CN112656718A - Whitening mask containing cordyceps sinensis extract - Google Patents
Whitening mask containing cordyceps sinensis extract Download PDFInfo
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- CN112656718A CN112656718A CN202110147619.5A CN202110147619A CN112656718A CN 112656718 A CN112656718 A CN 112656718A CN 202110147619 A CN202110147619 A CN 202110147619A CN 112656718 A CN112656718 A CN 112656718A
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- 241001248610 Ophiocordyceps sinensis Species 0.000 title claims abstract description 81
- 230000002087 whitening effect Effects 0.000 title claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 14
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 239000011550 stock solution Substances 0.000 claims abstract description 12
- 239000007864 aqueous solution Substances 0.000 claims abstract description 6
- 238000007865 diluting Methods 0.000 claims abstract description 6
- 239000000835 fiber Substances 0.000 claims abstract description 6
- 238000010438 heat treatment Methods 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 230000001815 facial effect Effects 0.000 claims description 25
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 15
- TXFPEBPIARQUIG-UHFFFAOYSA-N 4'-hydroxyacetophenone Chemical compound CC(=O)C1=CC=C(O)C=C1 TXFPEBPIARQUIG-UHFFFAOYSA-N 0.000 claims description 10
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims description 10
- 239000000047 product Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 6
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- 229940015975 1,2-hexanediol Drugs 0.000 claims description 5
- ANZUDYZHSVGBRF-UHFFFAOYSA-N 3-ethylnonane-1,2,3-triol Chemical compound CCCCCCC(O)(CC)C(O)CO ANZUDYZHSVGBRF-UHFFFAOYSA-N 0.000 claims description 5
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- 235000004866 D-panthenol Nutrition 0.000 claims description 5
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- 229920000663 Hydroxyethyl cellulose Polymers 0.000 claims description 5
- 239000004354 Hydroxyethyl cellulose Substances 0.000 claims description 5
- 244000042430 Rhodiola rosea Species 0.000 claims description 5
- 235000003713 Rhodiola rosea Nutrition 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 5
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 5
- 229920000392 Zymosan Polymers 0.000 claims description 5
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 claims description 5
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 5
- 229940085237 carbomer-980 Drugs 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 229960003949 dexpanthenol Drugs 0.000 claims description 5
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 229960003180 glutathione Drugs 0.000 claims description 5
- FHKSXSQHXQEMOK-UHFFFAOYSA-N hexane-1,2-diol Chemical compound CCCCC(O)CO FHKSXSQHXQEMOK-UHFFFAOYSA-N 0.000 claims description 5
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- 229910052710 silicon Inorganic materials 0.000 claims description 5
- 239000010703 silicon Substances 0.000 claims description 5
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- 229960000281 trometamol Drugs 0.000 claims description 5
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 2
- 230000002421 anti-septic effect Effects 0.000 claims description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 2
- 239000003906 humectant Substances 0.000 claims description 2
- 229920002674 hyaluronan Polymers 0.000 claims description 2
- 229960003160 hyaluronic acid Drugs 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 19
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- 239000002537 cosmetic Substances 0.000 abstract description 10
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- 238000002835 absorbance Methods 0.000 description 15
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- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 12
- 230000005764 inhibitory process Effects 0.000 description 10
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- 230000002401 inhibitory effect Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
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- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 3
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- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 3
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
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- 241000191963 Staphylococcus epidermidis Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 150000003838 adenosines Chemical class 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
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- 229940035893 uracil Drugs 0.000 description 1
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a whitening mask containing a cordyceps sinensis extract, which comprises the cordyceps sinensis extract, a mask solution and a biological fiber mask, and a cordyceps sinensis extraction method comprises the following steps: step 1: selecting a cordyceps sinensis raw material, crushing the cordyceps sinensis raw material, adding 4-6% of 30% BG aqueous solution, heating to 70 ℃, then carrying out water bath for 3-5 hours, and filtering by using a 1-micron filter paper plate to obtain cordyceps sinensis stock solution; step 2: adding 4-6% BG into the stock solution obtained in the step 1, diluting the mixture with water until the mixture is colorless and transparent, and performing the step 3: and (3) filtering the liquid obtained in the step (2) by using a 0.22-micron filter paper plate to obtain the cordyceps sinensis extract. The invention uses the high-temperature water bath technology to strengthen the extraction and separation process, can effectively improve the extraction and separation rate, shorten the extraction time, save the cost, and even can improve the quality and the yield of products, and when the cosmetics contain a small amount of cordyceps sinensis extract, the cosmetics have obvious whitening and anti-aging effects.
Description
Technical Field
The invention relates to the technical field of facial mask liquid, and particularly relates to a whitening facial mask containing a cordyceps sinensis extract.
Background
Cordyceps sinensis, also known as Cordyceps sinensis, is a complex of stroma and larva corpse of Cordyceps sinensis, which is a fungus of Clavipitaceae, parasitizing on larva of insect of Lagotidae, and mainly grows in Sichuan, Yunnan, Gansu, Tibet and Qinghai. The Chinese pharmacopoeia records that the cordyceps sinensis contains alkaloid and has the bacteriostatic action on staphylococcus epidermidis and staphylococcus aureus. Has antiaging, antioxidant, and skin whitening effects. The cordyceps sinensis contains various active ingredients such as cordycepin, adenine, nucleoside, uracil and the like, the cordycepin is a derivative of adenosine, the pharmacological effect is obvious, and the content of the cordycepin is the basis of the quality of the cordyceps sinensis. Meanwhile, the cordyceps sinensis has a good effect of promoting penetration, and provides a wide prospect for further application and development of the cordyceps sinensis in facial mask liquid.
Disclosure of Invention
The invention aims to provide a whitening mask containing a cordyceps sinensis extract.
The technical scheme adopted by the invention is as follows:
a whitening mask containing a cordyceps sinensis extract is characterized in that: the facial mask comprises a cordyceps sinensis extract, a facial mask liquid and a biological fiber facial mask, wherein the mass percentage of the cordyceps sinensis extract is 0.05-0.1%, and the balance is the facial mask liquid, and the facial mask liquid is composed of the following raw materials in percentage by weight: 89.96-90% of yeast fermentation product filtrate, 0.02-0.04% of zymosan, 0.1-0.14% of carbomer 980, 0.08-0.12% of hydroxyethyl cellulose, 3-5% of glycerol, 0.2-0.4% of DPG, 0.1-0.3% of D-panthenol, 0.05-0.15% of trehalose, 0.4-0.6% of nicotinamide, 0.05-0.1% of rhodiola rosea extract, 1.0-1.2% of tromethamine, 0.01-0.09% of 140 ten thousand molecular weight HA, 1.5-2.5% of BG, 0.3-0.5% of p-hydroxyacetophenone, 0.3-0.7% of 1, 2-hexanediol, 0.04-0.06% of ethylhexyl glycerol, 0.1-0.3% of silicon wax, 0.01-0.03% of glutathione and the balance of deionized water.
The facial mask liquid contains at least one of humectant, antiseptic, aromatic and hyaluronic acid.
The method for extracting the cordyceps sinensis comprises the following steps:
step 1: selecting a cordyceps sinensis raw material, crushing the cordyceps sinensis raw material, adding 4-6% of 30% BG aqueous solution, heating to 70 ℃, then carrying out water bath for 3-5 hours, and filtering by using a 1-micron filter paper plate to obtain cordyceps sinensis stock solution;
step 2: adding 4-6% BG into the stock solution obtained in the step 1, and diluting the mixture with water until the mixture is colorless and transparent
And step 3: and (3) filtering the liquid obtained in the step (2) by using a 0.22-micron filter paper plate to obtain the cordyceps sinensis extract.
The invention has the advantages that: the extraction and separation process is enhanced by using a high-temperature water bath technology, the extraction and separation rate can be effectively improved, the extraction time is shortened, the cost is saved, the quality and the yield of products can be even improved, and when a small amount of cordyceps sinensis extract is contained in cosmetics, the cosmetics have obvious whitening and anti-aging effects.
Drawings
FIG. 1 is a graph showing the results of the inhibition of tyrosinase activity by the Cordyceps sinensis extract of the present invention.
Detailed Description
The present invention will be described in further detail with reference to fig. 1 and the following detailed description.
Example 1
A whitening mask containing a cordyceps sinensis extract comprises the cordyceps sinensis extract, a mask solution and a biological fiber mask, wherein the mass percent of the cordyceps sinensis extract is 0.05%, and the balance is the mask solution, and the mask solution comprises the following components in percentage by mass: 89.96% of yeast fermentation product filtrate, 0.02% of zymosan, 0.1% of carbomer 980, 0.08% of hydroxyethyl cellulose, 3% of glycerol, 0.2% of DPG, 0.1% of D-panthenol, 0.05% of trehalose, 0.4% of nicotinamide, 0.05% of rhodiola rosea extract, 1.0% of tromethamine, 0.01% of 140 ten thousand molecular weight of HA, 1.5% of BG, 0.3% of p-hydroxyacetophenone, 0.3% of 1, 2-hexanediol, 0.04% of ethylhexyl glycerol, 0.1% of water-soluble silicon wax, 0.01% of glutathione and deionized water for complement.
The extraction method of the cordyceps sinensis comprises the following steps:
step 1: selecting a cordyceps sinensis raw material, crushing the cordyceps sinensis raw material, adding a 4% 30% BG aqueous solution, heating to 70 ℃, carrying out water bath for 3 hours, and filtering by using a 1-micron filter paper plate to obtain a cordyceps sinensis stock solution;
step 2: taking the stock solution in the step 1, adding 4 percent BG, and diluting with water to be colorless and transparent
And step 3: and (3) filtering the liquid obtained in the step (2) by using a 0.22-micron filter paper plate to obtain the cordyceps sinensis extract.
The invention uses the high-temperature water bath technology to strengthen the extraction and separation process, can effectively improve the extraction and separation rate, shorten the extraction time, save the cost, and even can improve the quality and the yield of products, and when the cosmetics contain a small amount of cordyceps sinensis extract, the cosmetics have obvious whitening and anti-aging effects.
The tyrosinase activity inhibition assay of cordyceps sinensis facial mask fluid in example 1:
the purpose is as follows: and (5) measuring the half inhibition concentration of the cordyceps sinensis mask on the tyrosinase activity.
Materials: cordyceps sinensis mask
Sample preparation:
buffer solution: k2HPO4（8.71g/500mL）+KH2PO4（6.805g/500mL）→pH=6.80 （±0.02）
Substrate: about 0.3mg/mL of L-tyrosine
A spectrophotometer: uv, 480nm
The method comprises the following steps:
(1) enzyme concentration selection:
taking a small amount of enzyme, dissolving the enzyme by using a buffer solution, wherein the color is brown; adding 500. mu.L of (L-tyrosine (0.3 mg/mL)), 500. mu.L of buffer solution, 450. mu.L of pure water and 50. mu.L of enzyme into a 1.5mL centrifuge tube; before adding enzyme, water bath is carried out for 20min at 37 ℃; after the enzyme was added, the absorbance was measured at a wavelength of 480nm in a spectrophotometer at 37 ℃ in a water bath (enzyme addition-test) for 10 min. The enzyme concentration was adjusted to give an Absorbance (ABS) of between 0.45 and 0.55. The enzyme solution at this concentration was used as an experimental enzyme solution.
(2) Experimental addition
(3) Measuring absorbance at 480nm, making table, and plotting to obtain IC50。
Calculating the formula: inhibition (%) = [1- (S-SB)/(C-CB) ] + 100
Note that: before adding enzyme, water bath is carried out at 37 ℃ for 20 min; after adding enzyme, water bath at 37 ℃ (adding enzyme-testing time) for 10min for light splitting
The absorbance was measured at a wavelength of 480 nm. Water bath at 37 deg.C for 20min without enzyme.
Results
The results of the cordyceps sinensis mask on inhibiting the tyrosinase activity are shown in the table:
S：Sample SB：Sample Blank C：Control CB：Control Blank
and (4) conclusion: the Cordyceps facial mask has tyrosinase activity inhibiting effect, and IC50 value is about 26 μ L/mL.
Example 2
A whitening mask containing a cordyceps sinensis extract is characterized in that: the facial mask comprises a cordyceps sinensis extract, a facial mask solution and a biological fiber facial mask, wherein the mass percent of the cordyceps sinensis extract is 0.06%, and the balance is the facial mask solution, and the facial mask solution comprises the following components in percentage by mass: 89.98% of yeast fermentation product filtrate, 0.03% of zymosan, 0.12% of carbomer 980, 0.1% of hydroxyethyl cellulose, 4% of glycerol, 0.3% of DPG, 0.2% of D-panthenol, 0.1% of trehalose, 0.5% of nicotinamide, 0.07% of rhodiola rosea extract, 1.1% of tromethamine, 0.05% of 140 ten thousand molecular weight HA, 2% of BG, 0.4% of p-hydroxyacetophenone, 0.5% of 1, 2-hexanediol, 0.05% of ethylhexyl glycerol, 0.2% of water-soluble silicon wax, 0.02% of glutathione and deionized water for complement.
The extraction method of the cordyceps sinensis comprises the following steps:
step 1: selecting a cordyceps sinensis raw material, crushing the cordyceps sinensis raw material, adding a 5% 30% BG aqueous solution, heating to 70 ℃, carrying out water bath for 4 hours, and filtering by using a 1-micron filter paper plate to obtain a cordyceps sinensis stock solution;
step 2: taking the stock solution in the step 1, adding 5 percent BG, and diluting with water to be colorless and transparent
And step 3: and (3) filtering the liquid obtained in the step (2) by using a 0.22-micron filter paper plate to obtain the cordyceps sinensis extract.
The invention uses the high-temperature water bath technology to strengthen the extraction and separation process, can effectively improve the extraction and separation rate, shorten the extraction time, save the cost, and even can improve the quality and the yield of products, and when the cosmetics contain a small amount of cordyceps sinensis extract, the cosmetics have obvious whitening and anti-aging effects.
Example two of the inhibition of tyrosinase activity by Cordyceps sinensis mask fluid:
the purpose is as follows: and (5) measuring the half inhibition concentration of the cordyceps sinensis mask on the tyrosinase activity.
Materials: cordyceps sinensis mask
Sample preparation:
buffer solution: k2HPO4（8.71g/500mL）+KH2PO4（6.805g/500mL）→pH=6.80 （±0.02）
Substrate: about 0.3mg/mL of L-tyrosine
A spectrophotometer: uv, 480nm
The method comprises the following steps:
(1) enzyme concentration selection:
taking a small amount of enzyme, dissolving the enzyme by using a buffer solution, wherein the color is brown; adding 500. mu.L of (L-tyrosine (0.3 mg/mL)), 500. mu.L of buffer solution, 450. mu.L of pure water and 50. mu.L of enzyme into a 1.5mL centrifuge tube; before adding enzyme, water bath is carried out for 20min at 37 ℃; after the enzyme was added, the absorbance was measured at a wavelength of 480nm in a spectrophotometer at 37 ℃ in a water bath (enzyme addition-test) for 10 min. The enzyme concentration was adjusted to give an Absorbance (ABS) of between 0.45 and 0.55. The enzyme solution at this concentration was used as an experimental enzyme solution.
(2) Experimental addition
(3) Measuring absorbance at 480nm, making table, and plotting to obtain IC50。
Calculating the formula: inhibition (%) = [1- (S-SB)/(C-CB) ] + 100
Note that: before adding enzyme, water bath is carried out at 37 ℃ for 20 min; after adding enzyme, water bath at 37 ℃ (adding enzyme-testing time) for 10min for light splitting
The absorbance was measured at a wavelength of 480 nm. Water bath at 37 deg.C for 20min without enzyme.
Results
The results of the cordyceps sinensis mask on inhibiting the tyrosinase activity are shown in the table:
S：Sample SB：Sample Blank C：Control CB：Control Blank
and (4) conclusion: the Cordyceps facial mask has tyrosinase activity inhibiting effect, and IC50 value is about 24 μ L/mL.
Example 3
A whitening mask containing a cordyceps sinensis extract comprises the cordyceps sinensis extract, a mask solution and a biological fiber mask, wherein the mass percent of the cordyceps sinensis extract is 0.1%, and the balance is the mask solution, and the mask solution comprises the following components in percentage by mass: 90% yeast fermentation product filtrate, 0.04% zymosan, 0.14% carbomer 980, 0.12% hydroxyethyl cellulose, 5% glycerol, 0.4% DPG, 0.3% D-panthenol, 0.15% trehalose, 0.6% nicotinamide, 0.1% rhodiola rosea extract, 1.2% tromethamine, 0.09%140 ten thousand molecular weight HA, 2.5% BG, 0.5% p-hydroxyacetophenone, 0.7%1, 2-hexanediol, 0.06% ethylhexyl glycerol, 0.3% water-soluble silicon wax, 0.03% glutathione, and deionized water for complement.
The extraction method of the cordyceps sinensis comprises the following steps:
step 1: selecting a cordyceps sinensis raw material, crushing the cordyceps sinensis raw material, adding a 6% 30% BG aqueous solution, heating to 70 ℃, carrying out water bath for 4 hours, and filtering by using a 1-micron filter paper plate to obtain a cordyceps sinensis stock solution;
step 2: taking the stock solution in the step 1, adding 6 percent BG, and diluting with water to be colorless and transparent
And step 3: and (3) filtering the liquid obtained in the step (2) by using a 0.22-micron filter paper plate to obtain the cordyceps sinensis extract.
The invention uses the high-temperature water bath technology to strengthen the extraction and separation process, can effectively improve the extraction and separation rate, shorten the extraction time, save the cost, and even can improve the quality and the yield of products, and when the cosmetics contain a small amount of cordyceps sinensis extract, the cosmetics have obvious whitening and anti-aging effects.
Example two of the inhibition of tyrosinase activity by Cordyceps sinensis mask fluid:
the purpose is as follows: and (5) measuring the half inhibition concentration of the cordyceps sinensis mask on the tyrosinase activity.
Materials: cordyceps sinensis mask
Sample preparation:
buffer solution: k2HPO4（8.71g/500mL）+KH2PO4（6.805g/500mL）→pH=6.80 （±0.02）
Substrate: about 0.3mg/mL of L-tyrosine
A spectrophotometer: uv, 480nm
The method comprises the following steps:
(1) enzyme concentration selection:
taking a small amount of enzyme, dissolving the enzyme by using a buffer solution, wherein the color is brown; adding 500. mu.L of (L-tyrosine (0.3 mg/mL)), 500. mu.L of buffer solution, 450. mu.L of pure water and 50. mu.L of enzyme into a 1.5mL centrifuge tube; before adding enzyme, water bath is carried out for 20min at 37 ℃; after the enzyme was added, the absorbance was measured at a wavelength of 480nm in a spectrophotometer at 37 ℃ in a water bath (enzyme addition-test) for 10 min. The enzyme concentration was adjusted to give an Absorbance (ABS) of between 0.45 and 0.55. The enzyme solution at this concentration was used as an experimental enzyme solution.
(2) Experimental addition
(3) Measuring absorbance at 480nm, making table, and plotting to obtain IC50。
Calculating the formula: inhibition (%) = [1- (S-SB)/(C-CB) ] + 100
Note that: before adding enzyme, water bath is carried out at 37 ℃ for 20 min; after adding enzyme, water bath at 37 ℃ (adding enzyme-testing time) for 10min for light splitting
The absorbance was measured at a wavelength of 480 nm. Water bath at 37 deg.C for 20min without enzyme.
Results
The results of the cordyceps sinensis mask on inhibiting the tyrosinase activity are shown in the table:
S：Sample SB：Sample Blank C：Control CB：Control Blank
and (4) conclusion: the Cordyceps facial mask has tyrosinase activity inhibiting effect, and IC50 value is about 25.5 μ L/mL.
Thus: the examples show that the cordyceps sinensis facial mask prepared according to the formula and the process of the examples has a remarkable whitening effect. The lowest IC50 value is 24 mu L/mL, namely the product contains 2.4% of cordyceps sinensis extract and has obvious whitening effect
The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements made to the technical solution of the present invention by those skilled in the art without departing from the spirit of the present invention should be included in the protection scope defined by the claims of the present invention.
Claims (3)
1. A whitening mask containing a cordyceps sinensis extract is characterized in that: the facial mask comprises a cordyceps sinensis extract, a facial mask liquid and a biological fiber facial mask, wherein the mass percentage of the cordyceps sinensis extract is 0.05-0.1%, and the balance is the facial mask liquid, and the facial mask liquid is composed of the following raw materials in percentage by weight: 89.96-90% of yeast fermentation product filtrate, 0.02-0.04% of zymosan, 0.1-0.14% of carbomer 980, 0.08-0.12% of hydroxyethyl cellulose, 3-5% of glycerol, 0.2-0.4% of DPG, 0.1-0.3% of D-panthenol, 0.05-0.15% of trehalose, 0.4-0.6% of nicotinamide, 0.05-0.1% of rhodiola rosea extract, 1.0-1.2% of tromethamine, 0.01-0.09% of 140 ten thousand molecular weight HA, 1.5-2.5% of BG, 0.3-0.5% of p-hydroxyacetophenone, 0.3-0.7% of 1, 2-hexanediol, 0.04-0.06% of ethylhexyl glycerol, 0.1-0.3% of silicon wax, 0.01-0.03% of glutathione and the balance of deionized water.
2. The whitening mask containing the cordyceps sinensis extract according to claim 1, wherein the whitening mask comprises: the facial mask liquid contains at least one of humectant, antiseptic, aromatic and hyaluronic acid.
3. The whitening mask containing the cordyceps sinensis extract according to claim 1, wherein the whitening mask comprises: the method for extracting the cordyceps sinensis comprises the following steps:
step 1: selecting a cordyceps sinensis raw material, crushing the cordyceps sinensis raw material, adding 4-6% of 30% BG aqueous solution, heating to 70 ℃, then carrying out water bath for 3-5 hours, and filtering by using a 1-micron filter paper plate to obtain cordyceps sinensis stock solution;
step 2: adding 4-6% BG into the stock solution obtained in the step 1, and diluting the mixture with water until the mixture is colorless and transparent
And step 3: and (3) filtering the liquid obtained in the step (2) by using a 0.22-micron filter paper plate to obtain the cordyceps sinensis extract.
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