CN105181911A - Simulation scene culture system for determining oceanic primary productivity through black and white bottle method - Google Patents
Simulation scene culture system for determining oceanic primary productivity through black and white bottle method Download PDFInfo
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Abstract
The invention relates to a simulation scene culture system for determining oceanic primary productivity through a black and white bottle method. The simulation scene culture system comprises a culture unit, a temperature and water flow simulation control unit and an illumination simulation control unit. The culture unit comprises a plurality of culture subunits. The culture subunits comprise cylindrical water bath covers, a center rotating shaft is arranged along the center axes of the cylinders, culture bottle rotating supports are arranged on the periphery of the center rotating shaft, a plurality of culture bottles are detectably fixed to each culture bottle rotating support, and the culture subunits are arranged at intervals in a series connection mode with the center rotating shaft as the axis. The temperature and water flow simulation control unit comprises a circulating water bath pot, and the circulating water bath pot is connected with water inlets and water outlets in the water bath covers. The illumination simulation control unit comprises a light panel and a fluorescent tube, and the fluorescent tube is arranged on the light panel and connected with a power source. The illumination simulation control unit is arranged on the outer side of the outermost culture subunit.
Description
Technical field
The present invention relates to the culture systems of phytoplankton and primary productivity of marine ecosystem, be specifically related to a kind of simulated field culture systems utilizing black and white bottle oximetry to measure primary productivity of marine ecosystem.
Background technology
Primary productivity of marine ecosystem refers to that primary producer in ocean (mainly phytoplankton) produces organic ability or speed by photosynthesis or chemosynthesis, it is other heterotrophic existence bases in marine ecosystems, and fundamentally affects Global Biogeochemical Cycle and climate change.Therefore, the accurate test of primary productivity of marine ecosystem has become the important pivot of carbon cycle between various countries' research active organism and inorganic carbon reservior, the solid carbon ability in ocean and climate change.The method measuring marine phytoplankton primary productivity has a lot, at present ocean and lake most widely used be C-14 trace method and black and white bottle oximetry.Compared to C-14 trace method to experiment condition and the higher feature of equipment requirement, black and white bottle oximetry has the advantages such as operation is simple and easy, expense is low, no radioactivity pollute, uses so far from 20th century first half leaf always.And black and white bottle oximetry is by cultivating dissolved oxygen DO change in the water sample of front and back, and can calculate net primary productivity, gross primary productivity and respiration rate, this is that C-14 trace method is difficult to accomplish simultaneously.The party's ratio juris is based on photosynthesis reaction formula: H
2o+CO
2→ (CH
2o)
n+ O
2↑, the reversed reaction of this reaction is respiration; Owing to all there is certain equivalent relation between oxygen growing amount and organic substance growing amount or between zmount of oxygen consumption and organic substance consumption, so the change by measuring dissolved oxygen content in water body, can the growing amount of indirect calculation organic substance and consumption, and then calculate photosynthetic rate and respiration rate and gross primary productivity.
This technology can be divided into two kinds of methods in practical operation: " original position " in-situ batch culture method and " simulation " in-situ batch culture method, the former requires culture flask after adding water sample, be placed on predetermined depth under water, and require that cast anchor wait 24 hours (even longer time) until cultivate of research ship terminates, so high, the consuming time and effort of difficulty; The latter then carrys out simulated field condition by engineering, thus realizes the incubation of simulation original position.
Although there is the technical method of " simulation " in-situ batch culture in the past, there is the weak point of following several aspect in these technology:
(1) temperature accurately controlled in incubation is difficult to.Ocean water body temperature is in change all the time, and this not only shows the seasonal variations of large scale, also shows the mechanical periodicity that in a day, day alternates with night.And ocean water body has vertical three-dimensional, different water depth temperature is also different.Simulated culture technique in the past after being taken out by water sample, and be just again difficult to the temperature variation controlling water body, be more difficult to the temperature of degree of depth TWS being strict controlled in its script place.Research in the past shows, temperature is very big to primary production of phytoplankton and carbon assimilation index impacts, and this certainly will cause the error of primary productivity test result.
(2) intensity of illumination accurately controlled in incubation is difficult to.Illumination is the key factor affecting primary productivity of marine ecosystem, because illumination is phytoplankton produce the motive power of organic carbon by photosynthesis.Although partial simulation culture technique have employed the simulated solar illumination conditions such as daylight lamp in the past, be often difficult to the intensity of precision control illumination, the intensity of illumination being difficult to the former degree of depth with water sample is united; Even if part culture technique can adjust daylight lamp intensity of illumination, but owing to not being totally sealed environment, cause ambient natural light according to phytoplankton being produced to the impact being difficult to expect, and then cause the error of test result.
(3) analog physical hydrodynamic environment is difficult to.The ocean water body moment is in motion, and this physical motion has considerable influence to primary production of phytoplankton.Simulate culture technique in the past after water sample is taken out from deep-sea, be often again difficult to the ocean dynamical environment that simulation phytoplankton possesses originally.This will make surveyed primary productivity and legitimate reading have to be difficult to the deviation of expecting.
(4) be difficult to carry out simulation to the water sample of multiple water layer cultivate simultaneously.Ocean water body has vertical three-dimensional, and the illumination of different water depth, temperature and physical environment etc. are all different; Accordingly, the biomass of phytoplankton, species composition and physiological characteristic etc. also can change; Therefore, after obtaining water sample from the former depth of water, preferably can with the similar ecologic environment of the former depth of water in water sample is cultivated.And later stage zoning primary productivity, then need to obtain production force value to different water levels carries out integration on euphotic zone depth, and generally speaking obtained water layer production force value is more, and gained final area Primary Production force value is more accurate.But in practical operation, the finite space and the ocean water body dynamic environment of scientific surveying ship all bring great operation easier to multilayer Culture in situ.
(5) the accurate change that Accurate Determining cultivates dissolved oxygen DO in water sample is difficult to.The method that current mensuration dissolved oxygen DO is conventional has iodimetric titration (i.e. Winkler method), oxygen electrode method etc.Although the former accuracy of measurement is high, a kind of pure chemistry detection method, not only length consuming time, program are loaded down with trivial details, and need airtight culture flask to open sampling, just can observe the dissolved oxygen DO change in incubation.Oxygen electrode method is a kind of electrochemical detection method, can realize on-the-spot continuous coverage, has feature easily and fast.In addition, some spectrophotometric method newly developed and Fluorimetric Quenching Methods etc. are also had.Therefore, how by not only simple and efficient, simultaneously but the high oximetry methods of degree of accuracy be applied to deftly in practical operation and be also one and have difficult point to be solved.
In a word, though the culture technique utilizing oximetry to measure primary productivity of marine ecosystem can simulate 1-2 site environment parameter in the past, under the scientific surveying ship finite space and dynamic environment, perfect site physical chemical environment that is virtually reality like reality is difficult to; Be difficult to carry out multilayer water sample in-site modeling culture experiment; Be difficult to more accurate oximetry methods is applied in in-site modeling operation engineering perfectly, thus easily cause the error of measurement result.These are all that multiple analog culture technique was difficult to the difficult point of capturing in the past, and also the present invention makes the place broken through and improve exactly.
Summary of the invention
The present invention aims to provide one " simulation " in-situ batch culture system, with the problem such as solve large, the consuming time length of existing culture systems operation easier, operation requirements is high, test result is inaccurate; Simultaneously, make full use of the advantages such as black and white bottle oximetry is simple to operate, expense is low, no radioactivity pollute, perfection solves in scientific investigation shipboard measurement primary productivity of marine ecosystem and a difficult problem of carrying out related science experiment, for Marine Sciences investigation and research provide technical support.
Utilize light and dark bottle technique to measure a simulated field culture systems for primary productivity of marine ecosystem, comprise and cultivate unit, temperature and water flow simulation control module, lighting simulation control module; Cultivate unit and comprise several cultivation subelements, described cultivation subelement comprises the water-bath cover of cylindrical shape, cylindrical, center rotational shaft is set along axis place, center rotational shaft periphery arranges culture flask runing rest, culture flask runing rest removably fixes several culture flasks, described each cultivation subelement is that axis is arranged in series with center rotational shaft, and each cultivation between subelement exists gap; Described temperature and water flow simulation control module comprise recirculated water bath, and recirculated water bath is connected respectively with the water inlet on water-bath cover and water delivering orifice; Lighting simulation control module comprises optic panel, fluorescent tube, and fluorescent tube is arranged on optic panel, and fluorescent tube connects power supply, and lighting simulation control module is arranged on the outside being positioned at outermost cultivation subelement.
The quantity of described cultivation subelement is at least 2, and the water sample of the corresponding water layer of each water-bath cover, in actual use, determines to use several water-bath cover according to ocean water depth proper; If required water layer is more, center rotational shaft length can be increased, and the more water-bath covers of interpolation can solve.
In described each cultivation subelement, the quantity of culture flask is 6, and comprise 3 white culture flasks and 3 black culture flasks, white culture flask and black culture flask are intervally arranged.Described white culture flask is transparent material culture flask, and described black culture flask is lighttight culture flask, and appearance blacking or jealous glass material can be adopted to make.
Be that axis of symmetry is symmetrical arranged with center rotational shaft between described culture flask, make its trim, avoid unstable when rotated.
Described cultivation bottleneck is arranged on the outside of water-bath cover and is fixed on culture flask runing rest, facilitates the operations such as application of sample sampling, and there is not water in water-bath cover and infiltered the risk in culture flask by bottleneck.
Described culture flask is that angle is arranged, and namely bottleneck direction and body direction have angle, and unconventional direction is consistent.The bottleneck of culture flask is made into oblique, and is fixed on culture flask runing rest, in bottle, conveniently add the cleaning after water sample, also convenient cultivation; Cylindrical water-bath cover can be opened from right cylinder bottom surface and change culture flask, as adjusted the quantity of culture flask, changing the culture flask etc. of different penetrability, volume; And in incubation, water-bath cover and culture flask are all in closed state.
All place a dissolved oxygen DO sensing probe in described each culture flask, can the real time measure dissolved oxygen DO trace change; Sensing probe connection data line, and all data lines are integrated in the center rotational shaft of hollow, lean out in bottom by center rotational shaft, and data line is connected to dissolved oxygen DO detector or computer.Dissolved oxygen DO sensing probe is preferably arranged on the center of culture flask, and measurement result is more accurate.
Described water-bath cover adopts transparent material to make, and as glass, high-transmittance plastics etc., transparent material can not affect injecting of illumination.Water-bath cover can be fixed on center rotational shaft.
Described temperature and water flow simulation control module provide constant temperature current to reach the object of temperature control and simulated flow by recirculated water bath.Principle of control temperature is as follows: flow through water inlet from the thermostatted water of recirculated water bath outflow and be filled with transparent water steam-inflated plastic shroud fast, and rotate at water-bath cover Inner eycle, then returns recirculated water bath from water delivering orifice outflow.If temperature difference is little between water layer, by the thermostatted water current control temperature of mode same temperature that water-bath cover is connected; If temperature difference is comparatively large between water layer, then the constant temperature current by different temperatures provide each self-corresponding water layer temperature for each water-bath cover.Simulated flow principle is as follows: the constant temperature current that recirculated water bath produces inject closed circular non-opaque water-bath cover fast from water inlet, thus drive culture flask to rotate, culture flask and then drive culture flask runing rest, thus all culture flasks and runing rest rotate around the transparent light shield Inner eycle of center rotational shaft in circle.By controlling the speed injecting current, control velocity of rotation; Thus analog physical hydrodynamic condition and wave, current are on the disturbance of phytoplankton ecology environment and impact.If the flow dynamic difference between each water layer is not too large, then a circulator bath system can be used to control the velocity of rotation of culture flask by series system; If hydrodynamic condition difference is comparatively large, then can provide separately the constant temperature current of friction speed for different water-bath covers, thus the at utmost former water layer hydrodynamic environment of simulation.
Therefore, the temperature in each water-bath cover and current can control by same recirculated water bath is unified or is controlled respectively by different recirculated water bath respectively.
Described water inlet and water delivering orifice are arranged on the side of cylindrical water-bath cover, and just to the rotational trajectory of culture flask, both, at a distance of more far away better, make the water injected from recirculated water bath circulate completely in water-bath cover.
Described optic panel is parallel with the bottom surface of cylindrical water-bath cover, makes to be positioned at the intensity of illumination that each culture flask of same cultivation subelement receives identical.
Arrange light quantum meter at each water-bath cover to the side of lighting simulation control module, water-bath cover is close to by light quantum meter, and be positioned at the center of circle, cylindrical water-bath cover bottom surface be the center of circle, the center of circle to the culture flask middle part distance circle that is radius circumferentially.Light quantum meter is connected with computer, for detecting light intensity in real time, guarantees that Optical power values is accurate.
Between described cultivation subelement, arrange a filter, filter adopts blue tinted glass or the plastics with certain transparency, as mist blue material (MistBlue, LeeFiltersNo.061) etc.; Filter, can in conjunction with the monitor value of light quantum meter to light intensity for controlling the intensity of illumination being irradiated to different water levels culture flask, make to be irradiated to light intensity on culture flask and former water layer completely the same.
Between described fluorescent tube and power supply, current controller and/or timer can also be set.
Described cultivation unit is fixed on support after center rotational shaft series connection, and the cultivation subelement of series connection can longitudinally be arranged also can horizontally set.
Described culture systems is all arranged in lighttight light shield except recirculated water bath.For avoiding the interference of natural light outside line, adopt totally enclosed lighttight outside light shield, and light shield side uses and light tight and airtight draws curtain, so both can play the effect of closed illumination in incubation, also can draw the curtain apart before and after cultivation and carry out application of sample or washing.Outside light shield adopts rigid, when rocking on scientific surveying ship like this or move, can protect the transparent water steam-inflated plastic shroud of the inside.The reason that recirculated water bath is placed on outside light shield is: one is the space saving light shield, and two is be placed on outside easily heat radiation, and three is handled easilies.
Described light shield adopts rigid, and body is tetragonal body, and therefore can conveniently add up stacks; Meanwhile, if the water sample obtained for the sampling website of diverse geographic location is cultivated, can be stacked together by this kind equipment multiple, and then save space shared on research ship.
Compared to prior art, beneficial effect of the present invention is embodied in:
(1) for being difficult to the shortcoming accurately controlling temperature in incubation, temperature simulation control module of the present invention adopts circular non-opaque water-bath cover to be that culture flask provides a totally-enclosed culture environment, and by the constant temperature current that circulator bath device drives flows continuously through from culture flask, temperature control error only ± 0.1 DEG C; And the temperature control scope of water-bath, between 0 DEG C-100 DEG C, covers the viable temperature range of nearly all marine phytoplankton.Therefore, the present invention can not only the water temperature of accurate analog water sample former degree of depth in ocean, and is applicable to global any maritime waters.
(2) for being difficult to the shortcoming accurately controlling intensity of illumination in incubation, lighting simulation control module of the present invention adopts current intensity controller and timer to control fluorescent tube and optic panel, thus strictly control intensity of illumination and illumination mode, and by the further close inspection light intensity of light quantum meter, guarantee light intensity and water sample completely the same in the light intensity of former ocean water body; And culture flask and the cylindrical water-bath cover outside it are high transparency material, can not affect ght transmission, can ideally simulate ocean intensity of illumination.In addition, what the present invention was the most key is a bit take outside light shield whole culture device (except circulator bath equipment) to be all closed, to effectively reduce in surrounding environment natural lighting like this to the impact of culture flask, thus make test result more accurate.
(3) for the shortcoming being difficult to analog physical hydrodynamic environment, the water jet propulsion culture flask that the present invention utilizes circulator bath to drive and runing rest continue to rotate around center rotational shaft, thus simulate ocean water body physical motion; And culture flask this under flow action not only the passive current that accept bring thermostatic effect, and serving the effect of runner of promoting, is kill two birds with one stone.Water sample in bottle owing to rotating before and after culture flask or up and down, under the effect of centrifugal force or natural gravity, can left and right mixing or move up and down.In addition, control by the flow velocity of regulating thermostatic current the speed that culture flask rotates, thus at utmost simulation ocean dynamical environment.
(4) carry out simulating the shortcoming of cultivating to the water sample of multiple water layer for being difficult to, the while that the present invention adopting multiple cultivation subelement to be cascaded, synchronously simulating is cultivated simultaneously.In illumination, cultivate between subelements for every two, all adopt filter to control the intensity of illumination being irradiated to different water levels culture flask, and by light quantum meter close inspection intensity of illumination, make to be irradiated to light intensity on culture flask and former water layer completely the same; In temperature control, if temperature difference is little between water layer, then the mode thermostatted water current control temperature by water-bath cover is connected; If temperature difference is comparatively large between water layer, then provide the water bath with thermostatic control current of different temperatures for different water-bath covers.Similar, in Simulated Water power, also can adopt series system, or different water-bath water intensity of flows is provided separately.
(5) for the shortcoming being difficult to Accurate Determining and cultivating the accurate change of dissolved oxygen DO in water sample, the present invention adopts the mode of placing dissolved oxygen DO sensing probe in bottle, can the trace change of dissolved oxygen DO in Real-Time Monitoring culture flask, and the data measured popping one's head in are by data line real-time Transmission to external computer; The interface of sensing probe and data line is fixed on bottle cap, and guarantees probe and bottle cap and seal completely between bottle cap and culture flask; Change culture flask bottleneck into italic mouth and be fixed on runing rest, application of sample, sampling and washing can be facilitated like this.
Accompanying drawing explanation
Fig. 1 culture systems structural representation of the present invention;
Fig. 2 the present invention cultivates sub-unit structure schematic diagram;
Fig. 3 lighting simulation control module structural representation.
In figure: 1-cultivates bottleneck; 2-light shield; 3-water inlet; 4-filter; 5-recirculated water bath; The black culture flask of 7-; 8-dissolved oxygen DO sensing probe; 9-support; 10-optic panel; 11-water delivering orifice; 12-water-bath cover; The white culture flask of 13-; 14-data line; 15-center rotational shaft; 16-culture flask runing rest; 17-light quantum meter; 18-computer; 19-fluorescent tube; 20-current controller; 21-timer; 22-power supply.
In Fig. 2, arrow represents water (flow) direction.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further details.
The light and dark bottle technique that utilizes as shown in Figure 1-2 measures the simulated field culture systems of primary productivity of marine ecosystem, comprises and cultivates unit, temperature and water flow simulation control module, lighting simulation control module.
Cultivate unit and comprise 5 cultivation subelements, each cultivation subelement comprises the water-bath cover 12 of cylindrical shape, cylindrical, center rotational shaft 15 is set along axis place, center rotational shaft 15 periphery arranges culture flask runing rest 16, culture flask runing rest 16 is removably fixed 6 culture flasks, comprise 3 white culture flasks 13 and 3 black culture flasks 7, white culture flask 13 and black culture flask 7 are intervally arranged, and with center rotational shaft 15 for axis of symmetry is symmetrical arranged.All culture flasks are angle and arrange, and cultivate the outside that bottleneck 1 is arranged on water-bath cover 12.Water-bath cover 12 sets into the mouth of a river 3 and water delivering orifice 11, on the side that water inlet 3 is arranged on cylindrical water-bath cover 12 and just to the rotational trajectory of culture flask, water delivering orifice 11 is arranged on the side relative with water inlet 3.
Each cultivation subelement is with center rotational shaft 15 for axis is arranged in series, and each cultivation between subelement exists gap, thus can not have influence on the operation to each cultivation subelement.
In each culture flask, all place a dissolved oxygen DO sensing probe 8, dissolved oxygen DO sensing probe 8 is arranged on the center of culture flask, can the real time measure dissolved oxygen DO trace change; Sensing probe 8 connection data line, and all data lines 14 are integrated in the center rotational shaft 15 of hollow, lean out in bottom by center rotational shaft 15, and data line 14 is connected to computer 18.
Water-bath cover 12 adopts high-transmittance plastics to make, and can not affect injecting of illumination, and light weight is non-friable, convenient carrying.
Temperature and water flow simulation control module comprise recirculated water bath 5, because between water layer each in the present embodiment, temperature and flow dynamic are more or less the same, therefore adopt the mode of being connected by water-bath cover 12 to come control temperature and current with same recirculated water bath 5.Recirculated water bath 5 is connected with the water inlet 3 of first water-bath cover 12, the water delivering orifice 11 of first water-bath cover 12 is connected with the water inlet 3 of second water-bath cover 12, the water delivering orifice 11 of second water-bath cover 12 is connected with the water inlet 3 of the 3rd water-bath cover 12, the water delivering orifice 11 of the 3rd water-bath cover 12 is connected with the water inlet 3 of the 4th water-bath cover 12, the water delivering orifice 11 of the 4th water-bath cover 12 is connected with the water inlet 3 of the 5th water-bath cover 12, and the water delivering orifice 11 of the 5th water-bath cover 12 is connected with recirculated water bath 5.Temperature control process is as follows: the water inlet 3 flowing through first water-bath cover 12 from the thermostatted water of recirculated water bath 5 outflow enters the first cultivation subelement, and enter follow-up cultivation subelement successively, constant temperature current rotate at each water-bath cover 12 Inner eycle, finally flow out from the water delivering orifice 11 of the 5th water-bath cover and return recirculated water bath 5.Constant temperature current, while temperature control, drive culture flask to rotate, culture flask and then driving culture flask runing rest 16, thus all culture flasks and runing rest 16 rotate around center rotational shaft 15 at tubular transparent water steam-inflated plastic shroud 12 Inner eycle.By controlling the speed injecting current, control culture flask velocity of rotation; Thus analog physical hydrodynamic condition and wave, current are on the disturbance of phytoplankton ecology environment and impact.
Lighting simulation control module is as shown in Figure 3 arranged on the outside being positioned at outermost cultivation subelement, comprise optic panel 10, fluorescent tube 19, fluorescent tube 19 is arranged on optic panel 10, and fluorescent tube 19, current controller 20, timer 21 are connected successively with power supply 22.
Optic panel 10 is parallel with the bottom surface of cylindrical water-bath cover 12, makes to be positioned at the intensity of illumination that each culture flask of same cultivation subelement receives identical.
At each water-bath cover 12, light quantum meter 17 is set towards the side of lighting simulation control module, water-bath cover 12 is close to by light quantum meter 17, and be positioned at the center of circle, cylindrical water-bath cover 12 bottom surface be the center of circle, the center of circle to the culture flask middle part distance circle that is radius circumferentially.Light quantum meter 17 is connected with computer, for detecting light intensity at any time, guarantees that Optical power values is accurate.
Cultivate between subelement at every two, arrange a filter 4, filter 4 adopts mist blue material (MistBlue), and model is LeeFiltersNo.061; Filter 4 for controlling the intensity of illumination being irradiated to different water levels culture flask, in conjunction with the mensuration of light quantum meter 17 pairs of light intensity, make to be irradiated to light intensity on culture flask and former water layer completely the same.
Whole cultivation unit is fixed on support 9, have employed the mode longitudinally arranged by the cultivation subelement of each series connection in the present embodiment.
This culture systems is all arranged in lighttight light shield 2 except recirculated water bath.For avoiding the interference of natural light outside line, adopt totally enclosed lighttight outside light shield 2, and light shield 2 side uses and light tight and airtight draws curtain, so both can play the effect of closed illumination in incubation, also can draw the curtain apart after incubation to sample.Outside light shield 2 adopts rigid, when rocking on scientific surveying ship like this or move, can protect the transparent water steam-inflated plastic shroud 12 of the inside.The body of light shield 2 is tetragonal body, and therefore can conveniently add up stacks; Meanwhile, if the water sample obtained for the sampling website of diverse geographic location is cultivated, can be stacked together by this kind equipment multiple, and then save space shared on research ship.The reason that recirculated water bath is placed on outside light shield 2 is: one is the space saving light shield 2, and two is be placed on outside easily heat radiation, and three is handled easilies.
Claims (10)
1. utilize light and dark bottle technique to measure a simulated field culture systems for primary productivity of marine ecosystem, it is characterized in that, comprise and cultivate unit, temperature and water flow simulation control module, lighting simulation control module; Cultivate unit and comprise several cultivation subelements, described cultivation subelement comprises the water-bath cover (12) of cylindrical shape, cylindrical, center rotational shaft (15) is set along axis place, center rotational shaft (15) periphery arranges culture flask runing rest (16), (16) removably fix several culture flasks to culture flask runing rest, described each cultivation subelement is with center rotational shaft (15) for axis is arranged in series, and each cultivation between subelement exists gap; Described temperature and water flow simulation control module comprise recirculated water bath (5), and recirculated water bath (5) is connected respectively with the water inlet (3) on water-bath cover (12) and water delivering orifice (11); Lighting simulation control module comprises optic panel (10), fluorescent tube (19), fluorescent tube (19) is arranged on optic panel (10), fluorescent tube (19) connects power supply (22), and lighting simulation control module is arranged on the outside being positioned at outermost cultivation subelement.
2. the simulated field culture systems utilizing light and dark bottle technique to measure primary productivity of marine ecosystem according to claim 1, it is characterized in that, in described each cultivation subelement, the quantity of culture flask is 6, comprise 3 white culture flasks (13) and 3 black culture flasks (7), white culture flask (13) and black culture flask (7) are intervally arranged, between culture flask with center rotational shaft (15) for axis of symmetry is symmetrical arranged.
3. the simulated field culture systems utilizing light and dark bottle technique to measure primary productivity of marine ecosystem according to claim 1 and 2, it is characterized in that, described culture flask is that angle is arranged, and cultivates bottleneck (1) and is arranged on the outside of water-bath cover (12) and is fixed on culture flask runing rest (16).
4. the simulated field culture systems utilizing light and dark bottle technique to measure primary productivity of marine ecosystem according to claim 1 and 2, it is characterized in that, all place a dissolved oxygen DO sensing probe (8) in described each culture flask, dissolved oxygen DO sensing probe (8) is arranged on the center of culture flask; Dissolved oxygen DO sensing probe (8) connection data line (14), and all data lines (14) are integrated in the center rotational shaft (15) of hollow, lean out in bottom by center rotational shaft (15), and data line (14) is connected to dissolved oxygen DO detector or computer (18).
5. the simulated field culture systems utilizing light and dark bottle technique to measure primary productivity of marine ecosystem according to claim 1 and 2, it is characterized in that, described water inlet (3) and water delivering orifice (11) are arranged on the side of cylindrical water-bath cover (12), just to the rotational trajectory of culture flask.
6. the simulated field culture systems utilizing light and dark bottle technique to measure primary productivity of marine ecosystem according to claim 1 and 2, it is characterized in that, described optic panel (10) is parallel with the bottom surface of cylindrical water-bath cover (12).
7. the simulated field culture systems utilizing light and dark bottle technique to measure primary productivity of marine ecosystem according to claim 1 and 2, it is characterized in that, each water-bath cover (12), light quantum meter (17) is set towards the side of lighting simulation control module, water-bath cover (12) is close to by light quantum meter (17), and be positioned at cylindrical water-bath cover (12) center of circle, bottom surface be the center of circle, the center of circle to culture flask middle part distance for radius circle circumferentially, light quantum meter (17) is connected with computer.
8. the simulated field culture systems utilizing light and dark bottle technique to measure primary productivity of marine ecosystem according to claim 7, is characterized in that, between described cultivation subelement, arrange a filter (4).
9. the simulated field culture systems utilizing light and dark bottle technique to measure primary productivity of marine ecosystem according to claim 1 and 2, it is characterized in that, current controller (20) and/or timer (21) are also set between described fluorescent tube (19) and power supply (22).
10. the simulated field culture systems utilizing light and dark bottle technique to measure primary productivity of marine ecosystem according to claim 1 and 2, it is characterized in that, described culture systems is all arranged in lighttight light shield (2) except recirculated water bath (5), and side of light shield (2) uses and light tight and airtight draws curtain, light shield (2) adopts rigid, and body is tetragonal body.
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| CN105954240A (en) * | 2016-05-23 | 2016-09-21 | 中国科学院南京地理与湖泊研究所 | Measuring device and measuring method for in-situ quick determination of primary productivity of lakes |
| CN111830211A (en) * | 2020-07-30 | 2020-10-27 | 中国水产科学研究院南海水产研究所 | RS-based ocean primary productivity distribution visualization method |
| CN117192058A (en) * | 2023-09-07 | 2023-12-08 | 中国科学院南海海洋研究所 | Online monitoring device for carbon source sink of aquatic ecosystem and water body detection method |
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| CN117192058A (en) * | 2023-09-07 | 2023-12-08 | 中国科学院南海海洋研究所 | Online monitoring device for carbon source sink of aquatic ecosystem and water body detection method |
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