CN105125526A - Dimethyl sulfoxide antibacterial liquid - Google Patents
Dimethyl sulfoxide antibacterial liquid Download PDFInfo
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- CN105125526A CN105125526A CN201510505998.5A CN201510505998A CN105125526A CN 105125526 A CN105125526 A CN 105125526A CN 201510505998 A CN201510505998 A CN 201510505998A CN 105125526 A CN105125526 A CN 105125526A
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- dimethyl sulfoxide
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- antibacterial liquid
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses dimethyl sulfoxide antibacterial liquid and belongs to the technical field of biology. The dimethyl sulfoxide antibacterial liquid is a mixed solution composed of dimethyl sulfoxide, ethyl alcohol and water, which are the same in volume, has an inhibiting effect on escherichia coli or staphylococcus aureus, and can be combined with bleeding stopping cotton fibre to be used for resisting bacteria of wounds, for example, the dimethyl sulfoxide antibacterial liquid is prepared into band-aids and other products.
Description
Technical field
The present invention relates to a kind of antibacterial liquid, be specifically related to the miscible fluid be made up of dimethyl sulfoxide and ethanol and water, belong to biological technical field.
Background technology
Dimethyl sulfoxide (dimethylsulfoxide, DMSO) is colourless liquid, almost odorless, slightly bitterness, is a kind of important polar organic solvent, and it can dissolve each other with many organic solvents and water.Medicinal DMSO is mainly used in medicine and intermediate synthesis reaction solvent, the carrier etc. of medicine solvent and medicine, DMSO can dissolve multi-medicament, and increase its permeability, thus drug absorption can be increased and improve curative effect, and itself toxicity is very low, also there is antiinflammatory, pain relieving, the effect that blood circulation promoting and wound healing, diuresis, pin are quiet, have the title of " catholicon ", the active component of Chang Zuowei analgesic drug product makes an addition among medicine.In addition, there are some researches show DMSO to neurodermatitis, psoriasis, arthritis, rheumatoid, sprain, lumbar muscle strain, burn etc. all have curative effect.DMSO is also a kind of permeability protective agent, can reduce cell freezing point, reduces the formation of ice crystal, alleviates radical pair primary cellular defect, changes biomembrane to the permeability of electrolyte, medicine, poisonous substance and metabolite.
A lot of at research report medically abroad, China has carried out comprehensive toxicity by Shenyang Pharmacy College, Beijing institute of materia medica, Chinese drug detection center etc. in the sixties and to have checked and pathological anatomy proves: half quantity LG50 value of the DMSO of China is (22.4 ± 1.4) g/kg, the 21.4g/kg reported with foreign literature is basically identical, belong to nontoxic, conform to pathological anatomy finding.Beijing drug research sarcolycin used, the first-class drug research of B518, C6323, N sarcolycin used, tumor local, obtains good curative effect, obvious Tumor suppression growth.When zoopery, detect through dissecting, local drug concentration is higher than other drug concentration 2 ~ 8 times.Foreign study thinks that cancerous cell has one deck cutin protecting film, and hinder medicine to enter, DMSO has the solution pervasion ability to cutin, so can improve curative effect.
Clinic trial is carried out as far back as 160 medical institutions of BJ Union Hospital etc. the sixties, and offer thematic outpatient service in Benxi, have accumulated mass data, facts have proved neurodermatitis, psoriasis, arthritis, bursitis, folliculitis, rheumatoid, otitis media, rhinitis, adnexitis, have a toothache, herpes zoster, hemorrhoid, sprain, lumbar muscle strain, burn, wound etc. all have curative effect.Widely use in the medicine for external use such as GUYOULING, beriberi medicament, topsyne ointment of current production and the external preparation of various big hospital.Chinese Academy of Sciences veterinary institute DMSO dissolves " Paasche " and treats equine infectious anemia and parasitic disease.Particularly in Chinese medicine extraction preparation, improve useful constituent content, improve drug effect.
The research of DMSO in clinical medicine was once once slowed down, 2012, Capriotti and Capriotti [4] finds, DMSO plays antiinflammatory action by scavenging free radicals, made the attention that the medicinal characteristic of DMSO obtains again.Find in the research of plant extract antibacterial activity, DMSO has bacteriostasis when higher concentration.The people such as pond crystal choose pathogenic bacterium through experiment: escherichia coli, dysentery bacterium, and the Candida albicans in staphylococcus aureus and fungus is object of study, probes into the antagonistic property of DMSO.Result of study shows that DMSO has good fungistatic effect, and therefore it may be applied as a kind of new bacteriostatic agent.There are some researches show, the growth of DMSO to some fungi or coccus has certain inhibitory action, sprouts namely comparatively obvious to the inhibitory action of fungal attack power especially, and there is docs-effect dependency to fungal spore and germ tube.Therefore, DMSO has positive clinical value in anti-fungal infection.But, clinical infection fungal species is various, physiological characteristics and growth characteristics different, also be only tip of the iceberg to the research of fungus and antibacterial in the past, and the researches to antibacterial, also only cellular level is rested on to the research of some fungi, the research dynamics of DMSO to fungus and antibacterial should be strengthened from now on, the mechanism of its antimicrobial mechanism and antifungal spore and germ tube sprouting is disclosed, for the clinical practice of DMSO provides foundation at molecule and gene level.The research of the application aspect of DMSO in the correlational study and treatment bacterial infection disease of anti-bacteria toxicity related gene, Pathogenicity of Bacteria is not yet paid attention to.Northwest University's application number be 201410102404.1 patent application to propose volumetric concentration percentage ratio be that the dimethyl sulfoxide liquid of 0.01%-10% is controlling the application in bacteriological infection, antibacterial is gram negative bacteria.
In routine work and life, skin injury right and wrong are usually shown in.In the daily outpatient service of hospital, common skin injury has scratch, incision, dampens, stabs and crush injury etc., although wound is very little sometimes, if process bad, probably causes suppuration, makes wound be not easy healing and affect living and studying.In limited instances, and some special population treatment of wounds are improper also can cause septicemia, tetanus etc., even threat to life.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of dimethyl sulfoxide antibacterial liquid:
In the present invention, dimethyl sulfoxide is controlling the application in bacteriological infection, and antibacterial is escherichia coli or staphylococcus aureus.
A kind of dimethyl sulfoxide antibacterial liquid, the miscible solution be made up of the dimethyl sulfoxide of equal volume, ethanol, water, namely antibacterial liquid composition is dimethyl sulfoxide, ethanol, water, and the volume ratio of three is 1:1:1.
Compared with prior art, beneficial effect of the present invention is, the water of dimethyl sulfoxide and ethanol mixes miscible solution, has inhibitory action, have clinical value to mankind's common pathogen escherichia coli and staphylococcus aureus.This antibacterial in conjunction with hemostatic cotton fibre etc. for Wound antibiotic, such as, can be made into the products such as adhesive bandage.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in detail, also described technical problem, principle and beneficial effect that technical solution of the present invention solves simultaneously.
The present embodiment is with the bacteriostasis property of the described dimethyl sulfoxide antibacterial liquid of antibacterial around-France test.
(1) culture medium is prepared.Get 1000mL beaker, with 1000mL hot water dissolving 18g nutrient meat powder and 1.5g agar powder, average mark is loaded in 5 250mL conical flasks.
(2) sterilizing.By the culture medium 100 μ L prepared, 1000 μ L suction pipette heads load rifle head box, with 121 DEG C of high pressure steam sterilization 10min.
(3) inoculation and cultivation.The culture medium of sterilizing is poured into respectively in 20 culture dishs, cooling.Escherichia coli original bacteria liquid is mixed, gets 50 μ L original bacteria liquid with micropipettor, be inoculated in flat board with 50 μ L sterilized water, use spreader even spread.Be 1cm filter paper by diameter, be soaked in miscible fluid sample 1#, 10min in 2#, 3# and 4#75% alcohol liquid.The filter paper soaked in different blended solution is placed on the culture dish of inoculation respectively.Culture dish is positioned in constant-temperature table, cultivates 24h in 37 DEG C.
(4) observe and measure.Culture dish is taken out from constant-temperature table, observes and measure bacterial restrain radius.The experimental technique of staphylococcus aureus is the same.
(5) date processing.The data obtained is aggregated in Experiment Data Records, in table 1.
Table 1, DMSO-alcohol-water miscible fluid bacteriostasis property compare (antibacterial around-France)
From the results shown in Table 1, the DMSO miscible solution of variable concentrations all creates bacterial restrain.Through relative analysis find, 1# sample and its biocidal property of DMSO miscible fluid proposed by the invention better.DMSO miscible fluid to the inhibiting mechanism of action of antibacterial may with following factor about: (1) can cause the change of bacterial cell membrane protein conformation, and the Bound moisture displaced wherein forms loose structure, enhances membrane passage; (2) change of inducing cell activity, makes its competitive inhibition acetaminophen thus causes damage and the death of cell; (3) eubacteria surface of cell membrane protein channel is changed active; (4) reduce bacterial cell transmembrane potential, increase cell membrane satisfied fatty acid ratio, increase permeability of cell membrane, thus the growth of antibacterial is suppressed, even dead.The biocidal property of the miscible solution containing DMSO is better than medical alcohol.This may be due to following factor: the antibiotic property that (1) DMSO self has.(2) DMSO has dissolving and the penetrating power of Stratum Corneum compared to ethanol.(3) DMSO can scavenging free radicals to have scholar to find, thus can play antiinflammatory action.
This antibacterial in conjunction with hemostatic cotton fibre etc. for Wound antibiotic, such as, can be made into the products such as adhesive bandage.
The above; be only the specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any people being familiar with this technology is in the technical scope disclosed by the present invention; the conversion and replacement expected can be understood; all should be encompassed in and of the present inventionly comprise within scope, therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Claims (1)
1. a dimethyl sulfoxide antibacterial liquid, is characterized in that, its composition is dimethyl sulfoxide, ethanol, water, and the volume ratio of three is 1:1:1; Antibacterial is escherichia coli or staphylococcus aureus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510505998.5A CN105125526A (en) | 2015-08-17 | 2015-08-17 | Dimethyl sulfoxide antibacterial liquid |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510505998.5A CN105125526A (en) | 2015-08-17 | 2015-08-17 | Dimethyl sulfoxide antibacterial liquid |
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| CN105125526A true CN105125526A (en) | 2015-12-09 |
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| CN201510505998.5A Pending CN105125526A (en) | 2015-08-17 | 2015-08-17 | Dimethyl sulfoxide antibacterial liquid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022143984A1 (en) * | 2020-12-31 | 2022-07-07 | 邓亚琴 | Pharmaceutical composition for treating cancer, preparation method therefor and use thereof |
-
2015
- 2015-08-17 CN CN201510505998.5A patent/CN105125526A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 池水晶等: "二甲基亚砜的体外抗菌活性研究", 《中国热带医学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022143984A1 (en) * | 2020-12-31 | 2022-07-07 | 邓亚琴 | Pharmaceutical composition for treating cancer, preparation method therefor and use thereof |
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