CN105126007B - A kind of patch for treating baby diarrhea - Google Patents
A kind of patch for treating baby diarrhea Download PDFInfo
- Publication number
- CN105126007B CN105126007B CN201510670816.XA CN201510670816A CN105126007B CN 105126007 B CN105126007 B CN 105126007B CN 201510670816 A CN201510670816 A CN 201510670816A CN 105126007 B CN105126007 B CN 105126007B
- Authority
- CN
- China
- Prior art keywords
- volatile oil
- oil
- patch
- ointment
- volatile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of patches for treating baby diarrhea, the patch includes the blank plaster with leakage-proof ring and the ointment being coated in the leakage-proof ring, the ointment is made of effective component, vitamin E and matrix, wherein, the effective component is made of the volatile oil of following weight percent amount: amomum fruit volatile oil 20.5~25.2%, Rhzoma Atractylodis Lanceae volatile oil 20.7~25.2%, Cortex Cinnamomi volatile oil 17.0~21.2%, volatile clove oil 29.1~35.4%；The volatile oil for forming the effective component is made by following methods: fructus amomi, rhizoma atractylodis, cortex cinnamomi and the medicinal powder of cloves taken respectively, and 15 times of amount water are added and impregnate 30 minutes, refluxing extraction under conditions of 100 DEG C；Volatile oil is collected, amomum fruit volatile oil, Rhzoma Atractylodis Lanceae volatile oil, Cortex Cinnamomi volatile oil and volatile clove oil are respectively obtained.The dosage of the vitamin E is the 1.5%~3.0% of effective component weight；The matrix is lanolin.Not only flavour of a drug are few for the patch, but also stabilization effect is good, significant in efficacy.
Description
Technical field
The present invention relates to the present invention relates to contain the pharmaceutical preparation for not determining structure from plant, and in particular to containing double
The emplastrum for not determining structure of cotyledon plant guiding principle rhizoma atractylodis platymiscium, the emplastrum can be used for treating diarrhea.
Background technique
Baby diarrhea be one group by more cause of diseases, it is multifactor caused by by times of defecation increase with stool change with the characteristics of
Pediatric common disease, 6 months -- 2 years old infant morbidity height causes infantile malnutrition, dysplasia and death
One of the main reasons.It being counted according to the World Health Organization (WHO), about 1,000,000,000 5 years old or less children suffer from diarrhea every year in the whole world,
In there are 4,000,000~5,000,000 children to die of diarrhea；One report of the United Nations in 2009 points out, the every 5 death of child cases in the whole world
In just there is a people to die of diarrhea.Domestic baby diarrhea is infectious strong, and disease incidence is high, and main pathogens are enteropathogenic E.Colis
And rotavirus, followed by shigella dysenteriae, detection of Salmonella, campylobacter jejuni and certain conditioned pathogens such as field coccus etc..The Ministry of Public Health will
It is classified as one of " four diseases " of paediatrics keypoint control, and prevention and control need to put into huge public health resources.
The mostly oral pipemidic acid of western medicine baby diarrhea, gentamicin, ampicillin, erythromycin or butylamine card at present
The antibiotic such as that mycin.Antibiotic generates strong influence to intestinal flora balance, they can inhibit or kill sensitive bacteria, promotes
Non-sensitive bacterium hyper-proliferative increases suprainfection chance；Quantity, position, the ratio of the normal strain of enteron aisle change, and lead to bacterium
Group's imbalance；Can also glucose absorption be made to reduce, disaccharide enzyme activity declines and diarrhea occurs；When largely killing bacterium, thallus is split
Solution produces the endotoxin of high concentration, and endotoxin, which enters blood, can increase permeability and peroxide injury, destroys gut barrier function,
The phagocytic function and general immunity for inhibiting liver reticuloendothelial system, to aggravate diarrhea or delay diarrhea.Pathogen
It makes a variation and newly the appearance of cause of disease, new antibiotic is expensive, the treatment mode based on antibiotic is also made to face huge challenge.
Traditional Chinese medicine prevention baby diarrhea has accumulated clinical experience abundant, wherein unique applying Chinese medicine to the umbilicus therapy occupies a seat
Ground.It, which has, applies convenient, cheap, treating both manifestation and root cause of disease advantage, is acted on by umbilicus absorption of drugs and medicine
Stimulation of the object to acupuncture point, it is shown that good therapeutic effect.Wherein representative drug be DINGHUIER QITIE but its
The effect for treating baby diarrhea is not still very ideal.In addition, eugenol contained by cortex cinnamomi and cloves and cinnaldehydrum are in illumination
It is extremely easy in decomposition under (4000lx), high temperature (60 DEG C~80 DEG C), has seriously affected the stability of the patch.Seminar's pre-stage test knot
Fruit shows that the accelerated degradation with effective component in patch, anti-diarrhea drug effect also decreased significantly, therefore containing unstable
Property effective component patch stabilisation measure, have important druggability value and significance.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of patch for treating baby diarrhea, not only flavour of a drug are few for the patch,
And stability is good, and it is significant in efficacy.
The scheme that the present invention solves above-mentioned technical problem is as follows:
A kind of patch for treating baby diarrhea, the patch include having the blank plaster of leakage-proof ring and being coated on described anti-
Ointment in leakage circle, the ointment are made of effective component, vitamin E and matrix, wherein
The effective component is made of the volatile oil of following weight percent amount: amomum fruit volatile oil 20.5~25.2%, grey
Art volatile oil 20.7~25.2%, Cortex Cinnamomi volatile oil 17.0~21.2%, volatile clove oil 29.1~35.4%；Have described in composition
The volatile oil of effect ingredient is made by following methods: taking fructus amomi, rhizoma atractylodis, cortex cinnamomi and the medicinal powder of cloves respectively, 15 times of amounts are added
Water impregnates 30 minutes, refluxing extraction under conditions of 100 DEG C；Volatile oil is collected, amomum fruit volatile oil, rhizoma atractylodis volatilization are respectively obtained
Oil, Cortex Cinnamomi volatile oil and volatile clove oil；
The dosage of the vitamin E is the 1.5%~3.0% of effective component weight；
The matrix is lanolin.
In above-mentioned ointment, the optimum proportioning of the effective component is amomum fruit volatile oil 23.43%, Rhzoma Atractylodis Lanceae volatile oil
23.85%, Cortex Cinnamomi volatile oil 20.08%, volatile clove oil 32.64%.
Patch of the present invention, wherein the blank plaster is commercially available blank plaster, the blank plaster
The characteristics of be middle part be equipped with leakage-proof ring.Above-mentioned blank plaster specifically can be Shandong Xiao Kang Biotechnology Co., Ltd, Anguo
Hong Sheng Medical Devices Co., Ltd. of city, Gaotang County Jia Ming Medical Devices Co., Ltd. or the limited public affairs of Beijing Tianyuan apricot woods medical sci-tech
The product of department.
Patch of the present invention can be used conventional method and be made, and the method that the present inventor recommends is as follows:
(1) it takes the effective component that vitamin E is added and stirs evenly；
(2) take the matrix, be placed in 100 DEG C of water-baths, water-bath to molten condition, taking-up is put to 50 DEG C, by effectively at
Fen ︰ matrix=1 ︰ 9 mass ratio is added the effective component containing vitamin E obtained by step (1) and is uniformly mixed, and medicine is made
Cream；
(3) ointment is coated on while hot in the leakage-proof ring of blank plaster by the dosage of every patch 0.2g, after cooling, is covered anti-
Glutinous layer to get.
Patch of the present invention presses the combination principle of Chinese medicine by the volatile oil group of fructus amomi, four taste of rhizoma atractylodis, cortex cinnamomi and cloves
At with dissipating dampness and activating spleen, the effect of warming middle energizer to stop diarrhea.Compared with existing DINGHUIER QITIE, the present invention considerably reduces mouse kind
It rushes down that leaf leads to diarrhea the wet bowel frequency number of model and writhing number, the total effective rate for treating baby diarrhea are up to 92%, is significantly higher than fourth osmanthus
The montmorillonite powder of youngster's navel patch and dioctahedral smectite.In addition, joined antioxidant vitamin E in effective component of the present invention, institute
Obtain finished product during storage, index components cinnaldehydrum, eugenol, Atisine chloride Atractydin retention rate significantly improve, and stabilization effect is good.
Specific embodiment
Technical effect possessed by the present invention will be proved by effect experiment, stability experiment, clinical trial below.
One, preparation stability and antidiarrheal drug effect influence to test
The discussion that 1 preparation stability influences antidiarrheal drug effect
1.1 experimental material
1.1.1 the preparation of Chinese medicine compound prescription volatile oil
Fructus amomi, rhizoma atractylodis, cortex cinnamomi and the medicinal powder of cloves are taken, 15 times of amount water is separately added into and impregnates 30 minutes, at 100 DEG C
Under the conditions of refluxing extraction；Volatile oil is collected, amomum fruit volatile oil, Rhzoma Atractylodis Lanceae volatile oil, Cortex Cinnamomi volatile oil and cloves volatilization are respectively obtained
Oil.Precision weighs amomum fruit volatile oil 5.6g, Rhzoma Atractylodis Lanceae volatile oil 5.7g, Cortex Cinnamomi volatile oil 4.8g, volatile clove oil 7.8g, sets
In 100ml brown measuring bottle, be ultrasonically treated 5 minutes, shake up, let cool to get.
1.1.2 the preparation of the volatilization ointment containing 13.0% prescription
Lanolin 8.70g (36-42 DEG C of fusing point) is weighed, 100 DEG C of water-baths, water-bath to molten condition are set, taking-up is put to suitable
1.1.1 lower prescription volatile oil 1.30g are added in suitable temperature, uniformly mixed, let cool to get.
1.1.3 the preparation of heated 0,10,15,20 day prescription volatilization ointment
It takes the above-mentioned 13.0% prescription volatilization ointment that contains each appropriate, is loaded in vial, seals, packed with fine aluminium bag,
To obtain the final product.It places 20 days under the conditions of 60 DEG C, was sampled in 0,10,15,20 day, measurement calculates These parameters ingredient retention rate.
1.1.4 check experiment group
Model control group: lanolin base
1.1.5 prepared by folium sennae leachate
Folium sennae medicinal powder 10g (20 mesh) is taken, is set in 200ml measuring bottle, the distilled water of 10 times of amounts is added, 90 DEG C are impregnated 0.5
Hour, filtration, take continuous 88 liquid to get.
1.1.6 animal:
NIH mouse is provided by Guangdong Medical Lab Animal Center, and SPF grades, Quality of Experimental Animals credit number:
SCXK (Guangdong) 2008-0020, male, 18~22g of weight.Feeding environment: 23 2 DEG C of room temperature, relative humidity 75
10%.
1.1.7 instrument reagent:
Folium sennae (is purchased from The First Affiliated Hospital of Guangzhou University of Traditional Chinese Med pharmacy)；Medicinal plant oil；Happy treasured pure water, happy treasured
Pure water Co., Ltd；Precise electronic assay balance, FA/JA series, upper current chart level instruments and meters Co., Ltd；Disposable injection
Device etc., 40, mouse cover；1, mouse box；Experimental bench.
1.2 experimental method
1.2.1 influence of the different pharmaceutical to folium sennae induced mice diarrhea
Male mice 60, it is randomly divided into 5 groups, fasting, while distilled water nursing is given, and after 4 hours, weighed weight, mould
Type control group presses 0.2g/ and only applies patch bare substrate, 0 day prescription volatilization ointment group of being heated, heated 10 days prescription volatilization ointments
Group, heated 15 days prescriptions volatilization ointment group, the heated 20 days volatilization ointment groups containing prescription are only applied that paste drug containing different by 0.2g/
Paste, after two hours, model control group, heated 0 day prescription volatilization ointment group, heated 10 days prescription volatilization ointment groups,
Heated 15 days prescription volatilization ointment groups fill folium sennae leaching by 0.3ml/10g weight containing prescription volatilization ointment group in heated 20 days
Liquid out, the observation of mouse single cage, lower berth blotting paper make muck counting, how much indicate diarrhoea degree with muck.Replacement blotting paper in time
Or cleaning muck, accumulative muck number, computation model control group, heated 0 day prescription volatilization ointment group, heated 10 are recorded per hour
Volatilize ointment group, heated 15 days prescriptions volatilization ointment group, heated 20 days volatilization each mouse 4 of ointment group containing prescription of its prescription is small
When interior muck sum.T is examined between carrying out group with each group muck number mean, as a result as follows.
1.2.2 statistical procedures
Experimental data is indicated with χ ± s, is examined using t, using SPSS17.0 software statistics packet carry out statistical analysis, * P <
0.05 indicates significant difference；* P < 0.01 indicates that difference is more significant；* * P < 0.001 indicates that difference is extremely significant.
1.2.3 experimental result
1 each group of table result (n=12) compared with model control group
* note: compared with model control group: P < 0.05 *；**P<0.01；***P<0.001；
Compared with heated 0 day prescription volatilization ointment group :+indicate P < 0.0；++ indicate P < 0.01；+++ indicate P < 0.001.
The synthesis retention rate of each index components in 2 60 DEG C of table heated 10,15,20 days oil-containing emplastrums
Note: comprehensive retention rate=cinnaldehydrum retention rate * 1.5/6+ eugenol retention rate * 2.5/6+ Atisine chloride Atractydin retention rate * 2/6
1.3 experiments discuss
The results show that 0 day prescription volatilization ointment group of being heated is to folium sennae induced mice diarrhea compared with model control group
There is apparent inhibiting effect；And 10,15,20 days prescription volatilization ointment groups of being heated respectively there are not folium sennae induced mice diarrhea
There is apparent inhibiting effect.The result shows that effective component palliating degradation degree increases, antidiarrheal drug effect with the extension in the period that accelerates the failure
In decreasing trend.
The confirmatory experiment that 2 preparation stabilities influence antidiarrheal drug effect
2.1 experimental material
2.1.1 the preparation of the volatilization ointment containing 13% prescription
Same 1.1.2.
2.1.2 the preparation of heated 0,10,15 day prescription volatilization ointment
Same 1.1.3.
2.1.3 check experiment group
Same 1.1.4.
2.1.4 prepared by folium sennae leachate
Same 1.1.5.
2.1.5 animal:
Same 1.1.6.
2.1.6 instrument reagent:
Same 1.1.7.
2.2 experimental method
2.2.1 influence of the different pharmaceutical to folium sennae induced mice diarrhea
Male mice 40, it is randomly divided into 4 groups, fasting, while distilled water nursing is given, and after 4 hours, weighed weight, mould
Type control group presses 0.2g/ and only applies patch bare substrate, 0 day prescription volatilization ointment group of being heated, heated 10 days prescription volatilization ointments
Group, heated 15 days prescription volatilization ointment groups only apply the different paste of patch drug containing by 0.2g/, after two hours, model control group,
Heated 0 day prescription volatilization ointment group, heated 10 days prescription volatilization ointment groups, heated 15 days prescription volatilization ointment groups are pressed
0.3ml/10g weight fills folium sennae leachate, the observation of mouse single cage, and lower berth blotting paper is made muck counting, how much indicated with muck
Diarrhoea degree.Timely replacement blotting paper or cleaning muck, record accumulative muck number per hour, and computation model control group is heated 0 day
Prescription volatilization ointment group, heated 10 days prescription volatilization ointment groups, heated 15 days prescriptions volatilize in each mouse of ointment group 4 hours
Muck sum.T is examined between carrying out group with each group muck number mean, as a result as follows.
2.2.2 statistical procedures
Ditto.
2.2.3 experimental result
3 each group of table and blank control group comparison result (n=10)
* note: compared with model control group: P < 0.05 *；**P<0.01；***P<0.001；
Compared with heated 0 day prescription volatilization ointment group :+indicate P < 0.0；++ indicate P < 0.01；+++ indicate P < 0.001.
2.4 experiments discuss
The results show that 0 day prescription volatilization ointment group of being heated is to folium sennae induced mice diarrhea compared with model control group
There is apparent inhibiting effect；And 10,15 days prescription volatilization ointment groups of being heated respectively do not have folium sennae induced mice diarrhea
Apparent inhibiting effect.The above verification test shows the repeatability of stability Yu drug effect association results, prompts this prescription or similar
The stabilisation measure of preparation has pharmacology meaning or clinical meaning.
Two, effect experiment
(1) mouse folium sennae leads to diarrhea model
1, experimental animal and grouping
80 NIH mouse, are provided by Guangdong Medical Lab Animal Center, and SPF grades, Quality of Experimental Animals credit number:
SCXK (Guangdong) 2008-0020, male, 18~22g of weight.Feeding environment: 23 2 DEG C of room temperature, relative humidity is 75 10%.80
Mouse is randomly divided into experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5, experimental group 6, control group and blank group, often
Group 10.
2, experimental method
Groups of animals fasting before being administered, while distilled water nursing is given, after 4 hours, weighed weight is simultaneously administered.Wherein, empty
White group is only smeared lanolin in the abdomen of NIH mouse by 0.2g/, and controlling application area is 3 ㎝2；Control group is sub- precious medicine company
The DINGHUIER QITIE of Group Plc's production；Experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5, experiment
Group 6 only successively smears the ointment of following embodiments 2,3,4,5,6,7 patch and control in the abdomen of NIH mouse by 0.2g/
Application area is 3 ㎝2.After being administered two hours, each group is given folium sennae leachate 0.3mL/10g by the weight of mouse and is filled
Stomach.The mouse single cage of stomach-filling is observed, and lower berth blotting paper makees muck counting, is added up muck number every a hour record and is replaced in time
How much blotting paper indicates diarrhoea degree with muck, is observed continuously 4 hours.
Above-mentioned folium sennae leachate the preparation method is as follows: take folium sennae medicinal powder 10g (20 mesh), set 200mL measuring bottle
In, add 10 times amount distilled water, 90 DEG C impregnate 0.5 hour, filtration, take subsequent filtrate to get.
The experimental data of record is indicated with ± s, is examined using t, carries out statistics using SPSS17.0 software statistics packet
Analysis, experimental result is shown in the following table 1.
4 each group of table (n=10) compared with model group antidiarrheal effect
* it infuses, compared with blank group, P < 0.05 indicates significant difference；* P < 0.01 indicates that difference is more significant；***P<0.001
Indicate that difference is extremely significant
3, conclusion
Seen from table 1, the effect of experimental group 1~6 is superior to control group.
(2) writhing analgesic experiment
1, experimental animal and grouping
Ibid.
2, experimental method
Groups of animals fasting before being administered, while distilled water nursing is given, after 10 hours, weighed weight is simultaneously administered.Wherein, empty
White group is only smeared lanolin in the abdomen of NIH mouse by 0.2g/, and controlling application area is 3 ㎝2；Control group is sub- precious medicine company
The DINGHUIER QITIE of Group Plc's production；Experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5, experiment
Group 6 only successively smears the ointment of following embodiments 2,3,4,5,6,7 navel patch and control in the abdomen of NIH mouse by 0.2g/
Application area is 3 ㎝2.After being administered two hours, 0.5% acetic acid 0.2mL/ is injected intraperitoneally only in each mouse, observes each group in 15min
There is the number of writhing response (abdomen indent, stretching, extension hind leg, buttocks are raised) in mouse and the time of writhing occurs in every mouse, counts
Calculate the analgesia percentage of each drug.
Analgesia percentage (%)=(blank group writhing response number-experimental group group writhing response number)/blank group writhing
React mouse number × 100%.
The experimental data of record is indicated with ± s, is examined using t, carries out statistics using SPSS17.0 software statistics packet
Analysis, experimental result is shown in the following table 5.
5 each group of table (n=10) compared with model group analgesic test
* it infuses, compared with blank group, P < 0.05 indicates significant difference；* P < 0.01 indicates that difference is more significant；***P<0.001
Indicate that difference is extremely significant
3, conclusion
By table 5 as it can be seen that the analgesia percentage of experimental group 1~6 can reach 60% or more；From the point of view of writhing number, experiment
Group 1~6 all has with blank group extremely significant difference (P < 0.05), while there were significant differences (P < 0.05) with control group；From averagely latent
From the point of view of lying prostrate the time, the average latency time of 1~6 writhing animal of experimental group extends nearly 2 times than blank group, and is all larger than control
Group.Therefore, the patch of the invention patent preparation has significant inhibitory effect to the pain as caused by acetic acid.
Three, skin irritation and anaphylaxis experiment
It standardizes, is inquired into containing tested according to " Chinese medicine, natural drug local irritation and hemolytic investigative technique guideline "
Irritation of 5%, 12.5%, the 20% Chinese medicine compound prescription volatile oil emplastrum of drug to rabbit intact skin；Investigate 12.5% Chinese medicine
Anaphylaxis of the compound volatile oil emplastrum to cavy.
1 experimental material
1.1 trial drug
1.1.1 test medicine 5% (g/g) volatilization ointment preparation
Lanolin 9.0g (36-42 DEG C of fusing point) is weighed, 100 DEG C of water-baths, water-bath to molten condition are set, taking-up is put to suitable
Temperature (50 DEG C), be added embodiment 1 volatile oil 0.47g, be uniformly mixed, let cool to get.(oil concentration 0.05g/g).
1.1.2 test medicine 12.5% (g/g) volatilization ointment preparation
Lanolin 9.0g (36-42 DEG C of fusing point) is weighed, 100 DEG C of water-baths, water-bath to molten condition are set, taking-up is put to suitable
Temperature (50 DEG C), be added embodiment 1 volatile oil 1.29g, be uniformly mixed, let cool to get.(oil concentration 0.125g/g).
1.1.3 test medicine 20% (g/g) volatilization ointment preparation
Lanolin 9.0g (36-42 DEG C of fusing point) is weighed, 100 DEG C of water-baths, water-bath to molten condition are set, taking-up is put to suitable
Temperature (50 DEG C), be added embodiment 1 volatile oil 2.25g, be uniformly mixed, let cool to get.(oil concentration 0.20g/g).
1.1.4 check experiment group
2,4- dinitro-chloro-benzene.
1.1.5 blank control group
Lanolin.
1.2 experimental animal
Cavy, weight (220 ± 30) g, half male and half female, Traditional Chinese Medicine University Of Guangzhou's animal experimental center provide SPF grades and move
Object；5~8 monthly age health Male New Zealand rabbit, 2.0~2.5Kg of weight are mentioned by Traditional Chinese Medicine University Of Guangzhou's animal experimental center
For.
1.3 test apparatus
Pet electric hair cutter, electric shaver, scalpel.
1.4 experimental situation
SPF grades of animal houses of Traditional Chinese Medicine University Of Guangzhou's zoopery environmental facility.
2 experimental methods
The irritant experiment of single and multiple dosing to rabbit intact skin: according to " Chinese medicine, natural drug local irritation
With hemolytic investigative technique guideline " specification development.
Cavy anaphylaxis experiment: select weight at 220 ± 3g cavy 9, be divided into 3 groups (n=3) at random, respectively by
Try medicine group, positive controls, blank control group.The symmetrical two sides in its back are lost hair or feathers in first 24 hours of test, the every side of area of losing hair or feathers
For 3cm × 3cm, and make following test:
(1) sensitization contact: hair removal section on the left of above-mentioned three groups of animals is pasted respectively and whitewashes lanolin 0.2g, containing 12.5% ointment
Agent 0.2g and positive sensitizer (1% 2,4- dinitro-chloro-benzene) 0.2mL.Time of contact continues 6 hours, and after administration 7,
14 days above-mentioned administration process of repetition.Wherein with one layer of disinfection oilpaper and two layers of antiseptic gauze covering, glue after positive controls administration
Cloth is fixed.
(2) excitation contact: in the sensitization contact stage, 14 days above-mentioned administration process of repetition, medicine-feeding part are the right side after the last administration
Side hair removal section, wherein 2, the 4- dinitro-chloro-benzene 0.2mL that positive sensitizer concentration is 1%.Drug is cleaned after excitation 6 hours,
At once it observes, then observed cutaneous anaphylaxis again in 24,48,72 hours, according to " Chinese medicine, natural drug immunotoxicity (mistake
Quick property, photoallergy) research technological guidance's principle " standards of grading evaluate.
Allergic reaction average value=(erythema forms total score+oedema and forms total score)/total number of animals
Sensitization incidence=animal number of cases/total number of animals of erythema, oedema or systemic anaphylaxis occur
* note: there is the animal number of cases of erythema, oedema or systemic anaphylaxis, no matter answering degree weight.
(3) cutaneous anaphylaxis degree standards of grading are as shown in the following table 6 and table 7:
The standards of grading of 6 cutaneous anaphylaxis degree of table
7 hypersensitive evaluation criterion of table
3 experimental results
3.1 single-dose rabbit intact skin irritation tests
8 intact skin single-dose of table tests dermoreaction integrated value
By table 8 as it can be seen that 5%, 12.5%, the 20% emplastrum single-dose containing volatile oil is 1,24,48 and 72 after removing drug
Hour is showed no the tested area of rabbit back intact skin and phenomena such as erythema, oedema occurs.Experimental result prompt, in single-dose man
In rabbit intact skin irritation test, test medicine is showed no skin irritation phenomenon.
3.2 multiple dosing rabbit intact skin irritation tests
9 intact skin multiple dosing of table tests dermoreaction average product score value and stimulus intensity
By table 9 as it can be seen that bare substrate and emplastrum containing volatile oil 5%, 12.5% are not in the test of intact skin multiple dosing
See irritation, 20% emplastrum containing volatile oil shows recoverable minimal irritation.
The skin hypersensitivity of 3.3 12.5% emplastrums containing volatile oil
It is standardized according to " technological guidance's principle that Chinese medicine, natural drug immunotoxicity (anaphylaxis, photoallergy) are studied ",
It inquires into containing 12.5% emplastrum of volatile oil to the skin hypersensitivity of cavy.Experimental result is as follows:
The skin hypersensitivity of 10 test medicine of table is evaluated
The skin hypersensitivity of 11 test medicine of table is evaluated
The results show that during the test, groups of animals does not occur the serious systemics such as asthma, astasia or shock
Allergic reaction.Wherein positive controls in 0h, for 24 hours, 48h and 72h sensitivity response mean scores be 1.3,3.7,5 and 6, sensitization rate
It is 100%.Blank control group and containing 12.5% volatilization ointment group sensitivity response average mark mean value be all 0, sensitization rate be 0%,
Prompt 12.5% emplastrum containing volatile oil has no sensitization to skin.
Conclusion
The volatile oil of embodiment 1 is chosen in this experiment, inquires into 5%, 12.5%, the 20% Chinese medicine compound prescription volatilization containing test medicine
Irritation and anaphylaxis of the oily emplastrum to rabbit intact skin.The result shows that bare substrate and containing volatile oil 5%, 12.5%
Emplastrum has no irritation, and 20% emplastrum containing volatile oil shows recoverable minimal irritation.It blank control group and is waved containing 12.5%
Hair oil paste group sensitivity response average mark mean value is all 0, and sensitization rate is 0%, to skin without sensitization.Animal irritation and allergy
Property experiment show that test medicine of the present invention has no irritation and allergic reaction.
Four, clinical effectiveness is investigated
1 general information
150 infants of this group are all from 2012~2013 years infants gone to a doctor to healthcare hospital for women & children.In strict accordance with " Chinese abdomen
Rush down disease diagnoses and treatment scheme " diagnostic criteria selection case, wherein male 85, female 65, the age 6~40 months, average 22
Month, 2~30d of the course of disease,
The clinical manifestation of above-mentioned case is that times of defecation is more, and daily 5~15 times, stool is in egg flower water sample or the dilute water of yellow
Sample, mostly with mild dehydration, fever, stool routine examination inspection has no pyocyte, phagocyte, and red blood cell, leucocyte are in every visual field
15 hereinafter, blood routine is showed no exception.Patient is randomly divided into control group 1, control group 2 and treatment group, every group 50.Control
Group 1 takes traditional treatment method, takes orally the montmorillonite powder of dioctahedral smectite；Control group 2 sticks infant Shenque using DINGHUIER QITIE
Cave；Treatment group selects the patch containing following embodiments 1 to stick infant SHENJUE acupoint.Two groups of infants the age, gender, weight, the state of an illness,
There was no significant difference for course of disease etc. (P > 0.05), is comparable.
2 treatment methods
Control group 1 only carries out conventional therapy, i.e., adjustment diet, 6~12h of fasting take orally the montmorillonite powder of dioctahedral smectite.Slightly
It is dehydrated patient's oral rehydration salts (ORS), middle severe dehydration person vein fluid infusion, correct dehydration and electrolyte disturbance.Control group 2 uses
DINGHUIER QITIE sticks infant SHENQUE acupoint, pastes for one time 1, dressing in 24 hours is primary.Treatment group is pasted using the navel of following embodiments 1,
One time a day, one time 1 patch, dressing in 24 hours are primary.Three groups as a treatment course with 3d, carries out therapeutic evaluation after a course for the treatment of.Patch
Apply during, if occur using uncomfortable situation as umbilical region is rubescent, ulceration, blistering if disable；It avoids pungent food during treatment, seafood, sheep
Meat, mushroom etc. send out object.
3 therapeutic evaluatioies
This experiment uses following standard, effective: by the treatment of a course for the treatment of, the daily times of defecation of infant is less than twice,
The character of stool and just routine inspection tend to normally, and clinical symptoms completely disappear；It is effective: by the treatment of a course for the treatment of, to suffer from
The daily times of defecation of youngster is less than four times, and the character of stool and just routine inspection make moderate progress, and clinical symptoms disappear substantially；Nothing
Effect: by the treatment of a course for the treatment of, infant clinical symptoms do not have any improvement exacerbation trend even occur.
4 statistical procedures
Database is established using statistics software SPSS 17.0, by t examine and2It examines and carries out statistical analysis, (P <
0.05) indicate that difference has significant.
5 results
Two groups of Clinical efficacy comparisons are shown in Table 12, and treatment group's total effective rate is 92% (46/50), and 1 total effective rate of control group is
76% (38/50), patient uses uncomfortable situation without appearance during 2 total effective rate of control group is 84% (42/50) and is administered,
Two groups of comparing differences are statistically significant (P < 0.05), and treatment group's curative effect is better than control group.The result shows that navel patch of the present invention
With significant clinical efficacy.
12 two groups of infant clinical therapeutic efficacies of table compare [example (%)]
* note: compared with the control group: * indicates P < 0.05；Total effective rate=(effective number of cases+effective number of cases)/total number of cases ×
L00%.
Five, antioxidant is influenced research by thermal stability to Chinese medicine compound volatile oil
1. antioxidant type screening study
1.1 experimental material
1.1.1 the preparation of Chinese medicine compound prescription volatile oil
Fructus amomi, rhizoma atractylodis, cortex cinnamomi and the medicinal powder of cloves are taken, 15 times of amount water is separately added into and impregnates 30 minutes, at 100 DEG C
Under the conditions of refluxing extraction；Volatile oil is collected, amomum fruit volatile oil, Rhzoma Atractylodis Lanceae volatile oil, Cortex Cinnamomi volatile oil and cloves volatilization are respectively obtained
Oil.Precision weighs amomum fruit volatile oil 5.6g, Rhzoma Atractylodis Lanceae volatile oil 5.7g, Cortex Cinnamomi volatile oil 4.8g, volatile clove oil 7.8g, sets
In 100ml brown measuring bottle, be ultrasonically treated 5 minutes, shake up, let cool to get.
1.1.2 the preparation of the emplastrum containing 0.2%VE
It takes 1.1.1 lower prescription volatile oil spare, separately weighs lanolin 45.0g (36-42 DEG C of fusing point), set 60 DEG C of water-baths
Pot, water-bath to molten condition, taking-up are put to preference temperature, and VE oil 0.2g (VE content 0.5mg/ is added in volatile oil about 5.0g
Mg), it is uniformly mixed, lets cool with matrix.Each 1.5g of paste of group containing VE is taken, is filled in blank patch, seals, is packed with fine aluminium bag,
To obtain the final product.(emplastrum is 0.1g/g containing concentration, and VE dosage is the 2% of volatile oil weight)
1.1.3 the preparation of the emplastrum containing 0.2%BHT
It takes 1.1.1 lower prescription volatile oil spare, separately weighs lanolin 45.0g (36-42 DEG C of fusing point), set 60 DEG C of water-baths
Pot, water-bath to molten condition, taking-up are put to preference temperature, and BHT0.1g is added in volatile oil about 5.0g, and (2,6- di-t-butyls are to first
Phenol), it is uniformly mixed, lets cool with matrix.Each 1.5g of paste of group containing VE is taken, is filled in blank patch, seals, is packed with fine aluminium bag,
To obtain the final product.(being 0.1g/g containing concentration, BHT dosage is the 2% of volatile oil weight)
1.1.4 the preparation of the emplastrum containing 0.2%BHA
It takes 1.1.1 lower prescription volatile oil spare, separately weighs lanolin 45g (36-42 DEG C of fusing point), set 60 DEG C of water-baths,
To molten condition, taking-up is put to preference temperature for water-bath, and BHA0.1g is added in volatile oil about 5.0g, and (tert-butyl is to hydroxyl fennel
Ether), it is uniformly mixed, lets cool with matrix.Each 1.5g of paste of group containing VE is taken, is filled in blank patch, seals, is packed with fine aluminium bag,
To obtain the final product.(being 0.1g/g containing concentration, BHA dosage is the 2% of volatile oil weight)
1.2 methods and result
1.2.1 test method
Above-mentioned sample is set in sealing clean container, places 5 days under the conditions of 40 DEG C, 60 DEG C, was taken in 0,3,5 day respectively
Sample, measurement calculate These parameters ingredient retention rate.
1.2.2 test solution preparation and measurement
Emplastrum about 0.25g is weighed, it is accurately weighed, it sets in stuffed conical flask, accurate addition methanol 25ml, weighed heavy respectively
Amount, ultrasonic 45min is let cool, then weighed weight, and the weight of less loss is supplied with methanol, is shaken up, and is filtered, and takes subsequent filtrate to get for examination
Product solution.It is appropriate to draw above-mentioned each test solution, miillpore filter filtration takes subsequent filtrate, as a result hplc determination is seen below
Table.
Table 13 does not add the high temperature test result of antioxidant emplastrum
The high temperature test result of 14 emplastrum containing 0.2%VE of table
The high temperature test result of 15 emplastrum containing 0.2%BHT of table
The high temperature test result of 16 emplastrum containing 0.2%BHA of table
1.3 experiments discuss
The results show that compared with the emplastrum that antioxidant is not added, cinnaldehydrum, fourth in 40 DEG C of heated 5 days emplastrums containing VE
Fragrant phenol, Atisine chloride Atractydin retention rate slightly above contain BHT emplastrum and emplastrum containing BHA；In heated 5 days emplastrums containing VE of higher temperature 60 C
Middle cinnaldehydrum, eugenol, Atisine chloride Atractydin reservation significantly improve, and are significantly higher than emplastrum containing BHT and emplastrum containing BHA, under this condition
VE is significantly better than BHT to the protecting effect of index components in emplastrum and containing BHA.Follow-up test further investigates VE pairs of different amounts
The protecting effect of index components in emplastrum.
2 antioxidant VE dosages are investigated
2.1 experimental material
2.1.1 the preparation of the emplastrum containing 0.01%VE
It takes 1.1.1 lower prescription volatile oil spare, separately weighs lanolin 45.0g (36-42 DEG C of fusing point), set 60 DEG C of water-baths
Pot, water-bath to molten condition, taking-up are put to preference temperature, and VE oil 0.01g (VE content 0.5mg/ is added in volatile oil about 5.0g
Mg), it is uniformly mixed, lets cool with matrix.Each 1.5g of paste of group containing VE is taken, is filled in blank patch, seals, is packed with fine aluminium bag,
To obtain the final product.(being 0.1g/g containing concentration, VE dosage is the 0.1% of volatile oil weight)
2.1.2 the preparation of the emplastrum containing 0.05%VE
With the preparation of the emplastrum containing 0.01%VE, VE oil 0.05g (VE content 0.5mg/mg) is added in volatile oil about 5.0g, i.e.,
?.(being 0.1g/g containing concentration, VE dosage is the 0.5% of volatile oil weight)
2.1.3 the preparation of the emplastrum containing 0.10%VE
With the preparation of the emplastrum containing 0.01%VE, VE oil 0.10g (VE content 0.5mg/mg) is added in volatile oil about 5.0g, i.e.,
?.(being 0.1g/g containing concentration, VE dosage is the 1% of volatile oil weight)
2.1.4 the preparation of the emplastrum containing 0.15%VE
With the preparation of the emplastrum containing 0.01%VE, VE oil 0.15g (VE content 0.5mg/mg) is added in volatile oil about 5.0g, i.e.,
?.(being 0.1g/g containing concentration, VE dosage is the 1.5% of volatile oil weight)
2.1.5 the preparation of the emplastrum containing 0.3%VE
With the preparation of the emplastrum containing 0.1%VE, VE oil 0.3g (VE content 0.5mg/mg) is added in volatile oil about 5.0g, i.e.,
?.(being 0.1g/g containing concentration, VE dosage is the 3% of volatile oil weight)
2.1.6 the preparation of the emplastrum containing 0.6%VE
With the preparation of the emplastrum containing 0.1%VE, VE oil 0.6g (VE content 0.5mg/mg) is added in volatile oil about 5.0g, i.e.,
?.(being 0.1g/g containing concentration, VE dosage is the 6% of volatile oil weight)
2.1.7 the preparation of the emplastrum containing 1%VE
With the preparation of the emplastrum containing 0.1%VE, VE oil 1.0g (VE content 0.5mg/mg) is added in volatile oil about 5.0g, i.e.,
?.(being 0.1g/g containing concentration, VE dosage is the 10% of volatile oil weight)
2.1.8 the preparation of the emplastrum containing 1.5%VE
With the preparation of the emplastrum containing 0.1%VE, VE oil 1.5g (VE content 0.5mg/mg) is added in volatile oil about 5.0g, i.e.,
?.(being 0.1g/g containing concentration, VE dosage is the 15% of volatile oil weight)
2.1.9 the preparation of blank protection emplastrum
It takes 1.1.1 lower prescription volatile oil spare, separately weighs lanolin 45.0g (36-42 DEG C of fusing point), set 60 DEG C of water-baths
Pot, water-bath to molten condition, taking-up are put to preference temperature, and volatile oil about 5.0g is added in matrix, is uniformly mixed, lets cool.It takes not
Each 1.5g of paste containing antioxidant, fills in blank patch, sealing, with fine aluminium bag pack to get.(being 0.1g/g containing concentration)
2.1.10 DINGHUIER QITIE reagent
20 patches, lot number: 121003
2.2 methods and result
2.2.1 test method
Above-mentioned sample is set in sealing clean container, is placed 5 days under the conditions of 60 DEG C, was sampled in 0,3,5 day, is measured, meter
It counts in stating index components retention rate.
2.2.2 test solution preparation and measurement
Emplastrum 0.25g is weighed, it is accurately weighed, it sets in stuffed conical flask, respectively accurate addition methanol 25ml, weighed weight,
Ultrasonic 45min, lets cool, then weighed weight, and the weight of less loss is supplied with methanol, is shaken up, and filtration takes subsequent filtrate to get test sample
Solution.It is appropriate to draw above-mentioned each test solution, miillpore filter filtration takes subsequent filtrate, as a result hplc determination is seen below
Table.
Comparison of the 17 antioxidant VE different amounts of table to the heated protecting effect of cinnaldehydrum
Comparison of the 18 antioxidant VE different amounts of table to the heated protecting effect of eugenol
Comparison of the 19 antioxidant VE different amounts of table to the heated protecting effect of Atisine chloride Atractydin
The results show that compared with the emplastrum without VE, cinnaldehydrum, fourth in 60 DEG C of heated 3,5 days different VE content emplastrums
Fragrant phenol, Atisine chloride Atractydin retain preferably, but VE is significantly affected the protecting effect of heterogeneity in emplastrum by VE content, it is excessively high or
The too low performance for being unfavorable for VE best protection effect, it is 0.15%~0.3% that wherein cinnaldehydrum, which corresponds to VE dosage in emplastrum, fourth
It is 0.1%~2.0% that fragrant phenol, which corresponds to VE dosage in emplastrum, and it is 0.10%~1.5% that Atisine chloride Atractydin, which corresponds to VE dosage in emplastrum, and 60
DEG C of heated 10 days test results shows, are retained containing cinnaldehydrum, eugenol, Atisine chloride Atractydin in 0.15%~0.3%VE emplastrum and are better than
Other VE dosage emplastrums.As a result prompt antioxidant VE Optimum (is equivalent to addition volatilization for the 0.15%~0.3% of emplastrum
The 1.5%~3.0% of oily weight), cinnaldehydrum, eugenol stability are significantly better than DINGHUIER QITIE in emplastrum.
3. the verifying of preparation stabilization measure: the accelerated stability test containing 0.2%VE, 0.3%VE patch
This research using cinnaldehydrum, eugenol, Atisine chloride Atractydin, Bronyl acetate retention rate as index, using simulation listing packet
Dress is fine aluminium bag hermetic package, investigates the accelerated stability containing 0.2%, 0.3%VE patch.
3.1 experimental material
3.1.1 the preparation of the emplastrum containing 0.2%VE
It takes 1.1.1 lower prescription volatile oil spare, separately weighs lanolin 135.0g (36-42 DEG C of fusing point), set 60 DEG C of water-baths
Pot, water-bath to molten condition, taking-up are put to preference temperature, and VE powder 0.3g is added in volatile oil about 15.0g, mixes with matrix
It is even, it lets cool.Take each 1.5g of paste of group containing VE, fill in blank patch, seal, with fine aluminium bag pack to get.(it is containing concentration
0.1g/g, VE dosage are the 2% of volatile oil weight)
3.1.2 the preparation of the emplastrum containing 0.3%VE
Be added with the preparation of the emplastrum containing 0.2%VE, in volatile oil about 15.0g VE powder 0.45g to get.(it is containing concentration
0.1g/g, VE dosage are the 3% of volatile oil weight)
3.1.3 the preparation of blank protection emplastrum
With the preparation of the emplastrum containing 0.2%VE, be not added in volatile oil about 15.0g VE to get.(it is 0.1g/g containing concentration,
Containing VE0%)
3.2 methods and result
3.2.1 test method
Above-mentioned sample is set in sealing clean container, is placed 6 months under the conditions of 30 DEG C, in 0,1,2,3, sampling in June, is surveyed
It is fixed, calculate These parameters ingredient retention rate.
3.2.2 test solution preparation and measurement
Emplastrum about 0.25g is weighed, it is accurately weighed, it sets in stuffed conical flask, accurate addition methanol 25ml, weighed heavy respectively
Amount, ultrasonic 45min is let cool, then weighed weight, and the weight of less loss is supplied with methanol, is shaken up, and is filtered, and takes subsequent filtrate to get for examination
Product solution.It is appropriate to draw above-mentioned each test solution, miillpore filter filtration takes subsequent filtrate, as a result hplc determination is seen below
Table.
3.3 experimental results and discussion:
20 accelerated stability test 1 of table, 2,3, retention results in June (cinnaldehydrum, eugenol, Atisine chloride Atractydin)
21 accelerated stability test 1 of table, 2,3, retention results in June (Bronyl acetate)
By cinnaldehydrum, eugenol, rhizoma atractylodis cellulose content in high effective liquid chromatography for measuring different time points preparation, as a result show
Show after accelerated test 6 months, cinnaldehydrum in blank protection patch, eugenol, Atisine chloride Atractydin retention rate be respectively 49.9%,
73.6%, 60.1%；In patch containing 0.3%VE cinnaldehydrum, eugenol, Atisine chloride Atractydin retention rate be respectively 68.3%, 89.9%,
78.9%, it is significantly improved.By the content of Bronyl acetate in capillary gas chromatography different time points preparation,
As the result is shown after accelerated test June, it is 88.1% that blank, which protects Bronyl acetate in patch, contains 0.2%VE, 0.3%VE
Its retention rate is respectively 95.3%, 92.5% in patch, prompts vitamin E significant to Bronyl acetate protective effect in patch.
The retention rate of four index components is evaluated by accelerated stability test, in terms of comprehensive, 0.15%~0.30%VE is used in emplastrum
Amount (be equivalent to and be added the 1.5%~3.0% of volatile oil weight) is suitable.
Specific embodiment
Embodiment 1 (preparation example of volatile oil)
Medicinal powder (middle powder) each 10kg for weighing fructus amomi, rhizoma atractylodis, cortex cinnamomi and cloves medicine is separately added into 15 times of amount water and impregnates
30 minutes, refluxing extraction under conditions of 100 DEG C；Volatile oil is collected, amomum fruit volatile oil 373.6g, Rhzoma Atractylodis Lanceae volatile oil are respectively obtained
135g, Cortex Cinnamomi volatile oil 212.12g and volatile clove oil 520g.Four kinds of obtained volatile oil seal respectively to be kept in dark place, and supplies
Following embodiments 2~7 use.
Embodiment 2
1, prescription: amomum fruit volatile oil 5.6g, Rhzoma Atractylodis Lanceae volatile oil 5.7g, Cortex Cinnamomi volatile oil 4.8g, volatile clove oil 7.8g.This
The total weight of four kinds of volatile oil is 23.9g in example.
2, prepare ointment: taking four kinds of volatile oil that the vitamin E of 0.359g is added, (vitamin E dosage is volatile oil weight
1.5%) it is uniformly mixed；Lanolin 215.1g (36-42 DEG C of fusing point) is weighed, is placed in 100 DEG C of water-baths, water-bath to molten condition,
Taking-up is put four containing the vitamin E kind volatile oil prepared by being added to 50 DEG C and is uniformly mixed to get ointment.
3, it prepares patch: ointment being coated in the leakage-proof ring of blank plaster while hot by 0.2g/ patch, after cooling, then is covered
Upper anti-stick layer, with fine aluminium bag sealing be protected from light pack to get.Wherein, the blank plaster is purchased from Shandong and laughs at health biotechnology
Co., Ltd, specification are as follows: empty veneer product is 7cm × 7cm, and the internal diameter of leakage-proof ring is 3.0cm, depth 0.2cm.
Embodiment 3
2, prescription: amomum fruit volatile oil 5.9g, Rhzoma Atractylodis Lanceae volatile oil 5.2g, Cortex Cinnamomi volatile oil 4.6g, volatile clove oil 8.0g.This
The total weight of four kinds of volatile oil is 23.7g in example.
2, prepare ointment: taking four kinds of volatile oil that the vitamin E of 0.427g is added, (vitamin E dosage is volatile oil weight
1.8%) it is uniformly mixed；Lanolin 213.3g (36-42 DEG C of fusing point) is weighed, is placed in 100 DEG C of water-baths, water-bath to molten condition,
Taking-up is put four containing the vitamin E kind volatile oil prepared by being added to 50 DEG C and is uniformly mixed to get ointment.
3, it prepares patch: ointment being coated in the leakage-proof ring of blank plaster while hot by 0.2g/ patch, after cooling, then is covered
Upper anti-stick layer, with fine aluminium bag sealing be protected from light pack to get.Wherein, the blank plaster is purchased from Shandong and laughs at health biotechnology
Co., Ltd, specification are as follows: empty veneer product is 7cm × 7cm, and the internal diameter of leakage-proof ring is 3.0cm, depth 0.2cm.
Embodiment 4
1, prescription: amomum fruit volatile oil 5.1g, Rhzoma Atractylodis Lanceae volatile oil 5.5g, Cortex Cinnamomi volatile oil 4.8g, volatile clove oil 8.4g.This
The total weight of four kinds of volatile oil is 23.8g in example.
2, prepare ointment: taking four kinds of volatile oil that the vitamin E of 0.476g is added, (vitamin E dosage is volatile oil weight
2%) it is uniformly mixed；Lanolin 214.2g (36-42 DEG C of fusing point) is weighed, 100 DEG C of water-baths are placed in, water-bath to molten condition takes
Four containing the vitamin E kind volatile oil prepared by being added to 50 DEG C is put out to be uniformly mixed to get ointment.
3, it prepares patch: ointment being coated in the leakage-proof ring of blank plaster while hot by 0.2g/ patch, after cooling, then is covered
Upper anti-stick layer, with fine aluminium bag sealing be protected from light pack to get.Wherein, the blank plaster is purchased from Shandong and laughs at health biotechnology
Co., Ltd, specification are as follows: empty veneer product is 7cm × 7cm, and the internal diameter of leakage-proof ring is 3.0cm, depth 0.2cm.
Embodiment 5
1, prescription: amomum fruit volatile oil 5.8g, Rhzoma Atractylodis Lanceae volatile oil 5.9g, Cortex Cinnamomi volatile oil 4.2g, volatile clove oil 7.7g.This
The total weight of four kinds of volatile oil is 23.6g in example.
2, prepare ointment: taking four kinds of volatile oil that the vitamin E of 0.543g is added, (vitamin E dosage is volatile oil weight
2.3%) it is uniformly mixed；Lanolin 212.4g (36-42 DEG C of fusing point) is weighed, is placed in 100 DEG C of water-baths, water-bath to molten condition,
Taking-up is put four containing the vitamin E kind volatile oil prepared by being added to 50 DEG C and is uniformly mixed to get ointment.
3, it prepares patch: ointment being coated in the leakage-proof ring of blank plaster while hot by 0.2g/ patch, after cooling, then is covered
Upper anti-stick layer, with fine aluminium bag sealing be protected from light pack to get.Wherein, the blank plaster is purchased from Shandong and laughs at health biotechnology
Co., Ltd, specification are as follows: empty veneer product is 7cm × 7cm, and the internal diameter of leakage-proof ring is 3.0cm, depth 0.2cm.
Embodiment 6
1, prescription: amomum fruit volatile oil 5.5g, Rhzoma Atractylodis Lanceae volatile oil 5.2g, Cortex Cinnamomi volatile oil 5.0g, volatile clove oil 8.1g.This
The total weight of four kinds of volatile oil is 23.8g in example.
2, prepare ointment: taking four kinds of volatile oil that the vitamin E of 0.595g is added, (vitamin E dosage is volatile oil weight
2.5%) it is uniformly mixed；Lanolin 214.2g (36-42 DEG C of fusing point) is weighed, is placed in 100 DEG C of water-baths, water-bath to molten condition,
Taking-up is put four containing the vitamin E kind volatile oil prepared by being added to 50 DEG C and is uniformly mixed to get ointment.
3, it prepares patch: ointment being coated in the leakage-proof ring of blank plaster while hot by 0.2g/ patch, after cooling, then is covered
Upper anti-stick layer, with fine aluminium bag sealing be protected from light pack to get.Wherein, the blank plaster is purchased from Shandong and laughs at health biotechnology
Co., Ltd, specification are as follows: empty veneer product is 7cm × 7cm, and the internal diameter of leakage-proof ring is 3.0cm, depth 0.2cm.
Embodiment 7
1, prescription: amomum fruit volatile oil 5.9g, Rhzoma Atractylodis Lanceae volatile oil 5.8g, Cortex Cinnamomi volatile oil 4.6g, volatile clove oil 7.4g.This
The total weight of four kinds of volatile oil is 23.7g in example.
2, prepare ointment: taking four kinds of volatile oil that the vitamin E of 0.711g is added, (vitamin E dosage is volatile oil weight
3%) it is uniformly mixed；Lanolin 213.3g (36-42 DEG C of fusing point) is weighed, 100 DEG C of water-baths are placed in, water-bath to molten condition takes
Four containing the vitamin E kind volatile oil prepared by being added to 50 DEG C is put out to be uniformly mixed to get ointment.
3, it prepares patch: ointment being coated in the leakage-proof ring of blank plaster while hot by 0.2g/ patch, after cooling, then is covered
Upper anti-stick layer, with fine aluminium bag sealing be protected from light pack to get.Wherein, the blank plaster is purchased from Shandong and laughs at health biotechnology
Co., Ltd, specification are as follows: empty veneer product is 7cm × 7cm, and the internal diameter of leakage-proof ring is 3.0cm, depth 0.2cm.
Claims (3)
1. a kind of patch for treating baby diarrhea, which includes having the blank plaster of leakage-proof ring and being coated on the leakproof
Ointment in circle, the ointment are made of effective component, vitamin E and matrix, wherein
The effective component is made of the volatile oil of following weight percent amount: amomum fruit volatile oil 20.5~25.2%, rhizoma atractylodis are waved
Hair oil 20.7~25.2%, Cortex Cinnamomi volatile oil 17.0~21.2%, volatile clove oil 29.1~35.4%；
The volatile oil for forming the effective component is made by following methods: taking fructus amomi, rhizoma atractylodis, cortex cinnamomi and the medicinal material powder of cloves respectively
End is added 15 times of amount water and impregnates 30 minutes, refluxing extraction under conditions of 100 DEG C；Volatile oil is collected, fructus amomi volatilization is respectively obtained
Oil, Rhzoma Atractylodis Lanceae volatile oil, Cortex Cinnamomi volatile oil and volatile clove oil；
The dosage of the vitamin E is the 1.5%~3.0% of effective component weight；
The matrix is lanolin.
2. a kind of patch for treating baby diarrhea according to claim 1, which is characterized in that the effective component by with
The volatile oil of lower weight percent amount forms: amomum fruit volatile oil 23.43%, Rhzoma Atractylodis Lanceae volatile oil 23.85%, Cortex Cinnamomi volatile oil
20.08%, volatile clove oil 32.64%.
3. a kind of patch for treating baby diarrhea according to claim 1 or 2, which is characterized in that the patch is by with lower section
Method is made:
(1) it takes the effective component that vitamin E is added and stirs evenly；
(2) matrix is taken, 100 DEG C of water-baths, water-bath to molten condition are placed in, taking-up is put to 50 DEG C, imitates Cheng Fen ︰ base by You
Matter=1 ︰ 9 mass ratio is added the effective component containing vitamin E obtained by step (1) and is uniformly mixed, and ointment is made；
(3) ointment is coated on while hot in the leakage-proof ring of blank plaster by the dosage of every patch 0.2g, after cooling, is covered anti-stick
Layer to get.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510670816.XA CN105126007B (en) | 2015-10-13 | 2015-10-13 | A kind of patch for treating baby diarrhea |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510670816.XA CN105126007B (en) | 2015-10-13 | 2015-10-13 | A kind of patch for treating baby diarrhea |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105126007A CN105126007A (en) | 2015-12-09 |
| CN105126007B true CN105126007B (en) | 2018-12-25 |
Family
ID=54711818
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510670816.XA Active CN105126007B (en) | 2015-10-13 | 2015-10-13 | A kind of patch for treating baby diarrhea |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105126007B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108619443A (en) * | 2018-05-23 | 2018-10-09 | 孙占军 | A kind of plaster of warm acupuncture point analgesic |
| CN113476489B (en) * | 2020-12-31 | 2022-10-11 | 成都中医药大学 | Ligusticum wallichii volatile oil plaster for treating dysmenorrhea as well as preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631386A (en) * | 2003-12-25 | 2005-06-29 | 胡义保 | Navel plastered film for treating infantile diarrhea |
| CN102451448A (en) * | 2010-10-19 | 2012-05-16 | 叶军伟 | Preparation method for costusroot, clove and cassia bark powder |
| CN103054990A (en) * | 2012-12-26 | 2013-04-24 | 柳州市中医院 | Traditional Chinese medicine for treating functional abdominal pain in children and preparation method thereof |
-
2015
- 2015-10-13 CN CN201510670816.XA patent/CN105126007B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631386A (en) * | 2003-12-25 | 2005-06-29 | 胡义保 | Navel plastered film for treating infantile diarrhea |
| CN102451448A (en) * | 2010-10-19 | 2012-05-16 | 叶军伟 | Preparation method for costusroot, clove and cassia bark powder |
| CN103054990A (en) * | 2012-12-26 | 2013-04-24 | 柳州市中医院 | Traditional Chinese medicine for treating functional abdominal pain in children and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| "丁桂苍倍膏贴脐治疗寒湿泄泻96例";宋新堂等;《国医论坛》;19981231;第13卷(第3期);39 * |
| "丁香酚、桂皮醛不同存在状态下的稳定性影响因素研究";强皎等;《中药新药与临床药理》;20140131;第25卷(第1期);第89页、第90页左栏第1、5段、右栏第2段、第92页左栏第1段 * |
| "急性腹泻患儿血清维生素E的变化";王琳琳等;《中国现代医学杂志》;19980630;第8卷(第6期);第6页右栏第2、3段 * |
| "泻克星敷脐治疗小儿腹泻的临床观察";胡义保等;《中国医药学报》;19971231;第12卷(第2期);34-36 * |
| "脐疗散治疗小儿腹泻114例";刘绍芬等;《中医外治杂志》;19971231(第4期);第13页左栏第3段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105126007A (en) | 2015-12-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103766908B (en) | A kind of health Chu chrysanthemum buccal tablet and production method thereof | |
| JP2001514650A (en) | Essential oil composition | |
| EP3193898A2 (en) | Eurycoma longifolia extract and its use in enhancing and/or stimulating immune system | |
| CN109568447A (en) | Improve the Chinese medicine composition and its preparation method and application of sleep quality | |
| CN105126007B (en) | A kind of patch for treating baby diarrhea | |
| CN105288418B (en) | A kind of micro emulsion gel paste and preparation method thereof for treating baby diarrhea | |
| CN103536757B (en) | Traditional Chinese medicine composition having effects of tonifying kidney, replenishing essence, tonifying qi, nourishing blood and consolidating basis, and preparation method and application thereof | |
| CN102861193B (en) | Traditional Chinese medicine composition for treating gastric cancer | |
| CN104491797A (en) | Use of traditional Chinese medicine preparation in preparation of drug for treating intestinal parasites | |
| CN105169205B (en) | A kind of navel paster for treating baby diarrhea | |
| CN107050056A (en) | A kind of donkey-hide gelatin composition and preparation method and application | |
| CN104474112A (en) | Traditional Chinese medicine for treating urinary calculus and preparation method of traditional Chinese medicine | |
| CN108113953A (en) | A kind of dendrobium candidum honey composite extract with moisture-keeping efficacy and preparation method thereof and the application in skin care item | |
| CN110721256B (en) | Traditional Chinese medicine composition for preventing and treating teniasis of laying hens and preparation method thereof | |
| CN105288501A (en) | Traditional Chinese medicine composition containing folium artemisiae argyi and treating obesity | |
| CN113413445B (en) | Composition and preparation method and application thereof | |
| CN109745422A (en) | A kind of Chinese medicine composition and preparation method thereof for treating kidney-yang deficiency | |
| CN109248193A (en) | A kind of pharmaceutical composition and preparation method thereof for snoring | |
| CN105853582A (en) | Folium artemisiae argyi containing medicine preparation for treating cholelithiasis and preparation method thereof | |
| CN118416189B (en) | Medicinal and edible composition and preparation method and application thereof | |
| JP6708916B1 (en) | Zhuang family pharmaceutical composition for regulating replenishment and method for preparing the same | |
| CN1546134A (en) | Prescription and preparing process and preparation of Wugen capsule | |
| CN105288498A (en) | Method for preparing folium artemisiae argyi-contained traditional Chinese medicine preparation capable of treating obesity | |
| KR101366230B1 (en) | Use of treating and relaxing of snore and sleep apnea syndrome of mistletoe and composition comprising thereof as active ingredient | |
| CN106491706A (en) | Ginkgo leaf capsule of red sage root |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |