CN105145423B - A kind of method in quick discrimination river crab difference husking period - Google Patents
A kind of method in quick discrimination river crab difference husking period Download PDFInfo
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- CN105145423B CN105145423B CN201510460121.9A CN201510460121A CN105145423B CN 105145423 B CN105145423 B CN 105145423B CN 201510460121 A CN201510460121 A CN 201510460121A CN 105145423 B CN105145423 B CN 105145423B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
A kind of method in quick discrimination river crab difference husking period, it is characterized in that it is the following steps are included: be first turned on color difference meter, the correction of auto black and white plate is carried out first with blank, after correction, into canonical measure, read mode is adjusted to Lab mode, wherein, L value indicates the lightness of color ,+indicate partially white ,-indicate partially dark；A value indicates the green red value of color ,+indicate partially red ,-indicate partially green；B value indicates the yellow value of the indigo plant of color ,+indicate partially yellow ,-indicate partially blue；River crab individual is then taken out, its carapace back is dried, the blue yellow value (b) of color for reading river crab carapace ectoskeleton with color difference meter；Finally determine according to blue yellow value (b) work of the color of reading is following: as b >=7, individual is in proecdysis；As b≤2, individual is in postmolt；As 2 < b < 7 of b value, individual is in intermolt.The method of the present invention is simple, easy to operate, and detection will not be caused to damage to river crab, provides foundation to improve river crab raising and management level.
Description
Technical field
The present invention relates to a kind of aquaculture technology, especially a kind of culture of Chinese mitten crab technology is specifically a kind of quick
Differentiate the method in river crab difference husking period.
Background technique
The history of life of river crab is made of multiple moulting cycle process.Each moulting cycle include proecdysis (D),
Ecdysis (E), postmolt (A/B), intermolt (C).Proecdysis (D) hormone in vivo Secretion regulation is active, in blood
Moulting hormone level rises, and endepidermis starts gradually to separate with ectoskeleton.The rupture of ecdysis (E) ectoskeleton, crab body is from old shell
It sloughs off out.Postmolt (A/B phase) crab body largely absorbs moisture, and body is long, weight quicklys increase.Intermolt (C) is body weight gain
It is not further added by, nutriment starts to accumulate, and completing tissue growth is that husking growth next time carries out substance and energy prepares.In river crab
In cultivation growth and process of experimental, how fast and accurately to differentiate and divide moulting cycle period locating for crab body, for river
Crab cultivation husking peak period determination and soft crab production etc. have important theory significance and application value.
One, the importance of river crab husking
1, the growth of river crab is achieved by periodically husking, and each molting process requires to slough off old epidermis, closes
At new epidermis, while Individual Size and weight realize that a great-leap-forward increases within a short period of time.
2, river crab moulting cycle includes proecdysis (D), ecdysis (E), postmolt (A/B), intermolt (C).It sloughs off
Skin early period (D) hormone in vivo Secretion regulation is active, and the moulting hormone level in blood rises, and endepidermis and ectoskeleton start gradually
Separation.The rupture of ecdysis (E) ectoskeleton, crab body are sloughed off out from old shell.Postmolt (A/B phase) crab body largely absorbs moisture, body
Long, weight quicklys increase.Intermolt (C) is that body weight gain is not further added by, and nutriment starts to accumulate, and completes tissue growth and is
Husking growth next time carries out substance and energy prepares.
3, husking runs through the river crab entire history of life, and in periodical molting process, carapace absorbs a large amount of water after husking
Point, the wide rapid amplification of the long body of body directly decides growth rate；Husking generating process need to consume big energy, while lose movement
With defence capability, survival rate is directly influenced；Last time reproduction husking and sexal maturity are closely coupled, and reproduction husking in advance will
It will lead to the generation of sex premature phenomenon, therefore husking is the key that determine river crab growth rate and survival rate.
Two, the status that river crab moulting cycle differentiates
It is divided at present about the differentiation of river crab moulting cycle:
1, in laboratory conditions, mainly by way of microscope inspection, it is with the bristle generating process of maxilla
Foundation divides the progress of Eriocheir sinensis moulting cycle by stages, although the method for discrimination in laboratory is accurate, but a disadvantage is that needing
The professional equipments such as microscope are wanted, and the maxilla for winning river crab body easily leads to individual death.
2, poultry feeders of the division of river crab moulting cycle mainly with certain cultivation experience during breeding production
According to personal experience, rough differentiation is carried out in conjunction with the morphological features such as crust color and hardness, this method is taking human as experience
Based on, differentiate not accurate enough, shortage quantitative criteria.
Summary of the invention
The purpose of the present invention is the laboratory methods used in dividing for existing river crab ecdysis to need patent equipment,
At high cost, and the problem of artificial cognition method accuracy difference, inventing a kind of can be realized ecdysis using simple device and quickly determines
To provide the method that the quick discrimination river crab difference of foundation casts off a skin period to raise decision.
The technical scheme is that
A kind of method in quick discrimination river crab difference husking period, it is characterized in that it the following steps are included:
It is first turned on color difference meter, carries out the correction of auto black and white plate first with blank, after correction, into canonical measure,
Read mode is adjusted to Lab mode, wherein L value indicates the lightness of color ,+indicate partially white ,-indicate partially dark；A value indicates face
The green red value of color ,+indicate partially red ,-indicate partially green；B value indicates the yellow value of the indigo plant of color ,+indicate partially yellow ,-indicate partially blue；
River crab individual is then taken out, its carapace back is dried, the face of river crab carapace ectoskeleton is read with color difference meter
The blue yellow value (b) of color；
Finally determine according to blue yellow value (b) work of the color of reading is following: as b >=7, individual is in proecdysis；When b≤
When 2, individual is in postmolt；As 2 < b < 7 of b value, individual is in intermolt.
The color difference meter is portable colorimeter.
The good place of daylighting indoors should be selected to carry out when measurement.
The taking-up river crab individual, its carapace back is dried and is carried out at low ambient temperatures, to reduce river crab
Vigor, convenient for measurement.
The low temperature environment, which refers to, is placed in environment temperature 3 points under 0-4 degrees celsius for the river crab individual of taking-up
More than clock its carapace is dried again.
Beneficial effects of the present invention:
1, in laboratory, the present invention is more more convenient than laboratory microscopy method, and workload is small, and will not damage river crab
Individual influences growth, this makes in the case where not damaging river crab individual condition, divides different moulting cycle river crabs, carries out related experiment and grinds
Study carefully and is possibly realized.
2, using method of the invention, the quick discrimination river crab period can be predicted to judge that river crab sloughs off during breeding production
Skin peak period reduces the death rate to instruct the production of husking peak period, improves growth rate.
(1) death rate is reduced.Since husking peak period river crab individual is extremely fragile, river crab death will be will lead to using drug
Rate increases, and after differentiating husking peak period by quick discrimination method, drug use can be stopped in advance, to reduce death
Rate.
(2) growth rate is improved.The river crab husking growth course stage needs a large amount of calcium ions and magnesium ions, therefore in husking peak period bait
Material feeds element needed for middle addition calcium and magnesium etc. is casted off a skin, and husking can be promoted to grow, and improves growth rate.
3, it creates conditions for production soft crab.Shell-fish is due to tasting cumbersome and making some with hard carapace
It hangs back.It is temporary that the river crab individual that will be casted off a skin can be carried out by Fast Classification using quick discrimination river crab moulting cycle method
It supports, soft crab is collected after the completion of casting off a skin, is processed, to create conditions for production soft crab.
4, the present invention is convenient and efficient, convenient for operation, does not need laboratory profession instrument and equipment, and will not make to river crab individual
At damage；It is more accurate compared with the differentiation of the experience of breeding production reliable, there is operability and repeatability, even if not cultivating warp
The personnel tested can also carry out quick discrimination.
Specific embodiment
Below with reference to embodiment, the present invention is further illustrated.
A kind of method in quick discrimination river crab difference husking period, principle is existed based on carapace endepidermis and ectoskeleton
The tightness degree that different husking periods combine reads different husking periods to show different color differences, using color difference meter
Color data, thus differentiate divide individual locating for the moulting cycle stage.The present invention is according to river crab difference husking period cephalothorax
First color difference establishes the color data range in river crab difference husking period, reads different husking period individuals using color difference meter
Color data, compare color data, determine divide individual the moulting cycle stage, have it is convenient and efficient, convenient for operation, and have
There is higher accuracy, is of great significance to river crab healthy aquaculture and soft crab production.Specific step is as follows:
It is first turned on color difference meter (portable colorimeter can be used), carries out the correction of auto black and white plate, correction first with blank
After, into canonical measure, read mode is adjusted to Lab mode, wherein L value indicates the lightness of color ,+indicate inclined
It is white ,-indicate partially dark；A value indicates the green red value of color ,+indicate partially red ,-indicate partially green；B value indicates the yellow value of the indigo plant of color ,+table
Show partially yellow ,-expression indigo plant partially；
River crab individual is then taken out, is placed at least 3 minutes for (0-4 degrees Celsius) at low ambient temperatures, to reduce river crab
Mobility is more convenient for measuring, then its carapace back is dried, and the color for reading river crab carapace ectoskeleton with color difference meter is blue
Huang value (b)；Ice chest can be used in the low temperature environment or low temperature chamber carries out；
Finally determine according to blue yellow value (b) work of the color of reading is following: as b >=7, individual is in proecdysis；When b≤
When 2, individual is in postmolt；As 2 < b < 7 of b value, individual is in intermolt.
What color difference meter of the invention was read is the color of river crab carapace shell i.e. ectoskeleton, the difference of this color
Variation is being tightly combined degree and showing between one layer of endepidermis below shell (ectoskeleton) and shell due to river crab
The color difference come.
In order to verify accuracy of the invention, applicant first passes through microscope detection and has been determined 15 batches totally 45 river crabs are not
With moulting cycle period, while the b value of 15 batches of totally 45 river crabs is had recorded using portable colorimeter, is shown in Table 1.It can be with from table 1
Find out range locating for the targeted different husking period river crab carapace ectoskeleton color values of method of the invention, meets statistics
Rule is learned, there is directive significance.
The color difference b value range in 1 river crab difference of table husking period
| Serial number | Proecdysis (D) b value | Postmolt (A/B) b value | Intermolt (C) b value |
| 1 | 7.22 | 1.23 | 5.20 |
| 2 | 7.89 | 2.43 | 4.20 |
| 3 | 7.75 | 1.38 | 4.98 |
| 4 | 8.43 | 0.58 | 2.67 |
| 5 | 10.82 | 0.67 | 2.40 |
| 6 | 9.62 | 0.84 | 4.08 |
| 7 | 8.25 | 2.10 | 2.32 |
| 8 | 10.58 | 2.45 | 2.32 |
| 9 | 10.01 | 3.40 | 4.62 |
| 10 | 9.37 | 2.10 | 4.92 |
| 11 | 10.16 | 2.16 | 3.12 |
| 12 | 7.55 | 2.55 | 2.82 |
| 13 | 8.86 | 1.16 | 2.20 |
| 14 | 9.32 | 1.60 | 2.70 |
| 15 | 7.63 | 0.55 | 4.97 |
Part that the present invention does not relate to is the same as those in the prior art or can be realized by using the prior art.
Claims (3)
1. a kind of method in quick discrimination river crab difference husking period, it is characterized in that the following steps are included:
It is first turned on color difference meter, the correction of auto black and white plate is carried out first with blank, after correction, into canonical measure, will read
Modulus formula is adjusted to Lab mode, wherein and L value indicates the lightness of color ,+indicate partially white ,-indicate partially dark；A value indicates face
The green red value of color ,+indicate partially red ,-indicate partially green；B value indicates the yellow value of the indigo plant of color ,+indicate partially yellow ,-indicate partially blue；
River crab individual is then taken out, its carapace back is dried, the color for reading river crab carapace ectoskeleton with color difference meter is blue
Huang value (b)；
The taking-up river crab individual, its carapace back is dried and is carried out at low ambient temperatures, to reduce river crab vigor,
Convenient for measurement；
The low temperature environment, which refers to, is placed in environment temperature 3 minutes under 0-4 degrees celsius for the river crab individual of taking-up
Its carapace is dried again above；
Finally determine according to blue yellow value (b) work of the color of reading is following: as b >=7, individual is in proecdysis；When b≤
When 2, individual is in postmolt；As 2 < b < 7 of b value, individual is in intermolt.
2. the method according to claim 1, it is characterized in that the color difference meter is portable colorimeter.
3. the method according to claim 1, it is characterized in that the good place of daylighting indoors should be selected to carry out when measurement.
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| CN105145423B true CN105145423B (en) | 2019-03-01 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106305567A (en) * | 2016-08-21 | 2017-01-11 | 盘锦光合蟹业有限公司 | Image identification method used for judging river crabs |
| CN106719204B (en) * | 2017-01-06 | 2019-10-08 | 浙江省海洋水产研究所 | A method of soft crab is produced using wild crab |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7765953B2 (en) * | 2007-12-06 | 2010-08-03 | Chien-Hsun Ho | Caring shell for hermit crab |
| CN103004652A (en) * | 2012-10-12 | 2013-04-03 | 淮安正昌饲料有限公司 | Method for observing ecdysis period of crayfish |
| CN104488790A (en) * | 2014-12-24 | 2015-04-08 | 江苏省淡水水产研究所 | Device for observing molt and growth of river crabs and application thereof |
| CN204466602U (en) * | 2014-12-24 | 2015-07-15 | 江苏省淡水水产研究所 | A kind of observe river crab cast off a skin growth device |
-
2015
- 2015-07-31 CN CN201510460121.9A patent/CN105145423B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7765953B2 (en) * | 2007-12-06 | 2010-08-03 | Chien-Hsun Ho | Caring shell for hermit crab |
| CN103004652A (en) * | 2012-10-12 | 2013-04-03 | 淮安正昌饲料有限公司 | Method for observing ecdysis period of crayfish |
| CN104488790A (en) * | 2014-12-24 | 2015-04-08 | 江苏省淡水水产研究所 | Device for observing molt and growth of river crabs and application thereof |
| CN204466602U (en) * | 2014-12-24 | 2015-07-15 | 江苏省淡水水产研究所 | A kind of observe river crab cast off a skin growth device |
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Inventor after: Li Xuguang Inventor after: Zhou Gang Inventor after: Gu Xiaohong Inventor after: Zhou Jun Inventor after: Lin Hai Inventor after: Huang Hongbing Inventor after: Zeng Qingfei Inventor after: Mao Zhigang Inventor before: Li Xuguang Inventor before: Zhou Gang Inventor before: Gu Xiaohong Inventor before: Zhou Jun Inventor before: Lin Hai Inventor before: Huang Hongbin Inventor before: Zeng Qingfei Inventor before: Mao Zhigang |
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