CN105131347A - Preparation method of medium-concentration BA and glycerine gel - Google Patents

Abstract

The invention discloses a preparation method of medium-concentration BA and glycerine gel, and belongs to the field of plant biotechnology. The preparation method comprises the steps of: firstly preparing a BA mother liquid of 1 mg/mL; diluting the mother liquid to a medium-concentration BA and glycerine working liquid of 100 mg/L; and using the working liquid to prepare a medium-concentration blocking BA and glycerine gel liquid, wherein the mass concentration of carboxymethyl cellulose sodium is 1%, and the mass concentration of sodium alginate is 8%.

Description

The compound method of a kind of middle concentration BA and glycerogel

Technical field

A compound method for middle concentration BA and glycerogel, belongs to plant biotechnology field.

Background technology

Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's good character.Usually can use dissimilar hormone during cottage propagation, as BA, IBA, NAA, 2,4-D, GA ₃, 6-BA, KT, TDZ, TIBA, ethrel, B ₉, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agriculture production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutritive substance, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.

Summary of the invention

A compound method for middle concentration BA and glycerogel, is characterized in that, include hormone mother liquor, working fluid preparation, the preparation of hormone gel in described method, concrete operation step is as follows:

1. hormone mother liquor: precise weight is, after the KOH of a little 1M of BA of 0.1g dissolves, be settled to 100mL, be the BA mother liquor of 1mg/mL with distilled water;

2. working fluid preparation: it is concentration hormone working fluid in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, simultaneously according to the glycerine adding 10ml in the hormone working fluid of 90ml;

3. hormone gel preparation: accurately take 1g Xylo-Mucine and 8g sodium alginate is that the hormone of 100mg/L and glycerine working fluid dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition.

The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working fluid for solvent, Xylo-Mucine and sodium alginate are gel carrier, are mixed with containing gelating soln that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.

embodiment:

Below in conjunction with specific embodiment, the present invention is conducted further description.

Embodiment

A compound method for middle concentration BA and glycerogel, is characterized in that, include hormone mother liquor, working fluid preparation, the preparation of hormone gel in described method, concrete operation step is as follows:

1. hormone mother liquor: precise weight is, after the KOH of a little 1M of BA of 0.1g dissolves, be settled to 100mL, be the BA mother liquor of 1mg/mL with distilled water;

2. working fluid preparation: it is concentration hormone working fluid in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, simultaneously according to the glycerine adding 10ml in the hormone working fluid of 90ml;

3. hormone gel preparation: accurately take 1g Xylo-Mucine and 8g sodium alginate is that the hormone of 100mg/L and glycerine working fluid dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition.

Claims (1)

1. in, a compound method for concentration BA and glycerogel, is characterized in that, include hormone mother liquor, working fluid preparation, the preparation of hormone gel in described method, concrete operation step is as follows:

1. hormone mother liquor: precise weight is, after the KOH of a little 1M of BA of 0.1g dissolves, be settled to 100mL, be the BA mother liquor of 1mg/mL with distilled water;

2. working fluid preparation: it is concentration hormone working fluid in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, simultaneously according to the glycerine adding 10ml in the hormone working fluid of 90ml;

3. hormone gel preparation: accurately take 1g Xylo-Mucine and 8g sodium alginate is that the hormone of 100mg/L and glycerine working fluid dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition.