CN105181911B - The simulation in-situ batch culture system of primary productivity of marine ecosystem is determined using light and dark bottle technique - Google Patents
The simulation in-situ batch culture system of primary productivity of marine ecosystem is determined using light and dark bottle technique Download PDFInfo
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Abstract
The present invention relates to the simulation in-situ batch culture system that a kind of utilization black and white bottle test sample method determines primary productivity of marine ecosystem, including culture unit, temperature and water flow simulation control unit, lighting simulation control unit；Cultivating unit includes several culture subelements, the culture subelement includes the water-bath cover of cylindrical shape, center rotational shaft is set along axis in cylinder, center rotational shaft periphery sets blake bottle runing rest, several blake bottles are removably secured on blake bottle runing rest, each culture subelement is arranged in series by axis of center rotational shaft, there is gap between each culture subelement；Temperature and the water flow simulation control unit includes recirculated water bath, and recirculated water bath is connected respectively with the water inlet and delivery port on water-bath cover；Lighting simulation control unit includes optic panel, fluorescent tube, and fluorescent tube is arranged on optic panel, and fluorescent tube connection power supply, lighting simulation control unit is arranged in the outside of outermost culture subelement.
Description
Technical field
The present invention relates to phytoplankton and the culture systems of primary productivity of marine ecosystem, and in particular to one kind is surveyed using black and white bottle
Oxygen method determines the simulation in-situ batch culture system of primary productivity of marine ecosystem.
Background technology
Primary productivity of marine ecosystem refers to primary producer in ocean (mainly phytoplankton) by photosynthesis or chemistry
The ability or speed of synthetically produced organic matter, it is the existence basis of other heterotrophic organisms in marine ecosystems, and from basic
On affect Global Biogeochemical Cycle and climate change.Therefore, the accurate test of primary productivity of marine ecosystem has turned into each
The important pivot of carbon cycle, ocean carbon sequestration capacity and climate change between state's research active organism and inorganic carbon reservior.Survey
The method of Dinghai ocean primary production of phytoplankton has a lot, and most widely used in ocean and lake at present is C-14 trace methods
With black and white bottle oximetry.The characteristics of compared to C-14 trace methods to experiment condition and higher equipment requirement, black and white bottle oximetry tool
Have the advantages that operation is simple, expense is low, "dead" pollution, from 20th century first half leaf always using so far.Moreover, black and white bottle is surveyed
Oxygen method is changed by dissolved oxygen in water sample before and after culture, and net primary productivity, gross primary productivity can be calculated simultaneously and is exhaled
Activity ratio is inhaled, this is that C-14 trace methods are difficult to.The principle of this method is to react formula based on photosynthesis：H2O+CO2→
(CH2O)n+O2↑, the back reaction of the reaction is respiration；Due between oxygen growing amount and organic substance growing amount or oxygen disappears
There is certain equivalent relation between consumption and organic substance consumption, so the change by determining dissolved oxygen content in water body
Change, the growing amount and consumption of organic substance can be calculated indirectly, and then calculate photosynthetic rate and respiration rate and total
Primary productivity.
The technology can be divided into two methods in practical operation：" original position " in-situ batch culture method and " simulation " in-situ batch culture method,
The former requires blake bottle being placed on predetermined depth under water after addition water sample, and requires that investigating secure waits 24 hours
(or even longer time) is until culture terminates, so difficulty is high, time-consuming and laborious；And the latter is then simulated now by engineering technology
Field condition, so as to realize simulation incubation in situ.
Although there is the technical method of " simulation " in-situ batch culture in the past, these technologies are present following aspects not
Foot part：
(1) it is difficult to the temperature in accurate control incubation.Ocean water body temperature is all the time in change, and this is not only showed
In the seasonal variations of large scale, the mechanical periodicity that day alternates with night in one day is also manifested by.Moreover, ocean water body has vertical solid
Dimension, different water depth temperature is also different.Simulation culture technique was just again difficult to control to the temperature of water body after water sample is taken out in the past
Degree change, it is more difficult to the temperature for the depth being strict controlled in TWS where its script.Research showed in the past, and temperature is to floating
Swim vegetative primary productivity and carbon assimilation index impacts greatly, this certainly will cause the error of primary productivity test result.
(2) it is difficult to the intensity of illumination in accurate control incubation.Illumination be influence primary productivity of marine ecosystem it is crucial because
Element, because illumination is the motive power that phytoplankton produces organic carbon by photosynthesis.Although conventional partial simulation culture technique
The simulated solar illumination condition such as fluorescent lamp is employed, but is often difficult to the intensity of precision control illumination, it is difficult to be former deep with water sample
The intensity of illumination of degree is united；Even if part culture technique can adjust fluorescent lamp intensity of illumination, but due to not being fully sealed
Environment, cause ambient natural light to shine to produce phytoplankton and be difficult to expected influence, and then cause the error of test result.
(3) it is difficult to analog physical hydrodynamic environment.The ocean water body moment is in motion, and this physical motion is to swimming
Vegetative primary productivity has considerable influence.Simulation culture technique was often again difficult after water sample is taken out from deep-sea in the past
To simulate the ocean dynamical environment that phytoplankton possesses originally.This will make it that surveyed primary productivity and legitimate reading are in distress
With expected deviation.
(4) it is difficult to carry out simulation culture to the water sample of multiple water layers simultaneously.Ocean water body has vertical three-dimensional, different
Illumination, temperature and physical environment of the depth of water etc. are different；Accordingly, the biomass of phytoplankton, species composition and physiological characteristic
Deng can also change；Therefore, obtained deeply after water sample from raw water, it is therefore desirable to water sample is entered in ecological environment deeply similar with raw water
Row culture.And later stage zoning primary productivity, then need to different water levels obtain production force value it is enterprising in euphotic zone depth
Row integration, it is however generally that obtain water layer produce force value it is more, gained final area Primary Production force value is more accurate.However, in reality
In the operation of border, the confined space and ocean water body dynamic environment of scientific surveying ship bring great behaviour to multilayer Culture in situ
Make difficulty.
(5) it is difficult to the accurate change of dissolved oxygen in Accurate Determining culture water sample.Determining the conventional method of dissolved oxygen at present has
Iodimetric titration (i.e. Winkler methods), oxygen electrode method etc..Although the former accuracy of measurement is high, a kind of pure chemistry detection method,
Not only time-consuming, program is cumbersome, and needs closed blake bottle opening sampling, can just observe the dissolved oxygen in incubation
Change.Oxygen electrode method is a kind of electrochemical detection method, and the continuous measurement in scene can be achieved, with easily and fast the characteristics of.This
Outside, there are some AASs newly developed and Fluorimetric Quenching Method etc..Therefore, how will it is not only simple and efficient, while but also accurate
The high oximetry methods of degree, which are deftly applied to be also one in practical operation, difficult point to be solved.
In a word, though the culture technique for determining primary productivity of marine ecosystem using oximetry in the past can simulate 1-2 site environment
Parameter, but it is difficult under the scientific surveying ship confined space and dynamic environment the perfect real site physical chemical environment of simulation；It is difficult
To carry out multilayer water sample in-site modeling culture experiment；It is difficult to being applied to more accurate oximetry methods into in-site modeling operation perfectly
In engineering, so as to be easily caused the error of measurement result.These are all the difficult points that conventional multiple analog culture technique is difficult to capture,
Also exactly the present invention makes breakthrough and improved place.
The content of the invention
The present invention is intended to provide a kind of " simulation " in-situ batch culture system, to solve, existing culture systems operation difficulty is big, consume
The problems such as duration, operation require high, test result is inaccurate；Meanwhile, making full use of black and white bottle, oximetry is simple to operate, expense
The advantages of low, "dead" pollution, perfection solves in scientific investigation shipboard measurement primary productivity of marine ecosystem and carries out related science
The problem of experiment, is investigated for Marine Sciences and research provides technical support.
A kind of utilization light and dark bottle technique determines the simulation in-situ batch culture system of primary productivity of marine ecosystem, including culture unit, temperature
Degree and water flow simulation control unit, lighting simulation control unit；Cultivating unit includes several culture subelements, culture
Unit includes the water-bath cover of cylindrical shape, and center rotational shaft is set along axis in cylinder, and center rotational shaft periphery is set
Several blake bottles are removably secured on blake bottle runing rest, blake bottle runing rest, each culture subelement is in
Centre rotating shaft is arranged in series for axis, there is gap between each culture subelement；Temperature and the water flow simulation control unit includes
Recirculated water bath, recirculated water bath is connected respectively with the water inlet and delivery port on water-bath cover；Lighting simulation control unit includes
Optic panel, fluorescent tube, fluorescent tube are arranged on optic panel, and fluorescent tube connection power supply, lighting simulation control unit is set
In the outside positioned at outermost culture subelement.
The quantity of the culture subelement is at least two, the water sample of one water layer of each water-bath cover correspondence, in actual use
In, determined according to ocean water depth proper using several water-bath covers；If required water layer is more, in can increasing
Entreating shaft length, and add more water-bath covers to solve.
The quantity of blake bottle is 6 in each culture subelement, including 3 white blake bottles and 3 black blake bottles, in vain
Blake bottle and black blake bottle are intervally arranged.The white blake bottle is transparent material blake bottle, and the black blake bottle is lighttight
Blake bottle, can be made using appearance blacking or jealous glass material.
It is symmetrical arranged between the blake bottle by symmetry axis of center rotational shaft, makes its trim, it is to avoid is unstable when rotated.
The culture bottleneck is arranged on the outside of water-bath cover and is fixed on blake bottle runing rest, convenient sample-adding sampling etc.
Operation, and risk in blake bottle is infiltered by bottleneck in the absence of water-bath cover reclaimed water.
The blake bottle is set for angle, i.e., bottleneck direction has angle with body direction, and unconventional direction is consistent.
The bottleneck of blake bottle is made into oblique, and be fixed on blake bottle runing rest, it is convenient that water sample is added into bottle, culture is also convenient for
Cleaning afterwards；Cylindrical water-bath cover can be opened to change blake bottle from cylinder bottom surface, such as quantity of adjustment blake bottle,
Change blake bottle of different light transmittances, volume etc.；And in incubation, water-bath cover and blake bottle are in closed state.
A dissolved oxygen sensing probe is placed in each blake bottle, can the real time measure dissolved oxygen micro change；Pass
Sense probe connection data wire, and all data wires are integrated in hollow center rotational shaft, leant out by center rotational shaft in bottom
Come, and data wire is connected to dissolving oxygen probe or computer.Dissolved oxygen sensing probe is preferably provided at the centre bit of blake bottle
Put, measurement result is more accurate.
The water-bath cover is made of transparent material, such as glass, high-transmittance plastics, and transparent material does not interfere with illumination
Injection.Water-bath cover can be fixed on center rotational shaft.
Described temperature and water flow simulation control unit provides constant temperature current to reach temperature control and mould by recirculated water bath
Intend the purpose of current.Principle of control temperature is as follows：The thermostatted water stream flowed out from recirculated water bath by water inlet is quickly filled with transparent water
Steam-inflated plastic shroud, and rotated in water-bath cover interior circulation, then return to recirculated water bath from delivery port outflow.If temperature difference is not between water layer
Greatly, the constant temperature flow cavitation result temperature of same temperature can be used by way of water-bath cover is connected；If temperature phase between water layer
Difference is larger, then can provide each self-corresponding water layer temperature by the constant temperature current of different temperatures for each water-bath cover.Simulation water
Flow principle as follows：The constant temperature current that recirculated water bath is produced are rapidly injected the circular non-opaque water-bath cover of closing from water inlet, so that
Blake bottle is driven to rotate, blake bottle and then driving blake bottle runing rest, so that all blake bottles and runing rest are around center
Rotating shaft is rotated in circular transparent light shield interior circulation.By controlling the speed of injection current, to control velocity of rotation；So as to
The disturbance and influence of analog physical hydrodynamic condition and wave, current on phytoplankton ecology environment.Such as the water between each water layer
Mobilization force difference is not too big, then can control the rotation speed of blake bottle using a circulator bath system by series system
Degree；If hydrodynamic condition difference is larger, the constant temperature current of friction speed can be provided separately for different water-bath covers, so that
At utmost simulation raw water layer hydrodynamic environment.
Therefore, the temperature and current in each water-bath cover can be uniformly controlled or respectively by difference by same recirculated water bath
Recirculated water bath is controlled respectively.
The water inlet is arranged on the side of cylindrical water-bath cover with delivery port, just the rotational trajectory to blake bottle,
Both make to circulate completely in water-bath cover from the water that recirculated water bath injects at a distance of more remote better.
The optic panel is parallel with the bottom surface of cylindrical water-bath cover, makes to be located at each blake bottle in same culture subelement and connects
The intensity of illumination being subject to is identical.
Light quantum meter is set to the side of lighting simulation control unit in each water-bath cover, light quantum meter is close to water-bath
Cover, and positioned at by the center of circle of the cylindrical water-bath cover bottom surface center of circle, the center of circle to blake bottle middle part distance be the circle of radius
On circumference.Light quantum meter is connected with computer, for detecting luminous intensity in real time, to ensure that Optical power values are accurate.
Between the culture subelement, a filter is set, filter uses the blue tinted glass with certain transparency
Or plastics, such as mist blue material (Mist Blue, Lee Filters No.061) etc.；Filter is irradiated to different water for control
The intensity of illumination of layer blake bottle, can combine light quantum meter to the monitor value of light intensity, make to be irradiated to the light intensity and raw water on blake bottle
Layer is completely the same.
Current controller and/or timer can also be set between the fluorescent tube and power supply.
The culture unit is fixed on support after being connected through center rotational shaft, and the culture subelement of series connection can be with longitudinally disposed
Can also laterally it set.
The culture systems are arranged in addition to recirculated water bath in lighttight light shield.To avoid natural light outside line
Interference, used using a side of totally enclosed lighttight outside light shield, and light shield light tight and closed
Curtain is drawn, the effect of closing illumination so can have both been played in incubation, can also draw the curtain apart to be added before and after culture
Sample or washing.Outside light shield uses rigid, when so rocking or move on scientific surveying ship, can protect the inside
Transparent water steam-inflated plastic shroud.The reason for recirculated water bath is placed on outside light shield is：One is the space for saving light shield, and two are placed on outside
Easily radiating, three be convenient operation.
The light shield uses rigid, and body is tetragonal body, therefore conveniently cumulative can stack；Meanwhile, if pin
The water sample that the sampling website of diverse geographic location is obtained is cultivated, can be stacked together with this multiple kind equipment, and then
Save shared space on research ship.
Compared to prior art, beneficial effects of the present invention are embodied in：
(1) for the shortcoming for being difficult to accurately control temperature in incubation, temperature simulation control unit of the invention is used
Circular non-opaque water-bath cover provides a totally-enclosed culture environment for blake bottle, and by circulator bath device drives from blake bottle
The constant temperature current flowed continuously through outside, only ± 0.1 DEG C of temperature control error；Moreover, the temperature control scope of water-bath is covered between 0 DEG C -100 DEG C
The viable temperature range of nearly all marine phytoplankton institute is covered.Therefore, the present invention can not only accurately simulated water sample in sea
The water temperature of foreign original depth, and suitable for global any maritime waters.
(2) for the shortcoming for being difficult to accurately control intensity of illumination in incubation, lighting simulation control unit of the invention
Using current intensity controller and timer control fluorescent tube and optic panel, so as to strictly control intensity of illumination and illumination mould
Formula, and pass through the further close inspection luminous intensity of light quantum meter, it is ensured that the luminous intensity of luminous intensity and water sample in former ocean water body
It is completely the same；Moreover, blake bottle is high transparency material with the cylindrical water-bath cover outside it, light transmission is not interfered with,
Can perfect simulation ocean intensity of illumination.In addition, it is a little to take outside light shield entirely to cultivate that the present invention is the most key
Equipment (except circulator bath equipment) is all closed, and so effectively reduces in surrounding environment natural lighting to blake bottle
Influence, so that test result is more accurate.
(3) shortcoming for being difficult to analog physical hydrodynamic environment is directed to, the current that the present invention is driven using circulator bath are pushed away
Dynamic blake bottle and runing rest are persistently rotated around center rotational shaft, so as to simulate ocean water body physical motion；And blake bottle sheet
Not only passively receive current under flow action and bring thermostatic effect, and serve the effect for promoting runner, be at one stroke
Two.Water sample in bottle is due to before and after blake bottle or rotating upwardly and downwardly, in the presence of centrifugal force or natural gravity, can left and right it is mixed
Close or move up and down.In addition, the speed that blake bottle is rotated is controlled by the flow velocity of regulating thermostatic current, so that farthest
Simulate ocean dynamical environment.
(4) for being difficult to simulate the shortcoming of culture to the water sample of multiple water layers simultaneously, the present invention uses multiple cultures
Subelement is cascaded while synchronously simulating culture.In terms of illumination, between each two culture subelement, using filter
To control the intensity of illumination for being irradiated to different water levels blake bottle, and by light quantum meter close inspection intensity of illumination, make to be irradiated to
Light intensity and raw water layer on blake bottle is completely the same；In terms of temperature control, if temperature difference less, can lead between water layer
Mode that water-bath cover is connected is crossed to use constant temperature flow cavitation result temperature；If temperature difference is larger between water layer, for difference
Water-bath cover provide different temperatures water bath with thermostatic control current.Similar, in terms of hydrodynamic force is simulated, it would however also be possible to employ series connection side
Formula, or different water-bath flow intensities are provided separately.
(5) shortcoming for being difficult to the accurate change of dissolved oxygen in Accurate Determining culture water sample is directed to, the present invention is using in bottle
The mode of dissolved oxygen sensing probe is placed, the micro change of dissolved oxygen in blake bottle can be monitored in real time, and probe is determined
Data by data wire real-time Transmission into external computer；The interface of sensing probe and data wire is fixed on bottle cap, and really
Protect and be fully sealed between probe and bottle cap and bottle cap and blake bottle；Blake bottle bottleneck is changed to italic mouthful and rotation is fixed on
, so can conveniently sample-adding, sampling and washing on support.
Brief description of the drawings
Fig. 1 culture systems structural representations of the present invention；
Fig. 2 present invention culture sub-unit structure schematic diagrames；
Fig. 3 lighting simulation control unit structural representations.
In figure：1- cultivates bottleneck；2- light shields；3- water inlets；4- filters；5- recirculated water baths；The black blake bottles of 7-；8-
Dissolved oxygen sensing probe；9- supports；10- optic panels；11- delivery ports；12- water-bath covers；The white blake bottles of 13-；14- data wires；15-
Center rotational shaft；16- blake bottle runing rests；17- light quantum meters；18- computers；19- fluorescent tubes；20- current controllers；21-
Timer；22- power supplys.
Arrow represents water (flow) direction in Fig. 2.
Embodiment
The present invention is described in further details below in conjunction with the accompanying drawings.
Utilization light and dark bottle technique as shown in Figure 1-2 determines the simulation in-situ batch culture system of primary productivity of marine ecosystem, including training
Support unit, temperature and water flow simulation control unit, lighting simulation control unit.
Cultivating unit includes 5 culture subelements, and each culture subelement includes the water-bath cover 12 of cylindrical shape, in circle
Cylinder along center rotational shaft 15 is set at axis, the periphery of center rotational shaft 15 sets blake bottle runing rest 16, blake bottle rotation
It is removably secured 6 blake bottles, including 3 white blake bottles 13 and 3 black blake bottles 7 on support 16, white blake bottle 13 and black
Blake bottle 7 is intervally arranged, and is symmetrical arranged with center rotational shaft 15 for symmetry axis.All blake bottles are that angle is set, and culture
Bottleneck 1 is arranged on the outside of water-bath cover 12.Water inlet 3 and delivery port 11 are set on water-bath cover 12, and water inlet 3 is arranged on cylinder
On the side of shape water-bath cover 12 and the rotational trajectory just to blake bottle, delivery port 11 is arranged on the side relative with water inlet 3.
Each culture subelement is arranged in series with center rotational shaft 15 for axis, there is gap between each culture subelement, thus
The operation to each culture subelement is not interfered with.
A dissolved oxygen sensing probe 8 is placed in each blake bottle, dissolved oxygen sensing probe 8 is arranged in blake bottle
Heart position, can the real time measure dissolved oxygen micro change；Sensing probe 8 connects data wire, and all data wires 14 are integrated in
In hollow center rotational shaft 15, lean out and data wire 14 to be connected into computer 18 in bottom by center rotational shaft 15.
Water-bath cover 12 is made of high-transmittance plastics, does not interfere with the injection of illumination, and light weight is non-friable, conveniently removes
Fortune.
Temperature and water flow simulation control unit include recirculated water bath 5, due to temperature between each water layer in the present embodiment and
Flow dynamic is more or less the same, therefore controls temperature and water with same recirculated water bath 5 by the way of water-bath cover 12 is connected
Stream.Recirculated water bath 5 is connected with the water inlet 3 of first water-bath cover 12, the delivery port 11 of first water-bath cover 12 and second
The water inlet 3 of water-bath cover 12 is connected, and the delivery port 11 of second water-bath cover 12 is connected with the water inlet 3 of the 3rd water-bath cover 12,
The delivery port 11 of 3rd water-bath cover 12 is connected with the water inlet 3 of the 4th water-bath cover 12, the delivery port of the 4th water-bath cover 12
11 are connected with the water inlet 3 of the 5th water-bath cover 12, and the delivery port 11 of the 5th water-bath cover 12 is connected with recirculated water bath 5.Control
Warm process is as follows：The thermostatted water stream flowed out from recirculated water bath 5 enters first by the water inlet 3 of first water-bath cover 12 and cultivated
Subelement, and follow-up cultivation subelement is sequentially entered, constant temperature current are rotated in each interior circulation of water-bath cover 12, finally from the 5th water
The outflow of delivery port 11 of steam-inflated plastic shroud returns to recirculated water bath 5.Constant temperature current are while temperature control, and driving blake bottle is rotated, blake bottle
And then blake bottle runing rest 16 is driven, so that all blake bottles and runing rest 16 surround center rotational shaft 15 tubular
The interior circulation of transparent water steam-inflated plastic shroud 12 is rotated.By controlling the speed of injection current, to control blake bottle velocity of rotation；So as to mould
Intend the disturbance and influence of physics hydrodynamic condition and wave, current on phytoplankton ecology environment.
Lighting simulation control unit as shown in Figure 3 is arranged in the outside of outermost culture subelement, including light
Panel 10, fluorescent tube 19, fluorescent tube 19 are arranged on optic panel 10, fluorescent tube 19, current controller 20, timer 21
It is sequentially connected with power supply 22.
Optic panel 10 is parallel with the bottom surface of cylindrical water-bath cover 12, makes to be located at each blake bottle in same culture subelement and connects
The intensity of illumination being subject to is identical.
Light quantum meter 17 is set towards the side of lighting simulation control unit in each water-bath cover 12, light quantum meter 17 is close to
Water-bath cover 12, and positioned at by the center of circle of the bottom surface center of circle of cylindrical water-bath cover 12, the center of circle to blake bottle middle part distance for partly
On the circumference of the circle in footpath.Light quantum meter 17 is connected with computer, for detecting luminous intensity at any time, to ensure that Optical power values are accurate.
Between each two culture subelement, a filter 4 is set, and filter 4 is using mist blue material (Mist
), Blue model Lee Filters No.061；Filter 4 is used for the intensity of illumination for controlling to be irradiated to different water levels blake bottle,
With reference to measure of the light quantum meter 17 to light intensity, make the light intensity that is irradiated on blake bottle and raw water layer completely the same.
Whole culture unit is fixed on support 9, is employed in the present embodiment and is longitudinally set the culture subelement of each series connection
The mode put.
This culture systems is arranged in addition to recirculated water bath in lighttight light shield 2.To avoid natural light outside line
Interference, used using a side of totally enclosed lighttight outside light shield 2, and light shield 2 light tight and closed
Drawing curtain, so both can have been played in incubation closing illumination effect, can also draw the curtain apart to be taken after incubation
Sample.Outside light shield 2 uses rigid, when so rocking or move on scientific surveying ship, can protect the transparent of the inside
Water-bath cover 12.The body of light shield 2 is tetragonal body, therefore conveniently cumulative can be stacked；Meanwhile, if for diverse geographic location
The water sample that is obtained of sampling website cultivated, can be stacked together with this multiple kind equipment, and then save on research ship
Shared space.Recirculated water bath is placed on the reason for light shield 2 is outer and is：One is the space for saving light shield 2, and two are placed on
Outside is easily radiated, and three be convenient operation.
Claims (6)
1. a kind of utilization light and dark bottle technique determines the simulation in-situ batch culture system of primary productivity of marine ecosystem, it is characterised in that including training
Support unit, temperature and water flow simulation control unit, lighting simulation control unit；Cultivating unit includes several culture subelements,
The culture subelement includes the water-bath cover of cylindrical shape（12）, center rotational shaft is set along axis in cylinder
（15）, center rotational shaft（15）Periphery sets blake bottle runing rest（16）, blake bottle runing rest（16）On be removably secured
Several blake bottles, each culture subelement is with center rotational shaft（15）It is arranged in series, is deposited between each culture subelement for axis
In gap；Temperature and the water flow simulation control unit includes recirculated water bath（5）, recirculated water bath（5）With water-bath cover（12）
On water inlet（3）And delivery port（11）Connect respectively；Lighting simulation control unit includes optic panel（10）, fluorescent tube
（19）, fluorescent tube（19）It is arranged on optic panel（10）On, fluorescent tube（19）Connect power supply（22）, lighting simulation control unit
It is arranged in the outside of outermost culture subelement；
In each water-bath cover（12）Towards the side of lighting simulation control unit, light quantum meter is set（17）, light quantum meter（17）Tightly
Agio steam-inflated plastic shroud（12）, and positioned at cylindrical water-bath cover（12）The bottom surface center of circle be the center of circle, the center of circle to blake bottle middle part away from
From on the circumference for the circle of radius, light quantum meter（17）It is connected with computer；
Between the culture subelement, a filter is set（4）；
The culture systems remove recirculated water bath（5）Lighttight light shield is arranged at outside（2）It is interior, and light shield（2）'s
One side uses light tight and closed drawing curtain, light shield（2）Using rigid, body is tetragonal body；
The blake bottle is set for angle, cultivates bottleneck（1）It is arranged on water-bath cover（12）Outside and be fixed on blake bottle rotation
Support（16）On.
2. utilization light and dark bottle technique according to claim 1 determines the simulation in-situ batch culture system of primary productivity of marine ecosystem, its
It is characterised by, the quantity of blake bottle is 6 in each culture subelement, including 3 white blake bottles（13）With 3 black cultures
Bottle（7）, white blake bottle（13）With black blake bottle（7）It is intervally arranged, with center rotational shaft between blake bottle（15）For symmetrical axial symmetry
Set.
3. utilization light and dark bottle technique according to claim 1 or 2 determines the simulation in-situ batch culture system of primary productivity of marine ecosystem,
Characterized in that, placing a dissolved oxygen sensing probe in each blake bottle（8）, dissolved oxygen sensing probe（8）It is arranged on
The center of blake bottle；Dissolved oxygen sensing probe（8）Connect data wire（14）, and by all data wires（14）It is integrated in hollow
Center rotational shaft（15）It is interior, pass through center rotational shaft（15）Lean out and in bottom, and by data wire（14）It is connected to dissolved oxygen detection
Device or computer（18）.
4. utilization light and dark bottle technique according to claim 1 or 2 determines the simulation in-situ batch culture system of primary productivity of marine ecosystem,
Characterized in that, the water inlet（3）With delivery port（11）It is arranged on cylindrical water-bath cover（12）Side on, just to culture
The rotational trajectory of bottle.
5. utilization light and dark bottle technique according to claim 1 or 2 determines the simulation in-situ batch culture system of primary productivity of marine ecosystem,
Characterized in that, the optic panel（10）With cylindrical water-bath cover（12）Bottom surface it is parallel.
6. utilization light and dark bottle technique according to claim 1 or 2 determines the simulation in-situ batch culture system of primary productivity of marine ecosystem,
Characterized in that, the fluorescent tube（19）And power supply（22）Between also set up current controller（20）And/or timer（21）.
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