CN105153098A - Method for extracting procyanidins (OPC) from sorghum testa - Google Patents
Method for extracting procyanidins (OPC) from sorghum testa Download PDFInfo
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- CN105153098A CN105153098A CN201510667895.9A CN201510667895A CN105153098A CN 105153098 A CN105153098 A CN 105153098A CN 201510667895 A CN201510667895 A CN 201510667895A CN 105153098 A CN105153098 A CN 105153098A
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- chinese sorghum
- exosper
- powder
- solution
- enzymolysis
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
Abstract
The invention provides a method for extracting procyanidins (OPC) from sorghum testa. The OPC is extracted by using the sorghum testa as the main raw materials and combining an enzymic preparation with a solvent. The preparation method comprises the following steps: material preparation, buffer solution preparation, enzyme liquid preparation, feeding, enzymolysis, extraction and finished product preparation. The obtained OPC product can be applied to the industries of food additives-natural pigments, medical health care products and cosmetics with anti-oxidation effects, and the like.
Description
Technical field
The invention belongs to natural product processing scientific domain, particularly a kind of method extracting pycnogenols from Chinese sorghum exosper.
Background technology
Be otherwise known as pycnogenols (Procyanidins is called for short OPC) oligomeric flavanone, is a kind of new and effective antioxidant, is the most potent found so far free-radical scavengers, has very strong activity in vivo.Experiment proves, the Green Tea Extract oxidation capacity of OPC is 50 times of vitamin-E, ascorbic 20 times, and absorbs rapidly completely, and within oral 20 minutes, can reach the highest haemoconcentration, metabolic half life is for 7 hours.
Pycnogenols is kind the abundantest in nature phenolic compound, and pycnogenols produces coloured anthocyanidin because there is typical oxidation DeR under acid medium and gains the name.In all kinds of pycnogenols, dimer is the class pycnogenols that is the widest, most study that distributes.Report both at home and abroad a lot about the pharmacotoxicological effect of pycnogenols, the functions such as such as scavenging free radicals, strong anti-oxidation, antitumor and arteriosclerosis; Pycnogenols has also been used for the complication after preventing Operation of Cataract in Diabetics; In addition pycnogenols also serves vital role in plant defense predator and microbial mechanism.So act on widely in view of pycnogenols has, its extracting method also receives the very big concern of domestic and international researchist.
Found the multiple resource being rich in pycnogenols now, wherein especially with the most deep to the research of the pycnogenols in Pericarpium Vitis viniferae, seed, the most extensively, the most successful, achieve breakthrough progress.And to the rare bibliographical information of the research of the pycnogenols in Chinese sorghum exosper.
China is vast in territory, and the cultivated area of Chinese sorghum and output are all at the forefront in the world, is rich in pycnogenols in Chinese sorghum exosper, and this is rich in pycnogenols resource and is not used effectively.Modern biology and pharmaceutical research show, pycnogenols has a lot of physiologically active and pharmacologically active.As pycnogenols there is very strong resistance of oxidation, can remove various free radical and antitumor, delay senility, the multiple pharmacologically active such as anti-inflammatory, now be applied at clinical medicine and filed of daily-use chemical industry, toxicologic study shows that pycnogenols is without any toxic side effect simultaneously.
Utilize Chinese sorghum exosper to extract pycnogenols, be conducive to the added value improving this farm crop of Chinese sorghum, for the further investigation and application of pycnogenols is supplied raw materials, thus there is economic benefit and social benefit widely.
Summary of the invention
The object of the invention is the deficiency overcoming existing extracting method, propose a kind of method extracting pycnogenols from Chinese sorghum exosper.The pycnogenols product that this method obtains can be used for the industry such as medicines and health protection and makeup.
A kind of method extracting pycnogenols from Chinese sorghum exosper of the present invention, raw material is Chinese sorghum exosper, and reagent has polygalacturonase (30000IU), cellulase (15000IU), 95% ethanol (food grade), citric acid (food grade), Sodium phosphate dibasic (food grade).
A kind of method extracting pycnogenols from Chinese sorghum exosper of the present invention, is made up of following steps:
(1) get the raw materials ready: Chinese sorghum is carried out constant temperature drying under reduced pressure, pulverizing of milling, 40 orders sieve, and obtain Chinese sorghum exosper powder for subsequent use;
(2) buffered soln is prepared: mixed with the volume ratio of 0.2mol/L disodium phosphate soln by 1:1.5 ~ 2 by 0.1mol/L citric acid solution, then thin up 2 times, be mixed with buffered soln:
(3) prepare enzyme liquid: mixing buffered soln, polygalacturonase and cellulase, and fully stirring makes it dissolve completely, thus form uniform enzyme liquid; The consumption of buffered soln is in Chinese sorghum exosper powder weight (kg): the ratio calculating polygalacturonase of volume of buffer solution (L)=1:15 ~ 30 and the consumption of cellulase are that the buffered soln of every L adds 0.1 ~ 0:3g polygalacturonase and 0.1 ~ 0.3g cellulase;
(4) feed intake: in Chinese sorghum exosper powder weight (kg): the ratio of volume of buffer solution (L)=1:15 ~ 30, Chinese sorghum exosper powder is added in enzyme liquid;
(5) enzymolysis: after Chinese sorghum exosper powder and enzyme liquid are stirred, lucifuge insulation in the thermostat water bath of 50 ~ 55 DEG C, every 30min stirs once, enzymolysis 3-4h, generates the mixture of enzymolysis solution and residue;
(6) extract: 75 ~ 95% ethanol are added in above-mentioned enzymolysis solution and residue mixture by the method adding 75 ~ 95% ethanol of 15 ~ 30L by every kg Chinese sorghum exosper powder, under 60 ~ 65 DEG C of conditions, extract 2 ~ 3h, after filtration, obtain extracting solution;
(7) finished product: putting extracting solution in the baking oven of 60 ~ 70 DEG C dries to constant weight, and obtains pycnogenols.
A kind of method extracting pycnogenols from Chinese sorghum exosper of the present invention, with Chinese sorghum exosper for main raw material, adopt zymin in conjunction with solvent extraction pycnogenols, its advantage is that former blue and white plain Product Safety is good, quality is good, and former blue and white plain extraction yield comparatively alcohol extracting is followed the example of high by 30 ~ 40%, and the consumption of zymin is few, and technique is simple, easy to operate, efficiency is high, is applicable to suitability for industrialized production.The pectin substance using polygalacturonase and cellulase can destroy cell wall structure based on Mierocrystalline cellulose in plant material and iuntercellular thereof to be connected, makes extraction resistance to mass transfer reduce, makes the former blue and white plain composition of content be easy to stripping.Ethanolic soln is as the Extraction solvent of the former blue and white element of alcohol dissolubility.Citric acid phosphoric acid buffered soln plays the effect of regulator solution pH value.By the buffered soln that method of the present invention is prepared, its pH=6 after testing, can ensure the best effect of zymin effect.
Specific embodiment: in order to fully disclose a kind of method extracting pycnogenols from Chinese sorghum exosper of the present invention, be illustrated below in conjunction with embodiment.
Embodiment: a kind of method extracting pycnogenols from Chinese sorghum exosper, its method comprises the following steps:
L () sets high fine strain of millet and dries in 65 DEG C of baking ovens, pulverize, within 40th, sieve, obtain Chinese sorghum exosper powder for subsequent use with high-speed tissue mashing machine.
(2) get 0.1mol/L citric acid solution 160ml, 0.2mol/L disodium phosphate soln 320ml, mix and dilute and be settled to 1000ml, be mixed with buffered soln.
(3) get the buffered soln of 250ml, add 0.05g polygalacturonase and 0.075g cellulase, stir and make it dissolve completely, form uniform enzyme liquid.
(4) in above-mentioned enzyme liquid, add the Chinese sorghum exosper powder of 10g.
(5) lucifuge insulation in the thermostat water bath of 50 DEG C after stirring, every 30min stirs once, enzymolysis 3h, generates the mixture of enzymolysis solution and residue.
(6) in the mixture of enzymolysis solution and residue, add 250m195% ethanol, under 60 DEG C of conditions, extract 2h, after filtration extracting solution.
(7) putting extracting solution in the baking oven of 65 DEG C dries to constant weight, and obtains pycnogenols.
Claims (2)
1. extract a method for pycnogenols from Chinese sorghum exosper, it is characterized in that being made up of following steps:
(1) get the raw materials ready: Chinese sorghum is carried out constant temperature drying under reduced pressure, pulverizing of milling, 40 orders sieve, and obtain Chinese sorghum exosper powder for subsequent use;
(2) buffered soln is prepared: mixed with the volume ratio of 0.2mol/L disodium phosphate soln by 1:1.5 ~ 2 by 0.1mol/L citric acid solution, then thin up 2 times, be mixed with buffered soln;
(3) prepare enzyme liquid: mixing buffered soln, polygalacturonase and cellulase, and fully stirring makes it dissolve completely, thus form uniform enzyme liquid; The consumption of buffered soln is in Chinese sorghum exosper powder weight (kg): the ratio calculating polygalacturonase of volume of buffer solution (L)=1:15 ~ 30 and the consumption of cellulase are that the buffered soln of every L adds 0.1 ~ 0:3g polygalacturonase and 0.1 ~ 0.3g cellulase;
(4) feed intake: in Chinese sorghum exosper powder weight (kg): the ratio of volume of buffer solution (L)=1:15 ~ 30, Chinese sorghum exosper powder is added in enzyme liquid;
(5) enzymolysis: after Chinese sorghum exosper powder and enzyme liquid are stirred, lucifuge insulation in the thermostat water bath of 50 ~ 55 DEG C, every 30min stirs once, enzymolysis 3-4h, generates the mixture of enzymolysis solution and residue;
(6) extract: 75 ~ 95% ethanol are added in above-mentioned enzymolysis solution and residue mixture by the method adding 75 ~ 95% ethanol of 15 ~ 30L by every kg Chinese sorghum exosper powder, under 60 ~ 65 DEG C of conditions, extract 2 ~ 3h, after filtration, obtain extracting solution;
(7) finished product: putting extracting solution in the baking oven of 60 ~ 70 DEG C dries to constant weight.
2. a kind of method extracting pycnogenols from Chinese sorghum exosper according to claim 1, is characterized in that being made up of following steps:
(1) put fresh Chinese sorghum to dry in 65 DEG C of baking ovens, pulverize with high-speed tissue mashing machine, 40 orders sieve, and obtain Chinese sorghum exosper powder for subsequent use;
(2) get 0.lmol/L citric acid solution 160ml, 0.2mol/L disodium phosphate soln 320m1, mix and dilute and be settled to 1000ml, be mixed with buffered soln;
(3) get the buffered soln of 250ml, add 0.05g polygalacturonase and 0.075g cellulase, stir and make it dissolve completely, form uniform enzyme liquid;
(4) in above-mentioned enzyme liquid, add the Chinese sorghum exosper powder of 10g;
(5) lucifuge insulation in the thermostat water bath of 50 DEG C after stirring, every 30min stirs once, enzymolysis 3h, generates the mixture of enzymolysis solution and residue;
(6) in the mixture of enzymolysis solution and residue, add 250m195% ethanol, under 60 DEG C of conditions, extract 2h, after filtration extracting solution;
(7) putting extracting solution in the baking oven of 65 DEG C dries to constant weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510667895.9A CN105153098A (en) | 2015-10-16 | 2015-10-16 | Method for extracting procyanidins (OPC) from sorghum testa |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510667895.9A CN105153098A (en) | 2015-10-16 | 2015-10-16 | Method for extracting procyanidins (OPC) from sorghum testa |
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| CN105153098A true CN105153098A (en) | 2015-12-16 |
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| CN201510667895.9A Pending CN105153098A (en) | 2015-10-16 | 2015-10-16 | Method for extracting procyanidins (OPC) from sorghum testa |
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2015
- 2015-10-16 CN CN201510667895.9A patent/CN105153098A/en active Pending
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Application publication date: 20151216 |