CN105152695A - Biological fertilizer for increasing content of effective components of eucommia ulmoides and preparation method of biological fertilizer - Google Patents
Biological fertilizer for increasing content of effective components of eucommia ulmoides and preparation method of biological fertilizer Download PDFInfo
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- CN105152695A CN105152695A CN201510670995.7A CN201510670995A CN105152695A CN 105152695 A CN105152695 A CN 105152695A CN 201510670995 A CN201510670995 A CN 201510670995A CN 105152695 A CN105152695 A CN 105152695A
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- epipremnum aureum
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Abstract
The invention relates to a biological fertilizer for increasing the content of main effective components in eucommia ulmoides plants and a preparation method of the biological fertilizer. The biological fertilizer is prepared from the following raw materials in parts by weight: 2-5 parts of juice liquid of scindapsus aureus leaves, 3-5 parts of juice liquid of kidney bean pod, 3-7 parts of juice liquid of sunflower petals and 300 parts of water. The biological fertilizer is prepared from the juice liquid of the scindapsus aureus leaves, the juice liquid of kidney bean pod and the juice liquid of sunflower petals which are used as the raw materials by a series of processes such as fermentation; the fertilizer is safe and pollution-free; firstly, the biological fertilizer is capable of effectively increasing the content of comprehensive extractive of chlorogenic acid and pinoresinol diglucoside which are used as main effective components in the eucommia ulmoides; meanwhile, the diameter of the eucommia ulmoides trunk is also significantly increased; secondly, the transplanting survival rate of the eucommia ulmoides can be increased by applying the biological fertilizer in the seedling stage.
Description
Technical field
The present invention relates to technical field of fertilizers, particularly relate to a kind of bio-feritlizer and preparation method thereof improving main pharmacodynamics component content in bark of eucommia plantation.
Background technology
The bark of eucommia (EucommiaUlmoidesOliv.) is the distinctive economic tree of China, the chlorogenic acid wherein contained has healing wound, prevents the effects such as inflammation, Pinoresinol diglucoside has the effect of step-down, and its Hypotensive Mechanism directly acts on unstriated muscle, and peripheral blood vessel is expanded.Existing fertilizer great majority have certain pollution to environment, the content of bark of eucommia Content of Chlorogenic Acid and Pinoresinol diglucoside is not increased significantly, bio-feritlizer environmentally safe, but, the prior art raw material spent when preparing bio-feritlizer is too many, cumbersome, causes economic benefit not high.
Summary of the invention
Object of the present invention provides a kind of bio-feritlizer for improving main pharmacodynamics component content in bark of eucommia plantation and preparation method thereof.
Technical scheme of the present invention is as follows:
Bio-feritlizer is made up of the raw material of following weight part: a juice 3-7 part for juice 2-5 part of epipremnum aureum leaf, juice 3-5 part of kidney bean beanpod, Sunflower Receptacle petal, 300 parts, water.
Prepare a method for above-mentioned bio-feritlizer, concrete steps are as follows:
1, the leaf of epipremnum aureum is squeezed the juice, obtains epipremnum aureum leaf juice, for subsequent use; The beanpod of kidney bean is squeezed the juice, obtains kidney bean beanpod juice, for subsequent use; The petal of Sunflower Receptacle is squeezed the juice, obtains Sunflower Receptacle petal juice, for subsequent use.
2, by epipremnum aureum juice, kidney bean beanpod juice, the mixing of Sunflower Receptacle petal juice, add distilled water, load in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, and take out and be placed on shaking table, rotating speed is 120r/min, temperature is 35 DEG C, cultivates 30 days, has fermented and obtained this bio-feritlizer.
Compared with prior art, the present invention has following outstanding substantive distinguishing features and significant advantage:
The bio-feritlizer that the present invention obtains, epipremnum aureum leaf sap, kidney bean beanpod juice, Sunflower Receptacle petal juice are as raw material by fermentation and series of process and obtaining, fertilizer safety non-pollution, effectively can improve the content of the comprehensive extract of effective component chlorogenic acid main in the bark of eucommia and Pinoresinol diglucoside, also significantly increase the diameter of bark of eucommia trunk simultaneously thick, used this bio-feritlizer in seedling stage and can promote bark of eucommia transplanting survival rate.The fertilizer that the present invention makes not only has good promoter action, also for effectively to utilize epipremnum aureum leaf, kidney bean beanpod, Sunflower Receptacle petal to provide new thinking to the healthy growth of the bark of eucommia.
Embodiment
Epipremnum aureum, Classification system Epipremnumaureum, Araeceae epipremnum aureum belongs to large-scale evergreen vine, and what the present invention adopted is its leaf; Kidney bean, Chinese formal name used at school Kidney bean, Classification system PhaseolusvulgarisLinn, pulse family Phaseolus plant, what the present invention adopted is its beanpod; Sunflower Receptacle, Classification system Helianthusannuus, composite family Helianthus plant, what the present invention adopted is its petal.
Usedly in the present invention to squeeze the juice, sterilizing, main pharmacodynamics component content detection technique all belongs to prior art in the bark of eucommia, epipremnum aureum juice of the present invention, kidney bean beanpod juice, Sunflower Receptacle petal juice manufacture like this to obtain:
The leaf of epipremnum aureum is squeezed the juice, obtains epipremnum aureum leaf juice; The beanpod of kidney bean is squeezed the juice, obtains kidney bean beanpod juice; The petal of Sunflower Receptacle is squeezed the juice, obtains Sunflower Receptacle petal juice.Juice-extracting process carries out at normal temperatures.
By epipremnum aureum juice, kidney bean beanpod juice, the mixing of Sunflower Receptacle petal juice, add distilled water, load in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, take out and be placed on shaking table, rotating speed is 120r/min, temperature is 35 DEG C, cultivates 30 days, has fermented and obtained this bio-feritlizer.
Choose the bark of eucommia seedling that diameter is 4cm, within first 3 days, in each seedling hole, the above-mentioned obtained bio-feritlizer of 500ml is poured in transplanting, transplant and topdress for latter 1 day, 500ml bio-feritlizer is poured in each seedling hole, often group test repetition 6 times, each 50 strain barks of eucommia, namely often organize the test 300 strain bark of eucommia, except interpolation bio-feritlizer, bark of eucommia control measures adopt Routine Management, to the Routine Management of the plantation bark of eucommia, belong to prior art, within 20 days, check that bark of eucommia seedling survives situation and record afterwards, blade is unfolded, in green or peak green, and bright in color, branch is unfolded, and is and survives.Control group is executed and is only distilled water.After bark of eucommia transplantation of seedlings 60 days, the diameter of experiment with measuring group and the control group bark of eucommia respectively, chlorogenic acid is utilized to be dissolved in the character of ethanol, by alcohol extracting method chlorogenic acid extracting, with the separation method that repeatedly silica gel column chromatography combines, extraction purification is carried out to Pinoresinol diglucoside in bark of eucommia leaf with multistage organic solvent extraction, record test group and the chlorogenic acid of control group and the content of Pinoresinol diglucoside respectively.
Battery of tests
Fertilizer formula is obtained by following steps:
The first step, squeezes the juice the leaf of epipremnum aureum, obtains epipremnum aureum juice, for subsequent use; The beanpod of kidney bean is squeezed the juice, obtains kidney bean beanpod juice, for subsequent use; The petal of Sunflower Receptacle is squeezed the juice, obtains Sunflower Receptacle petal juice, for subsequent use,
Second step, by epipremnum aureum juice, kidney bean beanpod juice, the mixing of Sunflower Receptacle petal juice, add distilled water, load in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, and take out and be placed on shaking table, rotating speed is 120r/min, and temperature is 35 DEG C, cultivates 30 days, completes fermentation.
And: epipremnum aureum leaf juice weight part is 4, and the weight part of kidney bean beanpod juice is 2, and the weight part of Sunflower Receptacle petal juice is 5, and the weight part of water is 300.
The strain of test bark of eucommia quantity 300, quantity is survived: 291 strains after transplanting, mean diameter: 11cm, average chlorogenic acid content: 5.81mg/g (every gram of blade contains chlorogenic acid 5.81mg), average Pinoresinol diglucoside content: 4.15mg/g (every gram of blade contains Pinoresinol diglucoside 4.15mg)
Second group of test
Fertilizer formula is obtained by following steps:
The first step, squeezes the juice the leaf of epipremnum aureum, obtains epipremnum aureum juice, for subsequent use; The beanpod of kidney bean is squeezed the juice, obtains kidney bean beanpod juice, for subsequent use; The petal of Sunflower Receptacle is squeezed the juice, obtains Sunflower Receptacle petal juice, for subsequent use,
Second step, by epipremnum aureum juice, kidney bean beanpod juice, the mixing of Sunflower Receptacle petal juice, add distilled water, load in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, and take out and be placed on shaking table, rotating speed is 120r/min, and temperature is 35 DEG C, cultivates 30 days, completes fermentation.
And: epipremnum aureum leaf juice weight part is 3, and the weight part of kidney bean beanpod juice is 5, and the weight part of Sunflower Receptacle petal juice is 4, and the weight part of water is 300.
The strain of test bark of eucommia quantity 300, quantity is survived: 290 strains after transplanting, mean diameter: 10.2cm, average chlorogenic acid content: 5.72mg/g (every gram of blade contains chlorogenic acid 5.72mg), average Pinoresinol diglucoside content: 4.11mg/g (every gram of blade contains Pinoresinol diglucoside 4.11mg)
3rd group of test
Fertilizer formula is obtained by following steps:
The first step, squeezes the juice the leaf of epipremnum aureum, obtains epipremnum aureum juice, for subsequent use; The beanpod of kidney bean is squeezed the juice, obtains kidney bean beanpod juice, for subsequent use; The petal of Sunflower Receptacle is squeezed the juice, obtains Sunflower Receptacle petal juice, for subsequent use,
Second step, by epipremnum aureum juice, kidney bean beanpod juice, the mixing of Sunflower Receptacle petal juice, add distilled water, load in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, and take out and be placed on shaking table, rotating speed is 120r/min, and temperature is 35 DEG C, cultivates 30 days, completes fermentation.
And: epipremnum aureum leaf juice weight part is 6, and the weight part of kidney bean beanpod juice is 7, and the weight part of Sunflower Receptacle petal juice is 3, and the weight part of water is 300.
The strain of test bark of eucommia quantity 300, quantity is survived: 294 strains after transplanting, mean diameter: 12.3cm, average chlorogenic acid content: 5.81mg/g (every gram of blade contains chlorogenic acid 5.81mg), average Pinoresinol diglucoside content: 4.25mg/g (every gram of blade contains Pinoresinol diglucoside 4.25mg)
4th group of test
Control group is tested, and pours into and be only distilled water in seedling hole.
The strain of test bark of eucommia quantity 300, quantity is survived: 78 strains after transplanting, mean diameter: 6.3cm, average chlorogenic acid content: 2.14mg/g (every gram of blade contains chlorogenic acid 2.14mg), average Pinoresinol diglucoside content: 1.95mg/g (every gram of blade contains Pinoresinol diglucoside 1.95mg)
5th group of test
Fertilizer formula is obtained by following steps:
The first step, squeezes the juice the leaf of epipremnum aureum, obtains epipremnum aureum juice, for subsequent use,
Second step, mixes epipremnum aureum juice, adds distilled water, and load in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, and take out and be placed on shaking table, rotating speed is 120r/min, and temperature is 35 DEG C, cultivates 30 days, completes fermentation.
And: epipremnum aureum leaf juice weight part is 6, and the weight part of water is 300.
The strain of test bark of eucommia quantity 300, quantity is survived: 108 strains after transplanting, mean diameter: 6.7cm, average chlorogenic acid content: 3.02mg/g (every gram of blade contains chlorogenic acid 3.02mg), average Pinoresinol diglucoside content: 2.15mg/g (every gram of blade contains Pinoresinol diglucoside 2.15mg)
6th group of test
Fertilizer formula is obtained by following steps:
The first step, squeezes the juice the beanpod of kidney bean, obtains kidney bean beanpod juice, for subsequent use,
Second step, mixes kidney bean beanpod juice, adds distilled water, and load in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, and take out and be placed on shaking table, rotating speed is 120r/min, and temperature is 35 DEG C, cultivates 30 days, completes fermentation.
And: the weight part of kidney bean beanpod juice is 7, and the weight part of water is 300.
The strain of test bark of eucommia quantity 300, quantity is survived: 114 strains after transplanting, mean diameter: 7.1cm, average chlorogenic acid content: 3.06mg/g (every gram of blade contains chlorogenic acid 3.06mg), average Pinoresinol diglucoside content: 2.25mg/g (every gram of blade contains Pinoresinol diglucoside 2.25mg)
7th group of test
Fertilizer formula is obtained by following steps:
The first step, squeezes the juice the petal of Sunflower Receptacle, obtains Sunflower Receptacle petal juice, for subsequent use,
Second step, mixes Sunflower Receptacle petal juice, adds distilled water, and load in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, and take out and be placed on shaking table, rotating speed is 120r/min, and temperature is 35 DEG C, cultivates 30 days, completes fermentation.
And: the weight part of Sunflower Receptacle petal juice is 3, and the weight part of water is 300.
The strain of test bark of eucommia quantity 300, quantity is survived: 123 strains after transplanting, mean diameter: 7.9cm, average chlorogenic acid content: 3.12mg/g (every gram of blade contains chlorogenic acid 3.12mg), average Pinoresinol diglucoside content: 2.33mg/g (every gram of blade contains Pinoresinol diglucoside 2.23mg)
8th group of test
Fertilizer formula is obtained by following steps:
The first step, squeezes the juice the leaf of epipremnum aureum, obtains epipremnum aureum juice, for subsequent use; The beanpod of kidney bean is squeezed the juice, obtains kidney bean beanpod juice, for subsequent use,
Second step, by epipremnum aureum juice, the mixing of kidney bean beanpod juice, add distilled water, load in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, and take out and be placed on shaking table, rotating speed is 120r/min, and temperature is 35 DEG C, cultivates 30 days, completes fermentation.
And: epipremnum aureum leaf juice weight part is 6, and the weight part of kidney bean beanpod juice is 7, and the weight part of water is 300.
The strain of test bark of eucommia quantity 300, quantity is survived: 172 strains after transplanting, mean diameter: 8.1cm, average chlorogenic acid content: 3.32mg/g (every gram of blade contains chlorogenic acid 3.32mg), average Pinoresinol diglucoside content: 3.07mg/g (every gram of blade contains Pinoresinol diglucoside 3.07mg)
9th group of test
Fertilizer formula is obtained by following steps:
The first step, squeezes the juice the leaf of epipremnum aureum, obtains epipremnum aureum juice, for subsequent use; The petal of Sunflower Receptacle is squeezed the juice, obtains Sunflower Receptacle petal juice, for subsequent use,
Second step, by epipremnum aureum juice, the mixing of Sunflower Receptacle petal juice, add distilled water, load in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, and take out and be placed on shaking table, rotating speed is 120r/min, and temperature is 35 DEG C, cultivates 30 days, completes fermentation.
And: epipremnum aureum leaf juice weight part is 6, and the weight part of Sunflower Receptacle petal juice is 3, and the weight part of water is 300.
The strain of test bark of eucommia quantity 300, quantity is survived: 178 strains after transplanting, mean diameter: 8.4cm, average chlorogenic acid content: 3.41mg/g (every gram of blade contains chlorogenic acid 3.41mg), average Pinoresinol diglucoside content: 2.89mg/g (every gram of blade contains Pinoresinol diglucoside 2.89mg)
Tenth group of test
Fertilizer formula is obtained by following steps:
The first step, squeezes the juice the beanpod of kidney bean, obtains kidney bean beanpod juice, for subsequent use; The petal of Sunflower Receptacle is squeezed the juice, obtains Sunflower Receptacle petal juice, for subsequent use,
Second step, by kidney bean beanpod juice, the mixing of Sunflower Receptacle petal juice, add distilled water, load in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, and take out and be placed on shaking table, rotating speed is 120r/min, and temperature is 35 DEG C, cultivates 30 days, completes fermentation.
And: the weight part of kidney bean beanpod juice is 7, and the weight part of Sunflower Receptacle petal juice is 3, and the weight part of water is 300.
The strain of test bark of eucommia quantity 300, quantity is survived: 171 strains after transplanting, mean diameter: 8.5cm, average chlorogenic acid content: 3.44mg/g (every gram of blade contains chlorogenic acid 3.44mg), average Pinoresinol diglucoside content: 2.77mg/g (every gram of blade contains Pinoresinol diglucoside 2.77mg).
Claims (2)
1. for improving the bio-feritlizer of bark of eucommia content of drug effect components, it is characterized in that: described fertilizer is obtained by following steps:
The first step, squeezes the juice the leaf of epipremnum aureum, obtains epipremnum aureum juice, for subsequent use; The beanpod of kidney bean is squeezed the juice, obtains kidney bean beanpod juice, for subsequent use; The petal of Sunflower Receptacle is squeezed the juice, obtains Sunflower Receptacle petal juice, for subsequent use,
Second step, by epipremnum aureum juice, kidney bean beanpod juice, the mixing of Sunflower Receptacle petal juice, adds distilled water, loads in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, and take out and be placed on shaking table, rotating speed is 120r/min, temperature is 35 DEG C, cultivates 30 days, completes and ferment
And: epipremnum aureum leaf juice weight part is 4, and the weight part of kidney bean beanpod juice is 2, and the weight part of Sunflower Receptacle petal juice is 5.
2., for improving the manufacture method of the bio-feritlizer of bark of eucommia content of drug effect components, it is characterized in that: said method comprising the steps of:
The first step, squeezes the juice the leaf of epipremnum aureum, obtains epipremnum aureum juice, for subsequent use; The beanpod of kidney bean is squeezed the juice, obtains kidney bean beanpod juice, for subsequent use; The petal of Sunflower Receptacle is squeezed the juice, obtains Sunflower Receptacle petal juice, for subsequent use,
Second step, by epipremnum aureum juice, kidney bean beanpod juice, the mixing of Sunflower Receptacle petal juice, adds distilled water, loads in wide-necked bottle, sealed membrane seals, 120 DEG C of sterilizing 30min, and take out and be placed on shaking table, rotating speed is 120r/min, temperature is 35 DEG C, cultivates 30 days, completes fermentation and get final product.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102898241A (en) * | 2012-10-30 | 2013-01-30 | 湖州康寿生物科技有限公司 | Vegetable fertilizer and manufacture method of vegetable fertilizer |
| CN103058742A (en) * | 2013-01-30 | 2013-04-24 | 王代举 | Natural plant fertilizer and preparation method thereof |
| CN103964964A (en) * | 2014-05-23 | 2014-08-06 | 贵州三仁堂药业有限公司 | Special fertilizer for eucommia forest, and preparation method and application method thereof |
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- 2015-10-01 CN CN201510670995.7A patent/CN105152695A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102898241A (en) * | 2012-10-30 | 2013-01-30 | 湖州康寿生物科技有限公司 | Vegetable fertilizer and manufacture method of vegetable fertilizer |
| CN103058742A (en) * | 2013-01-30 | 2013-04-24 | 王代举 | Natural plant fertilizer and preparation method thereof |
| CN103964964A (en) * | 2014-05-23 | 2014-08-06 | 贵州三仁堂药业有限公司 | Special fertilizer for eucommia forest, and preparation method and application method thereof |
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