CN103232556A - Enzymolysis technology of heparin sodium by using microwave process - Google Patents
Enzymolysis technology of heparin sodium by using microwave process Download PDFInfo
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- CN103232556A CN103232556A CN 201310143758 CN201310143758A CN103232556A CN 103232556 A CN103232556 A CN 103232556A CN 201310143758 CN201310143758 CN 201310143758 CN 201310143758 A CN201310143758 A CN 201310143758A CN 103232556 A CN103232556 A CN 103232556A
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- heparin
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Abstract
The invention discloses an enzymolysis technology of heparin sodium by using a microwave process. The enzymolysis technology is characterized by comprising the following steps of: washing a mucous membrane of a small intestine of a fresh pig with clean water; grinding into meat paste by using a meat grinder; adding water into the meat pastes to ensure the mass ratio of water to the mucous membrane of the small intestine of the pig is 8-10:1; adding 0.2-0.3 wt% of trypsin; adding alkali to regulate a pH value to 8.5-9; stirring and reacting for 30-60 minutes, then adding 3-5 percent of sodium chloride solutions; extracting in a microwave stove for 12-15 minutes at microwave power of 300-400W; and after the reaction is finished, filtering to obtain enzymatic hydrolysate. The enzymolysis technology has the advantages that heparin in the small intestine of the pig is extracted by combining microwave method, enzymolysis method and salt solution method together, so that rich raw materials are supplied in a subsequent extraction process of heparin sodium; and the method is simple in process, short in operation time and high in heparin extraction efficiency.
Description
Technical field
The present invention relates to the enzymolysis process that a kind of microwave method is used for heparin sodium.
Background technology
Heparin sodium is a kind of mucopolysaccharide sulfuric ester thunder anticoagulant, is mainly used in treating the acute myocardial infarction plug, can alleviate the effect of unstable angina pectoris, antithrombotic formation and reducing blood-fat, decreasing cholesterol.People had found heparin first in liver in 1916, found that then content is abundanter in pig intestinal mucosa.So people begin to extract heparin sodium from pig intestinal mucosa.And the extraction effect of heparin directly influences extraction yield and the output of final heparin sodium, and method commonly used has enzymolysis process or salt solution, though energy success heparin is extracted can cause product losses, and extraction time is long, influences the production efficiency of heparin sodium.
Summary of the invention
The purpose of this invention is to provide the enzymolysis process that a kind of microwave method is used for heparin sodium, have weak point consuming time, advantage that extraction efficiency is high.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is: a kind of microwave method is used for the enzymolysis process of heparin sodium, it is characterized in that: get the fresh pig mucous membrane of small intestine and clean with clear water, be twisted into the meat gruel shape by mincer, to wherein adding water, the mass ratio of water and pig intestinal mucosa is 8-10:1, adds the trypsinase of 0.2-0.3wt% again, adds alkali and regulates the pH value between the 8.5-9, behind the stirring reaction 30-60min, add the sodium chloride solution of 3-5% again, place microwave oven, microwave power is 300-400W, extraction time is 12-15min, after the end, filter, obtain enzymolysis solution.
The invention has the advantages that: the present invention adopts microwave method and enzymolysis process, salt solution to combine, heparin in the chitterlings is extracted, for the leaching process of follow-up heparin sodium provides abundant raw material, this procedure is simple, the operating time short, heparin extraction efficiency height.
Embodiment
In order to make the public can fully understand technical spirit of the present invention and beneficial effect; the applicant will describe in detail the specific embodiment of the present invention below by reference to the accompanying drawings; but the applicant is not restriction to technical scheme to the description of embodiment, anyly makes form but not the variation of essence all should be considered as protection scope of the present invention according to the present invention's design.
Embodiment 1
A kind of microwave method is used for the enzymolysis process of heparin sodium, and step is: get the fresh pig mucous membrane of small intestine and clean with clear water, be twisted into the meat gruel shape by mincer, to wherein adding water, the mass ratio of water and pig intestinal mucosa is 8:1, adds the trypsinase of 0.2wt% again, add alkali and regulate the pH value between the 8.5-9, behind the stirring reaction 30-60min, add 3% sodium chloride solution again, place microwave oven, microwave power is 300W, and extraction time is 12min, after the end, filter, obtain enzymolysis solution.
The heparin amount of Ti Quing is according to the method described above: the heparin of 100,000,000 activity units needs 1104 pairs of chitterlings.
Embodiment 1
A kind of microwave method is used for the enzymolysis process of heparin sodium, and step is: get the fresh pig mucous membrane of small intestine and clean with clear water, be twisted into the meat gruel shape by mincer, to wherein adding water, the mass ratio of water and pig intestinal mucosa is 10:1, adds the trypsinase of 0.3wt% again, add alkali and regulate the pH value between the 8.5-9, behind the stirring reaction 30-60min, add 5% sodium chloride solution again, place microwave oven, microwave power is 400W, and extraction time is 15min, after the end, filter, obtain enzymolysis solution.
The heparin amount of Ti Quing is according to the method described above: the heparin of 100,000,000 activity units needs 1099 pairs of chitterlings.
Claims (1)
1. a microwave method is used for the enzymolysis process of heparin sodium, it is characterized in that: get the fresh pig mucous membrane of small intestine and clean with clear water, be twisted into the meat gruel shape by mincer, to wherein adding water, the mass ratio of water and pig intestinal mucosa is 8-10:1, the trypsinase that adds 0.2-0.3wt% again, add alkali and regulate the pH value between the 8.5-9, behind the stirring reaction 30-60min, add the sodium chloride solution of 3-5% again, place microwave oven, microwave power is 300-400W, and extraction time is 12-15min, after the end, filter, obtain enzymolysis solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310143758 CN103232556A (en) | 2013-04-24 | 2013-04-24 | Enzymolysis technology of heparin sodium by using microwave process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310143758 CN103232556A (en) | 2013-04-24 | 2013-04-24 | Enzymolysis technology of heparin sodium by using microwave process |
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| CN103232556A true CN103232556A (en) | 2013-08-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN 201310143758 Pending CN103232556A (en) | 2013-04-24 | 2013-04-24 | Enzymolysis technology of heparin sodium by using microwave process |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103694373A (en) * | 2013-11-23 | 2014-04-02 | 青岛九龙生物医药有限公司 | Method for removing DS impurity in refined heparin sodium |
| CN103804506A (en) * | 2014-02-11 | 2014-05-21 | 华中科技大学 | Method for extracting heparin and dermatan sulfate from small intestine lixivium |
| CN103804520A (en) * | 2013-11-22 | 2014-05-21 | 青岛九龙生物医药有限公司 | Method for improving test index of heparin sodium competitive product 260nm absorbancy |
| WO2020133063A1 (en) * | 2018-12-27 | 2020-07-02 | 青岛惠博士生物科技有限公司 | Application of small intestinal mucosa active substance in preparation of food, health products, special clinical diets or medicines, products of small intestinal mucosa active substance, and method |
-
2013
- 2013-04-24 CN CN 201310143758 patent/CN103232556A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103804520A (en) * | 2013-11-22 | 2014-05-21 | 青岛九龙生物医药有限公司 | Method for improving test index of heparin sodium competitive product 260nm absorbancy |
| CN103694373A (en) * | 2013-11-23 | 2014-04-02 | 青岛九龙生物医药有限公司 | Method for removing DS impurity in refined heparin sodium |
| CN103804506A (en) * | 2014-02-11 | 2014-05-21 | 华中科技大学 | Method for extracting heparin and dermatan sulfate from small intestine lixivium |
| WO2020133063A1 (en) * | 2018-12-27 | 2020-07-02 | 青岛惠博士生物科技有限公司 | Application of small intestinal mucosa active substance in preparation of food, health products, special clinical diets or medicines, products of small intestinal mucosa active substance, and method |
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Application publication date: 20130807 |