CN105176985A - SSR molecular marker primer of distant hybridization wheat, application and screening method - Google Patents
SSR molecular marker primer of distant hybridization wheat, application and screening method Download PDFInfo
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Abstract
The invention provides an SSR molecular marker primer of distant hybridization wheat, an application and a screening method. The primer comprises Xssrp19, Xssrp81, Xssrp91, Xssrp97, Xssrp138, Xssrp157 and Xssrp201. The SSR molecular marker primer of the distant hybridization wheat is used for searching wheat varieties obtained after distant hybridization for gibberellic disease antigens.
Description
Technical field
The present invention relates to wheat genetic breeding field, particularly relate to SSR molecular marker primer of a kind of distant hybirdization wheat and uses thereof and screening method.
Background technology
Wheat scab (FHB) is mainly caused by wheat infection Fusarium graminearum.It mostly occurs in warm damp and hot area.Due to the global warming that atmospheric pollution nearly ten years brings, the injured area of wheat scab expands year by year, and wheat yield is reduced.In China, wheat is as the staple food crop being only second to paddy rice, and cultivated area over the years is the 22-30% of national total cultivated area and the 20-27% of the food crop total area, distributes throughout national each province (city, district).Wheat scab China middle and lower reach of Yangtze River and Winter Wheat Area, south China and eastern part of Northeast spring wheat district particularly serious, in recent years also occasionally have generation in the Huanghe valley and other area.In recent years, wheat scab has the trend of north Mai Qu expansion at home, and China has the province of 2/3rds that head blight occurs, and during severe epidemic, injured area is more than 7,000,000 hectares.
Cultivating scab resistance kind is one of the most effective means of preventing and treating wheat scab harm at present.The factor causing wheat scab to occur to develop is a lot.The difference of environment and pathogenic bacteria, all can have influence on the evaluation result to scab resistance.As run into weather short of rain, field test is adopted just likely to there will be false disease resistance.And the cycle of this method is longer, does not allow the repetitive identified that is easy to do.So just need other method to assist simultaneously.Toxin identification method just supplements the defect of field test.Its result can also be given counterevidence the result of field test.
Because wheat scab resistance has typical quantitative character feature, by controlled by multiple genes, easily affected by environment, early generation is difficult to select it.In addition, the economical character of much anti gibberellic disease kind matter is poor, and regional adaptability is comparatively strong, and utilizing traditional breeding way to cultivate resistant variety wastes time and energy and also may can not get expected result, molecule marker and assisted selection thereof, for wheat breeding provides an effective approach.Utilize molecule marker, can directly select the genotype of strain.It can weaken the impact of envrionment conditions on result greatly.And its cycle is short, can after planting a couple of days be detected seedling.This will save greatly, cultivate manpower, financial resources and the material resources of wasting in plant.
Utilize DNA molecular marker technology, the site of its Resistance QTL can be verified preferably, carry out the assisted selection of wheat anti gibberellic disease.Utilize molecular markers for identification, there is feature accurately and rapidly.Combine the wheat breed excellent with Comprehensive Traits by the assisted Selection of objective trait related molecular marker to backcross, be expected to break the bad chain of proterties, thus cultivate anti gibberellic disease harvesting high-quality high-yield new variety of wheat.SSR and DArT mark has the advantages that to enrich heritable variation, the molecule marker of widespread use in the gene mapping and association analysis of wheat.Wherein, SSR marker is the special primer of the relative conserved sequence design according to satellite two ends micro-in genome, and pcr amplification is stablized, and detection technique is simple and ripe, is more suitable for molecular marker gene location and assistant breeding selection
But in prior art, the good kind of anti gibberellic disease, economical character is not good.And due to the impact of bad gene linkage, directly with the mixing breed that itself and yielding ability are good, be difficult to obtain that desirable anti gibberellic disease is got bumper crops again simultaneously, good quality wheat new variety.Therefore, create the anti-source of new head blight, Wheat Breeding For Scab Resistance tool is had very important significance.Scientists is screened near isogenic wheat line, but never breakthrough.
Summary of the invention
The object of this invention is to provide and a kind ofly can identify SSR molecular marker primer of distant hybirdization wheat scab disease resistance genes involved and uses thereof and screening method.
In order to solve the problems of the technologies described above, SSR molecular marker primer of distant hybirdization wheat provided by the invention and uses thereof is achieved in that with screening method
A kind of SSR molecular marker primer of distant hybirdization wheat, described primer comprises Xssrp19, Xssrp81, Xssrp91, Xssrp97, Xssrp138, Xssrp157 and Xssrp201, and the sequence corresponding to each primer is: Xssrp19: forward ACATCATCAACTATGCCGAACA
Reverse CGCCTTTAGATCCCACCC
Xssrp81: forward CGAGGGTCAAACGCAAAG
Reverse ATCTCCGACGACGAGGGTG
Xssrp91: forward CTACTTTGCCGTGTTCTTCTC
Reverse GCCGTGGACTTCCATTTCT
Xssrp97: forward CTGAAAGGAGCAACATAT
Reverse TAAACAACCAAGGAAGAA
Xssrp138: forward GGAGCGGAATGGATAAATAA
Reverse GGAAGGAAACGCAGGAGA
Xssrp157: forward GTCGTGCTTGCGTTAGAT
Reverse GACTTGGAATGGAGGTTG
Xssrp201: forward TTGCCACGAAGTGATTGC
Reverse TTGGATGATGCCCTGACC.
Optionally, the annealing temperature of described primer is respectively:
Xssrp19：55℃
Xssrp81：60℃
Xssrp91：50℃
Xssrp97：55℃
Xssrp138：50℃
Xssrp157：55℃
Xssrp201：55℃。
Optionally, the edge kind far away of wheat is triticale.
Optionally, Xssrp19, Xssrp81, Xssrp138 are positioned on karyomit(e) 7A, and Xssrp91, Xssrp97, Xssrp157 and Xssrp138 are positioned on karyomit(e) 3BS.
The screening method of SSR molecular marker primer, comprising:
A, from wheat edge species far away, determine head blight height sense kind and head blight high resistance kind;
B, by wheat scab height sense kind and wheat scab high resistance kind polymorphism preliminary screening is carried out to 201 pairs of SSR molecular marker primers;
C, by the RIL strain of wheat scab high resistance kind, common wheat and above-mentioned high resistance kind and common wheat, polymorphism is carried out to the SSR molecular marker primer that preliminary screening goes out and sieve again, Molecular Markers Linkage Map structure is carried out to the SSR molecular marker primer sifted out again.
Optionally, described wheat edge far away species comprise triticale, little lay down wheat and little Lai wheat.
Optionally, described steps A comprises: during (1) wheat flower, adopts single flower inoculation method to carry out head blight inoculated identification, 21d after inoculation, the sick spikelet number of investigation inoculation fringe; The disease index of each kind is calculated with EXCEL; The single flower inoculation inoculation grading standard formulated with reference to academy of agricultural sciences of Jiangsu Province carries out grade classification; (2) indoor Toxin identification is carried out to wheat simultaneously, use gibberella Raw toxin process wheat seedling and calculate the long inhibiting rate of root, the long inhibiting rate of bud and cell injury degree, detect the resistance of different varieties wheat for gibberella Raw toxin; (3) between basis, the qualification of single flower inoculation scab resistance and indoor Toxin identification result carry out systematic comparison and correlation statistics analysis, determine head blight high resistance kind and head blight height sense kind;
Described step B comprises: the DNA extracting head blight high resistance kind and each product grow wheat of head blight height sense kind; Carry out pcr amplification with the DNA of 201 pairs of above-mentioned each product grow wheats of SSR molecular marker primer pair respectively, then carry out electrophoretic analysis, preliminary screening goes out to have the SSR molecular marker primer of polymorphism;
Described step C comprises: the DNA of each product grow wheat of RIL strain extracting wheat scab high resistance kind, common wheat and above-mentioned high resistance kind and common wheat; The DNA of the above-mentioned each product grow wheat of SSR molecular marker primer pair gone out with step B examination respectively carries out pcr amplification, then carries out electrophoretic analysis, sifts out the SSR molecular marker primer with polymorphism again, its Molecular Markers Linkage Map of application Joinmap4.0 software building.
Optionally, described PCR reaction system is: 25ul, wherein 10Xbuffer2.5ul, 25mMMg2+1.5ul, 2.5mMdNTPs0.2ul, forward primer and each 1ul of reverse primer, Taq enzyme (5U/ul) 0.2ul, template DNA 2.5ul, and moisturizing is to 25ul;
Described pcr amplification reaction parameter is after 94 DEG C of denaturation 5min, 94 DEG C of sex change 1min, 50 ~ 60 DEG C of annealing 1min, and 72 DEG C extend 1min, circulate 34 times, and last 72 DEG C extend 8min, preserve at 4 DEG C;
Pcr amplification product carries out electrophoretic separation on 8% non-denaturing polyacrylamide gel, and after electrophoresis completes, gel uses distilled water wash 2 times after soaking concussion 10min with 0.2% silver nitrate solution, then takes a picture on ultraviolet transilluminator.
Optionally, described pcr amplification use instrument is the T1 type PCR amplification instrument in Biometra company.
SSR molecular marker primer can be used for the genes involved identifying distant hybirdization wheat scab resistance.
7 pairs of SSR primers provided by the invention, are tentatively incorporated on the molecular genetic linkage map delivered respectively, can increase existing molecule marker density, improve genetic map density, are conducive to Wheat Breeding For Scab Resistance.
Accompanying drawing explanation
Fig. 1 is the pcr amplification figure of P138 provided by the invention as primer;
Fig. 2 is the pcr amplification figure of P187 provided by the invention as primer;
Fig. 3 be in 7 pairs of SSR primers provided by the invention Xssrp91 as the pcr amplification figure of primer;
Fig. 4 be in 7 pairs of SSR primers provided by the invention Xssrp201 as the pcr amplification figure of primer;
Fig. 5 is the chromosomal genetic map linkage group of wheat 3BS that the present invention builds;
Fig. 6 is the chromosomal genetic map linkage group of wheat 7A that the present invention builds.
Embodiment
Clearly understand that in order to make object of the present invention, technical scheme and advantage following examples are further elaborated to the present invention.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
A kind of SSR molecular marker primer sequence for the identification of distant hybirdization wheat scab resistance genes involved provided by the invention, described primer sequence sequence as shown in Xssrp19, Xssrp81, Xssrp91, Xssrp97, Xssrp138, Xssrp157 and Xssrp201 in table two.
The screening method of the above-mentioned SSR molecular marker primer for the identification of distant hybirdization wheat scab resistance genes involved comprises the steps:
Step 101, from wheat edge species far away, determine head blight height sense kind and wheat scab high resistance kind; The wheat edge species far away that the present embodiment adopts are alien addition lines triticale 7 kinds, little wheat 5 kinds and little Lai wheat 6 kinds (specifically naming in table 1) of laying down.
This step realizes in the following way: the qualification of (one) each kind wheat scab resistance above-mentioned:
(1) during wheat flower, single flower inoculation method is adopted to carry out head blight inoculated identification, 21d after inoculation, the sick spikelet number of investigation inoculation fringe.The disease index of each kind is calculated with EXCEL.The single flower inoculation inoculation grading standard formulated with reference to academy of agricultural sciences of Jiangsu Province carries out grade classification;
(2) indoor Toxin identification is carried out to wheat simultaneously, use gibberella Raw toxin process wheat seedling and calculate the long inhibiting rate of root, the long inhibiting rate of bud and cell injury degree, detect the resistance of different varieties wheat for gibberella Raw toxin;
(3) between basis, the qualification of single flower inoculation scab resistance and indoor Toxin identification result carry out systematic comparison and correlation statistics analysis.Therefrom select high resistance pond, high sense pond is to carry out next step invention.
Result shows, little wheat type of laying down, triticale type, little Lai wheat type this three interracial anti gibberellic diseases are capable there is significant difference: the wheat breed of triticale type substantially all belongs to resistant variety (R type), just there is high resistance and anti-difference; The wheat breed of little Lai wheat type substantially all belongs to susceptible kind (S type), just high sense and the difference felt; And the wheat breed resistance of little wheat type of laying down is placed in the middle.Illustrate that rye is the better anti-source of head blight, can build group with common wheat is structure for elementary Molecular Markers Linkage Map.As shown in Table 1, the code table of wheat breed and establishment, therefrom selects these 3 kind composition high resistance ponds of B1, B3, B5, these 3 kind composition high sense ponds of C1, C5, C6.
The code table of table 1 wheat breed and establishment
Step 102, extraction wheat scab height sense kind and wheat scab high resistance kind DNA;
The DNA of high resistance kind (B1, B3, B5), high sense kind (C1, C5, C6) each product grow wheat is extracted respectively by CTAB method.
Step 103, with described wheat scab height sense kind and wheat scab high resistance kind DNA for template, it carries out pcr amplification to use 201 pairs of SSR primer pairs respectively;
The 201 pairs of SSR primers are by reference to carrying out the relevant report of SSR molecular marker to wheat scab resistance and application primer-design software PrimerPremier5.0 designs in conjunction with GenBank database information.
PCR reaction system is 25ul, wherein 10Xbuffer2.5ul, 25mMMg2+1.5ul, 2.5mMdNTPs0.2ul, forward primer and each 1ul of reverse primer, Taq enzyme (5U/ul) 0.2ul, template DNA 2.5ul, and moisturizing is to 25ul;
Pcr amplification parameter is after 94 DEG C of denaturation 5min, 94 DEG C of sex change 1min, 60 DEG C of annealing 1min, and 72 DEG C extend 1min, circulate 34 times, and last 72 DEG C extend 8min, preserve at 4 DEG C; In the enterprising performing PCR amplification of the T1 type PCR amplification instrument of Biometra company.
Step 104, pcr amplification product carry out electrophoretic analysis
Pcr amplification product carries out electrophoretic separation on 8% non-denaturing polyacrylamide gel, after electrophoresis completes, gel uses distilled water wash 2 times after soaking concussion 10min with 0.2% silver nitrate solution, then takes a picture on ultraviolet transilluminator, filters out 28 pairs of SSR primers with polymorphism.As Fig. 1, shown in Fig. 2, Fig. 1 is the electrophorogram after primer P138PCR increases, label is 1, 2, 3 represent high resistance kind B1 respectively, B3, B5 is the pcr amplification result of template, label is 4, 5, 6 represent high sense kind C1 respectively, C5, C6 is the pcr amplification result of template, as we can see from the figure, with high resistance kind B1, B3, B5 is template, amplify product, and with height sense kind C1, C5, C6 is template, do not amplify the product with high resistance kind amplified production same molecular amount, illustrate that primer P138 has polymorphism, in 28 pairs of primers, other 27 pairs of primer results are identical with Fig. 1.Fig. 2 is the electrophorogram after primer P187PCR increases, label is the pcr amplification result that 1,2,3 represent high resistance kind B1 respectively, B3, B5 are template, label is the pcr amplification result that 4,5,6 represent high sense kind C1 respectively, C5, C6 are template, as we can see from the figure, no matter with high resistance kind B1, B3, B5 for template, or with height sense kind C1, C5, C6 for template, all there is the product amplifying same molecular amount, illustrate that primer P187 does not have polymorphism.
The 28 pairs of SSR primers with polymorphism filtered out are as shown in Table 2:
Table 2 has 28 pairs of SSR primer sequence tables of polymorphism
Step 105, with filter out 28 pairs of SSR primers, with common wheat and wheat scab high resistance kind RIL strain DNA for template carries out pcr amplification;
Rye/Henan wheat No. 2 hybridization builds RIL strain, has 28 of polymorphism pairs of SSR primer pairs 133 RIL strain DNA to carry out multiple sieve.
PCR reaction system is 25ul, wherein 10Xbuffer2.5ul, 25mMMg2+1.5ul, 2.5mMdNTPs0.2ul, forward primer and each 1ul of reverse primer, Taq enzyme (5U/ul) 0.2ul, template DNA 2.5ul, and moisturizing is to 25ul;
Pcr amplification parameter is after 94 DEG C of denaturation 5min, 94 DEG C of sex change 1min, 60 DEG C of annealing 1min, and 72 DEG C extend 1min, circulate 34 times, and last 72 DEG C extend 8min, preserve at 4 DEG C; In the enterprising performing PCR amplification of the T1 type PCR amplification instrument of Biometra company.
Step 106, pcr amplification product carry out electrophoretic analysis;
Pcr amplification product carries out electrophoretic separation on 8% non-denaturing polyacrylamide gel, and after electrophoresis completes, gel uses distilled water wash 2 times after soaking concussion 10min with 0.2% silver nitrate solution, then takes a picture on ultraviolet transilluminator.According to electrophoresis result, the amplification of reference wheat scab high resistance kind and common wheat, the amplification filtering out RIL strain presents the SSR molecular marker primer of polymorphism, have 7 pairs of primers, Xssrp19, Xssrp81, Xssrp91, Xssrp97, Xssrp138, Xssrp157 and Xssrp201 respectively, as shown in Figure 3 and Figure 4, in figure, 1 is high resistance (female parent), 2 is high sense (male parent), 3 ~ 13 is random RIL system, 7 pairs of SSR primers of screening as shown in Table 3:
7 pairs of SSR primer sequence tables that table 3 is mapped for molecule chain lock
Step 107, application Joinmap4.0 software building genetic map.
Above-mentioned 7 primers, the molecular genetic linkage map on the 3BS wherein having 4 to be tentatively incorporated into respectively to have delivered, 3 are tentatively incorporated on 7A, as shown in Figure 5 and Figure 6.
Utilize Zhouetal and Shenetal to deliver be positioned SSR marker Xgwm389 on 3BS, Xgwm493, Xgwm533, XBarc147, XBarc131, XBarc238, XBarc217.3, XCS-SSR7, XCS-SSR20 carry out gene type in colony, sets up the elementary linkage map of 3BS scab resistance QTL region based on SSR marker.The present invention adds 4 SSR marker on 3BS, respectively called after Xssrp91, Xssrp97, Xssrp201 and Xssrp157.As shown in Figure 5, Xssrp201 is positioned at 2.6cM place outside Xbrc131, Xssrp91 is positioned at 5.7cM place of 2.6cM place outside Xbrc131, Xssrp97 is positioned at the spacing XCS-SSR73.0cM of Xssrp91 and XCS-SSR7, the position of Xssrp157 close Xgwm4930.7cM between Xgwm493 and Xbarc147.
The series monomer that Nicho1son (2000) utilizes anti gibberellic disease kind Tritieummaeha to be chromosomal donor, located B1,4A relevant with anti-red gene with 7A karyomit(e).The present invention adds 3 SSR marker called after Xssrp19, Xssrp81, Xssrp138 respectively on 7A karyomit(e).As shown in Figure 6, Xssrp81 is 0.2cM place outside Xwmc168-7A, and Xssrp138 is 0.2cM place inside Xwmc479, and Xssrp19 is at the spacing Xwmc479-7A0.6Cm of Xssrp138 and Xwmc479-7A.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a SSR molecular marker primer for distant hybirdization wheat, is characterized in that, described primer comprises Xssrp19, Xssrp81, Xssrp91, Xssrp97, Xssrp138, Xssrp157 and Xssrp201, and the sequence corresponding to each primer is:
Xssrp19: forward ACATCATCAACTATGCCGAACA
Reverse CGCCTTTAGATCCCACCC
Xssrp81: forward CGAGGGTCAAACGCAAAG
Reverse ATCTCCGACGACGAGGGTG
Xssrp91: forward CTACTTTGCCGTGTTCTTCTC
Reverse GCCGTGGACTTCCATTTCT
Xssrp97: forward CTGAAAGGAGCAACATAT
Reverse TAAACAACCAAGGAAGAA
Xssrp138: forward GGAGCGGAATGGATAAATAA
Reverse GGAAGGAAACGCAGGAGA
Xssrp157: forward GTCGTGCTTGCGTTAGAT
Reverse GACTTGGAATGGAGGTTG
Xssrp201: forward TTGCCACGAAGTGATTGC
Reverse TTGGATGATGCCCTGACC.
2. SSR molecular marker primer according to claim 1, is characterized in that, the annealing temperature of described primer is respectively:
Xssrp19：55℃
Xssrp81：60℃
Xssrp91：50℃
Xssrp97：55℃
Xssrp138：50℃
Xssrp157：55℃
Xssrp201：55℃。
3. SSR molecular marker primer according to claim 1 and 2, is characterized in that, the edge kind far away of wheat is triticale.
4. SSR molecular marker primer according to claim 3, is characterized in that, Xssrp19, Xssrp81, Xssrp138 are positioned on karyomit(e) 7A, and Xssrp91, Xssrp97, Xssrp157 and Xssrp138 are positioned on karyomit(e) 3BS.
5. the screening method of SSR molecular marker primer according to claim 1, is characterized in that, comprising:
A, from wheat edge species far away, determine head blight height sense kind and head blight high resistance kind;
B, by wheat scab height sense kind and wheat scab high resistance kind polymorphism preliminary screening is carried out to 201 pairs of SSR molecular marker primers;
C, by the RIL strain of wheat scab high resistance kind, common wheat and above-mentioned high resistance kind and common wheat, polymorphism is carried out to the SSR molecular marker primer that preliminary screening goes out and sieve again, Molecular Markers Linkage Map structure is carried out to the SSR molecular marker primer sifted out again.
6. the screening method of SSR molecular marker primer according to claim 5, is characterized in that, described steps A comprises: during (1) wheat flower, adopts single flower inoculation method to carry out head blight inoculated identification, 21d after inoculation, the sick spikelet number of investigation inoculation fringe; The disease index of each kind is calculated with EXCEL; The single flower inoculation inoculation grading standard formulated with reference to academy of agricultural sciences of Jiangsu Province carries out grade classification; (2) indoor Toxin identification is carried out to wheat simultaneously, use gibberella Raw toxin process wheat seedling and calculate the long inhibiting rate of root, the long inhibiting rate of bud and cell injury degree, detect the resistance of different varieties wheat for gibberella Raw toxin; (3) between basis, the qualification of single flower inoculation scab resistance and indoor Toxin identification result carry out systematic comparison and correlation statistics analysis, determine head blight high resistance kind and head blight height sense kind;
Described step B comprises: the DNA extracting head blight high resistance kind and each product grow wheat of head blight height sense kind; Carry out pcr amplification with the DNA of 201 pairs of above-mentioned each product grow wheats of SSR molecular marker primer pair respectively, then carry out electrophoretic analysis, preliminary screening goes out to have the SSR molecular marker primer of polymorphism;
Described step C comprises: the DNA of each product grow wheat of RIL strain extracting wheat scab high resistance kind, common wheat and above-mentioned high resistance kind and common wheat; The DNA of the above-mentioned each product grow wheat of SSR molecular marker primer pair gone out with step B examination respectively carries out pcr amplification, then carries out electrophoretic analysis, sifts out the SSR molecular marker primer with polymorphism again, its Molecular Markers Linkage Map of application Joinmap4.0 software building.
7. the screening method of SSR molecular marker primer according to claim 6, it is characterized in that, described PCR reaction system is: 25ul, wherein 10Xbuffer2.5ul, 25mMMg2+1.5ul, 2.5mMdNTPs0.2ul, forward primer and each 1ul of reverse primer, Taq enzyme (5U/ul) 0.2ul, template DNA 2.5ul, moisturizing is to 25ul; Described pcr amplification reaction parameter is after 94 DEG C of denaturation 5min, 94 DEG C of sex change 1min, 50 ~ 60 DEG C of annealing 1min, and 72 DEG C extend 1min, circulate 34 times, and last 72 DEG C extend 8min, preserve at 4 DEG C; Pcr amplification product carries out electrophoretic separation on 8% non-denaturing polyacrylamide gel, and after electrophoresis completes, gel uses distilled water wash 2 times after soaking concussion 10min with 0.2% silver nitrate solution, then takes a picture on ultraviolet transilluminator.
8. the screening method of SSR molecular marker primer according to claim 7, is characterized in that, described wheat edge far away species comprise triticale, little lay down wheat and little Lai wheat.
9. the screening method of SSR molecular marker primer according to claim 8, is characterized in that, described pcr amplification use instrument is the T1 type PCR amplification instrument in Biometra company.
10. SSR molecular marker primer according to claim 1 can be used for the genes involved identifying distant hybirdization wheat scab resistance.
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| CN107868846A (en) * | 2017-12-21 | 2018-04-03 | 山西省林业科学研究院 | The SSR molecular marker primer of oak category and the method and its application for identifying nearly edge oak category kind |
| CN112725524A (en) * | 2021-03-15 | 2021-04-30 | 山东省农业科学院作物研究所 | General markers for detecting wheat closely related species and application thereof |
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| CN112695125B (en) * | 2021-01-29 | 2021-08-27 | 广东省农业科学院环境园艺研究所 | Katelia SSR molecular marker primer composition and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1442039A (en) * | 2003-01-28 | 2003-09-17 | 江苏省农业科学院 | Molecular label closely linked with wheat gibberellin resistance main effect QTL and its application |
| CN101760537A (en) * | 2008-12-19 | 2010-06-30 | 李祥 | Application of SSR and EST-SSR mark in wheat |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1459226A (en) * | 2003-01-28 | 2003-12-03 | 江苏省农业科学院 | Wheat gibberella resistance major effect QTL linked molecular label and its application |
| CN1246478C (en) * | 2003-06-23 | 2006-03-22 | 南京农业大学 | Molecule marker method of wheat Wangshuibai scab resistant major gene locus |
| CN101892307A (en) * | 2010-05-12 | 2010-11-24 | 江苏省农业科学院 | SSCP marker closely linked with major wheat scab resistance QTL and application thereof |
| CN103146699B (en) * | 2012-03-27 | 2014-07-02 | 南京农业大学 | Molecular marker MAG7237 of wheat fusarium head blight infection resistant gene Fhb4 and application of molecular marker MAG7237 |
-
2015
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1442039A (en) * | 2003-01-28 | 2003-09-17 | 江苏省农业科学院 | Molecular label closely linked with wheat gibberellin resistance main effect QTL and its application |
| CN101760537A (en) * | 2008-12-19 | 2010-06-30 | 李祥 | Application of SSR and EST-SSR mark in wheat |
Non-Patent Citations (4)
| Title |
|---|
| 万永芳 等: "大麦、黑麦和小黑麦种质资源对赤霉病抗性的鉴定", 《植物保护》 * |
| 张炜: "利用小麦SSR研究其近缘物种的遗传多样性及其微卫星序列的进化", 《中国优秀硕士学位论文全文数据库（电子期刊）农业科技辑》 * |
| 张荣: "小麦抗赤霉病性鉴定及QTL初步定位", 《中国优秀硕士学位论文全文数据库（电子期刊）农业科技辑》 * |
| 陈海梅: "小麦 EST-SSR标记的开发、定位和作图", 《中国优秀硕士学位论文全文数据库（电子期刊）农业科技辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107868846A (en) * | 2017-12-21 | 2018-04-03 | 山西省林业科学研究院 | The SSR molecular marker primer of oak category and the method and its application for identifying nearly edge oak category kind |
| CN107868846B (en) * | 2017-12-21 | 2021-02-02 | 山西省林业科学研究院 | SSR molecular marker primer of quercus, method for identifying closely related quercus varieties and application of SSR molecular marker primer |
| CN112725524A (en) * | 2021-03-15 | 2021-04-30 | 山东省农业科学院作物研究所 | General markers for detecting wheat closely related species and application thereof |
| CN112725524B (en) * | 2021-03-15 | 2022-06-10 | 山东省农业科学院作物研究所 | General markers for detecting wheat closely related species and application thereof |
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| Publication number | Publication date |
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| CN110093435A (en) | 2019-08-06 |
| CN110093435B (en) | 2021-01-05 |
| CN105176985B (en) | 2019-01-15 |
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