CN105165830A - Bactericide compounded by eupatorium adenophorum extract and mefenoxam - Google Patents
Bactericide compounded by eupatorium adenophorum extract and mefenoxam Download PDFInfo
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- CN105165830A CN105165830A CN201510536151.3A CN201510536151A CN105165830A CN 105165830 A CN105165830 A CN 105165830A CN 201510536151 A CN201510536151 A CN 201510536151A CN 105165830 A CN105165830 A CN 105165830A
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- metalaxyl
- ketone
- bactericide
- herba lycopi
- carbonyl
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Abstract
The present invention discloses a bactericide compounded by eupatorium adenophorum extract and mefenoxam. Active ingredients of the bactericide are mefenoxam and lycopus lucidus ketone. The bactericide disclosed by the present invention is compounded by natural plant extract eupatorium adenophorum and mefenoxama, so that the usage amount of a conventional pesticide mefenoxama is reduced, and meanwhile the using effect is increased. The compound bactericide is a bactericide with high efficiency, low toxicity and quick degradation speed, so that plant fungal diseases including pepper phytophthora, pythiummyriotylum and the like can be effectively prevented and cured.
Description
Technical field
The present invention relates to a kind of composite bactericide, particularly a kind of purple stem boneset extract and the composite bactericide of Metalaxyl-M.
Background technology
Eupatorium adenophorum is a kind of perennial herb or becomes suffruticose plant.The sturdy prosperity of rhizome, uprightly, plant height 30-200 centimetre, branch to life, tiltedly on, stem purple, by white or rust pubescence.A kind of common weeds in south China growth.The extract that the distinct methods of Eupatorium adenophorum extracts has different functions, patent 200810058143.2 reports purple stem boneset extract can as root-growing agent, patent 201310007596.3 reports that the extract of Eupatorium adenophorum can kill roundworm egg, patent 02113396.4 reports that Eupatorium adenophorum powder has antiheumatic effect, patent 200810008066.X reports the application of Eupatorium adenophorum extract as anti-fouling paint antifoulant, the material extracted in these patents does not all have its definite material clear and definite to form, patent 201310184592.2 discloses a kind of method extracting Herba Lycopi's copper compound in Eupatorium adenophorum, specify that the compound extracted is Z6:2-deoxidation-2-acetoxyl group-9-carbonyl Herba Lycopi ketone, Z7:9-carbonyl-10H β Herba Lycopi ketone, Z8:9-carbonyl-10H α Herba Lycopi ketone, Z9:9-carbonyl-10, 11 dehydrogenation Herba Lycopi ketone.But, to these compounds extracted, there is being not very clear of which concrete functional study.
Metalaxyl-M; chemical name N-(2-Methoxyacetyl)-N-(2; 6-xylyl)-D-alanine methyl esters; it is a kind of effective preparation preventing and treating the plant mycosises such as flowers downy mildew, Chinese cabbage downy mildew, cucumber downy mildew, tomato late blight morning; but the usage amount of Metalaxyl-M is larger; be not easy degraded, the effect of control is not thorough, and these all limit it in extensive use agriculturally.
The composite usage amount that can reduce traditional agricultural chemicals of natural plants bactericide and traditional agricultural chemicals is a focus of current people research and development.
Summary of the invention
The object of the present invention is to provide a kind of purple stem boneset extract and the composite bactericide of Metalaxyl-M and preventing and treating the application in plant mycosis.
Purple stem boneset extract and the composite bactericide of Metalaxyl-M, the active component of described bactericide is Metalaxyl-M and Herba Lycopi's ketone.
Described Herba Lycopi's ketone is 2-deoxidation-2-acetoxyl group-9-carbonyl Herba Lycopi ketone, 9-carbonyl-10H β Herba Lycopi ketone, one or more in 9-carbonyl-10H α Herba Lycopi's ketone and 9-carbonyl-10,11 dehydrogenation Herba Lycopi ketone.
In described bactericide, the concentration of Herba Lycopi's ketone is 5-300mg/L, and the concentration of Metalaxyl-M is 0.1-300mg/L, and all the other are acceptable carrier on water and agricultural chemicals.
On described agricultural chemicals, acceptable carrier is toluene, ethanol, dimethylbenzene, dimethyl sulfoxide (DMSO), tween, one or more in polyoxyethylene sorbitan carboxylic ester
The application in plant mycosis prevented and treated by above-mentioned purple stem boneset extract and the composite bactericide of Metalaxyl-M.
Described mycosis is Phytophthora capsici and P. myriotylum.
Compared with prior art, the present invention has following beneficial effect: composite bactericide of the present invention adopt natural plant extracts Herba Lycopi's ketone and Metalaxyl-M composite, decrease the usage amount of traditional agricultural chemicals Metalaxyl-M, meanwhile, add result of use, composite bactericide is a kind of efficient, low toxicity, degradation speed is fast, bactericide easy to use, effectively can prevent and treat Phytophthora capsici, the plant mycosises such as P. myriotylum.
Accompanying drawing explanation
Fig. 1 is the molecular structural formula of four kinds of Herba Lycopi's ketonic compounds.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in detail, but is to be understood that protection scope of the present invention not by the restriction of embodiment.
embodiment 1the extraction of purple stem boneset extract
Gather after Eupatorium adenophorum blade dries in the shade and be ground into meal, add the methyl alcohol of 6 times of meal weight, the ultrasonic extraction of room temperature three times, each 12h, merging filtrate, 45 DEG C of reduced pressure concentrations, obtain medicinal extract.Add a small amount of methyl alcohol in medicinal extract to dissolve, add distilled water and make methanol content be diluted to 20%, extraction into ethyl acetate, extract 45 DEG C of decompression and solvent recoveries, to dry, obtain acetic acid ethyl ester extract.Acetic acid ethyl ester extract is dissolved in mixed solution methyl alcohol: water: chloroform (85:15:5), upper XAD-2 macroporous resin column wash-out, removes the impurity such as pigment, collects eluant, eluent, concentrates to obtain paste.Paste after decolouring adopts carrene again: ethyl acetate 98:2-98:5 solvent system carries out silica gel column chromatography repeatedly, obtain purple stem boneset extract, extract is mainly containing Z6:2-deoxidation-2-acetoxyl group-9-carbonyl Herba Lycopi ketone, Z7:9-carbonyl-10H β Herba Lycopi ketone, Z8:9-carbonyl-10H α Herba Lycopi ketone, Z9:9-carbonyl-10,11 dehydrogenation Herba Lycopi ketone.The molecular structural formula of four kinds of Herba Lycopi's ketonic compounds is shown in Fig. 1.
embodiment 2 bacteriostatic experiment
For examination bacterium: Phytophthora capsici, P. myriotylum.
Reagent agent: Metalaxyl-M (Syngenta Co., Ltd), Herba Lycopi's ketone is prepared by the method for embodiment 1.
Purple stem boneset extract and the combined bactericide containing purple stem boneset extract adopt mycelial growth rate method to carry out in vitro biological activity determination to for examination pathogen.By following formulae discovery bacteriostasis rate: colony growth diameter (mm)=measure diameter mean value-4.0 (bacterium cake diameter) twice; The diameter X100% of Developing restraint rate=(diameter of the diameter-process bacterium colony of contrast bacterium colony)/contrast bacterium colony.
According to Colby method (1966) formulae discovery preventive effect and with actual measurement field efficacy, easy, effectively to evaluate mixture synergy effect.Computing formula is:
E＝X1×X2…×Xn/100(n-1)
E is the theoretical preventive effect of mixture; N is the quantity of mixed medicament; X1 represents the preventive effect after using the 1st kind of medicament; X2 represents the preventive effect after using the 2nd kind of pharmaceutical agent; Xn represents the preventive effect after using n-th kind of medicament.When the actual preventive effect of mixture is greater than theoretical preventive effect, represent synergy; When the actual preventive effect of mixture is less than theoretical preventive effect, represent antagonism.Experimental result is as shown in table 1-2.
Table 1 purple stem boneset extract control efficiency to Phytophthora capsici mixed with Metalaxyl-M
Table 2 purple stem boneset extract control efficiency to P. myriotylum mixed with Metalaxyl-M
From the result of table 1 and table 2, Metalaxyl-M is mixed compared with single dose with Herba Lycopi's ketone, all there is the effect of stronger Synergistic, for the control efficiency of Phytophthora capsici, the actual preventive effect of Herba Lycopi's ketone of 62.5mg/L Metalaxyl-M and 250mg/L reaches 100%, for the control of P. myriotylum, the Metalaxyl-M of 0.2mg/L and the actual preventive effect of Herba Lycopi's ketone of 40mg/L is used to reach 100%, selecting of this combination preparation, greatly reduces the usage amount of Metalaxyl-M, and adds result of use.
the control field experiment of embodiment 3 pairs of Phytophthora capsicis
Prepare medicament composition 1, containing 62.5mg/L Metalaxyl-M in composition 1,250mg/L Herba Lycopi's ketone, 50mg/L dimethylbenzene and 50mg/L dimethyl sulfoxide (DMSO), all the other are water.
Prepare medicament composition 2, containing 62.5mg/L Metalaxyl-M in composition 2,250mg/L Herba Lycopi's ketone, 50mg/L dimethylbenzene and 50mg/L polyoxyethylene sorbitan carboxylic ester, all the other are water.
Get the capsicum ground 200m of plantation
2infect Phytophthora capsici intercurrent disease, grade and moderate infection, is divided into two pieces, uses the agricultural chemicals of medicament composition 1 and medicament composition 2 respectively, each sprinkling 1L, sprayed once every one day, spray 3 times, after 15 days, the capsicum recovery rate using medicament composition 1 is 95%, and the capsicum recovery rate using medicament composition 2 is 99%.
the control field experiment of embodiment 4 pairs of P. myriotylum
Prepare medicament composition 1, containing 0.2mg/L Metalaxyl-M in composition 1,40mg/L Herba Lycopi's ketone, 50mg/L dimethylbenzene and 50mg/L dimethyl sulfoxide (DMSO), all the other are water.
Prepare medicament composition 2, containing 0.2mg/L Metalaxyl-M in composition 2,40mg/L Herba Lycopi's ketone, 50mg/L dimethylbenzene and 50mg/L polyoxyethylene sorbitan carboxylic ester, all the other are water.
Get the peanut ground 200m of plantation
2infect P. myriotylum intercurrent disease, grade and moderate infection, is divided into two pieces, uses the agricultural chemicals of medicament composition 1 and medicament composition 2 respectively, each pouring 1L (1L agricultural chemicals is watered in 1000L water and waters), every pouring in a day once, water 3 times, after 15 days, the peanut recovery rate using medicament composition 1 is 94%, and the peanut recovery rate using medicament composition 2 is 99%.
Be only specific embodiments of the invention above, but the present invention is not limited thereto, the changes that any person skilled in the art can think of all should fall into protection scope of the present invention.
Claims (6)
1. purple stem boneset extract and the composite bactericide of Metalaxyl-M, it is characterized in that, the active component of described bactericide is Metalaxyl-M and Herba Lycopi's ketone.
2. purple stem boneset extract according to claim 1 and the composite bactericide of Metalaxyl-M, it is characterized in that, described Herba Lycopi's ketone is 2-deoxidation-2-acetoxyl group-9-carbonyl Herba Lycopi ketone, 9-carbonyl-10H β Herba Lycopi ketone, one or more in 9-carbonyl-10H α Herba Lycopi's ketone and 9-carbonyl-10,11 dehydrogenation Herba Lycopi ketone.
3. purple stem boneset extract according to claim 1 and the composite bactericide of Metalaxyl-M, it is characterized in that, in described bactericide, the concentration of Herba Lycopi's ketone is 5-300mg/L, and the concentration of Metalaxyl-M is 0.1-300mg/L, and all the other are acceptable carrier on water and agricultural chemicals.
4. purple stem boneset extract according to claim 3 and the composite bactericide of Metalaxyl-M, it is characterized in that, on described agricultural chemicals, acceptable carrier is toluene, ethanol, dimethylbenzene, dimethyl sulfoxide (DMSO), tween, one or more in polyoxyethylene sorbitan carboxylic ester.
5. the application in plant mycosis prevented and treated by purple stem boneset extract according to claim 1 and the composite bactericide of Metalaxyl-M.
6. purple stem boneset extract according to claim 5 and the composite bactericide of Metalaxyl-M are preventing and treating the application in plant mycosis, and it is characterized in that, described mycosis is Phytophthora capsici and P. myriotylum.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510536151.3A CN105165830A (en) | 2015-08-27 | 2015-08-27 | Bactericide compounded by eupatorium adenophorum extract and mefenoxam |
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| CN201510536151.3A CN105165830A (en) | 2015-08-27 | 2015-08-27 | Bactericide compounded by eupatorium adenophorum extract and mefenoxam |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532707A (en) * | 2016-01-18 | 2016-05-04 | 中国农业科学院植物保护研究所 | Crofton weed extract and iprodione compound fungicide |
| CN115894201A (en) * | 2022-09-19 | 2023-04-04 | 扬州大学 | Extraction and separation method and application of eupatorium adenophorum spreng diketone |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104839153A (en) * | 2015-05-12 | 2015-08-19 | 乐山师范学院 | Natural insecticide for prevention and treatment of pseudoregma bambusicola |
-
2015
- 2015-08-27 CN CN201510536151.3A patent/CN105165830A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104839153A (en) * | 2015-05-12 | 2015-08-19 | 乐山师范学院 | Natural insecticide for prevention and treatment of pseudoregma bambusicola |
Non-Patent Citations (2)
| Title |
|---|
| 夏泽敏: "紫茎泽兰化学成分的分离鉴定及抑菌活性测定", 《广东农业科学》 * |
| 杨明秀: "精甲霜灵对大豆根腐病菌的毒力研究", 《东北农业大学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532707A (en) * | 2016-01-18 | 2016-05-04 | 中国农业科学院植物保护研究所 | Crofton weed extract and iprodione compound fungicide |
| CN115894201A (en) * | 2022-09-19 | 2023-04-04 | 扬州大学 | Extraction and separation method and application of eupatorium adenophorum spreng diketone |
| CN115894201B (en) * | 2022-09-19 | 2024-04-19 | 扬州大学 | Extraction and separation method and application of eupatorium adenophorum diketone in eupatorium adenophorum |
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Application publication date: 20151223 |