CN105145348B - A kind of seed selection of flue-cured tobacco sterile line new varieties and propagation method - Google Patents
A kind of seed selection of flue-cured tobacco sterile line new varieties and propagation method Download PDFInfo
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- CN105145348B CN105145348B CN201510729767.2A CN201510729767A CN105145348B CN 105145348 B CN105145348 B CN 105145348B CN 201510729767 A CN201510729767 A CN 201510729767A CN 105145348 B CN105145348 B CN 105145348B
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Abstract
The invention discloses a kind of seed selection of flue-cured tobacco sterile line new varieties and propagation method, methods described is originated using flue-cured tobacco cytoplasmic male sterile line MSG28 as Cytoplasmic male sterile genes, using MSG28 as female parent, it is recurrent parent with CZ 1, in the generation of continuous backcross 6 after hybridization, cultivate into novel fine cytoplasm male sterility line MSCZ 1；It is female parent with sterile line MSCZ 1, is male parent with maintainer CZ 1, outbreeding preserves sterile line MSCZ 1；The flue-cured tobacco sterile line MSCZ 1 of seed selection of the present invention has the advantages that sterility is stable, economical character is excellent, yield and output value is higher, easily the resistance to roasting property of roasting property is good, tobacco leaf outward appearance endoplasm is good, chemical composition is coordinated, and a new excellent sterile system germplasm is provided for flue-cured tobacco heterosis utilization research work.
Description
Technical field
The invention belongs to Tobacco Genetic Breeding technical field, and in particular to the seed selection of flue-cured tobacco cytoplasmic male sterile line and breeding
Method.
Background technology
Flue-cured tobacco has stronger Heterosis in terms of yield, disease resistance, breeding time, growth potential, but due to roasting
Tobacco interspecific hybridization is almost without obvious hybrid vigour in terms of quality of tobacco, and the work of flue-cured tobacco heterosis utilization is once baked
Cigarette breeder abandons.In recent years, because the sizing kind of seed selection in China's flue-cured tobacco breeding has breakthrough seldom, cause
National cigarette district main breed is single, and varietal resistance declines, the harm of pest and disease damage large area, China's cured tobacco production is caused serious
Loss.Flue-cured tobacco is due to stronger resistance hybrid vigour, and heterosis utilization cycle in breeding is short, instant effect spy
Point, domestic breeder starts to pay close attention to flue-cured tobacco heterosis utilization again again, assign it as a kind of breeding side quickly tackled
Method.
The performance of hybrid vigour is related to parent's genetic diversity, increases with being incremented by for parent's genetic distance.Therefore,
The flue-cured tobacco sterile line of seed selection genetic diversity, it is that flue-cured tobacco heterosis utilization is extremely important to expand the germ plasm resource that may be selected to utilize
Element task.
The content of the invention
The technical problems to be solved by the invention are, for need of the flue-cured tobacco heterosis utilization to sterile line genetic diversity
Ask there is provided a kind of seed selection of flue-cured tobacco sterile line new varieties and propagation method, excellent sterile system is provided for flue-cured tobacco heterosis utilization
Germplasm, widens the hereditary basis of flue-cured tobacco heterosis utilization, to overcome deficiency of the prior art.
Technical scheme：
A kind of selection of flue-cured tobacco sterile line new varieties：Using sterile line MSG28 as female parent, using CZ-1 as recurrent parent,
Sterile line new varieties MSCZ-1 is bred as by continuous backcross.
Foregoing selection, is comprised the following steps：
（1）Hybridization：Using sterile line MSG28 as female parent, using CZ-1 as male parent, hybridization obtains F1Seed；
（2）Continuous backcross：With F1For female parent, using CZ-1 as recurrent parent, backcrossing obtains BC1F1Seed；Using CZ-1 as samsara
Parent, and backcross progeny generation of continuous backcross 5 again, until obtaining BC6F1Seed, as sterile line new varieties MSCZ-1.
The propagation method of above-mentioned flue-cured tobacco sterile line new varieties：Using sterile line new varieties MSCZ-1 as female parent, using CZ-1 as
Male parent, breeds MSCZ-1.
The propagation method of foregoing flue-cured tobacco sterile line new varieties, is comprised the following steps：
（1）Plantation：Using MSCZ-1 as female parent, using CZ-1 as male parent, the sowing of the Parent same period, the same period transplant, male parent and mother
This is according to 1:Plant at 4 column number proportion intervals；
（2）Roguing:When cigarette strain is buddingged, hybrid strain is strictly gone, weak plant is gone, goes diseased plant, and thoroughly remove cigarette wooden fork, pressed
Type strain bagging is selected according to parent's standard；
（3）Artificial pollination：When MSCZ-1 corollas top red occurs without opening, exposed using manual method female
Stamen, is then evenly coated in CZ-1 pollen on the column cap of gynoecium；
（4）Timely sowing：According to the sequencing of hybridization pollination, ripe a collection of, harvesting is a collection of；Whenever capsule is into brown
When, you can harvest；The seed shady place of harvesting is air-dried, and threshing is after preservation in low temperature, drying, closed environment.
The sterile line MSCZ-1 that the present invention is bred as has the property that：
Floral organ is characterized as：Sterile line MSCZ-1 is consistent with maintainer CZ-1 inflorescence properties, is compact inflorescence, pink
Corolla, gynoecium normal growth, as staminody, staminody type sterile line.
Varietal character is characterized as：Economical character is excellent, and it is yellow good that tobacco leaf maturation falls, and the easily resistance to roasting property of roasting property is good；Comprehensive resistance compared with
By force, middle resistance to bacterial wilt, balck shank, rust, feel PVYNAnd CMV；Tobacco leaf presentation quality is good, color light orange, and colourity is strong, oil content
It is good；Tobacco leaf chemical composition is coordinated, and judging parameter is good.
1 feature
MSCZ-1 is transplanted to buddingging about 60 days, growing stage 122 days or so.Strain formula turriform, blade long avette, leaf color
Green, blade face is relatively put down, and blade tip is tapering, and leaf edge sawtooth shape, mesophyll tissue is loose, and cauline leaf angle is medium, and blade master pulse thickness is medium, armpit
Bud growing way is medium.Plant height of pinching 125cm, effective blade number 21 or so, pitch 6.15cm, stem girth 11.1cm, waist leaf is long
78.3m, waist leaf width 32.70cm, the long 66.20cm of top, waist leaf width 21.20cm.With parent K326 in plant height, leaf, leaf margin, leaf
There is variation in piece size, MSCZ-1 plant are higher, and pitch is slightly dilute, and waist leaf is longer wider, and other characters are suitable.
2 economic characters
MSCZ-1 per mu yield 151.39kg, 3409.81 yuan of average yield per mu value, 22.52 yuan/kg of average price, first-class cigarette ratio
Rate is 59.79%, and upper medium grade cigarette ratio is 72.46%.
3 qualities of tobacco
3.1 presentation quality
MSCZ-1 Maturity of Tobacco Leaf is based on maturation, and part is still ripe, and colourity is dense~strong, oil using " having " quality scale as
Main, small part oil is more, and blade construction is based on loose, and part is still loose, and identity is based on moderate, and small part identity is slightly
Tobacco leaf colourity and oil are better than control K326 in the middle part of thickness, MSCZ-1, and maturity, identity and blade construction are not as good as K326.
3.2 main chemical
The average nicotine content 2.89% of MSCZ-1 upper tobacco leafs, total nitrogen 1.87%, reduced sugar 21.95%, potassium 1.4%, chlorine
The average nicotine content 1.62% of tobacco leaf in the middle part of 0.28%, MSCZ-1, total nitrogen 1.58%, reduced sugar 27.5%, potassium 1.77%, chlorine 0.15%,
Compare with compareing K326, MSCZ-1 nicotine contents are suitable, and content of reducing sugar is slightly biased low, and potassium content is slightly biased low, chemical composition is coordinated
Property is suitable.
3.3 aesthetic quality
MSCZ-1 B2F rating sample Type of aroma belongs to flat-flavor, and preferably, fragrance richness is good for fragrance texture, fragrant
Makings, perfume quantity performance are slightly better than K326, and miscellaneous tolerance is lighter, and excitant is micro- to be had, and pleasant impression is relatively comfortable, and flue gas concentration is larger, cigarette
The fine and soft degree of gas is preferable, and strength is larger, good in taste, MSCZ-1 B2F rating samples totality judging parameter is slightly better than control K326；
MSCZ-1 C3F rating sample Type of aroma belongs to flat-flavor, and fragrance texture and style are better than K326, and fragrance richness is compared with K326
Good, flue gas concentration, strength are moderate, and miscellaneous gas is lighter, and excitant is micro- to be had, and pleasant impression is purer comfortable, and in good taste, flue gas is more fine and soft,
Flammability is stronger, and grey is good, MSCZ-1 C3F rating samples totality judging parameter is better than control K326.MSCZ-1 X2F grades
Sample Type of aroma belongs to flat-flavor, and aroma quality is slightly inferior and K326, and perfume quantity is suitable with K326, and miscellaneous gas, excitant are micro- to be had,
Flue gas concentration, strength are moderate, and pleasant impression is still comfortable, good in taste, and flammability is good, MSCZ-1 strains X2F rating samples are totally smoked panel test
Quality is slightly better than control K326.
Brief description of the drawings
Fig. 1 is sterile line MSCZ-1 seed selection flows of the present invention.
Embodiment
Embodiments of the invention：
The flue-cured tobacco cultivars MSG28 and CZ-1 that the present invention is used are planted from country of Dohanykutato Intezet of Qingzhou Tobacco Inst., China Tobacco Corp.
Matter resources bank is commercially available.
The selection of flue-cured tobacco sterile line new varieties, is comprised the following steps：
（1）Hybridization：Using sterile line MSG28 as female parent, using CZ-1 as male parent, hybridization obtains male sterility cross combination F1Kind
Son, F1Seed mixes sowing, and seed numbering is preserved；
（2）Continuous backcross：In next year, male sterility cross combination F is planted respectively1And CZ-1, with male sterility cross combination F1
For female parent, using CZ-1 as recurrent parent, backcrossing obtains BC1F1Seed, BC1F1Seed mixes sowing；In the follow-up time, with reference to sea
Nan Jiadai, using CZ-1 as recurrent parent, and backcross progeny generation of continuous backcross 5 again, until obtaining BC6F1Seed, it is substantially complete to reach
Into the purpose that MSG28 nucleus is replaced with CZ-1 nucleus, inheritance stability, completely sterile cytoplasmic male sterile line are cultivated into
MSCZ-1。
Wherein, " Hainan adds generation " refers to carrying out breeding material using the light and temperature condition not available for Hainan in Winter continent
Breeding plus generation, to accelerate breeding process.
Breeding male sterile lines new varieties MSCZ-1 method, is comprised the following steps：
（1）Plantation：Using MSCZ-1 as female parent, using CZ-1 as male parent, the sowing of the Parent same period, the same period transplant, male parent and mother
This is according to 1:Plant at 4 column number proportion intervals；
（2）Roguing:When cigarette strain is buddingged, hybrid strain is strictly gone, weak plant is gone, goes diseased plant, and thoroughly remove cigarette wooden fork, pressed
Type strain bagging is selected according to parent's standard；
（3）Artificial pollination：When MSCZ-1 corollas top occur it is red without it is open when, cut off with scissors corolla top or
Corolla is gently torn with finger, exposes gynoecium, then CZ-1 pollen is evenly coated on the column cap of sterile line gynoecium；
（4）Timely sowing：It is ripe a collection of according to the sequencing of hybridization pollination, harvest in time it is a collection of, whenever capsule is into brown
During color, maturation can be harvested seed, and the seed shady place of harvesting is air-dried, and threshing is after guarantor in low temperature, drying, closed environment
Deposit.
Claims (3)
1. a kind of selection of flue-cured tobacco sterile line new varieties, it is characterised in that comprise the following steps：
（1）Hybridization：Using MSG28 as female parent, using CZ-1 as male parent, hybridization obtains F1Seed；
（2）Continuous backcross：With F1For female parent, using CZ-1 as recurrent parent, backcrossing obtains BC1F1Seed；It is close by samsara of CZ-1
This, and backcross progeny generation of continuous backcross 5 again, until obtaining BC6F1Seed, as sterile line new varieties MSCZ-1.
2. breed the method for the flue-cured tobacco sterile line new varieties described in claim 1, it is characterised in that：Using MSCZ-1 as female parent, with
CZ-1 is male parent, breeds MSCZ-1.
3. the method for the flue-cured tobacco sterile line new varieties described in breeding claim 1 according to claim 2, its feature exists
In comprising the following steps：
（1）Plantation：Using MSCZ-1 as female parent, using CZ-1 as male parent, the sowing of the Parent same period, the same period are transplanted, and male parent is pressed with female parent
According to 1:Plant at 4 column number proportion intervals；
（2）Roguing:When cigarette strain is buddingged, hybrid strain is strictly gone, weak plant is gone, goes diseased plant, and thoroughly remove cigarette wooden fork, according to parent
This standard selects type strain bagging；
（3）Artificial pollination：When MSCZ-1 corollas top red occurs without opening, gynoecium is exposed using manual method, so
CZ-1 pollen is evenly coated on the column cap of gynoecium afterwards；
（4）Timely sowing：According to the sequencing of hybridization pollination, ripe a collection of, harvesting is a collection of；When capsule is into brown, i.e.,
It can harvest；The seed shady place of harvesting is air-dried, and threshing is after preservation in low temperature, drying, closed environment.
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| CN108834883B (en) * | 2018-08-29 | 2020-04-28 | 四川省烟草公司达州市公司 | Method for cultivating cigar tobacco without topping by utilizing male sterile line |
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| CN104885930A (en) * | 2015-07-01 | 2015-09-09 | 湖北省烟草科学研究院 | Breeding method for multi-resistance flue-cured tobacco hybrid |
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| CN104885930A (en) * | 2015-07-01 | 2015-09-09 | 湖北省烟草科学研究院 | Breeding method for multi-resistance flue-cured tobacco hybrid |
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| 不同烤烟品种( 系) 对比试验;史金钟等;《安徽农业科学》;20101231;第38卷(第19期);摘要、第2.4节 * |
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