CN105124602A - Composite nutrient supplemental agent with high-activity lactic acid bacteria and preparation method of composite nutrient supplemental agent - Google Patents
Composite nutrient supplemental agent with high-activity lactic acid bacteria and preparation method of composite nutrient supplemental agent Download PDFInfo
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- CN105124602A CN105124602A CN201510547236.1A CN201510547236A CN105124602A CN 105124602 A CN105124602 A CN 105124602A CN 201510547236 A CN201510547236 A CN 201510547236A CN 105124602 A CN105124602 A CN 105124602A
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- lactic acid
- acid bacteria
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- composite nutrient
- supplemental agent
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 74
- 241000894006 Bacteria Species 0.000 title claims abstract description 39
- 239000004310 lactic acid Substances 0.000 title claims abstract description 37
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 37
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 24
- 230000000694 effects Effects 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims description 14
- 235000015097 nutrients Nutrition 0.000 title abstract description 10
- 239000002131 composite material Substances 0.000 title abstract 8
- 230000000153 supplemental effect Effects 0.000 title abstract 8
- 244000068988 Glycine max Species 0.000 claims abstract description 23
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 23
- 238000001694 spray drying Methods 0.000 claims abstract description 6
- 238000000855 fermentation Methods 0.000 claims description 18
- 230000004151 fermentation Effects 0.000 claims description 18
- 235000016709 nutrition Nutrition 0.000 claims description 15
- 150000001875 compounds Chemical class 0.000 claims description 13
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 12
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 9
- 239000000084 colloidal system Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 4
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 4
- 244000199866 Lactobacillus casei Species 0.000 claims description 4
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 4
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 4
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 4
- 229940017800 lactobacillus casei Drugs 0.000 claims description 4
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 3
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 3
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 3
- 239000000839 emulsion Substances 0.000 claims description 3
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 3
- 238000002663 nebulization Methods 0.000 claims description 3
- 239000012531 culture fluid Substances 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 238000011218 seed culture Methods 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 abstract description 7
- 150000001413 amino acids Chemical class 0.000 abstract description 4
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 abstract description 4
- 235000008696 isoflavones Nutrition 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract description 3
- 210000000936 intestine Anatomy 0.000 abstract description 3
- 239000011707 mineral Substances 0.000 abstract description 3
- 239000006041 probiotic Substances 0.000 abstract description 3
- 235000018291 probiotics Nutrition 0.000 abstract description 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 206010010774 Constipation Diseases 0.000 abstract description 2
- 239000000835 fiber Substances 0.000 abstract description 2
- 229920001542 oligosaccharide Polymers 0.000 abstract description 2
- 150000002482 oligosaccharides Chemical class 0.000 abstract description 2
- 239000006227 byproduct Substances 0.000 abstract 2
- 101710154606 Hemagglutinin Proteins 0.000 abstract 1
- 101710093908 Outer capsid protein VP4 Proteins 0.000 abstract 1
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 abstract 1
- 101710176177 Protein A56 Proteins 0.000 abstract 1
- 229940122618 Trypsin inhibitor Drugs 0.000 abstract 1
- 101710162629 Trypsin inhibitor Proteins 0.000 abstract 1
- 230000000433 anti-nutritional effect Effects 0.000 abstract 1
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract 1
- 239000000185 hemagglutinin Substances 0.000 abstract 1
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 239000002753 trypsin inhibitor Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 5
- 235000013305 food Nutrition 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 108010073771 Soybean Proteins Proteins 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 150000002515 isoflavone derivatives Chemical class 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 235000019710 soybean protein Nutrition 0.000 description 3
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 235000013618 yogurt Nutrition 0.000 description 2
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000006052 feed supplement Substances 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000000822 natural killer cell Anatomy 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a composite nutrient supplemental agent which is prepared by utilizing oil making by-product cake dregs as raw materials and utilizing a modern bioengineering technology and a spray drying technology and contains components of lactic acid bacteria, oligosaccharide and the like. According to the composite nutrient supplemental agent disclosed by the invention, anti-nutritional factors of a trypsin inhibitor, hemagglutinin and the like in soybeans are eliminated, functional components of soybean isoflavone, soybean fibers and the like are reserved, the composite nutrient supplemental agent has richer effective components such as soybean peptides, amino acid and mineral substances, and the viable count of the lactic acid bacteria is not less than 1*10<9>cfu/g. The composite nutrient supplemental agent disclosed by the invention not only provides nutrient substances required by the daily life of human bodies, but also contains a living bacterium agent of the lactic acid bacteria, so that a probiotics group in intestinal tracts of the human bodies can be consolidated or improved, the composite nutrient supplemental agent has the effect of loosening bowel to relieve constipation, and the intestine health of the human bodies can be maintained. According to the composite nutrient supplemental agent disclosed by the invention, the comprehensive utilization added value of the cake dregs is increased, and the deep processing industry chain of the oil making by-product cake dregs is extended.
Description
Technical field:
The present invention relates to and utilize grain and oil accessory substance dregs of beans or soya-bean cake to prepare product and the preparation method of high-activity lactic acid bacteria compound nutritional accessory agent for raw material, belong to bioengineering field.
Background technology:
Dregs of beans is the accessory substance after soybean peeling, compressing tablet, organic solvent lixiviate grease; Soya-bean cake is soybean peeling, through screw press or stupidly press the accessory substance after oil.Cake protein matter content after liquefaction is high, and generally at 40%-50%, essential amino acid kind is many, and amino acid ratio is comparatively reasonable, and containing more nicotinic acid and pantothenic acid, content of choline is up to 2200mg-2800mg/kg.In addition, also containing several functions compositions such as soyabean oligosaccharides, isoflavones, soybean dietary fibers.At present, China's dregs of beans annual production is at about 5,400 ten thousand tons, except the dregs of beans of small part low-temperature cold pressing soya-bean cake or low sex change is used for except the food such as food service industry production soybean protein isolate, FSPC and instant bean, most dregs of beans or soya-bean cake are used for feedstuff industry.But owing to carrying to soybean protein destruction in various degree in oily process, cause protein sex change to a certain degree in dregs of beans or soya-bean cake, thus human or animal is unfavorable for absorbing soybean protein after directly taking in.
Lactic acid bacteria is the probio of current humans and animals enteron aisle of generally acknowledging.Lactic acid bacteria can produce acetic acid, lactic acid, hydrogen peroxide and other antibacterial substance in enteron aisle, thus can suppress the pathogen in enteron aisle.Meanwhile, the lactic acid bacteria of adhesion can stimulate the host immune system of human or animal, strengthens host body immunity.Especially can activating macrophage and NK cell for human body, increase the activity of immunoglobulin (Ig), TNF, reduce risk of developing cancer.In addition, the generation of cholesterol can also be suppressed, promote the synthesis of B family vitamin, promote that intestines peristalsis prevents constipation from occurring, strengthen host's resistance against diseases.The lactic acid bacterias such as lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum are the conventional prebiotic bacterial classifications of lactic acid bacteria, but in the market only for for Yoghourt, Production of Soybean Yoghurt, and mainly with liquid form storage, the bacterial classification time-to-live is shorter, need refrigeration, holding conditions is limited.The solid-state storage procedures of the probios such as lactic acid bacteria is the most effective with freeze-drying at present, but the freeze-drying method consumption energy is large, production cost is high, is applicable to medicine preparation more.
Summary of the invention
The present invention is directed to the problem that in lactic acid bacteria probio liquid environment, the time-to-live is short, freeze-dried products cost is high, and the problem that sex change grouts digestibility is low, propose preparation method of a kind of high-activity lactic acid bacteria compound nutritional accessory agent and products thereof, meet prepare probiotic food low cost, high activity requirement.
The present invention includes following steps:
(1) high concentration dregs of beans or soya-bean cake fermentation medium load fermentation tank, and real tank sterilizing 30min, is cooled to 37 DEG C ~ 38 DEG C;
(2) to fermentation tank aseptic access lactic acid bacteria seed culture fluid, open and stir 10rpm ~ 100rpm, tank body pressurize 0.1MPa ~ 0.15MPa, cultivate 24h ~ 72h for 37 DEG C ~ 38 DEG C;
(3) fermentation ends, zymotic fluid crosses colloid mill 1 ~ 2 time;
(4) fermentation liquor low temperature spray drying, obtains high-activity lactic acid bacteria compound nutritional accessory agent.
Step (1) middle and high concentration dregs of beans or soya-bean cake concentration 10% ~ 15%, cross colloid mill and make emulsion before fermentation.
In step (2), lactic acid bacteria bacterial classification used comprises the lactic acid bacterias such as Lactobacillus plantarum, lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, Lactobacillus casei.
Fermentation ends in step (3), zymotic fluid crosses colloid mill 1 ~ 2 time.
In step (4), zymotic fluid low temperature spray drying condition is: cold nebulization tower feed liquid EAT 115 DEG C ~ 125 DEG C, discharging temperature of outgoing air 55 DEG C ~ 70 DEG C, temperature 70 C ~ 85 DEG C in tower.
In step (4), made spray dried powder viable lactic acid bacteria counting is not less than 1x10
9cFU/g.
This product take grouts as compound nutritional accessory agent prepared by raw material, and be rich in active ingredient and the functional components such as isoflavones, fibre and soya such as Soybean Peptide, amino acid, mineral matter, contained viable lactic acid bacteria numerical value is high, is not less than 1 × 10
9cfu/g.This product not only for human body provide every day desired nutritional material, and human body intestinal canal beneficial flora be consolidated or be improved to contained viable lactic acid bacteria agent can, relaxes bowel, and makes human body keep " intestines " healthy.Probiotics viable bacteria preparation prepared by relative employing freeze drying, this product facility small investment, preparation cost are low, product yield is high.Relative liquid lactic acid bacteria preparation, this product can keep lactic bacteria activity for more time, extends commodity shelf life.This product improves grouts comprehensive utilization added value, extends liquefaction accessory substance grouts process deeply industry chain.Namely this product can be used as food nutrition accessory agent, also can be used as high-grade feed supplement and uses.
Accompanying drawing explanation
Fig. 1 production technological process of the present invention
Detailed description of the invention
Detailed description of the invention:
Fermentation medium is prepared: adopt high speed disintegrator to pulverize below dregs of beans or soya-bean cake to 40 order, add water and be mixed with grouts concentration 10% ~ 15%, add auxiliary ingredients, after stirring evenly, liquid grouts are crossed colloid mill and are made emulsion by soaking at room temperature 20 minutes, load 100L fermentation tank, loadings 50L, real tank sterilizing 30min, is cooled to 37 DEG C ~ 38 DEG C.
Prepared by seed liquor: by the MRS fluid nutrient medium after the lactic acid bacteria access sterilizings such as activation Lactobacillus plantarum, lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, Lactobacillus casei, cultivate 24h ~ 48h for 37 DEG C.
Fermented and cultured: inject ripe seed liquor under aseptic condition in fermentation tank, opens and stirs 10rpm ~ 100rpm, pass into filtrated air immediately, tank body pressurize 0.1MPa ~ 0.15MPa, cultivates 24h ~ 72h for 37 DEG C ~ 38 DEG C.
Fermentation ends, zymotic fluid crosses colloid mill 1 ~ 2 time.
Zymotic fluid carries out low temperature spray drying, cold nebulization tower feed liquid EAT 115 DEG C ~ 125 DEG C, discharging temperature of outgoing air 55 DEG C ~ 70 DEG C, temperature 70 C ~ 85 DEG C in tower.
After testing, products made thereby is rich in Soybean Peptide, amino acid, mineral matter and soybean cellulose, isoflavones etc., and viable lactic acid bacteria counting is not less than 1x10
9cFU/g.
Claims (7)
1. high-activity lactic acid bacteria compound nutritional accessory agent and preparation method thereof, is characterized in that: (1) high concentration dregs of beans or soya-bean cake fermentation medium load fermentation tank, and real tank sterilizing 30min, is cooled to 37 DEG C ~ 38 DEG C; (2) to fermentation tank aseptic access lactic acid bacteria seed culture fluid, open and stir 10rpm ~ 100rpm, tank body pressurize 0.1MPa ~ 0.15MPa, cultivate 24h ~ 72h for 37 DEG C ~ 38 DEG C; (3) fermentation ends, zymotic fluid crosses colloid mill 1 ~ 2 time; (4) fermentation liquor low temperature spray drying, obtains high-activity lactic acid bacteria compound nutritional accessory agent.
2. a kind of high-activity lactic acid bacteria compound nutritional accessory agent according to right 1 and preparation method thereof, is characterized in that: high concentration dregs of beans or soya-bean cake concentration 10% ~ 15%, crosses colloid mill and make emulsion before fermentation.
3. a kind of high-activity lactic acid bacteria compound nutritional accessory agent according to right 1 and preparation method thereof, is characterized in that: lactic acid bacteria bacterial classification used comprises the lactic acid bacterias such as Lactobacillus plantarum, lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, Lactobacillus casei.
4. a kind of high-activity lactic acid bacteria compound nutritional accessory agent and preparation method thereof according to right 1, it is characterized in that: fermentation ends, zymotic fluid crosses colloid mill 1 ~ 2 time.
5. a kind of high-activity lactic acid bacteria compound nutritional accessory agent and preparation method thereof according to right 1, it is characterized in that: zymotic fluid low temperature spray drying condition is: cold nebulization tower feed liquid EAT 115 DEG C ~ 125 DEG C, discharging temperature of outgoing air 55 DEG C ~ 70 DEG C, temperature 70 C ~ 85 DEG C in tower.
6. a kind of high-activity lactic acid bacteria compound nutritional accessory agent and preparation method thereof according to right 1, is characterized in that: made spray dried powder viable lactic acid bacteria counting is not less than 1x10
9cFU/g.
7. the high-activity lactic acid bacteria compound nutritional accessory agent that method described arbitrarily in claim 1-6 obtains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510547236.1A CN105124602A (en) | 2015-08-31 | 2015-08-31 | Composite nutrient supplemental agent with high-activity lactic acid bacteria and preparation method of composite nutrient supplemental agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510547236.1A CN105124602A (en) | 2015-08-31 | 2015-08-31 | Composite nutrient supplemental agent with high-activity lactic acid bacteria and preparation method of composite nutrient supplemental agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105124602A true CN105124602A (en) | 2015-12-09 |
Family
ID=54710479
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510547236.1A Pending CN105124602A (en) | 2015-08-31 | 2015-08-31 | Composite nutrient supplemental agent with high-activity lactic acid bacteria and preparation method of composite nutrient supplemental agent |
Country Status (1)
| Country | Link |
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| CN (1) | CN105124602A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105647808A (en) * | 2016-02-25 | 2016-06-08 | 福建省绿麒食品胶体有限公司 | Method for preserving Lactobacillus bulgaricus with agaro-oligosaccharide used as protective agent |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003047422A (en) * | 2001-07-31 | 2003-02-18 | Shizen Kaiki Kenkyusho:Kk | Lactic acid fermented food of whole soybean flour, bean- curd refuse and grifola frondosa, and method for producing the same |
| CN103283974A (en) * | 2013-03-27 | 2013-09-11 | 广州格拉姆生物科技有限公司 | A method for producing a probiotic agent of live Lactobacillus bulgaricus |
-
2015
- 2015-08-31 CN CN201510547236.1A patent/CN105124602A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003047422A (en) * | 2001-07-31 | 2003-02-18 | Shizen Kaiki Kenkyusho:Kk | Lactic acid fermented food of whole soybean flour, bean- curd refuse and grifola frondosa, and method for producing the same |
| CN103283974A (en) * | 2013-03-27 | 2013-09-11 | 广州格拉姆生物科技有限公司 | A method for producing a probiotic agent of live Lactobacillus bulgaricus |
Non-Patent Citations (1)
| Title |
|---|
| 郑裴，等: "植物乳杆菌发酵豆粕及其抗营养因子的研究", 《安徽农学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105647808A (en) * | 2016-02-25 | 2016-06-08 | 福建省绿麒食品胶体有限公司 | Method for preserving Lactobacillus bulgaricus with agaro-oligosaccharide used as protective agent |
| CN105647808B (en) * | 2016-02-25 | 2019-09-27 | 福建省绿麒食品胶体有限公司 | A kind of method of agaropectin oligose as protective agent preservation lactobacillus bulgaricus |
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