CN105123624A - Method for controlling pomacea canaliculata by utilizing medicinal whitmania pigra - Google Patents

Method for controlling pomacea canaliculata by utilizing medicinal whitmania pigra Download PDF

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Publication number
CN105123624A
CN105123624A CN201510422298.XA CN201510422298A CN105123624A CN 105123624 A CN105123624 A CN 105123624A CN 201510422298 A CN201510422298 A CN 201510422298A CN 105123624 A CN105123624 A CN 105123624A
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China
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pomacea canaliculata
leech
golden thread
eurysome golden
thread leech
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朱丽霞
陈清森
章家恩
王俊橙
胡俊西
张泽宏
温华榜
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Minnan Normal University
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Minnan Normal University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

Abstract

The invention discloses a method for controlling pomacea canaliculata by utilizing medicinal whitmania pigra. The method includes that: the medicinal whitmania pigra is used as an object; a mixing culture and killing experiment of the pomacea canaliculata and the medicinal whitmania pigra, a weight gain experiment of the medicinal whitmania pigra, and a difference contrast experiment of predation of the medicinal whitmania pigra on male and female individuals of the pomacea canaliculata are performed based on life habits that the medicinal whitmania pigra feeds on sap sucked from mollusks such as viviparid, clam, and corbicula fluminea;the difference contrast experiment of predation of the medicinal whitmania pigra on the male and female individuals of the pomacea canaliculata shows that: when the predation number difference of the medicinal whitmania pigra fluctuates between 0 and 0.4, the predation of the medicinal whitmania pigra on the pomacea canaliculata has no gender difference; when the number and the density of the pomacea canaliculata are large, the number ratio of the the pomacea canaliculata to the medicinal whitmania pigra is more than 3 : 1, the pomacea canaliculata eats dead bodies of the medicinal whitmania pigra, but in this environment, the accumulation predation number of the medicinal whitmania pigra on the pomacea canaliculata reaches maximum, and it verifies that the certain control effect of the medicinal whitmania pigra on the pomacea canaliculata can be achieved.

Description

A kind of medicinal eurysome golden thread leech is to the preventing control method of Pomacea canaliculata
Technical field
The present invention relates to a kind of method of killing adult Pomacea canaliculata, especially a kind of medicinal eurysome golden thread leech is to the preventing control method of Pomacea canaliculata.
Background technology
Pomacea canaliculata, has another name called Ampullaria gigas, apple spiral shell, and amphibious freshwater shellfish software is biological, classification belongs to Mollusca, Gastropoda, Mesogastropoda, Ampullariidae, bottle spiral shell genus, belongs to Perenniporia martius kind; Originate in South America Amazon River basin, after the eighties in 20th century, Pomacea canaliculata is incorporated into the countries such as Japan, Philippine, Vietnam, Thailand gradually as a kind of food; 1981, first Pomacea canaliculata was introduced China Guangdong by as a kind of food too, after 1984, widely cultivate in Guangdong, and be diffused into rapidly the ground such as Guangxi, Fujian, Hainan, Zhejiang, Shanghai, Jiangsu, rear because of Pomacea canaliculata food flavor not good, and by abandon in field; Due to blind import and mismanagement, current Pomacea canaliculata causes disaster in south China many provinces outburst, and becomes the pernicious aquatic animal of China and other Asian countries's hazard rice and other water plants, is described as " paddy rice killer "; Pomacea canaliculata fits natural disposition by force, and like pure and fresh in water quality, live in the fresh water of foodstuff abundance, multigroup collection inhabits phytal zone, or is adsorbed on water plants cauline leaf; Be suitable for growing under water temperature is the condition of 25 ~ 32 DEG C, water temperature growth more than 35 DEG C obviously declines, and the highest critical water temperature of surviving is 45 DEG C, and minimum critical water temperature is 5 DEG C; Pomacea canaliculata is the omnivorousness snail based on vegetal bait, and feeding habits are wide, the aquatic cropss such as staple food paddy rice, wild rice stem, duckweed, the sweetish food of preference; Although it is water biological species, of short durationly can leaves water body life, can survive 6 ~ 8 months in moistening earth, once field recovery water layer, activity can take food and cause harm again; In Pomacea canaliculata 1 year 2 ~ 3 generations of breeding, and have obvious generation overlap phenomenon, with young spiral shell or become spiral shell 2 ~ 3cm depths or survive the winter at places such as limit, field, limes marginis under aquatic crops base portion or paddy field soil table; Pieces of an egg often produce the surface of planting bar or drying objects at dried up face 10 ~ more than 15cm, as paddy rice, wild rice stem, barnacle furrow bank, ridge, weeds etc.; 1 female spiral shell can be produced and only hatch young spiral shell 32.5 more than ten thousand for 1 year altogether, the feeding habits of the extremely strong Pomacea canaliculata of fertility are very assorted, almost take food any biology in environment, sometimes also attack other snail and spiral shell ovum, but its Major Foods or higher plant and some dryland vegetables etc.
Along with Pomacea canaliculata is in the increase of China's invasion time, add warming of global climate, the continuous enhancing of Pomacea canaliculata annidation and artificial introducing, its harm area will gradually to the northern area of China migration and expansion; Pomacea canaliculata is suitable for living harm at China's wide geographic area, and harm district area accounts for about 60%, constitutes a serious threat to the Rice Production of China and ecological safety; Simultaneously, in recent years, due to the change of agricultural planting structure, the kind that Pomacea canaliculata harms the crops is also in increase, except hazard rice, Pomacea canaliculata also encroach on be in dark and damp habitat thatch reality, water caltrop, sweet potato, arrowhead, Chinese milk vetch and aquatic vegetable, in recent years, Pomacea canaliculata is widespread harm especially, causes and pays close attention to widely
At present, Pomacea canaliculata control has various ways, as rice field finishing in winter, science Guan Shui, Artificially taking away the egg cases, food spiral shell etc. of tending ducks, but because restricting by factors such as the acquisition of the stability of effect, the selection of action opportunity and material and costs, mostly should not use on a large scale.Pesticide control is one of measure of Pomacea canaliculata control, and the medicament used is chemical agent substantially, except environment being caused to pollution to a certain degree, also there is the problems such as price is higher; The chemicals being sprayed at vegetable surface can because affecting the health of eater, and its residue easily endangers ecotope, and the Pomacea canaliculata in some areas creates resistance to chemical pesticide, makes difficulty of prevention and cure and added cost.
Medicinal eurysome golden thread leech is a kind of aquatic mollusk, also can be movable in the region of offshore humidity.On taxonomy, it belongs to Annelida, Hirudinea, Gnathobdellida, Hirudinidae, gold thread leech genus.Its body is slightly in spindle, and belly is comparatively smooth, and overall figure is more flat, and health is soft, can arbitrarily stretch.Also with food-intake number and change, form whole body by multiple body segment, each body segment exist again multiple body ring.Become the general body length of leech at 6 centimetres ~ 20 cm, wherein the individual larger body length when full body extension can reach 30 cm, body is wide, and generally between 1.3 centimetres ~ 2 centimetres, the back of leech body presents dirty-green usually, has 5 longitudinal grins be made up of yellow and black two kinds of color speckles; The regularity of distribution is one, the center at back, each two of both sides; Respectively there is a yellow longitudinal grin this external leech body side surface lower end.Leech body belly is generally faint yellow, and centre is mingled with many dirty-green irregular blotches, leech body front-end and back-end be respectively anterior sucker and posterior sucker, have absorption and motor function.Eurysome golden thread leech is animal feeding habits, does not suck blood, in water body with the molluscan juice such as spiral shell, freshwater mussel, a species of small clam living in fresh water for food.
In the prevention and control of Pomacea canaliculata, the development and utilization of killing spiral shell plant resources is the pith that biology kills in spiral shell work; Up to now, nearly 1500 Plants are used to ovicidal trait test, and have found that there is many plants and have ovicidal trait; But this is the sub-fraction of Pomacea canaliculata prevention and control, the research space of animal and animal parabiosis prevention and control mechanism also has very large.
Summary of the invention
The object of this invention is to provide a kind of medicinal eurysome golden thread leech to the preventing control method of Pomacea canaliculata.
This medicinal eurysome golden thread leech is to the preventing control method of Pomacea canaliculata:
Adopt medicinal eurysome golden thread leech as object, utilize its happiness draw river snail, freshwater mussel, the molluscan juice of Corbicula fluminea be food life habit, carried out spiral shell leech altogether support catch and kill test, eurysome golden thread leech body weight gain situation experiment and eurysome golden thread leech to Pomacea canaliculata male and female individuality predation contrast difference test;
Result of study draws: eurysome golden thread leech is to the ingestion rate of Pomacea canaliculata, and different with the difference of Pomacea canaliculata Individual Size, it preys on little spiral shell better effects if; Eurysome golden thread leech is in 15 days, and body weight change maximum is the 0.64g in the little spiral shell situation of predation, and minimum of a value is the-0.257g in middle spiral shell situation, and leech body weight change changes relevant with predatory number and self figure simultaneously;
The difference control experiment of eurysome golden thread leech to the predation of Pomacea canaliculata male and female individuality shows: eurysome golden thread leech predatory number difference fluctuates between 0 ~ 0.4, shows the other difference of the predation nonexistence of eurysome golden thread leech to Pomacea canaliculata; Eurysome golden thread leech, sucking in Pomacea canaliculata process, is comparatively vulnerable to external interference, and there is the phenomenon that two eurysome golden thread leech prey on same Pomacea canaliculata simultaneously; More in Pomacea canaliculata quantity, density is larger, when being namely greater than 3: 1, Pomacea canaliculata can be there is and sting drinking water leech postmortem phenomena, but simultaneously, in such circumstances, the accumulative predatory number of medicinal eurysome golden thread leech to Pomacea canaliculata is maximum, shows that medicinal eurysome golden thread leech exists certain control effect to Pomacea canaliculata thus.
Medicinal eurysome golden thread leech, to the preventing control method of Pomacea canaliculata, is specifically made up of following steps:
1.1 materials:
1.1.1 eurysome golden thread leech
Eurysome golden thread leech is bought in propagating field artificially, is about 1 year age of leech, individual body length between 6 centimetres ~ 13 centimetres, weight 5 grams ~ 12 grams, leech body, without scar, is the individuality of health;
1.1.2 Pomacea canaliculata
Testing spiral shell used picks up from paddy field, and spiral shell is 4 monthly ages more than, individual large, and spiral shell shell is intact, be the individuality of health;
1.1.3 instrument and equipment comprises:
The plastic basin of diameter 16cm, tweezers, filter paper, electronic balance, aquarium, Plastic Drum, glass jar;
1.2 experimental techniques:
1.2.1 the raising of eurysome golden thread leech:
In the glass jar of 100cm*50cm*50cm, load the paddy field mud of 5 cm thicks, dig the hole of a wide 30cm of long 50cm betwixt, fill water, enter applicable water plants in surrounding kind, leech is thrown in wherein, and put into shell, river snail, Pomacea canaliculata as feed;
For keeping water quality pure and fresh, within two days, changing a water, choosing and sucked spiral shell shell, and again throw in enough feeds, ensure the clean of overall breeding environment and food source sufficient;
1.2.2 the raising of Pomacea canaliculata:
One-tenth spiral shell is placed in 4 aquariums, and every case is put 40 ~ 50 and is become spiral shell, and breeding density is 30 ~ 35/square metre, add running water exposure in sunshine and leave standstill the filtered water that 48h obtains, the depth of water 30 ~ 35cm, and add appropriate feed, this tests feed used is romaine lettuce, the various leafy vegetable of water spinach;
For keeping water quality pure and fresh, changing a water every day, removing excreta in water, enough feeds of sooner or later throwing something and feeding every day;
1.2.3 spiral shell leech altogether support leech the predation of Pomacea canaliculata is tested:
Select the eurysome golden thread leech of weight between 5.5g ~ 7.5g and Pomacea canaliculata to support in diameter to be altogether in the plastics circle basin of 16cm, to arrange four kinds of process, be respectively:
(1) spread 2cm rice field mud at the bottom of basin, mud face depth of water 1cm, place an eurysome golden thread leech and a Pomacea canaliculata in basin, mud 1v1;
(2) pour the dark filtered water of 2cm in basin into, place an eurysome golden thread leech and a Pomacea canaliculata in basin, water 1v1;
(3) pour the dark filtered water of 2cm in basin into, place an eurysome golden thread leech and five Pomacea canaliculatas in basin, water 1v5;
(4) three eurysome golden thread leech and ten Pomacea canaliculatas are placed in basin, water 3v10;
Each process three repetition, every day entry manage everywhere in eurysome golden thread leech predation situation, altogether test seven days,
Support in process altogether and be found Pomacea canaliculata or eurysome golden thread leech death all can fill into, keep the spiral shell leech constant rate in each contrast, duration of test indoor temperature is 25 ± 2 DEG C, and water used is filtered water;
1.2.4 all ages and classes Pomacea canaliculata and eurysome golden thread leech altogether support leech body weight change test:
Pomacea canaliculata is divided into A, B, C Three Estate by Individual Size: A:0.7g-5.0g, B:5.0g-8.5g, more than C:8.5g-, each grade Pomacea canaliculata Stochastic choice three is only supported in the round basin of diameter 16cm, depth of water 2cm with an eurysome golden thread leech altogether, each process four repetition, observe every day and record eurysome golden thread leech predation situation, once weigh and record after seven days, after 15 days, again carry out weighing record; After first leech surface moisture being drawn with filter paper before weighing, then weigh with electronic balance:
Support in process altogether and be found Pomacea canaliculata or eurysome golden thread leech death all can fill into, keep the spiral shell leech constant rate in each contrast, duration of test indoor temperature is 25 ± 2 DEG C, and water used is filtered water;
1.2.5 eurysome golden thread leech is to the difference test of Pomacea canaliculata male and female individuality predation:
By male and female Pomacea canaliculata separately, and select two ripe male and female and an eurysome golden thread leech to support altogether in the round basin of diameter 16cm water, dark 2cm respectively, five repetitions are set, and every day observed and recorded eurysome golden thread leech to the predation situation of different sexes Pomacea canaliculata, duration of test indoor temperature is 25 ± 2 DEG C, and water used is filtered water;
1.2.6 Pomacea canaliculata standard of perfection anyway:
With reference to Santos method, take out the dead spiral shell of suspection in each process, put in a suitable place to breed respectively and observe in dechlorination water, what can not open operculum activity in 24h is death;
2 interpretations of result:
2.1 spiral shell leech support eurysome golden thread leech altogether to the predation situation of Pomacea canaliculata:
Figure 1 shows that spiral shell leech to support in situation eurysome golden thread leech altogether to the predation result of Pomacea canaliculata, from figure, 1v1 in mud, 1v1 in water, 1v5, under 3v10 these four process, the highest accumulative average predatory number is 17.01 in water in 3v10 process, minimum average predatory number is 5 in mud in the process of 1v1 spiral shell, it is 6 that the 1v1 process of supporting altogether in water adds up predatory number, show thus, eurysome golden thread leech is when Pomacea canaliculata density is larger, ingestion rate is relatively high, if there is earth environment, Pomacea canaliculata can slip in earth hides eurysome golden thread leech predation, thus increase the predation difficulty of eurysome golden thread leech,
Supporting in process altogether, eurysome golden thread leech is to the predation time of Pomacea canaliculata, different with the difference of Pomacea canaliculata Individual Size, absorb the less individual time shorter, the larger individual time is longer, and to the Pomacea canaliculata of 5.0g ~ 8.5g, leech takes food once in every 1.5 hours, to more than 8.5g Pomacea canaliculata, leech takes food once in every 3 hours; According to Germicidal efficacy, eurysome golden thread leech is being sucked in Pomacea canaliculata process, is easily subject to external interference thus stops predation; There is the phenomenon that two eurysome golden thread leech prey on same Pomacea canaliculata simultaneously simultaneously, under 1v5 and 3v10 processes, there are the eurysome golden thread leech phenomena of mortality, and body has wound, doubtfully stung food by Pomacea canaliculata; 2.2 eurysome golden thread leech 15 daily weight growth pattern experiments:
The body weight gain of eurysome golden thread leech, closely related with the quantity of its predation Pomacea canaliculata, support in process altogether and find that Pomacea canaliculata or eurysome golden thread leech death all can fill into, keep spiral shell leech constant rate in each contrast; Experimental result is shown in Fig. 2, eurysome golden thread leech in 15 days, body weight change maximum be the 0.64g in the little spiral shell situation of predation, minimum of a value is the-0.257g in predation in spiral shell situation; Eurysome golden thread leech figure and body weight are also relevant, and when figure becomes full strong state, body weight is heavier, and when figure becomes shrivelled softness, body weight is lighter; Result shows: the ingestion rate of Individual Size to eurysome golden thread leech of Pomacea canaliculata there are differences, and individual large predation is more difficult, and individual little predation is comparatively held;
2.3 eurysome golden thread leech are to the difference control experiment of Pomacea canaliculata male and female individuality predation:
Fig. 3 is the predation situation map of eurysome golden thread leech to male and female Pomacea canaliculata, shown by the experiment of 15 days, contrast each process eurysome golden thread leech predation Pomacea canaliculata quantity, its accumulated quantity preying on female spiral shell is 12, preying on male spiral shell accumulated quantity is 7, to female spiral shell predatory number apparently higher than male spiral shell.
The beneficial effect of the invention:
Employing material is aquatic mollusk, has the advantage that chemicals is incomparable: abundance, with low cost, be zero to the pollution of environment.
Invention has been the screening test of molluscacide: there is certain prevention and control mechanism to Pomacea canaliculata in medicinal eurysome golden thread leech; The predation time of eurysome golden thread leech to Pomacea canaliculata is different with the difference of Pomacea canaliculata Individual Size, and the individual less spiral shell efficiency of predation is faster; Predation can be stopped by external interference in sucking process.
Application eurysome golden thread leech carries out the research eliminating harmful spiral shell, the road of China's Pomacea canaliculata prevention and control can not only be widened, and effectively alleviate the pollution of chemical molluscacide to environment, moreover, medicinal expanded letter gold thread has great medical value, have broad application prospects as utilizing abundant plant resources to provide basic data for Pomacea canaliculata study on prevention further, simultaneously also for bionical pharmaceutical synthesis provides material.
(1) the medicinal eurysome golden thread leech of material is the aquatic mollusk of South China, obtains easily.
(2) processing method is easy and simple to handle, without the need to the program of complexity, is convenient to operate in field.
(3) be conducive to controlling invasion animal Pomacea canaliculata, and open new approach for the recycling of plant, research that is ecological for invasion and biotic intrusion control technology has great reference value.
(4) labour intensity is lower, has wide range of applications, and operating efficiency is higher.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further illustrated.
Pomacea canaliculata prey situation under Fig. 1 different disposal.
Fig. 2 spiral shell leech supports eurysome golden thread leech 15 daily weight variation diagram altogether.
Fig. 3 eurysome golden thread leech is to the predation situation of different sexes Pomacea canaliculata.
Embodiment
Embodiment:
This medicinal eurysome golden thread leech is to the preventing control method of Pomacea canaliculata:
Adopt medicinal eurysome golden thread leech as object, utilize its happiness draw river snail, freshwater mussel, the molluscan juice of Corbicula fluminea be food life habit, carried out spiral shell leech altogether support catch and kill test, eurysome golden thread leech body weight gain situation experiment and eurysome golden thread leech to Pomacea canaliculata male and female individuality predation contrast difference test;
Result of study draws: eurysome golden thread leech is to the ingestion rate of Pomacea canaliculata, and different with the difference of Pomacea canaliculata Individual Size, it preys on little spiral shell better effects if; Eurysome golden thread leech is in 15 days, and body weight change maximum is the 0.64g in the little spiral shell situation of predation, and minimum of a value is the-0.257g in middle spiral shell situation, and leech body weight change changes relevant with predatory number and self figure simultaneously;
The difference control experiment of eurysome golden thread leech to the predation of Pomacea canaliculata male and female individuality shows: eurysome golden thread leech predatory number difference fluctuates between 0 ~ 0.4, shows the other difference of the predation nonexistence of eurysome golden thread leech to Pomacea canaliculata; Eurysome golden thread leech, sucking in Pomacea canaliculata process, is comparatively vulnerable to external interference, and there is the phenomenon that two eurysome golden thread leech prey on same Pomacea canaliculata simultaneously; More in Pomacea canaliculata quantity, density is larger, when being namely greater than 3: 1, Pomacea canaliculata can be there is and sting drinking water leech postmortem phenomena, but simultaneously, in such circumstances, the accumulative predatory number of medicinal eurysome golden thread leech to Pomacea canaliculata is maximum, shows that medicinal eurysome golden thread leech exists certain control effect to Pomacea canaliculata thus.
Medicinal eurysome golden thread leech, to the preventing control method of Pomacea canaliculata, is specifically made up of following steps:
1.1 materials:
1.1.1 eurysome golden thread leech
Eurysome golden thread leech is bought in propagating field artificially, is about 1 year age of leech, individual body length between 6 centimetres ~ 13 centimetres, weight 5 grams ~ 12 grams, leech body, without scar, is the individuality of health;
1.1.2 Pomacea canaliculata
Testing spiral shell used picks up from paddy field, and spiral shell is 4 monthly ages more than, individual large, and spiral shell shell is intact, be the individuality of health;
1.1.3 instrument and equipment comprises:
The plastic basin of diameter 16cm, tweezers, filter paper, electronic balance, aquarium, Plastic Drum, glass jar;
1.2 experimental techniques:
1.2.1 the raising of eurysome golden thread leech:
In the glass jar of 100cm*50cm*50cm, load the paddy field mud of 5 cm thicks, dig the hole of a wide 30cm of long 50cm betwixt, fill water, enter applicable water plants in surrounding kind, leech is thrown in wherein, and put into shell, river snail, Pomacea canaliculata as feed;
For keeping water quality pure and fresh, within two days, changing a water, choosing and sucked spiral shell shell, and again throw in enough feeds, ensure the clean of overall breeding environment and food source sufficient;
1.2.2 the raising of Pomacea canaliculata:
One-tenth spiral shell is placed in 4 aquariums, and every case is put 40 ~ 50 and is become spiral shell, and breeding density is 30 ~ 35/square metre, add running water exposure in sunshine and leave standstill the filtered water that 48h obtains, the depth of water 30 ~ 35cm, and add appropriate feed, this tests feed used is romaine lettuce, the various leafy vegetable of water spinach;
For keeping water quality pure and fresh, changing a water every day, removing excreta in water, enough feeds of sooner or later throwing something and feeding every day;
1.2.3 spiral shell leech altogether support leech the predation of Pomacea canaliculata is tested:
Select the eurysome golden thread leech of weight between 5.5g ~ 7.5g and Pomacea canaliculata to support in diameter to be altogether in the plastics circle basin of 16cm, to arrange four kinds of process, be respectively:
(1) spread 2cm rice field mud at the bottom of basin, mud face depth of water 1cm, place an eurysome golden thread leech and a Pomacea canaliculata in basin, mud 1v1, exactly in the process of the Pomacea canaliculata had in the mud environment of rice field and a leech;
(2) pour the dark filtered water of 2cm in basin into, place an eurysome golden thread leech and a Pomacea canaliculata in basin, water 1v1; A leech of water and the process of 5 Pomacea canaliculatas entirely;
(3) pour the dark filtered water of 2cm in basin into, place an eurysome golden thread leech and five Pomacea canaliculatas in basin, water 1v5;
(4) three eurysome golden thread leech and ten Pomacea canaliculatas are placed in basin, water 3v10;
Each process three repetition, every day entry manage everywhere in eurysome golden thread leech predation situation, altogether test seven days,
Support in process altogether and be found Pomacea canaliculata or eurysome golden thread leech death all can fill into, keep the spiral shell leech constant rate in each contrast, duration of test indoor temperature is 25 ± 2 DEG C, and water used is filtered water;
1.2.4 all ages and classes Pomacea canaliculata and eurysome golden thread leech altogether support leech body weight change test:
Pomacea canaliculata is divided into A, B, C Three Estate by Individual Size: A:0.7g-5.0g, B:5.0g-8.5g, more than C:8.5g-, each grade Pomacea canaliculata Stochastic choice three is only supported in the round basin of diameter 16cm, depth of water 2cm with an eurysome golden thread leech altogether, each process four repetition, observe every day and record eurysome golden thread leech predation situation, once weigh and record after seven days, after 15 days, again carry out weighing record; After first leech surface moisture being drawn with filter paper before weighing, then weigh with electronic balance;
Support in process altogether and be found Pomacea canaliculata or eurysome golden thread leech death all can fill into, keep the spiral shell leech constant rate in each contrast, duration of test indoor temperature is 25 ± 2 DEG C, and water used is filtered water;
1.2.5 eurysome golden thread leech is to the difference test of Pomacea canaliculata male and female individuality predation:
By male and female Pomacea canaliculata separately, and select two ripe male and female and an eurysome golden thread leech to support altogether in the round basin of diameter 16cm water, dark 2cm respectively, five repetitions are set, and every day observed and recorded eurysome golden thread leech to the predation situation of different sexes Pomacea canaliculata, duration of test indoor temperature is 25 ± 2 DEG C, and water used is filtered water;
1.2.6 Pomacea canaliculata standard of perfection anyway:
With reference to Santos method, take out the dead spiral shell of suspection in each process, put in a suitable place to breed respectively and observe in dechlorination water, what can not open operculum activity in 24h is death;
2 interpretations of result:
2.1 spiral shell leech support eurysome golden thread leech altogether to the predation situation of Pomacea canaliculata:
Figure 1 shows that spiral shell leech to support in situation eurysome golden thread leech altogether to the predation result of Pomacea canaliculata, from figure, 1v1 in mud, 1v1 in water, 1v5, under 3v10 these four process, the highest accumulative average predatory number is 17.01 in water in 3v10 process, minimum average predatory number is 5 in mud in the process of 1v1 spiral shell, it is 6 that the 1v1 process of supporting altogether in water adds up predatory number, show thus, eurysome golden thread leech is when Pomacea canaliculata density is larger, ingestion rate is relatively high, if there is earth environment, Pomacea canaliculata can slip in earth hides eurysome golden thread leech predation, thus increase the predation difficulty of eurysome golden thread leech,
Supporting in process altogether, eurysome golden thread leech is to the predation time of Pomacea canaliculata, different with the difference of Pomacea canaliculata Individual Size, absorb the less individual time shorter, the larger individual time is longer, and to the Pomacea canaliculata of 5.0g ~ 8.5g, leech takes food once in every 1.5 hours, to more than 8.5g Pomacea canaliculata, leech takes food once in every 3 hours; According to Germicidal efficacy, eurysome golden thread leech is being sucked in Pomacea canaliculata process, is easily subject to external interference thus stops predation; There is the phenomenon that two eurysome golden thread leech prey on same Pomacea canaliculata simultaneously simultaneously, under 1v5 and 3v10 processes, there are the eurysome golden thread leech phenomena of mortality, and body has wound, doubtfully stung food by Pomacea canaliculata;
2.2 eurysome golden thread leech 15 daily weight growth pattern experiments:
The body weight gain of eurysome golden thread leech, closely related with the quantity of its predation Pomacea canaliculata, support in process altogether and find that Pomacea canaliculata or eurysome golden thread leech death all can fill into, keep spiral shell leech constant rate in each contrast; Experimental result is shown in Fig. 2, eurysome golden thread leech in 15 days, body weight change maximum be the 0.64g in the little spiral shell situation of predation, minimum of a value is the-0.257g in predation in spiral shell situation; Eurysome golden thread leech figure and body weight are also relevant, and when figure becomes full strong state, body weight is heavier, and when figure becomes shrivelled softness, body weight is lighter; Result shows: the ingestion rate of Individual Size to eurysome golden thread leech of Pomacea canaliculata there are differences, and individual large predation is more difficult, and individual little predation is comparatively held;
2.3 eurysome golden thread leech are to the difference control experiment of Pomacea canaliculata male and female individuality predation:
Fig. 3 is the predation situation map of eurysome golden thread leech to male and female Pomacea canaliculata, shown by the experiment of 15 days, contrast each process eurysome golden thread leech predation Pomacea canaliculata quantity, its accumulated quantity preying on female spiral shell is 12, preying on male spiral shell accumulated quantity is 7, to female spiral shell predatory number apparently higher than male spiral shell.

Claims (2)

1. medicinal eurysome golden thread leech is to a preventing control method for Pomacea canaliculata, it is characterized in that:
Adopt medicinal eurysome golden thread leech as object, utilize its happiness draw river snail, freshwater mussel, the molluscan juice of Corbicula fluminea be food life habit, carried out spiral shell leech altogether support catch and kill test, eurysome golden thread leech body weight gain situation experiment and eurysome golden thread leech to Pomacea canaliculata male and female individuality predation contrast difference test;
Result of study draws: eurysome golden thread leech is to the ingestion rate of Pomacea canaliculata, and different with the difference of Pomacea canaliculata Individual Size, it preys on little spiral shell better effects if; Eurysome golden thread leech is in 15 days, and body weight change maximum is the 0.64g in the little spiral shell situation of predation, and minimum of a value is the-0.257g in middle spiral shell situation, and leech body weight change changes relevant with predatory number and self figure simultaneously;
The difference control experiment of eurysome golden thread leech to the predation of Pomacea canaliculata male and female individuality shows: eurysome golden thread leech predatory number difference fluctuates between 0 ~ 0.4, shows the other difference of the predation nonexistence of eurysome golden thread leech to Pomacea canaliculata; Eurysome golden thread leech, sucking in Pomacea canaliculata process, is comparatively vulnerable to external interference, and there is the phenomenon that two eurysome golden thread leech prey on same Pomacea canaliculata simultaneously; More in Pomacea canaliculata quantity, density is larger, when being namely greater than 3: 1, Pomacea canaliculata can be there is and sting drinking water leech postmortem phenomena, but simultaneously, in such circumstances, the accumulative predatory number of medicinal eurysome golden thread leech to Pomacea canaliculata is maximum, shows that medicinal eurysome golden thread leech exists certain control effect to Pomacea canaliculata thus.
2. a kind of medicinal eurysome golden thread leech according to claim 1 is to the preventing control method of Pomacea canaliculata, it is characterized in that: be specifically made up of following steps:
1.1 materials:
1.1.1 eurysome golden thread leech
Eurysome golden thread leech is bought in propagating field artificially, is about 1 year age of leech, individual body length between 6 centimetres ~ 13 centimetres, weight 5 grams ~ 12 grams, leech body, without scar, is the individuality of health;
1.1.2 Pomacea canaliculata
Testing spiral shell used picks up from paddy field, and spiral shell is 4 monthly ages more than, individual large, and spiral shell shell is intact, be the individuality of health;
1.1.3 instrument and equipment comprises:
The plastic basin of diameter 16cm, tweezers, filter paper, electronic balance, aquarium, Plastic Drum, glass jar;
1.2 experimental techniques:
1.2.1 the raising of eurysome golden thread leech:
In the glass jar of 100cm*50cm*50cm, load the paddy field mud of 5 cm thicks, dig the hole of a wide 30cm of long 50cm betwixt, fill water, enter applicable water plants in surrounding kind, leech is thrown in wherein, and put into shell, river snail, Pomacea canaliculata as feed;
For keeping water quality pure and fresh, within two days, changing a water, choosing and sucked spiral shell shell, and again throw in enough feeds, ensure the clean of overall breeding environment and food source sufficient;
1.2.2 the raising of Pomacea canaliculata:
One-tenth spiral shell is placed in 4 aquariums, and every case is put 40 ~ 50 and is become spiral shell, and breeding density is 30 ~ 35/square metre, add running water exposure in sunshine and leave standstill the filtered water that 48h obtains, the depth of water 30 ~ 35cm, and add appropriate feed, this tests feed used is romaine lettuce, the various leafy vegetable of water spinach;
For keeping water quality pure and fresh, changing a water every day, removing excreta in water, enough feeds of sooner or later throwing something and feeding every day;
1.2.3 spiral shell leech altogether support leech the predation of Pomacea canaliculata is tested:
Select the eurysome golden thread leech of weight between 5.5g ~ 7.5g and Pomacea canaliculata to support in diameter to be altogether in the plastics circle basin of 16cm, to arrange four kinds of process, be respectively:
(1) spread 2cm rice field mud at the bottom of basin, mud face depth of water 1cm, place an eurysome golden thread leech and a Pomacea canaliculata in basin, mud 1v1;
(2) pour the dark filtered water of 2cm in basin into, place an eurysome golden thread leech and a Pomacea canaliculata in basin, water 1v1;
(3) pour the dark filtered water of 2cm in basin into, place an eurysome golden thread leech and five Pomacea canaliculatas in basin, water 1v5;
(4) three eurysome golden thread leech and ten Pomacea canaliculatas are placed in basin, water 3v10;
Each process three repetition, every day entry manage everywhere in eurysome golden thread leech predation situation, altogether test seven days,
Support in process altogether and be found Pomacea canaliculata or eurysome golden thread leech death all can fill into, keep the spiral shell leech constant rate in each contrast, duration of test indoor temperature is 25 ± 2 DEG C, and water used is filtered water;
1.2.4 all ages and classes Pomacea canaliculata and eurysome golden thread leech altogether support leech body weight change test:
Pomacea canaliculata is divided into A, B, C Three Estate by Individual Size: A:0.7g-5.0g, B:5.0g-8.5g, more than C:8.5g-, each grade Pomacea canaliculata Stochastic choice three is only supported in the round basin of diameter 16cm, depth of water 2cm with an eurysome golden thread leech altogether, each process four repetition, observe every day and record eurysome golden thread leech predation situation, once weigh and record after seven days, after 15 days, again carry out weighing record; After first leech surface moisture being drawn with filter paper before weighing, then weigh with electronic balance;
Support in process altogether and be found Pomacea canaliculata or eurysome golden thread leech death all can fill into, keep the spiral shell leech constant rate in each contrast, duration of test indoor temperature is 25 ± 2 DEG C, and water used is filtered water;
1.2.5 eurysome golden thread leech is to the difference test of Pomacea canaliculata male and female individuality predation:
By male and female Pomacea canaliculata separately, and select two ripe male and female and an eurysome golden thread leech to support altogether in the round basin of diameter 16cm water, dark 2cm respectively, five repetitions are set, and every day observed and recorded eurysome golden thread leech to the predation situation of different sexes Pomacea canaliculata, duration of test indoor temperature is 25 ± 2 DEG C, and water used is filtered water;
1.2.6 Pomacea canaliculata standard of perfection anyway:
With reference to Santos method, take out the dead spiral shell of suspection in each process, put in a suitable place to breed respectively and observe in dechlorination water, what can not open operculum activity in 24h is death;
2 interpretations of result:
2.1 spiral shell leech support eurysome golden thread leech altogether to the predation situation of Pomacea canaliculata:
Figure 1 shows that spiral shell leech to support in situation eurysome golden thread leech altogether to the predation result of Pomacea canaliculata, from figure, 1v1 in mud, 1v1 in water, 1v5, under 3v10 these four process, the highest accumulative average predatory number is 17.01 in water in 3v10 process, minimum average predatory number is 5 in mud in the process of 1v1 spiral shell, it is 6 that the 1v1 process of supporting altogether in water adds up predatory number, show thus, eurysome golden thread leech is when Pomacea canaliculata density is larger, ingestion rate is relatively high, if there is earth environment, Pomacea canaliculata can slip in earth hides eurysome golden thread leech predation, thus increase the predation difficulty of eurysome golden thread leech,
Supporting in process altogether, eurysome golden thread leech is to the predation time of Pomacea canaliculata, different with the difference of Pomacea canaliculata Individual Size, absorb the less individual time shorter, the larger individual time is longer, and to the Pomacea canaliculata of 5.0g ~ 8.5g, leech takes food once in every 1.5 hours, to more than 8.5g Pomacea canaliculata, leech takes food once in every 3 hours; According to Germicidal efficacy, eurysome golden thread leech is being sucked in Pomacea canaliculata process, is easily subject to external interference thus stops predation; There is the phenomenon that two eurysome golden thread leech prey on same Pomacea canaliculata simultaneously simultaneously, under 1v5 and 3v10 processes, there are the eurysome golden thread leech phenomena of mortality, and body has wound, doubtfully stung food by Pomacea canaliculata;
2.2 eurysome golden thread leech 15 daily weight growth pattern experiments:
The body weight gain of eurysome golden thread leech, closely related with the quantity of its predation Pomacea canaliculata, support in process altogether and find that Pomacea canaliculata or eurysome golden thread leech death all can fill into, keep spiral shell leech constant rate in each contrast; Experimental result is shown in Fig. 2, eurysome golden thread leech in 15 days, body weight change maximum be the 0.64g in the little spiral shell situation of predation, minimum of a value is the-0.257g in predation in spiral shell situation; Eurysome golden thread leech figure and body weight are also relevant, and when figure becomes full strong state, body weight is heavier, and when figure becomes shrivelled softness, body weight is lighter; Result shows: the ingestion rate of Individual Size to eurysome golden thread leech of Pomacea canaliculata there are differences, and individual large predation is more difficult, and individual little predation is comparatively held;
2.3 eurysome golden thread leech are to the difference control experiment of Pomacea canaliculata male and female individuality predation:
Fig. 3 is the predation situation map of eurysome golden thread leech to male and female Pomacea canaliculata, shown by the experiment of 15 days, contrast each process eurysome golden thread leech predation Pomacea canaliculata quantity, its accumulated quantity preying on female spiral shell is 12, preying on male spiral shell accumulated quantity is 7, to female spiral shell predatory number apparently higher than male spiral shell.
CN201510422298.XA 2015-07-20 2015-07-20 Method for controlling pomacea canaliculata by utilizing medicinal whitmania pigra Pending CN105123624A (en)

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