CN105145339B - A kind of method for creating based on the high-quality mildew-resistance breeding element of haynaldia villosa - Google Patents
A kind of method for creating based on the high-quality mildew-resistance breeding element of haynaldia villosa Download PDFInfo
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- CN105145339B CN105145339B CN201510562257.0A CN201510562257A CN105145339B CN 105145339 B CN105145339 B CN 105145339B CN 201510562257 A CN201510562257 A CN 201510562257A CN 105145339 B CN105145339 B CN 105145339B
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Abstract
The invention discloses a kind of method for creating based on the high-quality mildew-resistance breeding element of haynaldia villosa, by the way that insertion system Nj2 among the apical segments section translocation line Nj1 or small fragment that carry haynaldia villosa mildew-resistance pm21 genes and sense powdery mildew wheat breed town wheat No. 168 or town wheat No. 8 are repeatedly returned, in combination with root-tip cells Metaphase Chromosomes genomic in situ hybridization technology（GISH）The homozygous exogenous chromosome family is screened from above backcross progeny, obtains that economical character is more consistent, cytogenetics is stable, and keeps the Small piece transposition homozygous plants of excellent powder mildew resistance, in order to which breeding family expenses are in the improvement of wheat breed.
Description
Technical field
The invention belongs to wheat breeding technical field, more particularly to one kind to be based on the high-quality mildew-resistance breeding element of haynaldia villosa
Method for creating.
Background technology
Wheat is to be distributed that most wide, cultivated area maximum, total output second, the volume of trade be most, nutritive value highest in the world
One of cereal crops, each wheat main production country always attaches great importance to wheat breed improvement.It is but intensive with Wheat Production
And modernization, the hereditary basis of cultivated wheat is increasingly narrow, and also more serious by environment and biotic, germ plasm resource is poor
The main bottleneck to be made a breakthrough as common wheat breeding.Contain abundant gene money in wheat wild kindred plant
Source.At present, it is found that from wheat relative category and has much led to quality, disease-resistant, pest-resistant, degeneration-resistant related gene, majority
The method for crossing distant hybridization and chromosome engineering is imported into Wheat Background.These new germ plasms are further cultivation new varieties (product
System) and intermediate materials established solid foundation.
Wild nearly edge species of the haynaldia villosa as wheat, to a variety of diseases such as powdery mildew, rust, eye spot and Wheat spindle streak mosaic
Evil all has more resistance, while good characteristics such as also dense cluster is spent more, cold-resistant is drought-enduring, grain protein content is high.Wheat and
After haynaldia villosa distant hybridization, by cytogenetic procedure, in offspring seed selection amphidiploid, alien addition line, alien substitution and
Translocation line, help to utilize the desirable genes of haynaldia villosa preferably in genetic improvement of wheat.This is for lifting the middle and lower reach of Yangtze River
Wheat breeding innovation ability, cultivation is high-quality, anti-disease wheat new varieties are very necessary.
The content of the invention
For overcome the deficiencies in the prior art, it is an object of the invention to provide one kind by external source is high-quality, mildew-resistance base
Because transformation to the middle and lower reach of Yangtze River area of wheat is promoted mainly in kind town wheat 168, town wheat No. 8, it is set to be directed to this area of wheat working condition and influence
The factors such as the Major Diseases of Wheat Production, quality-improving, there is the wheat breeding element method for creating of regional characteristics.
To reach above-mentioned purpose, the present invention is achieved by the following technical solutions：
A kind of method for creating based on the high-quality mildew-resistance breeding element of haynaldia villosa, comprises the following steps：
(1) selection carries the wheat work female parent of haynaldia villosa powdery mildew resistance gene Pm 21, selects No. 8 works of town Mai168Huo towns wheat
Male parent, F is obtained with male parent progress sexual hybridization by maternal1；
(2) by F1It is selfed again 1 time for 6 times with maternal first continuous backcross, obtains BC5F2；
(3) BC is selected using root-tip cells Metaphase Chromosomes genomic in situ hybridization technology (GISH)5F2Middle tool
Have exogenous chromosome import and for homozygous translocation chromosome individual plant；
(4) individual plant selfing obtained using step 3) 1 time, obtains high-quality mildew-resistance new lines town 10218.
The carrying haynaldia villosa mildew-resistance gene that further female parent is bred as by Agricultural University Of Nanjing's cytogenetics
Insertion system Nj2 among Pm21 apical segments section translocation line Nj1 or small fragment.
Further Small piece transposition the system Nj1 or Nj2 are improved from environmental resistance, protein content increases, high
The wheat seed of mildew-resistance.
Further the town wheat 168 is from mass of 1000 kernel is high, grain plumpness is good, the seed of sense powdery mildew.
Beneficial effect：Compared with prior art, the advantage of the invention is that：
1) high mildew-resistance.Carrying tuft is identified by the methods of Disease Resistance Identification, genomic in situ hybridization (GISH)
The identity information of wheat mildew-resistance gene pm21 Small piece transpositions system, under greenhouse powdery mildew artificial inoculation conditions, shows height
Mildew-resistance；
2) processing quality of recurrent parent is improved.Attributional analysis find seed crude protein content, wholemeal wet gluten content,
Stabilization time, grain hardness and sedimentation value are all remarkably higher than two Small piece transposition systems, suitable with strong gluten wheat town wheat 168, high
In middle gluten wheat town wheat No. 8；
3) Other Main Agronomic Characters are more coordinated.Through the comparative analysis with two Small piece transposition Xi He towns wheat economical characters,
Plant height, every plant of number of productive ear, spike length, every fringe spikelet number, number of grain per ear and mass of 1000 kernel are substantially better than Small piece transposition system.
Brief description of the drawings
Fig. 1 is the breeding procedures figure of the present invention.
Embodiment
With reference to example, the present invention is described in further detail.
Parent in the present invention can be bought disclosed in being.
Embodiment 1
A kind of method for creating based on the high-quality mildew-resistance breeding element of haynaldia villosa, comprises the following steps：As shown in figure 1,
(1) according to the requirement of breeding objective, the carrying haynaldia villosa for selecting Agricultural University Of Nanjing's cytogenetics to be bred as is anti-white
Powder ospc gene Pm21 apical segments section translocation line Nj1 makees maternal, the red skin of the careful high-quality strong gluten of state of Zhenjiang Academy of Agricultural Sciences seed selection
Wheat town wheat No. 168 makees male parent, and both are carried out into sexual hybridization obtains F1；
(2) hybrid F1With Nj1 elder generations of Small piece transposition system continuous backcross 6 times, then it is selfed 1 time, to keep recurrent parent
Superior resistance, break gene linkage, expand genetic recombination, obtain BC5F2Colony；
(3) root-tip cells Metaphase Chromosomes genomic in situ hybridization technology is utilized to step (2) colony individual plant
(GISH), screening includes external source small fragment and is the individual plant of homozygous translocation chromosome.
(4) individual plant selfing 1 time for obtaining step (3), the Quality Wheat mildew-resistance that economical character is more coordinated is obtained
Breeding element town 10218.
Embodiment 2
It is substantially the same manner as Example 1, except that the carrying tuft that selection Agricultural University Of Nanjing cytogenetics is bred as
Insertion system Nj2 makees maternal among the small fragment of wheat powdery mildew resistance gene Pm 21, and the state of Zhenjiang Academy of Agricultural Sciences seed selection examines excellent
Matter middle gluten wheat town wheat No. 8 makees male parent.
Embodiment 3
It is substantially the same manner as Example 1, except that the carrying tuft that selection Agricultural University Of Nanjing cytogenetics is bred as
Insertion system Nj2 makees maternal among the small fragment of wheat powdery mildew resistance gene Pm 21, and the state of Zhenjiang Academy of Agricultural Sciences seed selection examines excellent
The red skin wheat town wheat of matter strengthening tendons No. 168 makees male parent.
Embodiment 4
It is substantially the same manner as Example 1, except that the carrying tuft that selection Agricultural University Of Nanjing cytogenetics is bred as
Wheat powdery mildew resistance gene Pm 21 apical segments section translocation line Nj1 makees female parent, and the state of Zhenjiang Academy of Agricultural Sciences seed selection examines high-quality middle muscle
Wheat town wheat No. 8 makees male parent.
The advantages of here is new breeding element town 10218：
1st, high mildew-resistance.Under greenhouse powdery mildew artificial inoculation conditions, high mildew-resistance is shown.
Authentication method：Judged according to severity and leaf position disease level, the severity criteria for classifying：According to powdery mildew on incidence of leaf
Subiculum covering blade area accounts for the percentage of the blade gross area, by 1%, 5%, 10%, 20%, 40%, 60%, 80%,
100% totally 8 grade classifications, its proximity values is taken for the state of an illness between grade, though severity of having fallen ill less than 1%, by 1%
Remember (table 1)；It is light according to being gone up under wheat powdery mildew again, occurring rhythm from bottom to top, plant is from bottom to top divided into 9
Equal section, disease level is determined by the severity degree of different height disease leaf (or fringe).Occasionally there is morbidity early stage in field, after being
Just there is symptom in wheat strain upper blade or fringe (awns) in phase, and the situation of lower blade non-evident sympton, then according to severity
Difference, with reference to leaf position disease level drop 2-4 level deciding grade and level (table 2).
The wheat powdery mildew leaf position disease level classification standard scale of table 1
The wheat powdery mildew field resistance evaluation criterion table of table 2
| Resistance level | Victimization state |
| Immune (I) | Leaf position disease level, severity are 0 |
| Height is anti-(HR) | Leaf position disease level 1-2, severity is below 10% |
| Moderate resistance (MR) | Leaf position disease level 3-4, severity 10%-10% |
| Middle sense (MS) | Leaf position disease level 5-6, severity 40%-60% |
| Height sense (HS) | Leaf position disease level 7-9, severity more than 60% |
2nd, good processing quality.Seed crude protein content, wholemeal wet gluten content, Dry gluten content, gluten index
It is suitable with strong gluten wheat town wheat 168 and sedimentation value is all remarkably higher than two Small piece transposition systems, higher than middle gluten wheat town wheat No. 8.
Quality determination：
3rd, Other Main Agronomic Characters are more coordinated.Through the comparative analysis with two Small piece transposition Xi He towns wheat economical characters,
Plant height, every plant of number of productive ear, spike length, every fringe spikelet number, number of grain per ear and mass of 1000 kernel are substantially better than Small piece transposition system.
Economical character determines：
The present invention is illustrated according to above-described embodiment, it will be appreciated that above-described embodiment does not limit this in any form
Invention, all technical schemes obtained using equivalent substitution or equivalent transformation mode, is within the scope of the present invention.
Claims (1)
1. a kind of method for creating based on the high-quality mildew-resistance breeding element of haynaldia villosa, it is characterised in that comprise the following steps：
（1）The wheat that selection carries haynaldia villosa powdery mildew resistance gene Pm 21 makees maternal, selection town Mai168Huo towns wheat No. 8
Make male parent, F1 is obtained with male parent progress sexual hybridization by maternal；
（2）F1 is selfed 1 time for 6 times again with maternal first continuous backcross, obtains BC5F2；
（3）Being selected using root-tip cells Metaphase Chromosomes genomic in situ hybridization technology in BC5F2 has external source dye
Colour solid imports and is the individual plant of homozygous translocation chromosome；
（4）Utilize step（3）Obtained individual plant selfing 1 time, obtain high-quality mildew-resistance new lines town 10218；
The maternal Small piece transposition system Nj 1 or Nj2 for carrying haynaldia villosa powdery mildew resistance gene Pm 21, the small pieces
Section translocation line Nj1 or Nj2 is improved from environmental resistance, protein content increases, the wheat seed of high mildew-resistance, institute
Town wheat 168 is stated from mass of 1000 kernel is high, grain plumpness is good, the seed of sense powdery mildew.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104278028A (en) * | 2014-09-16 | 2015-01-14 | 天津师范大学 | Sequence of dasypyrum villosum 6VS DNA permeating into powdery mildew resistant near-isogenic line of wheat and application |
| CN104365471A (en) * | 2014-11-06 | 2015-02-25 | 南京农业大学 | Breeding and identifying method of soft and powdery mildew resistant triticum aestivum-Dasypyrum villosum translocation line |
| CN104561335A (en) * | 2015-01-16 | 2015-04-29 | 南京农业大学 | Breeding and molecular marker method of small-segment translocation line T1BL.1BS-2RL with wheat powdery mildew resistance |
-
2015
- 2015-09-07 CN CN201510562257.0A patent/CN105145339B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104278028A (en) * | 2014-09-16 | 2015-01-14 | 天津师范大学 | Sequence of dasypyrum villosum 6VS DNA permeating into powdery mildew resistant near-isogenic line of wheat and application |
| CN104365471A (en) * | 2014-11-06 | 2015-02-25 | 南京农业大学 | Breeding and identifying method of soft and powdery mildew resistant triticum aestivum-Dasypyrum villosum translocation line |
| CN104561335A (en) * | 2015-01-16 | 2015-04-29 | 南京农业大学 | Breeding and molecular marker method of small-segment translocation line T1BL.1BS-2RL with wheat powdery mildew resistance |
Non-Patent Citations (2)
| Title |
|---|
| Development and identification of wheat-Haynaldia villosa T6DL.6VS chromosome translocation lines conferring resistance to powdery mildew;H.Li, et al.;《Plant Breeding》;20051231;第124卷;第203-205页，尤其是第第203页右栏最后一段。 * |
| 携带抗白粉病基因Pm21的簇毛麦小片段易位系的改良;荣曼;《南京农业大学硕士学位论文》;20140815;摘要、第19页第1.1节以及表2-1。 * |
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Effective date of registration: 20210118 Address after: 225300 No.25 Chunhui Road, Zhangguo Town, Xinghua City, Taizhou City, Jiangsu Province Patentee after: Xinghua Lingyi science and Technology Consulting Service Co.,Ltd. Address before: Room 1406, 14 / F, unit 1, building 5, No. 366, Ronghua South Road, high tech Zone, Chengdu, Sichuan 610000 Patentee before: Sichuan qiyibiao Information Technology Co.,Ltd. Effective date of registration: 20210118 Address after: Room 1406, 14 / F, unit 1, building 5, No. 366, Ronghua South Road, high tech Zone, Chengdu, Sichuan 610000 Patentee after: Sichuan qiyibiao Information Technology Co.,Ltd. Address before: 212400 Hong Jing Road, Jurong City, Zhenjiang, Jiangsu Province, No. 1 Patentee before: JIANGSU FOOTHILL ZHENJIANG AGRICULTURE Research Institute |
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