CN105145339A - Creation method for dasypyrum villosum-based high-quality powdery mildew-resistant breeding element - Google Patents
Creation method for dasypyrum villosum-based high-quality powdery mildew-resistant breeding element Download PDFInfo
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- CN105145339A CN105145339A CN201510562257.0A CN201510562257A CN105145339A CN 105145339 A CN105145339 A CN 105145339A CN 201510562257 A CN201510562257 A CN 201510562257A CN 105145339 A CN105145339 A CN 105145339A
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Abstract
The invention discloses a creation method for a dasypyrum villosum-based high-quality powdery mildew-resistant breeding element. By backcrossing a top small fragment translocation line Nj1 or a small fragment intercalary translocation line Nj2 carrying the powdery mildew-resistant pm21 gene of dasypyrum villosum with a powdery mildew-infected wheat variety, Zhenmai No. 168 or Zhenmai No. 8, for multiple times and simultaneously screening a homozygous alien chromosome family from backcross descendants in combination with the genomic in-situ hybridization (GISH) of metaphase chromosomes of root-tip cell mitosis, a small fragment translocation homozygous plant which is uniform in agronomic traits and cytogenetically stable and keeps excellent powdery mildew resistance is obtained in order for breeders to improve wheat varieties.
Description
Technical field
The invention belongs to wheat breeding technical field, particularly relate to a kind of method for creating based on haynaldia villosa high-quality mildew-resistance breeding element.
Background technology
Wheat is one of the widest, cultivated area is maximum, gross yield second, the volume of trade maximum, nutritive value the is the highest cereal crops that distribute in the world, and each wheat main product state always attaches great importance to wheat breed improvement.But along with the intensive of Wheat Production and modernization, the hereditary basis of cultivated wheat is increasingly narrow, also more serious by environment and biotic, the poor Main Bottleneck having become common wheat breeding and made a breakthrough of germ plasm resource.Abundant genetic resources is contained in wheat wild kindred plant.At present, found a lot with quality, disease-resistant, pest-resistant, degeneration-resistant relevant gene from wheat relative belongs to, majority is imported in Wheat Background by the method for distant hybridization and chromosome engineering.These new germ plasms are for cultivating new varieties (strain) further and intermediate materials has established solid foundation.
Haynaldia villosa, as the wild near edge species of wheat, all has more resistance to multiple diseases such as powdery mildew, rust, eye spot and Wheat spindle streak mosaic, also has dense cluster to spend more, cold-resistant drought-enduring, the good characteristic such as grain protein content is high simultaneously.After wheat and haynaldia villosa distant hybridization, by cytogenetic procedure, seed selection amphidiploid, alien addition line, alien substitution and translocation line in offspring, contribute to the desirable genes utilizing haynaldia villosa better in genetic improvement of wheat.This, for lifting middle and lower reach of Yangtze River wheat breeding innovation ability, cultivates high-quality, anti-disease wheat new varieties is very necessary.
Summary of the invention
In order to overcome the deficiencies in the prior art, one is the object of the present invention is to provide to promote mainly in kind town wheat 168, town wheat No. 8 by external source high-quality, mildew-resistance gene transformation to middle and lower reach of Yangtze River Mai Qu, make it for Ben Mai district working condition and the factor such as Major Diseases, quality-improving affecting Wheat Production, there is the wheat breeding element method for creating of regional characteristics.
For achieving the above object, the present invention is achieved by the following technical solutions:
Based on a method for creating for haynaldia villosa high-quality mildew-resistance breeding element, comprise the steps:
(1) select the wheat carrying haynaldia villosa powdery mildew resistance gene Pm 21 to do maternal, select Mai168Huo town, town wheat No. 8 to make male parent, female parent and male parent are carried out sexual hybridization and obtains F
1;
(2) by F
1with the first continuous backcross of female parent 6 times selfing 1 time again, obtain BC
5f
2;
(3) root-tip cells Metaphase Chromosomes genomic in situ hybridization technology (GISH) is utilized to select BC
5f
2in there is exogenous chromosome import and be the individual plant of the translocation chromosome that isozygotys;
(4) step 3 is utilized) individual plant selfing that obtains 1 time, obtain high-quality mildew-resistance new lines town 10218.
Insertion system Nj2 in the middle of the apical segments section translocation line Nj1 carrying haynaldia villosa powdery mildew resistance gene Pm 21 that is bred as Agricultural University Of Nanjing's cytogenetics of described female parent or small fragment further.
Described Small piece transposition system Nj1 or Nj2 selects environmental resistance raising further, protein content increases, the wheat seed of high mildew-resistance.
Further described town wheat 168 select that thousand kernel weight is high, grain plumpness good, the seed of sense powdery mildew.
Beneficial effect: compared with prior art, the invention has the advantages that:
1) high mildew-resistance.Identify by methods such as Disease Resistance Identification, genomic in situ hybridization (GISH) identity information carrying haynaldia villosa mildew-resistance gene pm21 Small piece transposition system, under booth powdery mildew artificial inoculation conditions, show high mildew-resistance;
2) processing quality of recurrent parent is improved.Attributional analysis finds that seed crude protein content, wholemeal wet gluten content, stabilization time, grain hardness and sedimentation value are all significantly higher than two Small piece transposition systems, suitable with strong gluten wheat town wheat 168, higher than middle gluten wheat town wheat No. 8;
3) Other Main Agronomic Characters is comparatively coordinated.Through the comparative analysis with two Small piece transposition Xi He town wheat economical characters, plant height, every strain number of productive ear, spike length, every fringe spikelet number, number of grain per ear and thousand kernel weight are obviously better than Small piece transposition system.
Accompanying drawing explanation
Fig. 1 is breeding procedures figure of the present invention.
Embodiment
Below in conjunction with example, the present invention is described in further detail.
Can buy disclosed in Parent in the present invention is.
Embodiment 1
Based on a method for creating for haynaldia villosa high-quality mildew-resistance breeding element, comprise the following steps: as shown in Figure 1,
(1) according to the requirement of breeding objective, the apical segments section translocation line Nj1 carrying haynaldia villosa powdery mildew resistance gene Pm 21 selecting Agricultural University Of Nanjing's cytogenetics to be bred as does maternal, the state of Zhenjiang Academy of Agricultural Sciences seed selection examines high-quality strong gluten red skin wheat town wheat No. 168 and makes male parent, both is carried out sexual hybridization and obtains F
1;
(2) hybrid F
1with the first continuous backcross of Small piece transposition system Nj1 6 times, then selfing 1 time, to keep the superior resistance of recurrent parent, breaks gene linkage, expands genetic recombination, obtain BC
5f
2colony;
(3) utilize root-tip cells Metaphase Chromosomes genomic in situ hybridization technology (GISH) to step (2) colony individual plant, screening comprises external source small fragment and is the individual plant of translocation chromosome of isozygotying.
(4) individual plant selfing step (3) obtained 1 time, obtains the Quality Wheat mildew-resistance breeding element town 10218 that economical character is comparatively coordinated.
Embodiment 2
Substantially the same manner as Example 1, difference is that the middle insertion system Nj2 of the small fragment carrying haynaldia villosa powdery mildew resistance gene Pm 21 selecting Agricultural University Of Nanjing's cytogenetics to be bred as does maternal, and the state of Zhenjiang Academy of Agricultural Sciences seed selection examines Medium gluten wheat town wheat No. 8 and makes male parent.
Embodiment 3
Substantially the same manner as Example 1, difference is that the middle insertion system Nj2 of the small fragment carrying haynaldia villosa powdery mildew resistance gene Pm 21 selecting Agricultural University Of Nanjing's cytogenetics to be bred as does maternal, and the state of Zhenjiang Academy of Agricultural Sciences seed selection examines high-quality strong gluten red skin wheat town wheat No. 168 and makes male parent.
Embodiment 4
Substantially the same manner as Example 1, difference is that the haynaldia villosa powdery mildew resistance gene Pm 21 apical segments section translocation line Nj1 that carries selecting Agricultural University Of Nanjing's cytogenetics to be bred as does maternal, and the state of Zhenjiang Academy of Agricultural Sciences seed selection examines Medium gluten wheat town wheat No. 8 and makes male parent.
Here is the advantage in new breeding element town 10218:
1, high mildew-resistance.Under booth powdery mildew artificial inoculation conditions, show high mildew-resistance.
Authentication method: judge according to the sick level of severity and leaf position, the severity criteria for classifying: cover the percentage that blade area accounts for the blade gross area according to powdery mildew subiculum on incidence of leaf, by 1%, 5%, 10%, 20%, 40%, 60%, 80%, 100% totally 8 grade classification, its proximity values is got for the state of an illness between grade, though fallen ill but severity lower than 1%, by 1% note (table 1); Light according to heavily going up under wheat powdery mildew, occurring rhythm from bottom to top, is from bottom to top divided into 9 equal sections, determines disease level by the severity degree of the sick leaf (or fringe) of differing heights by plant.Field is even morbidity in early stage, and just the later stage just occurs symptom in wheat strain upper blade or fringe (awns), and the situation of lower blade non-evident sympton, then according to the difference of severity, with reference to the sick level in leaf position, 2-4 level deciding grade and level (table 2) is fallen.
Table 1 wheat powdery mildew leaf position sick level grade scale table
Table 2 wheat powdery mildew field resistance evaluation criterion table
| Resistance level | Victimization state |
| Immunity (I) | The sick level in leaf position, severity are 0 |
| High resistance (HR) | The sick level 1-2 in leaf position, severity less than 10% |
| In anti-(MR) | Leaf position sick level 3-4, severity 10%-10% |
| Middle sense (MS) | Leaf position sick level 5-6, severity 40%-60% |
| High sense (HS) | The sick level 7-9 in leaf position, severity more than 60% |
2, good processing quality.Seed crude protein content, wholemeal wet gluten content, Dry gluten content, gluten index and sedimentation value are all significantly higher than two Small piece transposition systems, suitable with strong gluten wheat town wheat 168, higher than middle gluten wheat town wheat No. 8.
Quality determination:
3, Other Main Agronomic Characters is comparatively coordinated.Through the comparative analysis with two Small piece transposition Xi He town wheat economical characters, plant height, every strain number of productive ear, spike length, every fringe spikelet number, number of grain per ear and thousand kernel weight are obviously better than Small piece transposition system.
Economical character measures:
The present invention is illustrated according to above-described embodiment, should be appreciated that above-described embodiment does not limit the present invention in any form, and all employings are equal to replacement or the technical scheme that obtains of equivalent transformation mode, all drop within protection scope of the present invention.
Claims (4)
1., based on a method for creating for haynaldia villosa high-quality mildew-resistance breeding element, it is characterized in that, comprise the steps:
(1) select the wheat carrying haynaldia villosa powdery mildew resistance gene Pm 21 to do maternal, select Mai168Huo town, town wheat No. 8 to make male parent, female parent and male parent are carried out sexual hybridization and obtains F
1;
(2) by F
1with the first continuous backcross of female parent 6 times selfing 1 time again, obtain BC
5f
2;
(3) root-tip cells Metaphase Chromosomes genomic in situ hybridization technology is utilized to select BC
5f
2in there is exogenous chromosome import and be the individual plant of the translocation chromosome that isozygotys;
(4) individual plant selfing utilizing step (3) to obtain 1 time, obtains high-quality mildew-resistance new lines town 10218.
2. a kind of method for creating based on haynaldia villosa high-quality mildew-resistance breeding element according to claim 1, is characterized in that, described female parent is for carrying Small piece transposition system Nj1 or Nj2 of haynaldia villosa powdery mildew resistance gene Pm 21.
3. a kind of method for creating based on haynaldia villosa high-quality mildew-resistance breeding element according to claim 2, is characterized in that, described Small piece transposition system Nj1 or Nj2 selects environmental resistance raising, protein content increases, the wheat seed of high mildew-resistance.
4. a kind of method for creating based on haynaldia villosa high-quality mildew-resistance breeding element according to claim 1, is characterized in that, described town wheat 168 selects that thousand kernel weight is high, grain plumpness good, the seed of sense powdery mildew.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104278028A (en) * | 2014-09-16 | 2015-01-14 | 天津师范大学 | Sequence of dasypyrum villosum 6VS DNA permeating into powdery mildew resistant near-isogenic line of wheat and application |
| CN104365471A (en) * | 2014-11-06 | 2015-02-25 | 南京农业大学 | Breeding and identifying method of soft and powdery mildew resistant triticum aestivum-Dasypyrum villosum translocation line |
| CN104561335A (en) * | 2015-01-16 | 2015-04-29 | 南京农业大学 | Breeding and molecular marker method of small-segment translocation line T1BL.1BS-2RL with wheat powdery mildew resistance |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104278028A (en) * | 2014-09-16 | 2015-01-14 | 天津师范大学 | Sequence of dasypyrum villosum 6VS DNA permeating into powdery mildew resistant near-isogenic line of wheat and application |
| CN104365471A (en) * | 2014-11-06 | 2015-02-25 | 南京农业大学 | Breeding and identifying method of soft and powdery mildew resistant triticum aestivum-Dasypyrum villosum translocation line |
| CN104561335A (en) * | 2015-01-16 | 2015-04-29 | 南京农业大学 | Breeding and molecular marker method of small-segment translocation line T1BL.1BS-2RL with wheat powdery mildew resistance |
Non-Patent Citations (2)
| Title |
|---|
| H.LI, ET AL.: "Development and identification of wheat-Haynaldia villosa T6DL.6VS chromosome translocation lines conferring resistance to powdery mildew", 《PLANT BREEDING》 * |
| 荣曼: "携带抗白粉病基因Pm21的簇毛麦小片段易位系的改良", 《南京农业大学硕士学位论文》 * |
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