CN105211128A - A kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant and preparation method thereof - Google Patents
A kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant and preparation method thereof, this thimerosal is mainly made up of Chinese herbal medicine extract, multienzyme and auxiliary material; Described Chinese herbal medicine extract comprises: China tree bacterium, Hu Erlan, water vertebra wood, Caulis Lagenariae gourdae, clam leaf potato, three add the extract of flower, obtuselobed snakegourd leaf, florida waltheria root and stem, puckery grass, bitter Ao, wolf rake grass, guyan lophotocarpus herb, well limit madder, water chestnut stem, Chinese arborvitae root-bark, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi), vesicle grass, Japan-China flower, whitebracteole bugle herb, Folium Euphorbiae Antiquori, these Chinese medicine materials of vinegar woods; Described multienzyme is made up of bromelain, lipase, lactase, lysozyme.The present invention can not only effectively kill bacterium and virus, remaining bloodstain, purulence mark and secretion etc. on inspection apparatus thoroughly can also be disposed, to prevent secondary pollution, contained auxiliary material also has good antibacterial action, once cleaning just can meet the requirement of sterilization, without the need to carrying out secondary sterilization with special chlorination equipment again, cost-saving, raise the efficiency.
Description
Technical field
The present invention relates to technical field of medical examination, specifically relate to a kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant and preparation method thereof.
Background technology
Medical test is the Main Means judging disease, and be a very rigorous job, an error the breadth of a single hair can lead you a thousand li astray, therefore must ensure the accuracy of check data result.Whether the sterilizing checking apparatus thoroughly then can the accuracy of shadow assay greatly.
Medical worker is after analyzing and processing of testing, need inspection apparatus to be carried out unify sterilization cleaning, cleaning method in the past adopts manually to clean, there is new sterilization method afterwards, such as disinfection by ultraviolet light, microwave is sterilized, and tentatively cleaned inspection apparatus is placed in special device and carries out disinfection and store, but such sterilization method exists certain defect.Although the method adopting special device to carry out unifying to sterilize can kill Most bacterial and virus; but cannot remove residual bloodstain purulence mark and secretion etc. on inspection apparatus; the Proteinaceous organic matter cleaning that inspection apparatus pollutes is not thorough; to microorganism, there is protective effect; easily cause sterilizing failure; to take out after sterilization and can breed bacteria again, absolute sanitation and hygiene can not be ensured.Therefore, if can carry out disposablely cleaning thoroughly and sterilizing to inspection apparatus, cleaned up completely by the bloodstain etc. of remnants, sterilization thoroughly, then will not need again to sterilize.Greatly alleviate the work of staff, and the health of strict guarantee inspection apparatus.
Summary of the invention
The technical problem that the present invention solves is to provide a kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant and preparation method thereof, inspection apparatus can disposablely clean up by this Chinese medicine multienzyme compound disinfectant, sterilization thoroughly, prevents secondary pollution, ensures the accuracy of assay.
Technical scheme of the present invention is, a kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant, is mainly made up of Chinese herbal medicine extract, multienzyme and auxiliary material, described Chinese herbal medicine extract comprises by weight: China tree fungus extract 3.6 ~ 8.0 parts, the blue extract 5.5 ~ 10 parts of tiger ear, water vertebra wood extract 4.8 ~ 9.6 parts, Caulis Lagenariae gourdae extract 3.5 ~ 9.0 parts, clam leaf potato extract 2.8 ~ 8.4 parts, three add flower extract 2.4 ~ 8.0 parts, obtuselobed snakegourd leaf extract 2.6 ~ 8.5 parts, florida waltheria root and stem extract 3.2 ~ 4.6 parts, puckery careless extract 3.5 ~ 8.5 parts, Ku Ao extract 4.5 ~ 9.0 parts, wolf rake grass extract 4.4 ~ 10 parts, guyan lophotocarpus herb extract 3.1 ~ 3.5 parts, the alizarin extract 2.8 ~ 5.8 parts in well limit, water chestnut stem extraction 2.5 ~ 7.5 parts, Chinese arborvitae root-bark extract 3.0 ~ 9.0 parts, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi) extract 2.5 ~ 5.5 parts, vesicle grass extract 2.2 ~ 5.8 parts, Japan-China flower extract 2.0 ~ 6.4 parts, whitebracteole bugle herb extract 3.2 ~ 6.6 parts, Folium Euphorbiae Antiquori extract 1.8 ~ 6.4 parts, vinegar woods extract 1.5 ~ 4.5 parts, described multienzyme is made up of bromelain 3.0 ~ 5.2 parts, 1.5 ~ 3.5 parts, lipase, lactase 2.0 ~ 4.5 parts, lysozyme 3.4 ~ 4.8 parts by weight.
Further, described Chinese herbal medicine extract also comprises bigleaf fig resin extract 1.5 ~ 5.0 parts, Chinese apea-earring leaf extract 2.8 ~ 6.2 parts, colter point extract 3.3 ~ 7.5 parts.
Further, described Chinese herbal medicine extract also comprises a nettle extract 2.1 ~ 3.5 parts, straw sandals blue or green extract 1.5 ~ 4.5 parts, thatch melon leaf extract 1.4 ~ 2.6 parts.
Further, described Chinese herbal medicine extract also comprises phoenix bifurcation grass extract 2.6 ~ 5.4 parts, Herba Cynanchum decipies Schneid extract 1.2 ~ 3.5 parts, the blue extract 3.8 ~ 9.4 parts of Hua Wuzhu.
Further, described auxiliary material comprises by weight: Tween 80 6.2 ~ 10.5 parts, nutmeg sodium sarcosinate 3.5 ~ 8.5 parts, polyoxyethylenealkylphenol ether 2.4 ~ 5.0 parts, trichlorocarbanilide 1.8 ~ 4.4 parts, also comprise nano titanium oxide 0.5 ~ 1.3 part.
The preparation method of described a kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant, comprises the following steps:
(1) China tree bacterium is taken by described proportioning, be placed in fill 0.3 ~ 0.35% sodium bicarbonate solution supersonic wave cleaning machine in 100 ~ 180W, 33 ~ 36KHz cleans 15 ~ 30min, drain, vacuum microwave drying, put into pulverizing mill and be crushed to 40 ~ 50 orders, be placed in 4 ~ 8 times of water gagings to soak 2.5 ~ 10 hours, coolant-temperature gage is 50 ~ 55 DEG C, be cooled to normal temperature, add mixed solvent A to extract, described mixed solvent A is n-hexane, the mixed solution of ethyl acetate and acetone, mixed volume is than being n-hexane: ethyl acetate: acetone=(5 ~ 10): (2 ~ 6): (1 ~ 2), 1 ~ 2% fining agent is added in gained extract, centrifugal after clarifying treatment, obtain supernatant after centrifugal, be China tree bacterium extract, in a cell highly purified pair of silver electrode is immersed in China tree bacterium extract, by Electrode connection to power supply, and voltage is adjusted to 6 ~ 20V, electrolysis 4 ~ 30 minutes, after silver ion and China tree bacterium extract react, form dark-brown precipitate, remove this sediment completely by centrifugal or membrane filtration, obtain China tree bacterium silver ion extract, add the polysorbas20 of 0.1% ~ 0.5% and the propane diols of 45% again, after stirring and dissolving, be China tree fungus extract,
(2) Hu Erlan is taken respectively by described proportioning, water vertebra wood, Caulis Lagenariae gourdae, clam leaf potato, three add flower, obtuselobed snakegourd leaf, florida waltheria root and stem, puckery grass, Ku Ao, wolf rake grass, guyan lophotocarpus herb, well limit is alizarin, water chestnut stem, Chinese arborvitae root-bark, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi), vesicle grass, Japan-China flower, whitebracteole bugle herb, Folium Euphorbiae Antiquori, vinegar woods, bigleaf fig resin, Chinese apea-earring leaf, colter point, fiber crops, straw sandals are blue or green, thatch melon leaf, phoenix bifurcation grass, Herba Cynanchum decipies Schneid, Hua Wuzhu is blue, respectively the method for each medicinal material according to above-mentioned steps (1) process China tree bacterium is extracted, obtain the extract of each medicinal material, the extract of all medicinal materials is merged, be Chinese herbal medicine extract,
(3) take Tween 80, nutmeg sodium sarcosinate, polyoxyethylenealkylphenol ether, trichlorocarbanilide respectively by described proportioning, with water for injection in 23 ~ 25 DEG C of dissolvings, stir 3 ~ 5 hours, filter, collect filtrate; Chinese herbal medicine extract is added again in filtrate, stir 2 ~ 10 hours at 70 ~ 80 DEG C, speed is that 100 ~ 150rpm/ divides, by gained solution through centrifugal degassed, place 12 ~ 24 hours in 0 ~ 4 DEG C of low temperature, under this temperature condition, add multienzyme, stir 0.2 ~ 0.5 hour, speed is that 40 ~ 100rpm/ divides, packing, and sealing is preserved.
Further, described fining agent is the natural clarifying agent of ZTC1+1 II type, and remove the ability such as protein, tannin comparatively strong, settling velocity is fast, and cost is low, can retain active ingredient preferably.
Further, described storage temperature lower than 25 DEG C, to ensure the activity of enzyme.
The invention has the beneficial effects as follows, be made up of multiple Chinese herbal medicine extract and multienzyme, can not only effectively kill bacterium and virus, remaining bloodstain, purulence mark and secretion etc. on inspection apparatus thoroughly can also be disposed, to prevent secondary pollution, contained auxiliary material also has good antibacterial action, once cleaning just can meet the requirement of sterilization, without the need to carrying out secondary sterilization with special chlorination equipment again, cost-saving, raise the efficiency.
Embodiment
Embodiment 1:
A kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant, is mainly made up of Chinese herbal medicine extract, multienzyme and auxiliary material, described Chinese herbal medicine extract comprises by weight: China tree fungus extract 3.6 parts, the blue extract 5.5 parts of tiger ear, water vertebra wood extract 4.8 parts, Caulis Lagenariae gourdae extract 3.5 parts, clam leaf potato extract 2.8 parts, three add flower extract 2.4 parts, obtuselobed snakegourd leaf extract 2.6 parts, florida waltheria root and stem extract 3.2 parts, puckery careless extract 3.5 parts, Ku Ao extract 4.5 parts, wolf rake grass extract 4.4 parts, guyan lophotocarpus herb extract 3.1 parts, the alizarin extract 2.8 parts in well limit, water chestnut stem extraction 2.5 parts, Chinese arborvitae root-bark extract 3.0 parts, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi) extract 2.5 parts, vesicle grass extract 2.2 parts, Japan-China flower extract 2.0 parts, whitebracteole bugle herb extract 3.2 parts, Folium Euphorbiae Antiquori extract 1.8 parts, vinegar woods extract 1.5 parts, described multienzyme is made up of bromelain 3.0 parts, 1.5 parts, lipase, lactase 2.0 parts, lysozyme 3.4 parts by weight.Described auxiliary material comprises by weight: Tween 80 6.2 parts, nutmeg sodium sarcosinate 3.5 parts, polyoxyethylenealkylphenol ether 2.4 parts, trichlorocarbanilide 1.8 parts, also comprise nano titanium oxide 0.5 part.
The preparation method of described a kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant, comprises the following steps:
(1) China tree bacterium is taken by described proportioning, be placed in the supersonic wave cleaning machine filling 0.3% sodium bicarbonate solution and clean 15min in 100W, 33KHz, drain, vacuum microwave drying, put into pulverizing mill and be crushed to 40 orders, be placed in 4 times of water gagings to soak 2.5 hours, coolant-temperature gage is 50 DEG C, is cooled to normal temperature, adds mixed solvent A and extracts, described mixed solvent A is the mixed solution of n-hexane, ethyl acetate and acetone, and mixed volume is than being n-hexane: ethyl acetate: acetone=5:2:1; Add 1% fining agent in gained extract, described fining agent is the natural clarifying agent of ZTC1+1 II type, and remove the ability such as protein, tannin comparatively strong, settling velocity is fast, and cost is low, can retain active ingredient preferably; Centrifugal after clarifying treatment, supernatant is obtained after centrifugal, be China tree bacterium extract, in a cell highly purified pair of silver electrode immersed in China tree bacterium extract, by Electrode connection to power supply, and voltage is adjusted to 6V, electrolysis 4 minutes, after silver ion and China tree bacterium extract react, forms dark-brown precipitate, remove this sediment completely by centrifugal or membrane filtration, obtain China tree bacterium silver ion extract; Add the polysorbas20 of 0.1% and the propane diols of 45% again, after stirring and dissolving, be China tree fungus extract;
(2) take Hu Erlan respectively by described proportioning, water vertebra wood, Caulis Lagenariae gourdae, clam leaf potato, three add flower, obtuselobed snakegourd leaf, florida waltheria root and stem, puckery grass, bitter Ao, wolf rake grass, guyan lophotocarpus herb, well limit madder, water chestnut stem, Chinese arborvitae root-bark, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi), vesicle grass, Japan-China flower, whitebracteole bugle herb, Folium Euphorbiae Antiquori, vinegar woods, respectively the method for each medicinal material according to above-mentioned steps (1) process China tree bacterium is extracted, obtain the extract of each medicinal material, the extract of all medicinal materials is merged, is Chinese herbal medicine extract;
(3) take Tween 80, nutmeg sodium sarcosinate, polyoxyethylenealkylphenol ether, trichlorocarbanilide respectively by described proportioning, with water for injection in 23 DEG C of dissolvings, stir 3 hours, filter, collect filtrate; Chinese herbal medicine extract is added again in filtrate, stir 2 hours at 70 DEG C, speed is that 100rpm/ divides, by gained solution through centrifugal degassed, 12 hours are placed in 0 DEG C of low temperature, under this temperature condition, add multienzyme, stir 0.2 hour, speed is that 40rpm/ divides, packing, sealing preserve, storage temperature lower than 25 DEG C, to ensure the activity of enzyme.
Embodiment 2:
A kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant, is mainly made up of Chinese herbal medicine extract, multienzyme and auxiliary material, described Chinese herbal medicine extract comprises by weight: China tree fungus extract 4.8 parts, the blue extract 7.75 parts of tiger ear, water vertebra wood extract 7.2 parts, Caulis Lagenariae gourdae extract 6.25 parts, clam leaf potato extract 5.6 parts, three add flower extract 5.2 parts, obtuselobed snakegourd leaf extract 5.55 parts, florida waltheria root and stem extract 3.9 parts, puckery careless extract 6 parts, Ku Ao extract 6.75 parts, wolf rake grass extract 7.2 parts, guyan lophotocarpus herb extract 3.3 parts, the alizarin extract 4.3 parts in well limit, water chestnut stem extraction 5 parts, Chinese arborvitae root-bark extract 6.0 parts, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi) extract 4 parts, vesicle grass extract 4 parts, Japan-China flower extract 4.2 parts, whitebracteole bugle herb extract 4.9 parts, Folium Euphorbiae Antiquori extract 4.1 parts, vinegar woods extract 3 parts, bigleaf fig resin extract 3.25 parts, Chinese apea-earring leaf extract 4.5 parts, colter point extract 5.4 parts, described multienzyme is made up of bromelain 4.1 parts, 2.5 parts, lipase, lactase 3.25 parts, lysozyme 4.1 parts by weight, described auxiliary material comprises by weight: Tween 80 7.85 parts, nutmeg sodium sarcosinate 6 parts, polyoxyethylenealkylphenol ether 3.7 parts, trichlorocarbanilide 3.1 parts, also comprise nano titanium oxide 0.9 part.
The preparation method of described a kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant, comprises the following steps:
(1) China tree bacterium is taken by described proportioning, be placed in the supersonic wave cleaning machine filling 0.325% sodium bicarbonate solution and clean 22.5min in 140W, 34.5KHz, drain, vacuum microwave drying, put into pulverizing mill and be crushed to 45 orders, be placed in 6 times of water gagings to soak 6.25 hours, coolant-temperature gage is 52.5 DEG C, be cooled to normal temperature, add mixed solvent A to extract, described mixed solvent A is the mixed solution of n-hexane, ethyl acetate and acetone, and mixed volume is than being n-hexane: ethyl acetate: acetone=7.5:4:1.5; Add 1.5% fining agent in gained extract, described fining agent is the natural clarifying agent of ZTC1+1 II type, and remove the ability such as protein, tannin comparatively strong, settling velocity is fast, and cost is low, can retain active ingredient preferably; Centrifugal after clarifying treatment, supernatant is obtained after centrifugal, be China tree bacterium extract, in a cell highly purified pair of silver electrode immersed in China tree bacterium extract, by Electrode connection to power supply, and voltage is adjusted to 13V, electrolysis 17 minutes, after silver ion and China tree bacterium extract react, forms dark-brown precipitate, remove this sediment completely by centrifugal or membrane filtration, obtain China tree bacterium silver ion extract; Add the polysorbas20 of 0.3% and the propane diols of 45% again, after stirring and dissolving, be China tree fungus extract;
(2) take Hu Erlan respectively by described proportioning, water vertebra wood, Caulis Lagenariae gourdae, clam leaf potato, three add flower, obtuselobed snakegourd leaf, florida waltheria root and stem, puckery grass, bitter Ao, wolf rake grass, guyan lophotocarpus herb, well limit madder, water chestnut stem, Chinese arborvitae root-bark, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi), vesicle grass, Japan-China flower, whitebracteole bugle herb, Folium Euphorbiae Antiquori, vinegar woods, bigleaf fig resin, Chinese apea-earring leaf, colter be sharp, respectively the method for each medicinal material according to above-mentioned steps (1) process China tree bacterium is extracted, obtain the extract of each medicinal material, the extract of all medicinal materials is merged, is Chinese herbal medicine extract;
(3) take Tween 80, nutmeg sodium sarcosinate, polyoxyethylenealkylphenol ether, trichlorocarbanilide respectively by described proportioning, with water for injection in 24 DEG C of dissolvings, stir 4 hours, filter, collect filtrate; Chinese herbal medicine extract is added again in filtrate, stir 6 hours at 75 DEG C, speed is that 125rpm/ divides, by gained solution through centrifugal degassed, 18 hours are placed in 2 DEG C of low temperature, under this temperature condition, add multienzyme, stir 0.35 hour, speed is that 70rpm/ divides, packing, sealing preserve, storage temperature lower than 25 DEG C, to ensure the activity of enzyme.
Embodiment 3:
A kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant, is mainly made up of Chinese herbal medicine extract, multienzyme and auxiliary material, described Chinese herbal medicine extract comprises by weight: China tree fungus extract 8.0 parts, the blue extract 10 parts of tiger ear, water vertebra wood extract 9.6 parts, Caulis Lagenariae gourdae extract 9.0 parts, clam leaf potato extract 8.4 parts, three add flower extract 8.0 parts, obtuselobed snakegourd leaf extract 8.5 parts, florida waltheria root and stem extract 4.6 parts, puckery careless extract 8.5 parts, Ku Ao extract 9.0 parts, wolf rake grass extract 10 parts, guyan lophotocarpus herb extract 3.5 parts, the alizarin extract 5.8 parts in well limit, water chestnut stem extraction 7.5 parts, Chinese arborvitae root-bark extract 9.0 parts, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi) extract 5.5 parts, vesicle grass extract 5.8 parts, Japan-China flower extract 6.4 parts, whitebracteole bugle herb extract 6.6 parts, Folium Euphorbiae Antiquori extract 6.4 parts, vinegar woods extract 4.5 parts, bigleaf fig resin extract 5.0 parts, Chinese apea-earring leaf extract 6.2 parts, colter point extract 7.5 parts, nettle extract 3.5 parts, the blue or green extract 4.5 parts of straw sandals, thatch melon leaf extract 2.6 parts, described multienzyme is made up of bromelain 5.2 parts, 3.5 parts, lipase, lactase 4.5 parts, lysozyme 4.8 parts by weight, described auxiliary material comprises by weight: Tween 80 10.5 parts, nutmeg sodium sarcosinate 8.5 parts, polyoxyethylenealkylphenol ether 5.0 parts, trichlorocarbanilide 4.4 parts, also comprise nano titanium oxide 1.3 parts.
The preparation method of described a kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant, comprises the following steps:
(1) China tree bacterium is taken by described proportioning, be placed in the supersonic wave cleaning machine filling 0.35% sodium bicarbonate solution and clean 30min in 180W, 36KHz, drain, vacuum microwave drying, put into pulverizing mill and be crushed to 50 orders, be placed in 8 times of water gagings to soak 10 hours, coolant-temperature gage is 55 DEG C, is cooled to normal temperature, adds mixed solvent A and extracts, described mixed solvent A is the mixed solution of n-hexane, ethyl acetate and acetone, and mixed volume is than being n-hexane: ethyl acetate: acetone=10:6:2; Add 2% fining agent in gained extract, described fining agent is the natural clarifying agent of ZTC1+1 II type, and remove the ability such as protein, tannin comparatively strong, settling velocity is fast, and cost is low, can retain active ingredient preferably; Centrifugal after clarifying treatment, supernatant is obtained after centrifugal, be China tree bacterium extract, in a cell highly purified pair of silver electrode immersed in China tree bacterium extract, by Electrode connection to power supply, and voltage is adjusted to 20V, electrolysis 30 minutes, after silver ion and China tree bacterium extract react, forms dark-brown precipitate, remove this sediment completely by centrifugal or membrane filtration, obtain China tree bacterium silver ion extract; Add the polysorbas20 of 00.5% and the propane diols of 45% again, after stirring and dissolving, be China tree fungus extract;
(2) Hu Erlan is taken respectively by described proportioning, water vertebra wood, Caulis Lagenariae gourdae, clam leaf potato, three add flower, obtuselobed snakegourd leaf, florida waltheria root and stem, puckery grass, Ku Ao, wolf rake grass, guyan lophotocarpus herb, well limit is alizarin, water chestnut stem, Chinese arborvitae root-bark, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi), vesicle grass, Japan-China flower, whitebracteole bugle herb, Folium Euphorbiae Antiquori, vinegar woods, bigleaf fig resin, Chinese apea-earring leaf, colter point, fiber crops, straw sandals are blue or green, thatch melon leaf, respectively the method for each medicinal material according to above-mentioned steps (1) process China tree bacterium is extracted, obtain the extract of each medicinal material, the extract of all medicinal materials is merged, be Chinese herbal medicine extract,
(3) take Tween 80, nutmeg sodium sarcosinate, polyoxyethylenealkylphenol ether, trichlorocarbanilide respectively by described proportioning, with water for injection in 25 DEG C of dissolvings, stir 5 hours, filter, collect filtrate; Chinese herbal medicine extract is added again in filtrate, stir 10 hours at 80 DEG C, speed is that 150rpm/ divides, by gained solution through centrifugal degassed, 24 hours are placed in 4 DEG C of low temperature, under this temperature condition, add multienzyme, stir 0.5 hour, speed is that 100rpm/ divides, packing, sealing preserve, storage temperature lower than 25 DEG C, to ensure the activity of enzyme.
Embodiment 4:
A kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant, is mainly made up of Chinese herbal medicine extract, multienzyme and auxiliary material, described Chinese herbal medicine extract comprises by weight: China tree fungus extract 8.0 parts, the blue extract 10 parts of tiger ear, water vertebra wood extract 9.6 parts, Caulis Lagenariae gourdae extract 9.0 parts, clam leaf potato extract 8.4 parts, three add flower extract 8.0 parts, obtuselobed snakegourd leaf extract 8.5 parts, florida waltheria root and stem extract 4.6 parts, puckery careless extract 8.5 parts, Ku Ao extract 9.0 parts, wolf rake grass extract 10 parts, guyan lophotocarpus herb extract 3.5 parts, the alizarin extract 5.8 parts in well limit, water chestnut stem extraction 7.5 parts, Chinese arborvitae root-bark extract 9.0 parts, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi) extract 5.5 parts, vesicle grass extract 5.8 parts, Japan-China flower extract 6.4 parts, whitebracteole bugle herb extract 6.6 parts, Folium Euphorbiae Antiquori extract 6.4 parts, vinegar woods extract 4.5 parts, bigleaf fig resin extract 5.0 parts, Chinese apea-earring leaf extract 6.2 parts, colter point extract 7.5 parts, nettle extract 3.5 parts, the blue or green extract 4.5 parts of straw sandals, thatch melon leaf extract 2.6 parts, phoenix bifurcation grass extract 5.4 parts, Herba Cynanchum decipies Schneid extract 3.5 parts, the blue extract 9.4 parts of Hua Wuzhu, described multienzyme is made up of bromelain 5.2 parts, 3.5 parts, lipase, lactase 4.5 parts, lysozyme 4.8 parts by weight, described auxiliary material comprises by weight: Tween 80 10.5 parts, nutmeg sodium sarcosinate 8.5 parts, polyoxyethylenealkylphenol ether 5.0 parts, trichlorocarbanilide 4.4 parts, also comprise nano titanium oxide 1.3 parts.
The preparation method of described a kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant, comprises the following steps:
(1) China tree bacterium is taken by described proportioning, be placed in the supersonic wave cleaning machine filling 0.35% sodium bicarbonate solution and clean 130min in 180W, 36KHz, drain, vacuum microwave drying, put into pulverizing mill and be crushed to 50 orders, be placed in 8 times of water gagings to soak 10 hours, coolant-temperature gage is 55 DEG C, is cooled to normal temperature, adds mixed solvent A and extracts, described mixed solvent A is the mixed solution of n-hexane, ethyl acetate and acetone, and mixed volume is than being n-hexane: ethyl acetate: acetone=10:6:2; Add 2% fining agent in gained extract, described fining agent is the natural clarifying agent of ZTC1+1 II type, and remove the ability such as protein, tannin comparatively strong, settling velocity is fast, and cost is low, can retain active ingredient preferably; Centrifugal after clarifying treatment, supernatant is obtained after centrifugal, be China tree bacterium extract, in a cell highly purified pair of silver electrode immersed in China tree bacterium extract, by Electrode connection to power supply, and voltage is adjusted to 20V, electrolysis 30 minutes, after silver ion and China tree bacterium extract react, forms dark-brown precipitate, remove this sediment completely by centrifugal or membrane filtration, obtain China tree bacterium silver ion extract; Add the polysorbas20 of 0.5% and the propane diols of 45% again, after stirring and dissolving, be China tree fungus extract;
(2) Hu Erlan is taken respectively by described proportioning, water vertebra wood, Caulis Lagenariae gourdae, clam leaf potato, three add flower, obtuselobed snakegourd leaf, florida waltheria root and stem, puckery grass, Ku Ao, wolf rake grass, guyan lophotocarpus herb, well limit is alizarin, water chestnut stem, Chinese arborvitae root-bark, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi), vesicle grass, Japan-China flower, whitebracteole bugle herb, Folium Euphorbiae Antiquori, vinegar woods, bigleaf fig resin, Chinese apea-earring leaf, colter point, fiber crops, straw sandals are blue or green, thatch melon leaf, phoenix bifurcation grass, Herba Cynanchum decipies Schneid, Hua Wuzhu is blue, respectively the method for each medicinal material according to above-mentioned steps (1) process China tree bacterium is extracted, obtain the extract of each medicinal material, the extract of all medicinal materials is merged, be Chinese herbal medicine extract,
(3) take Tween 80, nutmeg sodium sarcosinate, polyoxyethylenealkylphenol ether, trichlorocarbanilide respectively by described proportioning, with water for injection in 25 DEG C of dissolvings, stir 5 hours, filter, collect filtrate; Chinese herbal medicine extract is added again in filtrate, stir 10 hours at 80 DEG C, speed is that 150rpm/ divides, by gained solution through centrifugal degassed, 24 hours are placed in 4 DEG C of low temperature, under this temperature condition, add multienzyme, stir 0.5 hour, speed is that 100rpm/ divides, packing, sealing preserve, storage temperature lower than 25 DEG C, to ensure the activity of enzyme.
Sterilization experiment:
1. experimental strain: Escherichia coli, staphylococcus aureus, Pseudomonas aeruginosa, bacillus subtilis, is designated as I, II, III, IV respectively.
2. bacterial culture: be inoculated in beef-protein medium respectively by above-mentioned bacterial classification and cultivate, cultivates 24 hours under the temperature condition of 37 DEG C.
3. experimental technique: with thimerosal of the present invention as experimental group, with Normal Saline as a control group, respectively get 50ml, be placed in sterile petri dish; By bacterium cultured in culture dish with a little thalline of oese picking in the test tube that stroke-physiological saline solution is housed, concussion, formed bacteria suspension; Obtained bacteria suspension 0.2ml is placed in respectively the culture dish of experimental group and control group, mixes; Observe the bactericidal effect of two groups, calculate and statistics, sterilizing rate computational methods: sterilizing rate=(total clump count-residue clump count)/total clump count * 100%, result is as shown in table 1:
Toxicity test:
Test method: get 10 healthy adult rats and 10 healthy young mouse, shave off the hair of mao belly respectively, shave the circle that mao area size is diameter 3.5cm, Chinese medicine multienzyme compound disinfectant of the present invention is smeared and the region of shaving hair, rat applying amount is 0.6g/kg, children mouse applying amount is 0.5g/kg, cover with gauze and seal wrapping, pull down gauze week about again again to smear, consumption is identical with first time consumption, and observe the 1st week, 2 weeks, 3 weeks, the growing state of 4 weeks, experimental session diet drinking-water is all normal, do not show any abnormalities reaction, without the poisoning and phenomena of mortality, gauze inspection is taken apart after 4 weeks, application area do not occur any rot etc. symptom, equally excellent with normal skin.Visible, Chinese medicine multienzyme compound disinfectant of the present invention is nontoxic for rat and young mouse.
Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in previous embodiment, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (8)
1. clinical laboratory's Chinese medicine special multienzyme compound disinfectant, is characterized in that, is mainly made up of Chinese herbal medicine extract, multienzyme and auxiliary material, described Chinese herbal medicine extract comprises by weight: China tree fungus extract 3.6 ~ 8.0 parts, the blue extract 5.5 ~ 10 parts of tiger ear, water vertebra wood extract 4.8 ~ 9.6 parts, Caulis Lagenariae gourdae extract 3.5 ~ 9.0 parts, clam leaf potato extract 2.8 ~ 8.4 parts, three add flower extract 2.4 ~ 8.0 parts, obtuselobed snakegourd leaf extract 2.6 ~ 8.5 parts, florida waltheria root and stem extract 3.2 ~ 4.6 parts, puckery careless extract 3.5 ~ 8.5 parts, Ku Ao extract 4.5 ~ 9.0 parts, wolf rake grass extract 4.4 ~ 10 parts, guyan lophotocarpus herb extract 3.1 ~ 3.5 parts, the alizarin extract 2.8 ~ 5.8 parts in well limit, water chestnut stem extraction 2.5 ~ 7.5 parts, Chinese arborvitae root-bark extract 3.0 ~ 9.0 parts, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi) extract 2.5 ~ 5.5 parts, vesicle grass extract 2.2 ~ 5.8 parts, Japan-China flower extract 2.0 ~ 6.4 parts, whitebracteole bugle herb extract 3.2 ~ 6.6 parts, Folium Euphorbiae Antiquori extract 1.8 ~ 6.4 parts, vinegar woods extract 1.5 ~ 4.5 parts, described multienzyme is made up of bromelain 3.0 ~ 5.2 parts, 1.5 ~ 3.5 parts, lipase, lactase 2.0 ~ 4.5 parts, lysozyme 3.4 ~ 4.8 parts by weight.
2. a kind of clinical laboratory as claimed in claim 1 Chinese medicine special multienzyme compound disinfectant, is characterized in that, described Chinese herbal medicine extract also comprises bigleaf fig resin extract 1.5 ~ 5.0 parts, Chinese apea-earring leaf extract 2.8 ~ 6.2 parts, colter point extract 3.3 ~ 7.5 parts.
3. a kind of clinical laboratory as claimed in claim 2 Chinese medicine special multienzyme compound disinfectant, is characterized in that, described Chinese herbal medicine extract also comprises a nettle extract 2.1 ~ 3.5 parts, straw sandals blue or green extract 1.5 ~ 4.5 parts, thatch melon leaf extract 1.4 ~ 2.6 parts.
4. a kind of clinical laboratory as claimed in claim 3 Chinese medicine special multienzyme compound disinfectant, is characterized in that, described Chinese herbal medicine extract also comprises phoenix bifurcation grass extract 2.6 ~ 5.4 parts, Herba Cynanchum decipies Schneid extract 1.2 ~ 3.5 parts, the blue extract 3.8 ~ 9.4 parts of Hua Wuzhu.
5. a kind of clinical laboratory as claimed in claim 1 Chinese medicine special multienzyme compound disinfectant, it is characterized in that, described auxiliary material comprises by weight: Tween 80 6.2 ~ 10.5 parts, nutmeg sodium sarcosinate 3.5 ~ 8.5 parts, polyoxyethylenealkylphenol ether 2.4 ~ 5.0 parts, trichlorocarbanilide 1.8 ~ 4.4 parts.
6. the preparation method of a kind of clinical laboratory Chinese medicine special multienzyme compound disinfectant as described in Claims 1-4 any one, is characterized in that, comprise the following steps:
(1) China tree bacterium is taken by described proportioning, be placed in fill 0.3 ~ 0.35% sodium bicarbonate solution supersonic wave cleaning machine in 100 ~ 180W, 33 ~ 36KHz cleans 15 ~ 30min, drain, vacuum microwave drying, put into pulverizing mill and be crushed to 40 ~ 50 orders, be placed in 4 ~ 8 times of water gagings to soak 2.5 ~ 10 hours, coolant-temperature gage is 50 ~ 55 DEG C, be cooled to normal temperature, add mixed solvent A to extract, described mixed solvent A is n-hexane, the mixed solution of ethyl acetate and acetone, mixed volume is than being n-hexane: ethyl acetate: acetone=(5 ~ 10): (2 ~ 6): (1 ~ 2), 1 ~ 2% fining agent is added in gained extract, centrifugal after clarifying treatment, obtain supernatant after centrifugal, be China tree bacterium extract, in a cell highly purified pair of silver electrode is immersed in China tree bacterium extract, by Electrode connection to power supply, and voltage is adjusted to 6 ~ 20V, electrolysis 4 ~ 30 minutes, after silver ion and China tree bacterium extract react, form dark-brown precipitate, remove this sediment completely by centrifugal or membrane filtration, obtain China tree bacterium silver ion extract, add the polysorbas20 of 0.1% ~ 0.5% and the propane diols of 45% again, after stirring and dissolving, be China tree fungus extract,
(2) Hu Erlan is taken respectively by described proportioning, water vertebra wood, Caulis Lagenariae gourdae, clam leaf potato, three add flower, obtuselobed snakegourd leaf, florida waltheria root and stem, puckery grass, Ku Ao, wolf rake grass, guyan lophotocarpus herb, well limit is alizarin, water chestnut stem, Chinese arborvitae root-bark, Herba seu Radix Amaranrhi (Radix Seu Herba Amaranthi Spinosi), vesicle grass, Japan-China flower, whitebracteole bugle herb, Folium Euphorbiae Antiquori, vinegar woods, bigleaf fig resin, Chinese apea-earring leaf, colter point, fiber crops, straw sandals are blue or green, thatch melon leaf, phoenix bifurcation grass, Herba Cynanchum decipies Schneid, Hua Wuzhu is blue, respectively the method for each medicinal material according to above-mentioned steps (1) process China tree bacterium is extracted, obtain the extract of each medicinal material, the extract of all medicinal materials is merged, be Chinese herbal medicine extract,
(3) take Tween 80, nutmeg sodium sarcosinate, polyoxyethylenealkylphenol ether, trichlorocarbanilide respectively by described proportioning, with water for injection in 23 ~ 25 DEG C of dissolvings, stir 3 ~ 5 hours, filter, collect filtrate; Chinese herbal medicine extract is added again in filtrate, stir 2 ~ 10 hours at 70 ~ 80 DEG C, speed is that 100 ~ 150rpm/ divides, by gained solution through centrifugal degassed, place 12 ~ 24 hours in 0 ~ 4 DEG C of low temperature, under this temperature condition, add multienzyme, stir 0.2 ~ 0.5 hour, speed is that 40 ~ 100rpm/ divides, packing, and sealing is preserved.
7. the preparation method of a kind of clinical laboratory as claimed in claim 6 Chinese medicine special multienzyme compound disinfectant, it is characterized in that, described fining agent is the natural clarifying agent of ZTC1+1 II type.
8. the preparation method of a kind of clinical laboratory as claimed in claim 6 Chinese medicine special multienzyme compound disinfectant, it is characterized in that, described storage temperature is lower than 25 DEG C.
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