diol Chemical compound CCCC(O)CO WCVRQHFDJLLWFE-UHFFFAOYSA-N 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- AXPZIVKEZRHGAS-UHFFFAOYSA-N 3-benzyl-5-[(2-nitrophenoxy)methyl]oxolan-2-one Chemical compound [O-][N+](=O)C1=CC=CC=C1OCC1OC(=O)C(CC=2C=CC=CC=2)C1 AXPZIVKEZRHGAS-UHFFFAOYSA-N 0.000 description 2
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 206010062717 Increased upper airway secretion Diseases 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 229960005305 adenosine Drugs 0.000 description 2
- 235000019437 butane-1,3-diol Nutrition 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 208000026435 phlegm Diseases 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 206010013911 Dysgeusia Diseases 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 210000003056 antler Anatomy 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 208000013116 chronic cough Diseases 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 208000012059 diaphragm disease Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 210000000629 knee joint Anatomy 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 235000015961 tonic Nutrition 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 229960000716 tonics Drugs 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a method for extracting cosmetics used active matters from cordyceps sinensis, wherein the method is further suitable for extracting active matters from cordyceps sinensis mycelia. The method comprises the following steps: adding pulverized cordyceps sinensis or cordyceps sinensis mycelia and water into an extraction device, heating through water bath, and extracting for the first time; extracting a powder for the second time by using the mixture of glycerol and water; concentrating the obtained extracts in vacuum rotationally, centrifuging and filtering with a microfiltration membrane in sequence; and adding an appropriate amount of a polyol to obtain a final extract. The extract obtained by adopting the method is free from sensitizing organic solvent residue, is improved in the aspects of deterioration and precipitation during storage and is improved in the compatibility with the formula of the cosmetics.
Description
Technical field
The present invention relates to a kind of field of traditional Chinese medicine extraction, refer in particular to a kind of for the Cordyceps sinensis active matter in cosmetics or winter
The extracting method of worm summer grass mycelia active thing.
Background technology
Cordyceps sinensis is a kind of rare traditional Chinese medicine of China, is classified as Chinese three big tonics together with ginseng, pilose antler.Early in one
The Seventh Five-Year Plan 7 years《The new compilation of materia medica》In just have " Cordyceps sinensis Gan Pingbao lung, kidney-nourishing, mend marrow, hemostasis and phlegm, phthisical cough, control diaphragm disease
All good " record.The traditional Chinese medical science thinks, Chinese caterpillar fungus enters lung kidney two warp, can tonifying lung cloudy, and can kidney-replenishing, cure mainly kidney deficiency, impotence and seminal emission,
Soreness of waist and knee joint, eak after being ill, chronic cough is weak, phthisical cough phlegm blood, and spontaneous sweating etc., is that uniquely one kind can balance simultaneously, adjust the moon
The Chinese medicine of sun.And cordyceps mycelia is the stroma of Cordyceps sinensis, there is the composition similar to Cordyceps sinensis, and the containing of active matter
Amount is very high.At present, Cordyceps sinensis and its mycelium are mainly used in health care, food aspect, in the fortune of the beauty industry such as cosmetics
With little, but their active component Cordyceps sinensis polysaccharide, cordycepic acid, superoxide dismutase and amino acid of being rich in etc. have well
Cosmetic result.
Considerably less with regard to the report of Cordyceps sinensis and the extracting method of cordyceps mycelia active material, through the present invention at present
The long-term experiment of people finds, prior art all has some shortcomings.
Patent publication No. CN 101928352A obtains single having by steps such as water backflow, concentration, filtration, column chromatographies
Effect composition.For cosmetic field, the composition such as amino acid in cordyceps mycelia, polysaccharide, adenosine and cordycepic acid all has U.S.
Hold effect of skin care, the cordyceps mycelia in the method is not comprehensively utilized.
Number of patent application CN 93112049.7 disclose with ethanol or acetone soak 7-10 days, filtration, reduced pressure concentration side
The method that method prepares the extract of Cordceps militaris, this process takes oversize, is unfavorable for industrialized production.
Number of patent application CN 200710030010.X and CN 201110004151.0 discloses by heating acidolysis, pulverizing
Ultrasonic, the step such as filter, concentrate and prepare the activity extract of cordyceps mycelia, but there is the irritant gas of extract band in the method
After taste and Ultrasonic Pulverization, extract easily separates out the problem of particle precipitation in storing process.
Number of patent application CN 201310303417.0 discloses the technique by adding solvent heating extraction, filter, drying
Prepare cordyceps mycelia extract.Application number CN 201010017125.7, application number CN 201010017125.7 China specially
Profit discloses prepares cordyceps mycelia freeze-dried powder by ultrasonic extraction, thickening filtration, freeze-drying.The extraction of these methods preparation
Thing is pressed powder, and with liquid extraction liquid phase ratio, being added into cosmetics has insoluble granule, the especially cosmetics of water aqua type,
Also easily separate out and form precipitation.
Content of the invention
It is an object of the invention to provide a kind of extracting method of Cordyceps sinensis active matter used for cosmetic, also it is simultaneously suitable for the winter
The extraction of worm summer grass fermentation mycelium, this method is simple and reliable, is avoided that relevant material separates out and forms precipitation, improves such
The quality of cosmetics.The extract that the method obtains is applied to the cosmetics of the formulations such as cream, emulsion, aqua, especially suitable
Cosmetics in water aqua type.
For reaching above-mentioned purpose, the invention provides a kind of preparation method of cordyceps extracting liquid used for cosmetic, comprise
Following steps：
(1) Cordyceps sinensis or cordyceps mycelia powder and water is 1 in mass ratio:20～30 ratio mixing, 20～
In 100 DEG C of water-baths, constant temperature stirring extraction was filtered after 30～100 minutes；
(2) after filtering, solid adds the glycerine of same step (1) aqueous phase homogenous quantities to be extracted with the mixed liquor of water, 20～
In 100 DEG C of water-baths, constant temperature stirring extraction was filtered after 30～100 minutes, merged the body of the filtrate extracted twice, wherein glycerine and water
Long-pending ratio is 1:20～50；
(3) by after through being concentrated in vacuo to Chinese caterpillar fungus or erinaceus mycelium powder quality 10～50 times of the filtrate of step (2) gained, from
Filter after the heart, by filtrate and glycerine in mass ratio 1:0.5～3 mixes, and final extract is obtained.
In some embodiments, the granularity of cordyceps mycelia powder is 40～100 mesh.
In some embodiments, the filtration in step (3) is to be processed using 0.22um filter membrane.
In some embodiments, the rotating speed of centrifugation is 6000～12000r/min.
Main advantages of the present invention are：
Amino acid is extracted from Chinese caterpillar fungus or its mycelium by this method, polysaccharide, adenosine mainly become with Chinese caterpillar funguses such as cordycepic acids
Comprehensive recovery rate is divided to maintain higher level.In addition the method being provided using the present invention, not only increase in extract Chinese caterpillar fungus or
The solubility of each active matter of its mycelium, and solve Cordyceps sinensis and its mycelia extract in refrigeration, heating process
Separate out the FAQs of precipitation because activity concentration is too high, and in 4 DEG C, 25 DEG C of room temperature, 45 DEG C and subzero 18 DEG C of constant temperature shapes
Can stablize more than 6 months under state, solution keeps clear, no precipitation and macroscopic foreign matter separate out, with cosmetics its
The compatibility of his raw material is good, adds as raw material in suitable cosmetics.
Specific embodiment
Described below is the preferred embodiment of the present invention, and what the present invention was protected is not limited to the following side of being preferable to carry out
Formula.It should be pointed out that for a person skilled in the art on the basis of this innovation and creation design, some deformation of making and
Improve, broadly fall into protection scope of the present invention.
In the embodiment of the present invention, Cordyceps sinensis is obtained from commercially available.Mycelium is to be fermented by hair spore (Cs-C-Q80) by China
Obtain with artificial culture.Realized by following condition in the embodiment of the present invention：
Experiment condition：After Cordyceps sinensis is extracted with water after pulverizing, residue alcohol and water mixed liquor carries out second extraction, extracts
Liquid is through concentrating, centrifugation, filter after, by extract and the polyalcohol such as glycerine, 1,3-BDO, 1,2-PD or ethanol, second
The mixing such as acid (adopts same material) in each embodiment, be statically placed in the environment of 45 DEG C, 4 DEG C, subzero 18 DEG C and 25 DEG C of room temperature
Under, under the corresponding time period, whether the smell of observation extract, color change, pH value change and have the aspects such as material precipitation
Judge the stability of extract.
Residue after extraction obtains its quality using freeze-drying, Cordyceps sinensis before extraction after quality difference value and winter used
The ratio of worm summer grass original quality is worth to the recovery rate of extract.
Embodiment 1
(1) weigh 20.0g Cordyceps sinensis is dried and be crushed to 40 mesh, powder and water are 1 in mass ratio:20 ratio mixing,
In 20 DEG C of water-baths, constant temperature stirring is filtered after extracting 30 minutes；
(2) after filtering, solid adds the glycerine of same step (1) aqueous phase homogenous quantities and the mixed liquor of water, permanent in 20 DEG C of water-baths
Temperature stirring is filtered after extracting 30 clocks, merges the filtrate extracted twice, wherein glycerine and the volume ratio of water are 1:20；
(3) filter vacuum of (2) gained is concentrated into 10 times of Cordyceps sinensis powder quality, then through 6000r/min centrifugation
Cross 0.22um filter membrane after 20 minutes to process, filtrate and glycerine are with 1:0.5 mass ratio mixing, obtains final Cordyceps sinensis and extracts
Liquid.The effective component extraction rate of this embodiment is 83.61%, and extract is carried out stability experiment.Respectively use 1,2- butanediol,
1,3-BDO, 1,2- pentanediol replaces glycerine to carry out testing as a comparison, and it the results are shown in Table 1.
Table 1 stability experiment result
Embodiment 2
(1) weigh 20.0g Cordyceps sinensis is dried and be crushed to 100 mesh, powder and water are 1 in mass ratio:30 ratio mixing,
In 100 DEG C of water-baths, constant temperature stirring is filtered after extracting 100 minutes；
(2) after filtering, solid adds the glycerine of same step (1) aqueous phase homogenous quantities and the mixed liquor of water, in 100 DEG C of water-baths
Constant temperature stirring is filtered after extracting 100 clocks, merges the filtrate extracted twice, wherein glycerine and the volume ratio of water are 1:50；
(3) filter vacuum of (2) gained is concentrated into 50 times of Cordyceps sinensis powder quality, then through 12000r/min centrifugation
Cross 0.22um filter membrane after 20 minutes to process, filtrate and glycerine are with 1:3 mass ratio mixing, obtains final cordyceps extracting liquid.
The effective component extraction rate of this embodiment is 90.32%, and extract is carried out stability experiment.Respectively use 1,2- butanediol, 1,
3- butanediol, 1,2- pentanediol replaces glycerine to be tested, and as a comparison, it the results are shown in Table 2.
Table 2 stability experiment result
Embodiment 3
(1) weigh 20.0g Cordyceps sinensis is dried and be crushed to 60 mesh, powder and water are 1 in mass ratio:25 ratio mixing,
In 80 DEG C of water-baths, constant temperature stirring is filtered after extracting 80 minutes；
(2) after filtering, solid adds the glycerine of same step (1) aqueous phase homogenous quantities and the mixed liquor of water, permanent in 80 DEG C of water-baths
Temperature stirring is filtered after extracting 80 clocks, merges the filtrate extracted twice, wherein glycerine and the volume ratio of water are 1:30；
(3) filter vacuum of (2) gained is concentrated into 30 times of Cordyceps sinensis powder quality, then through 8000r/min centrifugation
Cross 0.22um filter membrane after 20 minutes to process, filtrate and glycerine are with 1:1 mass ratio mixing, obtains final cordyceps extracting liquid.
The effective component extraction rate of this embodiment is 92.08%, and extract is carried out stability experiment.Respectively use 1,2- butanediol, 1,
3- butanediol, 1,2- pentanediol replaces glycerine to be tested, and as a comparison, it the results are shown in Table 3.
Table 3 stability experiment result
Embodiment 4
(1) weigh 20.0g Cordyceps sinensis is dried and be crushed to 80 mesh, powder and water are 1 in mass ratio:28 ratio mixing,
In 60 DEG C of water-baths, constant temperature stirring is filtered after extracting 60 minutes；
(2) after filtering, solid adds the glycerine of same step (1) aqueous phase homogenous quantities and the mixed liquor of water, permanent in 60 DEG C of water-baths
Temperature stirring is filtered after extracting 60 clocks, merges the filtrate extracted twice, wherein glycerine and the volume ratio of water are 1:40；
(3) filter vacuum of (2) gained is concentrated into 40 times of Cordyceps sinensis quality, then is centrifuged 20 points through 10000r/min
Cross 0.22um filter membrane after clock to process, filtrate and glycerine are with 1:2 mass ratio mixing, obtains final cordyceps extracting liquid.This is real
The effective component extraction rate applying example is 89.66%, and extract is carried out stability experiment.Replaced with ethanol, acetone, acetic acid respectively
Glycerine is tested, and as a comparison, it the results are shown in Table 4.
Table 4 stability experiment result
Embodiment 5
(1) weigh 20.0g cordyceps sinensis mycelium powder is dried and be broken to 50 mesh, powder and water are 1 in mass ratio:22 ratio
Mixing, constant temperature stirring in 50 DEG C of water-baths is filtered after extracting 50 minutes；
(2) after filtering, solid adds the glycerine of same step (1) aqueous phase homogenous quantities and the mixed liquor of water, permanent in 50 DEG C of water-baths
Temperature stirring is filtered after extracting 50 clocks, merges the filtrate extracted twice, wherein glycerine and the volume ratio of water are 1:25；
(3) filter vacuum of (2) gained is concentrated into 25 times of cordyceps sinensis mycelium powder end quality, then through 8000r/
Min crosses 0.22um filter membrane after being centrifuged 20 minutes and processes, and filtrate and glycerine are with 1:1.5 mass ratio mixing, obtains the final worm summer in winter
Careless mycelia extract.The effective component extraction rate of this embodiment is 91.58%, and extract is carried out stability experiment.Respectively
Glycerine is replaced to be tested with ethanol, acetone, acetic acid, it the results are shown in Table 5 as a comparison.
Table 5 stability experiment result
Claims (3)
1. a kind of extracting method of Cordyceps sinensis active matter used for cosmetic is it is characterised in that comprise the steps of：
(1) Cordyceps sinensis or cordyceps mycelia powder and water is 1 in mass ratio:20～30 ratio mixing, at 20～100 DEG C
In water-bath, constant temperature stirring extraction was filtered after 30～100 minutes；
(2) after filtering, solid adds the glycerine of same step (1) aqueous phase homogenous quantities to be extracted with the mixed liquor of water, 20～100
In DEG C water-bath, constant temperature stirring extraction was filtered after 30～100 minutes, merged the filtrate extracted twice, wherein the volume ratio of glycerine and water
For 1:20～50；
(3) by after through being concentrated in vacuo to Chinese caterpillar fungus or erinaceus mycelium powder quality 10～50 times of the filtrate of (2) gained, mistake after centrifugation
Filter, by filtrate and glycerine in mass ratio 1:0.5～3 mixes, and final extract is obtained.
2. extracting method according to claim 1 it is characterised in that in step (1) Chinese caterpillar fungus or erinaceus mycelium powder granularity
For 40～100 mesh.
3. extracting method according to claim 1 it is characterised in that step (3) in filter be using 0.22um filter membrane at
Reason.
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| CN201410740160.XA CN104546657B (en) | 2014-07-08 | 2014-12-05 | Method for extracting cosmetics used active matters from cordyceps sinensis |
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| CN2014103231474 | 2014-07-08 | ||
| CN201410740160.XA CN104546657B (en) | 2014-07-08 | 2014-12-05 | Method for extracting cosmetics used active matters from cordyceps sinensis |
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| TWI744668B (en) * | 2019-07-19 | 2021-11-01 | 克一股份有限公司 | Production method and application of rice protein extract extracted from cordyceps militaris ferment |
| CN113133951B (en) * | 2020-01-19 | 2023-06-09 | 克一股份有限公司 | Production method for extracting rice protein extract from cordyceps militaris fermentation product and application of rice protein extract |
| CN112656718A (en) * | 2021-02-03 | 2021-04-16 | 现代百朗德生物科技(江苏)有限公司 | Whitening mask containing cordyceps sinensis extract |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1189532A (en) * | 1997-01-25 | 1998-08-05 | 杨国祥 | Prodn. tech. of natural plant perfume for cigarette |
| JP2003292429A (en) * | 2002-04-01 | 2003-10-15 | Noevir Co Ltd | Skin care preparation |
| CN102641235A (en) * | 2012-05-18 | 2012-08-22 | 通德集团有限公司 | Rhodiola crenulata essence with skin softening and anti-wrinkle effects |
-
2014
- 2014-12-05 CN CN201410740160.XA patent/CN104546657B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1189532A (en) * | 1997-01-25 | 1998-08-05 | 杨国祥 | Prodn. tech. of natural plant perfume for cigarette |
| JP2003292429A (en) * | 2002-04-01 | 2003-10-15 | Noevir Co Ltd | Skin care preparation |
| CN102641235A (en) * | 2012-05-18 | 2012-08-22 | 通德集团有限公司 | Rhodiola crenulata essence with skin softening and anti-wrinkle effects |
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Denomination of invention: Method for extracting cosmetics used active matters from cordyceps sinensis Effective date of registration: 20171107 Granted publication date: 20170222 Pledgee: Bank of Bohai, Limited by Share Ltd, Yichang branch|Bank of China, Limited by Share Ltd, Three Gorges Branch|Bank of Hubei Limited by Share Ltd Yichang Nanhu sub branch|Bank of Hankou, Limited by Share Ltd, Yichang branch Pledgor: Dongyangguang Pharmaceutical Co., Ltd., Guangdong Registration number: 2017420000037 |
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Denomination of invention: A method for extracting active substance of Cordyceps sinensis for cosmetics Effective date of registration: 20210630 Granted publication date: 20170222 Pledgee: Bank of Bohai Limited by Share Ltd. Yichang branch|Bank of China Limited by Share Ltd. Three Gorges Branch|Bank of Hubei Limited by Share Ltd. Yichang Nanhu sub branch|Bank of Hankou Limited by Share Ltd. Yichang branch Pledgor: YICHANG SHANCHENGSHUIDU CORDYCEPS Co.,Ltd. Registration number: Y2021420000062 |
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