CN104686456B - A kind of assay method of silkworm pupa developmental process - Google Patents
A kind of assay method of silkworm pupa developmental process Download PDFInfo
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- CN104686456B CN104686456B CN201510132574.9A CN201510132574A CN104686456B CN 104686456 B CN104686456 B CN 104686456B CN 201510132574 A CN201510132574 A CN 201510132574A CN 104686456 B CN104686456 B CN 104686456B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention discloses a kind of assay method of silkworm pupa developmental process, specifically includes following steps：（1）Build the mathematical model of the association of the silkworm pupa developmental process and metabolism decrement of kind to be detected；（2）After more than one Pupa bombycises identification male and female are taken in silkworm pupa to be determined, weigh the weight after pupating, further according to it is to be detected when weight, the accurate developmental process of silkworm pupa can be calculated according to male and female pupa mathematical model.The method can fill up the blank for not having Accurate Determining silkworm pupa developmental process method in the market, it is possible to reduce the artificial error for judging to bring with environmental change, easy to operate, be that the silkworm production of hybrid seeds and laboratory accurately judge that pupa development process provides new detection method.
Description
Technical field
The invention belongs to silkworm cultural technique field, and in particular to a kind of assay method of silkworm pupa developmental process, can be with
Accurately determine the process of pupa growth.
Background technology
Silkworm（Bombyx mori L.）In China by artificial domestication more than 5700 years, category Lepidoptera Bombycidae becomes completely
State insecticide, from generation to generation including ovum, larva, pupa and adult（Moth）Four worm states.Four worm state developments of silkworm are different, and inside and outside characterizes
Also it is different：Ovum is Embryonic Stages, and larva is the stage absorbed nourishment from the external world, and pupa is to complete larva to the abnormal mistake of moth
Cross the stage, adult is the stage for completing to raise up seed.It is as the pupa of silkworm need not absorb nourishment material from the external world, complete in vivo
, need to consume substantial amounts of energy into from larva organ to the transformation of adult organ, with the continuous consumption of energy, the weight of pupal cell
Constantly diminish, as female pupa needs to manufacture substantial amounts of silkworm seed, the development for causing male and female pupa of body has big physiological differences, directly
Cause there is difference in male and female pupal cell metabolism amount.
10～15 days silkworm pupa time, the more female pupa of development of male pupa are short 2～3 days, and people are in order to realize male and female pupa while incubating
Change, be easy to the production of hybrid seeds, needs to adjust the protection temperature in pupa time according to developmental process in time, this operating process is referred to as pupa time guarantor
Shield.
In order to determine the developmental process in pupa time, Pupa bombycises are protected in 25 ± 1 DEG C of temperature, in the environment of humidity 75～80%, root
According to the color change of pupal cell, and determine with reference to knowhow.As temperature and humidity indoor in actual production process can occur
Change, the coat color of some pupal cells is relatively deep in addition, uses conventional methods and is difficult to the development of Accurate Determining pupal cell and enters
Journey, this can bring impact to the arrangement of the production of hybrid seeds, in the urgent need to searching out the side that a kind of new detection pupa growth is carried out in production
Method.
The content of the invention
It is an object of the invention to provide a kind of assay method of silkworm pupa developmental process, conveniently, accurately to determine silkworm
The developmental process of pupa.
To achieve the above object of the invention, the technical solution used in the present invention is：A kind of measure side of silkworm pupa developmental process
Method, comprises the following steps：
（1）Build the relational model of the metabolism decrement and developmental process in silkworm pupa time to be detected；
（2）Silkworm pupa to be detected is taken as sample, the weight of 2～3 hours and sample after sample is pupated is determined and is developed one section
The weight during time, obtains metabolism decrement after sample development a period of time；Then model obtained by step (1) is compareed, according to sample
This metabolism decrement can determine the developmental process of silkworm pupa to be detected；
Step (1) specifically includes following steps:The pupa of silkworm to be detected is taken, is divided into two groups of Pupa bombycises of male and female；To pupate 2～3
In hour, the weight of pupal cell is starting weight, and the weight of a pupal cell was measured per 24 hours, until changing moth；Statistics pupal cell weight
Change and development time, set up the metabolism decrement and developmental process exponential equation of male and female pupa respectively, that is, obtain silkworm to be detected
The metabolism decrement and the relational model of developmental process in pupa time.
In above-mentioned technical proposal, step (1) protects Pupa bombycises under general environmental conditions to moth is changed, and can select temperature 25
DEG C, the condition of humidity 75%.
In above-mentioned technical proposal, due to the metabolic characteristicss difference in the pupa time of male and female pupa, the step（1）Male and female pupa should divide
Open founding mathematical models respectively.In concrete measure, silkworm pupa to be determined is taken, the weight of 2～3 hours after pupating is weighed, then is claimed
Weight when determining developmental process such as take, according to the sex of pupa, basis respectively（1）The male and female pupa mathematical model of foundation, Ke Yiji
Calculate the developmental process of pupal cell.
In above-mentioned technical proposal, the step（1）Some hypogenetic pupas, dead pupa, sick pupa and injury should be removed
Bad pupa, is further divided into two groups of male and female, measures, to reduce error.
In above-mentioned technical proposal, the step（1）Weigh the weight and step of pupal cell starting（2）Carrying out pupa growth
When process is determined, weigh as starting weight in 2～3 hours pupating, too early can be due to the excessively tender easy generation bleeding of pupal cell, mistake
Party affects the accuracy of mathematical model.
In above-mentioned technical proposal, the step（1）Female pupa group or male pupa group are chosen the Pupa bombycises of more than 10 and are weighed
Data, average, and the mathematical model of foundation is representative.
The method according to the invention, silkworm pupa starting weight are M1, and before changing moth, weight is M2, and metabolism decrement is M1-
M2；The development time in whole pupa time is 100%, during the model of the incidence relation for setting up silkworm pupa metabolism decrement and developmental process,
With pupal weight metabolism decrement as abscissa, with pupa development process as vertical coordinate, a standard curve is obtained；If development to be determined
The weight in stage is m, then developmental process can be expressed as（M1-M2）/（M1-m）×100%.
As above-mentioned technical proposal is used, the present invention has following advantages compared with prior art:
1., the invention provides a kind of assay method of silkworm pupa developmental process, its need is built respectively for a certain Silkworm varieties
Vertical male and female mathematical model, by weighing Pupa bombycises starting weight and the weight positioned at the stage of development to be measured, it is possible to accurately quick to survey
Determine the developmental process of pupa, solve the upper practical challenges of production, with result it is accurate, easy to operate, it is practical the characteristics of, be adapted to push away
It is wide to apply.
2. the first public assay method degree of accuracy height by metabolic determination silkworm pupa developmental process of the present invention, fills up mesh
There is no the blank of Accurate Determining silkworm pupa developmental process method on front market, it is possible to reduce what artificial judgement and environmental change brought
Error, it is easy to operate, judge that pupa development process provides new detection method for the industrial silkworm production of hybrid seeds and accurately.
Description of the drawings
Fig. 1 is the relativeness figure of male silkworm pupa metabolism decrement and pupa development process in embodiment one.
Fig. 2 is the relativeness figure of female Pupa bombycises metabolism decrement and pupa development process in embodiment two.
Specific embodiment
With reference to embodiment and accompanying drawing, the invention will be further described:
Embodiment one：A kind of assay method of silkworm hero pupa development process
（1）Material material and equipment：
The kind that silkworm pupa is bred as using University Of Suzhou（Su Xiu × spring is rich）, the kind is in 2010 by Jiangsu Province's silkworm product
Plant validation board's authorization, authorization numbering：Soviet Union examines silkworm 201001, possesses the universals that cultivated silkworm breed variety is raised in production, has
It is representative.Incubator is the 250D growth cabinets of Guangdong Province Shaoguan Xin Teng science popularization Instrument Ltd. production, and balance is Japanese
The electronic balance of a ten thousandth gram precision of Shimadzu Corporation's production.
Sample：Take 10 pupate after Pupa bombycises be used for model structure, separately take 1 pupate after Pupa bombycises be used for developmental process
Detection.
（2）It is placed on small straw bundles to spin cocoons and quantity collection
Silkworm is raised to being placed on small straw bundles to spin cocoons using conventional method, after being placed on small straw bundles to spin cocoons 3 days, lower Bombyx bombycis is adopted, Bombyx bombycis is cut out and is taken out the hair do not pupated
Foot silkworm, after unpupated larva pupates after 2 hours, weighs the weight of 10 male silkworm pupas respectively, records M1.Using 25 DEG C of temperature, humidity
75% condition protection Pupa bombycises are to change moth（Pupa skin ruptures）, a weight was weighed per 24 hours, it is M2 to change the weight before moth, respectively
The pupal cell weight of stage of development is shown in Table 1.
The weight of 1 different developmental phases silkworm of table hero pupa（g）
（3）Data processing and the structure of mathematical model.M1=1.24687g as shown in Table 1, M2=1.118g, weight loss
Total amount M1-M2=0.12887g；Pupa time metabolism slip (M1-M2)/M1*100% is defined as X, from pupating to moth propupa is changed
Phase metabolism slip is 10.34%；The percentage ratio that metabolism every day decrement accounts for total reduction is set to Y, Y is developmental process,
The mathematical model of Fig. 1, Y=9.6754X can be drawn（R2=1）.
（4）The measure of developmental process
According to the mathematical model set up, a male silkworm pupa is taken, weigh within two hours after pupating weight, M1=1.134g, often
Rule condition（25 DEG C of temperature, humidity 75%）When lower protection was by the 4th day, then weight is weighed for M2=1.102g, M1-M2=0.032
G, X=2.901%, according to the mathematical model of Fig. 1, Y=9.6754X, Y=28.068%, that is, have been detected the developmental process of Pupa bombycises
Jing reaches the 28.068% of whole pupa time.
（5）The checking of testing result
Determined male pupa is continued into protection to sprouting wings, pupa time elapsed-time standards is 11 days, if use conventional methods into
Row statistics, the developmental process of the 4th day should be 4/11 × 100%=36.363%, and the developmental process of the 3rd day should be
27.272%, cannot then subdivide between 27.272% to 36.363%, it is 28.068% that the present invention determines developmental process,
Data fall interval at the 4th day, it was demonstrated that the detection method of invention is accurate, and degree of accuracy is also higher than traditional method.
Embodiment two：A kind of assay method of the female pupa development process of silkworm
（1）Material material and equipment：
The kind that silkworm pupa is bred as using University Of Suzhou（Su Xiu × spring is rich）, the kind is in 2010 by Jiangsu Province's silkworm product
Plant validation board's authorization, authorization numbering：Soviet Union examines silkworm 201001, possesses the universals that cultivated silkworm breed variety is raised in production, has
It is representative.Incubator is the 250D growth cabinets of Guangdong Province Shaoguan Xin Teng science popularization Instrument Ltd. production, and balance is Japanese
The electronic balance of a ten thousandth gram precision of Shimadzu Corporation's production.
Sample：Take 10 pupate after female Pupa bombycises be used for model structure, separately take 1 pupate after female Pupa bombycises be used for develop
The detection of process.
（2）It is placed on small straw bundles to spin cocoons and quantity collection
Silkworm is raised to being placed on small straw bundles to spin cocoons using conventional method, after being placed on small straw bundles to spin cocoons 3 days, lower Bombyx bombycis is adopted, Bombyx bombycis is cut out and is taken out the hair do not pupated
Foot silkworm, after unpupated larva pupates after 2 hours, weighs the weight of 10 female Pupa bombycises respectively, records M1.Using 25 DEG C of temperature, humidity
75% condition protection Pupa bombycises are to change moth（Pupa skin ruptures）, a weight was weighed per 24 hours, it is M2 to change the weight before moth, respectively
The pupal cell weight of stage of development is shown in Table 2.
The weight of the female pupa of 2 different developmental phases silkworm of table（g）
（3）Data processing and the structure of mathematical model.M1=1.59723g as shown in Table 2, M2=1.43882g, body weight subtract
Few total amount M1-M2=0.15841g；Pupa time metabolism slip (M1-M2)/M1*100% is defined as X, from pupating to changing before moth
Pupa time metabolism slip is 9.92%；The percentage ratio that metabolism every day decrement accounts for total reduction is set to Y, Y is developmental process,
The mathematical model of Fig. 2, Y=10.083X can be drawn（R2=1）.
（4）The measure of developmental process
According to the mathematical model set up, a female Pupa bombycises are taken, weigh within two hours after pupating weight, M1=1.532g, often
When the 3rd day is protected under the conditions of rule, then weight is weighed for M2=1.508g, M1-M2=0.024 g, X=1.567%, according to Fig. 2
Mathematical model, Y=10.083X, Y=15.8%, that is, be detected Pupa bombycises developmental process reached the 15.8% of whole pupa time.
（5）The checking of testing result
Determined female pupa is continued into protection to sprouting wings, pupa time elapsed-time standards is 13 days, if use conventional methods into
Row statistics, the developmental process of the 3rd day should be 3/13 × 100%=23.077%, and the developmental process of the 2nd day should be
15.385%, cannot then subdivide between 15.385% to 23.077%, it is 15.8% that the present invention determines developmental process, number
It is interval at the 3rd day according to falling, it was demonstrated that the detection method of invention is accurate, and degree of accuracy is also higher than traditional method, becomes
Gesture is consistent with male pupa.
According to embodiment one and the result of embodiment two, the protection temperature in adjustment male silkworm pupa pupa time, carries out pupa time in time
Protection, realizes male and female pupa while hatching, is easy to the production of hybrid seeds.
Claims (4)
1. a kind of assay method of silkworm pupa developmental process, it is characterised in that comprise the following steps：
（1）Build the relational model of the metabolism decrement and developmental process in silkworm pupa time to be detected；
（2）Silkworm pupa to be detected is taken as sample, the weight of 2～3 hours and sample development a period of time after sample is pupated is determined
When weight, obtain sample development a period of time after metabolism decrement；Then model obtained by step (1) is compareed, according to sample
Metabolism decrement can determine the developmental process of silkworm pupa to be detected；
Step (1) specifically includes following steps：The pupa of silkworm to be detected is taken, is divided into two groups of Pupa bombycises of male and female；To pupate 2～3 hours
The weight of interior pupal cell is starting weight, and the weight of a pupal cell was measured per 24 hours, until changing moth；The change of statistics pupal cell weight
And development time, the metabolism decrement and developmental process exponential equation of male and female pupa are set up respectively, that is, obtain silkworm pupa time to be detected
Metabolism decrement and developmental process relational model；
The developmental process is the percentage ratio that metabolism every day decrement accounts for total reduction.
2. the assay method of silkworm pupa developmental process according to claim 1, it is characterised in that：Step (1) is in conventional environment
Under the conditions of protect Pupa bombycises to changing moth.
3. the assay method of silkworm pupa developmental process according to claim 1, it is characterised in that：The step（1）In, female silkworm
Pupa group or male pupa group choose the Pupa bombycises of more than 10.
4. the assay method of silkworm pupa developmental process according to claim 1, it is characterised in that：The step（1）In, surveying
Before amount pupal cell weight, the bad pupa of hypogenetic pupa, dead pupa, sick pupa and injury is removed.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002068916A (en) * | 2000-08-30 | 2002-03-08 | National Institute Of Agrobiological Sciences | Inducer to dormant egg of insect, and method for allowing dormant egg to be born |
| CN104026086A (en) * | 2014-06-17 | 2014-09-10 | 苏州大学 | Method for identifying pupation of domestic silkworms |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002068916A (en) * | 2000-08-30 | 2002-03-08 | National Institute Of Agrobiological Sciences | Inducer to dormant egg of insect, and method for allowing dormant egg to be born |
| CN104026086A (en) * | 2014-06-17 | 2014-09-10 | 苏州大学 | Method for identifying pupation of domestic silkworms |
Non-Patent Citations (1)
| Title |
|---|
| 《不同剂型溴虫腈在桑叶中残留对家蚕生长发育及产茧量的影响》;王冕等;《蚕业科学》;20091231(第3期);608-612 * |
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Effective date of registration: 20181130 Address after: 215228 North Ring Road, Shengze Town, Wujiang District, Suzhou City, Jiangsu Province, 1988 Patentee after: Jiangsu Hua Hao silk Limited by Share Ltd Address before: 215123 199 Ren Yan Road, Suzhou Industrial Park, Suzhou, Jiangsu Patentee before: Soochow University |
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