CN105233118A - Stype type medicine formula treating vaginitis of department of gynecology and preparation method thereof - Google Patents
Stype type medicine formula treating vaginitis of department of gynecology and preparation method thereof Download PDFInfo
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- CN105233118A CN105233118A CN201510798356.9A CN201510798356A CN105233118A CN 105233118 A CN105233118 A CN 105233118A CN 201510798356 A CN201510798356 A CN 201510798356A CN 105233118 A CN105233118 A CN 105233118A
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Abstract
The invention discloses a stype type medicine formula treating vaginitis of the department of gynecology and a preparation method thereof. The stype type medicine formula treating vaginitis of the department of gynecology is prepared from, by weight, 21-29 parts of indigo naturalis, 18-24 parts of acorus tatarinowii, 15-19 parts of scaphium scaphigerum, 10-13 parts of parched pangolin scales, 8-12 parts of Chinese trumpet creeper, 8-12 parts of pubescent holly roots, 5-9 parts of angelica roots, 5-9 parts of phaseolus calcaratus, 3-6 parts of bombyx batryticatus, 3-6 parts of clematis chinensis, 5-8 parts of asarum, 4-7 parts of musk, 28-38 parts of xylitol, 58-88 parts of gum bases and 5-19 parts of mint. The stype type medicine formula treating vaginitis of the department of gynecology has the following advantages of being high in fungicidal efficiency, long in attachment time, long in medicine playing time and capable of promoting the qi and blood treatment period to be short.
Description
Technical field
The present invention relates to a kind of gynecological inflammation healing potion and preparation method thereof, is that one treats gynecology colpitis hydrant reagent combination and preparation method thereof specifically.
Background technology
The cause of disease
1. bacterial vaginosis
Preponderate with the lactobacillus producing hydrogen peroxide in normal vagina.During bacterial vaginosis, the endogenous mixed infection caused by increase such as lactobacillus minimizing, Gardnerella and anaerobe per vaginam.
2. monilial vaginitis
(1) 80% ~ 90% pathogen is candida albicans bacterium, and sour environment is easy to growth, is two-phase bacterium (Yeast Phase, Hyphal form).
(2) patient's vagina pH is 4.0 ~ 4.7, normal <4.5.
(3) conditioned pathogen (Yeast Phase → Hyphal form).
(4) common inducement: gestation, diabetes, extensive application immunosuppressant and broad ectrum antibiotic.
(5) other inducements: gastrointestinal tract candidiasis, close-fitting chemical fibre underwear, overweight people.
3. trichomonal vaginitis
(1) trichomonal vaginitis is suitable for growing in the wet environment of temperature 25 DEG C ~ 40 DEG C, pH5.2 ~ 6.6.
(2) before and after menstruation, vagina pH changes, and close to neutral after menstruation, infusorian easily breeds.
(3) the vagina pH of patient is generally 5.0 ~ 6.5, most >6.0.
(4) vagina, urethra or paraurethral gland, bladder, renal pelvis, bridegroom's or husband's side foreskin fold, urethra, prostate is parasitized.
(5) normal and other vaginitiss depositing.
4. senile vaginitis
Postmenopausal women is because of ovarian failure, and estrogen level reduces, vaginal wall atrophy, and mucosa is thinning, and intravaginal pH increases, and local resistance reduces, and other pathogenic bacterium excessive multiplications or easily invasion cause inflammation, based on aerobe.
Clinical manifestation
1. bacterial vaginosis
10% ~ 40% patient is without clinical symptoms, and have symptom's main manifestations to be that vaginal secretions increases, have a fish like smell, especially sexual intercourse postemphasis, can with slight pruritus vulvae or burning sensation.Check and see that vaginal mucosa shows without the inflammation of hyperemia, secretions feature is canescence, and uniformity is thin, and length attaches to vaginal wall, is easily wiped away from vaginal wall by secretions.
2. monilial vaginitis
(1) pruritus vulvae, causalgia, sexual anhedonia.
(2) frequent micturition, dysurea.Dysurea feature is that when urinating, urine stimulates the pudendum of edema and vestibule to cause pain.
(3) feature secretions: white stiff is curdled milk or bean dregs sample.
(4) vulvitis-ground pattern erythema, edema, scratch.
(5) vaginitis-edema, erythema, white membranoid substance.
3. trichomonal vaginitis
(1) vaginal secretions increases feature: thin purulence, yellow green, cystose, frowziness.
(2) pruritus vulvae position: vaginal orifice and pudendum.
(3) if merge urinary tract infection: frequent micturition, urgent micturition, dysurea, visible hematuria sometimes.
(4) infertile: trichomonal vaginitis can engulf sperm, hinder lactic acid to generate, affect it and survive in intravaginal.
(5) check and see: vagina glues mucosa hyperemia, is dispersed in hemorrhage speckle, arched roof volume leucorrhea after " Fructus Fragariae Ananssae sample " cervix uteri, in lark, yellow-white wash or yellow green purulent secretion, normal show bubble.The change without exception of carrier's vaginal mucosa.
4. senile vaginitis
Vaginal secretions increases, pruritus vulvae etc., often with sexual anhedonia.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of convenient drug administration, has outstanding bacteria growing inhibiting, and antiinflammatory promotes gynecology colpitis healing potion of cellular metabolism and preparation method thereof.
For solving the problems of the technologies described above, technical scheme of the present invention is: one treats gynecology colpitis hydrant reagent combination, be made up of the raw material of following weight portion: be made up of the raw material of following weight portion: Radix Stemonae 10-13 part, Flos Chrysanthemi Indici 11-14 part, Herba Violae 11-14 part, Folium Eucalypti Robustae 11-15 part, Rhizoma Corydalis 10-14 part, Indigo Naturalis 7-10 part, Camphora 7-12 part, Borneolum Syntheticum 6-9 part, Myrrha 23-25 part, Olibanum 21-25 part, catechu 20-25 part, Flos Lonicerae 20-25 part, Radix Glycyrrhizae 20-24 part, Radix Angelicae Sinensis 10-13 part, Flos Carthami 10-13 part, aloe gel 40:1-56 parts, gel 47-68 part.
As preferred technical scheme, described one treats gynecology colpitis hydrant reagent combination, is made up of following raw material: Radix Stemonae 11.5 parts, Flos Chrysanthemi Indici 12.5 parts, Herba Violae 12.5 parts, Folium Eucalypti Robustae 13 parts, Rhizoma Corydalis 12 parts, Indigo Naturalis 8.5 parts, Camphora 9.5 parts, Borneolum Syntheticum 7.5 parts, Myrrha 23 parts, Olibanum 23 parts, 22.5 parts, catechu, Flos Lonicerae 22.5 parts, 22 parts, Radix Glycyrrhizae, Radix Angelicae Sinensis 11.5 parts, 11.5 parts, Flos Carthami, Aloe gel 48 parts, gel 52.5 parts.
Present invention also offers above-mentioned a kind of manufacture method for the treatment of gynecology colpitis hydrant reagent combination, comprise the following steps:
Step one, powder process
Pour Radix Stemonae, Flos Chrysanthemi Indici, Herba Violae, Folium Eucalypti Robustae, Rhizoma Corydalis, Indigo Naturalis, Camphora, Borneolum Syntheticum, Myrrha, Olibanum, catechu, Flos Lonicerae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Flos Carthami into frying pan, add 8 times of decoctings again to endure, first seethe with excitement and decoct 2-3 hour at slow fire, stop adding when medicine soup quality is 1.3 times of medical material gross mass, medicinal residues are leached, obtains medicine soup;
Step 2
The spray-dried machine of medicine soup that step one is obtained, removing moisture obtains medicated powder;
Step 3
Gelatin is melted, and keeps temperature, make it maintain melting state for subsequent use;
Step 4
Aloe gel is poured in blender, and Aloe gel is heated to 52.3 degrees Celsius, and maintain this temperature, then the melted gelatin of medicated powder step 2 obtained and step 3 acquisition adds in Aloe gel, fully stirs and obtains mixed solution;
Step 5, molding
Before mixed solution step 4 obtained is to be cooled, pours mould into and make stype;
Step 4, packaging
Cooled stype is poured out mould, and load in special syringe, pack, cold preservation is for subsequent use.
Owing to have employed technique scheme, one treats gynecology colpitis hydrant reagent combination, is made up of following raw material: Radix Stemonae 10-13 part, Flos Chrysanthemi Indici 11-14 part, Herba Violae 11-14 part, Folium Eucalypti Robustae 11-15 part, Rhizoma Corydalis 10-14 part, Indigo Naturalis 7-10 part, Camphora 7-12 part, Borneolum Syntheticum 6-9 part, Myrrha 23-25 part, Olibanum 21-25 part, catechu 20-25 part, Flos Lonicerae 20-25 part, Radix Glycyrrhizae 20-24 part, Radix Angelicae Sinensis 10-13 part, Flos Carthami 10-13 part, aloe gel 40:1-56 parts, gel 47-68 part; The present invention has following beneficial effect:
1, kill antibacterial with Radix Stemonae, Flos Chrysanthemi Indici, Herba Violae, Folium Eucalypti Robustae, Rhizoma Corydalis, Indigo Naturalis, Camphora, discharge toxin, reach the object for the treatment of inflammation.
2, collocation Myrrha, Olibanum, catechu, Flos Lonicerae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Flos Carthami promote vim and vigour circulation, promote cellular metabolism, new cells produce, discharge necrosis, cell of dying of illness, be combined effectively improve tissue growth environment with expellant medicament, improve immunity of organisms.
3, the highly dissoluble of Aloe gel and bacteriostasis are arranged in pairs or groups the adhesion of gelatin again, make medicine better depend on disease place, make medicine play the time longer, coordinate qi and blood circulation promotion medicine, make drug utilization maximum effect, promote therapeutic effect and Shorten the Treatment Process.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described further.
embodiment 1
One treats gynecology colpitis hydrant reagent combination, is made up of following raw material: Indigo Naturalis 21 parts, Rhizoma Acori Graminei 18 parts, Semen Sterculiae Lychnophorae 15 parts, stir-baked SQUAMA MANITIS 10 parts, Flos Campsis 8 parts, Radix Ilicis Pubescentis 8 parts, the Radix Angelicae Dahuricae 5 parts, Semen Phaseoli 5 parts, Bombyx Batryticatus 3 parts, Radix Clematidis 3 parts, Herba Asari 5 parts, 4 parts, Moschus, xylitol 28 parts, gum base: 58 parts, Herba Menthae 5 parts.
Above-mentioned a kind of manufacture method for the treatment of gynecology colpitis hydrant reagent combination, comprises the following steps:
Step one, powder process
Pour Radix Stemonae, Flos Chrysanthemi Indici, Herba Violae, Folium Eucalypti Robustae, Rhizoma Corydalis, Indigo Naturalis, Camphora, Borneolum Syntheticum, Myrrha, Olibanum, catechu, Flos Lonicerae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Flos Carthami into frying pan, add 8 times of decoctings again to endure, first seethe with excitement and decoct 2-3 hour at slow fire, stop adding when medicine soup quality is 1.3 times of medical material gross mass, medicinal residues are leached, obtains medicine soup;
Step 2
The spray-dried machine of medicine soup that step one is obtained, removing moisture obtains medicated powder;
Step 3
Gelatin is melted, and keeps temperature, make it maintain melting state for subsequent use;
Step 4
Aloe gel is poured in blender, and Aloe gel is heated to 52.3 degrees Celsius, and maintain this temperature, then the melted gelatin of medicated powder step 2 obtained and step 3 acquisition adds in Aloe gel, fully stirs and obtains mixed solution;
Step 5, molding
Before mixed solution step 4 obtained is to be cooled, pours mould into and make stype;
Step 4, packaging
Cooled stype is poured out mould, and load in special syringe, pack, cold preservation is for subsequent use.
Extracorporeal bacteria inhibitor test test objective of the present invention: adopt certain culture medium to cultivate the vaginal bacteria be recently separated or mycete, observe In Vitro Bacteriostasis of the present invention, and measure its minimum antibacterial crude drug consumption concentration.1, test material 1) bacterial strain reference culture: staphylococcus aureus (ATCC25923), escherichia coli (441490), beta hemolytic streptococcus (32210) are by, Candida albicans (98100).
2) instrument and equipment electronic balance, Beijing Sai Duolisi balance company limited produces; Full-automatic high-pressure autoclave, Japanese SANYO; Superclean bench, Suzhou Decontamination Equipment Plant; SHP-80 biochemical cultivation case, Shanghai is gloomy reliablely tests Instrument Ltd. 2 experimental technique 1) antibacterial culturing gets reference culture: staphylococcus aureus (ATCC25923), escherichia coli (441490), beta hemolytic streptococcus (32210) bacterial strain are a little, be inoculated in respectively in nutrient broth medium, cultivate 18h for 37 DEG C.(adding 20% calf serum in group B streptococcus broth bouillon) gets each bacterial strain nutrient broth culture that 18h cultivates, and does 10-3 dilution for experiment with nutrient broth.Extracting waste candidiasis bacterial strain is a little, is inoculated in improvement Martin culture medium, cultivates 24h for 35 DEG C.Get the Candida albicans culture that 24h cultivates, be used for experiment with improvement Martin culture medium 10-3 dilution.2) doubling dilution (test tube method) gets sterilizing test tubes 11,1st pipe adds nutrient broth fluid medium 1.6ml, all the other are pipe 1ml often, get respectively and add in the first pipe by reagent diluent 0.4ml, 1ml is got to the 2nd pipe after mixing, dilute successively, the 10th pipe sucking-off 1ml discards, and the 11st pipe does not add medicinal liquid in contrast.Often pipe adds dilution bacterium liquid 0.1ml, cultivates 24h for 37 DEG C.Take out and observe bacterial growth situation.As drug liquid tube is muddy, namely represent bacterial growth, for reagent thing without bacteriostasis; As drug liquid tube is limpid, represent that bacterial growth is suppressed, the medicinal liquid of the greatest dilution of its energy bacteria growing inhibiting, is the minimal inhibitory concentration (MIC) of this medicine.By limpid medicinal liquid meat soup each pipe transferred species broth agar plates, cultivate 24h and observe, be still the bacteriocidal concentration of this medicine without the minimum drug level of bacterial growth.Get sterilizing test tubes 11, the 1st pipe adds improvement Martin fluid medium 1.6ml, and all the other are pipe 1ml often, get respectively and add in the first pipe by reagent diluent 0.4ml, get 1ml after mixing to the 2nd pipe, dilute successively, 10th pipe sucking-off 1ml discards, and the 11st pipe does not add medicinal liquid in contrast.Often pipe adds dilution bacterium liquid 0.1ml, cultivates 24h for 35 DEG C, takes out and observe bacterial growth situation.As drug liquid tube is muddy, namely represent bacterial growth, for reagent thing without bacteriostasis; As drug liquid tube is limpid, represent that bacterial growth is suppressed, the medicinal liquid of the greatest dilution of its energy bacteria growing inhibiting, is the minimal inhibitory concentration (MIC) of this medicine.Limpid medicinal liquid meat soup each pipe transferred species sabouraud's agar is dull and stereotyped, cultivate 24h and observe, be still the bacteriocidal concentration of this medicine without the minimum drug level of bacterial growth.3, result experimental result display: the present invention is to reference culture: staphylococcus aureus (ATCC25923), escherichia coli (441490), beta hemolytic streptococcus (32210), Candida albicans all have stronger bacteriostasis.Bacterial strain minimum inhibitory concentration (MIC) (g crude drug/g) staphylococcus aureus (standard) 0.3 escherichia coli (standard) 0.3 group B streptococcus (standard) 0.2 Candida albicans 0.5.
4, be the curative effect investigating above-described embodiment medicine further, we have selected 100 vaginitis patients, verify the treatment curative effect of medicine of the present invention.
Be a component with every 50 people be 1 group and 2 groups, 2 groups is matched group, uses gynecological gel treatment.
| Grouping | Drag residence | Treatment time | Cure | Recurrence in half a year |
| 1 group | 3-5 hour | 3-5 days | 50 people | 1 people |
| 2 groups | 1-3 hour | 12-19 days | 50 people | 24 people |
embodiment 2
One treats gynecology colpitis hydrant reagent combination, is made up of following raw material: Indigo Naturalis 29 parts, Rhizoma Acori Graminei 24 parts, Semen Sterculiae Lychnophorae 19 parts, stir-baked SQUAMA MANITIS 13 parts, Flos Campsis 12 parts, Radix Ilicis Pubescentis 12 parts, the Radix Angelicae Dahuricae 9 parts, Semen Phaseoli 9 parts, Bombyx Batryticatus 6 parts, Radix Clematidis 6 parts, Herba Asari 8 parts, 7 parts, Moschus, xylitol 38 parts, gum base: 88 parts, Herba Menthae 19 parts.
Above-mentioned a kind of manufacture method for the treatment of gynecology colpitis hydrant reagent combination, comprises the following steps:
Step one, powder process
Pour Radix Stemonae, Flos Chrysanthemi Indici, Herba Violae, Folium Eucalypti Robustae, Rhizoma Corydalis, Indigo Naturalis, Camphora, Borneolum Syntheticum, Myrrha, Olibanum, catechu, Flos Lonicerae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Flos Carthami into frying pan, add 8 times of decoctings again to endure, first seethe with excitement and decoct 2-3 hour at slow fire, stop adding when medicine soup quality is 1.3 times of medical material gross mass, medicinal residues are leached, obtains medicine soup;
Step 2
The spray-dried machine of medicine soup that step one is obtained, removing moisture obtains medicated powder;
Step 3
Gelatin is melted, and keeps temperature, make it maintain melting state for subsequent use;
Step 4
Aloe gel is poured in blender, and Aloe gel is heated to 52.3 degrees Celsius, and maintain this temperature, then the melted gelatin of medicated powder step 2 obtained and step 3 acquisition adds in Aloe gel, fully stirs and obtains mixed solution;
Step 5, molding
Before mixed solution step 4 obtained is to be cooled, pours mould into and make stype;
Step 4, packaging
Cooled stype is poured out mould, and load in special syringe, pack, cold preservation is for subsequent use.
Extracorporeal bacteria inhibitor test test objective of the present invention: adopt certain culture medium to cultivate the vaginal bacteria be recently separated or mycete, observe In Vitro Bacteriostasis of the present invention, and measure its minimum antibacterial crude drug consumption concentration.1, test material 1) bacterial strain reference culture: staphylococcus aureus (ATCC25923), escherichia coli (441490), beta hemolytic streptococcus (32210) are by, Candida albicans (98100).
3) instrument and equipment electronic balance, Beijing Sai Duolisi balance company limited produces; Full-automatic high-pressure autoclave, Japanese SANYO; Superclean bench, Suzhou Decontamination Equipment Plant; SHP-80 biochemical cultivation case, Shanghai is gloomy reliablely tests Instrument Ltd. 2 experimental technique 1) antibacterial culturing gets reference culture: staphylococcus aureus (ATCC25923), escherichia coli (441490), beta hemolytic streptococcus (32210) bacterial strain are a little, be inoculated in respectively in nutrient broth medium, cultivate 18h for 37 DEG C.(adding 20% calf serum in group B streptococcus broth bouillon) gets each bacterial strain nutrient broth culture that 18h cultivates, and does 10-3 dilution for experiment with nutrient broth.Extracting waste candidiasis bacterial strain is a little, is inoculated in improvement Martin culture medium, cultivates 24h for 35 DEG C.Get the Candida albicans culture that 24h cultivates, be used for experiment with improvement Martin culture medium 10-3 dilution.2) doubling dilution (test tube method) gets sterilizing test tubes 11,1st pipe adds nutrient broth fluid medium 1.6ml, all the other are pipe 1ml often, get respectively and add in the first pipe by reagent diluent 0.4ml, 1ml is got to the 2nd pipe after mixing, dilute successively, the 10th pipe sucking-off 1ml discards, and the 11st pipe does not add medicinal liquid in contrast.Often pipe adds dilution bacterium liquid 0.1ml, cultivates 24h for 37 DEG C.Take out and observe bacterial growth situation.As drug liquid tube is muddy, namely represent bacterial growth, for reagent thing without bacteriostasis; As drug liquid tube is limpid, represent that bacterial growth is suppressed, the medicinal liquid of the greatest dilution of its energy bacteria growing inhibiting, is the minimal inhibitory concentration (MIC) of this medicine.By limpid medicinal liquid meat soup each pipe transferred species broth agar plates, cultivate 24h and observe, be still the bacteriocidal concentration of this medicine without the minimum drug level of bacterial growth.Get sterilizing test tubes 11, the 1st pipe adds improvement Martin fluid medium 1.6ml, and all the other are pipe 1ml often, get respectively and add in the first pipe by reagent diluent 0.4ml, get 1ml after mixing to the 2nd pipe, dilute successively, 10th pipe sucking-off 1ml discards, and the 11st pipe does not add medicinal liquid in contrast.Often pipe adds dilution bacterium liquid 0.1ml, cultivates 24h for 35 DEG C, takes out and observe bacterial growth situation.As drug liquid tube is muddy, namely represent bacterial growth, for reagent thing without bacteriostasis; As drug liquid tube is limpid, represent that bacterial growth is suppressed, the medicinal liquid of the greatest dilution of its energy bacteria growing inhibiting, is the minimal inhibitory concentration (MIC) of this medicine.Limpid medicinal liquid meat soup each pipe transferred species sabouraud's agar is dull and stereotyped, cultivate 24h and observe, be still the bacteriocidal concentration of this medicine without the minimum drug level of bacterial growth.3, result experimental result display: the present invention is to reference culture: staphylococcus aureus (ATCC25923), escherichia coli (441490), beta hemolytic streptococcus (32210), Candida albicans all have stronger bacteriostasis.Bacterial strain minimum inhibitory concentration (MIC) (g crude drug/g) staphylococcus aureus (standard) 0.3 escherichia coli (standard) 0.3 group B streptococcus (standard) 0.2 Candida albicans 0.5.
4, be the curative effect investigating above-described embodiment medicine further, we have selected 100 vaginitis patients, verify the treatment curative effect of medicine of the present invention.
Be a component with every 50 people be 1 group and 2 groups, 2 groups is matched group, uses gynecological gel treatment.
| Grouping | Drag residence | Treatment time | Cure | Recurrence in half a year |
| 1 group | 3-5 hour | 3-5 days | 50 people | 1 people |
| 2 groups | 1-3 hour | 12-19 days | 50 people | 24 people |
embodiment 3
One treats gynecology colpitis hydrant reagent combination, is made up of following raw material: Radix Stemonae 11.5 parts, Flos Chrysanthemi Indici 12.5 parts, Herba Violae 12.5 parts, Folium Eucalypti Robustae 13 parts, Rhizoma Corydalis 12 parts, Indigo Naturalis 8.5 parts, Camphora 9.5 parts, Borneolum Syntheticum 7.5 parts, Myrrha 23 parts, Olibanum 23 parts, 22.5 parts, catechu, Flos Lonicerae 22.5 parts, 22 parts, Radix Glycyrrhizae, Radix Angelicae Sinensis 11.5 parts, 11.5 parts, Flos Carthami, Aloe gel 48 parts, gel 52.5 parts.
Above-mentioned a kind of manufacture method for the treatment of gynecology colpitis hydrant reagent combination, comprises the following steps:
Step one, powder process
Pour Radix Stemonae, Flos Chrysanthemi Indici, Herba Violae, Folium Eucalypti Robustae, Rhizoma Corydalis, Indigo Naturalis, Camphora, Borneolum Syntheticum, Myrrha, Olibanum, catechu, Flos Lonicerae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Flos Carthami into frying pan, add 8 times of decoctings again to endure, first seethe with excitement and decoct 2-3 hour at slow fire, stop adding when medicine soup quality is 1.3 times of medical material gross mass, medicinal residues are leached, obtains medicine soup;
Step 2
The spray-dried machine of medicine soup that step one is obtained, removing moisture obtains medicated powder;
Step 3
Gelatin is melted, and keeps temperature, make it maintain melting state for subsequent use;
Step 4
Aloe gel is poured in blender, and Aloe gel is heated to 52.3 degrees Celsius, and maintain this temperature, then the melted gelatin of medicated powder step 2 obtained and step 3 acquisition adds in Aloe gel, fully stirs and obtains mixed solution;
Step 5, molding
Before mixed solution step 4 obtained is to be cooled, pours mould into and make stype;
Step 4, packaging
Cooled stype is poured out mould, and load in special syringe, pack, cold preservation is for subsequent use.
Extracorporeal bacteria inhibitor test test objective of the present invention: adopt certain culture medium to cultivate the vaginal bacteria be recently separated or mycete, observe In Vitro Bacteriostasis of the present invention, and measure its minimum antibacterial crude drug consumption concentration.1, test material 1) bacterial strain reference culture: staphylococcus aureus (ATCC25923), escherichia coli (441490), beta hemolytic streptococcus (32210) are by, Candida albicans (98100).
4) instrument and equipment electronic balance, Beijing Sai Duolisi balance company limited produces; Full-automatic high-pressure autoclave, Japanese SANYO; Superclean bench, Suzhou Decontamination Equipment Plant; SHP-80 biochemical cultivation case, Shanghai is gloomy reliablely tests Instrument Ltd. 2 experimental technique 1) antibacterial culturing gets reference culture: staphylococcus aureus (ATCC25923), escherichia coli (441490), beta hemolytic streptococcus (32210) bacterial strain are a little, be inoculated in respectively in nutrient broth medium, cultivate 18h for 37 DEG C.(adding 20% calf serum in group B streptococcus broth bouillon) gets each bacterial strain nutrient broth culture that 18h cultivates, and does 10-3 dilution for experiment with nutrient broth.Extracting waste candidiasis bacterial strain is a little, is inoculated in improvement Martin culture medium, cultivates 24h for 35 DEG C.Get the Candida albicans culture that 24h cultivates, be used for experiment with improvement Martin culture medium 10-3 dilution.2) doubling dilution (test tube method) gets sterilizing test tubes 11,1st pipe adds nutrient broth fluid medium 1.6ml, all the other are pipe 1ml often, get respectively and add in the first pipe by reagent diluent 0.4ml, 1ml is got to the 2nd pipe after mixing, dilute successively, the 10th pipe sucking-off 1ml discards, and the 11st pipe does not add medicinal liquid in contrast.Often pipe adds dilution bacterium liquid 0.1ml, cultivates 24h for 37 DEG C.Take out and observe bacterial growth situation.As drug liquid tube is muddy, namely represent bacterial growth, for reagent thing without bacteriostasis; As drug liquid tube is limpid, represent that bacterial growth is suppressed, the medicinal liquid of the greatest dilution of its energy bacteria growing inhibiting, is the minimal inhibitory concentration (MIC) of this medicine.By limpid medicinal liquid meat soup each pipe transferred species broth agar plates, cultivate 24h and observe, be still the bacteriocidal concentration of this medicine without the minimum drug level of bacterial growth.Get sterilizing test tubes 11, the 1st pipe adds improvement Martin fluid medium 1.6ml, and all the other are pipe 1ml often, get respectively and add in the first pipe by reagent diluent 0.4ml, get 1ml after mixing to the 2nd pipe, dilute successively, 10th pipe sucking-off 1ml discards, and the 11st pipe does not add medicinal liquid in contrast.Often pipe adds dilution bacterium liquid 0.1ml, cultivates 24h for 35 DEG C, takes out and observe bacterial growth situation.As drug liquid tube is muddy, namely represent bacterial growth, for reagent thing without bacteriostasis; As drug liquid tube is limpid, represent that bacterial growth is suppressed, the medicinal liquid of the greatest dilution of its energy bacteria growing inhibiting, is the minimal inhibitory concentration (MIC) of this medicine.Limpid medicinal liquid meat soup each pipe transferred species sabouraud's agar is dull and stereotyped, cultivate 24h and observe, be still the bacteriocidal concentration of this medicine without the minimum drug level of bacterial growth.3, result experimental result display: the present invention is to reference culture: staphylococcus aureus (ATCC25923), escherichia coli (441490), beta hemolytic streptococcus (32210), Candida albicans all have stronger bacteriostasis.Bacterial strain minimum inhibitory concentration (MIC) (g crude drug/g) staphylococcus aureus (standard) 0.3 escherichia coli (standard) 0.3 group B streptococcus (standard) 0.2 Candida albicans 0.5.
4, be the curative effect investigating above-described embodiment medicine further, we have selected 100 vaginitis patients, verify the treatment curative effect of medicine of the present invention.
Be a component with every 50 people be 1 group and 2 groups, 2 groups is matched group, uses gynecological gel treatment.
| Grouping | Drag residence | Treatment time | Cure | Recurrence in half a year |
| 1 group | 3-5 hour | 3-5 days | 50 people | 1 people |
| 2 groups | 1-3 hour | 12-19 days | 50 people | 24 people |
embodiment 4
One treats gynecology colpitis hydrant reagent combination, is made up of following raw material: Radix Stemonae 12.5 parts, Flos Chrysanthemi Indici 13.5 parts, Herba Violae 13.5 parts, Folium Eucalypti Robustae 14 parts, Rhizoma Corydalis 13 parts, Indigo Naturalis 9.5 parts, Camphora 10.5 parts, Borneolum Syntheticum 8.5 parts, Myrrha 24 parts, Olibanum 24 parts, 23.5 parts, catechu, Flos Lonicerae 23.5 parts, 23 parts, Radix Glycyrrhizae, Radix Angelicae Sinensis 12.5 parts, 12.5 parts, Flos Carthami, Aloe gel 49 parts, gel 53.5 parts.
Above-mentioned a kind of manufacture method for the treatment of gynecology colpitis hydrant reagent combination, comprises the following steps:
Step one, powder process
Pour Radix Stemonae, Flos Chrysanthemi Indici, Herba Violae, Folium Eucalypti Robustae, Rhizoma Corydalis, Indigo Naturalis, Camphora, Borneolum Syntheticum, Myrrha, Olibanum, catechu, Flos Lonicerae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Flos Carthami into frying pan, add 8 times of decoctings again to endure, first seethe with excitement and decoct 2-3 hour at slow fire, stop adding when medicine soup quality is 1.3 times of medical material gross mass, medicinal residues are leached, obtains medicine soup;
Step 2
The spray-dried machine of medicine soup that step one is obtained, removing moisture obtains medicated powder;
Step 3
Gelatin is melted, and keeps temperature, make it maintain melting state for subsequent use;
Step 4
Aloe gel is poured in blender, and Aloe gel is heated to 52.3 degrees Celsius, and maintain this temperature, then the melted gelatin of medicated powder step 2 obtained and step 3 acquisition adds in Aloe gel, fully stirs and obtains mixed solution;
Step 5, molding
Before mixed solution step 4 obtained is to be cooled, pours mould into and make stype;
Step 4, packaging
Cooled stype is poured out mould, and load in special syringe, pack, cold preservation is for subsequent use.
Extracorporeal bacteria inhibitor test test objective of the present invention: adopt certain culture medium to cultivate the vaginal bacteria be recently separated or mycete, observe In Vitro Bacteriostasis of the present invention, and measure its minimum antibacterial crude drug consumption concentration.1, test material 1) bacterial strain reference culture: staphylococcus aureus (ATCC25923), escherichia coli (441490), beta hemolytic streptococcus (32210) are by, Candida albicans (98100).
5) instrument and equipment electronic balance, Beijing Sai Duolisi balance company limited produces; Full-automatic high-pressure autoclave, Japanese SANYO; Superclean bench, Suzhou Decontamination Equipment Plant; SHP-80 biochemical cultivation case, Shanghai is gloomy reliablely tests Instrument Ltd. 2 experimental technique 1) antibacterial culturing gets reference culture: staphylococcus aureus (ATCC25923), escherichia coli (441490), beta hemolytic streptococcus (32210) bacterial strain are a little, be inoculated in respectively in nutrient broth medium, cultivate 18h for 37 DEG C.(adding 20% calf serum in group B streptococcus broth bouillon) gets each bacterial strain nutrient broth culture that 18h cultivates, and does 10-3 dilution for experiment with nutrient broth.Extracting waste candidiasis bacterial strain is a little, is inoculated in improvement Martin culture medium, cultivates 24h for 35 DEG C.Get the Candida albicans culture that 24h cultivates, be used for experiment with improvement Martin culture medium 10-3 dilution.2) doubling dilution (test tube method) gets sterilizing test tubes 11,1st pipe adds nutrient broth fluid medium 1.6ml, all the other are pipe 1ml often, get respectively and add in the first pipe by reagent diluent 0.4ml, 1ml is got to the 2nd pipe after mixing, dilute successively, the 10th pipe sucking-off 1ml discards, and the 11st pipe does not add medicinal liquid in contrast.Often pipe adds dilution bacterium liquid 0.1ml, cultivates 24h for 37 DEG C.Take out and observe bacterial growth situation.As drug liquid tube is muddy, namely represent bacterial growth, for reagent thing without bacteriostasis; As drug liquid tube is limpid, represent that bacterial growth is suppressed, the medicinal liquid of the greatest dilution of its energy bacteria growing inhibiting, is the minimal inhibitory concentration (MIC) of this medicine.By limpid medicinal liquid meat soup each pipe transferred species broth agar plates, cultivate 24h and observe, be still the bacteriocidal concentration of this medicine without the minimum drug level of bacterial growth.Get sterilizing test tubes 11, the 1st pipe adds improvement Martin fluid medium 1.6ml, and all the other are pipe 1ml often, get respectively and add in the first pipe by reagent diluent 0.4ml, get 1ml after mixing to the 2nd pipe, dilute successively, 10th pipe sucking-off 1ml discards, and the 11st pipe does not add medicinal liquid in contrast.Often pipe adds dilution bacterium liquid 0.1ml, cultivates 24h for 35 DEG C, takes out and observe bacterial growth situation.As drug liquid tube is muddy, namely represent bacterial growth, for reagent thing without bacteriostasis; As drug liquid tube is limpid, represent that bacterial growth is suppressed, the medicinal liquid of the greatest dilution of its energy bacteria growing inhibiting, is the minimal inhibitory concentration (MIC) of this medicine.Limpid medicinal liquid meat soup each pipe transferred species sabouraud's agar is dull and stereotyped, cultivate 24h and observe, be still the bacteriocidal concentration of this medicine without the minimum drug level of bacterial growth.3, result experimental result display: the present invention is to reference culture: staphylococcus aureus (ATCC25923), escherichia coli (441490), beta hemolytic streptococcus (32210), Candida albicans all have stronger bacteriostasis.Bacterial strain minimum inhibitory concentration (MIC) (g crude drug/g) staphylococcus aureus (standard) 0.3 escherichia coli (standard) 0.3 group B streptococcus (standard) 0.2 Candida albicans 0.5.
4, be the curative effect investigating above-described embodiment medicine further, we have selected 100 vaginitis patients, verify the treatment curative effect of medicine of the present invention.
Be a component with every 50 people be 1 group and 2 groups, 2 groups is matched group, uses gynecological gel treatment.
| Grouping | Drag residence | Treatment time | Cure | Recurrence in half a year |
| 1 group | 3-5 hour | 3-5 days | 50 people | 1 people |
| 2 groups | 1-3 hour | 12-19 days | 50 people | 24 people |
The equipment used in manufacture method of the present invention is the common equipment in pharmaceutical industries field, does not repeat them here.
The foregoing is only the schematic detailed description of the invention of the present invention, and be not used to limit scope of the present invention.Any those skilled in the art, equivalent variations done under the prerequisite not departing from design of the present invention and principle and amendment, all should belong to the scope of protection of the invention.
Claims (4)
1. a treatment gynecology colpitis hydrant reagent combination, it is characterized in that, be made up of the raw material of following weight portion: Radix Stemonae 10-13 part, Flos Chrysanthemi Indici 11-14 part, Herba Violae 11-14 part, Folium Eucalypti Robustae 11-15 part, Rhizoma Corydalis 10-14 part, Indigo Naturalis 7-10 part, Camphora 7-12 part, Borneolum Syntheticum 6-9 part, Myrrha 23-25 part, Olibanum 21-25 part, catechu 20-25 part, Flos Lonicerae 20-25 part, Radix Glycyrrhizae 20-24 part, Radix Angelicae Sinensis 10-13 part, Flos Carthami 10-13 part, aloe gel 40:1-56 parts, gel 47-68 part.
2. one as claimed in claim 1 treats gynecology colpitis hydrant reagent combination, it is characterized in that, be made up of the raw material of following weight portion: Radix Stemonae 11.5 parts, Flos Chrysanthemi Indici 12.5 parts, Herba Violae 12.5 parts, Folium Eucalypti Robustae 13 parts, Rhizoma Corydalis 12 parts, Indigo Naturalis 8.5 parts, Camphora 9.5 parts, Borneolum Syntheticum 7.5 parts, Myrrha 23 parts, Olibanum 23 parts, 22.5 parts, catechu, Flos Lonicerae 22.5 parts, 22 parts, Radix Glycyrrhizae, Radix Angelicae Sinensis 11.5 parts, 11.5 parts, Flos Carthami, Aloe gel 48 parts, gel 52.5 parts.
3. a kind of manufacture method for the treatment of gynecology colpitis hydrant reagent combination as claimed in claim 1 or 2, it is characterized in that, be made up of the raw material of following weight portion: Radix Stemonae 10-13 part, Flos Chrysanthemi Indici 11-14 part, Herba Violae 11-14 part, Folium Eucalypti Robustae 11-15 part, Rhizoma Corydalis 10-14 part, Indigo Naturalis 7-10 part, Camphora 7-12 part, Borneolum Syntheticum 6-9 part, Myrrha 23-25 part, Olibanum 21-25 part, catechu 20-25 part, Flos Lonicerae 20-25 part, Radix Glycyrrhizae 20-24 part, Radix Angelicae Sinensis 10-13 part, Flos Carthami 10-13 part, aloe gel 40:1-56 parts, gel 47-68 part.
4. comprise the following steps:
Step one, powder process
Pour Radix Stemonae, Flos Chrysanthemi Indici, Herba Violae, Folium Eucalypti Robustae, Rhizoma Corydalis, Indigo Naturalis, Camphora, Borneolum Syntheticum, Myrrha, Olibanum, catechu, Flos Lonicerae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Flos Carthami into frying pan, add 8 times of decoctings again to endure, first seethe with excitement and decoct 2-3 hour at slow fire, stop adding when medicine soup quality is 1.3 times of medical material gross mass, medicinal residues are leached, obtains medicine soup;
Step 2
The spray-dried machine of medicine soup that step one is obtained, removing moisture obtains medicated powder;
Step 3
Gelatin is melted, and keeps temperature, make it maintain melting state for subsequent use;
Step 4
Aloe gel is poured in blender, and Aloe gel is heated to 52.3 degrees Celsius, and maintain this temperature, then the melted gelatin of medicated powder step 2 obtained and step 3 acquisition adds in Aloe gel, fully stirs and obtains mixed solution;
Step 5, molding
Before mixed solution step 4 obtained is to be cooled, pours mould into and make stype;
Step 4, packaging
Cooled stype is poured out mould, and load in special syringe, pack, cold preservation is for subsequent use.
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Cited By (1)
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| CN106177270A (en) * | 2016-07-08 | 2016-12-07 | 张义淦 | A kind of vagina inhibiting-bacteria preparation and preparation method thereof |
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Application publication date: 20160113 |