CN105136748B - The identification screening installation and method for separating of a kind of vomitoxin - Google Patents
The identification screening installation and method for separating of a kind of vomitoxin Download PDFInfo
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- CN105136748B CN105136748B CN201510650692.9A CN201510650692A CN105136748B CN 105136748 B CN105136748 B CN 105136748B CN 201510650692 A CN201510650692 A CN 201510650692A CN 105136748 B CN105136748 B CN 105136748B
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Abstract
The identification screening installation of a kind of vomitoxin, including the first identification device, the second identification device；First identification device includes several feux rouges, and either infrared light light source and the first photoelectric detection unit feux rouges or infrared light light source are irradiated on material, and the first photoelectric detection unit receives the reflected light signal of material and is converted into electric signal；Second identification device includes several ultraviolet sources and the second photoelectric detection unit, and on ultraviolet source irradiation to material, the second photoelectric detection unit receives the fluorescence signal that material generates and is simultaneously converted into electric signal；It is identified above by the first identification device or the second identification device, it is that the material of stock is removed that any one, which is identified,.The present invention also provides a kind of method for separating.The advantage of the invention is that：It is combined by two kinds of identification technologies and sub-elects the exceeded particle of vomitoxin, can effectively reduced the vomitoxin in wheat, can be widely applied to grain processing field, have important practical significance and be widely applied prospect.
Description
Technical field
The present invention relates to food processing technology field, especially detection go mouldy the vomitoxin in wheat device and side
Method.
Background technology
Vomitoxin (DON) is widely present in the whole world, mainly pollutes the cereal crops such as wheat, barley, corn, has relatively strong
Cytotoxicity, humans and animals are after DON is eaten by mistake, it may appear that the symptoms such as apocleisis, vomiting, diarrhea, fever can cause dead when serious
It dies, research shows that, DON also has an impact immune system, there is apparent embryotoxicity and certain teratogenesis.China advises at present
It is 1mg/kg to determine the limit standard in cereal.
DON chemical property is highly stable, will not generally be destroyed in processing, storage, cooking process, there is stronger heat resist
Ability heats only a small amount of destruction in 25 minutes under the conditions of 121 DEG C, and acidic environment does not influence it, and alkaline environment can part
It destroys.
Current detection method main on the market is that thin layer chromatography, enzyme-linked immunization, gas phase liquid chromatography, fluorescence are exempted from
Epidemic disease analytic approach etc..These method quantitative precisions are high, but time-consuming, it is also higher that operating personnel are required for chemical detection pre-treatment,
It is difficult to meet online detection requirements.
Method currently used for reducing vomitoxin is broadly divided into two kinds, and one kind is cut down by chemical means, such as
Using in the method for ozone degradation wheat in CN201410653961 " a kind of method of vomitoxin in quick abatement wheat "
DON although this method abatement is efficient, reaches more than 80%, and ozone treatment takes very long, and treated that can wheat
It generates other harmful substance and whether has that unknown influence is in confused situation, the final material cut down by chemical method is state's domestic discipline and family rules
Surely market cannot be flowed to, without commercial value；Another method is to be surpassed vomitoxin by the means of photometric sorting
Target particle is detached from raw material, and obtained honest material can be used directly, and current existing technical literature records：2005
Delwiche et al. selects the vomitoxin in wheat samples using compound color selector outside assistant bamboo visible red, and the sorting wavelength of use is
675nm and 1480nm, bandwidth 30nm, content of toxins about reduces by 50% after sorting, but for the high wheat of some contents, after sorting
Vomitoxin content is still exceeded, and infrared visible composite construction is more complicated, is not easy to realize；Japan Saito in 2009 et al.
Two screening installation of the CS300 and RMG5561 sorting wheat vomitoxin for helping bamboo is compared, the results showed that using visible ray
The CS300 effects of sorting are preferable, but its separation characteristic is unknown, and is only identified with visible ray, toxin consistent to some appearance colors
The different material of content can not accurately identify, and market is also without related ripe product so far；2013 Nian Anyou groups grind
Study carefully institute king and change feature of the qin et al. to several typical wheat vomitoxin pollutions, it is indicated that normal wheat is red, vomitoxin
Exceeded particle is mainly not full grain, head blight grain and damaged particle, but does not provide specific method for separating and device,
In addition the feature of the exceeded grain of whole vomitoxins is not provided.
Invention content
In order to solve the problems, such as that existing wheat vomitoxin separation characteristic is indefinite and separation results are unstable, this patent will
The technical issues of solution is to provide the specific multiple contamination characteristics of wheat vomitoxin, and propose feux rouges or infrared light and
The method that Ultraluminescence is combined improves the discrimination of vomitoxin contamination characteristics, gives specific sorting unit.
Present inventor pass through on the basis of the feature of existing several typical wheat vomitoxins pollutions into
One step research obtains the feature of following wheat vomitoxin pollution：As shown in Figures 3 and 4, normal wheat is red wheat, feux rouges or
Person's infrared reflectivity wheat bigger than normal wheat has the particle that whitens, grizzled particle and damaged particle, the albumen for the particle that whitens
Matter content is low, and the damaged part of damaged particle is easily infected；Feux rouges or the infrared reflectivity wheat smaller than normal wheat
Mainly there is heterochromatic particle；In addition the smaller wheat vomitoxin content of particle is also higher, and feux rouges or infrared light can also be used
Illumination identification；In addition to this, the particle of appearance elephant skin also have vomitoxin, the method that ultraviolet induced fluorescence may be used come into
Row identification, is identified according to fluorescence color and fluorescence intensity.In the case of feux rouges or infrared light identify, it is normally set up two
A reflectivity threshold value of different sizes, it is exceeded all for vomitoxin more than big threshold value or less than small threshold value material
Material.Damaged particle much larger than any one material here, therefore does not provide spectrum since signal strength is very strong
Figure, grizzled particle are typically greyish white grain, and reflectivity will be less than white particle but higher than normal granules.
The technical issues of present invention uses the discrimination of the above-mentioned vomitoxin contamination characteristics of following technical scheme solution low：
A kind of identification screening installation of vomitoxin including blanking device, device for transporting objects, detection device, processor, rejects dress
It puts, material enters device for transporting objects by blanking device, and signal is passed to processor after detection material, handles
Device control device for eliminating rejects stock, and the detection device includes the first identification device, the second identification device；
First identification device includes several feux rouges either infrared light light source and the first photoelectric detection unit feux rouges or infrared
Radiant is irradiated on material, and the first photoelectric detection unit receives the reflected light signal of material and is converted into electric signal, passes through place
Device is managed according to criterion to being identified by the particle that vomitoxin pollutes；
Second identification device includes several ultraviolet sources and the second photoelectric detection unit, on ultraviolet source irradiation to material,
Second photoelectric detection unit receives the fluorescence signal that material generates and is converted into electric signal, by processor according to criterion pair
It is identified by the particle that vomitoxin pollutes；
Be identified above by the first identification device or the second identification device, any one identify be stock material
It is removed.
As one of embodiment, the device for transporting objects is slideway, several feux rouges or infrared light light
Source and the first photoelectric detection unit are located at the front of slideway, and several ultraviolet sources and the second photoelectric detection unit are located at slideway
Rear.
In the embodiment, first identification device can also include the first Background apparatus, and second identification device is also
It can include the second Background apparatus, first Background apparatus is located at the rear of slideway, and second Background apparatus is located at slideway
Front, without the region of material, feux rouges or infrared light light source are irradiated on the first Background apparatus, ultraviolet source irradiation to
On two Background apparatus, by material and the reflected light signal of the first Background apparatus reflection by the first photodetection of the first identification device
Unit receives, and the fluorescence signal that material and the second Background apparatus generate is connect by the second photoelectric detection unit of the second identification device
It receives.
As another embodiment, the device for transporting objects conveys crawler belt, several feux rouges or infrared for material
Radiant and the first photoelectric detection unit are located at material outside material conveying crawler belt and dish out above track, several ultraviolet sources
It is located at the surface of material conveying crawler belt with the second photoelectric detection unit.
In the embodiment, first identification device can also include the first Background apparatus, the first Background apparatus position
In the lower section of material conveying crawler belt, without the region of material, on ultraviolet source irradiation to belt, feux rouges or infrared light light source are shone
It is mapped on the first Background apparatus, by material and the reflected light signal of the first Background apparatus reflection by the first light of the first identification device
Electric probe unit receives.
The belt is black material.
In above-mentioned all schemes, optimization, the centre wavelength of the feux rouges or infrared light light source is more than 650nm, institute
The centre wavelength for stating ultraviolet source is less than 390nm.
Optimization, the center response wave band of first photoelectric detection unit is feux rouges or infrared band, described
The second photoelectric detection unit center response wave band be yellowish green optical band.
The present invention also provides a kind of method that identification screening installation using above-mentioned vomitoxin is sorted, packets
It includes：It is irradiated on material by feux rouges or infrared light light source, the reflected light that material is received using the first photoelectric detection unit is believed
Number and be converted into electric signal, by processor according to criterion to being identified by the particle that vomitoxin pollutes；
By the way that on ultraviolet source irradiation to material, the fluorescence signal of material generation is received simultaneously using the second photoelectric detection unit
Electric signal is converted into, by processor according to criterion to being identified by the particle that vomitoxin pollutes；
It is identified by both the above mode, it is that the material of stock is removed that any one, which is identified,.
Optimization, the centre wavelength of the feux rouges or infrared light supply is more than 650nm, the centre wavelength of the ultraviolet source
Less than 390nm, the center response wave band of first photoelectric detection unit is feux rouges or infrared band, described second
The center response wave band of photoelectric detection unit is yellowish green optical band.
The advantage of the invention is that：The technology identified by visible ray identification and ultraviolet induced fluorescence, which is combined to sub-elect, vomits
The exceeded particle of toxin is spat, the vomitoxin in wheat can be effectively reduced, can be widely applied to grain processing field, is had important
Realistic meaning and it is widely applied prospect.
Description of the drawings
Fig. 1 is the wheat vomitoxin sorting unit that first embodiment of the invention provides；
Fig. 2 is the wheat vomitoxin sorting unit that second embodiment of the invention provides；
Fig. 3 is the reflectivity spectral line of wheat vomitoxin visible features；
Fig. 4 is the fluorescent characteristics spectral line of wheat vomitoxin.
Critical piece title in part in figure：1. vibration feeding device；2. slideway；3. tested material；5. material conveys crawler belt；
6. belt；11. the first red-light source；12. the second red-light source；13. the first purple light light source；14. the second ultraviolet source；21. the
One photoelectric detection unit；22. the second photoelectric detection unit；31. the first Background apparatus；32. the second Background apparatus；41. baffle；
42. light-shading apparatus.
Specific embodiment
Purpose, technical scheme and advantage to make the embodiment of the present invention are clearer, below in conjunction with the embodiment of the present invention
In attached drawing, the technical solution in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
Part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
All other embodiments obtained without making creative work shall fall within the protection scope of the present invention.
First embodiment
Fig. 1 is the schematic diagram of the first embodiment of vomitoxin on-line measuring device of the present invention.The vomitoxin detect and
Sorting unit includes formering and later two the illumination detection unit based on different principle：First identification device, the second identification device, material
Slideway 2 and vibration feeding device 1.
First identification device includes the first red-light source 11, the second red-light source 12, the first photoelectric detection unit
21, the first Background apparatus 31.First red-light source 11, the second red-light source 12, the first photoelectric detection unit 21 are located at slideway 2
Front, the first Background apparatus 31 are located at the rear of slideway 2.
Second identification device includes the first ultraviolet source 13, the second ultraviolet source 14, the second photoelectric detection unit
22, the second Background apparatus 32.First ultraviolet source 13, the second ultraviolet source 14, the second photoelectric detection unit 22 are located at slideway 2
Rear, the second Background apparatus 32 are located at the front of slideway 2.
It is as follows to the process that rejecting is identified by the particle that vomitoxin pollutes by the first identification device：Material 3 passes through
It crosses vibration feeding device 1 and material shakes even, material 3 is transported to illumination detection zone by slideway 2, and the first of the first identification device is red
11 and second red-light source 12 of radiant is irradiated on material 3, without the region of material 3, then the first red-light source 11 and second
Red-light source 12 is irradiated on the first Background apparatus 31, by the reflected light signal that 3 and first Background apparatus 31 of material reflects by
First photoelectric detection unit 21 of one identification device is received and converted into electric signal, by processor according to criterion to quilt
Rejecting is identified in the particle of vomitoxin pollution.
Optimization, the first above-mentioned red-light source 11 and the second red-light source 12 are red-light LED or feux rouges line laser.
Further, the centre wavelength of the red-light LED or feux rouges line laser light source is more than 650nm, more preferably greatly
In 670nm.
Optimization, first Background apparatus 31 is bright background.
Further, the bright background is lan settings.
By the second identification device to by others to the process of rejecting is identified by the particle that vomitoxin pollutes such as
Under：Material 3 shakes material by vibration feeding device 1 even, and material 3 is transported to illumination detection zone by slideway 2, the second identification dress
The first ultraviolet source 13 and the second ultraviolet source 14 put are irradiated on material 3, and ultraviolet excitation goes out the glimmering of 3 surface of material
Light, without the region of material 3, the first ultraviolet source 13 and the second ultraviolet source 14 are irradiated on the second Background apparatus 32, material 3
The fluorescence signal generated with the second Background apparatus 32 is received by the second photoelectric detection unit 22 of the second identification device, is converted to electricity
Rejecting is identified to the particle of appearance elephant skin according to criterion by processor in signal.
It is either identified by the first identification device or is identified by the second identification device, as long as any dress
It puts and identifies it is stock, that is, rejected.
First ultraviolet source 13, the second ultraviolet source 14, the second photoelectric detection unit 22 are placed on a box 102
Interior, there are the inlet and outlet of light in face of the direction of material 3 for box 102, increase gear between the top of the box 102 and slideway 2
Plate 41 avoids influence of the extraneous visible ray to fluorescence signal.
Optimization, first ultraviolet source 13 and the second ultraviolet source 14 are ultraviolet LED or ultraviolet laser light source.
Further, the wavelength of the ultraviolet LED or ultraviolet laser light source should be less than 390nm, more optimize, wave
A length of 365nm.
Optimization, second Background apparatus 32 is black background or fluorescence dark background.Further, the black back of the body
Scape be stoving black varnish rectangular tube or round tube, the fluorescence dark background fluoresced by material other than color and sensor
The color being not responding to, it is such as red.
The sensor that above-mentioned the first photoelectric detection unit 21 and the second photoelectric detection unit 22 uses can be multi-color cord
Battle array CCD or monochromatic line array CCDs or CMOS.
The center response wave band of first photoelectric detection unit 21 is red spectral band, such as 600nm~1700nm.
The center response wave band of second photoelectric detection unit 22 is yellowish green optical band, usually in the second photodetection
The front end of unit 22 increases bandpass filter, and wavelength is 500~580nm.
Second embodiment
The present embodiment and first embodiment convey crawler belt 5, the second identification dress difference lies in device for transporting objects for material
It puts the surface for being located at material conveying crawler belt 5 respectively with the first identification device and material conveying 5 outside material of crawler belt is dished out track
Top.And second is not provided with Background apparatus in identification device.
Material 3 is first delivered to the second identification device, the first ultra violet illumination light source 13 and second by material conveying crawler belt 5
Ultra violet illumination light source 14 inspires the fluorescence on 3 surface of material, and the fluorescence signal that material 3 generates is connect by the second photoelectric detection unit 22
It receives, is converted to electric signal, do not have by processor according to criterion to rejecting is identified by the particle of other endotoxin contaminations
There is the region of material 3, the first ultraviolet source 13 and the second ultraviolet source 14 are irradiated on belt 6.
The belt 6 is black rubber or other black materials, is not fluoresced under ultraviolet irradiation.
The second identification device periphery would generally increase light-shading apparatus 42, avoid extraneous visible ray interference, be protected from light
There are certain spacing between device 42 and belt 6, ensure that material 3 can pass through.
With the rotation of motor, material 3 is moved to figure right, is dished out after reaching 6 edge of belt, reaches the first identification device
Detection zone, the first red-light source 11 and the second red-light source 12 of the first identification device are irradiated on material 3, without material
3 region, then the first red-light source 11 and the second red-light source 12 be irradiated on the first Background apparatus 31, by material 3 and first
The reflected light signal that Background apparatus 31 reflects is received and converted into electricity by the first photoelectric detection unit 21 of the first identification device
Signal, by processor according to criterion to rejecting is identified by the particle that vomitoxin pollutes.
The lighting source and detector is identical with the first embodiment.
3rd embodiment
The structure of the identification screening installation of the embodiment may be used disclosed in first embodiment or second embodiment
Structure, distinctive points are：Light source in first identification device is using infrared light light source, therefore, corresponding detection dress
Infrared detection device is set to, the detection method which uses is identical with first embodiment and second embodiment.
The foregoing is merely the preferred embodiments of the invention, are not intended to limit the invention creation, all at this
All any modification, equivalent and improvement made within the spirit and principle of innovation and creation etc., should be included in the invention
Protection domain within.
Claims (10)
1. a kind of identification screening installation of vomitoxin including blanking device, device for transporting objects, detection device, processor, picks
Except device, material enters device for transporting objects by blanking device, and signal is passed to processor after detection material,
Processor control device for eliminating rejects stock, it is characterised in that：The detection device includes the first identification device, the second identification
Device；
First identification device includes several red-light sources or infrared light light source and the first photoelectric detection unit, red-light source or infrared
Radiant is irradiated on material, and the first photoelectric detection unit receives the reflected light signal of material and is converted into electric signal, passes through place
Device is managed according to criterion to being identified by the particle that vomitoxin pollutes；
Second identification device includes several ultraviolet sources and the second photoelectric detection unit, on ultraviolet source irradiation to material, second
Photoelectric detection unit receives the fluorescence signal that material generates and is converted into electric signal, by processor according to criterion to being vomitted
The particle for spitting endotoxin contamination is identified；
Be identified above by the first identification device or the second identification device, any one identify be stock material i.e. quilt
It rejects.
2. the identification screening installation of vomitoxin according to claim 1, it is characterised in that：The device for transporting objects is
Slideway, several red-light sources or infrared light light source and the first photoelectric detection unit are located at the front of slideway, several purples
Outer light source and the second photoelectric detection unit are located at the rear of slideway.
3. the identification screening installation of vomitoxin according to claim 2, it is characterised in that：First identification device is also
Including the first Background apparatus, second identification device further includes the second Background apparatus, and first Background apparatus is located at slideway
Rear, second Background apparatus is located at the front of slideway, without the region of material, red-light source or infrared light light source irradiation
Onto the first Background apparatus, on ultraviolet source irradiation to the second Background apparatus, by material and the reflection of the first Background apparatus reflection
Optical signal is received by the first photoelectric detection unit of the first identification device, the fluorescence signal quilt that material and the second Background apparatus generate
Second photoelectric detection unit of the second identification device receives.
4. the identification screening installation of vomitoxin according to claim 1, it is characterised in that：The device for transporting objects is
Material conveys crawler belt, and several red-light sources or infrared light light source and the first photoelectric detection unit are located at outside material conveying crawler belt
Portion's material is dished out above track, several ultraviolet sources and the second photoelectric detection unit be located at material convey crawler belt just on
Side.
5. the identification screening installation of vomitoxin according to claim 4, it is characterised in that：First identification device is also
Including the first Background apparatus, first Background apparatus is located at the lower section of material conveying crawler belt, without the region of material, ultraviolet light
Source is irradiated on belt, and red-light source or infrared light light source are irradiated on the first Background apparatus, by material and the first Background apparatus
The reflected light signal of reflection is received by the first photoelectric detection unit of the first identification device.
6. the identification screening installation of vomitoxin according to claim 5, it is characterised in that：The belt is black material
Material.
7. the identification screening installation of vomitoxin according to any one of claims 1 to 6, it is characterised in that：The feux rouges
The centre wavelength of light source or infrared light light source is more than 650nm, and the centre wavelength of the ultraviolet source is less than 390nm.
8. the identification screening installation of vomitoxin according to claim 7, it is characterised in that：First photodetection
The center response wave band of unit is red spectral band or infrared band, and the center of second photoelectric detection unit responds wave
Section is yellowish green optical band.
9. the method sorted using the identification screening installation of claim 1 to 8 any one of them vomitoxin, feature
It is：Including two kinds of identification methods：
It is irradiated on material by red-light source or infrared light light source, the reflected light of material is received using the first photoelectric detection unit
Signal is simultaneously converted into electric signal, by processor according to criterion to being identified by the particle that vomitoxin pollutes；
By the way that on ultraviolet source irradiation to material, fluorescence signal and the conversion that material generates are received using the second photoelectric detection unit
Into electric signal, by processor according to criterion to being identified by the particle that vomitoxin pollutes；
It is identified by two ways, it is that the material of stock is removed that any one, which is identified,.
10. method for separating according to claim 9, it is characterised in that：The center of the red-light source or infrared light light source
Wavelength is more than 650nm, and the centre wavelength of the ultraviolet source is less than 390nm, and the center of first photoelectric detection unit is rung
Wave band is answered as feux rouges or infrared band, the center response wave band of second photoelectric detection unit is yellowish green optical band.
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| CN109848072A (en) * | 2019-01-08 | 2019-06-07 | 西安西北有色地质研究院有限公司 | Color dispersion-type Ultraluminescence color selector |
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| CN113857075B (en) * | 2021-10-11 | 2023-03-31 | 浙江大学 | Grain vomitoxin detection device and method based on polarized light and ultraviolet light imaging |
| CN115299552B (en) * | 2022-07-27 | 2024-04-05 | 中科芯禾(深圳)科技有限公司 | Grain toxin degradation method based on chip type high-power ultraviolet LED technology |
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