CN105132177A - Method for extracting tobacco leaf essential oil by using compound enzyme - Google Patents
Method for extracting tobacco leaf essential oil by using compound enzyme Download PDFInfo
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- CN105132177A CN105132177A CN201510339751.0A CN201510339751A CN105132177A CN 105132177 A CN105132177 A CN 105132177A CN 201510339751 A CN201510339751 A CN 201510339751A CN 105132177 A CN105132177 A CN 105132177A
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- tobacco leaf
- enzyme
- essential oil
- reaction
- leaf essential
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Abstract
The invention provides a method for extracting a tobacco leaf essential oil by using a compound enzyme. The method includes the following steps: homogenating tobacco leaves and a compound enzyme preparation solution, adding a buffer solution, adjusting the pH to 5.5-5.7, carrying out a reaction for 85-95 min at a reaction temperature of 52-58 DEG C, then distilling, and collecting a distillate, wherein the compound enzyme preparation solution contains a saccharifying enzyme, a compound plant hydrolase and hydrolysis protease. The method utilizes the compound enzyme preparation to extract aroma components in the tobacco leaves, and the aroma components are made into the tobacco leaf essential oil; and by optimization of the process conditions, and reasonable design of the reaction pH, temperature and time, the activity of the compound enzyme preparation is increased, so that the extraction rate of the tobacco leaf essential oil is greatly improved, and the production cost is reduced.
Description
Technical field
The present invention relates to a kind of method extracting tobacco leaf essential oil, more specifically, relate to a kind of method utilizing multiplex-enzyme extraction tobacco leaf essential oil.
Background technology
Research in recent years shows, tobacco leaf natural extract can increase cigarette flavor, improves the advantage such as Sensory Quality of Cigarette adding in cigarette and cigarette tissue, therefore, has more wide market and application prospect.But owing to producing upper extraction tobacco leaf natural extract at present, it is lower that product extracts yield, productive expense and cost higher, serious obstruction is produced and the developing of respective series product.Along with the progress of biotechnology in recent years, biological enzymolysis technology is utilized to extract tobacco leaf, thus realize increasing perfume quantity, alleviate blue foreign smell, eliminate the sense of peppery thorn, improve pleasant impression and improve advantages such as extracting yield, become the focus that current scientific worker pays close attention at present.
In recent years, for utilizing the research of cellulase treatment tobacco leaf also to achieve many achievements.Proteolytic enzyme is added the protein in the mixed system of tobacco and water in degrading tobacco by Gaisch etc., removes the polypeptide etc. in the aqueous solution, eliminate the protein of in tobacco more than 50% by microbial metabolism effect.Bernasek etc. utilize proteolytic enzyme to reduce protein tobacco content and make the tobacco sheet of lower protein content.The bentonite adsorption such as Bright removes soluble protein in the tabacco water solution after enzyme liberating.The tabacco water Proteins In Aqueous Solutions fragment that Grandpre etc. then utilize sodium laurylsulfonate to remove through enzyme liberating all achieves good effect.Yan Keyu etc. apply a certain amount of composite plant lytic enzyme and polygalacturonase in tobacco leaf, and result shows: cell wall substance can be made to be degraded to water-soluble sugar, degradable cell wall substance about 5% to 10%.Deng state guests etc., have carried out processing test to top cured tobacco leaf with polygalacturonase for material with Yuxi B3F tobacco leaf, at 50 DEG C of storage 12h.Detect through chemical composition and contrast smoking result and show, pectin substance reduces 18.15%, and the assorted gas in flue gas and pungency alleviate, and suction quality is improved.
But rare report is optimized enzymatic condition, can know according to enzyme kinetics, in Changing Regulation of Aroma Materials leaching process, terms and conditions is as processing parameters such as temperature of reaction, time, system pH, all affect speed and the intensity of enzymatic reaction, thus affect speed and the quantity of the generation of tabacco fragrance material, and finally affect the extraction yield of tobacco leaf.
In addition, China is tobacco leaf production big country, and in tobacco planting and the course of processing, create a large amount of discarded tobacco leafs, discarded tobacco leaf accounts for about 25% of tobacco ultimate production.Composition kind in discarded tobacco leaf and sound tobacco basically identical, but component content difference is larger, compared with sound tobacco, Mierocrystalline cellulose in discarded tobacco leaf, starch and protein content are relatively high, the compositions such as sugar alkali are relatively low, starch, the macromolecular substance such as protein make the assorted gas weightening finish of discarded tobacco leaf, fragrance reduces, greatly reduce the suction quality of leaf tobacco extract, therefore difficulty is brought to biological enzymolysis technology, therefore need to develop a kind of extraction yield that can improve discarded tobacco leaf, reduce production cost, improve the method for the suction quality of discarded tobacco leaf extract again.
Summary of the invention
The object of the present invention is to provide a kind of method utilizing multiplex-enzyme extraction tobacco leaf essential oil, described method effectively can improve the extraction yield of tobacco leaf.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
Utilize a method for multiplex-enzyme extraction tobacco leaf essential oil, comprise the following steps: tobacco leaf and compound enzymic preparation solution are carried out homogenate, adds buffered soln, pH is regulated to be 5.5 ~ 5.7, at temperature of reaction is 52 ~ 58 DEG C, reaction 85 ~ 95min, then distill, collect overhead product; Described compound enzymic preparation solution contains saccharifying enzyme, composite plant lytic enzyme and hydrolysising protease.
Biological enzyme is utilized to extract tobacco leaf essential oil, essence generation enzymatic reaction in the process extracted, can know according to enzyme kinetics, in Changing Regulation of Aroma Materials leaching process, terms and conditions is as temperature of reaction, time, the processing parameters such as system pH, all affect speed and the intensity of enzymatic reaction, thus affect speed and the quantity of the generation of tabacco fragrance material, and finally affect the extraction yield of tobacco leaf, and the optimum reaction condition of each enzyme is not identical, method of the present invention, by optimizing the pH of reaction, temperature and time, to coordinate saccharifying enzyme, optimum reaction condition between composite plant lytic enzyme and hydrolysising protease three kinds of enzymes, play the activity of compound enzymic preparation to greatest extent, thus greatly improve the extraction yield of tobacco leaf essential oil, extraction yield is greater than 6%.
Preferably, in described compound enzymic preparation solution, three kinds of enzymes form by following part by weight: saccharifying enzyme: composite plant lytic enzyme: hydrolysising protease is 20 ~ 30:350 ~ 450:150 ~ 200.
More preferably, in described compound enzymic preparation solution, three kinds of enzymes form by following part by weight: saccharifying enzyme: composite plant lytic enzyme: hydrolysising protease is 25:400:175.
Preferably, described tobacco leaf is discarded tobacco leaf.The method of the invention can not only effective enzymolysis discarded tobacco leaf, improves the extraction yield of discarded tobacco leaf, thus reduces production cost, ensure the suction quality of discarded tobacco leaf essential oil simultaneously.
Preferably, described pH is 5.6.
Preferably, described buffered soln is by 0.02mol/LNaH
2pO
4solution and 0.02mol/LK
2hPO
4solution composition.
Preferably, described temperature of reaction is 55 DEG C.
Preferably, the described reaction times is 91min.
Preferably, the time of described distillation is 70-80min.
Compared with prior art, the invention has the beneficial effects as follows:
The method utilizing multiplex-enzyme extraction tobacco leaf essential oil of the present invention, compound enzymic preparation is utilized to extract the aroma component in tobacco leaf, make tobacco leaf essential oil, and pass through pH, the temperature and time of Optimizing Technical, appropriate design reaction, improve the activity of compound enzymic preparation, thus greatly improve the extraction yield of tobacco leaf essential oil, reduce production cost.
Embodiment
Further describe the present invention by following specific embodiment, should be appreciated that specific embodiment described herein only for explaining the present invention, being not intended to limit the present invention, in the scope that can limit in right of the present invention, carry out various change.
The compound enzymic preparation adopted in the embodiment of the present invention 1 ~ 7 and comparative example 1 ~ 6 is by following configuration:
Get 0.01g saccharifying enzyme, add 1000g water, the massfraction being mixed with saccharifying enzyme is the enzyme solution A of 0.001%;
Get 0.16g composite plant lytic enzyme, add 1600g water, the massfraction being mixed with composite plant lytic enzyme is 0.01% enzyme solution B;
Get 0.07g hydrolysising protease, add 1400g water, the massfraction being mixed with hydrolysising protease is 0.005% enzyme solution C;
Get 100g enzyme solution A, 160g enzyme solution B, and 140g enzyme solution C, be mixed into 400g compound enzymic preparation.
Wherein saccharifying enzyme, composite plant lytic enzyme and hydrolysising protease are all from manufacturer-Novi's letter, saccharifying enzyme (AmylaseAG300L), density: 1.0 ~ 1.2g/ml, enzyme activity: 200 ~ 300AG/ml; Composite plant lytic enzyme (ViscozymeL), comprises arabanase, composite plant lytic enzyme, beta-glucanase, half composite plant lytic enzyme, zytase, density: 1.0 ~ 1.2g/ml, enzyme activity: 80 ~ 100FBG/g; Hydrolysising protease (Alcalase), enzyme activity: 1.2 ~ 2.4AU/g.
The present invention adopts volatile oil determination apparatud (the preset dimethylbenzene of the receptor in volatile oil determination apparatud) to carry out collection and measuration to overhead product.
embodiment 1
Preparation compound enzymic preparation solution, carries out homogenate by 200g discarded tobacco leaf and above-mentioned 400g compound enzymic preparation solution, adds by 0.02mol/LNaH
2pO
4solution and 0.02mol/LK
2hPO
4the buffered soln of solution composition, regulates pH to be 5.6, and at temperature of reaction is 55 DEG C, reaction 91min, is then slowly heated to boiling, and distillation 74min, collect overhead product, namely obtain tobacco leaf essential oil, the extraction yield of tobacco leaf essential oil is 7.05%.
embodiment 2 ~ 7
As different from Example 1, embodiment 2 ~ 7 adopts different pH, temperature of reaction, reaction times and distillation time, and other steps are identical with embodiment 1, and the extraction yield of its tobacco leaf essential oil is as shown in table 1:
Table 1
comparative example 1 ~ 6
As different from Example 1, comparative example 1 ~ 6 adopts different pH, temperature of reaction, reaction times and distillation time, and other steps are identical with embodiment 1, and the extraction yield of its tobacco leaf essential oil is as shown in table 2:
Table 2
Tobacco leaf essential oil is applied in the sensory evaluating smoking in cigarette tissue
By tobacco leaf essential oil preparation-obtained in the embodiment of the present invention 1 and embodiment 6, the ratio accounting for cigarette tissue quality 0.5% according to tobacco leaf essential oil to be added in cigarette tissue and to roll into cigarette, evaluation of smokeing panel test is carried out by professional, judging panel is totally 10 people, mark according to aroma quality, perfume quantity, flue gas concentration, assorted gas, pungency, pleasant impression, strength score and general comment mark respectively, then statistical average value, its result is as shown in table 3:
Table 3
Relatively can know from smoking result, the method for the invention substantially increases the extraction yield of tobacco leaf essential oil, and its effect improving sense organ is not deteriorated, and even slightly promotes.
Obviously, the above embodiment of the present invention is only for example of the present invention is clearly described, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.All any amendments done within the spirit and principles in the present invention, equivalent to replace and improvement etc., within the protection domain that all should be included in the claims in the present invention.
Claims (9)
1. kind to utilize the method for multiplex-enzyme extraction tobacco leaf essential oil, it is characterized in that, comprise the following steps: tobacco leaf and compound enzymic preparation solution are carried out homogenate, add buffered soln, regulate pH to be 5.5 ~ 5.7, at temperature of reaction is 52 ~ 58 DEG C, reaction 85 ~ 95min, then distills, and collects overhead product; Described compound enzymic preparation solution contains saccharifying enzyme, composite plant lytic enzyme and hydrolysising protease.
2. method according to claim 1, is characterized in that, in described compound enzymic preparation solution, three kinds of enzymes form by following part by weight: saccharifying enzyme: composite plant lytic enzyme: hydrolysising protease is 20 ~ 30:350 ~ 450:150 ~ 200.
3. method according to claim 2, is characterized in that, in described compound enzymic preparation solution, three kinds of enzymes form by following part by weight: saccharifying enzyme: composite plant lytic enzyme: hydrolysising protease is 25:400:175.
4. method according to claim 1, is characterized in that, described tobacco leaf is discarded tobacco leaf.
5. method according to claim 1, is characterized in that, described pH is 5.6.
6. method according to claim 1, is characterized in that, described buffered soln is by 0.02mol/LNaH
2pO
4solution and 0.02mol/LK
2hPO
4solution composition.
7. method according to claim 1, is characterized in that, described temperature of reaction is 55 DEG C.
8. method according to claim 1, is characterized in that, the described reaction times is 91min.
9. method according to claim 1, is characterized in that, the time of described distillation is 70 ~ 80min.
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| CN201510339751.0A CN105132177A (en) | 2015-06-18 | 2015-06-18 | Method for extracting tobacco leaf essential oil by using compound enzyme |
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| CN201510339751.0A CN105132177A (en) | 2015-06-18 | 2015-06-18 | Method for extracting tobacco leaf essential oil by using compound enzyme |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109355137A (en) * | 2018-11-21 | 2019-02-19 | 黛君本草生命科技（深圳）有限公司 | A kind of preparation process of cinnamon essential oil |
| CN109402088A (en) * | 2017-08-16 | 2019-03-01 | 郎玉珍 | Complex enzyme and preparation method thereof for extracting jasmine essential oil |
| CN112481036A (en) * | 2020-11-20 | 2021-03-12 | 河北中烟工业有限责任公司 | Enzymolysis preparation method of high-aroma tobacco essential oil |
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| CN1826997A (en) * | 2006-03-28 | 2006-09-06 | 华南理工大学 | Method for preparing tobacco essence by biological enzymolysis of inferior tobacco leaves |
| CN101204249A (en) * | 2007-12-11 | 2008-06-25 | 广东中烟工业公司 | Preparation method for tobacco extracting |
| CN101416777A (en) * | 2008-11-28 | 2009-04-29 | 华芳烟用香料有限公司 | Preparation method of tobacco extractum and use thereof |
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2015
- 2015-06-18 CN CN201510339751.0A patent/CN105132177A/en active Pending
Patent Citations (5)
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| CN1826997A (en) * | 2006-03-28 | 2006-09-06 | 华南理工大学 | Method for preparing tobacco essence by biological enzymolysis of inferior tobacco leaves |
| CN101204249A (en) * | 2007-12-11 | 2008-06-25 | 广东中烟工业公司 | Preparation method for tobacco extracting |
| CN101416777A (en) * | 2008-11-28 | 2009-04-29 | 华芳烟用香料有限公司 | Preparation method of tobacco extractum and use thereof |
| CN101940361A (en) * | 2010-10-11 | 2011-01-12 | 广东中烟工业有限责任公司 | Processing method of waste tobacco |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109402088A (en) * | 2017-08-16 | 2019-03-01 | 郎玉珍 | Complex enzyme and preparation method thereof for extracting jasmine essential oil |
| CN109355137A (en) * | 2018-11-21 | 2019-02-19 | 黛君本草生命科技（深圳）有限公司 | A kind of preparation process of cinnamon essential oil |
| CN112481036A (en) * | 2020-11-20 | 2021-03-12 | 河北中烟工业有限责任公司 | Enzymolysis preparation method of high-aroma tobacco essential oil |
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