CN105233893A - Method for preparing micro-droplets based on micro-fluidic chip modification technology - Google Patents
Method for preparing micro-droplets based on micro-fluidic chip modification technology Download PDFInfo
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Abstract
The invention relates to a method for preparing micro-droplets based on a micro-fluidic chip modification technology. The method adopts a sol-gel method to carry out modification treatment on a micro-fluidic chip. The method has the characteristics of being obvious in modifying effect, simple to operate, and the like; and is suitable for complex-structure or functional micro-droplets such as water-in-oil type micro-droplets, multiple-in-one type micro-droplets, magnetic response type micro-droplets and the like.
Description
Technical field
The invention belongs to micro fluidic chip technical field, relate to a kind of method preparing microlayer model based on micro-fluidic chip modification technology.
Background technology
The making material of micro-fluidic chip has silicon and glass, but because its processing technology is complicated, high in cost of production shortcoming, significantly limit it and apply widely.In recent years, the organic polymer such as polymethyl methacrylate, dimethyl silicone polymer, owing to having high, the good feature such as resistance to ag(e)ing and corrosion resistance of low, lightweight, the easy processing of cost, transparency, has been widely used in the preparation of micro-fluidic chip.Wherein, polymethyl methacrylate is a kind of micro-fluidic chip matrix material comparatively common at present, but due to its own face high hydrophobicity, cause micro-fluidic chip hypofunction or inefficacy, significantly limit the application prospect of polymethyl methacrylate micro flow control chip.Therefore, carry out hydrophilically modifiedly contributing to it and further developing and apply to polymethyl methacrylate micro flow control chip.
The hydrophilically modified method of polymer micro-fluidic chip of current report has: uv irradiation method, cladding process etc.Wherein, uv irradiation method refers to and utilizes UV-irradiation chip material surface a few hours, to improve the hydrophily on surface.Its shortcoming is that the processing time is long, and modified effect is poor, and material may cause the change of surface chemical property again through high temperature or chemical method process, its hydrophily is declined, and affects it and uses repeatability.Cladding process is the chip surface feature of modification as required, selects specific hydrophilic solution to apply chip surface or local, optionally can change the hydrophilic and hydrophobic of chip privileged site.Conventional hydrophilic solution comprises ionic surfactant, such as sldium lauryl sulfate, triethanolamine oleate, sulfated castor oil etc., and nonionic surface active agent, such as polysorbate, glyceryl monooleate, Brij30 etc.Its shortcoming is that apply uneven, obscurity boundary, modified effect is poor to micron-sized fluid channel operating difficulties, even may block fluid channel.
Teos hydrolysis reaction all can occur under acid or alkaline conditions, and acid or alkali play catalytic action in this hydrolysis, determines hydrolysis speed.Comparatively mild as catalyst reaction using acid, produce easy to control, under acid condition, silanol Absorbable organic halogens exists simultaneously, thus improves storage stability.Excessive acid can accelerate silanol (SiOH) or alcoxyl silicon group (SiOR) polycondensation, shortens storage period.On the contrary, subacidity then can separate out SiO
2, reduce SiO in hydrolyzate
2content.In addition, teos hydrolysis is exothermic process, can occur at ambient temperature, there is ethanol to generate simultaneously, can suitably heat up to accelerate hydrolysis reaction, but temperature is no more than 70 DEG C, when temperature is higher than ethyl alcohol boiling point, reaction system easily forms bumping, and ethanol is overflowed in a large number.The interpolation of water is of crucial importance to hydrolysis, does not need ethyl orthosilicate complete hydrolysis because reacting for this reason, therefore needs the addition controlling water.The addition of water too much can cause hydrolyzate hydroxy radical content too much, and easy gel, then hydrolyzate activity is low for the addition deficiency of water.Meanwhile, ethyl orthosilicate is water insoluble, and therefore hydrolysis needs to carry out in organic solvent, usually adopts alcohols, ester class etc., such as ethanol, isopropyl alcohol, n-butanol or their mixture.
For the above-mentioned problems in the prior art, this area is in the urgent need to developing the loaded down with trivial details Process of Surface Modification of the polymethyl methacrylate micro flow control chip of having abandoned prior art, there is the features such as modified effect is obvious, simple to operate, be applicable to the novel method preparing microlayer model based on micro-fluidic chip modification technology preparing water-in-oil type microlayer model and more complicated complex emulsions system.
Summary of the invention
The invention provides a kind of method preparing microlayer model based on micro-fluidic chip modification technology of novelty, thus solve problems of the prior art.
On the one hand, the invention provides a kind of method preparing microlayer model based on micro-fluidic chip modification technology, the method comprises the following steps:
The design of each flaggy of (i) polymethyl methacrylate micro flow control chip: design each layer structure of polymethyl methacrylate micro flow control chip;
(ii) making of each flaggy of polymethyl methacrylate micro flow control chip: independent processing is carried out to each flaggy designed in step (i), and cleans, dry for standby;
(iii) each flaggy of micro-fluidic chip obtained in step (ii) is put in the butanol solution of dilution soak, dry for standby;
(iv) ethyl orthosilicate, deionized water, watery hydrochloric acid and n-butanol dropwise mixed and constantly stir;
V each flaggy of micro-fluidic chip obtained in step (iii) is put in the mixed solution obtained in step (iv) by (), after soaking a period of time, taken out, clean and dry;
(vi) three pieces of flaggies are stacked according to the sequence alignment being followed successively by capping layer, chip layer and basalis from top to bottom, and each flaggy is positioned;
(vii) wrap up three pieces of flaggies behind the location obtained in step (vi), and carry out thermocompression bonding;
(viii), after a hot pressing, upset flaggy, carries out hot pressing again;
(ix) repeatedly repeat step (viii), leave standstill cooling, take out hydrophilic modifying polymethyl methacrylate micro flow control chip; And
X () is chosen that oil-phase solution is decentralized photo, aqueous phase solution is continuous phase, is passed in the micro-fluidic chip of gained, obtain the microlayer model of uniform particle sizes by external pump valve system.
In one preferred embodiment, in step (i), designed chip is the focusing streaming polymethyl methacrylate micro flow control chip that three ply board layer is formed, and it comprises capping layer, chip layer and basalis; Wherein, capping layer is processed with 1 decentralized photo entrance and 2 continuous phase entrances; Chip layer is processed with 1 decentralized photo introduction passage, 1 microlayer model generates chamber and 2 continuous phase introduction passages; Basalis is processed with 1 microlayer model outlet.
Another preferred embodiment in, in step (ii), utilize CO
2laser engraving machine carries out independent processing, and utilizes ultrasonic cleaning; Wherein, when processing capping layer and basalis, CO
2the power of laser engraving machine controls at 90-100%, and cutting speed controls at 50-60%; When processing the microchannel of chip layer, CO
2the power of laser engraving machine controls at 75-80%, and cutting speed controls at 90-100%.
Another preferred embodiment in, in step (iii), described butanol solution is according to n-butanol: the volume ratio 4:3 – 1:1 of deionized water is configured, and soak time controls at 5-10 minute.
Another preferred embodiment in, in step (iv), the volume ratio of described ethyl orthosilicate, deionized water, 0.1mol/L watery hydrochloric acid and n-butanol four kinds of solution is about (1-1.5): (3-4): (1-2): (1-4), wherein ethyl orthosilicate, deionized water and n-butanol first carry out mixing and constantly stir, are more dropwise added wherein by watery hydrochloric acid.
Another preferred embodiment in, in step (v), described soak time is no less than 3 hours, with deionized water clean.
Another preferred embodiment in, in step (vi), described three pieces of flaggies adopt micro-ultra-violet curing glue to fix, and the ultra-violet curing time is 10-60 second.
Another preferred embodiment in, in step (vii), wrap up with masking foil, in hot press, carry out thermocompression bonding, the temperature of thermocompression bonding is 90-105 DEG C, and each hot pressing time is 40-60 second.
Another preferred embodiment in, in step (ix), upset hot pressing number of times is no less than 3 times, and standing cool time is 1-2 minute, takes out hydrophilic modifying polymethyl methacrylate micro flow control chip from masking foil.
Another preferred embodiment in, in step (x), described microlayer model be decentralized photo oil-phase solution in focused flow passage, by the continuous phase aqueous phase solution of two sides converge shear and formed; Wherein, change the component of decentralized photo or the flow-rate ratio of two-phase, obtain labyrinth or functional microlayer model further.
Accompanying drawing explanation
Fig. 1 is the structural representation according to polymethyl methacrylate micro flow control chip of the present invention.
Fig. 2 is the process chart that porous polymer particle prepared by micro-fluidic chip experimental rig according to the present invention.
Fig. 3 be according to micro-fluidic chip of the present invention before modified after contact angle photo.
Detailed description of the invention
Present inventor finds after extensive and deep research, for this technical problem of Process of Surface Modification that the polymethyl methacrylate micro flow control chip of prior art is loaded down with trivial details, sol-gel process is adopted to carry out modification to micro-fluidic chip, there is the features such as modified effect is obvious, simple to operate, be applicable to prepare water-in-oil type microlayer model and more complicated complex emulsions system.Based on above-mentioned discovery, the present invention is accomplished.
The invention provides a kind of method preparing microlayer model based on micro-fluidic chip modification technology, the method comprises the following steps:
Step one: the design of each flaggy of polymethyl methacrylate micro flow control chip: design each layer structure of polymethyl methacrylate micro flow control chip;
Step 2: the making of each flaggy of polymethyl methacrylate micro flow control chip: utilize CO
2laser engraving machine carries out independent processing to each flaggy designed by step one, and ultrasonic cleaning, dry for standby;
Step 3: each flaggy of micro-fluidic chip step 2 obtained is put in the butanol solution diluted according to a certain ratio and soaked, dry for standby;
Step 4: by the dropwise mixing also constantly stirring by a certain percentage of ethyl orthosilicate, deionized water, watery hydrochloric acid, n-butanol;
Step 5: put in the mixed solution of step 4 gained by each for the micro-fluidic chip obtained in step 3 flaggy, after soaking a period of time, is taken out, cleans and dry by deionized water;
Step 6: by three pieces of flaggies according to be followed successively by from top to bottom capping layer, chip layer, basalis sequence alignment stack, and with micro-ultra-violet curing glue, each flaggy to be positioned;
Step 7: with three pieces of flaggies behind the location that obtains in masking foil parcel step 6, and be placed in hot press and carry out bonding;
Step 8: after a hot pressing, upset flaggy, carries out hot pressing again;
Step 9: repeatedly repeat step 8, leaves standstill cooling, takes out hydrophilic modifying polymethyl methacrylate micro flow control chip from masking foil;
Step 10: choose that oil-phase solution is decentralized photo, aqueous phase solution is continuous phase, is passed in above-mentioned micro-fluidic chip by external pump valve system, is obtained the micro-droplet of oil of uniform particle sizes.
In the present invention, chip designed in step one is the focusing streaming polymethyl methacrylate micro flow control chip that 3 laminate layers are formed, and comprises capping layer, chip layer and basalis, can realize the continuous production of water-in-oil type microlayer model; Wherein capping layer is processed with 1 decentralized photo entrance and 2 continuous phase entrances; Chip layer is processed with microchannel, and drop size is determined by microchannel size; Basalis is processed with 1 drop and collects outlet.
In the present invention, in step 2 when processing capping layer and basalis, CO
2laser engraving acc power controls at 90-100%, and cutting speed controls at 50-60%; When processing chip layer microchannel, CO
2laser engraving acc power controls at 75-80%, and cutting speed controls at 90-100%, and to prevent power excessive, speed is slow, and laser burn plate, expands the size of microchannel, increases the surface roughness at edge, microchannel simultaneously.
In the present invention, the butanol solution in step 3 is according to n-butanol: deionized water volume ratio 4:3 – 1:1, preferred 1:0.75 is configured, and soak time controls at 5-10 minute.
In the present invention, the volume ratio of the ethyl orthosilicate described in step 4, deionized water, watery hydrochloric acid (0.1mol/L), n-butanol four kinds of solution is about (1-1.5): (3-4): (1-2): (1-4), preferred 1:4:2:4, wherein ethyl orthosilicate, deionized water and n-butanol first carry out mixing and constantly stir, are more dropwise added wherein by watery hydrochloric acid; Ethyl orthosilicate is under sour environment, and room temperature hydrolysis can occur, and uses 40-60 DEG C of water-bath, can facilitation of hydrolysis reaction generation.
In the present invention, the soak time described in step 5 is no less than 3 hours.
In the present invention, piece flaggy of three described in step 6 adopts micro-ultra-violet curing glue to fix, and the ultra-violet curing time is 10-60 second.
In the present invention, the thermocompression bonding temperature in step 7 is about 90-105 DEG C, and each hot pressing time is about 40-60 second.
In the present invention, the upset hot pressing number of times described in step 9 is no less than 3 times, and standing cool time is about 1-2 minute.
In the present invention, the microlayer model described in step 10 be decentralized photo oil-phase solution in focused flow passage, by the continuous phase aqueous phase solution of two sides converge shear and formed; Wherein, change the component of decentralized photo or the flow-rate ratio of two-phase, water-in-oil type can be obtained further, magnetic response type, wraps labyrinth or functional microlayer model such as many types of.
Below referring to accompanying drawing.
Fig. 1 is the structural representation according to polymethyl methacrylate micro flow control chip of the present invention.As shown in Figure 1, described polymethyl methacrylate micro flow control chip comprises capping layer A, chip layer B and basalis C; Wherein, capping layer A is processed with 1 decentralized photo entrance a and 2 continuous phase entrance f; Chip layer B is processed with 1 decentralized photo introduction passage b, 1 microlayer model generates chamber d and 2 continuous phase introduction passage e; Basalis is processed with 1 microlayer model outlet c.
Fig. 2 is the process chart that porous polymer particle prepared by micro-fluidic chip experimental rig according to the present invention.As shown in Figure 2, use syringe pump 1 (Lange integral type Laboratary type syringe pump, LSP01-2A), by hydrogen peroxide solution and 1,6-hexanediyl ester solution 1:2 mixing by volume also high-speed stirred, obtain decentralized photo solution; Use syringe pump 2 (Lange integral type Laboratary type syringe pump, LSP02-1B), will the glycerin solution of surfactant be added with as continuous phase solution; Getting above-mentioned decentralized photo solution 10mL and continuous phase solution 15mL is passed in micro-fluidic chip with velocity ratio 1:4, obtains water-in-oil type microlayer model; Drop is collected outlet by the drop of basalis and is flowed out, and collects in the culture dish being added with the collection phase the same with continuous phase component; Last under ultraviolet light uviol lamp, irradiation is after 30 minutes, and polymerization, solidification, cleaning-drying, obtains the small porous particle of uniform particle sizes.
Fig. 3 be according to micro-fluidic chip of the present invention before modified after contact angle photo.As shown in Figure 3, contact angle is 76.178 ° before modified, and modified contact angle obviously reduces, and is 40.143 °.
Major advantage of the present invention is:
The present invention proposes a kind of method preparing microlayer model based on micro-fluidic chip modification technology, adopt sol-gel process to the process of micro-fluidic chip modifying surface, there is the features such as modified effect is obvious, simple to operate.According to the multiple-layer stacked micro-fluidic chip that this method of modifying designs, can to every Rotating fields independent design processing of chip, functional structure in chip is formed again through being layering, finally realize the preparation of the complex emulsions of labyrinth, the preparation of functional particle, the application such as the compartment analysis of protein and amino acid, organic-inorganic Small molecular and metal ion.
embodiment
The present invention is set forth further below in conjunction with specific embodiment.But, should be understood that these embodiments only do not form limitation of the scope of the invention for illustration of the present invention.The test method of unreceipted actual conditions in the following example, usually conveniently condition, or according to the condition that manufacturer advises.Except as otherwise noted, all percentage and number are by weight.
embodiment 1: utilize the polymethyl methacrylate micro flow control chip of modification of the present invention to prepare water-in-oil type micro-
drop
Concrete steps are as follows:
Step one: the design of polymethyl methacrylate micro flow control chip: design each flaggy of polymethyl methacrylate micro flow control chip, designed chip is made up of 3 pieces of flaggies, they are capping layer, chip layer and basalis respectively, wherein capping layer is processed with 1 decentralized photo entrance and 2 continuous phase entrances, chip layer is processed with microchannel, and basalis is processed with 1 drop and collects outlet;
Step 2: the making of polymethyl methacrylate micro flow control chip: utilize CO
2each flaggy described in laser engraving machining steps one; During processing capping layer, CO
2laser engraving acc power is 95%, and cutting speed is 50%; During processing chip layer, CO
2laser engraving acc power is 77%, and cutting speed is 95%; Each flaggy ultrasonic cleaning 10 minutes, dry for standby;
Step 3: each flaggy of micro-fluidic chip step 2 obtained is put in the n-butanol of 1:0.75 dilution and soaked after 5 minutes, takes out dry for standby;
Step 4: by ethyl orthosilicate, deionized water, watery hydrochloric acid (0.1mol/L), n-butanol by volume 1:4:1.6:4 dropwise mix under 50 DEG C of water bath condition; Wherein ethyl orthosilicate, deionized water and n-butanol first carry out mixing and constantly stir, are more dropwise dripped by watery hydrochloric acid;
Step 5: put in the mixed solution of step 4 gained by each for the micro-fluidic chip obtained in step 3 flaggy, soaks after 3 hours, is taken out, clean and dry by deionized water;
Step 6: by three pieces of flaggies according to be followed successively by from top to bottom capping layer, chip layer, basalis sequence alignment stack, and with trace ultra-violet curing glue its top and bottom are fixed respectively, 45 seconds ultra-violet curing time;
Step 7: with three pieces of flaggies behind the location that obtains in masking foil parcel step 6, and be placed in hot press and carry out bonding; Hot pressing temperature is 87 DEG C, and each hot pressing time is 40 seconds;
Step 8: after a hot pressing, upset flaggy, carries out hot pressing again;
Step 9: after repeating step 84 times, take out from hot press, leaves standstill cooling 2 minutes, removes masking foil, obtains hydrophilic modifying polymethyl methacrylate micro flow control chip.
The hydrophilic modifying polymethyl methacrylate micro flow control chip of gained describes: chip water white transparency, is made up of and focuses on streaming polymethyl methacrylate micro flow control chip, have good optical characteristics and mechanical strength 3 laminate layers.Chip overall dimensions is 80mm × 40mm × 6mm, and every laminate layer thickness is 2mm; Wherein the decentralized photo of capping layer and continuous phase entrance hole diameter are 2mm, and it is 2mm that the drop of basalis collects outlet aperture, and decentralized photo introduction passage and continuous phase introduction passage width are 2mm, and focused flow drop formation channel width is 0.5mm.
The application of the hydrophilic modifying polymethyl methacrylate micro flow control chip of gained: decentralized photo deionized water (deionized water 5mL, surfactant F-127 (Sigma-AldrichCo.LLC.) 0.05g) and continuous phase GDMA solution (GDMA 10g, ricinoleic acid polyglycerol ester 0.5g) are injected in micro-fluidic chip with flow-rate ratio 1:4 by the entrance of correspondence, obtain a series of water-in-oil type microlayer model.Drop is collected outlet by the drop of basalis and is flowed out, and collects in the culture dish being added with the collection phase the same with continuous phase component.
embodiment 2: utilize the polymethyl methacrylate micro flow control chip of modification of the present invention to prepare a bag many types of micro-
drop
Concrete steps are as follows:
Hydrophilic modifying polymethyl methacrylate micro flow control chip is made by described in embodiment 1.
By deionized water and vegetable oil by volume 5:1 carry out high-speed stirred, mixing speed is 18000rpm, and mixing time is 5 minutes, obtains decentralized photo solution; Get the above-mentioned decentralized photo solution of 5mL and 10mL continuous phase solution (GDMA 10g, ricinoleic acid polyglycerol ester 0.5g) is injected in micro-fluidic chip with flow-rate ratio 1:5 by the entrance of correspondence, obtain the many types of microlayer model of an a series of bag.Drop is collected outlet by the drop of basalis and is flowed out, and collects in the culture dish being added with the collection phase the same with continuous phase component.
embodiment 3: utilize the polymethyl methacrylate micro flow control chip of modification of the present invention to prepare porous polymer
particle
Concrete steps are as follows:
Hydrophilic modifying polymethyl methacrylate micro flow control chip is made by described in embodiment 1.
As shown in Figure 2, by hydrogen peroxide solution (hydrogen peroxide 10mL, surfactant F-127 (Sigma-AldrichCo.LLC.) 0.1g) and 1,6-hexanediyl ester solution (1,6-hexanediyl ester 10g, ricinoleic acid polyglycerol ester 0.5g, light trigger HMPP (Shanghai Aladdin biochemical technology limited company) 0.1g) the also high-speed stirred of 1:2 mixing by volume, mixing speed is 25000rpm, mixing time is 3 minutes, obtains decentralized photo solution; To the glycerin solution (glycerine 2g, deionized water 40mL, surfactant F-1270.4g) of surfactant F-127 be added with as continuous phase solution.Getting above-mentioned decentralized photo solution 10mL and continuous phase solution 15mL is passed in micro-fluidic chip with velocity ratio 1:4, obtains complex emulsions drop.Drop is collected outlet by the drop of basalis and is flowed out, and collects in the culture dish being added with the collection phase the same with continuous phase component.Last under ultraviolet light uviol lamp (400W, 365nm), irradiation is after 30 minutes, and cleaning-drying, obtains the porous polymer particles of uniform particle sizes, and average grain diameter is 463 μm.
Above-mentioned listed embodiment is only preferred embodiment of the present invention, is not used for limiting practical range of the present invention.Namely all equivalences done according to the content of the present patent application the scope of the claims change and modify, and all should be technology category of the present invention.
The all documents mentioned in the present invention are quoted as a reference all in this application, are just quoted separately as a reference as each section of document.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after having read above-mentioned instruction content of the present invention.
Claims (10)
1. prepare a method for microlayer model based on micro-fluidic chip modification technology, the method comprises the following steps:
The design of each flaggy of (i) polymethyl methacrylate micro flow control chip: design each layer structure of polymethyl methacrylate micro flow control chip;
(ii) making of each flaggy of polymethyl methacrylate micro flow control chip: independent processing is carried out to each flaggy designed in step (i), and cleans, dry for standby;
(iii) each flaggy of micro-fluidic chip obtained in step (ii) is put in the butanol solution of dilution soak, dry for standby;
(iv) ethyl orthosilicate, deionized water, watery hydrochloric acid and n-butanol dropwise mixed and constantly stir;
V each flaggy of micro-fluidic chip obtained in step (iii) is put in the mixed solution obtained in step (iv) by (), after soaking a period of time, taken out, clean and dry;
(vi) three pieces of flaggies are stacked according to the sequence alignment being followed successively by capping layer, chip layer and basalis from top to bottom, and each flaggy is positioned;
(vii) wrap up three pieces of flaggies behind the location obtained in step (vi), and carry out thermocompression bonding;
(viii), after a hot pressing, upset flaggy, carries out hot pressing again;
(ix) repeatedly repeat step (viii), leave standstill cooling, take out hydrophilic modifying polymethyl methacrylate micro flow control chip; And
X () is chosen that oil-phase solution is decentralized photo, aqueous phase solution is continuous phase, is passed in the micro-fluidic chip of gained, obtain the microlayer model of uniform particle sizes by external pump valve system.
2. the method for claim 1, it is characterized in that, in step (i), designed chip is the focusing streaming polymethyl methacrylate micro flow control chip that three ply board layer is formed, and it comprises capping layer (A), chip layer (B) and basalis (C); Wherein, capping layer (A) is processed with 1 decentralized photo entrance (a) and 2 continuous phases entrance (f); Chip layer (B) is processed with 1 decentralized photo introduction passage (b), 1 microlayer model generates chamber (d) and 2 continuous phases introduction passage (e); Basalis is processed with 1 microlayer model outlet (c).
3. method as claimed in claim 1 or 2, is characterized in that, in step (ii), utilize CO
2laser engraving machine carries out independent processing, and utilizes ultrasonic cleaning; Wherein, when processing capping layer (A) and basalis (C), CO
2the power of laser engraving machine controls at 90-100%, and cutting speed controls at 50-60%; When processing the microchannel of chip layer (B), CO
2the power of laser engraving machine controls at 75-80%, and cutting speed controls at 90-100%.
4. method as claimed in claim 1 or 2, it is characterized in that, in step (iii), described butanol solution is according to n-butanol: the volume ratio 4:3 – 1:1 of deionized water is configured, and soak time controls at 5-10 minute.
5. method as claimed in claim 1 or 2, it is characterized in that, in step (iv), the volume ratio of described ethyl orthosilicate, deionized water, 0.1mol/L watery hydrochloric acid and n-butanol four kinds of solution is (1-1.5): (3-4): (1-2): (1-4), wherein ethyl orthosilicate, deionized water and n-butanol first carry out mixing and constantly stir, are more dropwise added wherein by watery hydrochloric acid.
6. method as claimed in claim 1 or 2, it is characterized in that, in step (v), described soak time is no less than 3 hours, cleans by deionized water.
7. method as claimed in claim 1 or 2, it is characterized in that, in step (vi), described three pieces of flaggies adopt micro-ultra-violet curing glue to fix, and the ultra-violet curing time is 10-60 second.
8. method as claimed in claim 1 or 2, it is characterized in that, in step (vii), wrap up with masking foil, in hot press, carry out thermocompression bonding, the temperature of thermocompression bonding is 90-105 DEG C, and each hot pressing time is 40-60 second.
9. method as claimed in claim 1 or 2, is characterized in that, in step (ix), upset hot pressing number of times is no less than 3 times, and standing cool time is 1-2 minute, takes out hydrophilic modifying polymethyl methacrylate micro flow control chip from masking foil.
10. method as claimed in claim 1 or 2, is characterized in that, in step (x), described microlayer model be decentralized photo oil-phase solution in focused flow passage, to be sheared by the continuous phase aqueous phase solution of two sides converge and to be formed; Wherein, change the component of decentralized photo or the flow-rate ratio of two-phase, obtain labyrinth or functional microlayer model further.
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| CN113058516A (en) * | 2021-03-18 | 2021-07-02 | 江苏师范大学 | Preparation method of microfluidic complex liquid drop induced by liquid-liquid phase separation |
| CN113145190A (en) * | 2021-04-27 | 2021-07-23 | 华东理工大学 | Microfluidic device for large-scale controllable preparation of multi-structure composite micro-droplets |
| CN115521882A (en) * | 2021-06-24 | 2022-12-27 | 清华大学 | Micro-upgrading single-cell droplet generation and culture method and device |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1616543A (en) * | 2003-11-11 | 2005-05-18 | 中国科学院大连化学物理研究所 | Hydrophilic poly methyl methacrylate chip material, chip and its preparing method |
| CN1632547A (en) * | 2004-12-23 | 2005-06-29 | 复旦大学 | Method for preparing noumenal modification polymethyl methacrylate micro flow control chip |
| CN101042396A (en) * | 2007-04-19 | 2007-09-26 | 复旦大学 | Method for modifying surface silica gel on organic glass micro-fluidic chip channel |
| CN101570776A (en) * | 2008-04-29 | 2009-11-04 | 中国科学院大连化学物理研究所 | High-throughput nano-litre micro-droplet forming and fixing method based on microfluidic chip and special chip and application thereof |
| CN102153774A (en) * | 2011-01-27 | 2011-08-17 | 济南大学 | Surface-finish method of PMMA (Polymethylmethacrylate) micro-fluidic chip, micro-fluidic coated chip and application thereof |
| CN102190287A (en) * | 2011-03-24 | 2011-09-21 | 大连理工大学 | Method for raising hot pressing bonding rate of PMMA micro fluidic chip formed by injection moulding |
| CN103232613A (en) * | 2013-04-02 | 2013-08-07 | 陕西师范大学 | Modification method for surface of poly(methyl methacrylate) microfluidic chip by using amphiphilic oligopeptide |
| CN103386333A (en) * | 2013-08-07 | 2013-11-13 | 苏州扬清芯片科技有限公司 | Micro-fluidic liquid drop production chip |
| CN104511320A (en) * | 2013-09-27 | 2015-04-15 | 王来 | A liquid-drop-generation capillary microfluidic chip and a preparing method thereof |
| CN104607257A (en) * | 2015-01-15 | 2015-05-13 | 安徽理工大学 | Micro-fluidic chip surface hydrophilic modification treatment method and hydrophilic surface gradient manufacture method |
| CN104741089A (en) * | 2015-02-15 | 2015-07-01 | 华东理工大学 | Method for preparing crosslinked-chitosan-system azo dye wastewater adsorbent |
-
2015
- 2015-11-02 CN CN201510733153.1A patent/CN105233893A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1616543A (en) * | 2003-11-11 | 2005-05-18 | 中国科学院大连化学物理研究所 | Hydrophilic poly methyl methacrylate chip material, chip and its preparing method |
| CN1632547A (en) * | 2004-12-23 | 2005-06-29 | 复旦大学 | Method for preparing noumenal modification polymethyl methacrylate micro flow control chip |
| CN101042396A (en) * | 2007-04-19 | 2007-09-26 | 复旦大学 | Method for modifying surface silica gel on organic glass micro-fluidic chip channel |
| CN101570776A (en) * | 2008-04-29 | 2009-11-04 | 中国科学院大连化学物理研究所 | High-throughput nano-litre micro-droplet forming and fixing method based on microfluidic chip and special chip and application thereof |
| CN102153774A (en) * | 2011-01-27 | 2011-08-17 | 济南大学 | Surface-finish method of PMMA (Polymethylmethacrylate) micro-fluidic chip, micro-fluidic coated chip and application thereof |
| CN102190287A (en) * | 2011-03-24 | 2011-09-21 | 大连理工大学 | Method for raising hot pressing bonding rate of PMMA micro fluidic chip formed by injection moulding |
| CN103232613A (en) * | 2013-04-02 | 2013-08-07 | 陕西师范大学 | Modification method for surface of poly(methyl methacrylate) microfluidic chip by using amphiphilic oligopeptide |
| CN103386333A (en) * | 2013-08-07 | 2013-11-13 | 苏州扬清芯片科技有限公司 | Micro-fluidic liquid drop production chip |
| CN104511320A (en) * | 2013-09-27 | 2015-04-15 | 王来 | A liquid-drop-generation capillary microfluidic chip and a preparing method thereof |
| CN104607257A (en) * | 2015-01-15 | 2015-05-13 | 安徽理工大学 | Micro-fluidic chip surface hydrophilic modification treatment method and hydrophilic surface gradient manufacture method |
| CN104741089A (en) * | 2015-02-15 | 2015-07-01 | 华东理工大学 | Method for preparing crosslinked-chitosan-system azo dye wastewater adsorbent |
Non-Patent Citations (2)
| Title |
|---|
| 杨波 等: "一种微流控检测芯片的设计与工艺研究", 《仪器仪表学报》 * |
| 霍玉秋 等: "3种共溶剂对正硅酸乙酯水解的影响", 《东北大学学报（自然科学版）》 * |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105498875A (en) * | 2016-01-27 | 2016-04-20 | 杭州霆科生物科技有限公司 | Centrifugal micro-fluidic chip for preparing liquid drops |
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| CN106345545A (en) * | 2016-09-26 | 2017-01-25 | 苏州汶颢芯片科技有限公司 | Multinuclear emulsion drip preparation chip and modification method |
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| CN108031975A (en) * | 2017-10-24 | 2018-05-15 | 广东工业大学 | A kind of induced with laser implantation preparation method of continuous multilayer drop parcel |
| CN108940392A (en) * | 2018-07-13 | 2018-12-07 | 苏州大学张家港工业技术研究院 | Drop driving method and device for composite digital micro-fluidic chip |
| CN108940392B (en) * | 2018-07-13 | 2021-08-17 | 苏州大学张家港工业技术研究院 | Liquid drop driving method and device for composite digital microfluidic chip |
| CN111252751A (en) * | 2018-12-03 | 2020-06-09 | 成都市银隆新能源有限公司 | Microfluidic droplet forming structural component and method for preparing solid spherical lithium iron phosphate |
| CN110044774A (en) * | 2019-04-24 | 2019-07-23 | 中国石油大学(北京) | Emulsify the micro fluidic device and method for improving recovery ratio research in situ for surfactant |
| CN110152748A (en) * | 2019-06-12 | 2019-08-23 | 河北工业大学 | A method of it is cut for microlayer model in micro-fluidic chip |
| CN112588222A (en) * | 2020-11-25 | 2021-04-02 | 浙江大学 | Preparation device and method of porous polymer with porosity and arrangement regulated and controlled by surface acoustic waves |
| CN112588222B (en) * | 2020-11-25 | 2022-02-18 | 浙江大学 | Preparation device and method of porous polymer with porosity and arrangement regulated and controlled by surface acoustic waves |
| CN113058516A (en) * | 2021-03-18 | 2021-07-02 | 江苏师范大学 | Preparation method of microfluidic complex liquid drop induced by liquid-liquid phase separation |
| CN113145190A (en) * | 2021-04-27 | 2021-07-23 | 华东理工大学 | Microfluidic device for large-scale controllable preparation of multi-structure composite micro-droplets |
| CN115521882A (en) * | 2021-06-24 | 2022-12-27 | 清华大学 | Micro-upgrading single-cell droplet generation and culture method and device |
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