CN105175195B - A kind of composite growth-promoting agent promoting tobacco growing - Google Patents
A kind of composite growth-promoting agent promoting tobacco growing Download PDFInfo
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- CN105175195B CN105175195B CN201510672281.XA CN201510672281A CN105175195B CN 105175195 B CN105175195 B CN 105175195B CN 201510672281 A CN201510672281 A CN 201510672281A CN 105175195 B CN105175195 B CN 105175195B
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Abstract
The present invention relates to a kind of composite growth-promoting agent, the microbial inoculum includes bacillus subtilis, bacillus amyloliquefaciens, Bordetella, Sinorhizobium, Pseudomonas alcaligenes, bacterium nitrobacter, actinomyces, sporotrichum thermophile, asparatate sylvite and gibberellin；The growth accelerator of the present invention can improve the economical character of tobacco, such as：Plant height, stem girth, maximum leaf length, maximum width of blade and leaves number；Accumulation of the tobacco in the positions such as root, stem, leaf amount of dry matter can also be improved, composite growth-promoting agent of the invention can be used for improving the growth quality of tobacco.
Description
Technical field：
The present invention relates to a kind of composite growth-promoting agent promoting tobacco growing.
Background technology：
Tobacco is important one of the industrial crops in China, and cultivated area and total output all rank first in the world, tobacco leaf total yield
Amount and 30% or so of the worlds total sales volume Dou Zhan；But with the variation of cultivation condition, the growth of tobacco is gradually affected,
Cause huge economic loss.Soil is the primary condition for determining tobacco production and quality, soil texture, the soil texture, nutrient
Situation, irrigation and drainage, aeration etc. are directly related to the quality and yield of tobacco leaf, and soil pH value, topsoil depth also influence tobacco
Root growth and development.Currently, tobacco plants plantation in China's, which relies primarily on, largely applies chemical fertilizer and pesticide to keep the high yield of tobacco leaf
Amount, serious destruction is caused to tobacco planting soil and environment.
The flue-cured tobacco soil erosion of China's hardboard knot easy to stick is very big, and knotts clay is also more, especially a large amount of applicationizations in recent years
Learn nitrogenous fertilizer makes soil texture wreck although crop yield increases substantially, and hardened soil erosion expands, soil nutrient
Unbalanced supply, great changes will take place for original farmland ecosystem, and active microorganism quantity reduces, the profit of the nutrients such as nitrogen, phosphorus, potassium
It is not high with rate, cause the yield and quality of tobacco to be severely impacted.
Composite growth-promoting agent plays an important role in agricultural system, mainly by being used as bio-feritlizer, generating plant
Object stimulant and as the modes such as the biological control factor to plant growth generate beneficial effect.Composite growth-promoting agent has preferable
Application prospect, the application of chemical fertilizer and pesticide can be largely reduced by being inoculated with composite growth-promoting agent in the soil.Microorganism is in soil
Colonizing in earth contributes to the nutrient for discharging soil fixing, efficiently utilizes soil nitrogen and organic matter；Can also further it improve
Soil physical chemistry structure makes the good crumb structure of Zinc fractions, reduces nutrient loss, improves the deliverability of soil nutrient, can
The absorption rate of fertilizer is significantly improved, to improve ecological environment of soil comprehensively.
Invention content
In order to improve the yield and quality of tobacco, the present invention provides a kind of composite growth-promoting agent, the groups of the growth accelerator
As bacillus subtilis (Bacillus subtilis), bacillus amyloliquefaciens (Bacillus
Amyloliquefaciens), Bordetella (Bordetella sp.), Sinorhizobium (Sinorhizobium sp.),
Pseudomonas alcaligenes (Pseudomonas alcaligenes), bacterium nitrobacter (Nitrobacteriaceae), actinomyces
(Actinomycete), sporotrichum thermophile (Sporotrichum thermophile) and asparatate sylvite and red mould
Element, wherein the content of bacillus subtilis is 10%-25%, and the content of bacillus amyloliquefaciens is 10%-20%, and Boulder is special
The content of Salmonella is 10%-20%, and the content of Sinorhizobium is 10%-20%, and the content of Pseudomonas alcaligenes is 10%-
20%, the content of bacterium nitrobacter is 5%-10%, and the content of actinomyces is 5%-10%, and the content of sporotrichum thermophile is 4%-
8%, separately contain 5~10% asparatate sylvite, gibberellin 0.5~2%.It is preferred that bacillus subtilis 15%, solves starch gemma
Bacillus 15%, Bordetella 15%, Sinorhizobium 15%, Pseudomonas alcaligenes 15%, bacterium nitrobacter 5%, actinomyces
6%, sporotrichum thermophile 5% separately contains 8% asparatate sylvite, gibberellin 1%.
Bacillus subtilis can effectively excite the activity of related resistant enzymes in plant, enhance plant disease and pest resistance
Ability；Bacillus amyloliquefaciens can prevent plant disease, activating soil nutrient；Bordetella helps to enhance in soil
Catalase, invertase, polyphenol oxidase, the activity of urase and intensity of soil respiration, to improve the quality and fertilizer of soil
Power；Sinorhizobium contributes to fixed nitrogen, improves utilization ratio of the plant to nitrogen；Pseudomonas alcaligenes can generate a variety of anti-
Raw element, improves plant nutrient, promotes plant growth, and noxious material in the soil that can degrade, aobvious to Plant growth-promoting effect prophylaxis effect
It writes；Bacterium nitrobacter plays an important role to the cycle of nitrogen；Actinomyces can reduce the pathogenic of phytopathogen and infect
Efficiency inhibits the breeding and growth of pathogen；Sporotrichum thermophile can effectively improve soil, and culture fertility makes crop obtain great number
Yield and fine quality.
The present invention is used in compounding using mentioned microorganism, it is intended to improve the growth quality of tobacco；Conventional method may be used
Spawn incubation is carried out to each microorganism, expands culture, is dried to obtain bacterium powder, and then add each bacterium powder according to aforementioned proportion and be prepared into
For composite bacteria agent, total bacterial content in composite bacteria agent is not less than 1.0 × 1010cfu/g。
Further, growth accelerator of the invention can also simultaneously be used with fertilizer, be used for the growth of tobacco.
Beneficial effects of the present invention：
The composite growth-promoting agent of the present invention can improve the economical character of tobacco, such as：Plant height, stem girth, maximum leaf be long, most
Big leaf width and leaves number；Accumulation of the tobacco in the positions such as root, stem, leaf amount of dry matter can also be improved；The compound rush of the present invention
Growth stimulator can be used for improving the growth quality of tobacco.
Specific implementation mode：
The preparation of 1 composite growth-promoting agent of embodiment：
Each microorganism is cultivated according to this field routine culture mode, using slant medium to bacillus subtilis
Bacterium, bacillus amyloliquefaciens, Bordetella, Sinorhizobium, Pseudomonas alcaligenes, bacterium nitrobacter, actinomyces and thermophilic side
The spore mould culture for carrying out strain after culture, is enlarged culture using fluid nutrient medium, thalline is collected after expanding culture,
It is dry to prepare bacterium powder；Each bacterium powder is compounded to obtain composite bacteria agent, each strain content is as follows in microbial inoculum：Bacillus subtilis 15%,
Bacillus amyloliquefaciens 15%, Bordetella 15%, Sinorhizobium 15%, Pseudomonas alcaligenes 15%, bacterium nitrobacter
5%, actinomyces 6%, sporotrichum thermophile 5% separately contains 8% asparatate sylvite, gibberellin 1%；It compounds in obtained microbial inoculum
Total bacterial content is 5.0 × 1010cfu/g。
2 field experiment of embodiment
2.1 experimental design
This experiment is field experiment, is located at the Henan Province Jiyuan City towns the Wang Wu villages Zhu Quan, and experimental cultivar is Zhongyan-100, three
Gorge pilot experimental cultivar is middle cigarette 102.2 processing (T1 and T2) are set altogether, and experiment is repeated 3 times, random alignment, plot area
0.2 mu.Specific processing is as follows：
T1：Conventional fertilizer application (control)；
T2：The 1 composite growth-promoting agent (10kg microbial inoculums per acre) of conventional fertilizer application+embodiment；
5 plants of listed singlings of representational cigarette strain are chosen, a phase, prosperous long-term and maturity period are rolled into a ball respectively at tobacco grown in field
Measure plant height, stem girth, maximum leaf length and wide, leaves number.
A phase, prosperous long-term and maturity period are rolled into a ball in tobacco grown in field, representational cigarette strain (3 plants) is chosen in each processing,
Whole strain harvest is carried out, separation root, stem, leaf, after being rinsed with clear water, filter paper blots, and root, stem and leaf is separated and is finished at 105 DEG C
15min, is then dried at 60 DEG C, weighs the dry weight of root, stem, leaf respectively.
2.2 interpretation of result
2.2.1 influence of the different disposal to flue-cured tobacco crop field economical character
After tobacco transplant each period tobacco agronomy character index (being shown in Table 1) analysis it is found that group's phase, it is prosperous long-term and at
The ripe phase, plant height, stem girth, maximum leaf be long, the average value of maximum width of blade and leaves number handles T2>T1, significant difference.
Each period tobacco agronomy character after 1 tobacco transplant of table
Note：Each period same row lowercase difference shows that difference reaches 5% level of signifiance in table, similarly hereinafter.
2.2.2 influence of the different disposal to cigarette strain root dry-matter accumulation amount
Each breeding time cigarette strain root dry-matter accumulation amount is shown in Table 2, and moisture, nutrient is absorbed and utilized as plant in tobacco root
Absorb organ and synthesize nicotine synthetic organ, whether its growth and development good, and whether dry-matter accumulation enriches, direct relation
To the power of cigarette strain resistance, the height of tobacco leaf economic benefit and the quality of flavor and taste, T2 processing is increasing Tobacco Root dry matter
Apparent effect is produced in terms of accumulation.
Influence of the 2 composite growth-promoting agent of table to Tobacco Root amount of dry matter
2.2.3 influence of the different disposal to cigarette strain stem substance accumulation
Tobacco stem is connected to root system, supports leaf, flower, fruit, and entire cigarette strain is made to become an organic system, to very well
Adaptation environment, growth and development.Its major physiological function is conveying moisture and nutriment, in the entire growth and development process of cigarette strain
It plays an important role.As shown in Table 3, T2 processing can increase the accumulation of tobacco stem dry matter.
Influence of the 3 composite growth-promoting agent of table to tobacco stem amount of dry matter
2.2.4 influence of the different disposal to cigarette strain dry matter weight of leaf accumulation
Each breeding time cigarette strain dry matter weight of leaf accumulation is shown in Table 4, and for analysis result it is found that in group's phase, T2 processing is more normal
Rule increase leaf dry weight, significantly increase leaf dry weight for a long time prosperous, in the maturity period, the dry-matter accumulation of blade reaches most
Greatly, Huang is fallen through post-mature, you can picking and roasting, therefore, the yield and quality of how many pairs of tobacco leaves of dry-matter accumulation will produce
Raw important influence.
Influence of the 4 composite growth-promoting agent of table to tobacco leaf amount of dry matter
There is certain correlation between tobacco agronomy character and cigarette strain ultimate output, the size of tobacco agronomy character with
The quality of the height of cigarette strain ultimate output and the final economic benefit of tobacco grower has indivisible relationship.It can according to the above analysis
To obtain, composite growth-promoting agent of the invention can effectively improve every economical character index of each growthdevelopmental stage cigarette strain.
The accumulation of each organ dry matter of cigarette strain and nutrient is the important indicator of cigarette strain growth and development, the product of cigarette strain dry matter
Tired amount is related with the environment (climatic factor, edaphic factor etc.) of cigarette strain growth and development, the suction with cigarette strain to nutrient, moisture in soil
It receives and its distribution in cigarette strain body is closely related, while significantly affecting the quality of tobacco leaf.Different times is dry after flue-cured tobacco transplanting
Substance status of accumulating nutrient reflects the growth and development state of plant different parts (root, stem, leaf)；By embodiment 2 it is found that the present invention
Composite growth-promoting agent can improve accumulation of the tobacco in the organs amount of dry matter such as root, stem, leaf.
Claims (4)
1. a kind of composite growth-promoting agent promoting tobacco growing, which is characterized in that the composition and content of the growth accelerator are as follows：
Bacillus subtilis 15%, bacillus amyloliquefaciens 15%, Bordetella 15%, Sinorhizobium 15% produce alkali vacation unit cell
Bacterium 15%, bacterium nitrobacter 5%, actinomyces 6%, sporotrichum thermophile 5%, 8% asparatate sylvite, gibberellin 1%.
2. the fertilizer containing composite growth-promoting agent described in claim 1.
3. application of the composite growth-promoting agent described in claim 1 in terms of promoting tobacco growing.
4. application of the fertilizer in terms of promoting tobacco growing described in claim 2.
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| CN107904179B (en) * | 2017-12-18 | 2020-10-23 | 云南天禾地生物科技股份有限公司 | Cryptococcus laurentii with plant growth promotion and disease resistance and application thereof |
| CN114657087A (en) * | 2021-12-30 | 2022-06-24 | 云南省微生物发酵工程研究中心有限公司 | Microbial fertilizer with function of promoting tobacco plant growth, preparation method and application thereof |
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| CN103787756A (en) * | 2014-01-21 | 2014-05-14 | 中国农业科学院农业资源与农业区划研究所 | Polyamino acid fertilizer auxiliary and preparation method for same |
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1475467A (en) * | 2002-08-14 | 2004-02-18 | 吴晓丽 | Bamboo forest biological fertilizer |
| CN101874507A (en) * | 2010-05-22 | 2010-11-03 | 大连金水微生物工程研究所 | Microbioecological formulation for blueberry cultivation and planting, preparation method and use method thereof |
| CN103052311A (en) * | 2010-07-15 | 2013-04-17 | Fb科技控股公司 | Microorganism compositions and methods |
| CN102417423A (en) * | 2011-07-06 | 2012-04-18 | 山东宝源生物有限公司 | Nutritious compound fertilizer of swamp cabbage |
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