CN105191938B - One grows tobacco anti-cryogen and its preparation and application - Google Patents
One grows tobacco anti-cryogen and its preparation and application Download PDFInfo
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Abstract
The invention discloses a kind of anti-cryogen and its preparation and application for improving tobacco seedling winter resistance.The anti-cryogen contains following main component：1.15~2.30g/L of glycine betaine, 5.0~10.0mg/L of salicylic acid, abscisic acid 3.0mg~5.0mg/L, 5 600~1000mg/L of amino-laevulic acid.In " also seedling stage ", " stretching the root phase " foliage-spray before tobacco seedlings go out garden or after tobacco seedlings field-transplanting, concentration after 3~5 times of dilutions of stoste to spray, every mu of Application is 25~30L.The present invention is the cold-resistant foliage spraying agent prepared by physiologically active Auto-regulator, defensive ability/resistance ability of the tobacco shoot (seedling) to Chilling stress can be effectively improved, the fertility of tobacco seedling caused by " cold spell in later spring " that southern tobacco main producing region takes place frequently can be preferably tackled to be obstructed the chilling damage of (or even " early blossoming "), Flue-cured tobacco Quality is also had no adverse effects, is had a good application prospect on tobacco leaf production.
Description
Technical field
Chilling stress resisted the invention belongs to tobacco, suppress " early blossoming " generation technique field, and in particular to one kind is for carrying
The anti-cryogen and its preparation and application of tobacco seedling winter resistance high.
Background technology
Tobacco is to originate in the semi-tropical warm crop of happiness (its growth optimum temperature is 25~28 DEG C, and 10~13 DEG C stop life
Long, 1~2 DEG C of low temperature can make death of seedling), environment temperature (especially low temperature) has aobvious to the fertility of tobacco, yield and quality
The influence of work.China's cigarette district is widely distributed, and various regions temperature conditionss differ greatly, and such as Main Tobacco-growing Regions In South " cold spell in later spring " takes place frequently, and causes " also
Seedling stage, stretch the root phase " tobacco plant fertility is bad and induces the generation of " early blossoming ", has a strong impact on yield of tobacco and quality, and also one
The straight development that govern sound tobacco production.At present, the Comprehensive Traits ratio of the main breeds such as K326, the cloud and mist 87 promoted in production
It is more excellent, but its patience to low temperature environment is poor.
This practical problem is endangered for " cold spell in later spring " taken place frequently in puzzlement tobacco leaf production, low temperature is bred as by genetic improvement
Germ plasm resource needed for patience new product of tobacco lacks, and breeding cycle is long and needs substantial amounts of human and material resources and financial resources, category strategy
Property behave；And pass through agronomic measures and such as spray the Heat pretreatment that tobacco Heat pretreatment conditioning agent improves tobacco plant, then can yet be regarded as
The effective measures and method of early spring " cold spell in later spring " harm in reply tobacco leaf production.
Therefore, develop and develop suit improve tobacco Heat pretreatment technical essential tobacco Heat pretreatment conditioning agent become
Problem demanding prompt solution in tobacco leaf production, it is significant to guarantee and the safety in production of stabilization tobacco leaf.
The content of the invention
Blank it is an object of the invention to make up tobacco chilling damage counter-measure technical field, there is provided one kind can be improved
The cold-resistant composite foliar spray of tobacco seedling Heat pretreatment, and with method of preparation and use is simple, effect substantially, cost
Cheap the advantages of.
First purpose of the invention is to provide the formula (constituent) of above-mentioned anti-cryogen.
One grows tobacco anti-cryogen, is the solution comprising following density component：1.15~2.30g/L of glycine betaine, salicylic acid 5.0
~10.0mg/L, abscisic acid 3.0mg~5.0mg/L, 600~1000mg/L of 5-ALA.
The concentration comprising glycine betaine is preferably 1.15mg/L in the anti-cryogen；Salicylic concentration is preferably 5.0mg/L；
The concentration of abscisic acid is preferably 3.0mg/L；The concentration of 5-ALA is preferably 0.6g/L.
Described anti-cryogen, also contains glycerine, Vc, calcium salt and trace element.
Calcium salt in the anti-cryogen is the one kind in nitrate or calcium chloride, and the concentration of calcium ion is 600~1000mg/
L；The concentration of the Vc in the anti-cryogen be 500~1000mg/L, glycerine concentration be 15~20ml/L；In every liter of anti-cryogen also
Comprising 5~10mg/L of trace elements iron, 5~10/L of boron mg, 0.5~1.0mg/L of manganese, 10~25mg/L of zinc, molybdenum 0.05~
0.10mg/L)。
Comprising in 0.5ml plant leaf blades surface active agent tween 20, Tween 80, triton x-100 in every liter of anti-cryogen
One or more.
It is a further object of the present invention to provide the preparation method of above-mentioned anti-cryogen.
The preparation method of anti-cryogen of the invention, comprises the following steps successively：
(1) salicylic acid is dissolved in appropriate absolute ethyl alcohol or 95% ethanol, obtains solution I；
(2) component glycine betaine, 5-ALA, Vc, glycerine, calcium salt and trace element are added to the water, are constantly stirred
Mix to it and be completely dissolved acquisition solution II；
(3) abscisic acid is dissolved in appropriate absolute ethyl alcohol or 95% ethanol, obtains solution III；
(4) solution I, III are added separately in solution II and add surfactant, being stirred continuously is sufficiently mixed it
Uniformly, anti-cryogen stoste of the invention is obtained, is diluted again when using.
It is a still further object of the present invention to provide the application method of above-mentioned anti-cryogen.
The anti-cryogen is in tobacco seedlings foliage-spray.Specifically " seedling is gone back behind before tobacco seedlings go out garden 5~7 or tobacco seedlings field-transplanting
Phase ", the rain or shine day dusk foliage-spray of " stretching the root phase " breeding time.To be sprayed after 3~5 times of dilutions of anti-cryogen when using, every mu of spray
Amount of application is 25~30L.It is required that being uniformly sprayed onto on tobacco plant blade tow sides, whole blade is moistened completely with solution
It is defined.The planting density of tobacco leaf production, usually 1100~1300 or 1300~1500 plants/acre (various regions tobacco office has unified
Regulation).
The anti-cryogen of tobacco of the invention is directed to after tobacco seedlings transplant, and early spring microthermal climate (" cold spell in later spring ") is in " going back seedling
Phase ", the designed research and development of harmful effect of " stretching the root phase " tobacco seedling fertility.
On the one hand, the reason for " cold spell in later spring " weather causes seedling growth and development bad mainly includes it and meets with low temperature stress
When：(1) membrane structure " impaired " of tobacco seedling, permeability increase, intracellular solable matter and electrolyte can passively to
The outer seepage of film；(2) tobacco seedling itself is weak to the resistivity of low temperature, and its metabolism " disorder " will be caused when meeting with low temperature stress,
The compatible solute such as soluble sugar, proline and glycine betaine content reduction, Heat pretreatment weakens；(3) tobacco seedling active oxygen is clear
Except system capability declines, the large biological molecule such as active oxygen direct oxidation damaging cells internal protein, enzyme, nucleic acid of enrichment；(4) cigarette
The enzyme activity reduction of careless seedling leaves photosynthetical system, chlorophyll are oxidized and decompose, and cause tobacco seedlings plant photosynthetic capacity to decline.The opposing party
Face, the anti-cryogen that the present invention is used includes the life of the plants such as glycine betaine, salicylic acid, abscisic acid, 5-ALA, Vc, glycerine
Reason active material, the cytoplasma membrane to meeting with Chilling stress stress tobacco plant has good protective effect, can increase tobacco seedlings
The compatible solute content of reply Chilling stress stress, mitigates the decline of anti-oxidant enzyme activity, when reduction experience Chilling stress is coerced
The accumulation of active oxygen, maintains the physiologic effects such as tobacco plant photosynthetic activity；Additionally, as the abscisic acid controllable of " resistance hormone "
Strengthen the stress resistance of plant.
Therefore, the present invention is started with from above-mentioned many-sided consideration, modulation glycine betaine, salicylic acid, abscisic acid, 5- glycyls
The compound formulation of the physiologically active Auto-regulator such as propionic acid, Vc, glycerine, processes to improve the low of tobacco plant through tobacco seedlings foliage-spray
Warm adverse circumstance resistivity, so as to promote the fertility status for meeting with " cold spell in later spring " Chilling stress stress tobacco seedlings to tend to normal and mitigate
Suppress the generation of " early blossoming ", had a good application prospect in leaf tobacco production.
Brief description of the drawings
Fig. 1 be tobacco plant Chilling stress response ratio compared with；
A：Here adjoining tree withers, B：The anti-cryogen treatment plant of the present invention, C/D/E：Adjoining tree blade face chilling injury symptom；
Fig. 2 is to recover with experience Chilling stress stress tobacco plant normal temperature not processed by the invention by present invention treatment
Growth conditions compare；
Fig. 3 is to budding situation by present invention treatment and experience Chilling stress stress tobacco plant not processed by the invention
Compare；
Fig. 4 is to be bloomed situation by present invention treatment and experience Chilling stress stress tobacco plant not processed by the invention
Compare；
Fig. 5 is anti-cryogen productivity crop field pilot scale of the invention, A：Field experiment, B：Flue-cured tobacco analyzes sample.
Specific embodiment
By the present invention in that modulating various cold-resistant foliage-sprays with the physiologically active Auto-regulator of variety classes and proportioning
Agent, and its effect is compared by experimental verification, filter out the cold-resistant foliage spraying agent of optimal component and proportioning.By following tool
The implementation method of body further illustrates technical scheme, is not intended to limit the present invention.
Embodiment 1
Present invention experiment is carried out in the Central-South tobacco experiment station of Agricultural University Of Hunan's Chinese tobacco.Produced with Tobacco In Hunan
Main breed K326, cloud and mist 87 and Hunan cigarette 3 as tested variety, using tobacco seedling dedicated substrate, using floating seedlings method
Nursery；1~2 leaf period thinning, 3~4 leaf period transplanting are potted plant after emerging, and rich water pipe is carried out according to tobacco seedling technology
Reason, is extremely bred as being 6~8 leaf period tobacco seedlings for experiment.
First, the comparing of physiologically active of plant Auto-regulator foliage-spray treatment effect
It is a large amount of to consult domestic foreign minister according to physiologically active of plant Auto-regulator and the correlation of tobacco seedling stress resistance
Documents and materials are closed, from spermidine (Spd), glycine betaine (BH), proline (Pro), polyethylene glycol (PEG), 5- glycyls third
14 kinds of physiologically active of plant materials in close relations with plant stress resistance such as sour (5-ALA), carry out tobacco seedling of the present invention low
The screening of warm patience conditioning agent component, and (be shown in Table by the suitable concentration that pilot study determines each component tobacco foliar treatment
1)。
1 14 kinds of physiologically active Auto-regulators of table and its concentration
1st, influence of the physiologically active of plant Auto-regulator treatment to tobacco seedling stress response
By (25 DEG C of 5000lx, light after 6~8 Potted tobacco seedling immigration phjytotron normal temperature domestication 3d of leaf age
According to 12h), 14 kinds of physiologically active of plant Auto-regulators process tobacco seedling (spray clear water with respective suitable concentration foliage-spray
It is control)；After spraying treatment 1 day (24h), start low temperature and poor light and process 10 days (8 DEG C of 1000lx, illumination 12h) according to adverse circumstance.
1.1 tobacco seedlings Chilling stress are responded
Low temperature and poor light observes tobacco seedlings Chilling stress responsive status, record according to adverse circumstance treatment the 0th, 0.5,1,2,3,7d noons
Evaluate the wilting degree of the whole strain tobacco seedlings of each treatment and blade, damage to plants caused by sudden drop in temperature symptom etc..Result shows：During Chilling stress treatment, control
Most of tobacco seedling there is different degrees of wilting, there is waterlogging spot or leaf margin rearwardly in the blade of part control seedling
Curling；The foliar treatment of most of physiologically active of plant Auto-regulator can significantly mitigate seedling wither here degree, wherein spermidine
(Spd), the foliar treatment such as glycine betaine (BH), 5-ALA (5-ALA), proline (Pro), salicylic acid (SA) and ABA
Most of seedling there is not chilling injury symptom.
1.2 recover tobacco plant upgrowth situation and situation of buddingging after normal temperature
(1) after 10d low-temperature treatments terminate, potted plant tobacco seedlings are placed in normal temperature normal management, observation tobacco seedling growth and shape of buddingging
Condition.Observation finds：In normal temperature initial stages of restoration, the tobacco seedlings normal growth that most of physiologically active Auto-regulator is processed compared with the control
Recover very fast, all tobacco seedlings of 3 kinds restore normal growth after 15d；Later stage (25d) observation display, part physiologically active is adjusted
Section material treatment tobacco seedling growth is more vigorous, growth potential is better than control.
(2) after normal temperature restoration ecosystem 30,45,60d tobacco seedlings condition survey of buddingging.Observation finds：Same kind, different lifes
There are obvious difference, glycine betaine, proline, 5- glycyls tobacco plant time of buddingging that reason Active Regulation material sprays treatment
The time of buddingging of propionic acid, salicylic acid and ABA treatment plant relatively compares substantially " postponement ".
2nd, Chilling stress treatment tobacco seedlings blade electrical conductivity detection
Relative conductivity is an important physical signs for reflecting biofilm system situation.When plant meets with environment stress
When, cell membrane is destroyed, permeability increase, and the extravasation of electrolyte inside cell will cause plant tissue leaching liquor electrical conductivity to increase,
That is membrane permeability increase degree is negatively correlated with stress resistance of plant.
Low temperature and poor light according to treatment during the 0th, 2,4,6,8d noons, to 14 kinds of physiologically active of plant Auto-regulators treatment tobacco seedlings
The relative conductivity of the 4th or 5 blades detected, further to verify that it influences on tobacco seedlings Chilling stress resistance.Detection
Result shows：Compared with the control, most of physiologically active of plant Auto-regulator treatment can mitigate caused by Chilling stress stress
The increase of blade relative conductivity, with glycine betaine, 5-ALA, proline, salicylic acid and ABA, this 5 kinds of plants give birth to especially
Manage the mitigation effect of Active Regulation material substantially, that is, enhance the Chilling stress resistance of tobacco seedlings.
Overall merit is participated in the experiment the Chilling stress response of 3 kinds, 14 kinds of physiologically active of plant Auto-regulator foliar treatment seedling
Situation (the table of performance (blade wilting degree, damage to plants caused by sudden drop in temperature symptom, blade electrical conductivity), normal temperature restoration ecosystem and the aspect of performance three of buddingging
2), and consider its physicochemical property, in this 14 kinds of physiologically active of plant Auto-regulators treatment, glycine betaine (BH), proline (Pro),
Enhancing of the treatment of salicylic acid (SA), 5-ALA (5-ALA) and abscisic acid (ABA) to tobacco seedling Heat pretreatment is made
With obvious, it is good that its treatment seedling shows stronger low-temperature resistance, normal temperature restoration ecosystem.Accordingly, by this five kinds of physiology
The main candidate composition that Active Regulation material is considered as the anti-cryogen of tobacco carries out further research.
Overall merit of the 2 14 kinds of physiologically active Auto-regulators of table on the response influence of tobacco bred K326 seedling Chilling stress
Note：The evaluation of tobacco seedling low temperature stress reaction：
2nd, the screening of physiologically active of plant Auto-regulator compound prescription
1st, basic situation is tested：The candidate confirmed according to early stage single plant physiologically active Auto-regulator preliminary screening into
Divide and its concentration, design physiologically active Auto-regulator compound prescription combines 8 (table 3), foliage-spray starts low temperature after processing 1
The treatment of low-light adverse circumstance.Tested variety is K326 and cloud 87, and the incubation of experiment material, phjytotron domestication, low temperature and poor light shine
Adverse circumstance treatment conditions, observation item and evaluation criterion are identical with previous experiments.Low temperature and poor light according to treatment during, the 0th, 0.5,1,2,
3rd, 5 at, 7d noons, observe, record the Chilling stress response (wilting degree, damage to plants caused by sudden drop in temperature symptom) of each compound prescription treatment tobacco seedlings, the 0th,
1st, 3, determine each treatment tobacco seedlings leaf photosynthesis activity in the 5d noons, the 0th, 2,4,6, gather in the 8d noons the fresh sample of each treatment tobacco leaf,
After liquid nitrogen frozen grinds, -80 DEG C of low temperature refrigerators are preserved for physico-chemical analysis, and experimental result is shown in Table 4,5.
3 physiologically active Auto-regulator of table, 8 kinds of compound prescription combinations and its concentration
Note：Eight combinations are respectively glycine betaine (B), salicylic acid (S), ABA (Ab), 5-ALA (A), proline (P) in table
Various combination is formulated.
2nd, the result of compound prescription conditioning agent and analysis (table 4)
2.1 Chilling stress are responded
It is low during overall merit tested variety K326, cloud and mist 87, the treatment tobacco seedlings Chilling stress treatment of different composite formula
Temperature response (the damaging to plants caused by sudden drop in temperature symptom, wilting degree etc. of tobacco seedlings), combines the low temperature for 2., 3., 6., 8. processing tobacco seedlings in 8 compound prescriptions
Stress response is substantially better than control.
2.2 photochemical vitalities
The photosynthetic activity of two tested variety tobacco seedlings has declined (data summary) during Chilling stress treatment, but compound prescription
Treatment can mitigate the decline degree of tobacco seedlings photosynthetic activity, tobacco seedlings its photosynthetic activity decline that wherein 2., 3., 6. compound prescription is processed
Amplitude is smaller, other compound prescriptions treatment tobacco seedlings decline by a big margin or suitable with control.
2.3 normal temperature growths recover
10d low-temperature treatments terminate, recover normal temperature growth condition.After normal temperature restoration ecosystem 15d, the cigarette of all treatment of two kinds
Miao Jun restore normal growth, and the tobacco seedling growth that wherein 2., 3., 6. compound prescription is processed is more vigorous (being substantially better than control).
8 kinds of physiologically active of plant Auto-regulator compound prescription foliage-spray treatment of generalized analysis are to the two kinds children that participates in the experiment
Upgrowth situation during the influence and normal temperature recovery of the response of seedling Chilling stress, compound prescription general performance 2., 3., 6. is more excellent,
Further checking can be made as candidate's compound prescription, to its physiologic effect to screen.
Preliminary assessment of the physiologically active Auto-regulator compound prescription of table 4 on the response influence of tested variety seedling Chilling stress
In addition, can be seen that the combined effect containing proline (Pro) from the physiologic effect of above-mentioned 8 compound prescriptions owing
It is good；Therefore, " rejecting " proline in five kinds of Candidate components, by glycine betaine (BH), salicylic acid (SA), abscisic acid (ABA) and 5- ammonia
Four kinds of components of base levulic acid (5-ALA) are used for further screening compound prescription as preferred component.
3rd, influence of four kinds of preferred plant physiologically active Auto-regulators treatment to tobacco stress physiology biochemical indicator
Its elementary tactics is active accommodation own metabolism when plant meets with environment stress, strengthens single-minded Resistance Physiology generation
Thank, accumulate compatible solute, such as increase soluble sugar, proline endogenous cellular Protective substances content and improve anti-oxidant
The activity of enzyme in system；Meanwhile, by intracellular compatible solute Scavenger of ROS, mitigate oxidation directly injury or performance molecule
The effect of companion carrys out the 26S Proteasome Structure and Function of Protein requirement, protects the plants from injury.Therefore, the related physiology life of stress resistance
Change the important evidence that index is the stress resistance for evaluating plant.
Further screening is made for the preferred ingredient for resisting cryogen to determine, to 4 kinds of preferred component glycine betaines (BH), bigcatkin willows
Sour (SA), abscisic acid (ABA) and 5-ALA (5-ALA) foliar treatment K326 tobacco seedlings (incubation of material is the same),
Low temperature and poor light according to treatment the 0th, 2,4,6,8d noons sample carries out physiological and biochemical analysis detection；Testing index：Chlorophyll, the third two
Aldehyde, proline, soluble protein, soluble sugar, ascorbic acid, hydrogen peroxide, content of oxygen free radical.
Overall merit of the 5 four kinds of physiologically active Auto-regulators of table to K326 seedling stress physiology Index Influences
Note：Change compared with the control：Dramatically increase ++ increase+quite ± reduce-substantially reduce --
Overall merit：Better than control √ be inferior to control ×
Systematic analysis technique glycine betaine, salicylic acid, abscisic acid and 5-ALA 4 kinds of physiologically active Auto-regulators
Foliar treatment shows tobacco seedling stress physiology biochemical (summary of table 5, data)：This 4 kinds of physiologically active of plant regulations
The treatment of material, can make the content of the compatible solute such as proline, soluble protein, soluble sugar in tobacco seedling increases,
The accumulation of MDA and active oxy group is reduced, the oxidative damage of cellulose, chlorophyll is alleviated, it is inverse to tobacco seedling
The enhancing of border resistance has facilitation (consistent with the experimental result that early stage tobacco seedling Chilling stress is responded)；This experimental result is entered
One step confirms that this 4 kinds of physiologically active of plant Auto-regulators can be used as the preferred ingredient of the anti-cryogen compound prescription of tobacco.
Embodiment 2
Four kinds of preferred ingredients that embodiment 1 is finally obtained are obtained into new formula with reference to other auxiliary elements.
(amount) is claimed to take glycine betaine 1.15g, salicylic acid 5.0mg, 5-ALA 0.6g, abscisic acid 3mg, glycerine
20ml, polysorbas20 (Tween 20) 0.5ml, Vc 0.50g, calcium chloride 0.6g and trace elements iron (Fe) 5mg, boron (B) 5mg, manganese
(Mn) 0.5mg, zinc (Zn) 10mg, molybdenum (Mo) 0.05mg, for the preparation of tobacco seedling cold-resistant foliage spraying agent.
Component salicylic acid is first dissolved in 10ml absolute ethyl alcohols, solution I is obtained；
By in component glycine betaine, 5-ALA, glycerine, Vc, calcium chloride and trace element addition 1L water, constantly stir
Mix until fully dissolving obtains solution II；
Component abscisic acid is dissolved in 10ml absolute ethyl alcohols, solution III is obtained；
Finally solution I, III are added separately in solution II and polysorbas20 is added, being stirred continuously makes it be sufficiently mixed
It is even, cold-resistant foliage spraying agent of the invention is obtained.
Embodiment 3
Weigh glycine betaine 1.85g, salicylic acid 10mg, 5-ALA 1g, abscisic acid 4mg, remaining component, content with
And preparation steps are with embodiment 2.
Embodiment 4
Weigh glycine betaine 2.25g, salicylic acid 8mg, 5-ALA 0.8g, abscisic acid 5mg, remaining component, content
And preparation steps are with embodiment 2.
In order to further prove the effect of anti-cryogen formula of the invention, proceed the verification experimental verification of embodiment 5~8.
Embodiment 5：Anti- cryogen prepared by the embodiment of the present invention 2 processes the influence to tobacco seedling Resistant physiological index
Tested in 4~July in 2013,4~July in 2014, in the Central-South large-scale people in tobacco experiment station of Agricultural University Of Hunan
Work climatic chamber is carried out.Experimental cultivar is K326 and cloud 87, and experiment material is bred as with embodiment 1.From growing, vigorous, growing way is basic
Consistent seedling moves into phjytotron, and normal temperature tames 3d (22~23 DEG C, 5000lx, illumination 12h)；Anti- cryogen foliage-spray
Start Chilling stress treatment (8 DEG C, 1000lx, illumination 12h) after 24h.
Setup Experiments：Anti- cryogen group (treatment group) and fresh water spraying group (control group) prepared by embodiment 2, every group sprays place
Manage 10 plants (drenched with blade upper and lower surface is degree), be repeated 3 times；After spraying treatment 24h, start Chilling stress and process 7 days.Low temperature
Adverse circumstance treatment terminates rear phjytotron and recovers normal temperature condition, and from phjytotron be moved back to treatment tobacco seedlings after 3 days by normal temperature condition
To outdoor natural growing environment cultivation management, the fertility condition of period observation investigation tobacco plant.
Determine the electrical conductivity of mature leaf within the 0th, 1,3,5 days during Chilling stress treatment, and gather middle part climax leaves sample
Product are analyzed for physiological and biochemical index.Predominantly detect index have chlorophyll content, soluble sugar content, free proline content,
SOD enzyme activity, mda content (table 6).
As can be seen from Table 6：Under the conditions of same experience Chilling stress, the Resistance Physiology of anti-cryogen treatment group tobacco seedling
Biochemical indicator is substantially better than control group (variation tendency of two kinds of participating in the experiment is similar)；Illustrate that anti-cryogen processes the single-minded life of plant
Reason metabolism enhancing, the amount that improve compatible solute, while active oxygen elimination ability is also strengthened, it is to improve tobacco seedling
Heat pretreatment provides physiological and biochemical basis.
Influence of the anti-cryogen of table 6 treatment to experience Chilling stress tobacco plant physiological and biochemical index
Embodiment 6：The influence of anti-cryogen treatment prepared by embodiment 2 to experience Chilling stress tobacco plant photosynthetic activity
Tested in 4~July in 2013,4~July in 2014, in the Central-South large-scale people in tobacco experiment station of Agricultural University Of Hunan
Work climatic chamber is carried out, and experiment material and its incubation, Setup Experiments, Chilling stress treatment per 5 plants of experimental group, are repeated with embodiment 5
3 times.The 0th, 1,3,5 days during Chilling stress treatment are afternoon, and Chilling stress process phase is detected using the photosynthetic analyzers of Li-6400
Between tobacco plant mature leaf photosynthetic activity, predominantly detect index for Net Photosynthetic Rate (Pn), stomatal conductance (Gs), intercellular
CO2Concentration (Ci).
Influence of the anti-cryogen of table 7 treatment to experience Chilling stress tobacco plant photosynthetic activity
Temperature is one of principal element of influence photosynthesis of plant, and bad environment temperature (high temperature or low temperature) will be to it
Photochemical vitality produces obvious inhibitory action, causes its photosynthetic capacity to reduce and induces the photosynthetic low temperature light of plant
Suppress.The Net Photosynthetic Rate of plant leaf blade is one of leading indicator of Photosynthetic ability of mesophyll size；Stomatal conductance represents gas
The stretching degree in hole, has influence on blade O2、CO2With exchanging for steam and external environment, so as to influence photosynthesis；When some " bars
The timing of part ", the reduction of Photosynthetic ability of mesophyll will cause intercellular CO2Concentration increases.
Photosynthetic activity testing result shows (table 7)：Met with the adverse environmental factor that low temperature and poor light shines same, anti-cryogen treatment
Group plant has photosynthetic activity higher, main photosynthetic activity index net photosynthesis activity, stomatal conductance and intercellular CO2The change of concentration
Change is superior to control group (variation tendency of two kinds of participating in the experiment is similar to).May infer that：When meeting with Chilling stress, resist compared with the control
The photosynthetic capacity of cryogen treatment tobacco plant can maintain higher level, and the harmful effect that tobacco gives birth to is carried to mitigate cold spell in later spring
For " material " basis, it is beneficial to " also seedling stage ", " stretching the root phase " tobacco plant and grows.
Embodiment 7：Anti- cryogen prepared by embodiment 2 is processed to the response of experience Chilling stress tobacco plant Chilling stress, often
Warm growth recovery, budding, the influence of flowering time
Test in 4~July in 4~7,2015 in 2014, in the Central-South tobacco experiment station of Agricultural University Of Hunan's Chinese tobacco
Carry out.Incubation, experimental design and the adverse circumstance treatment of experiment material are same as Example 5.Tobacco plant is observed during adverse circumstance treatment
Chilling stress response performance (the wilting degree of whole strain and blade, damage to plants caused by sudden drop in temperature symptom etc.)；Room is moved to after Chilling stress Stress treatment
Outer normal temperature condition cultivation management, observes the fertility condition of tobacco plant：Growth recovery state (the growth recovery speed at normal temperature initial stage
Rate), florescence number of days different of buddingging, effective blade number.
The treatment of the anti-cryogen of table 8 is to meeting with the influence that Chilling stress tobacco plant is given birth to
Observation result during being processed from adverse circumstance with normal temperature restoration ecosystem can be seen that (table 8)：It is weak in same experience low temperature
Under conditions of illumination, the Chilling stress response of anti-cryogen treatment group plant is substantially better than control (Fig. 1), and normal temperature condition recovers just
Phase, the growth (growth recovery speed) for the treatment of group plant is also significantly better than control group (Fig. 2)；Normal temperature growth later stage, control group is planted
Strain is buddingged, blooms, and one week or so (Fig. 3,4) of postponement of blooming of buddingging for the treatment of group plant.Test result indicate that：Anti- cryogen treatment
The Chilling stress resistance that tobacco plant can be strengthened can mitigate or suppress low temperature stress institute, " damage " that reduces Chilling stress
Caused tobacco buddings early blossoming.Additionally, treatment group tobacco plant effectively the number of sheets compared with control group increase about 2~3.
Embodiment 8：Influence of the anti-cryogen treatment to tobacco flue-cured tobacco main components index obtained in embodiment 2
Test in 3~August in 2015, in Changsha, cigarette district, Chaling cigarette district and Yongzhou cigarette district have carried out the pilot scale of productivity crop field,
Experimental cultivar is respectively K326, cloud 87 and Hunan cigarette 5.Experimental design is divided into anti-cryogen treatment region and fresh water spraying check plot, often
Individual experimental group is repeated 3 times, is 20 × 3m per experimental group area2.Tobacco seedlings field-transplanting sprays anti-cryogen after 15 days, field thereafter
Cultivation management with commonly produce identical (Fig. 5 A)；The tobacco leaf picking phase wins middle part Mature Tobacco Leaves and is made flue-cured tobacco sample (Fig. 5 B), point
Analyse main Flue-cured tobacco Quality component target：Total reducing sugar, reduced sugar, nicotine, total nitrogen, K+、Cl-, content of starch.
Influence of the cold-resistant foliage spraying agent of table 9 treatment to tobacco flue-cured tobacco main components index content (%)
Be can be seen that (table 9) from the analysis result of three middle pilot flue-cured tobacco sample main components：Anti- cryogen treatment region
The main quality index content of flue-cured tobacco sample is with check plot substantially close within normal range (NR), no significant difference；Explanation
The anti-cryogen modulated by physiological activator of the invention has no adverse effects to flue-cured tobacco main components.Additionally, according to pilot scale
Point tobacco grower's reflection：Compared with check plot, anti-cryogen treatment region tobacco seedling growth gesture is more surging, and middle and upper part blade is slightly larger, leaves number
Many 3 or so, flowering time pusher 5 days or so of buddingging.
The anti-cryogen prepared using the embodiment of the present invention 3 and 4 carries out the test checking of embodiment 5,6,7,8, can also reach same
The good anti-cold effect of sample.
Claims (10)
1. one grow tobacco anti-cryogen, it is characterised in that be the solution comprising following density component：1.15~2.30g/L of glycine betaine,
5.0~10.0mg/L of salicylic acid, 3.0~5.0mg/L of abscisic acid, 600~1000mg/L of 5-ALA.
2. the anti-cryogen of tobacco as claimed in claim 1, it is characterised in that the component included in the anti-cryogen of tobacco is preferably dense
Degree：Glycine betaine 1.15g/L, salicylic acid 5.0mg/L, abscisic acid 3.0mg/L, 5- glycyl acetic acid 600mg/L.
3. the anti-cryogen of tobacco as claimed in claim 1, it is characterised in that also contain glycerine, Vc, calcium salt and trace element.
4. the anti-cryogen of tobacco as claimed in claim 3, it is characterised in that the calcium salt in the anti-cryogen of tobacco be nitrate or
One kind in calcium chloride, the concentration of calcium ion is 600~1000mg/L；The concentration of the Vc in the anti-cryogen of tobacco be 500~
1000mg/L, the concentration of glycerine are 15~20ml/L；5~10mg/L of trace elements iron, boron 5 are also included in every liter of anti-cryogen of tobacco
~10mg/L, 0.5~1.0mg/L of manganese, 10~25mg/L of zinc, 0.05~0.10mg/L of molybdenum.
5. the anti-cryogen of tobacco described in claim 1, it is characterised in that 0.5ml plant leaf blades are included in every liter of anti-cryogen of tobacco
One or more in surface active agent tween 20, Tween 80, triton x-100.
6. the preparation method of the anti-cryogen of tobacco described in claim 1 or 2, it is characterised in that step includes：
(1) component salicylic acid is dissolved in appropriate absolute ethyl alcohol or 95% ethanol, obtains solution I；
(2) component glycine betaine, 5-ALA are added to the water, are stirred continuously until fully dissolving obtains solution II；
(3) component abscisic acid is dissolved in appropriate absolute ethyl alcohol or 95% ethanol, obtains solution III；
(4) solution I and III are added in solution II, and add plant leaf blade surfactant, being stirred continuously makes it fully mix
Close uniform, the described anti-cryogen of tobacco is obtained.
7. the preparation method of the anti-cryogen of tobacco described in any one of Claims 1 to 5, it is characterised in that step includes：
(1) component salicylic acid is dissolved in appropriate absolute ethyl alcohol or 95% ethanol, obtains solution I；
(2) component glycine betaine, 5-ALA, Vc, glycerine, calcium salt and trace element are added to the water, are stirred continuously straight
Solution II is obtained to fully dissolving；
(3) component abscisic acid is dissolved in appropriate absolute ethyl alcohol or 95% ethanol, obtains solution III；
(4) solution I and III are added in solution II, and add plant leaf blade surfactant, being stirred continuously makes it fully mix
Close uniform, the described anti-cryogen of tobacco is obtained.
8. the application method of the anti-cryogen of tobacco described in any one of Claims 1 to 5, it is characterised in that the anti-cryogen of tobacco
In tobacco seedlings foliage-spray.
9. the application method of the anti-cryogen of tobacco according to claim 8, it is characterised in that tobacco seedlings go out before garden 5~7 days or
" also seedling stage ", the cloudy day of " stretching the root phase " breeding time or fine day dusk foliage-spray after tobacco seedlings field-transplanting.
10. the application method of the anti-cryogen of tobacco according to claim 8, it is characterised in that resisted using described tobacco cold
Foliage-spray, the every mu of amount of spraying are 25~30L after 3~5 times of dilutions of agent.
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| AU2017261240B2 (en) * | 2016-05-03 | 2020-11-19 | Valent Biosciences Llc | Methods of improving growth and stress tolerance in plants |
| CN106386808B (en) * | 2016-08-31 | 2019-03-22 | 四川龙蟒福生科技有限责任公司 | A kind of composition, preparation and its application |
| CN107517709B (en) * | 2017-09-14 | 2020-05-05 | 河南农业大学 | Tobacco planting method for preventing high-temperature induced maturity |
| CN107548866A (en) * | 2017-10-24 | 2018-01-09 | 黑龙江省科学院自然与生态研究所 | A kind of application of abscisic acid and application process |
| CN108947654B (en) * | 2018-07-19 | 2021-06-15 | 山东祥维斯生物科技股份有限公司 | Composite nutrition regulator for improving drought tolerance and cold resistance of cold-season turfgrass |
| CN109090118A (en) * | 2018-07-25 | 2018-12-28 | 广西大学 | A kind of tobacco strong sprout inducer and preparation method thereof |
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| CN109222192B (en) * | 2018-09-10 | 2021-07-13 | 云南省烟草农业科学研究院 | Flue-cured tobacco management and baking method for alleviating cold damage tobacco based on salicylic acid application |
| CN108720073B (en) * | 2018-09-10 | 2021-04-06 | 云南省烟草农业科学研究院 | Flue-cured tobacco management and baking method for alleviating cold damage tobacco based on betaine application |
| CN109574754A (en) * | 2018-12-29 | 2019-04-05 | 中化农业（临沂）研发中心有限公司 | Cold-resistant Water soluble fertilizer and preparation method thereof |
| CN111670906B (en) * | 2020-06-30 | 2021-07-30 | 京博农化科技有限公司 | Composition containing 5-aminolevulinic acid and S-abscisic acid |
| CN112679278A (en) * | 2020-12-29 | 2021-04-20 | 北京世纪阿姆斯生物工程有限公司 | Amino acid water-soluble fertilizer with function of enhancing low-temperature resistance of crops and preparation method thereof |
| CN113287506A (en) * | 2021-06-15 | 2021-08-24 | 河南农业大学 | Method for improving freezing resistance of tobacco seedlings |
| CN113519365B (en) * | 2021-06-16 | 2023-09-08 | 湖南省烟草公司常德市公司 | Cold-resistant growth-promoting composition and preparation for tobacco and application method of cold-resistant growth-promoting composition and preparation |
| CN113615691B (en) * | 2021-08-06 | 2022-06-17 | 南京农业大学 | Plant anti-refrigerant and preparation method and application thereof |
| CN116035005A (en) * | 2022-09-16 | 2023-05-02 | 中国烟草中南农业试验站 | Application of exogenous ABA in inhibiting low-temperature induced tobacco premature ejaculation |
| CN115812715A (en) * | 2022-11-27 | 2023-03-21 | 四川省烟草公司广元市公司 | Composition for improving stress resistance of tobacco |
| CN116548445B (en) * | 2023-05-10 | 2023-12-08 | 广东省科学院南繁种业研究所 | Preparation and method for improving cold resistance of tobacco |
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| ES2394476T3 (en) * | 2007-05-18 | 2013-02-01 | Sciessent, Llc | Food preservation compositions and procedures |
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