CN105210872A - A kind of drop of water rhizome seedling-cultivating method - Google Patents
A kind of drop of water rhizome seedling-cultivating method Download PDFInfo
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- CN105210872A CN105210872A CN201510666787.XA CN201510666787A CN105210872A CN 105210872 A CN105210872 A CN 105210872A CN 201510666787 A CN201510666787 A CN 201510666787A CN 105210872 A CN105210872 A CN 105210872A
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Abstract
The present invention relates to botanical seedling culturing technical field, be specifically related to a kind of drop of water rhizome seedling-cultivating method.Get the fresh rhizome of wild drop of water, its surperficial earth is rinsed well, after being cut into bulk, 24-28 hour is soaked in D-Fructose solution, block rhizome is placed in flowing water and rinses well, block rhizome is placed in aseptic culture medium, 2-3 days is cultivated at 15-18 DEG C, block rhizome is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 20-22 DEG C, illumination is 18-20klx, root media is by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, vermiculite power 20 parts, 500 parts, water is mixed.
Description
Technical field
The present invention relates to botanical seedling culturing technical field, be specifically related to a kind of drop of water rhizome seedling-cultivating method.
Background technology
Drop of water belongs to Araeceae draft, medicinal underground bulb, have eliminating dampness and eliminating phlegm, effect of dissolving lump and resolving mass, to the wet phlegm cold drink for the treatment of, vomiting, gastric disorder causing nausea, cough, phlegm is many, headache egersis etc. has good effect, this product consumption is large, also be export large kind throughout the year, Ju is national Ge great Yao city and the analysis of producing region market investigation statistics over 10 years, drought tuber of pinellia supply demand gap is larger, tendency is powerful, drop of water happiness shade environment, in recent years reclamation of wasteland deforestation, a large amount of use herbicide, that its growing environment goes to pot and causes wild area to reduce year by year, output falls sharply thereupon, and artificial planting is also because planting cost is high, the reasons such as miscarriage, output is always lower.
Summary of the invention
The object of the present invention is to provide a kind of drop of water rhizome seedling-cultivating method.
A kind of drop of water rhizome seedling-cultivating method, it comprises the steps:
1), get the fresh rhizome of wild drop of water, its surperficial earth rinsed well, after being cut into bulk, concentration be 800ppm, temperature is soak 24-28 hour in the D-Fructose solution of 10-15 DEG C;
2), block rhizome is placed in flowing water rinses well;
3), block rhizome is placed in aseptic culture medium, 2-3 days is cultivated at 15-18 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, distilled water 1000 parts;
4), block rhizome is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 20-22 DEG C, illumination is 18-20klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, vermiculite power 20 parts, 500 parts, water.
Rhizome seedling-cultivating method of the present invention can reduce the content of ABA in rhizome fast, breaking dormancy effect, promotes the fast healing and taking root of drop of water rhizome, improves rooting rate, cultivate in root media and can grow healthy and strong root system in 20 days, without the need to can directly transplant to land for growing field crops through hardening after taking root, emerge after transplanting fast, emergence rate be high, emergence rate can reach 93.75%, the standardized quick fast-growing that can realize drop of water seedling is produced, and shortens crop cycle, increases economic efficiency.
Embodiment
Embodiment 1,1, get the fresh rhizome of wild drop of water, its surperficial earth is rinsed well, after being cut into bulk, concentration be 800ppm, temperature is soak 24 hours in the D-Fructose solution of 10 DEG C; 2, block rhizome is placed in flowing water rinses well; 3, be placed in aseptic culture medium by block rhizome, cultivate 2 days at 15 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, distilled water 1000 parts; 4, block rhizome is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 20 DEG C, illumination is 18klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, vermiculite power 20 parts, 500 parts, water.
Obtain block rhizome average cultivation in root media can take root for 20 days, rooting rate can reach 98.11%, transplants to land for growing field crops after taking root, and adds up survival rate after 3 weeks, and survival rate can reach 92.13%.
Embodiment 2,1, get the fresh rhizome of wild drop of water, its surperficial earth is rinsed well, after being cut into bulk, concentration be 800ppm, temperature is soak 28 hours in the D-Fructose solution of 15 DEG C; 2, block rhizome is placed in flowing water rinses well; 3, be placed in aseptic culture medium by block rhizome, cultivate 3 days at 18 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, distilled water 1000 parts; 4, block rhizome is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 22 DEG C, illumination is 20klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, vermiculite power 20 parts, 500 parts, water.
Obtain block rhizome average cultivation in root media can take root for 18 days, rooting rate can reach 98.96%, transplants to land for growing field crops after taking root, and adds up survival rate after 3 weeks, and survival rate can reach 93.75%.
Embodiment 3,1, get the fresh rhizome of wild drop of water, its surperficial earth is rinsed well, after being cut into bulk, concentration be 800ppm, temperature is soak 25 hours in the D-Fructose solution of 13 DEG C; 2, block rhizome is placed in flowing water rinses well; 3, be placed in aseptic culture medium by block rhizome, cultivate 3 days at 16 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, distilled water 1000 parts; 4, block rhizome is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 21 DEG C, illumination is 19klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, vermiculite power 20 parts, 500 parts, water.
Obtain block rhizome average cultivation in root media can take root for 19.3 days, rooting rate can reach 98.35%, transplants to land for growing field crops after taking root, and adds up survival rate after 3 weeks, and survival rate can reach 92.79%.
Claims (1)
1. a drop of water rhizome seedling-cultivating method, is characterized in that, it comprises the steps:
1), get the fresh rhizome of wild drop of water, its surperficial earth rinsed well, after being cut into bulk, concentration be 800ppm, temperature is soak 24-28 hour in the D-Fructose solution of 10-15 DEG C;
2), block rhizome is placed in flowing water rinses well;
3), block rhizome is placed in aseptic culture medium, 2-3 days is cultivated at 15-18 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, distilled water 1000 parts;
4), block rhizome is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 20-22 DEG C, illumination is 18-20klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, vermiculite power 20 parts, 500 parts, water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510666787.XA CN105210872A (en) | 2015-10-16 | 2015-10-16 | A kind of drop of water rhizome seedling-cultivating method |
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| CN201510666787.XA CN105210872A (en) | 2015-10-16 | 2015-10-16 | A kind of drop of water rhizome seedling-cultivating method |
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| CN105210872A true CN105210872A (en) | 2016-01-06 |
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| CN201510666787.XA Pending CN105210872A (en) | 2015-10-16 | 2015-10-16 | A kind of drop of water rhizome seedling-cultivating method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1602678A (en) * | 2004-11-04 | 2005-04-06 | 浙江省农业科学院 | Quick reproduction method for cordate halfsummer tissue culture |
| CN101803571A (en) * | 2010-03-30 | 2010-08-18 | 浙江理工大学 | Tissue culture rapid propagation method of Rhizoma Typhonii Flagelliformis |
| CN102150624A (en) * | 2011-04-29 | 2011-08-17 | 南京工业大学 | Tissue culture and rapid propagation method for pinellia tuber plant |
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2015
- 2015-10-16 CN CN201510666787.XA patent/CN105210872A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1602678A (en) * | 2004-11-04 | 2005-04-06 | 浙江省农业科学院 | Quick reproduction method for cordate halfsummer tissue culture |
| CN101803571A (en) * | 2010-03-30 | 2010-08-18 | 浙江理工大学 | Tissue culture rapid propagation method of Rhizoma Typhonii Flagelliformis |
| CN102150624A (en) * | 2011-04-29 | 2011-08-17 | 南京工业大学 | Tissue culture and rapid propagation method for pinellia tuber plant |
Non-Patent Citations (1)
| Title |
|---|
| 郁建新: "滴水珠的组织培养和植株再生", 《植物生理学通讯》 * |
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Application publication date: 20160106 |
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