CN105213530A - A kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract and its preparation method and application - Google Patents
A kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract and its preparation method and application Download PDFInfo
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Abstract
Disclosed by the invention is a kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract and its preparation method and application.Described Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are for raw material is prepared from Ampelopteris prolifera (Retz.) Copel. of 1:8 ~ 8:1 weight ratio and Radix rubi buergeri; Mix according to a certain weight ratio with cyclophosphamide with Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, or, mix according to a certain weight ratio with pemetrexed with Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the application in preparation anti-gastric cancer, esophageal carcinoma, colon cancer or rectal cancer medicine.Ampelopteris prolifera (Retz.) Copel. of the present invention and Radix rubi buergeri composite extract have certain active anticancer, with cyclophosphamide or pemetrexed, there is synergism, can greatly improve its anti-tumor activity, and safe and effective, control recurrence rate after healing low, for antitumor drug provides a kind of selection newly; Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract preparation method simply, are easy to operation, and preparation amount is large, and product yield is high.
Description
Technical field
The present invention relates to technical field, specifically relate to a kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract and its preparation method and application.
Background technology
Ampelopteris prolifera (Retz.) Copel. is the herb on Thelypteridaceae plant Ampelopteris prolifera (Retz.) Copel..The form on plant Ampelopteris prolifera (Retz.) Copel. is: plant climbs up by holding on to, high 50-170cm.Rhizome grows wild, wooden, and dredge by yellowish-brown, lanceolar scale, the short point in top, Quan Yuan, there is limb ray cilium outside.Leafage life or closely life; The long 15-20cm of petiole, straw color, glossy, base portion burgundy and slightly expanding, dredges by scale, to Smooth, has shallow ridges and rib above with rachis; The hard herbaceous stem of blade or papery, the wealthy lanceolar of oval shape, long 30-150cm or longer, wide 10-20cm, a pinniform, blade is indeterminate growth, namely bears bulbil between accessory pinna axil, thus branch, these branch bodies are less and do not give birth to sporangiorus, the tip of rachis lands and takes root, and forms new plant, row asexual propagation; Blade is near without hair or there is canescence needle-like hair below; There is undercoat fossula both sides above rachis and pinna rachis; Swell below pinna rachis and middle arteries and veins; Vein pinniform, lateral vein 6-9 couple, top 2-3 are to being separated and stretching Da Yebian, and remaining connects into rhomboid mesh, and layman's scun is tortuous.Be circular or Long Circle during sporangiorus children, carry on the back and be born in the middle part of lateral vein, respectively line up 1 row along Zhong Mai both sides, maturation is often converged and is abound with in the accessory pinna back side; Without indusium.Be born in the Yan Xi limit, river of height above sea level 100-1000m, often climb up by holding on in thick forest or bushes.Be distributed in south China, southwest and the ground such as Fujian, Taiwan.Medical material begins to be loaded in " Chinese main plant figure says Pteridophyta " Ampelopteris prolifera (Retz.) Copel..Acrid in the mouth, cool in naturely returns Liver Channel, stomach warp.Heat clearing away, dampness removing, main gastritis, dysentery, stranguria with turbid discharge, rheumatism swells and ache.
Radix rubi buergeri is that rosaceous plant is trembled with fear the root of the certain kind of berries.The tremble with fear form of the certain kind of berries of plant is: climing property evergreen dwarf shrub.Stem often throws oneself on the ground to take root, and grows new strain, close raw brown or canescence pubescence, stingless or have minority to sting; Branch of crawling reaches 2m.Dan Ye; The long 3-9cm of petiole; Stipule lacinia; Blade subcircular, diameter 5-11cm, the anxious point of tip or circle blunt, base portion is heart-shaped, and edge normal 5 shallowly splits, above near without hair, below with petiole villous, closeer along vein.Raceme is short, and axil is raw, has colored 4-10 piece, intensive; Total bennet and the close raw canescence down of bennet and scattered thorn bristle; Floral white, diameter is about 1cm; Calyx lobe lanceolar, outside is faint yellow becomes mildewed.Aggregate fruit is subsphaeroidal, diameter 6-10mm, atropurpureus.The florescence 7-8 month, the fruit phase 9-10 month.Medical material Radix rubi buergeri is recorded according to " the south of Fujian Province traditional herbal medicine ": sour in the mouth, and property is put down, nontoxic.Jiangxi " medical herbs handbook ": promoting blood circulation and cooling blood, heat-clearing and toxic substances removing.Control icterohepatitis, menoxenia, puerperal fever, children's's hyperpyrexia, hemorrhoid perianal hemorroids." practical Chinese herbal medicine ": convergence, removing toxic substances, pain relieving.Treat for stomachache, hyperchlorhydria, seminal emission, headache.
Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri, the current traditional Chinese medical science seldom uses, especially very few in the research of antitumor this respect.And in recent years, China's cancer morbidity is in rising trend, the research of various anti-cancer and cancer-preventing health food becomes current study hotspot.Our research show Ampelopteris prolifera (Retz.) Copel. extract and Radix rubi buergeri extract there is certain active anticancer, and with cyclophosphamide or pemetrexed, there is synergism, can greatly improve its anti-tumor activity.Not yet report that these two kinds of extract combination are reported in the research of anti-tumor aspect at present, also have no the report of these two kinds of extract preparation technologies and Patents.
Summary of the invention
The technical problem that the present invention solves is to provide a kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract.
The present invention also provides the preparation method of a kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract.
The present invention also provides the application of a kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract.
The present invention is achieved through the following technical solutions:
A kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are for raw material is prepared from Ampelopteris prolifera (Retz.) Copel. of 1:8 ~ 8:1 weight ratio and Radix rubi buergeri.
A kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are prepared from according to the method comprised the steps:
1) take Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri by described proportioning, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri are washed respectively, after drying with microwave Chinese herbal medicine drying equipment, mixing, be ground into 60 order coarse powder, mix with the part by weight of deionized water by 1:5.1 ~ 1:5.5, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry;
2) in step 1) add in gained Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry 0.02 ~ 0.2% antifreeze, regulate its temperature to-20 ~-14 DEG C, 10 ~ 20h is kept under this temperature conditions, then heat up with the intensification of 0.1 ~ 0.3 DEG C speed, be warming up to 0 DEG C, 15 ~ 25h is kept under this temperature conditions, then heat up with the intensification of 0.3 ~ 0.5 DEG C speed, be warming up to 46 ~ 50 DEG C, 5 ~ 10h is kept under this temperature conditions, send into Chinese-medicinal thermal-refluxing extraction group, utilize negative pressure of vacuum to carry out extract at low temperature and cryoconcentration, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract;
Further, the process conditions of described extraction and concentration are: the operating temperature in extraction pot is 68 ~ 70 DEG C, extraction time is 1.2 ~ 1.5h, thickening temperature controls at 62 ~ 65 DEG C, be concentrated into crystallization to separate out completely, the operating pressure of extraction pot is 0.93MPa, maximumly maintains Chinese crude drug active ingredient not by drain evaporation.
Further, described antifreeze is methanol or ethanol.
A kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are preparing the application in antitumor drug.
Further, described tumor is gastric cancer, esophageal carcinoma, colon cancer or rectal cancer.
Further, be mix according to a certain weight ratio with cyclophosphamide with Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparing the application in antitumor drug.
Further, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract and cyclophosphamide by weight proportion be 17:1 ~ 28:1.
Or, be mix according to a certain weight ratio with pemetrexed with Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparing the application in antitumor drug.
Further, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract and pemetrexed by weight proportion be 13:1 ~ 22:1.
The invention has the beneficial effects as follows: Ampelopteris prolifera (Retz.) Copel. of the present invention and Radix rubi buergeri composite extract have certain active anticancer, with cyclophosphamide or pemetrexed, there is synergism, can greatly improve its anti-tumor activity, and it is safe and effective, control recurrence rate after healing low, for antitumor drug provides a kind of selection newly; Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract preparation method simply, are easy to operation, and preparation amount is large, and product yield is high.
Detailed description of the invention
Embodiment 1: a kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract is for raw material is prepared from Ampelopteris prolifera (Retz.) Copel. of 1:8 weight ratio and Radix rubi buergeri.
This Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparation method is as follows:
1) take Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri by described proportioning, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri are washed respectively, after drying with microwave Chinese herbal medicine drying equipment, mixing, be ground into 60 order coarse powder, mix with the part by weight of deionized water by 1:5.1, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry;
2) in step 1) add in gained Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry 0.02% antifreeze, described antifreeze is methanol, regulate its temperature to-20 DEG C, 10h is kept under this temperature conditions, then heat up with the intensification of 0.1 DEG C speed, be warming up to 0 DEG C, 15h is kept under this temperature conditions, then heat up with the intensification of 0.3 DEG C speed, be warming up to 46 DEG C, 5h is kept under this temperature conditions, send into Chinese-medicinal thermal-refluxing extraction group, negative pressure of vacuum is utilized to carry out extract at low temperature and cryoconcentration, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the process conditions of described extraction and concentration are: the operating temperature in extraction pot is 68 DEG C, extraction time is 1.2h, thickening temperature controls at 62 DEG C, be concentrated into crystallization to separate out completely, the operating pressure of extraction pot is 0.93MPa, maximumly maintain Chinese crude drug active ingredient not by drain evaporation.
This Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are for the preparation of anti-gastric cancer medicament.
Embodiment 2:
A kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are for raw material is prepared from Ampelopteris prolifera (Retz.) Copel. of 1:1 weight ratio and Radix rubi buergeri.
This Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparation method is as follows:
1) take Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri by described proportioning, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri are washed respectively, after drying with microwave Chinese herbal medicine drying equipment, mixing, be ground into 60 order coarse powder, mix with the part by weight of deionized water by 1:5.3, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry;
2) in step 1) add in gained Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry 0.011% antifreeze, described antifreeze is ethanol, regulate its temperature to-17 DEG C, 15h is kept under this temperature conditions, then heat up with the intensification of 0.2 DEG C speed, be warming up to 0 DEG C, 20h is kept under this temperature conditions, then heat up with the intensification of 0.4 DEG C speed, be warming up to 48 DEG C, 7.5h is kept under this temperature conditions, send into Chinese-medicinal thermal-refluxing extraction group, negative pressure of vacuum is utilized to carry out extract at low temperature and cryoconcentration, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the process conditions of described extraction and concentration are: the operating temperature in extraction pot is 69 DEG C, extraction time is 1.35h, thickening temperature controls at 63.5 DEG C, be concentrated into crystallization to separate out completely, the operating pressure of extraction pot is 0.93MPa, maximumly maintain Chinese crude drug active ingredient not by drain evaporation.
This Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are for the preparation of anti-esophageal cancer medicine.
Embodiment 3:
A kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are for raw material is prepared from Ampelopteris prolifera (Retz.) Copel. of 8:1 weight ratio and Radix rubi buergeri.
This Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparation method is as follows:
1) take Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri by described proportioning, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri are washed respectively, after drying with microwave Chinese herbal medicine drying equipment, mixing, be ground into 60 order coarse powder, mix with the part by weight of deionized water by 1:5.5, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry;
2) in step 1) add in gained Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry 0.2% antifreeze, described antifreeze is ethanol, regulate its temperature to-14 DEG C, 20h is kept under this temperature conditions, then heat up with the intensification of 0.3 DEG C speed, be warming up to 0 DEG C, 25h is kept under this temperature conditions, then heat up with the intensification of 0.5 DEG C speed, be warming up to 50 DEG C, 10h is kept under this temperature conditions, send into Chinese-medicinal thermal-refluxing extraction group, negative pressure of vacuum is utilized to carry out extract at low temperature and cryoconcentration, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the process conditions of described extraction and concentration are: the operating temperature in extraction pot is 70 DEG C, extraction time is 1.5h, thickening temperature controls at 65 DEG C, be concentrated into crystallization to separate out completely, the operating pressure of extraction pot is 0.93MPa, maximumly maintain Chinese crude drug active ingredient not by drain evaporation.
This Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are for the preparation of anti-rectal cancer medicine.
Embodiment 4: a kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract is for raw material is prepared from Ampelopteris prolifera (Retz.) Copel. of 1:8 weight ratio and Radix rubi buergeri.
This Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparation method is as follows:
1) take Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri by described proportioning, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri are washed respectively, after drying with microwave Chinese herbal medicine drying equipment, mixing, be ground into 60 order coarse powder, mix with the part by weight of deionized water by 1:5.1, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry;
2) in step 1) add in gained Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry 0.02% antifreeze, described antifreeze is methanol, regulate its temperature to-20 DEG C, 10h is kept under this temperature conditions, then heat up with the intensification of 0.1 DEG C speed, be warming up to 0 DEG C, 15h is kept under this temperature conditions, then heat up with the intensification of 0.3 DEG C speed, be warming up to 46 DEG C, 5h is kept under this temperature conditions, send into Chinese-medicinal thermal-refluxing extraction group, negative pressure of vacuum is utilized to carry out extract at low temperature and cryoconcentration, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the process conditions of described extraction and concentration are: the operating temperature in extraction pot is 68 DEG C, extraction time is 1.2h, thickening temperature controls at 62 DEG C, be concentrated into crystallization to separate out completely, the operating pressure of extraction pot is 0.93MPa, maximumly maintain Chinese crude drug active ingredient not by drain evaporation.
Mix with the weight proportion of cyclophosphamide by 17:1 with Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the application in the anti-gastric cancer medicament of preparation.
Embodiment 5:
A kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are for raw material is prepared from Ampelopteris prolifera (Retz.) Copel. of 1:1 weight ratio and Radix rubi buergeri.
This Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparation method is as follows:
1) take Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri by described proportioning, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri are washed respectively, after drying with microwave Chinese herbal medicine drying equipment, mixing, be ground into 60 order coarse powder, mix with the part by weight of deionized water by 1:5.3, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry;
2) in step 1) add in gained Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry 0.011% antifreeze, described antifreeze is ethanol, regulate its temperature to-17 DEG C, 15h is kept under this temperature conditions, then heat up with the intensification of 0.2 DEG C speed, be warming up to 0 DEG C, 20h is kept under this temperature conditions, then heat up with the intensification of 0.4 DEG C speed, be warming up to 48 DEG C, 7.5h is kept under this temperature conditions, send into Chinese-medicinal thermal-refluxing extraction group, negative pressure of vacuum is utilized to carry out extract at low temperature and cryoconcentration, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the process conditions of described extraction and concentration are: the operating temperature in extraction pot is 69 DEG C, extraction time is 1.35h, thickening temperature controls at 63.5 DEG C, be concentrated into crystallization to separate out completely, the operating pressure of extraction pot is 0.93MPa, maximumly maintain Chinese crude drug active ingredient not by drain evaporation.
Mix with the weight proportion of cyclophosphamide by 22.5:1 with Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the application in the anti-esophageal cancer medicine of preparation.
Embodiment 6:
A kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are for raw material is prepared from Ampelopteris prolifera (Retz.) Copel. of 8:1 weight ratio and Radix rubi buergeri.
This Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparation method is as follows:
1) take Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri by described proportioning, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri are washed respectively, after drying with microwave Chinese herbal medicine drying equipment, mixing, be ground into 60 order coarse powder, mix with the part by weight of deionized water by 1:5.5, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry;
2) in step 1) add in gained Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry 0.2% antifreeze, described antifreeze is ethanol, regulate its temperature to-14 DEG C, 20h is kept under this temperature conditions, then heat up with the intensification of 0.3 DEG C speed, be warming up to 0 DEG C, 25h is kept under this temperature conditions, then heat up with the intensification of 0.5 DEG C speed, be warming up to 50 DEG C, 10h is kept under this temperature conditions, send into Chinese-medicinal thermal-refluxing extraction group, negative pressure of vacuum is utilized to carry out extract at low temperature and cryoconcentration, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the process conditions of described extraction and concentration are: the operating temperature in extraction pot is 70 DEG C, extraction time is 1.5h, thickening temperature controls at 65 DEG C, be concentrated into crystallization to separate out completely, the operating pressure of extraction pot is 0.93MPa, maximumly maintain Chinese crude drug active ingredient not by drain evaporation.
Mix with the weight proportion of cyclophosphamide by 28:1 with Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparing the application in drugs against colon cancer.
Embodiment 7: a kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract is for raw material is prepared from Ampelopteris prolifera (Retz.) Copel. of 1:8 weight ratio and Radix rubi buergeri.
This Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparation method is as follows:
1) take Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri by described proportioning, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri are washed respectively, after drying with microwave Chinese herbal medicine drying equipment, mixing, be ground into 60 order coarse powder, mix with the part by weight of deionized water by 1:5.1, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry;
2) in step 1) add in gained Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry 0.02% antifreeze, described antifreeze is methanol, regulate its temperature to-20 DEG C, 10h is kept under this temperature conditions, then heat up with the intensification of 0.1 DEG C speed, be warming up to 0 DEG C, 15h is kept under this temperature conditions, then heat up with the intensification of 0.3 DEG C speed, be warming up to 46 DEG C, 5h is kept under this temperature conditions, send into Chinese-medicinal thermal-refluxing extraction group, negative pressure of vacuum is utilized to carry out extract at low temperature and cryoconcentration, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the process conditions of described extraction and concentration are: the operating temperature in extraction pot is 68 DEG C, extraction time is 1.2h, thickening temperature controls at 62 DEG C, be concentrated into crystallization to separate out completely, the operating pressure of extraction pot is 0.93MPa, maximumly maintain Chinese crude drug active ingredient not by drain evaporation.
Mix with the weight proportion of pemetrexed by 13:1 with Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the application in the anti-gastric cancer medicament of preparation.
Embodiment 8:
A kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are for raw material is prepared from Ampelopteris prolifera (Retz.) Copel. of 1:1 weight ratio and Radix rubi buergeri.
This Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparation method is as follows:
1) take Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri by described proportioning, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri are washed respectively, after drying with microwave Chinese herbal medicine drying equipment, mixing, be ground into 60 order coarse powder, mix with the part by weight of deionized water by 1:5.3, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry;
2) in step 1) add in gained Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry 0.011% antifreeze, described antifreeze is methanol, regulate its temperature to-17 DEG C, 15h is kept under this temperature conditions, then heat up with the intensification of 0.2 DEG C speed, be warming up to 0 DEG C, 20h is kept under this temperature conditions, then heat up with the intensification of 0.4 DEG C speed, be warming up to 48 DEG C, 7.5h is kept under this temperature conditions, send into Chinese-medicinal thermal-refluxing extraction group, negative pressure of vacuum is utilized to carry out extract at low temperature and cryoconcentration, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the process conditions of described extraction and concentration are: the operating temperature in extraction pot is 69 DEG C, extraction time is 1.35h, thickening temperature controls at 63.5 DEG C, be concentrated into crystallization to separate out completely, the operating pressure of extraction pot is 0.93MPa, maximumly maintain Chinese crude drug active ingredient not by drain evaporation.
Mix with the weight proportion of pemetrexed by 17.5:1 with Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparing the application in drugs against colon cancer.
Embodiment 9:
A kind of Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract are for raw material is prepared from Ampelopteris prolifera (Retz.) Copel. of 8:1 weight ratio and Radix rubi buergeri.
This Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, preparation method is as follows:
1) take Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri by described proportioning, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri are washed respectively, after drying with microwave Chinese herbal medicine drying equipment, mixing, be ground into 60 order coarse powder, mix with the part by weight of deionized water by 1:5.5, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry;
2) in step 1) add in gained Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry 0.2% antifreeze, described antifreeze is ethanol, regulate its temperature to-14 DEG C, 20h is kept under this temperature conditions, then heat up with the intensification of 0.3 DEG C speed, be warming up to 0 DEG C, 25h is kept under this temperature conditions, then heat up with the intensification of 0.5 DEG C speed, be warming up to 50 DEG C, 10h is kept under this temperature conditions, send into Chinese-medicinal thermal-refluxing extraction group, negative pressure of vacuum is utilized to carry out extract at low temperature and cryoconcentration, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the process conditions of described extraction and concentration are: the operating temperature in extraction pot is 70 DEG C, extraction time is 1.5h, thickening temperature controls at 65 DEG C, be concentrated into crystallization to separate out completely, the operating pressure of extraction pot is 0.93MPa, maximumly maintain Chinese crude drug active ingredient not by drain evaporation.
Mix with the weight proportion of pemetrexed by 22:1 with Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the application in the anti-rectal cancer medicine of preparation.
Anti-tumor experiment:
1. test material
Experimental group 1 medicine: with the medicine of the embodiment of the present invention 1;
Experimental group 2 medicine: with the medicine of the embodiment of the present invention 2;
Experimental group 3 medicine: with the medicine of the embodiment of the present invention 6;
Positive controls medicine: Cyclophosphamide for injection, 0.20g/ props up, to give nude mice abdominal cavity injection after physiological saline solution during test;
Blank group medicine: nothing;
Laboratory animal: ALB/c-nu nude mouse, ♂, SPF level, 20 ~ 22g, totally 50, individual 10 of experimental group 1, experimental group 2, experimental group 3, positive controls, blank group;
Experimental tumor cell strain: stomach cancer cell Kato III, buys in Chinese Academy of Sciences's cell bank.
2. test method
Tumor cell line is cultivated: by stomach cancer cell Kato III cell recovery, carry out cellar culture, collecting cell suspension after adherent growth 2 generation, and be diluted to 10 with culture medium
7the concentration of individual/ml, subcutaneous injection is in 5 groups of nude mice bilateral oxters respectively, takes tumor, is cut into the fritter of 2 × 2mm size under gnotobasis, transplant in the one-sided axillary fossa of nude mice subcutaneous, start administration after according to said method going down to posterity after tumor grows up to.Treat that in body, the 3rd passage of tumor grows to 100mm
3rear beginning oral administration.
Experimental group 1 with the medicine of the embodiment of the present invention 1 with 0.5% sodium carboxymethyl cellulose (CMC) suspension will be made into after give nude mouse oral administration gavage by 200mg crude drug/kg dosage, once a day, continuous 28 days.
Experimental group 1 with the medicine of the embodiment of the present invention 2 with 0.5% sodium carboxymethyl cellulose (CMC) suspension will be made into after give nude mouse oral administration gavage by 200mg crude drug/kg dosage, once a day, continuous 28 days.
Experimental group 3 with the medicine of the embodiment of the present invention 6 with 0.5% sodium carboxymethyl cellulose (CMC) suspension will be made into after give nude mouse oral administration gavage by 200mg crude drug/kg dosage, once a day, continuous 28 days.
Positive drug control group, uses cyclophosphamide 20mg crude drug/kg, once a day, and continuous 28 days.
Blank group not administration process.
After execution, get tumor block, thymus and spleen, weigh and calculate tumour inhibiting rate, thymus coefficient and Spleen coefficient, formula is as follows: tumour inhibiting rate=(blank group tumor weight-administration group tumor heavy/blank group tumor weight) × 100%; Thymus index=chest gland weight/body weight (mg/g); Spleen to mean in number=spleen/body weight (mg/g).
3. result of the test
After being processed as stated above by mice, analyze tumor, experimental result is in table 1.
Table 1: each group medicine is to the inhibitory action of stomach cancer cell Kato III tumor
4. conclusion
Experimental result shows, compared with blank group, the embodiment of the present invention 1, embodiment 2, embodiment 6 medicine show good inhibitory action to stomach cancer cell Kato III tumor, tumour inhibiting rate reaches as high as 57.46%, and the embodiment of the present invention 1 medicine does not have inhibitory action to the increase of the body weight of mice while performance antitumor action.
Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in previous embodiment, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (10)
1. Ampelopteris prolifera (Retz.) Copel. and a Radix rubi buergeri composite extract, is characterized in that, is for raw material is prepared from Ampelopteris prolifera (Retz.) Copel. of 1:8 ~ 8:1 weight ratio and Radix rubi buergeri.
2. a kind of Ampelopteris prolifera (Retz.) Copel. according to claim 1 and Radix rubi buergeri composite extract, is characterized in that, described Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, the method preparation according to comprising the steps:
1) take Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri by described proportioning, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri are washed respectively, after drying with microwave Chinese herbal medicine drying equipment, mixing, be ground into 60 order coarse powder, mix with the part by weight of deionized water by 1:5.1 ~ 1:5.5, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry;
2) in step 1) add in gained Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri slurry 0.02 ~ 0.2% antifreeze, regulate its temperature to-20 ~-14 DEG C, 10 ~ 20h is kept under this temperature conditions, then heat up with the intensification of 0.1 ~ 0.3 DEG C speed, be warming up to 0 DEG C, 15 ~ 25h is kept under this temperature conditions, then heat up with the intensification of 0.3 ~ 0.5 DEG C speed, be warming up to 46 ~ 50 DEG C, 5 ~ 10h is kept under this temperature conditions, send into Chinese-medicinal thermal-refluxing extraction group, utilize negative pressure of vacuum to carry out extract at low temperature and cryoconcentration, obtain Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract.
3. preparation method according to claim 2, it is characterized in that, described step 2) the process conditions of extraction and concentration be: the operating temperature in extraction pot is 68 ~ 70 DEG C, extraction time is 1.2 ~ 1.5h, thickening temperature controls at 62 DEG C ~ 65 DEG C, be concentrated into crystallization to separate out completely, the operating pressure of extraction pot is 0.93MPa.
4. preparation method according to claim 2, is characterized in that, described antifreeze is methanol or ethanol.
5. a kind of Ampelopteris prolifera (Retz.) Copel. according to Claims 1-4 any one and Radix rubi buergeri composite extract are preparing the application in antitumor drug.
6. application according to claim 5, is characterized in that, described tumor is gastric cancer, esophageal carcinoma, colon cancer or rectal cancer.
7. application according to claim 5, is characterized in that, is to mix according to a certain weight ratio with cyclophosphamide with Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, is preparing the application in antitumor drug.
8. application according to claim 7, is characterized in that, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract and cyclophosphamide by weight proportion be 17:1 ~ 28:1.
9. application according to claim 8, is characterized in that, is to mix according to a certain weight ratio with pemetrexed with Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract, is preparing the application in antitumor drug.
10. application according to claim 9, is characterized in that, Ampelopteris prolifera (Retz.) Copel. and Radix rubi buergeri composite extract and pemetrexed by weight proportion be 13:1 ~ 22:1.
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