CN105231020A - Feed additive composition and preparation method thereof - Google Patents
Feed additive composition and preparation method thereof Download PDFInfo
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- CN105231020A CN105231020A CN201510637006.4A CN201510637006A CN105231020A CN 105231020 A CN105231020 A CN 105231020A CN 201510637006 A CN201510637006 A CN 201510637006A CN 105231020 A CN105231020 A CN 105231020A
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Abstract
The invention relates to a feed additive composition and a preparation method thereof. The feed additive composition is composed of isomalto-oligosaccharide, fructo-oligosaccharide, radix astragali polysaccharide, bacillus subtilis, bacillus licheniformis, plant lactobacillus, lactobacillus acidophilus, enterococcus faecium, yeast-zinc, yeast-selenium, glucosylase, an anti-caking agent, and a carrier. The formula is scientific, the cost of signal component is high, the effect is bad, and the provided formula solves the problems. The cost is effectively reduced, while at the same time, an optimal effect is achieved. The composition can be added into feed to effectively exert the healthcare functions, can be used to replace antibiotics, and is safe to eat.
Description
Technical field
The present invention relates to a kind of feed addictive composition and preparation method thereof, belong to field of feed additive technology.
Background technology
Add antibacterials in feed be familiar with after coming out from 1940's antibiotic and use.Scientists finds, adds antibiotic or its fermentation residue, can promote growth of animals or poultry in feed.At the bottom of nineteen fifty, FDA (FDA) ratifies to add antibiotic first in feed, after, countries in the world have carried out antibiotic feeding experiment in succession, and for the production of.Although antibiotic has played huge effect in the infection of the price of deed and control animal that improve animal, but the abuse of antibiotic in aquaculture is also more and more serious, it is reported, China is the maximum antibiotics production state in the whole world and country of consumption, and the half nearly produced per year in 210,000 tons, antibiotic has been used in aquaculture aspect.The antibiotic discharge more than 50,000 tons in 1 year enters in soil and water resources.Pearl River Delta, Jing-jin-ji region Haihe basin are national antibiotic discharge intensity maximum regions, and in water, antibiotic concentration is very high.Yangtze River Delta Area because of discharge large, pollution is number three.It is seriously polluted that antibiotic is economized in Guangdong, Jiangsu, Zhejiang etc.
Except to environment seriously polluted except, a problem is also had to be exactly cause the health of persister to the mankind itself to cause serious threat because extensively adding antibiotic in feed, because routine use antibiotic in animal husbandry, the microorganism of carrying antibiotic resistance gene can be made to grow prosperity.In some cases, antibiotic resistance gene can become has very high mobility, and that is they can move to those and can go in the bacterium of human body cause illness.So, when human patients goes to take antibiotic again time, because bacterium has drug resistance, originally magical medicine just loses effectiveness, and various superbacteria is born in succession.Due to the microbial infection of resistance, antibiotic is uncontrollable, finally causes patient death.In the sixties in last century, the number that infectious diseases is died from the whole world is every year about 7,000,000, and this numeral rises to 2,000 ten thousand to the beginning of this century.The number dying from septicemia rises 89%, and most people die from the medication difficulty that superbacteria brings.
Just because of above reason, forbid in feed adding attention and the accreditation that antibiotic is just obtaining more and more national, European Union has completely forbidden and added antibiotic in feed, the U.S. also adds antibiotic kind and quantity in limiting feed, in view of the degree of abuse of antibiotics in China's feed is higher than the U.S. and European Union, therefore, for China, forbidding in feed that antibiotic adds also is trend of the times.
Summary of the invention
In order to overcome the above problems, the invention provides a kind of feed addictive composition and preparation method thereof.Antibiotic is replaced for adding in feed.
In order to realize object of the present invention, the present invention adopts following technical scheme:
A kind of feed addictive composition, it is characterized in that being turned glycosides enzyme, anti-caking agent and carrier formed by oligoisomaltose, FOS, astragalus polyose, bacillus subtilis, bacillus licheniformis, Lactobacillus plantarum, lactobacillus acidophilus, VREF, yeast zinc, ferment wood selenium, glucose
With parts by weight, oligoisomaltose 70-110 part, FOS 20-50 part, astragalus polyose 10-20 part, bacillus subtilis 1-3 part, bacillus licheniformis 1-3 part, Lactobacillus plantarum 1-2 part, lactobacillus acidophilus 1-2 part, VREF 1-2 part, yeast zinc 0.5-1 part, ferment wood selenium 0.5-1 part, glucose turn glycosides enzyme 0.25-0.5 part, anti-caking agent 0.5-1 part, carrier 150-200 part.
Described anti-caking agent refer to calcium silicates, calcium stearate, silica, bentonite,
One or more in diatomite, kaolin;
Described carrier refers to one or more in maize cob meal, corn flour, wheat bran, defatted rice bran, corn stalk powder, powdered rice hulls;
The weight ratio of its preferred each raw material is:
Oligoisomaltose 90 parts, FOS 30 parts, astragalus polyose 15 parts, bacillus subtilis 2 parts, bacillus licheniformis 2 parts, Lactobacillus plantarum 1 part, lactobacillus acidophilus 1 part, VREF 1 part, yeast zinc 0.75 part, ferment wood 0.5 part, selenium, anti-caking agent 0.5 part, glucose turn glycosides enzyme 0.35 part, 200 parts, carrier.
In composition of raw materials, the performance of each component is:
Oligoisomaltose, FOS: in present feed, add the biological oligosaccharides of the most extensive, that effect cost performance is the highest two classes, promote the increment of beneficial bacterium in enteron aisle, reduce enteron aisle pH value, suppress the growth of pathogenic bacteria in enteron aisle, improve body's immunity, prevention is infected, improve digestive function and regularity, prevent constipation from suffering from diarrhoea, promote the absorption of mineral matter, alleviate the burden of kidney.
Astragalus polyose: can be used as immunopotentiating agent or conditioning agent, has antiviral, antitumor, anti-ageing, radioresistance, resisting stress, the effect such as anti-oxidant simultaneously.
Bacillus subtilis, bacillus licheniformis: have outstanding biology and take oxygen ability by force, consume rapidly the free oxygen in environment, cause enteron aisle hypoxemia, promote that useful anaerobic bacteria grows, and produce the organic acids such as lactic acid, reduce enteron aisle pH value, indirectly suppress the growth of other pathogenic bacteria.The enzymes such as synthesis AMS, protease, lipase, cellulase, together play a role with the digestive enzymes in animal body in alimentary canal.The multiple B family vitamins such as synthetic vitamin B1, B2, B6, nicotinic acid, improve the activity of interferon and macrophage in animal body.
Lactobacillus plantarum, lactobacillus acidophilus, VREF: three is the endogenous probio existed in animal intestinal, after being separated from the enteron aisle of animal by the present inventor, optimize through constantly, screen, be separated and obtain, entering after the enteron aisle of animal can field planting rapidly, ferment rapidly in enteron aisle, produce the small molecular organic acids such as lactic acid, the material of other growth of probiotics such as releasing beneficial Bifidobacterium, increase the quantity of probio in large intestine, produce vitamin K and Cobastab, the utilization rate of calcium, phosphorus, iron can be improved, promote the absorption of iron and vitamin D.
Yeast zinc, ferment wood selenium: selenium, zinc are all one of trace elements necessary in animal body.Selenium has anti-oxidant, to improve immunologic function effect.In organism, the content of zinc accounts for second in trace element, and the activity of nearly more than 300 kinds of enzymes is relevant with zinc in six large enzyme systems.It carries the different biological function of kind more than 20, almost relates to the metabolic various aspects of organism.This patent yeast zinc, selenium belong to the biological zinc of the third generation, the representative of selenium, by yeast, zinc, selenium are absorbed and transformed in growth course, zinc selenium is combined with Yeast protein and polysaccharide, form zinc, the selenium of pure biological state, and provide sufficient carrier for the absorption of zinc, selenium, stimulate without toxicity and stomach, bioavilability is up to more than 80% simultaneously.Rich zinc, selenium yeast are rich in polysaccharide, B family vitamin, dietary fiber and organic mineral substance simultaneously.Animal body supplements multidimensional natural nutrient while supplementary biological zinc, selenium element, comprehensive nutrition, equilibrium, complete, improves immunity of organisms, meets the physiological requirements that organism is multi-faceted.
The preparation method of above-mentioned a kind of feed addictive composition, after it is characterized in that selenium, anti-caking agent and carrier fully mix by required weight fraction by yeast zinc, ferment wood, add the oligoisomaltose of required weight fraction more successively, FOS, astragalus polyose fully mix, last adding successively needs the bacillus subtilis of weight fraction, bacillus licheniformis, Lactobacillus plantarum, lactobacillus acidophilus, VREF and glucose to turn glycosides enzyme fully to mix, lucifuge drying preservation, measures according to actual needs during use to add in feed to mix thoroughly and processes.
Beneficial effect of the present invention:
1. the present invention functional sugar oligoisomaltose, FOS and astragalus polyose and bacteria useful to microecology bacillus subtilis, bacillus licheniformis, Lactobacillus plantarum, lactobacillus acidophilus, VREF and yeast zinc, yeast selenium is reasonably combined together with, scientific composition, efficiently solve single component cost high, the problem of weak effect, under the prerequisite effectively reduced costs, achieve the maximization of function, be added in feed, can effectively play its health care, replace antibiotic, ensure food safety.
2. glucose turns glycometabolism approach and cell intracellular metabolic in glycosides enzyme participation animal body, damaged cell can be repaired, oligoisomaltose, FOS and thalline broken wall product can be enteron aisle, particularly hind microflora provides nutriment, its amount reproduction short, secrete a large amount of digestive ferments and immune factor, thus improve digestive utilization ratio and the premunition of feed.
3. the present invention is by the use of carrier and anti-agglomerating agent, efficiently solve the problem of oligoisomaltose and the easy deliquescence caking of FOS, simultaneously because oligoisomaltose, FOS all have good proliferation function to lactobacillus acidophilus, lactobacillus lactis, streptococcus lactis, bacillus subtilis, bacillus licheniformis, effectively mixed by this, also solve Tiny ecosystem probio be difficult to ensure deposit, the problem of easy in inactivation.
4. detailed description of the invention
embodiment 1
A kind of feed addictive composition, with parts by weight, turn glycosides enzyme 0.25 part, 150 parts, carrier containing oligoisomaltose 70 parts, FOS 20 parts, astragalus polyose 20 parts, bacillus subtilis 1 part, bacillus licheniformis 1 part, Lactobacillus plantarum 1 part, lactobacillus acidophilus 1 part, VREF 1 part, yeast zinc 0.5 part, ferment wood 0.5 part, selenium, anti-caking agent 0.5 part, glucose.
Described anti-caking agent refers to calcium silicates;
Described carrier refers to maize cob meal;
The preparation method of feed addictive composition: after yeast zinc 0.5 part, ferment wood 0.5 part, selenium, calcium silicates 0.5 part are fully mixed with maize cob meal 150 parts, add the fully mixing of oligoisomaltose 70 parts, FOS 20 parts, astragalus polyose 20 parts more successively, last add bacillus subtilis 1 part successively, bacillus licheniformis 1 part, Lactobacillus plantarum 1 part, lactobacillus acidophilus 1 part, VREF 1 part, glucose turns glycosides enzyme 0.25 part fully mixing, lucifuge drying preservation, measures according to actual needs during use to add in feed to mix thoroughly and processes.
embodiment 2
A kind of feed addictive composition, with parts by weight, turn glycosides enzyme 0.35 part, anti-caking agent 0.75 part, 175 parts, carrier containing oligoisomaltose 90 parts, FOS 30 parts, astragalus polyose 15 parts, bacillus subtilis 2 parts, bacillus licheniformis 2 parts, Lactobacillus plantarum 1.5 parts, lactobacillus acidophilus 1.5 parts, VREF 1.5 parts, yeast zinc 0.75 part, ferment wood 0.75 part, selenium, glucose.
Described anti-caking agent refers to silica;
Described carrier refers to wheat bran;
The preparation method of feed addictive composition: after yeast zinc 0.75 part, ferment wood 0.75 part, selenium, silica 0.75 part are fully mixed with 175 parts, wheat bran, add the fully mixing of oligoisomaltose 90 parts, FOS 30 parts, astragalus polyose 15 parts more successively, last add bacillus subtilis 2 parts successively, bacillus licheniformis 2 parts, Lactobacillus plantarum 1.5 parts, lactobacillus acidophilus 1.5 parts, VREF 1.5 parts, glucose turns glycosides enzyme 0.35 part fully mixing, lucifuge drying preservation, measures according to actual needs during use to add in feed to mix thoroughly and processes.
embodiment 3
A kind of feed addictive composition, with parts by weight, turn glycosides enzyme 0.5 part, anti-caking agent 1 part, 200 parts, carrier containing oligoisomaltose 110 parts, FOS 50 parts, astragalus polyose 20 parts, bacillus subtilis 3 parts, bacillus licheniformis 3 parts, Lactobacillus plantarum 2 parts, lactobacillus acidophilus 2 parts, VREF 2 parts, yeast zinc 1 part, ferment wood 1 part, selenium, glucose.
Described anti-caking agent refers to diatomite;
Described carrier refers to defatted rice bran;
The preparation method of feed addictive composition: after yeast zinc 1 part, ferment wood 1 part, selenium, 1 part, diatomite are fully mixed with defatted rice bran 200 parts, add the fully mixing of oligoisomaltose 110 parts, FOS 50 parts, astragalus polyose 20 parts more successively, last add bacillus subtilis 3 parts successively, bacillus licheniformis 3 parts, Lactobacillus plantarum 2 parts, lactobacillus acidophilus 2 parts, VREF 2 parts, glucose turns glycosides enzyme 0.5 part fully mixing, lucifuge drying preservation, measures according to actual needs during use to add in feed to mix thoroughly and processes.
Experimental example 1
Test is divided into 4 process, the grower pigs of each processing selecting weight average 6, if 3 repetitions, each repetition 2, totally 24 pigs carry out digestion trial (see table 1).Group 1 is control group, and group 2 is antibiotic positive controls, adds the fortimicin of 200mg/kg, group 3,4 is test group, and add compositions of additives described in 500mg/kg and 1000mg/kg embodiment 2 respectively, experiment effect is in table 1.
Table 1 embodiment 2 is on the impact of grower pigs plant recovery of nutrient
Note: different not significantly (P>0.05) with column data shoulder mark identical table differential, different expression significant difference (P<0.05).
As shown in Table 1, the Apparent digestive utilization ratio of the thick protein in test group feed, crude fat, crude fibre, coarse ash be all significantly higher than blank group (group 1) (
p<0.05), but between experimental group without significant difference (
p>0.05).Group 3, organize 4 crude fibre apparent digestibilities be significantly higher than group 1, group 2.
experimental example 2
Two tests all choose 40 week age, the laying hen 5000 that body condition is close, and be divided into two groups at random, be respectively test group and control group, often group establishes two repetitions.Control group select in basal diet add 150mg the aureomycin of kg, test group selects embodiment 2 compositions of additives adding 1000mg/kg in test daily ration.Basal diet is prepared with reference to egg fowl feeding standard.Experiment effect is in table 2.
Table 2 embodiment 2 compositions of additives is on the impact of performance in layers and Egg Quality
| Laying rate (%) | Feedstuff-egg ratio | Hatchable egg rate (%) | Broken soft egg rate (%) | |
| Control group | 87.08±1.21 a | 2.33 | 92.11±0.98 A | 1.47±0.41 a |
| Test group | 88.21±0.92 b | 2.11 | 92.84±0.87 B | 0.98±0.23 b |
27.
note:same column shoulder mark capitalization difference expression significant difference (
p<0.05), same column shoulder mark lowercase difference represents that difference is extremely remarkable
(p<0.01).
As shown in Table 2: control group laying rate is inequality heteropole remarkable (p<0.01) compared with test group, control group hatchable egg rate is equal significant difference (p<0.05) compared with test group, control group breaks soft egg rate difference extremely remarkable (p<0.01) compared with test group, feedstuff-egg ratio reduces by 9.4%, and difference is extremely remarkable.
By table 2, the raising 1.29% compared with control group of test group laying breed laying rate, feedstuff-egg ratio reduces by 9.4%, and hatchable egg rate improves 0.79%, and broken soft egg rate reduces by 4.12%.
experimental example 3
Select 25 age in days weanling pig 300, be divided into 2 groups at random.Be respectively antibiotic group and experimental group, compositions of additives described in the present embodiment 2 of experimental group interpolation 0.2%, antibiotic group adds aureomycin 75ppm, Avilamycin 20ppm(in sterling).Experiment periods 40 days, experimental result is as follows:
Table 3 embodiment 2 compositions of additives is on the impact of weaned piglets
| Group | Antibiotic group | Experimental group |
| Average daily gain (g) | 384 | 395 |
| Average daily ingestion amount (g) | 565 | 560 |
| Feed conversion rate | 1.46 | 1.40 |
| Diarrhoea head number | 8 | 2 |
As shown in Table 3: after adding compositions of additives described in 0.2% the present embodiment 2 in weanling pig (21-61 age in days) daily ration, compared with antibiotic group, daily gain improves 2.9%, and feed-weight ratio improves 4.3%, and significantly can reduce the diarrhea rate of sucking pig.
Table 4 embodiment 2 compositions of additives is on the impact of piglet immunological globulin
| Group | Antibiotic group | Experimental group |
| lgG | 2.55 | 2.90 |
| lgA | 1.15 | 1.25 |
| lgM | 0.25 | 0.35 |
As shown in Table 4: described in the present embodiment 2 of interpolation 0.2% in daily ration, compositions of additives is to piglet immunological globulin lgG, lgA, lgM effect of increasing significantly, and illustrates in daily ration and adds the immunity that compositions of additives described in this patent can improve piglet.
Table 5 embodiment 2 compositions of additives is on the impact of Serum Biochemical Index in Piglets
| Group | Antibiotic group | Experimental group |
| Glutamic-pyruvic transaminase (ALT, U/L) | 27.33 | 28.10 |
| Glutamic-oxalacetic transaminease (AST, U/L) | 46.77 | 48.02 |
| Alkaline phosphatase (ALP, U/L) | 148.7 | 149.1 |
| Glucose (GLU, mmol/L) | 6.09 | 6.12 |
| T-CHOL (TC, mmol/L) | 2.43 | 2.44 |
As shown in Table 5: described in the present embodiment 2 of interpolation 0.2% in daily ration, compositions of additives can significantly improve the content of piglet serum glutamic pyruvic transminase, glutamic-oxalacetic transaminease, alkaline phosphatase, illustrates in daily ration that adding FOS effectively can improve the anabolism of protein and the deposition of calcium phosphorus.
. table 6 embodiment 2 compositions of additives is on the impact (unit is: lgcfu/g) of intestine of young pigs flora
| Group | Antibiotic group | Experimental group |
| Duodenum | ||
| Lactic acid bacteria | 5.03 | 5.83 |
| Escherichia coli | 1.13 | 1.07 |
| Jejunum | ||
| Lactic acid bacteria | 3.65 | 4.54 |
| Escherichia coli | 3.34 | 3.03 |
| Ileum | ||
| Lactic acid bacteria | 4.03 | 4.74 |
| Escherichia coli | 0.78 | 0.55 |
As shown in Table 6: described in the present embodiment 2 of interpolation 0.2% in daily ration, compositions of additives can significantly improve intestine of young pigs lactic acid bacterium number, reduce Escherichia coli quantity, illustrate that it can play the effect regulating intestine of young pigs colony balance.
From above experimental example, this feed addictive composition can improve the digestibility of animal significantly, improves the general level of the health of animal, improves the production performance of animal, can the use of substitute antibiotics effectively, is worth vigorously promoting the use at feedstuff industry.
Claims (5)
1. a feed addictive composition, it is characterized in that by oligoisomaltose, FOS, astragalus polyose, bacillus subtilis, bacillus licheniformis, Lactobacillus plantarum, lactobacillus acidophilus, VREF, yeast zinc, ferment wood selenium, glucose turns glycosides enzyme, anti-caking agent and carrier composition, with parts by weight, oligoisomaltose 70-110 part, FOS 20-50 part, astragalus polyose 10-20 part, bacillus subtilis 1-3 part, bacillus licheniformis 1-3 part, Lactobacillus plantarum 1-2 part, lactobacillus acidophilus 1-2 part, VREF 1-2 part, yeast zinc 0.5-1 part, ferment wood selenium 0.5-1 part, glucose turns glycosides enzyme 0.25-0.5 part, anti-caking agent 0.5-1 part, carrier 150-200 part.
2. described a kind of feed addictive composition as claimed in claim 1, it is characterized in that anti-caking agent refer in calcium silicates, calcium stearate, silica, bentonite, diatomite, kaolin one or more.
3. described a kind of feed addictive composition as claimed in claim 1, is characterized in that one or more that described carrier refers in maize cob meal, corn flour, wheat bran, defatted rice bran, corn stalk powder, powdered rice hulls.
4. described a kind of feed addictive composition as claimed in claim 1, the weight ratio of its preferred each raw material is: oligoisomaltose 90 parts, FOS 30 parts, astragalus polyose 15 parts, bacillus subtilis 2 parts, bacillus licheniformis 2 parts, Lactobacillus plantarum 1 part, lactobacillus acidophilus 1 part, VREF 1 part, yeast zinc 0.75 part, ferment wood 0.5 part, selenium, anti-caking agent 0.5 part, glucose turn glycosides enzyme 0.35 part, 200 parts, carrier.
5. the preparation method of feed addictive composition as claimed in claim 1, after it is characterized in that selenium, anti-caking agent and carrier fully mix by required weight fraction by yeast zinc, ferment wood, add the oligoisomaltose of required weight fraction more successively, FOS, astragalus polyose fully mix, last adding successively needs the bacillus subtilis of weight fraction, bacillus licheniformis, Lactobacillus plantarum, lactobacillus acidophilus, VREF and glucose to turn glycosides enzyme fully to mix, lucifuge drying preservation, measures according to actual needs during use to add in feed to mix thoroughly and processes.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106173383A (en) * | 2016-07-08 | 2016-12-07 | 江苏星海生物科技有限公司 | A kind of children's pig feed additive and preparation method |
| CN107136303A (en) * | 2017-05-23 | 2017-09-08 | 吉林远通矿业有限公司 | A kind of modification infusorial earth probiotics and preparation method thereof |
| CN107836585A (en) * | 2017-12-26 | 2018-03-27 | 湖南农业大学 | A kind of feed addictive for improving sow milk performance and oxidation resistance and preparation method thereof |
| CN111631317A (en) * | 2019-03-01 | 2020-09-08 | 张德珍 | Animal nutrient solution containing nano-selenium compound bacterium enzyme |
| CN115316566A (en) * | 2022-08-30 | 2022-11-11 | 中国水产科学研究院黑龙江水产研究所 | Additive for carp feed and use method of additive |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106173383A (en) * | 2016-07-08 | 2016-12-07 | 江苏星海生物科技有限公司 | A kind of children's pig feed additive and preparation method |
| CN107136303A (en) * | 2017-05-23 | 2017-09-08 | 吉林远通矿业有限公司 | A kind of modification infusorial earth probiotics and preparation method thereof |
| CN107836585A (en) * | 2017-12-26 | 2018-03-27 | 湖南农业大学 | A kind of feed addictive for improving sow milk performance and oxidation resistance and preparation method thereof |
| CN111631317A (en) * | 2019-03-01 | 2020-09-08 | 张德珍 | Animal nutrient solution containing nano-selenium compound bacterium enzyme |
| CN115316566A (en) * | 2022-08-30 | 2022-11-11 | 中国水产科学研究院黑龙江水产研究所 | Additive for carp feed and use method of additive |
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