CN105012366B - A kind of bright moon grass polysaccharide and its application in preparing for immunological regulation and anti-tumor drug and functional food - Google Patents
A kind of bright moon grass polysaccharide and its application in preparing for immunological regulation and anti-tumor drug and functional food Download PDFInfo
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Abstract
A kind of application the present invention provides bright moon grass polysaccharide and its in preparing for immunological regulation and anti-tumor drug and functional food, bright moon grass polysaccharide produced by the present invention can promote mononuclear macrophage strain phagocytosis dimethyl diaminophenazine chloride and release nitric oxide；It can promote the proliferation of B cell and the proliferation of T cell；Immunosuppressed mice spleen and thymus index, raising can also be significantly improved in the proliferation of T cell and B cell, improve immunosuppressed mice leucocyte level；The growth of tumor tissues can obviously be inhibited；But also body can be protected, inhibit the decline of weight, and put on weight.And polysaccharide is the main composition of angelica keiskei koidz, has various health care functions, source belongs to pure natural, without side-effects, can be developed into immunological regulation, antitumor, anti-angiogenesis drug and health products, therefore apply future with good market.
Description
Technical field
The invention belongs to pharmaceutical technology fields, and in particular to a kind of bright moon grass polysaccharide and its preparing for immunological regulation and
Application in anti-tumor drug and functional food.
Background technology
In recent years, immunologic hypofunction, including primary and secondary immunodeficiency, shortage or impaired, by state
The extensive concern of inside and outside researcher, because themselves and the various complication induced, especially infect, often make the disease of patient
Feelings aggravate, it is difficult to treat, and can cause serious consequence or even threat to life.Therefore, safe improvement immunity of organism work(is found
The drug of energy will save more patients, have good application prospect in health-care industry.
In addition, tumour is the difficult project of 21st century medical field, it is the highest disease of the global death rate, finds high
The antitumor drug of effect low toxicity becomes the new trend of medicament research and development.The yeast beta-dextran found from the 1950s has anti-
The hope that function of tumor has given people new starts, and the antitumor activity of polysaccharide becomes new hot spot.At present from various fungies, plant
The polysaccharide of many antitumor activities has been isolated in object.The study found that polysaccharide is mainly antitumor by two main paths performances
Activity：One kind is the cytotoxic polysaccharide of tool, directly kills tumour cell, and one kind is the active polysaccharide of immunomodulator, main
Tumour cell is killed indirectly to reach by enhancing immunity of organisms.
Angelica keiskei koidz (Angelica keiskei Koidzumi) is also known as golden pheasant hair grass, and Angelica Keiskei also falls within composite family chrysanthemum Radix Notoginseng
Belong to, is perennial root herbaceous plant for many years, originates in Singapore.Angelica keiskei koidz is used with cauline leaf offerings, and abundant albumen is contained in cauline leaf
Matter, amino acid, flavones, vitamin, micro- isoreactivity ingredient, herb can be used as medicine, and pharmacological property is cold, micro- to have bitter taste.It should
Chinese herbal medicine main function be it is clearing heat and detoxicating, it is significant in efficacy in terms for the treatment of venomous snake bite.And drawn due to hot to every in human body
Various inflammation, such as gastritis, hepatitis, the bronchitis risen is effective in cure.It is known as " life-saving grass " or " god grass " civil.
Research has shown that polysaccharide is the important substance basis that Chinese herbal medicine plays unique curative effect, and polysaccharide can be immune thin by activating
Born of the same parents, promote cell factor to generate and express, and various immunoregulation effects are played to immune system.Bright moon grass polysaccharide is as it
In important composition ingredient, Recent study finds that both compounds all have anti-diabetic activity and blood pressure reduction effect, but
Immunological regulation for bright moon grass polysaccharide or bright moon grass polysaccharide and antitumor activity, have no correlative study.
Invention content
The object of the present invention is to provide a kind of bright moon grass polysaccharide and its preparing for immunological regulation and antitumor
Application in drug and functional food, the technical scheme is that clinic provides suitable immunocompromised patient, tumor patient
Or the drug or health products that sub-health population is taken.
For achieving the above object, the present invention is achieved by the following scheme：
A kind of bright moon grass polysaccharide, it is made by following preparation method：By the cauline leaf drying and crushing of angelica keiskei koidz and it is sieved
To angelica keiskei koidz powder, the water for accounting for 10-30 times of quality of angelica keiskei koidz powder, 80-100 DEG C of refluxing extraction 2-4h are added, centrifugation takes
Clear liquid；It is added into the precipitation for remove supernatant and accounts for the water for precipitating 5-15 times of quality, 80-100 DEG C of refluxing extraction 2-4h, from
The heart takes supernatant；The supernatant for merging the acquisition that flows back twice, into supernatant, addition ethyl alcohol makes ethyl alcohol final concentration of 75%, alcohol precipitation
Overnight, supernatant is removed in centrifugation, and for precipitation after removing protein and decoloration, freeze-drying finally obtains the bright moon grass polysaccharide.
The angelica keiskei koidz polysaccharide molecular weight is 20-2000KDa, total sugar content 20-85.0%, glucuronic acid content 30-
70%.
The bright moon grass polysaccharide contains arabinose, rhamnose, mannose, galactolipin, xylose, glucose, galacturonic
It is one or more in acid, glucuronic acid or Glucosamine.
The present invention also provides the bright moon grass polysaccharides in preparing for immunoregulatory drug and functional food
Using.
Above-mentioned technical proposal is further improved：There is the bright moon grass polysaccharide enhancing mononuclear macrophage strain cell to gulp down
Bite the ability of dimethyl diaminophenazine chloride.
Above-mentioned technical proposal is further improved：The bright moon grass polysaccharide has enhancing mononuclear macrophage strain cell point
Secrete nitric oxide production ability.
Above-mentioned technical proposal is further improved：The T lymphopoiesis that the bright moon grass polysaccharide can improve mouse refers to
Number and B lymphocyte proliferation index.
Above-mentioned technical proposal is further improved：The bright moon grass polysaccharide can improve the index and spleen index of mouse and thymus gland refers to
Number.
The present invention also provides the bright moon grass polysaccharide answering in preparing for anti-tumor drug and functional food
With.
Above-mentioned technical proposal is further improved：The bright moon grass polysaccharide can obviously inhibit the growth of tumor tissues and bright
The aobvious migration for inhibiting venous endothelial cell.
Technical scheme of the present invention has the following advantages that：The present invention provides the preparation method of specific bright moon grass polysaccharide, systems
The bright moon grass polysaccharide obtained can promote RAW264.7 mononuclear macrophage strains phagocytosis dimethyl diaminophenazine chloride and release nitric oxide；It can promote
The proliferation of the B cell of LPS inductions, and promote the proliferation of the T cell of ConA inductions；Can also significantly improve immunosuppressed mice spleen and
Thymus index, raising improve immunosuppressed mice leucocyte level in the proliferation of T cell and B cell；It can obviously inhibit swollen
The growth of tumor tissue；But also body can be protected, inhibit the decline of weight, and put on weight；Venous endothelial can obviously be inhibited
The migration of cell；Bright moon grass polysaccharide has stronger blood vessel formation against function.
Bright moon grass polysaccharide of the present invention has specific immunity and nospecific immunity, humoral immunity and cellular immunity
There is facilitation, while having and inhibiting tumour growth and vascular migration effect, and polysaccharide is the main composition of angelica keiskei koidz,
With various health care functions, source belongs to pure natural, without side-effects, can be developed into immunological regulation, antitumor, anti-angiogenic drugs
Product and health products, therefore apply future with good market.
Description of the drawings
Fig. 1 be in the present invention bright moon grass polysaccharide to the inhibiting rate experimental result of tumour growth.
Specific implementation mode
Technical scheme of the present invention is further described in detail in the following with reference to the drawings and specific embodiments.
The extraction of embodiment 1, bright moon grass polysaccharide prepares and analysis
The preparation method of bright moon grass polysaccharide is as follows：50g angelica keiskei koidz stem and leaf powders are taken, the deionized water of 20 times of quality is added,
80-100 DEG C of refluxing extraction 2h, centrifugation, takes supernatant, and precipitates the deionized water that 5 times of quality are added, 80-100 DEG C of refluxing extraction
2h, centrifugation, merging filtrate, a certain amount of 95% ethyl alcohol, which is added, in filtrate makes ethyl alcohol final concentration of 75%, and alcohol precipitation is overnight, and centrifugation is gone
Supernatant, precipitation is after removing protein and decoloration, and freeze-drying, the bioactive polysaccharide finally obtained is that angelica keiskei koidz of the present invention is more
Sugar.
The bright moon grass polysaccharide is through HPLC molecular weight determinations, weight average molecular weight 20-2000KDa, total sugar content 20-
85.0%, glucuronic acid content 30-70%, the polysaccharide for forming its structure have arabinose, rhamnose, mannose, galactolipin, wood
One or more of sugar, glucose, galacturonic acid or Glucosamine.
Embodiment 2, bright moon grass polysaccharide swallow RAW264.7 cells the facilitation of dimethyl diaminophenazine chloride
Mouse monokaryon macrophage RAW264.7 cells, with the DMEM high glucose medium cultures containing 10% fetal calf serum.It takes
Exponential phase cell is with 1.0 × 105The concentration of cells/mL is inoculated in 96 orifice plates, in 5%CO2, 37 DEG C, saturated humidity
In constant incubator culture to cell it is adherent after (about 6-8h), be added bright moon containing various concentration grass polysaccharide (1,5,10,25,50,
100 μ g/ml) totally 6 concentration, positive drug are lentinan, 2 μ g/ml of final concentration, 4 parallel holes of each concentration setting, culture
It is taken out after 12h, siphons away original culture medium, room temperature PBS is washed one time, and the dimethyl diaminophenazine chloride dye liquor of 100 μ L 0.075% is added per hole, is continued
It is conventional to be incubated 30min, dimethyl diaminophenazine chloride is siphoned away, PBS is washed twice, and room temperature lysate cracks (lysate：Absolute ethyl alcohol:Glacial acetic acid=1:
1) 20min, with colorimetric at microplate reader 540nm, surveys its respective OD value until after being completely dissolved, and calculates the phagocytosis of RAW264.7 cells
The ability of dimethyl diaminophenazine chloride.
Phagocytic index=dosing holes OD values/blank well OD values
1 bright moon grass polysaccharide of table swallows RAW264.7 cells the influence of dimethyl diaminophenazine chloride
| Grouping | OD values |
| Blank group | 0.88±0.09 |
| Bright moon grass polysaccharide (1 μ g/ml) | 0.93±0.13 |
| Bright moon grass polysaccharide (5 μ g/ml) | 0.98±0.07 |
| Bright moon grass polysaccharide (10 μ g/ml) | 1.03±0.05 |
| Bright moon grass polysaccharide (25 μ g/ml) | 1.06±0.03 |
| Bright moon grass polysaccharide (50 μ g/ml) | 1.14±0.14* |
| Bright moon grass polysaccharide (100 μ g/ml) | 1.07±0.08 |
Experiment conclusion：As can be seen from Table 1, bright moon grass polysaccharide all has centainly within the scope of 1 μ of μ g/ml~100 g/ml
Enhance the ability of RAW264.7 cells phagocytosis dimethyl diaminophenazine chloride, and certain dosage accordance with tolerance is presented, when dosage is 50 μ g/ml
Best results have significant difference compared with blank group.Illustrate that bright moon grass polysaccharide can significantly improve mononuclear macrophage strain
Phagocytic activity, to improve the nospecific immunity of body.
Embodiment 3, bright moon grass polysaccharide are to promoting RAW264.7 cells to secrete nitric oxide production effect
Mouse monokaryon macrophage RAW264.7 cells, with the DMEM high glucose medium cultures containing 10% fetal calf serum.It takes
Exponential phase cell is inoculated in the concentration of 1.0x105cells/mL in 96 orifice plates, in 5%CO2,37 DEG C, saturated humidity
In constant incubator culture to cell it is adherent after (about 6-8h), be added bright moon containing various concentration grass polysaccharide (1,5,10,25,50,
100 μ g/ml) totally 6 concentration, positive drug is lentinan, 2 μ g/ml of final concentration, and 4 parallel holes, culture is arranged in each concentration
It takes out afterwards for 24 hours, takes 50 μ L cultures based in another 96 orifice plate, (the green skies are biological according to nitric oxide detection kit specification
Reagent Company), i.e., Grignard Reagent method detects.Standard curve is drawn by standard items of NaNO2 first, finally with NaNO2 content marks
Determine the content of nitric oxide of cell release.
2 bright moon grass polysaccharide of table discharges nitric oxide production influence to RAW264.7 cells
| Grouping | Content of nitric oxide (μM) |
| Blank group | 2.88±0.21 |
| Bright moon grass polysaccharide (5 μ g/ml) | 5.12±0.11*** |
| Bright moon grass polysaccharide (10 μ g/ml) | 4.37±0.27*** |
| Bright moon grass polysaccharide (25 μ g/ml) | 4.24±0.16*** |
| Bright moon grass polysaccharide (50 μ g/ml) | 3.62±0.22*** |
| Bright moon grass polysaccharide (100 μ g/ml) | 3.42±0.18*** |
Experiment conclusion：As can be seen from Table 2, bright moon grass polysaccharide all has centainly within the scope of 5 μ of μ g/ml~100 g/ml
Enhance RAW264.7 cells and secrete nitric oxide production ability, and certain dosage accordance with tolerance is presented, is 5 μ g/ml in dosage
When best results, compared with blank group have pole significant difference.It is thin to illustrate that bright moon grass polysaccharide can significantly improve monokaryon macrophage
The Secretion of Nitric Oxide ability of born of the same parents' strain, to improve the nospecific immunity of body.The nitric oxide molecule that immune system generates,
The microorganism of intrusion human body can not only be resisted, but also the breeding of cancer cell can be prevented to a certain extent, prevents tumour thin
Born of the same parents are spread.Illustrate that bright moon grass polysaccharide may be used as immunomodulator or antitumor drug and adjuvant.
Embodiment 4, bright moon grass polysaccharide act on the mouse spleen cell proliferation of in vitro culture
After Kunming mouse cervical dislocation is put to death, 75% alcohol disinfecting 3-5min, super-clean bench dissects mouse, remove mice spleen
Dirty and thymus gland, PBS are cleaned, and grind extracting spleen cell and thymocyte, are counted, splenocyte is with 4 × 107The density of cells/mL, chest
Gland cell is with 1.5 × 108The density of cells/mL is inoculated in 96 orifice plates, be added after 4h various concentration bright moon grass polysaccharide (10,
25,50,100,200 μ g/ml) positive drug is lentinan, 2 μ g/ml of final concentration, 4 parallel holes are arranged in each concentration, 5%
CO2, 37 DEG C, 72h is trained in the constant incubator of saturated humidity, experiment terminates preceding 4h, and the MTT solution of 20 μ L5% is added per hole, after
After continuous conventional incubation 4h, 100 μ L, tri- liquid is added, 37 ° stand overnight, and dissolve the first a ceremonial jade-ladle, used in libation of generation, it is surveyed at microplate reader 490nm
The OD values in each hole, calculate Proliferating antigen Ki67, and experimental result is repeated 4 times.
Proliferation index=dosing holes OD values/blank well OD values
3 angelica keiskei koidz Polysaccharides on Mice Splenic vein hemodynamics of table and value-added influence
| Grouping | T cell proliferation index | B cell value added index |
| Lentinan group | 1.56±0.07 | 1.25±0.039 |
| Bright moon grass polysaccharide (10 μ g/ml) | 1.43±0.25 | 1.17±0.12 |
| Bright moon grass polysaccharide (25 μ g/ml) | 1.51±0.22* | 1.27±0.13* |
| Bright moon grass polysaccharide (50 μ g/ml) | 1.52±0.21* | 1.28±0.21** |
| Bright moon grass polysaccharide (100 μ g/ml) | 1.42±0.22 | 1.24±0.17 |
| Bright moon grass polysaccharide (200 μ g/ml) | 1.40±0.17 | 1.21±0.11 |
Experiment conclusion：As can be seen from Table 3, bright moon grass polysaccharide all has enhancing within the scope of 10 μ of μ g/ml~200 g/ml
The proliferation of mouse T lymphocyte and bone-marrow-derived lymphocyte, and certain dosage accordance with tolerance is presented, it is 50 μ g/ml timeliness in dosage
Fruit is best, has significant difference compared with blank group, shows that bright moon grass polysaccharide can improve specific immune response.
The immunological enhancement for the immunosuppressed mice that embodiment 5, angelica keiskei koidz Polysaccharides On Cyclophosphamide induce
Experimental method：Healthy male mouse of kunming 72, weight 18-22g is chosen, is randomly divided into 7 groups every group 12, respectively
For blank control group, cyclophosphamide model group, the high, normal, basic dosage group of bright moon grass polysaccharide, lentinan control group.Successive administration 15
It, respectively in the 9th, 10,11 3 day continuous injection cyclophosphamide 60mg/kg of administration, and dissects mouse the 15th, plucks eyeball and take
Blood measures peripheral hemogram, and wins spleen, thymus gland measurement organ index.
4 experiment packet of table is administered
The immunosuppressed mice weight of 5 angelica keiskei koidz Polysaccharides On Cyclophosphamide of table induction and the influence of organ index
Experiment conclusion：As can be seen from Table 5, model group mouse spleen index, thymus index have pole compared with blank group
Significant difference shows model success, and bright moon grass polysaccharide administration group can improve the index and spleen index and thymus index of mouse, and
Certain dosage accordance with tolerance, wherein middle dose group best results is presented, there is significant difference compared with model group.As a result table
Bright, bright moon grass polysaccharide can improve the immunity of mouse, and it is the potentiality of immunizing health product or drug to have exploitation.
6 mouse of table is tested in body lymphocyte transformation
| Grouping | T cell is proliferated conversion ratio/OD | B cell increment conversion rate/OD |
| Blank group | 0.61±0.03### | 0.52±0.07### |
| Model group | 0.51±0.06 | 0.39±0.05 |
| Positive drug group | 0.52±0.06* | 0.49±0.04*** |
| Angelica keiskei koidz low dose group | 0.55±0.04** | 0.57±0.03*** |
| Angelica keiskei koidz middle dose group | 0.65±0.05*** | 0.61±0.05*** |
| Angelica keiskei koidz high dose group | 0.57±0.07 | 0.51±0.09 |
Experiment conclusion：As can be seen from Table 6, model group mouse T lymphocyte proliferation index and B lymphocyte proliferation index
There is pole significant difference compared with blank group, show model success, and bright moon grass polysaccharide administration group can significantly improve mouse
T lymphopoiesis index and B lymphocyte proliferation index, and certain dosage accordance with tolerance is presented, wherein middle dose group is imitated
Fruit is best, has significant difference compared with model group.The result shows that bright moon grass polysaccharide can improve the immunity of mouse, tool
It is the potentiality of immunizing health product or drug to have exploitation.
The influence of the immunosuppressed mice blood picture of 7 angelica keiskei koidz Polysaccharides On Cyclophosphamide of table induction
Experiment conclusion：As can be seen from Table 7, model group murine interleukin number has pole conspicuousness poor compared with blank group
It is different, show model success, and bright moon grass polysaccharide administration group can significantly improve the leukocyte count of mouse, and certain dosage is presented
Accordance with tolerance, wherein middle dose group best results have significant difference compared with model group.The result shows that bright moon grass polysaccharide can
To improve the immunity of mouse, it is the potentiality of immunizing health product or drug to have exploitation.
Embodiment 6, bright moon grass polysaccharide are to the inhibiting effect of tumor-bearing mice tumour growth and neoplasm lung metastasis
LLC cells are cultivated under appropriate conditions, when cell growth to logarithmic phase, cell is resuspended, counts, inoculation,
It is passed in body.Later 1 cubic millimeter of tumor mass is inoculated in mouse right fore armpit.After 1 week, wait for that tumor grows to 9 cubic millimeters
Mouse, is grouped, i.e. angelica keiskei koidz senior middle school low dose group, cyclophosphamide positive controls, every group 12 by left and right at random by weight.It is empty
White group gives physiological saline 20mLkg-1d-1, administration group give bright moon grass polysaccharide dosage be 25,50,100mgkg-1
D-1 and positive controls give cyclophosphamide 30mgkg-1d, and gastric infusion designs stock concentrations, often according to dosage
Intragastric administration on mice 0.4mL or so, successive administration 21 days.Knurl weight is carried out to measure and lung's metastatic nodules, observation mouse weight change
Change, and calculates tumor control rate and lung's metastasis suppressor rate.
Inhibition efficiency of the 8 bright moon grass polysaccharide of table to tumour
The inhibition efficiency that 9 bright moon grass polysaccharide of table shifts tumour lung
As seen from Figure 1, bright moon grass polysaccharide can effectively inhibit the growth of tumour, and tumour inhibiting rate 46% shows bright moon
The tumor killing effect of grass polysaccharide is good.And there is the trend of weight loss during medication in cyclophosphamide, but angelica keiskei koidz group
Weight do not decline, ascendant trend is presented instead, shows that angelica keiskei koidz polysaccharide medicine toxicity is low, to animal also have protection
The effect of damage.
Meanwhile bright moon grass polysaccharide can significantly inhibit the spontaneous lung transfer of Lewis lung cancer, inhibit efficiency to be up to 47.2, table
Obviously the moon, grass polysaccharide had effective inhibiting effect to the transfer invasion of tumour.
To sum up, the present invention extracts polysaccharide active components by raw material of angelica keiskei koidz, and has carried out corresponding system experimentation, root
It according to the experimental result of embodiment 2 and 3, can prove on a cellular level, bright moon grass polysaccharide can promote RAW264.7 monokaryon macrophages
Cell strain swallows dimethyl diaminophenazine chloride and release nitric oxide, shows that bright moon grass polysaccharide has certain adjusting immunocompetence, can be developed into
Drug or food are treated in immune smelting.
According to the lymphocytes in vitro conversion test of embodiment 4 as a result, bright moon grass polysaccharide can promote the B of LPS inductions thin
The proliferation of born of the same parents, and promote the proliferation of the T cell of ConA inductions, so bright moon grass polysaccharide can enhance the cellular immunity of body, it can
Food and drug for improving body immunity.
According to the experimental result of embodiment 5, bright moon grass polysaccharide can significantly improve immunosuppressed mice spleen and thymus index, carry
Height improves immunosuppressed mice leucocyte level in the proliferation of T cell and B cell；Therefore, bright moon grass polysaccharide has relatively strong
Immunoregulatory activity, can be provided in clinical level and a kind of be very suitable for the drug or function that immunologic hypofunction crowd takes
Property food.
According to the experimental result of embodiment 6, bright moon grass polysaccharide can obviously inhibit the growth of tumor tissues；But also it can protect
Body is protected, inhibits the decline of weight, and put on weight；Therefore, bright moon grass polysaccharide has stronger antitumor action.
The bright moon grass polysaccharide with pharmaceutically acceptable drug or food, health care product auxiliary material are mixed to form various forms
Powder, paste, pulvis, tablet, injection, aqua or injection.Can be used for clinical provide is suitble to immunocompromised patient, tumour to suffer from
Drug or health products that person or sub-health population are taken and as one of combination, compounding composition with making adjustments immune, antitumor use
On the way.Oral, subcutaneous, intravenous injection or anal intestine administration can be taken when in use；The use of injection can arbitrarily select physiology salt
Water, glucose, stabilizer, suspending agent or emulsifier etc..
To sum up, the present invention extracts polysaccharide active components by raw material of angelica keiskei koidz, and it is good that inside and outside experiment shows that it has
Immune-enhancing effect and it is antitumor, inhibit metastases effect, can be applied to immunological regulation and anticancer usage.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than is limited；Although with reference to aforementioned reality
Invention is explained in detail for example, to those skilled in the art, still can be to previous embodiment institute
The technical solution of record is modified or equivalent replacement of some of the technical features；And these modifications or substitutions, and
The essence of corresponding technical solution is not set to be detached from the spirit and scope of claimed technical solution of the invention.
Claims (6)
1. a kind of bright moon grass polysaccharide, it is characterised in that it is made by following preparation method：By the cauline leaf drying and crushing of angelica keiskei koidz
And be sieved and obtain angelica keiskei koidz powder, add the water for accounting for 10-30 times of quality of angelica keiskei koidz powder, 80-100 DEG C of refluxing extraction 2-4h,
Centrifugation, takes supernatant；The water for accounting for 5-15 times of quality of the precipitation is added into the precipitation for remove supernatant, 80-100 DEG C of reflux carries
Take 2-4h, centrifuging and taking supernatant；The supernatant for merging the acquisition that flows back twice, into supernatant, addition ethyl alcohol keeps ethyl alcohol final concentration of
75%, alcohol precipitation is overnight, and supernatant is removed in centrifugation, and for precipitation after removing protein and decoloration, freeze-drying finally obtains the bright moon grass polysaccharide；It is described
Angelica keiskei koidz polysaccharide molecular weight is 20-2000KDa, total sugar content 20-85.0%, glucuronic acid content 30-70%；The angelica keiskei koidz
Polysaccharide contains arabinose, rhamnose, mannose, galactolipin, xylose, glucose, galacturonic acid, glucuronic acid or amino
It is one or more in glucose.
2. application of the bright moon grass polysaccharide described in claim 1 in preparing for immunoregulatory drug and functional food, institute
The T lymphopoiesis index and B lymphocyte proliferation index of mouse can be improved by stating moon grass polysaccharide clearly.
3. bright moon grass polysaccharide according to claim 2 answering in preparing for immunoregulatory drug and functional food
With, it is characterised in that：The bright moon grass polysaccharide has the ability of enhancing mononuclear macrophage strain cell phagocytosis dimethyl diaminophenazine chloride.
4. bright moon grass polysaccharide according to claim 2 answering in preparing for immunoregulatory drug and functional food
With, it is characterised in that：There is the bright moon grass polysaccharide enhancing mononuclear macrophage strain cell to secrete nitric oxide production ability.
5. bright moon grass polysaccharide according to claim 2 answering in preparing for immunoregulatory drug and functional food
With, it is characterised in that：The bright moon grass polysaccharide can improve the index and spleen index and thymus index of mouse.
6. application of the bright moon grass polysaccharide described in claim 1 in preparing for anti-tumor drug and functional food, described
Bright moon grass polysaccharide can obviously inhibit the growth of tumor tissues and the apparent migration for inhibiting venous endothelial cell.
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| JP2006280338A (en) * | 2005-04-05 | 2006-10-19 | Ashitaba Percent:Kk | Method for producing ashitaba tea |
| CN102669352A (en) * | 2012-05-31 | 2012-09-19 | 贵州大学 | Hypolipidemic and hypoglycemic bitter buckwheat tea and preparation method thereof |
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