CN105213059A - A kind of mid distal femur Cranial defect model and construction method thereof and application - Google Patents
A kind of mid distal femur Cranial defect model and construction method thereof and application Download PDFInfo
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- CN105213059A CN105213059A CN201510627050.7A CN201510627050A CN105213059A CN 105213059 A CN105213059 A CN 105213059A CN 201510627050 A CN201510627050 A CN 201510627050A CN 105213059 A CN105213059 A CN 105213059A
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Abstract
The invention provides a kind of construction method of mid distal femur Cranial defect model.The construction method of described Cranial defect model, comprising: after (1) anesthetized animal, gets animal hind leg preserved skin, sterilization, and before lower in the femur of animal, otch is made in outside, appears mid distal femur; (2) at the femur of mid distal femur excision different size, manufacture Cranial defect, irrigate, sew up wrapping, obtain described mid distal femur Cranial defect model.Mid distal femur Cranial defect operative approach in the present invention, avoid important blood vessel, nerve, the damage of secondary in Cranial defect model process of establishing and hemorrhage can be reduced to greatest extent, the uniformity of model, reproducible, be conducive to the repairing effect of simulating clinical Cranial defect.Present invention also offers based on the obtained mid distal femur Cranial defect model of the method and application thereof.
Description
Technical field
The present invention relates to medical care evaluation, medical detection field, be specifically related to a kind of mid distal femur Cranial defect model and construction method thereof and application.
Background technology
In Tissue Engineering Study field, standardized animal model is part indispensable in experimentation, is to evaluate biomaterial performance, construction strategy quality and observe seed cell to lapse to the necessary carrier of mechanism.The treatment of Cranial defect is the difficult point of orthopaedics, plastic surgery, decorative sursery etc. always, is also research emphasis for a long time and focus, set up rational Cranial defect animal model be research in an important step.
Common Cranial defect model mainly contains the Cranial defect such as radius, tibia model, but there are no Mus femur Cranial defect model.Radius, fibula, skull Cranial defect are confined to the size of bone own, cannot complete the research of large scale Cranial defect and the research of relevant large scale bone biologic material and implant.And radius and tibia have ulna and fibula companion row respectively in dissection, although excised radius or the medial tibial periosteal of corresponding length in the process of analogue formation, but in knitting process, can not get rid of the ulna closely adjacent with it or fibula periosteum to Cranial defect district skeletonization, and the bone formation effect of this film skeletonization inevitably affects the reliability of experimental result.
For completing the research of Cranial defect aspect more objectively, be necessary to build a kind of uniformity, the reproducible and Cranial defect model that interference factor is few.
Summary of the invention
In view of this, the invention provides a kind of mid distal femur Cranial defect model and construction method thereof and application.The present invention adopts femur to prepare Cranial defect model, the bone formation effect of film skeletonization can be avoided, mid distal femur Cranial defect operative approach in the present invention, avoid important blood vessel, nerve, the damage of secondary in Cranial defect model process of establishing and region of anatomy relative constancy that is hemorrhage, neural, blood vessel can be reduced to greatest extent, make a variation little, the uniformity of model, reproducible, be conducive to this Cranial defect model for object of study is to study the repairing effect of clinical Cranial defect.
First aspect, the invention provides a kind of construction method of mid distal femur Cranial defect model, comprises the steps:
(1) after anesthetized animal, get animal hind leg preserved skin, sterilization, before lower in the femur of animal, otch is made in outside, appears mid distal femur;
(2) at the femur of mid distal femur excision different size, manufacture Cranial defect, irrigate, sew up wrapping, obtain described mid distal femur Cranial defect model.
As described in the present invention, femur is the main heavy burden bone of mammal lower limb, and dissection is single bone traveling, is again the position that exogenous injury of human body fracture and Cranial defect incidence rate are higher, and therefore selecting femur to set up Cranial defect model has better clinical meaning.And the anatomical position relative constancy of nerve around femur and blood vessel, make a variation little.
Preferably, in described step (1), the animal of employing is rat, mice or rabbit, but is not limited thereto.
More preferably, in described step (1), the animal of employing is SD (SpragueDawley) rat.
In the embodiment of the present invention, preferred SD rat builds model, and the cost of Mus is low, be easy to raise and management; In addition, Mus femur is femoral shaft as alone weight-bearing bone, has more structural similarity and function homology with people's long bone, post operative recovery procedures and human body internal and external environment similar, be used for making Cranial defect model more accurately and objective.
Preferably, the way appearing mid distal femur described in is: before lower in the femur of animal, otch is made in outside, and cut skin, subcutaneous tissue and deep fascia, blunt separation, appears mid distal femur.
Further preferably, described blunt separation is specially: enter from the musculus lateralis externi gap blunt separation between vastus lateralis and biceps femoris.
As described in the present invention, when selecting different types of animal as modeling animal, according to different mode animal, or different animal strains, remove the femur of different size, manufacture the bone injury of different size.
In a preferred embodiment, in step (2), the length of the femur excised is 10-15mm.
In another preferred embodiment of the present invention, the diameter of the femur excised is 1.5-2mm.
Preferably, the described femur at mid distal femur excision different size, is specially: empirically design adopts orthopaedics scroll saw or bone drill to excise periosteum and the backbone of corresponding size at mid distal femur respectively, manufactures Cranial defect.
Preferably, in step (2), after described manufacture Cranial defect, further comprising the steps of: to put into orthopedics biological material implant at Cranial defect position.
Described orthopedics biological material implant comprises silicate bio-vitric, magnesium alloy or magnesium copper alloy, and silicate bio-vitric can be borosilicate bio-vitric or aluminum fluoride glass, but is not limited thereto.
Preferably, in step (2), after described dressing wrapping, also comprise: postoperative for three days on end to otch sterilization, intramuscular injection penicillin sodium 200,000 U, with prevention infection.
The present invention builds mid distal femur Cranial defect model, the preparation method of described model is simple, the uniformity, reproducible of the Cranial defect model built, after model is set up, the misery of the surviving of laboratory animal, the postoperative laboratory animal of recovery rate is little, activity is substantially unaffected, does not have obvious tissue reaction, implementation model set up quick, effective, save time, laborsaving.The present invention is that the associated treatment research of Cranial defect provides necessary carrier, and to providing certain reference frame for what prevent Cranial defect clinically on physiology, pathology, can be widely used in the correlational studyes such as bone tissue engineer, bone wound, skeletonization.
Second aspect, the invention provides a kind of mid distal femur Cranial defect model, described mid distal femur Cranial defect model builds by the following method: after (1) anesthetized animal, gets animal hind leg preserved skin, sterilization, before lower in the femur of animal, otch is made in outside, appears mid distal femur; (2) at the femur of mid distal femur excision different size, manufacture Cranial defect, irrigate, sew up wrapping, obtain described mid distal femur Cranial defect model.
In a preferred embodiment, described mid distal femur Cranial defect model adopts constructed by construction method as described in relation to the first aspect.
The third aspect, the construction method that the invention provides mid distal femur Cranial defect model is as described in relation to the first aspect preparing the application in femur Cranial defect model.
Preferably, when described application, put into the medicine of orthopedics biological material implant or treatment bone injury at Cranial defect position.
Accompanying drawing explanation
The schematic diagram of the construction method of the mid distal femur Cranial defect model that Fig. 1 provides for the embodiment of the present invention 1;
The flow chart of the construction method of the mid distal femur Cranial defect model that Fig. 2 provides for the embodiment of the present invention 2, wherein in A, arrow is operative incision, and B is for manufacture Cranial defect with bone drill, and C is for put into orthopedics biological material at Cranial defect place;
The Micro-CT scanning X-Y scheme of mid distal femur Cranial defect model after implanting orthopedics biological material that Fig. 3 provides for the embodiment of the present invention 2.
Detailed description of the invention
The following stated is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications are also considered as protection scope of the present invention.
Without outside special instruction in the embodiment of the present invention, agents useful for same and consumptive material are commercial goods.
Embodiment 1
The flow chart of the construction method of the mid distal femur Cranial defect model that Fig. 1 provides for the embodiment of the present invention, composition graphs 1, present embodiments provides a kind of construction method of mid distal femur Cranial defect model, comprises the steps:
(1) mid distal femur is appeared:
Get SD (SpragueDawley) rat at 6 monthly ages as laboratory animal, male and female, adaptability is fed 1 week.Experimental mouse is carried out lumbar injection chloral hydrate to anaesthetize, dosage is 300mg/kg.Anaesthetize successfully, take ventricumbent position that animal is fixed on animal experiment operating table, adopt cream of shedding to take off except hind leg Mus hair, the sterilization of art district, drape.Get lower front outside in Mus femur and make otch, but its ligament can not be cut off, cut skin, subcutaneous tissue and deep fascia, enter along from the musculus lateralis externi gap blunt separation between vastus lateralis and biceps femoris, appear mid distal femur (comprising lateral condyle), try not to peel off the muscle group that lateral condyle adheres to;
(2) empirically design adopts orthopaedics scroll saw or bone drill to excise periosteum and the backbone of corresponding size at mid distal femur respectively, takes the femur of different size away, manufactures Cranial defect.Art finishes with after normal saline flushing wound, sews up sarolemma and subcutaneous tissue, otch, sterilization otch, and after sewing up, dressing wrapping, obtains mid distal femur Cranial defect model; Postoperatively do not do any fixing, for three days on end to otch sterilization, intramuscular injection penicillin sodium 200,000 U, with prevention infection.
In the present embodiment, in step (2), the length of the femur excised is 15mm, and the diameter of the femur excised is 2mm.
Postoperative Bone defective region gross findings: postoperative 7 days, the wound of Mus all healed, the granulation tissue that postoperative 5-6 Zhou Shijian Cranial defect district has matter softer, without obvious callus formation.There is a small amount of thinner callus in 12 weeks after operation Shi Jian Cranial defect district, and near two-section epiphysis, Cranial defect district is soft tissue parcel.Postoperative 18 Zhou Shijian Cranial defect district callus formation comparatively before slightly increase, but Cranial defect district still without bone connect, in it, major part remains by the filling of granulation cicatrix, and Cranial defect does not heal.Lacking property of the section Cranial defect that this experimental result also demonstrates the long 15mm of femur is that self can not heal, and the Cranial defect model that therefore this experiment is set up is successful.
Embodiment 2
A construction method for mid distal femur Cranial defect model, comprises the steps:
(1) appear mid distal femur, operating procedure is with embodiment 1;
(2) orthopaedics miniature is adopted to be drilled in periosteum and the backbone that mid distal femur excises corresponding size, take the femur of different-diameter away, manufacture Cranial defect, put into the orthopedics biological material of corresponding size, wherein, the diameter of the femur excised is 2mm, and orthopedics biological material is specially silicate bio-vitric clavate support;
Art finishes with after normal saline flushing wound, sews up sarolemma and subcutaneous tissue, otch, sterilization otch, and after sewing up, dressing wrapping, obtains mid distal femur Cranial defect model; Postoperatively do not do any fixing, for three days on end to otch sterilization, intramuscular injection penicillin sodium 200,000 U, with prevention infection.
For absolutely proving beneficial effect of the present invention, the embodiment of the present invention 2 provides the micro-Micro-CT X-Y scheme of hypomere Cranial defect after implanting orthopedics biological material in rat femur, as shown in Figure 3.In figure 3, A figure is the Micro-CT X-Y scheme of operation rat after 4 weeks, and B figure is the Micro-CT X-Y scheme of operation rat after 6 weeks.As can be seen from Figure 3, after implanting 4 weeks, silicate bio-vitric clavate stent graft is slightly degraded, and Peripheral Bone defect area has skeletonization, but with Cranial defect gap still obviously (A schemes); After performing the operation 6 weeks, silicate bio-vitric clavate stent graft periphery has obvious degradation, and Peripheral Bone defect area skeletonization is obvious simultaneously, and the gap between implant periphery and bone is visible (B schemes) hardly.
Above-mentioned the results show, mid distal femur Cranial defect model provided by the invention is the ideal model of the therapeutic effect of research orthopedics biological material.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. a construction method for mid distal femur Cranial defect model, is characterized in that, comprises the steps:
(1) after anesthetized animal, get animal hind leg preserved skin, sterilization, before lower in the femur of animal, otch is made in outside, appears mid distal femur;
(2) at the femur of mid distal femur excision different size, manufacture Cranial defect, irrigate, sew up wrapping, obtain described mid distal femur Cranial defect model.
2. the construction method of mid distal femur Cranial defect model as claimed in claim 1, it is characterized in that, in described step (1), the animal of employing is rat, mice or rabbit.
3. the construction method of mid distal femur Cranial defect model as claimed in claim 2, it is characterized in that, described rat is SD rat.
4. the construction method of mid distal femur Cranial defect model as claimed in claim 1, it is characterized in that, in step (1), the described way appearing mid distal femur is: before lower in the femur of animal, otch is made in outside, cut skin, subcutaneous tissue and deep fascia, blunt separation, appears mid distal femur.
5. the construction method of mid distal femur Cranial defect model as claimed in claim 1, it is characterized in that, described blunt separation is specially: enter from the musculus lateralis externi gap blunt separation between vastus lateralis and biceps femoris.
6. the construction method of mid distal femur Cranial defect model as claimed in claim 1, is characterized in that, in step (2), after described manufacture Cranial defect, further comprising the steps of: to put into orthopedics biological material implant at Cranial defect position.
7. a mid distal femur Cranial defect model, it is characterized in that, described mid distal femur Cranial defect model builds by the following method: after (1) anesthetized animal, gets animal hind leg preserved skin, sterilization, before lower in the femur of animal, otch is made in outside, appears mid distal femur; (2) at the femur of mid distal femur excision different size, manufacture Cranial defect, irrigate, sew up wrapping, obtain described mid distal femur Cranial defect model.
8. the construction method of mid distal femur Cranial defect model as claimed in claim 1 is preparing the application in femur Cranial defect model.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107440686A (en) * | 2017-06-13 | 2017-12-08 | 菏泽学院 | One kind is used for mouse femoral bone impairment model and its multi-functional fixed system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107440686A (en) * | 2017-06-13 | 2017-12-08 | 菏泽学院 | One kind is used for mouse femoral bone impairment model and its multi-functional fixed system |
| CN107440686B (en) * | 2017-06-13 | 2020-08-04 | 菏泽学院 | Be used for damaged model of mouse thighbone and multi-functional fixing system thereof |
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