CN105104312A - Stiff silkworm culture method - Google Patents
Stiff silkworm culture method Download PDFInfo
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- CN105104312A CN105104312A CN201510431732.0A CN201510431732A CN105104312A CN 105104312 A CN105104312 A CN 105104312A CN 201510431732 A CN201510431732 A CN 201510431732A CN 105104312 A CN105104312 A CN 105104312A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
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Abstract
The invention discloses a stiff silkworm culture method. The stiff silkworm culture method comprises the following steps of a. preparing a spore suspension: taking beauveria bassiana spores to prepare the spore suspension; b. infecting: feeding young silkworms; 12-24 hours after the young silkworms wake up from the fourth dormancy, spraying and inoculating the spore suspension obtained in the step a; feeding the young silkworms under the conditions that the temperature is 23-25 DEG C and the humidity is more than 95%; after 4 hours, repeatedly spraying and inoculating for one time; c. feeding the young silkworms which are infected in the step b for 48 hours under the conditions that the temperature is 23-25 DEG C and the humidity is more than 95%; feeding the young silkworms in a natural environment until the domestic silkworms grow up, harvesting and drying the silkworms. The stiff silkworm culture method disclosed by the invention can be used for culturing the stiff silkworms in a large scale and has high and stable yield; the quality of the cultured stiff silkworms is excellent and the industrial application prospect is good.
Description
Technical field
The present invention relates to the cultural method of a kind of stiff silkworm.
Background technology
Stiff silkworm, the young silkworm for Bombycidae insect silkworm BombyxmoriLinnaeus4 ~ 5 ages infects (or artificial infection) white muscardine fungi Beauveriabassiana (Bals.) Vuillant and lethal dry body.Be distributed in the ground such as Jiangsu, Zhejiang, Sichuan, Guangdong.There is breath wind and stop convulsion, wind-expelling pain-stopping, effect of reducing phlegm and resolving masses.Commonly used in autumn zephyrlily phlegm, chronic ulcerated tic, infantile surprised, tetanus, wai in tuyere wind hot headaches, conjunctival congestion, sore throat, rubella itching, hair h mumps.
Existing stiff silkworm preparation method is comparatively extensive, and the time of inoculation can be any time of young silkworm in 4 ~ 5 ages, and postvaccinal epidemic disaster controls also strict, usually raises under natural environment, and cause yielding poorly and instability of stiff silkworm, quality is also without guarantee.
Summary of the invention
Cultivating Problems existing to solve existing stiff silkworm, the invention provides a kind of new stiff silkworm breeding method.
The cultural method of the stiff silkworm of the present invention, comprises the following steps:
A, prepare spore suspension: get beauveria bassiana spore, prepare spore suspension;
B, to infect: raise young silkworm, 12 ~ 24h after the 4th dormancy of young silkworm is revived, spray inoculation step a gained spore suspension, be placed in that temperature is 23 ~ 25 DEG C, humidity is raise under the condition of more than 95%, repeat after 4h to spray inoculation once;
C, continue to raise: the young silkworm of step b after infecting is placed in temperature is 23 ~ 25 DEG C, humidity continues to raise 48h under being greater than the condition of 95%, then raise to silkworm ripe in natural environment, results, dry process, to obtain final product.
Silkworm is ripe: refer to that silkworm does not eat mulberry leaf and cocoons.
In step a, the spore concentration of described suspension is not less than 10
6individual spore/ml.Further preferably, described spore suspension contains 10
6~ 10
9individual spore/ml.
In step a, described white muscardine fungi is beauveria bassiana (Beauveriabassiana (Bals.) Vuill).
In step b, the amount of described inoculation is that every silkworm inoculates 0.1-0.3ml spore suspension at every turn.
Present invention also offers preceding method and prepare the stiff silkworm of part.
The present invention is by specific inoculation time, inoculation method, and the mode of raising under specific temperature and humidity conditions after inoculation, reaches the object improving stiff silkworm seed output and quality.
Stiff silkworm cultural method provided by the invention can the stiff silkworm of large-scale culture, and output is high and stable, and it is good to cultivate the stiff silkworm quality obtained, and prospects for commercial application is good.
The embodiment of form by the following examples, is described in further detail foregoing of the present invention.But this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following embodiment.All technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment
Beauveria bassiana (Beauveriabassiana (Bals.) Vuill), buys from Southwestern University.
The cultural method of the stiff silkworm of embodiment 1
(1) cultural method
1, beauveria bassiana spore suspension preparation
Get beauveria bassiana (Beauveriabassiana), make containing 10
6the suspension of individual/ml spore.
2, infect
Raise young silkworm by the conventional rearing method of silkworm, young silkworm ensures young silkworm healthy development.
After the 4th dormancy of young silkworm is revived, 12h sprays spore suspension, and the fountain height of every silkworm is 0.1-0.3ml, is placed in that temperature is 23 DEG C, humidity is raise under the condition of more than 95%, and again spray after 4h, fountain height is identical.
3, continue to raise
Infect be placed on 23 DEG C, humidity is continue raising 48 hours under the condition of more than 95%, then raises silkworm under natural conditions ripe, results, dry process.
(2) testing result
Output a: silkworm egg (silkworm egg contains 12g silkworm egg) can gather in the crops stiff silkworm 12-15 kilogram/.
Quality: glass rim (glass rim refers to that section is smooth, outer layer white, and there is four, the sericterium ring of bright green centre) all appears in stiff silkworm, and quality is good.
The cultural method of the stiff silkworm of embodiment 2
(1) cultural method
1, beauveria bassiana spore suspension preparation
Get beauveria bassiana (Beauveriabassiana), make containing 10
9the suspension of individual/ml spore.
2, infect
Raise young silkworm by the conventional rearing method of silkworm, ensure young silkworm healthy development.
After the 4th dormancy of young silkworm is revived, 24h sprays spore suspension, and the fountain height of every silkworm is 0.1-0.3ml, is placed in that temperature is 24 DEG C, humidity is raise under the condition of more than 95%, and again spray after 4h, fountain height is identical.
3, continue to raise
Infect be placed on 24 DEG C, humidity is continue raising 48 hours under the condition of more than 95%, then raises silkworm under natural conditions ripe, results, dry process.
(2) testing result
Output a: silkworm egg (silkworm egg contains 12g silkworm egg) can gather in the crops stiff silkworm 12-15 kilogram/.
Quality: glass rim (glass rim refers to that section is smooth, outer layer white, and there is four, the sericterium ring of bright brown or bright black centre) all appears in stiff silkworm, and quality is good.
The cultural method of the stiff silkworm of embodiment 3
(1) cultural method
1, beauveria bassiana spore suspension preparation
Get beauveria bassiana (Beauveriabassiana), make containing 10
8the suspension of individual/ml spore.
2, infect
Raise young silkworm by the conventional rearing method of silkworm, ensure young silkworm healthy development.
After the 4th dormancy of young silkworm is revived, 18h sprays spore suspension, and the fountain height of every silkworm is 0.1-0.3ml, is placed in that temperature is 25 DEG C, humidity is raise under the condition of more than 95%, and again spray after 4h, fountain height is identical.
3, continue to raise
Infect be placed on 25 DEG C, humidity is continue raising 48 hours under the condition of more than 95%, then raises silkworm under natural conditions ripe, results, dry process.
(2) testing result
Output: silkworm egg (silkworm egg contains 12g silkworm egg) can gather in the crops stiff silkworm 12 kilograms/.
Quality: glass rim (glass rim refers to that section is smooth, outer layer white, and there is four, the sericterium ring of bright green centre) all appears in stiff silkworm, and quality is good.
Comparative example 1
(1) cultural method
1, beauveria bassiana spore suspension preparation
Get beauveria bassiana (Beauveriabassiana), make containing 10
9the suspension of individual/ml spore.
2, infect
Raise young silkworm by the conventional rearing method of silkworm, ensure young silkworm healthy development.
After the 4th dormancy of young silkworm is revived, 48h sprays spore suspension, and the fountain height of every silkworm is 0.1-0.3ml, is placed in that temperature is 25 DEG C, humidity is raise under the condition of more than 95%, and again spray after 4h, fountain height is identical.
3, continue to raise
Infect be placed on 25 DEG C, humidity is continue raising 48 hours under the condition of more than 95%, then raises silkworm under natural conditions ripe, results, dry process.
(2) testing result
Output: silkworm egg (silkworm egg contains 12g silkworm egg) can gather in the crops stiff silkworm 8 kilograms/.
Comparative example 2
(1) cultural method
1, beauveria bassiana spore suspension preparation
Get beauveria bassiana (Beauveriabassiana), make containing 10
9the suspension of individual/ml spore.
2, infect
Raise young silkworm by the conventional rearing method of silkworm, ensure young silkworm healthy development.
After the 4th dormancy of young silkworm is revived, 4h sprays spore suspension, and the fountain height of every silkworm is 0.1-0.3ml, is placed in that temperature is 23 DEG C, humidity is raise under the condition of more than 95%, and again spray after 4h, fountain height is identical.
3, continue to raise
Infect be placed on 23 DEG C, humidity is continue raising 48 hours under the condition of more than 95%, then raises silkworm under natural conditions ripe, results, dry process.
(2) testing result
Output a: silkworm egg (silkworm egg contains 12g silkworm egg) can gather in the crops stiff silkworm 12-15 kilogram/.
Quality: have hollow phenomenon, quality is not good.
Comparative example 3
(1) cultural method
1, beauveria bassiana spore suspension preparation
Get beauveria bassiana (Beauveriabassiana), make containing 10
9the suspension of individual/ml spore.
2, infect
Raise young silkworm by the conventional rearing method of silkworm, ensure young silkworm healthy development.
After the 4th dormancy of young silkworm is revived, 24h sprays spore suspension, and the fountain height of every silkworm is 0.1-0.3ml, is placed in that temperature is 22 DEG C, humidity is raise under the condition of more than 95%, and again spray after 4h, fountain height is identical.
3, continue to raise
Infect be placed on 22 DEG C, humidity is continue raising 48 hours under the condition of more than 95%, then raises silkworm under natural conditions ripe, results, dry process.
(2) testing result
Output: silkworm egg (silkworm egg contains 12g silkworm egg) can gather in the crops stiff silkworm 10 kilograms/.
Comparative example 4
(1) cultural method
1, beauveria bassiana spore suspension preparation
Get beauveria bassiana (Beauveriabassiana), make containing 10
9the suspension of individual/ml spore.
2, infect
Raise young silkworm by the conventional rearing method of silkworm, ensure young silkworm healthy development.
After the 4th dormancy of young silkworm is revived, 24h sprays spore suspension, and the fountain height of every silkworm is 0.1-0.3ml, is placed in that temperature is 26 DEG C, humidity is raise under the condition of more than 95%, and again spray after 4h, fountain height is identical.
3, continue to raise
Infect be placed on 26 DEG C, humidity is continue raising 48 hours under the condition of more than 95%, then raises silkworm under natural conditions ripe, results, dry process.
(2) testing result
Output: silkworm egg (silkworm egg contains 12g silkworm egg) can gather in the crops stiff silkworm 9 kilograms/.
Quality: occur glass rim, but also there are other flacheries, and quality is not good.
Stiff silkworm cultural method provided by the invention can the stiff silkworm of large-scale culture, and output is high and stable, and it is good to cultivate the stiff silkworm quality obtained, and adopts other inoculation methods or cultural method, be then difficult to the seed output and quality simultaneously ensureing stiff silkworm.
Claims (6)
1. a cultural method for stiff silkworm, is characterized in that: comprise the following steps:
A, prepare spore suspension: get beauveria bassiana spore, prepare spore suspension;
B, to infect: raise young silkworm, 12 ~ 24h after the 4th dormancy of young silkworm is revived, spray inoculation step a gained spore suspension, be placed in that temperature is 23 ~ 25 DEG C, humidity is raise under the condition of more than 95%, repeat after 4h to spray inoculation once;
C, continue to raise: the young silkworm after being infected by step b is placed in that temperature is 23 ~ 25 DEG C, humidity continues to raise 48h under being greater than the condition of 95%, then raises to silkworm ripe in natural environment, results, and dry process, to obtain final product.
2. cultural method according to claim 1, is characterized in that: in step a, the spore concentration of described suspension is not less than 10
6individual spore/ml.
3. want the cultural method described in 2 according to right, it is characterized in that: described spore suspension contains 10
6~ 10
9individual spore/ml.
4. cultural method according to claim 1, is characterized in that: in step a, and described white muscardine fungi is beauveria bassiana (Beauveriabassiana (Bals.) Vuill).
5. cultural method according to claim 1, is characterized in that: in step b, and the amount of described inoculation is that every silkworm inoculates 0.1-0.3ml spore suspension at every turn.
6. the stiff silkworm of preparing of method described in Claims 1 to 5 any one.
Priority Applications (1)
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|---|---|---|---|
| CN201510431732.0A CN105104312A (en) | 2015-07-21 | 2015-07-21 | Stiff silkworm culture method |
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|---|---|---|---|
| CN201510431732.0A CN105104312A (en) | 2015-07-21 | 2015-07-21 | Stiff silkworm culture method |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106614423A (en) * | 2016-12-30 | 2017-05-10 | 四川省农业科学院蚕业研究所 | Artificial preparation method of white muscardine silkworms |
| CN106719472A (en) * | 2016-12-01 | 2017-05-31 | 四川省农业科学院蚕业研究所 | A kind of production method of the larva of a silkworm with batrytis |
| CN107258705A (en) * | 2017-06-28 | 2017-10-20 | 四川腾弘农业发展有限公司 | A kind of method that scale collects the larva of a silkworm with batrytis |
| CN107494458A (en) * | 2017-08-15 | 2017-12-22 | 广西八园农业科技有限公司 | Method that is a kind of while producing flat board silk and the larva of a silkworm with batrytis |
| CN109221031A (en) * | 2018-10-17 | 2019-01-18 | 成都中医药大学 | A kind of is suitable for the inoculation method of medicinal bombyx batryticatus production |
| CN109221030A (en) * | 2018-10-17 | 2019-01-18 | 成都中医药大学 | A kind of medicinal bombyx batryticatus cultural method of good quality and high output |
| CN109985066A (en) * | 2018-10-17 | 2019-07-09 | 成都岷江源药业股份有限公司 | A kind of processing method of high-quality bombyx batryticatus |
| CN110051697A (en) * | 2018-10-17 | 2019-07-26 | 成都岷江源药业股份有限公司 | A kind of collecting method of high-quality bombyx batryticatus |
| CN110063304A (en) * | 2018-10-17 | 2019-07-30 | 成都岷江源药业股份有限公司 | A kind of is suitable for the environment control method of medicinal bombyx batryticatus production |
| CN110063303B (en) * | 2018-10-17 | 2021-09-24 | 成都岷江源药业股份有限公司 | Silkworm seed composition suitable for producing medicinal stiff silkworm |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102232372A (en) * | 2011-04-28 | 2011-11-09 | 梁文荣 | Equipment and method for supplementary incubation of spring silkworm eggs |
| CN103923841A (en) * | 2014-04-14 | 2014-07-16 | 广东省农业科学院蚕业与农产品加工研究所 | Beauveria bassiana strain having high pathogenicity to silkworms and application thereof |
| CN104145889A (en) * | 2014-08-25 | 2014-11-19 | 广东省农业科学院蚕业与农产品加工研究所 | Improved efficient white muscardine silkworm cultivating method |
| CN104430212A (en) * | 2014-12-17 | 2015-03-25 | 广东省农业科学院蚕业与农产品加工研究所 | Spore suspension for improving yield of white muscardine silkworms and application of spore suspension |
-
2015
- 2015-07-21 CN CN201510431732.0A patent/CN105104312A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102232372A (en) * | 2011-04-28 | 2011-11-09 | 梁文荣 | Equipment and method for supplementary incubation of spring silkworm eggs |
| CN103923841A (en) * | 2014-04-14 | 2014-07-16 | 广东省农业科学院蚕业与农产品加工研究所 | Beauveria bassiana strain having high pathogenicity to silkworms and application thereof |
| CN104145889A (en) * | 2014-08-25 | 2014-11-19 | 广东省农业科学院蚕业与农产品加工研究所 | Improved efficient white muscardine silkworm cultivating method |
| CN104430212A (en) * | 2014-12-17 | 2015-03-25 | 广东省农业科学院蚕业与农产品加工研究所 | Spore suspension for improving yield of white muscardine silkworms and application of spore suspension |
Non-Patent Citations (1)
| Title |
|---|
| 邢东旭等: "一株感染家蚕的高致病性白僵菌的鉴定及在僵蚕生产中的应用", 《蚕业科学》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719472A (en) * | 2016-12-01 | 2017-05-31 | 四川省农业科学院蚕业研究所 | A kind of production method of the larva of a silkworm with batrytis |
| CN106614423A (en) * | 2016-12-30 | 2017-05-10 | 四川省农业科学院蚕业研究所 | Artificial preparation method of white muscardine silkworms |
| CN106614423B (en) * | 2016-12-30 | 2020-08-14 | 四川省农业科学院蚕业研究所 | Artificial preparation method of white muscardine silkworm |
| CN107258705A (en) * | 2017-06-28 | 2017-10-20 | 四川腾弘农业发展有限公司 | A kind of method that scale collects the larva of a silkworm with batrytis |
| CN107258705B (en) * | 2017-06-28 | 2019-09-24 | 四川腾弘农业发展有限公司 | A kind of method that scale collects the larva of a silkworm with batrytis |
| CN107494458A (en) * | 2017-08-15 | 2017-12-22 | 广西八园农业科技有限公司 | Method that is a kind of while producing flat board silk and the larva of a silkworm with batrytis |
| CN109221031A (en) * | 2018-10-17 | 2019-01-18 | 成都中医药大学 | A kind of is suitable for the inoculation method of medicinal bombyx batryticatus production |
| CN109221030A (en) * | 2018-10-17 | 2019-01-18 | 成都中医药大学 | A kind of medicinal bombyx batryticatus cultural method of good quality and high output |
| CN109985066A (en) * | 2018-10-17 | 2019-07-09 | 成都岷江源药业股份有限公司 | A kind of processing method of high-quality bombyx batryticatus |
| CN110051697A (en) * | 2018-10-17 | 2019-07-26 | 成都岷江源药业股份有限公司 | A kind of collecting method of high-quality bombyx batryticatus |
| CN110063304A (en) * | 2018-10-17 | 2019-07-30 | 成都岷江源药业股份有限公司 | A kind of is suitable for the environment control method of medicinal bombyx batryticatus production |
| CN110063303B (en) * | 2018-10-17 | 2021-09-24 | 成都岷江源药业股份有限公司 | Silkworm seed composition suitable for producing medicinal stiff silkworm |
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