CN105166626A - Preparation method of rice bran polysaccharide buccal tablets - Google Patents
Preparation method of rice bran polysaccharide buccal tablets Download PDFInfo
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- CN105166626A CN105166626A CN201510630562.9A CN201510630562A CN105166626A CN 105166626 A CN105166626 A CN 105166626A CN 201510630562 A CN201510630562 A CN 201510630562A CN 105166626 A CN105166626 A CN 105166626A
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 83
- 235000009566 rice Nutrition 0.000 title claims abstract description 83
- 150000004676 glycans Chemical class 0.000 title claims abstract description 43
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 42
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 42
- 239000006189 buccal tablet Substances 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 82
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000000502 dialysis Methods 0.000 claims abstract description 14
- 238000003756 stirring Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000005238 degreasing Methods 0.000 claims abstract description 9
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 8
- 230000010355 oscillation Effects 0.000 claims abstract description 8
- 239000013049 sediment Substances 0.000 claims abstract description 8
- 239000000243 solution Substances 0.000 claims description 45
- 229940046011 buccal tablet Drugs 0.000 claims description 23
- 210000000170 cell membrane Anatomy 0.000 claims description 19
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 18
- 238000010792 warming Methods 0.000 claims description 18
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 17
- 230000008569 process Effects 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 13
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 12
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid group Chemical class C(CC(O)(C(=O)O)CC(=O)O)(=O)O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- 239000012141 concentrate Substances 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 12
- 238000007670 refining Methods 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- 229960001760 dimethyl sulfoxide Drugs 0.000 claims description 7
- 235000019441 ethanol Nutrition 0.000 claims description 7
- 238000000703 high-speed centrifugation Methods 0.000 claims description 7
- 239000011122 softwood Substances 0.000 claims description 7
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 6
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 6
- AEMOLEFTQBMNLQ-BKBMJHBISA-N alpha-D-galacturonic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-BKBMJHBISA-N 0.000 claims description 6
- 239000007853 buffer solution Substances 0.000 claims description 6
- 235000015165 citric acid Nutrition 0.000 claims description 6
- 235000009508 confectionery Nutrition 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims description 6
- 239000008101 lactose Substances 0.000 claims description 6
- 238000002386 leaching Methods 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 6
- 229920001277 pectin Polymers 0.000 claims description 6
- 235000010987 pectin Nutrition 0.000 claims description 6
- 239000001814 pectin Substances 0.000 claims description 6
- 238000010992 reflux Methods 0.000 claims description 6
- 229910000033 sodium borohydride Inorganic materials 0.000 claims description 6
- 239000012279 sodium borohydride Substances 0.000 claims description 6
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 claims description 6
- 235000019345 sodium thiosulphate Nutrition 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 6
- 210000000214 mouth Anatomy 0.000 abstract description 7
- 238000010579 first pass effect Methods 0.000 abstract description 6
- 210000004185 liver Anatomy 0.000 abstract description 6
- KFSLWBXXFJQRDL-UHFFFAOYSA-N Peracetic acid Chemical compound CC(=O)OO KFSLWBXXFJQRDL-UHFFFAOYSA-N 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 210000002421 cell wall Anatomy 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 abstract 1
- 239000007779 soft material Substances 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 230000036039 immunity Effects 0.000 description 6
- 230000003203 everyday effect Effects 0.000 description 4
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 101150076104 EAT2 gene Proteins 0.000 description 1
- 108010050181 aleurone Proteins 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a preparation method of rice bran polysaccharide buccal tablets, and belongs to the technical field of agriculture product processing. The method comprises the steps that rice bran is used as a raw material, alcohol washing and degreasing are performed, a dimethyl sulfoxide solution is used for ultrasonic oscillation, then, dialysis is performed through flowing distilled water to obtain rice bran cell walls, then peracetic acid and an EDTA solution are used for degreasing, then, concentration processing and high-speed centrifugal are performed to obtain sediments, auxiliary materials are added for stirring to obtain a soft material, and finally tableting is performed to obtain the rice bran polysaccharide buccal tablets. According to the embodiment, the method is unique and novel, and the obtained rice bran polysaccharide buccal tablets are fine in taste and convenient for a user to carry and take, can directly act on the oral cavity and the pharyngeal with no first-pass effect of the liver, and is rapid in absorption and fast in onset.
Description
Technical field
The invention discloses a kind of preparation method of rice bran polysaccharide buccal tablet, belong to technical field of agricultural product process.
Background technology
Paddy is China the 1st generalized grain kind.Current annual output about 2.06 hundred million tons.Account for 42% of national total output of grain, rice yield accounts for 37% of total output of grain in the world.Paddy will remove shell and account for the pericarp of gross weight about 10%, seed coat, perisperm, mush layer and embryo in the process being processed into polished rice, traditional rice bran i.e. existing national standards rice bran are mainly processed into by pericarp, seed coat, perisperm, aleurone and embryo, therefore a small amount of rice husk and a certain amount of dust and microorganism can be infiltrated in process, so can only feed be used for, it is the principal by product of paddy processing.
According to data, containing fat, protein, water-soluble polysaccharide, dietary fiber, mineral matter and multivitamin in rice bran, the nutrition of paddy 64% is included in rice bran, rice bran contains abundant trophic factors, wherein protein content about 15 ~ 20%, fat content about 16 ~ 24%, heat is about 125.1KG/g, therefore Appropriate application rice bran, avoids the wasting of resources to be very important thing.
Contain polysaccharide and albumen in rice bran in addition, rice bran polysaccharide is present in paddy caryopsis cortex, is not yet to be developed and one of main nutrient composition of extensive use at present.Water-soluble rice bran polysaccharide is different with general equal glycan, there is physiologically actives such as improving immunocompetence, and have significant biologically active and health care, not only there is the physiological function that polysaccharide has, but also there is the several functions such as antitumor, hypoglycemic, norcholesterol and enhancing immunity, if but rice bran polysaccharide directly eats after extraction, be unfavorable for oral cavity and pharyngeal, absorption is slow, onset is also slower, cause poor effect, the wasting of resources, and cannot be easy to carry, therefore, developing a kind of rice bran polysaccharide product is a significantly thing.
Summary of the invention
The technical problem that the present invention mainly solves: to human body, there is nutritional labeling widely for current rice bran polysaccharide, if but rice bran polysaccharide directly eats after extraction, be unfavorable for absorption of human body, not only onset, absorb slow, and cannot be easy to carry, cause the wasting of resources, the shortcoming of poor effect, provide a kind of preparation method of rice bran polysaccharide buccal tablet, the method take rice bran as raw material, by alcohol wash degreasing, rice bran cell membrane is obtained again with the distill water dialysis that flows after dimethyl sulphoxide solution sonic oscillation, peracetic acid and EDTA solution come unstuck afterwards, concentration high speed centrifugation obtains sediment afterwards, add auxiliary material and stir obtained softwood, namely last compressing tablet obtains a kind of rice bran polysaccharide buccal tablet.The rice bran polysaccharide buccal tablet that the method is made, not only delicate mouthfeel, it is all convenient to carry and take, and without the first pass effect of liver, and directly act on oral cavity and pharyngeal, absorb fast, onset is rapid.
In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is:
(1) 100 ~ 200g rice bran is taken, put into airslide disintegrating mill to pulverize, and cross 60 mesh standard sieves, move into afterwards in the round-bottomed flask with reflux condensing tube, add the ethanolic solution that 500 ~ 600mL mass concentration is 30%, move into water-bath, after being warming up to 80 ~ 90 DEG C of backflow degreasing 2 ~ 3h, filtration obtains filter residue, is moved into thermostatic drying chamber, dry 1 ~ 2h at 50 ~ 60 DEG C;
(2) the above-mentioned pretreated rice bran of 100 ~ 120g is got, the mass concentration containing 5 ~ 6mmol/L sodium thiosulfate adding its 20 times of volumes is the sodium dodecyl sulfate solution of 2%, move into water-bath, be warming up to 50 ~ 60 DEG C, insulation leaves standstill 3 ~ 4h, put into horizontal high-speed centrifuge afterwards, with the rotating speed high speed centrifugation process 30 ~ 40min of 8000 ~ 9000r/min, be separated removal supernatant and be precipitated thing;
(3) mass concentration sediment obtained above being joined its 20 times of volumes is in the dimethyl sulphoxide solution of 90%, put into ultrasonator, sonic oscillation 10 ~ 20min, ultrasonic power is 100 ~ 200W, and supersonic frequency is 25 ~ 35kPa, afterwards suspension is placed on magnetic stirrer, stir 12 ~ 18h with the rotating speed of 300 ~ 400r/min, pour bag filter into afterwards, move into baking oven with after flowing distill water dialysis 5 ~ 6h, at 60 ~ 70 DEG C, dry 3 ~ 4h, obtains rice bran cell membrane;
(4) take the above-mentioned obtained dry rice bran cell membrane of 20 ~ 30g and put into 500mL beaker, to add 200mL concentration be 50mmol/L edta solution and concentration is the buffer solution that 0.05mol/L acetum is made, oil bath is warming up to 90 ~ 100 DEG C, lixiviate 2 ~ 4h, to remove pectic substance;
(5) be 20mmol/L by the rice bran cell membrane continuation 200mL concentration after above-mentioned removal pectin, sodium borohydride solution room temperature under lixiviate 4 ~ 6h, filter and remove filter residue, leaching liquor mass concentration is acetic acid solution adjustment pH to 6 ~ 7 of 30%, load bag filter afterwards, with after flowing distill water dialysis 5 ~ 10h, dialysate is moved into concentration tank, reduce pressure to 900 ~ 1000Pa in tank, carry out reduced pressure concentration process, obtain pureed concentrate;
(6) in concentrate obtained above, add the absolute ethyl alcohol of its volume 4 ~ 5 times, move in ice bath pot after stirring with glass bar, leave standstill 4 ~ 5h at 4 ~ 6 DEG C after, move into supercentrifuge, with the rotating speed centrifugal treating 10 ~ 20min of 5000 ~ 6000r/min, be separated removal supernatant and be precipitated, to precipitate and rinse 2 ~ 3 times with absolute ethyl alcohol, acetone and absolute ether successively, put into thermostatic drying chamber afterwards, at 60 ~ 70 DEG C, dry 5 ~ 6h, obtains refining rice bran polysaccharide;
(7) 50 ~ 54% refining rice bran polysaccharide, 10 ~ 12% lactose, 12 ~ 13% sweet mellow wine, 15 ~ 17% citric acids and 12 ~ 13% dolomols are taken by mass percentage, join mix and blend in 50 ~ 100mL ethanol solution obtain buccal tablet softwood and put into tabletting machine, packaging, obtains a kind of rice bran polysaccharide buccal tablet.
Application process of the present invention is: time edible, being positioned in mouth by a kind of rice bran polysaccharide buccal tablet obtained by the present invention slowly makes it melt, and wherein eat 1 ~ 3 time every day, each amount is 1 ~ 2, edible 5 ~ 10 days continuously, immunity is improved significantly.
The invention has the beneficial effects as follows:
(1) a kind of rice bran polysaccharide buccal tablet of obtaining of the present invention, not only delicate mouthfeel, it is all convenient to carry and take, and without the first pass effect of liver, and directly act on cavity and pharyngeal, absorb fast, onset is rapid;
(2) effectively can improve body immunity after eating, have significant health care.
Detailed description of the invention
First 100 ~ 200g rice bran is taken, put into airslide disintegrating mill to pulverize, and cross 60 mesh standard sieves, move into afterwards in the round-bottomed flask with reflux condensing tube, add the ethanolic solution that 500 ~ 600mL mass concentration is 30%, move into water-bath, after being warming up to 80 ~ 90 DEG C of backflow degreasing 2 ~ 3h, filtration obtains filter residue, is moved into thermostatic drying chamber, dry 1 ~ 2h at 50 ~ 60 DEG C; Then the above-mentioned pretreated rice bran of 100 ~ 120g is got, the mass concentration containing 5 ~ 6mmol/L sodium thiosulfate adding its 20 times of volumes is the sodium dodecyl sulfate solution of 2%, move into water-bath, be warming up to 50 ~ 60 DEG C, insulation leaves standstill 3 ~ 4h, put into horizontal high-speed centrifuge afterwards, with the rotating speed high speed centrifugation process 30 ~ 40min of 8000 ~ 9000r/min, be separated removal supernatant and be precipitated thing; The mass concentration afterwards sediment obtained above being joined its 20 times of volumes is in the dimethyl sulphoxide solution of 90%, put into ultrasonator, sonic oscillation 10 ~ 20min, ultrasonic power is 100 ~ 200W, and supersonic frequency is 25 ~ 35kPa, afterwards suspension is placed on magnetic stirrer, stir 12 ~ 18h with the rotating speed of 300 ~ 400r/min, pour bag filter into afterwards, move into baking oven with after flowing distill water dialysis 5 ~ 6h, at 60 ~ 70 DEG C, dry 3 ~ 4h, obtains rice bran cell membrane; Take the above-mentioned obtained dry rice bran cell membrane of 20 ~ 30g subsequently and put into 500mL beaker, to add 200mL concentration be 50mmol/L edta solution and concentration is the buffer solution that 0.05mol/L acetum is made, oil bath is warming up to 90 ~ 100 DEG C, lixiviate 2 ~ 4h, to remove pectic substance; Following is 20mmol/L by the rice bran cell membrane continuation 200mL concentration after above-mentioned removal pectin, sodium borohydride solution room temperature under lixiviate 4 ~ 6h, filter and remove filter residue, leaching liquor mass concentration is acetic acid solution adjustment pH to 6 ~ 7 of 30%, load bag filter afterwards, with after flowing distill water dialysis 5 ~ 10h, dialysate is moved into concentration tank, reduce pressure to 900 ~ 1000Pa in tank, carry out reduced pressure concentration process, obtain pureed concentrate; The absolute ethyl alcohol of its volume 4 ~ 5 times is added in concentrate obtained above, move in ice bath pot after stirring with glass bar, leave standstill 4 ~ 5h at 4 ~ 6 DEG C after, move into supercentrifuge, with the rotating speed centrifugal treating 10 ~ 20min of 5000 ~ 6000r/min, be separated removal supernatant and be precipitated, to precipitate and rinse 2 ~ 3 times with absolute ethyl alcohol, acetone and absolute ether successively, put into thermostatic drying chamber afterwards, at 60 ~ 70 DEG C, dry 5 ~ 6h, obtains refining rice bran polysaccharide; Finally take 50 ~ 54% refining rice bran polysaccharide, 10 ~ 12% lactose, 12 ~ 13% sweet mellow wine, 15 ~ 17% citric acids and 12 ~ 13% dolomols by mass percentage, join mix and blend in 50 ~ 100mL ethanol solution obtain buccal tablet softwood and put into tabletting machine, packaging, obtains a kind of rice bran polysaccharide buccal tablet.
Example 1
First 100g rice bran is taken, put into airslide disintegrating mill to pulverize, and cross 60 mesh standard sieves, move into afterwards in the round-bottomed flask with reflux condensing tube, add the ethanolic solution that 500mL mass concentration is 30%, move into water-bath, after being warming up to 80 DEG C of backflow degreasing 2h, filtration obtains filter residue, is moved into thermostatic drying chamber, dry 1h at 50 DEG C; Then the above-mentioned pretreated rice bran of 100g is got, the mass concentration containing 5mmol/L sodium thiosulfate adding its 20 times of volumes is the sodium dodecyl sulfate solution of 2%, move into water-bath, be warming up to 50 DEG C, insulation leaves standstill 3h, put into horizontal high-speed centrifuge afterwards, with the rotating speed high speed centrifugation process 30min of 8000r/min, be separated removal supernatant and be precipitated thing; The mass concentration afterwards sediment obtained above being joined its 20 times of volumes is in the dimethyl sulphoxide solution of 90%, put into ultrasonator, sonic oscillation 10min, ultrasonic power is 100W, and supersonic frequency is 25kPa, afterwards suspension is placed on magnetic stirrer, stir 12h with the rotating speed of 300r/min, pour bag filter into afterwards, move into baking oven with after flowing distill water dialysis 5h, dry 3h at 60 DEG C, obtains rice bran cell membrane; Take the above-mentioned obtained dry rice bran cell membrane of 20g subsequently and put into 500mL beaker, to add 200mL concentration be 50mmol/L edta solution and concentration is the buffer solution that 0.05mol/L acetum is made, oil bath is warming up to 90 DEG C, lixiviate 2h, to remove pectic substance; Following is 20mmol/L by the rice bran cell membrane continuation 200mL concentration after above-mentioned removal pectin, sodium borohydride solution room temperature under lixiviate 4h, filter and remove filter residue, leaching liquor mass concentration is the acetic acid solution adjustment pH to 6 of 30%, load bag filter afterwards, with after flowing distill water dialysis 5h, dialysate is moved into concentration tank, in reduction tank, pressure is to 900Pa, carry out reduced pressure concentration process, obtain pureed concentrate; The absolute ethyl alcohol of its volume 4 times is added in concentrate obtained above, move in ice bath pot after stirring with glass bar, leave standstill 4h at 4 DEG C after, move into supercentrifuge, with the rotating speed centrifugal treating 10min of 5000r/min, be separated removal supernatant and be precipitated, precipitation is rinsed 2 times with absolute ethyl alcohol, acetone and absolute ether successively, put into thermostatic drying chamber afterwards, dry 5h at 60 DEG C, obtains refining rice bran polysaccharide; Finally take 50% refining rice bran polysaccharide, 10% lactose, 12% sweet mellow wine, 15% citric acid and 12% dolomol by mass percentage, join mix and blend in 50mL ethanol solution obtain buccal tablet softwood and put into tabletting machine, packaging, obtains a kind of rice bran polysaccharide buccal tablet.
This example operation is simply novel, time edible, be positioned in mouth by a kind of rice bran polysaccharide buccal tablet obtained by the present invention and slowly make it melt, wherein eat 1 time every day, each amount is 1, edible 5 days continuously, not only delicate mouthfeel, it is all convenient to carry and take, and without the first pass effect of liver, directly act on cavity and pharyngeal, absorb fast, onset is rapid, and immunity is improved significantly.
Example 2
First 150g rice bran is taken, put into airslide disintegrating mill to pulverize, and cross 60 mesh standard sieves, move into afterwards in the round-bottomed flask with reflux condensing tube, add the ethanolic solution that 550mL mass concentration is 30%, move into water-bath, after being warming up to 85 DEG C of backflow degreasing 2.5h, filtration obtains filter residue, is moved into thermostatic drying chamber, dry 1.5h at 55 DEG C; Then the above-mentioned pretreated rice bran of 110g is got, the mass concentration containing 5.5mmol/L sodium thiosulfate adding its 20 times of volumes is the sodium dodecyl sulfate solution of 2%, move into water-bath, be warming up to 55 DEG C, insulation leaves standstill 3.5h, put into horizontal high-speed centrifuge afterwards, with the rotating speed high speed centrifugation process 35min of 8500r/min, be separated removal supernatant and be precipitated thing; The mass concentration afterwards sediment obtained above being joined its 20 times of volumes is in the dimethyl sulphoxide solution of 90%, put into ultrasonator, sonic oscillation 15min, ultrasonic power is 150W, and supersonic frequency is 30kPa, afterwards suspension is placed on magnetic stirrer, stir 15h with the rotating speed of 350r/min, pour bag filter into afterwards, move into baking oven with after flowing distill water dialysis 5.5h, dry 3.5h at 65 DEG C, obtains rice bran cell membrane; Take the above-mentioned obtained dry rice bran cell membrane of 25g subsequently and put into 500mL beaker, to add 200mL concentration be 50mmol/L edta solution and concentration is the buffer solution that 0.05mol/L acetum is made, oil bath is warming up to 95 DEG C, lixiviate 3h, to remove pectic substance; Following is 20mmol/L by the rice bran cell membrane continuation 200mL concentration after above-mentioned removal pectin, sodium borohydride solution room temperature under lixiviate 5h, filter and remove filter residue, leaching liquor mass concentration is the acetic acid solution adjustment pH to 6.5 of 30%, load bag filter afterwards, with after flowing distill water dialysis 8h, dialysate is moved into concentration tank, in reduction tank, pressure is to 950Pa, carry out reduced pressure concentration process, obtain pureed concentrate; The absolute ethyl alcohol of its volume 4.5 times is added in concentrate obtained above, move in ice bath pot after stirring with glass bar, leave standstill 4.5h at 5 DEG C after, move into supercentrifuge, with the rotating speed centrifugal treating 15min of 5500r/min, be separated removal supernatant and be precipitated, precipitation is rinsed 2 times with absolute ethyl alcohol, acetone and absolute ether successively, put into thermostatic drying chamber afterwards, dry 5.5h at 65 DEG C, obtains refining rice bran polysaccharide; Finally take 52% refining rice bran polysaccharide, 11% lactose, 12.5% sweet mellow wine, 16% citric acid and 12.5% dolomol by mass percentage, join mix and blend in 75mL ethanol solution obtain buccal tablet softwood and put into tabletting machine, packaging, obtains a kind of rice bran polysaccharide buccal tablet.
This example operation is simply novel, time edible, be positioned in mouth by a kind of rice bran polysaccharide buccal tablet obtained by the present invention and slowly make it melt, wherein eat 2 times every day, each amount is 1.5, edible 8 days continuously, not only delicate mouthfeel, it is all convenient to carry and take, and without the first pass effect of liver, directly act on cavity and pharyngeal, absorb fast, onset is rapid, and immunity is improved significantly.
Example 3
First 200g rice bran is taken, put into airslide disintegrating mill to pulverize, and cross 60 mesh standard sieves, move into afterwards in the round-bottomed flask with reflux condensing tube, add the ethanolic solution that 600mL mass concentration is 30%, move into water-bath, after being warming up to 90 DEG C of backflow degreasing 3h, filtration obtains filter residue, is moved into thermostatic drying chamber, dry 2h at 60 DEG C; Then the above-mentioned pretreated rice bran of 120g is got, the mass concentration containing 6mmol/L sodium thiosulfate adding its 20 times of volumes is the sodium dodecyl sulfate solution of 2%, move into water-bath, be warming up to 60 DEG C, insulation leaves standstill 4h, put into horizontal high-speed centrifuge afterwards, with the rotating speed high speed centrifugation process 40min of 9000r/min, be separated removal supernatant and be precipitated thing; The mass concentration afterwards sediment obtained above being joined its 20 times of volumes is in the dimethyl sulphoxide solution of 90%, put into ultrasonator, sonic oscillation 20min, ultrasonic power is 200W, and supersonic frequency is 35kPa, afterwards suspension is placed on magnetic stirrer, stir 18h with the rotating speed of 400r/min, pour bag filter into afterwards, move into baking oven with after flowing distill water dialysis 6h, dry 4h at 70 DEG C, obtains rice bran cell membrane; Take the above-mentioned obtained dry rice bran cell membrane of 30g subsequently and put into 500mL beaker, to add 200mL concentration be 50mmol/L edta solution and concentration is the buffer solution that 0.05mol/L acetum is made, oil bath is warming up to 100 DEG C, lixiviate 4h, to remove pectic substance; Following is 20mmol/L by the rice bran cell membrane continuation 200mL concentration after above-mentioned removal pectin, sodium borohydride solution room temperature under lixiviate 6h, filter and remove filter residue, leaching liquor mass concentration is the acetic acid solution adjustment pH to 7 of 30%, load bag filter afterwards, with after flowing distill water dialysis 10h, dialysate is moved into concentration tank, in reduction tank, pressure is to 1000Pa, carry out reduced pressure concentration process, obtain pureed concentrate; The absolute ethyl alcohol of its volume 5 times is added in concentrate obtained above, move in ice bath pot after stirring with glass bar, leave standstill 5h at 6 DEG C after, move into supercentrifuge, with the rotating speed centrifugal treating 20min of 6000r/min, be separated removal supernatant and be precipitated, precipitation is rinsed 3 times with absolute ethyl alcohol, acetone and absolute ether successively, put into thermostatic drying chamber afterwards, dry 6h at 70 DEG C, obtains refining rice bran polysaccharide; Finally take 54% refining rice bran polysaccharide, 12% lactose, 13% sweet mellow wine, 17% citric acid and 13% dolomol by mass percentage, join mix and blend in 100mL ethanol solution obtain buccal tablet softwood and put into tabletting machine, packaging, obtains a kind of rice bran polysaccharide buccal tablet.
This example operation is simply novel, time edible, be positioned in mouth by a kind of rice bran polysaccharide buccal tablet obtained by the present invention and slowly make it melt, wherein eat 3 times every day, each amount is 2, edible 10 days continuously, not only delicate mouthfeel, it is all convenient to carry and take, and without the first pass effect of liver, directly act on cavity and pharyngeal, absorb fast, onset is rapid, and immunity is improved significantly.
Claims (1)
1. a preparation method for rice bran polysaccharide buccal tablet, is characterized in that concrete preparation process is:
(1) 100 ~ 200g rice bran is taken, put into airslide disintegrating mill to pulverize, and cross 60 mesh standard sieves, move into afterwards in the round-bottomed flask with reflux condensing tube, add the ethanolic solution that 500 ~ 600mL mass concentration is 30%, move into water-bath, after being warming up to 80 ~ 90 DEG C of backflow degreasing 2 ~ 3h, filtration obtains filter residue, is moved into thermostatic drying chamber, dry 1 ~ 2h at 50 ~ 60 DEG C;
(2) the above-mentioned pretreated rice bran of 100 ~ 120g is got, the mass concentration containing 5 ~ 6mmol/L sodium thiosulfate adding its 20 times of volumes is the sodium dodecyl sulfate solution of 2%, move into water-bath, be warming up to 50 ~ 60 DEG C, insulation leaves standstill 3 ~ 4h, put into horizontal high-speed centrifuge afterwards, with the rotating speed high speed centrifugation process 30 ~ 40min of 8000 ~ 9000r/min, be separated removal supernatant and be precipitated thing;
(3) mass concentration sediment obtained above being joined its 20 times of volumes is in the dimethyl sulphoxide solution of 90%, put into ultrasonator, sonic oscillation 10 ~ 20min, ultrasonic power is 100 ~ 200W, and supersonic frequency is 25 ~ 35kPa, afterwards suspension is placed on magnetic stirrer, stir 12 ~ 18h with the rotating speed of 300 ~ 400r/min, pour bag filter into afterwards, move into baking oven with after flowing distill water dialysis 5 ~ 6h, at 60 ~ 70 DEG C, dry 3 ~ 4h, obtains rice bran cell membrane;
(4) take the above-mentioned obtained dry rice bran cell membrane of 20 ~ 30g and put into 500mL beaker, to add 200mL concentration be 50mmol/L edta solution and concentration is the buffer solution that 0.05mol/L acetum is made, oil bath is warming up to 90 ~ 100 DEG C, lixiviate 2 ~ 4h, to remove pectic substance;
(5) be 20mmol/L by the rice bran cell membrane continuation 200mL concentration after above-mentioned removal pectin, sodium borohydride solution room temperature under lixiviate 4 ~ 6h, filter and remove filter residue, leaching liquor mass concentration is acetic acid solution adjustment pH to 6 ~ 7 of 30%, load bag filter afterwards, with after flowing distill water dialysis 5 ~ 10h, dialysate is moved into concentration tank, reduce pressure to 900 ~ 1000Pa in tank, carry out reduced pressure concentration process, obtain pureed concentrate;
(6) in concentrate obtained above, add the absolute ethyl alcohol of its volume 4 ~ 5 times, move in ice bath pot after stirring with glass bar, leave standstill 4 ~ 5h at 4 ~ 6 DEG C after, move into supercentrifuge, with the rotating speed centrifugal treating 10 ~ 20min of 5000 ~ 6000r/min, be separated removal supernatant and be precipitated, to precipitate and rinse 2 ~ 3 times with absolute ethyl alcohol, acetone and absolute ether successively, put into thermostatic drying chamber afterwards, at 60 ~ 70 DEG C, dry 5 ~ 6h, obtains refining rice bran polysaccharide;
(7) 50 ~ 54% refining rice bran polysaccharide, 10 ~ 12% lactose, 12 ~ 13% sweet mellow wine, 15 ~ 17% citric acids and 12 ~ 13% dolomols are taken by mass percentage, join mix and blend in 50 ~ 100mL ethanol solution obtain buccal tablet softwood and put into tabletting machine, packaging, obtains a kind of rice bran polysaccharide buccal tablet.
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