CN1459226A - Wheat gibberella resistance major effect QTL linked molecular label and its application - Google Patents

Wheat gibberella resistance major effect QTL linked molecular label and its application Download PDF

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Publication number
CN1459226A
CN1459226A CN 03112779 CN03112779A CN1459226A CN 1459226 A CN1459226 A CN 1459226A CN 03112779 CN03112779 CN 03112779 CN 03112779 A CN03112779 A CN 03112779A CN 1459226 A CN1459226 A CN 1459226A
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wheat
qtl
strain
scab
molecular labeling
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任丽娟
陆维忠
沈晓蓉
周淼平
张旭
马鸿翔
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Jiangsu Academy of Agricultural Sciences
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Jiangsu Academy of Agricultural Sciences
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Abstract

A molecular marker associated with the major QTL of wheat resistance to head blight is disclosed. The SSR labelling technique is used to analyze the 894037/Alondra F4 recombinant selfing population. The existance of major QTL on the chromosome 3B of Ning 894037 is detected, and this can be used to explain 42.8% of head blight resistance. The obtained molecular markers Xgwm 533-130, BARC 133-115 and BARC 075-110 for the major QTL of Ning 894037 can be used to culture the disease-resistant wheat variety with high efficiency.

Description

The molecular labeling of wheat scab resistance main effect QTL linkage and application thereof
Technical field
The present invention relates to wheat breeding and biological technical field, especially select to utilize the molecular labeling of wheat anti gibberellic disease resistance main effect QTL linkage to carry out assistant breeding, to improve the efficient of wheat anti gibberellic disease breeding.
Background technology
(Fusarium graminearum, the wheat scab that Schw) causes is a worldwide disease, has a strong impact on the output of wheat and the grain quality of wheat by Fusarium graminearum.It also is one of key factor of puzzlement China small-scale production.At middle and lower reach of Yangtze River winter wheat district, south China winter wheat district and northeast Mai Qu, injured area surpasses 100,000,000 mu, accounts for 1/4th of the national wheat gross area, along with global warming, and the variation of cropping system and mode, wheat scab morbidity scope is expanded gradually.In Chinese forward Huai-Hai and Huanghe valley expansion.Areas such as middle part, the north and Canadian south in the U.S., wheat scab expansion in recent years is swift and violent.Also be expansion trend Europe, South America, so wheat scab has become world's disease that the whole world is paid close attention to.
In decades, the Chinese and foreign department scholar makes great efforts to be devoted to the genetic analysis and the breeding for disease resistance research of head blight always, but producing little effect, mainly is to have brought very big difficulty because the influence of controlled by multiple genes and environment screens disease-resistant germplasm breeding resistant variety for the application conventional method.The fast development of molecular biotechnology, the discovery of disease-resistant molecular labeling has brought a linear light bright for wheat scab breeding man.In recent years, to the anti-red qtl analysis of Soviet Union wheat No. 3 and derived varieties (being) thereof, study often.Employing such as Waldron and Anderson RFLP (Restriction Fragment LengthPolymorphism) and SSR (Simple Sequence Repeat) equimolecular labeling technique are found an anti-red property main effect QTL (Quantitative Trait Loci), the highest soluble 41.6% phenotypic variation in this site on the 3B galianconism of head blight anti-source Soviet Union wheat No. 3 and derived varieties (being) thereof.Bai and Zhou etc. and Buerstmayr etc. also analyze the different Soviet Unions wheat hereditary segregation population that is of deriving for No. 3, have confirmed that 3B chromosome master imitates the existence of anti-red QTL.This seminar is by the recombinant inbred lines in the anti-source of existing different head blight, make up Soviet Union wheat No. 3, Wangshuibai, 894037 3B chromosomal inheritance linkage maps and also carry out qtl analysis, find different anti-sources 3B the short arm of a chromosome all exist one main imitate anti-red QTL site and with the molecular labeling of this main effect QTL compact linkage.Use the molecular labeling with the anti-red property main effect QTL compact linkage in a certain specific anti-source, can not be subjected to the influence of environmental condition, to the offspring in this specific anti-source and the kind of spreading out (being) thereof early for screening seedling stage, save production cost, improve breeding and efficiency of selection.
Peaceful 894037 (the Jiangsu agricultural journals of wheat line, 1998, being this laboratory p129-134) cultivates the somaclone mutant that obtain with raising wheat No. 3 through young fringe, its anti-red property near or reach the level of anti-source Soviet Union wheat No. 3, and economical character is used by breeding man significantly better than Soviet Union wheat No. 3; The molecular labeling report linked with its main effect QTL do not arranged at present as yet.
Summary of the invention
An object of the present invention is to provide the molecular labeling and the application method in the wheat anti gibberellic disease breeding thereof of wheat anti gibberellic disease resistance main effect QTL, in order to define the molecular labeling linked with 894037 scab resistance main effect QTL, can predict the scab resistance of wheat plant by detecting these molecular labelings, accelerate the selection progress of wheat-resistance to scab, on producing, be used to detect the scab resistance level of wheat breed.
The invention provides the molecular labeling with the scab resistance main effect QTL linkage, obtain by the following method:
A) peaceful 894037 (♀) of wheat scab disease-resistant variety and wheat scab susceptible variety (♂) hybridization obtain hybrid F 1
B) by hybrid F 1Self-pollination obtains F 2A plurality of filial generations pass (SSD) method by simple grain and obtain F 8For RIL;
C) each strain system of RIL is carried out molecular marker analysis, and the genotype of each strain system of RIL is described; Concrete grammar: the genomic DNA that separates each strain system of RIL, adopt the SSR primer to carry out pcr amplification, amplified production is electrophoretic separation on the polyacrylamide gel of 6% (containing 5.7 gram acrylamides and 0.3 gram methene acrylamide in the 100ml polyacrylamide sol solution), after silver dyed, obtaining each strain was genotype;
D), make up the wheat genetic linkage map with the molecular labeling data that obtains based on Mendel and Morgan genetic linkage and law of segregation;
E) measure the scab resistance that each strain of RIL is;
F) scab resistance of each strain system of RIL and the molecular labeling in the wheat genetic linkage map are carried out chain and qtl analysis, qtl analysis adopts MapQTL4.0 to carry out, carry out Kruskal-Wallis (K-W) test earlier, P＜0.005 shows this mark and scab resistance close linkage, may there be QTL, then interval mapping (Interval Mapping) and many QTL mapping (MQM Mapping) analysis are carried out in the interval that may have QTL, with 2.5 is the LOD threshold value, there is a QTL site greater than 2.5 explanations, thus SSR molecular labeling Xgwm533-130 definite and the peaceful 894037 scab resistance main effect QTL linkages in anti-source, BARC133-115, BARC075-110.
The application of the molecular labeling that above-mentioned wheat scab resistance QTL is chain, comprise: whether the genotype detection with molecular labeling Xgwm533-130, BARC133-115, BARC075-110 are used for wheat breed or strain has scab resistance to judge certain kind or strain:
A) by with peaceful 894037 and derived varieties (being) be male parent or maternal and other wheat hybridizings and multiply to F 2More than generation;
B) separate the genomic DNA of single wheat plant, detect each wheat plant and whether exist with the head blight master and imitate the linked molecular labeling of resistance QTL; Occur simultaneously if any 2 above molecular labelings, predict that then the head blight of this wheat breed reaches anti-level.
The molecular labeling of wheat anti gibberellic disease main effect QTL of the present invention and the application in the wheat anti gibberellic disease breeding thereof, advantage is: overcome conventional anti gibberellic disease breeding cycle length, inefficient deficiency, the speciality of performance molecular marker assisted selection, develop a kind of efficiently, new technology of breeding fast.For example utilize new varieties of conventional anti gibberellic disease breeding method seed selection or new lines, often need 6-8, in addition the longer time, in per generation, all need colony is carried out the resistance evaluation simultaneously, and workload is very big.In addition, anti gibberellic disease is identified the influence that often is subjected to environmental condition again, and molecular marker assisted selection is not subjected to the influence of environmental condition, just can select scab resistance from generation to generation low, the workload of breeding man be will significantly reduce, accuracy and the breeding efficiency selected improved.
Description of drawings:
Fig. 1 is peaceful 894037 3B chromosomes and qtl analysis LOD value distribution map; The left side is a 3B chromosomal inheritance linkage map, and the map distance between mark marks with data; The curve map on the right is the LOD distribution map of QTL, and dotted line is 2.5 threshold values.
Embodiment: material and method: 1. peaceful 894037/Alondra recombinant inbred lines makes up and the anti-red evaluation of inoculation:
Adopt peaceful 894037 to reach (Alondra) hybridization with wheat sense head blight kind Alon and produce F 1Hybrid, F 1Selfing produces F 2The offspring, F 2The plantation of seed of 219 individual plant picked at random, the method that adopts simple grain to pass is bred F 8In generation, produce 219 familys.
Scab resistance is identified and is all adopted single flower drip-injection method, Fusarium graminearum conidial suspension concentration is 50000/ml, choose the tassel of just having bloomed, drip the 10ul spore suspension in middle part Xiao Hua, the greenhouse atomizing was preserved moisture 3 days, and bagging or artificial atomizing method for moisturizing are then adopted in the field, and each is inoculation 10～12 fringes, in back 21 days of inoculation, investigate disease small ear rate.Sick small ear rate=(morbidity spikelet number/total spikelet number) * 100%
Peaceful 894037/Alondra recombinant inbred lines was released university from sufferings in the U.S. in 2000 respectively with calendar year 2001 and is experimental field carried out the scab resistance evaluation with the greenhouse.2. the chromosomal genetic map of peaceful 894037 3B makes up and qtl analysis:
On peaceful 894037 3B chromosomes, 9 SSR marks are arranged, cover 87.1cM.The K-W test Analysis shows, SSR mark Xgwm389, BAC075, Xgwm533, BAC133, Xgwm493 and the BARC102 that is arranged in the 3B galianconism identifies all and the resistance close linkage at the scab resistance in experimental field and greenhouse, may have scab resistance QTL on this linkage group.Adopt interval mapping analysis, find a QTL site really between BARC075 and BARC102, the LOD value of this QTL considerably beyond the LOD2.5 threshold value, can be explained 28.3%～42.8% anti-red property variation between 12.35～20.11; Composite interval mapping shows that this QTL accurate location is between BARC133 and Xgwm493, and this interval has only 5 cM, the highlyest can explain 37.9% scab resistance.
Embodiment 1
The molecular labeling of wheat scab resistance main effect QTL linkage obtains by the following method:
A) peaceful 894037 (♀) of wheat scab disease-resistant variety and wheat scab susceptible variety Alon reach (Alondra) and (♂) hybridize, and obtain hybrid F 1
B) by hybrid F 1Self-pollination obtains F 2A plurality of filial generations pass (SSD) method by simple grain and obtain F 8For RIL.
C) each strain system of RIL is carried out molecular marker analysis, and the genotype of each strain system of RIL is described; Concrete grammar: the genomic DNA that separates each strain system of RIL, adopt the SSR primer to carry out pcr amplification, amplified production is electrophoretic separation on the polyacrylamide gel of 6% (containing 5.7 gram acrylamides and 0.3 gram methene acrylamide in the 100ml polyacrylamide sol solution), after silver dyed, obtaining each strain was genotype.
D), make up the wheat genetic linkage map with the molecular labeling data that obtains based on Mendel and Morgan genetic linkage and law of segregation.
E) measure the scab resistance that each strain of RIL is.
F) scab resistance of each strain system of RIL and the molecular labeling in the wheat genetic linkage map are carried out chain and qtl analysis, qtl analysis adopts MapQTL4.0 to carry out, carry out Kruskal-Wallis (K-W) test earlier, P＜0.005 shows this mark and scab resistance close linkage, may there be QTL, then interval mapping (Interval Mapping) and many QTL mapping (MQM Mapping) analysis are carried out in the interval that may have QTL, with 2.5 is the LOD threshold value, there is a QTL site greater than 2.5 explanations, thus SSR molecular labeling Xgwm533-130 definite and the peaceful 894037 scab resistance main effect QTL linkages in anti-source, BARC133-115, BARC075-110.
With wheat cdna group DNA, adopt SSR primer BARC075F:5 '-AGGGTTACAGTTTGCTCTTTTAC-3 ', BARC075R:5 '-CCCGACGACCTATCTATACTTCTCTA-3 ', Xgwm533F:5 '-AAGGCGAATCAAACGGAATA-3 ', Xgwm533R:5 '-GTTGCTTTAGGGGAAAAGCC-3 ' and BA RC133F:5 '-AGCGCTCGAAAAGTCAG-3 ', BARC 133R:5 '-GGCAGGTCCAACTC CAG-3 ' carries out pcr amplification, on amplified production (contains 5.7 gram acrylamides and 0.3 gram methylene diacrylamide) 6% in 100ml polyacrylamide sol solution polyacrylamide gel after the electrophoretic separation, obtain three molecular labelings, size is respectively 110bp, 130bp, 115bp.
Embodiment 2
What utilization screened carries out Preliminary detection to the breeding height for material with the linked molecular labeling of peaceful 894037 scab resistance main effect QTLs, to detect the practical value of this method in the wheat scab resistance molecular marker assisted selection.Choose 6 with peaceful 894037 and derived varieties (being) be that male parent or maternal height are for breeding material or wheat breed, separate its genomic DNA, with SSR primer Xgwm533, BARC075 and BARC133 these DNA are carried out the PCR reaction then, behind the electrophoresis according to the size of DNA band or have or not and determine whether to exist corresponding mark, as there being 2-3 mark simultaneously, illustrate that this kind or strain scab resistance reach the level of " resisting ", it then is susceptible not existing, and verifies with scab resistance inoculation qualification result and Markers for Detection result simultaneously.
Subordinate list 3 provides is predicting the outcome and head blight inoculation qualification result of high DNA tests for strain or kind, we can find from table, SSR mark Xgwm533-130, BARC133-115, BARC075-110 and the anti-red qualification result goodness of fit are fine, and above presentation of results molecular marker assisted selection is fully feasible.
Embodiment 3
Obtain with the closely linked molecular labeling of peaceful 894037 scab resistances, also reach the F of (Alondra) hybridization with Alon peaceful 894037 10Offspring's part plant has carried out the scab resistance prediction, the results are shown in subordinate list 4, and it is very accurate to predict.
Now with above each EmbodimentThe analytical test result who relates to concentrates below and lists:
Subordinate list 1:Kruskal-Wallis test result;
The scab resistance qtl analysis result of subordinate list 2:894037/Alondra colony;
Subordinate list 3: use with peaceful 894037 main QTL compact linkage molecule marks and detect with peaceful 894037 and derived varieties
(being) is the scab resistance of the wheat line in anti-source;
Subordinate list 4: use with peaceful 894037 main effect QTL compact linkage molecule marks and predict that peaceful 894037/Alondra's is assorted
Hand over offspring (F 10) scab resistance.Subordinate list 1
Map distance Molecular labeling The K value The P value
????0.00 ????12.7 ????22.5 ????28.0 ????37.4 ????42.6 ??Xgwm389 ??BARC075 ??Xgwm533 ??BARC133 ??Xgwm493 ??BARC102 ????18.3 ????25.6 ????56.9 ????60.8 ????61.8 ????46.0 ????0.0005 ????0.0001 ????0.0001 ????0.0001 ????0.0001 ????0.0001
The K value: Kruskal-Wallis test Analysis value, value is big more show relevant more with scab resistance.
The P value: represent the significance degree relevant with scab resistance, P＜0.005 shows this mark and scab resistance close linkage.
Subordinate list 2
Field greenhouse land for growing field crops/greenhouse between the analytical method mark zone
LOD????VE?????LOD??????VE??????LOD??????VE
Interval mapping BARC075
18.43???40????12.35????28.3????20.11????42.8
-BARC102
Composite interval mapping BARC133
17.77???35.8??12.36????25.3????19.58????37.9
-Xgwm493LOD=logarithm probability value (probability of reflection QTL) VE=% is changed by the phenotype that QTL caused
Subordinate list 3
Kind or combination ?Xgwm533 ?BARC133 ??BARC75 Sick small ear rate (%)
????894037 ????+ ????+ ????+ ????9.6
????Alondra ????- ????- ????- ????20.4
894037/ raises 4 ????+ ????+ ????+ High anti-
Raise 5/894037 ????- ????+ ????+ ????9.5
894037/ west wind 1 ????+ ????+ ????+ ????9.6
????894037/Alondra ????+ ????+ ????+ ????8.9
894037/ west wind 2 ????+ ????+ ????+ ????13.5
Peace farming 8455//894037/ west wind ????+ ????+ ????+ ????13.0
+ expression has the band of SSR mark (Xgwm533-130, BARC133-115, BARC075-110),
-expression does not have the band subordinate list 4 of SSR mark (Xgwm533-130, BARC133-115, BARC075-110)
Family name Predict the outcome Actual result
?Xgwm533 ?BARC133 ?BARC075 Scab resistance Sick small ear rate (%) Scab resistance
??8A004 ????- ????- ????- Sense ????67.2 Sense
??8A006 ????- ????- ????- Sense ????37.9 Sense
??8A008 ????- ????- ????- Sense ????42.1 Sense
??8A009 ????- ????- ????- Sense ????51.2 Sense
??8A012 ????- ????- ????- Sense ????69.4 Sense
??8A016 ????- ????- ????- Sense ????45.7 Sense
??8A025 ????- ????- ????- Sense ????69.0 Sense
??8A026 ????- ????- ????- Sense ????79.0 Sense
??8A027 ????- ????- ????- Sense ????34.8 Sense
??8A032 ????- ????- ????- Sense ????49.8 Sense
??8A042 ????- ????- ????- Sense ????67.7 Sense
??8A044 ????- ????- ????- Sense ????72.2 Sense
??8A019 ????+ ????+ ????+ Anti- ????12.6 Anti-
??8A021 ????+ ????+ ????+ Anti- ????12.0 Anti-
??8A022 ????+ ????+ ????+ Anti- ????18.9 Anti-
??8A038 ????+ ????+ ????+ Anti- ????25.6 Anti-
??8A040 ????+ ????+ ????+ Anti- ????7.5 Anti-
??8A045 ????+ ????+ ????+ Anti- ????10.9 Anti-
??8A051 ????+ ????+ ????+ Anti- ????19.3 Anti-
??8A054 ????+ ????+ ????+ Anti- ????19.1 Anti-
??8A055 ????+ ????+ ????+ Anti- ????14.1 Anti-
??8A077 ????+ ????+ ????+ Anti- ????25.3 Anti-
??8A083 ????+ ????+ ????+ Anti- ????17.1 Anti-
??8A084 ????+ ????+ ????+ Anti- ????9.7 Anti-
+ expression has the band of SSR mark (Xgwm533-130, BARC133-115, BARC075-110),
-expression does not have the band of SSR mark (Xgwm533-130, BARC133-115, BARC075-110).

Claims (6)

1, a kind of molecular labeling of wheat scab resistance main effect QTL linkage is to obtain through the following steps:
A) obtain the hybrid F of peaceful 894037 (♀) of the disease-resistant strain of wheat scab and wheat scab susceptible variety (♂) 1
B) by hybrid F 1Self-pollination obtains F 2A plurality of filial generations pass (SSD) method by simple grain and obtain F 8For RIL;
C) each strain system of RIL is carried out molecular marker analysis, and the genotype of each strain system of RIL is described; Concrete grammar: the genomic DNA that separates each strain system of RIL, adopt the SSR primer to carry out pcr amplification, amplified production is electrophoretic separation on the polyacrylamide gel of 6% (containing 5.7 gram acrylamides and 0.3 gram methylene diacrylamide in the 100ml polyacrylamide sol solution), after silver dyed, obtaining each strain was genotype;
D), make up genetic linkage map with the molecular labeling data that obtains based on Mendel and Morgan genetic linkage and law of segregation;
E) measure the scab resistance that each strain of RIL is;
F) scab resistance of each strain system of RIL and the molecular labeling in the wheat genetic linkage map are carried out chain and qtl analysis, qtl analysis adopts MapQTL4.0 to carry out, carry out Kruskal-Wallis (K-W) test earlier, P＜0.005 shows this mark and scab resistance close linkage, may there be QTL, then interval mapping (Interval Mapping) and many QTL mapping (MQM Mapping) analysis are carried out in the interval that may have QTL, with 2.5 is the LOD threshold value, there is a QTL site greater than 2.5 explanations, thus SSR molecular labeling Xgwm533-130 definite and the peaceful 894037 scab resistance main effect QTL linkages in anti-source, BARC133-115, BARC075-110.
2, according to the molecular labeling of the described wheat scab resistance main effect QTL linkage of claim 1, it is characterized in that: the disease-resistant strain of described wheat scab peaceful 894037 is to cultivate the somaclone mutant that obtain through young fringe with raising wheat No. 3.
3, molecular labeling according to the described wheat scab resistance main effect QTL linkage of claim 1, it is characterized in that: with wheat cdna group DNA, adopt SSR primer BARC075F:5 '-AGGGTTACAGTTTGCTCTTT TAC-3 ', BARC075R:5-CCCGACGACCTATCTATACTTCTCTA-3 ', Xgwm533F:5 '-AA GGCGAATCAAACGGAATA-3 ', Xgwm533R:5 '-GTTGCTTTAGGGGAAAAGCC-3 ' and BARC133F:5 '-AGCGCTCGAAAAGTCAG-3 ', BARC 133R:5 '-GGCAGG TCCAACTCC AG-3 ' carries out pcr amplification, on amplified production (contains 5.7 gram acrylamides and 0.3 gram methylene diacrylamide) 6% in 100ml polyacrylamide sol solution polyacrylamide gel after the electrophoretic separation, obtain three molecular labelings, size is respectively 110bp, 130bp, 115bp.
4, a kind of application as molecular labeling as described in one of claim 1-3 is characterized in that:
A) will by with peaceful 894037 and derived varieties (being) be male parent or maternal and other wheat hybridizings and multiply to F 2More than generation;
B) separate the genomic DNA of single wheat plant, detect each wheat plant and whether exist with the head blight master and imitate the linked molecular labeling of resistance QTL; Occur simultaneously if any 2 above molecular labelings, predict that then the head blight of this wheat breed reaches anti-level.
5, according to the application of claim 4 the above molecular labeling, it is characterized in that: with molecular labeling Xgwm533-130, BARC133-115, BARC075-110 as assisted Selection be marked at peaceful 894037 and derived varieties (being) use in the wheat scab breeding in anti-source, judge by its genotype whether its derived varieties or strain have scab resistance.
6, according to the application of claim 4 or 5 described molecular labelings, it is characterized in that: used peaceful 894037 derived varieties or strain, be meant that with peaceful 894037 be the parent, by conventional hybridization or adopt tissue culture or adopt anther culture or adopt corn and wheat hybridizing to induce monoploid, wheat breed or the strain that doubles to obtain double haploid or adopt the genetic transforming method acquisition with colchicine again.
CN 03112779 2003-01-28 2003-01-28 Wheat gibberella resistance major effect QTL linked molecular label and its application Pending CN1459226A (en)

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Cited By (11)

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CN100336905C (en) * 2004-05-08 2007-09-12 南京农业大学 Wheat fertility recovery gene molecular mark and its obtaining method
CN100491541C (en) * 2006-09-21 2009-05-27 江苏省农业科学院 Molecular mark closely linked to Sumai No.3 head blight resistance main effect QTL and its application
CN101812517B (en) * 2010-01-18 2012-09-05 华中农业大学 Molecular marker of wheat scab ear rot and seedling rot resistance
CN103224979A (en) * 2013-01-15 2013-07-31 江苏省农业科学院 A pair of primer sequences for screening wheat scab resistance and use thereof
CN104585018A (en) * 2015-01-14 2015-05-06 扬州大学 Cultivating method of wheat-agropyron elongatum FHB (Fusarium head blight)-resistant 7E chromosome long arm translocation line
CN105432463A (en) * 2015-12-21 2016-03-30 天津百利种苗培育有限公司 SSD breeding method for line with disease and insect resistance
CN106995853A (en) * 2017-05-16 2017-08-01 浙江海洋大学 The method for screening the molecular labeling related to few root duckweed leaf growth
CN107760767A (en) * 2017-09-27 2018-03-06 山东省农业科学院生物技术研究中心 A kind of wheat anti gibberellic disease multi-fluorescence SSR marker detection method
CN109355425A (en) * 2018-12-12 2019-02-19 江苏省农业科学院 A kind of and wheat scab resistance QTL chain molecular labeling and its application
CN110093435A (en) * 2015-09-28 2019-08-06 郑州大学 Wheat SSR molecular labeling primer and its screening technique
CN114600767A (en) * 2022-04-07 2022-06-10 四川省农业科学院作物研究所 Method for rapidly breeding wheat durable stripe rust resistant variety

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100336905C (en) * 2004-05-08 2007-09-12 南京农业大学 Wheat fertility recovery gene molecular mark and its obtaining method
CN100491541C (en) * 2006-09-21 2009-05-27 江苏省农业科学院 Molecular mark closely linked to Sumai No.3 head blight resistance main effect QTL and its application
CN101812517B (en) * 2010-01-18 2012-09-05 华中农业大学 Molecular marker of wheat scab ear rot and seedling rot resistance
CN103224979A (en) * 2013-01-15 2013-07-31 江苏省农业科学院 A pair of primer sequences for screening wheat scab resistance and use thereof
CN104585018A (en) * 2015-01-14 2015-05-06 扬州大学 Cultivating method of wheat-agropyron elongatum FHB (Fusarium head blight)-resistant 7E chromosome long arm translocation line
CN110093435A (en) * 2015-09-28 2019-08-06 郑州大学 Wheat SSR molecular labeling primer and its screening technique
CN105432463A (en) * 2015-12-21 2016-03-30 天津百利种苗培育有限公司 SSD breeding method for line with disease and insect resistance
CN106995853A (en) * 2017-05-16 2017-08-01 浙江海洋大学 The method for screening the molecular labeling related to few root duckweed leaf growth
CN106995853B (en) * 2017-05-16 2020-08-25 浙江海洋大学 Method for screening molecular markers related to growth of Lemna minor leaves
CN107760767A (en) * 2017-09-27 2018-03-06 山东省农业科学院生物技术研究中心 A kind of wheat anti gibberellic disease multi-fluorescence SSR marker detection method
CN107760767B (en) * 2017-09-27 2021-04-20 山东省农业科学院生物技术研究中心 Wheat scab-resistant multiple-fluorescence SSR (simple sequence repeat) marker detection method
CN109355425A (en) * 2018-12-12 2019-02-19 江苏省农业科学院 A kind of and wheat scab resistance QTL chain molecular labeling and its application
CN114600767A (en) * 2022-04-07 2022-06-10 四川省农业科学院作物研究所 Method for rapidly breeding wheat durable stripe rust resistant variety

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