CN105199014A - Preparation method for extracting heparin sodium from animal waste materials - Google Patents
Preparation method for extracting heparin sodium from animal waste materials Download PDFInfo
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- CN105199014A CN105199014A CN201510720913.5A CN201510720913A CN105199014A CN 105199014 A CN105199014 A CN 105199014A CN 201510720913 A CN201510720913 A CN 201510720913A CN 105199014 A CN105199014 A CN 105199014A
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- glue
- heparin sodium
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- animal waste
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Abstract
The invention discloses a preparation method for extracting heparin sodium from animal waste materials. The preparation method comprises the following steps: cleaning: offcut of a natural casing, bone glue, isinglass or hoof glue is cleaned repeatedly with clear water for 3-5 times; glue liquid extraction: the cleaned offcut, bone glue, isinglass or hoof glue is placed into a high-temperature pot for dissolution, resin is added after dissolution, and a glue liquid is extracted; elution: 2709 basic protease is added to water obtained through exchange separation, then saline water in the mass fraction of 3%-4% is added, stirring is performed at the certain temperature, elution is performed, and an eluent is extracted; precipitation: ethanol in the mass fraction of 70%-80% is added to the eluent with stirring, the mixture is stirred uniformly and sealed for precipitation, and a precipitate is collected; drying: the precipitate is placed into a drying oven for drying, and heparin sodium can be obtained. The preparation method for extracting heparin sodium from the animal waste materials has the advantages as follows: the preparation process is simple, operation is convenient, heparin sodium is extracted effectively through treatment of the offcut, utilization of the offcut of the casing is greatly improved, and unnecessary waste is avoided.
Description
Technical field
The invention belongs to casing production technical field, be specifically related to the preparation method that a kind of animal waste extracts heparin sodium.
Background technology
Heparin sodium is mucopolysaccharide sulfuric acid ester anticoagulant, it is the sodium salt of the CSSO3 extracted from the intestinal mucosa of pig or ox, belong to mucopolysaccharide material, naturally be present in mastocyte, there is the effect of reducing blood-fat, be widely used in treating artery and vein thrombus and pulmonary infarction, cerebral infarction, unstable angina pectoris, Acute Myocardial Infarction etc.
The intestines skin of chitterlings, casing, intestinal mucosa contain abundant heparin sodium, the production idea that traditional natural casing makes, the starting point simply stare on intestinal mucosa, have ignored and heparin sodium contained in intestines skin, casing is extracted, in process, due to the reason of machinery equipment, the casing produced generally has fixing length, but the casing length that user needs is not fixed, different producers requires also different, and this produces a large amount of rim charges with regard to unavoidable in cutting casing process.The a large amount of rim charges in the past produced, or be discarded, waste resource, or adopt the method for artificial overlap joint to make the casing of sl..The rim charge of casing is reclaimed by the method for overlap joint, although also utilize the resource of waste, but due to artificial overlap joint, the overlap of part casing must be had, and cause whole casing gage distortion, be uneven, and owing to being that remaining rim charge is reused with overlapping mode, the structure of overlap joint is different in size unavoidably, is unfavorable for unified processing.In addition, will form qualified casing after overlap joint, also need to dry under the sun, this just receives the restriction of weather to a certain extent.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, a kind of preparation method that can effectively utilize the animal waste of casing rim charge to extract heparin sodium is now provided.
For achieving the above object, technical scheme of the present invention is: a kind of animal waste extracts the preparation method of heparin sodium, and its innovative point is: through cleaning, extract the preparation that glue, wash-out, precipitation and drying step complete heparin sodium; Concrete steps are as follows:
(1) clean: repeatedly clean under the rim charge of natural casing, gelatine, fish glue or hoof glue are put into clear water, described wash number is 3-5 time;
(2) extract glue: the rim charge after above-mentioned cleaning, gelatine, fish glue or hoof glue are put into high temperature pot and dissolves, add resins exchange after dissolving and extract glue;
(3) wash-out: is added 2709 appropriate Sumizyme MPs in the water that exchange is separated, regulate PH to 8-9, and then add the salt solution that massfraction is 3-4%, stir at a certain temperature, described churning time is 1-2 hour, carries out wash-out, extracts elutriant;
(4) precipitate: in elutriant, add the ethanol that massfraction is 70-80% while stirring, stir, sealing precipitation, collecting precipitation thing;
(5) dry: throw out to be put into baking oven and carry out drying, can heparin sodium be obtained.
Further, in described step (3), the consumption of 2709 alkaline enzymes and the mass ratio of waste material are 1:30, and described whipping temp is 45-60 DEG C.
Further, in described step (4), the volume of ethanol and the volume ratio of elutriant are 1.6-2:1, and described Sealing period is 24-26 hour.
Further, in described step (5), the temperature of baking oven is 80-90 DEG C, and the dry time is 5-7 hour.
Beneficial effect of the present invention is as follows: manufacture craft of the present invention is simple, and convenient operation, by the process to rim charge, effectively extracts heparin sodium, substantially increases the utilization to casing rim charge, avoids unnecessary waste.
Embodiment
By particular specific embodiment, embodiments of the present invention are described below, person skilled in the art scholar the content disclosed by this specification sheets can understand other advantages of the present invention and effect easily.
Embodiment 1
Animal waste extracts a preparation method for heparin sodium, through cleaning, extracts the preparation that glue, wash-out, precipitation and drying step complete heparin sodium; Concrete steps are as follows:
(1) clean: repeatedly clean under the rim charge of natural casing, gelatine, fish glue or hoof glue are put into clear water, wash number is 3 times;
(2) extract glue: the rim charge after above-mentioned cleaning, gelatine, fish glue or hoof glue are put into high temperature pot and dissolves, add resins exchange after dissolving and extract glue;
(3) wash-out: is added 2709 appropriate Sumizyme MPs in the water that exchange is separated, regulate PH to 8, and then add the salt solution that massfraction is 3%, stir at a certain temperature, churning time is 1 hour, carries out wash-out, extracts elutriant, the consumption of 2709 alkaline enzymes and the mass ratio of waste material are 1:30, and whipping temp is 45 DEG C;
(4) precipitate: in elutriant, add the ethanol that massfraction is 70% while stirring, stir, sealing precipitation, collecting precipitation thing, the volume of ethanol and the volume ratio of elutriant are 1.6:1, and Sealing period is 24 hours;
(5) dry: throw out is put into baking oven and carry out drying, can obtain heparin sodium, the temperature of baking oven is 80 DEG C, the dry time is 5 hours.
Embodiment 2
Animal waste extracts a preparation method for heparin sodium, through cleaning, extracts the preparation that glue, wash-out, precipitation and drying step complete heparin sodium; Concrete steps are as follows:
(1) clean: repeatedly clean under the rim charge of natural casing, gelatine, fish glue or hoof glue are put into clear water, wash number is 5 times;
(2) extract glue: the rim charge after above-mentioned cleaning, gelatine, fish glue or hoof glue are put into high temperature pot and dissolves, add resins exchange after dissolving and extract glue;
(3) wash-out: is added 2709 appropriate Sumizyme MPs in the water that exchange is separated, regulate PH to 9, and then add the salt solution that massfraction is 4%, stir at a certain temperature, churning time is 2 hours, carries out wash-out, extracts elutriant, the consumption of 2709 alkaline enzymes and the mass ratio of waste material are 1:30, and whipping temp is 60 DEG C;
(4) precipitate: in elutriant, add the ethanol that massfraction is 80% while stirring, stir, sealing precipitation, collecting precipitation thing, the volume of ethanol and the volume ratio of elutriant are 2:1, and Sealing period is 26 hours;
(5) dry: throw out is put into baking oven and carry out drying, can obtain heparin sodium, the temperature of baking oven is 90 DEG C, the dry time is 7 hours.
Embodiment 3
Animal waste extracts a preparation method for heparin sodium, through cleaning, extracts the preparation that glue, wash-out, precipitation and drying step complete heparin sodium; Concrete steps are as follows:
(1) clean: repeatedly clean under the rim charge of natural casing, gelatine, fish glue or hoof glue are put into clear water, wash number is 4 times;
(2) extract glue: the rim charge after above-mentioned cleaning, gelatine, fish glue or hoof glue are put into high temperature pot and dissolves, add resins exchange after dissolving and extract glue;
(3) wash-out: is added 2709 appropriate Sumizyme MPs in the water that exchange is separated, regulate PH to 8.5, and then add the salt solution that massfraction is 3.5%, stir at a certain temperature, churning time is 1.5 hours, carries out wash-out, extracts elutriant, the consumption of 2709 alkaline enzymes and the mass ratio of waste material are 1:30, and whipping temp is 53 DEG C;
(4) precipitate: in elutriant, add the ethanol that massfraction is 75% while stirring, stir, sealing precipitation, collecting precipitation thing, the volume of ethanol and the volume ratio of elutriant are 1.8:1, and Sealing period is 25 hours;
(5) dry: throw out is put into baking oven and carry out drying, can obtain heparin sodium, the temperature of baking oven is 85 DEG C, the dry time is 6 hours.
Manufacture craft of the present invention is simple, and convenient operation, by the process to rim charge, effectively extracts heparin sodium, substantially increases the utilization to casing rim charge, avoids unnecessary waste.
Above-described embodiment is preferred embodiment of the present invention; it is not the restriction to technical solution of the present invention; as long as without the technical scheme that creative work can realize on the basis of above-described embodiment, all should be considered as falling within the scope of the rights protection of patent of the present invention.
Claims (4)
1. animal waste extracts a preparation method for heparin sodium, it is characterized in that: through cleaning, extract the preparation that glue, wash-out, precipitation and drying step complete heparin sodium; Concrete steps are as follows:
(1) clean: repeatedly clean under the rim charge of natural casing, gelatine, fish glue or hoof glue are put into clear water, described wash number is 3-5 time;
(2) extract glue: the rim charge after above-mentioned cleaning, gelatine, fish glue or hoof glue are put into high temperature pot and dissolves, add resins exchange after dissolving and extract glue;
(3) wash-out: is added 2709 appropriate Sumizyme MPs in the water that exchange is separated, regulate PH to 8-9, and then add the salt solution that massfraction is 3-4%, stir at a certain temperature, described churning time is 1-2 hour, carries out wash-out, extracts elutriant;
(4) precipitate: in elutriant, add the ethanol that massfraction is 70-80% while stirring, stir, sealing precipitation, collecting precipitation thing;
(5) dry: throw out to be put into baking oven and carry out drying, can heparin sodium be obtained.
2. a kind of animal waste according to claim 1 extracts the preparation method of heparin sodium, it is characterized in that: in described step (3), the consumption of 2709 alkaline enzymes and the mass ratio of waste material are 1:30, and described whipping temp is 45-60 DEG C.
3. a kind of animal waste according to claim 1 extracts the preparation method of heparin sodium, it is characterized in that: in described step (4), the volume of ethanol and the volume ratio of elutriant are 1.6-2:1, and described Sealing period is 24-26 hour.
4. a kind of animal waste according to claim 1 extracts the preparation method of heparin sodium, it is characterized in that: in described step (5), the temperature of baking oven is 80-90 DEG C, and the dry time is 5-7 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510720913.5A CN105199014A (en) | 2015-10-30 | 2015-10-30 | Preparation method for extracting heparin sodium from animal waste materials |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510720913.5A CN105199014A (en) | 2015-10-30 | 2015-10-30 | Preparation method for extracting heparin sodium from animal waste materials |
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| CN105199014A true CN105199014A (en) | 2015-12-30 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831010A (en) * | 2010-05-21 | 2010-09-15 | 南阳鹏远肠衣有限公司 | Method for extracting heparin from animal casing waste |
| CN101891842A (en) * | 2010-07-16 | 2010-11-24 | 杭州龙扬生物科技有限公司 | Process for producing heparin sodium |
| CN104448049A (en) * | 2014-12-24 | 2015-03-25 | 青岛九龙生物医药有限公司 | Method for extracting heparin sodium from casing |
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2015
- 2015-10-30 CN CN201510720913.5A patent/CN105199014A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831010A (en) * | 2010-05-21 | 2010-09-15 | 南阳鹏远肠衣有限公司 | Method for extracting heparin from animal casing waste |
| CN101891842A (en) * | 2010-07-16 | 2010-11-24 | 杭州龙扬生物科技有限公司 | Process for producing heparin sodium |
| CN104448049A (en) * | 2014-12-24 | 2015-03-25 | 青岛九龙生物医药有限公司 | Method for extracting heparin sodium from casing |
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Application publication date: 20151230 |