CN105169362A - Biopharmaceutical method for intestinal diseases - Google Patents
Biopharmaceutical method for intestinal diseases Download PDFInfo
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- CN105169362A CN105169362A CN201510559097.4A CN201510559097A CN105169362A CN 105169362 A CN105169362 A CN 105169362A CN 201510559097 A CN201510559097 A CN 201510559097A CN 105169362 A CN105169362 A CN 105169362A
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Abstract
The invention discloses a biopharmaceutical method for intestinal diseases, wherein the method comprises the following steps: crushing plant protein, steaming and digesting the plant protein at high temperature and cooling to 35-45 DEG C; fermenting and domesticating animal-source microorganisms into plant-source microorganisms by virtue of a plant-source culture solution; adding the plant-source microorganisms to plant protein fluid, uniformly stirring, fermenting in a sealed mode at constant temperature of 35-45 DEG C for 72h, hydrolyzing and acidifying, and discharging short-chain fatty acid as a metabolite, filtering the short-chain fatty acid by virtue of a filter screen, and sealing and refrigerating the short-chain fatty acid or freezing the short-chain fatty acid in a container; and adding a calcium chloride solution of 5% to a sodium alginate solution of 1-5%, and uniformly stirring with the obtained short-chain fatty acid and the plant-source microorganisms so as to obtain a microcapsule embedded product. The method disclosed by the invention is simple and easy to operate; and the microcapsule embedded product which is formed by stirring the sodium alginate solution, the calcium chloride solution, the short-chain fatty acid and the plant-source microorganisms, is free from side effect, and is good in effect on preventing and treating various intestinal diseases including enteritis, acute and chronic diarrhea, colitis and the like.
Description
Technical field
The present invention relates to biological pharmacy technical field, be specifically related to a kind of bio-pharmaceuticals method of intestinal tract disease.
Background technology
Whenever seasonal variations, the health of some people can be not suitable with this change in season, the rule of stool will be destroyed, there will be the state of diarrhoea and constipation, most of infectious intestinal disease morbidity has nauseating, vomiting, stomachache, diarrhoea, the bowel symptoms such as inappetence, some is with heating, headache, limbs pain, systemic toxicity profiles symptom, if treatment not in time, serious complication can be caused, especially colitis, dysentery, acute and chronic diarrhea, the intestinal tract diseases such as enteritis, the easy recurrent exerbation delay several months, several years and even many decades, common Therapeutic Method passes through drug administration, physiotherapy, the treatment of the many-side such as diet and control, but often therapeutic effect is unsatisfactory, the easy recurrent exerbation of the state of an illness, treatment does not thoroughly easily cause complication.
Summary of the invention
Technical problem to be solved by this invention is the problem of the Therapeutic Method less effective solving existing intestinal tract disease, the easy recurrent exerbation of the state of an illness.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is to provide a kind of bio-pharmaceuticals method of intestinal tract disease, comprises the following steps:
By corn beans vegetable protein after crushing pulping machine is pulverized and high temperature steaming is sterilized, then be cooled to 35-45 DEG C;
Utilize plant source culture fluid that animal sources fermentable is domesticated for plant source microorganism;
Plant source microorganism is added in vegetable protein liquid, stir, constant temperature 35-45 DEG C sealing and fermenting 72 hours, discharges metabolite short-chain fatty acid by hydrolysis and acidify, after going out short-chain fatty acid by strainer filtering, sealed cold preservation or in a reservoir freezing;
In Solution percentages concentration be 1%-5% sodium alginate soln in add the calcium chloride solution that Solution percentages concentration is 5%, and to stir with the short-chain fatty acid got and plant source microorganism, form microcapsule embedded product;
Wherein, the concrete steps utilizing plant source culture fluid that animal sources fermentable is domesticated for plant source microorganism are as follows:
In the pure water of 100 parts, add the vegetable-derived media of 30-40 part, then add the anaerobe of 3-10 part, stir, constant temperature 35 DEG C of sealing and fermenting 24-48 hours;
Add the vegetable-derived media of 30-40 part and the aerobic microbiological of 3-10 part again, stir, constant temperature 30 DEG C fermentation 12-24 hour, every 15-30 minute oxygenic aeration once; Then, constant temperature 25 DEG C fermentation 8-12 hour, every 30-60 minute oxygenic aeration once; Last natural fermentation 12-24 hour, every 120--240 minute oxygenic aeration once;
Wherein, the ratio of quality and the number of copies of anaerobe and aerobic microbiological is 1:1, the mixed proportion of short-chain fatty acid and plant source microorganism is any, and the ratio of quality and the number of copies of the mixed liquor of sodium alginate soln, calcium chloride solution, short-chain fatty acid and plant source microorganism is 1:1:100.
In technique scheme, described anaerobe and aerobic microbiological comprise: bacillus subtilis, Bacillus licheniformis, Bacillus natto, Brevibacillus laterosporus, bacillus megaterium, bacillusmusilaginosiengineering, photosynthetic bacteria, bacillus polymyxa, Bacillus coagulans, soil bacillus brevis, yeast, EM bacterium, streptococcus faecalis, ocean rhodotorula, bacteriophagic Bdellovibrio, bacillus cereus, lactobacillus, Lactobacillus plantarum, bacillus acidophilus, nitrobacteria, denitrifying bacteria, actinomycetes and fermentable metabolite enzyme, peptide, aminoacid, organic acid and antibiotic.
In technique scheme, described vegetable-derived media is made up of the component of following parts by weight:
Plant-derived protein 84.9-97.6%;
Plant source polysaccharose substance 0.8-14.5%;
Plant extract tea polyphenols 0.1-0.4%;
Sodium chloride powder 0.5-3%;
Described plant source polysaccharose substance comprises the mixture of any one or several in brown sugar, glucose, fructose, multiple monosaccharide and polysaccharide, cellulose, hemicellulose, pectic substance, lignin and starch, and mixed proportion is any.
The present invention, simple to operate easy-to-use, can high efficiency obtain high-quality, high-purity, low cost short-chain fatty acid, plant source microorganism is tamed in the fermentation of animal sources microorganism fungus kind, recover the original wild nature of microorganism, its vigor is strengthened, sodium alginate soln, calcium chloride solution, short-chain fatty acid and plant source microorganism are stirred the microcapsule embedded product formed, be free from side effects, have good preventive and therapeutic action to multiple intestinal tract diseases such as enteritis, dysentery, acute and chronic diarrhea, colitis.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described in detail.
Embodiments provide a kind of bio-pharmaceuticals method of intestinal tract disease, comprise the following steps:
By corn beans vegetable protein after crushing pulping machine is pulverized and high temperature steaming is sterilized, then be cooled to 35-45 DEG C.
Utilize plant source culture fluid that animal sources fermentable is domesticated for plant source microorganism.
Concrete steps are:
In the pure water of 100 parts, add the vegetable-derived media of 30-40 part, then add the anaerobe of 3-10 part, stir, constant temperature 35 DEG C of sealing and fermenting 24-48 hours;
Add the vegetable-derived media of 30-40 part and the aerobic microbiological of 3-10 part again, stir, constant temperature 30 DEG C fermentation 12-24 hour, every 15-30 minute oxygenic aeration once; Then, constant temperature 25 DEG C fermentation 8-12 hour, every 30-60 minute oxygenic aeration once; Last natural fermentation 12-24 hour, every 120--240 minute oxygenic aeration once.
Plant source microorganism is added in vegetable protein liquid, stir, constant temperature 35-45 DEG C sealing and fermenting 72 hours, discharges metabolite short-chain fatty acid by hydrolysis and acidify, after going out short-chain fatty acid by strainer filtering, sealed cold preservation or in a reservoir freezing.
In Solution percentages concentration be 1%-5% sodium alginate soln in add the calcium chloride solution that Solution percentages concentration is 5%, and to stir with the short-chain fatty acid got and plant source microorganism, form microcapsule embedded product.
Above-mentioned microcapsule embedded product is taken by oral.
Wherein, the ratio of quality and the number of copies of anaerobe and aerobic microbiological is 1:1, the mixed proportion of short-chain fatty acid and plant source microorganism is any, and the ratio of quality and the number of copies of the mixed liquor of sodium alginate soln, calcium chloride solution, short-chain fatty acid and plant source microorganism is 1:1:100.
Wherein, anaerobe and aerobic microbiological include but not limited to: bacillus subtilis, Bacillus licheniformis, Bacillus natto, Brevibacillus laterosporus, bacillus megaterium, bacillusmusilaginosiengineering, photosynthetic bacteria, bacillus polymyxa, Bacillus coagulans, soil bacillus brevis, yeast, EM bacterium, streptococcus faecalis, ocean rhodotorula, bacteriophagic Bdellovibrio, bacillus cereus, lactobacillus, Lactobacillus plantarum, bacillus acidophilus, nitrobacteria, denitrifying bacteria, actinomycetes and fermentable metabolite enzyme, peptide, aminoacid, organic acid and antibiotic.
Vegetable-derived media (Powdered) in plant source culture fluid comprises the component of following parts by weight:
Plant-derived protein 84.9-97.6%;
Plant source high-energy polysaccharose substance 0.8-14.5%;
Plant extract tea polyphenols 0.1-0.4%;
Sodium chloride powder 0.5-3%.
Several conventional proportioning is as following table:
Wherein, every 100 grams of plant source culture fluid contain:
Protein: 1.8-18 gram;
Carbohydrate: 1.10-11 gram;
Fat: 0.7-7 gram;
Cellulose: 1.10-11 gram;
Trace element: 0.1-0.5 gram;
All the other are pure water.
Trace element specifically composed as follows, the trace element contained by every 100 grams of vegetable-derived media culture fluid comprises:
Vitamin A: 15-150 microgram;
Vitamin E: 0.8-10 milligram;
Carotene: 90-900 microgram;
Magnesium: 10-45 milligram;
Calcium: 10-50 milligram;
Ferrum: 0.5-2.5 milligram;
Zinc: 0.24-1.2 milligram;
Copper: 0.07-0.35 milligram;
Manganese: 0.09-0.45 milligram;
Potassium: 48-240 milligram;
Phosphorus: 30-150 milligram.
Plant source high-energy polysaccharose substance comprises brown sugar, glucose, fructose, the mixture of any one or several in multiple monosaccharide and polysaccharide fiber element, hemicellulose, pectic substance, lignin and starch, and mixed proportion is any.
Several typical composition composition embodiment of every 100 grams of plant source culture fluid is as following table:
Utilize plant source culture fluid that animal sources fermentable is domesticated for plant source method of microorganism as follows:
The acclimation conditions of A, plant source microorganism:
1) pure water 100 parts, conductivity is necessary for 0;
2) vegetable-derived media 60-80 part, high temperature steaming sterilization in 30 minutes is in advance ready to;
3) animal sources microorganism 6-20 part, anaerobism, aerobic microbiological are separately;
4) tame environment, sealed container, the outside Burdick lamp that only needs to send out ozoniferous, simple, cost is low, without the need to the sterilized space of complexity.
The domestication step of B, plant source microorganism:
1) vegetable-derived media (this enforcement gets 1/3rd) getting 30-40 parts by weight joins in the pure water of 100 parts, then adds the anaerobe of 3-10 part, stirs, constant temperature 35 DEG C of sealing and fermenting 24-48 hours;
2) add the vegetable-derived media (this enforcement gets 1/3rd) of 30-40 and the aerobic microbiological of 3-10 part again, stir, constant temperature 30 DEG C fermentation 12-24 hour, every 15-30 minute oxygenic aeration once;
3) constant temperature 25 DEG C fermentation 8-12 hour, every 30-60 minute oxygenic aeration once;
4) natural fermentation 12-24 hour, every 120--240 minute oxygenic aeration once.
The present invention, pass through sodium alginate soln, calcium chloride solution, the microcapsule embedded product that short-chain fatty acid and plant source microorganism obtain is for reducing the generation of colitis, short-chain fatty acid in human body produces primarily of the carbohydrate per rectum anaerobe zymolysis of not digesting and assimilating, mainly comprise acetic acid, propanoic acid and butanoic acid, short-chain fatty acid not only can be used as the main energy sources of intestinal mucosa cells in colonic lumen, the generation of proinflammatory factor can also be reduced, reduce the generation of colitis, the more important thing is, short-chain fatty acid can play inhibitory action to the propagation of tumor cell, and the differentiation of inducing tumor cell and apoptosis, reduce the probability of canceration.
Meanwhile, pathogenic bacterium can also be suppressed, disease-resistant dye organic acid make enteral PH and EH (oxidation-reduction potential) rise cause sour environment; To pathogenic bacterium as the pathogenic bacterium such as dysentery bacterium, Bacillus typhi, Salmonella paratyphi, Campylobacter, Escherichia coli, bacillus perfringens, bacillus pyocyaneus, staphylococcus have antagonism; Low PH and EH can promote intestinal peristalsis promoting, maintains normal physiological function, prevents pathogenic bacterium definite value, and adjustment intestinal microbial population, improves microecological environment, have good preventive and therapeutic action to multiple intestinal tract diseases such as enteritis, dysentery, acute and chronic diarrhea, colitis; Control the formation of toxicant, lactobacillus can suppress intestinal Putrefying bacteria on the one hand and produce the bacterial reproduction of urease, poisonous ammonia and amine can be become ammonium ion on the other hand, ammonium ion is alkaline, can be combined into nontoxic salt with bacteriogenic acid, reduces the level of ammonia in blood, therefore can be used for the auxiliary treatment that hepatitis, liver cirrhosis, hepatic coma etc. are sick, short-chain fatty acid and lactic acid can also impel the absorption of ammonia and the generation of carbamide, and excrete, the person that is conducive to renal insufficiency; Delay body aging, the short-chain fatty acid that lactobacillus produces and lactic acid can suppress the growth of Putrefying bacteria, thus reduce these bacteriogenic toxic amines, indole, indole, ammonia, scatol, hydrogen sulfide, carcinogen and other toxin, make body aging process become slow; Promote alimentation and regulate endogenous metabolism, short-chain fatty acid can improve some metal ions as the metabolism of calcium, magnesium, ferrum and absorption, after short-chain fatty acid absorbs, its metabolite is utilized in each organ, such as, butyrate can utilize by colon epidermis cell, lactate, propionate and part acetate can be utilized by liver, part acetate also can by muscle and surrounding tissue utilize, so short-chain fatty acid adjustment endogenous metabolism in play an important role.
The present invention is not limited to above-mentioned preferred forms, and anyone should learn the structure change made under enlightenment of the present invention, and every have identical or close technical scheme with the present invention, all falls within protection scope of the present invention.
Claims (3)
1. a bio-pharmaceuticals method for intestinal tract disease, is characterized in that, comprise the following steps:
By corn beans vegetable protein after crushing pulping machine is pulverized and high temperature steaming is sterilized, then be cooled to 35-45 DEG C;
Utilize plant source culture fluid that animal sources fermentable is domesticated for plant source microorganism;
Plant source microorganism is added in vegetable protein liquid, stir, constant temperature 35-45 DEG C sealing and fermenting 72 hours, discharges metabolite short-chain fatty acid by hydrolysis and acidify, after going out short-chain fatty acid by strainer filtering, sealed cold preservation or in a reservoir freezing;
In Solution percentages concentration be 1%-5% sodium alginate soln in add the calcium chloride solution that Solution percentages concentration is 5%, and to stir with the short-chain fatty acid got and plant source microorganism, form microcapsule embedded product;
Wherein, the concrete steps utilizing plant source culture fluid that animal sources fermentable is domesticated for plant source microorganism are as follows:
In the pure water of 100 parts, add the vegetable-derived media of 30-40 part, then add the anaerobe of 3-10 part, stir, constant temperature 35 DEG C of sealing and fermenting 24-48 hours;
Add the vegetable-derived media of 30-40 part and the aerobic microbiological of 3-10 part again, stir, constant temperature 30 DEG C fermentation 12-24 hour, every 15-30 minute oxygenic aeration once; Then, constant temperature 25 DEG C fermentation 8-12 hour, every 30-60 minute oxygenic aeration once; Last natural fermentation 12-24 hour, every 120--240 minute oxygenic aeration once;
Wherein, the ratio of quality and the number of copies of anaerobe and aerobic microbiological is 1:1, the mixed proportion of short-chain fatty acid and plant source microorganism is any, and the ratio of quality and the number of copies of the mixed liquor of sodium alginate soln, calcium chloride solution, short-chain fatty acid and plant source microorganism is 1:1:100.
2. the method for claim 1, it is characterized in that, described anaerobe and aerobic microbiological comprise: bacillus subtilis, Bacillus licheniformis, Bacillus natto, Brevibacillus laterosporus, bacillus megaterium, bacillusmusilaginosiengineering, photosynthetic bacteria, bacillus polymyxa, Bacillus coagulans, soil bacillus brevis, yeast, EM bacterium, streptococcus faecalis, ocean rhodotorula, bacteriophagic Bdellovibrio, bacillus cereus, lactobacillus, Lactobacillus plantarum, bacillus acidophilus, nitrobacteria, denitrifying bacteria, actinomycetes and fermentable metabolite enzyme, peptide, aminoacid, organic acid and antibiotic.
3. the method for claim 1, is characterized in that, described vegetable-derived media is made up of the component of following parts by weight:
Plant-derived protein 84.9-97.6%;
Plant source polysaccharose substance 0.8-14.5%;
Plant extract tea polyphenols 0.1-0.4%;
Sodium chloride powder 0.5-3%;
Described plant source polysaccharose substance comprises the mixture of any one or several in brown sugar, glucose, fructose, multiple monosaccharide and polysaccharide, cellulose, hemicellulose, pectic substance, lignin and starch, and mixed proportion is any.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106811437A (en) * | 2017-04-05 | 2017-06-09 | 新疆金兰德泰环保科技有限公司 | A kind of composite bacteria agent and application |
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| CN103704718A (en) * | 2013-12-23 | 2014-04-09 | 安徽大学 | Preparation method of bacillus subtilis microcapsule with high viable count within shelf life |
| CN103815018A (en) * | 2014-03-06 | 2014-05-28 | 四川红草地农业开发有限公司 | Yak yoghourt and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101402510A (en) * | 2008-11-14 | 2009-04-08 | 盐城海德能水处理环保工程有限公司 | Monotubular anaerobic-aerobic composite microencapsulation biological fluidized bed automatic control apparatus |
| CN103704719A (en) * | 2013-12-23 | 2014-04-09 | 安徽大学 | Preparation method for probiotics microcapsule with high viable count |
| CN103704718A (en) * | 2013-12-23 | 2014-04-09 | 安徽大学 | Preparation method of bacillus subtilis microcapsule with high viable count within shelf life |
| CN103815018A (en) * | 2014-03-06 | 2014-05-28 | 四川红草地农业开发有限公司 | Yak yoghourt and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106811437A (en) * | 2017-04-05 | 2017-06-09 | 新疆金兰德泰环保科技有限公司 | A kind of composite bacteria agent and application |
| CN106811437B (en) * | 2017-04-05 | 2020-05-12 | 新疆金兰德一泰环保科技有限公司 | Complex microbial inoculant and application thereof |
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Application publication date: 20151223 |