CN105087382A - Cell thawing explosion-proof egg - Google Patents
Cell thawing explosion-proof egg Download PDFInfo
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- CN105087382A CN105087382A CN201510622688.1A CN201510622688A CN105087382A CN 105087382 A CN105087382 A CN 105087382A CN 201510622688 A CN201510622688 A CN 201510622688A CN 105087382 A CN105087382 A CN 105087382A
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- eggshell
- explosion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/20—Heating; Cooling
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Abstract
The invention relates to a cell thawing explosion-proof egg which comprises a lower half egg (1) and an upper half egg (2) which can be combined together, wherein the bottom of the lower half egg (1) is a lead bottom (4); air holes (5) and a sampling tube (3) are arranged on the upper half egg (2). The cell thawing explosion-proof egg is simple in structure, and convenient to use, and the problem that a tester can be injured due to explosion of a cell cryopreservation tube during a cell thawing process is solved; moreover, due to the design of the lead bottom, the cell thawing explosion-proof egg can wobble as a tumbler, so that the cell cryopreservation tube can be heated uniformly to ensure the cell thawing effect.
Description
Technical field
The invention belongs to cell recovery device technique field, be specifically related to the explosion-proof egg of a kind of cell recovery.
Background technology
In the experimental implementation relevant to cell, often to carry out freezing and thawing to cell.Cell recovery is working foundation important in Evaluation of Biocompatibility system with cultivating.The principle of cell recovery is fast melt, must by frozen cell fast melt to 37 DEG C under-196 DEG C of liquid nitrogen or other low temperature environments, ice crystal when making extracellular frozen melts rapidly, enter cell when avoiding ice crystal slowly to melt and form recrystallize, cell is caused damage, especially when 0-5 DEG C, cell is more easily destroyed, so frozen cell must be made to spend this temperature the soonest.
The operating process of cell recovery is generally: from liquid nitrogen or other low temperature environments, take out cell cryopreservation tube, directly immerses in 37 DEG C of warm water, and shake makes it melt as early as possible frequently.Cell cryopreservation tube is taken out, uncap more subsequently, with suction pipe sucking-off cell suspension from 37 DEG C of water-baths.In order to make cell cryopreservation tube be heated evenly when cell recovery, reducing ice crystal and occurring, usually in cell recovery process, experimenter needs be placed in 37 DEG C of water-baths with hand-held cell cryopreservation tube and rock this cell cryopreservation tube.But enter due to liquid nitrogen may be had in cell cryopreservation tube, in the process be rapidly heated, liquid nitrogen gasifies rapidly and causes cell cryopreservation tube to blast, although general experimenter can protect eyes with eye-protection glasses, but the hand of other positions of health especially exposing cell cryopreservation tube only has generic latex gloves to protect usually, emgloves can be caused to break at cell cryopreservation tube explosion time, often occur the situation that experimenter's hand is slightly injured.
In order to solve cell cryopreservation tube explosion issues, the scheme usually adopted clamps cell cryopreservation tube with tweezers to rock in water, brings muffler to protect simultaneously, but the method operation comparatively inconvenience, and still there is the possibility that other parts of health come to harm.Just cover the method for water-bath immediately with lid after also having part test personnel employing that cell cryopreservation tube is placed in water-bath, but because generally conventional water-bath does not all have swirling device, if do not shake cell cryopreservation tube, its inequality and affect cell recovery effect of being easily heated, and still cannot avoid the blast that occurred when cell cryopreservation tube being just placed in water-bath.
In order to solve the problem, the present invention is proposed.
Summary of the invention
The object of this invention is to provide the explosion-proof egg of a kind of cell recovery, testing crew to be damaged and cell cryopreservation tube is heated the uneven problem affecting cell recovery effect to solve the blast of in cell recovery process cell cryopreservation tube.
First aspect present invention relates to the explosion-proof egg of a kind of cell recovery, and it comprises second eggshell 1 and first eggshell 2 that can combine; The bottom of second eggshell 1 wherein said is the plumbous end 4, and first eggshell 2 described has pore 5 and sample feeding pipe 3.
In a preferred embodiment of the present invention, be connected by the band 6 that is flexible coupling between second eggshell 1 described with first eggshell 2 described.
In a preferred embodiment of the present invention, can engage or be threaded connection between second eggshell 1 described with first eggshell 2 described.
In a preferred embodiment of the present invention, the quantity of described sample feeding pipe 3 is two.
In a preferred embodiment of the present invention, the length of described sample feeding pipe 3 is 0-3cm.
In a preferred embodiment of the present invention, described pore 5 is positioned at the top of first eggshell 2 described, and the upper end of described pore 5 is reticulated structure.The effect of this pore 5 is the outlets as the gas in the explosion-proof egg of this cell recovery, and described pore 5 is preferably fine mesh structure, spills to prevent the object in the explosion-proof egg of cell recovery.
In a preferred embodiment of the present invention, when second eggshell 1 described and first eggshell 2 described combine, both also have sealing-ring in periphery, junction.
Device of the present invention is an egg type cell recovery device, and its use procedure is as follows: separately second eggshell 1 described and first eggshell 2 described, injects the warm water 8 of 37-39 DEG C, cover first eggshell 2 described in second eggshell 1 described; This device is taken the liquid nitrogen container place to freeze-stored cell, then cell cryopreservation tube 7 is taken out from liquid nitrogen, the explosion-proof egg of cell recovery is put into from the sample feeding pipe 3 first eggshell 2 described, and the power of giving one, the explosion-proof egg of this cell recovery initial, make it naturally rock on testing table as " tumbler ", first eggshell 2 described in opening after 5min takes out described cell cryopreservation tube 7 can carry out next step test.
Second aspect present invention relates to the method for the explosion-proof egg of cell recovery described in first aspect present invention for cell recovery, and it comprises the following steps:
A. separate described second eggshell (1) and first eggshell described (2), in described second eggshell (1), inject the warm water (8) of 37-39 DEG C, cover described first eggshell (2);
B. cell cryopreservation tube (7) is taken out from liquid nitrogen, the explosion-proof egg of this cell recovery is put into from the sample feeding pipe (3) described first eggshell (2), and the thrust of giving one, the explosion-proof egg of this cell recovery initial, make it naturally rock and carry out cell recovery.
Third aspect present invention relates to the purposes of the explosion-proof egg of cell recovery described in first aspect present invention for cell recovery.
In a preferred embodiment of the present invention, described purposes is for preventing cell cryopreservation tube blast in cell recovery process.
The present invention achieves following beneficial effect:
Cell recovery of the present invention explosion-proof egg structure is simple, easy to use, solves the problem that cell cryopreservation tube blast can damage testing crew in cell recovery process; Meanwhile, the design at the plumbous end makes the explosion-proof egg of this cell recovery can rock as " tumbler ", thus cell cryopreservation tube is heated evenly, and ensures cell recovery effect.
Accompanying drawing explanation
Fig. 1 is the explosion-proof egg overall schematic of cell recovery of the present invention.
Fig. 2 is that rear schematic diagram opened by the explosion-proof egg of cell recovery of the present invention.
Wherein each Reference numeral has following implication:
Second eggshell of 1-; First eggshell of 2-; 3-sample feeding pipe; The 4-plumbous end; 5-pore; 6-is flexible coupling band; 7-cell cryopreservation tube; 8-warm water.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is explained in further detail, when should be understood that embodiment is only illustrative, and not restrictive.
Embodiment 1
The explosion-proof egg of cell recovery as depicted in figs. 1 and 2, it comprises second eggshell 1 and first eggshell 2 that can combine; The bottom of second eggshell 1 wherein said is the plumbous end 4, first eggshell 2 described has 1 pore 5 and 2 sample feeding pipes 3.Be connected by the band 6 that is flexible coupling between second eggshell 1 described with first eggshell 2 described, described pore 5 is positioned at the top of first eggshell 2 described.Wherein said sample feeding pipe 3 has certain length, its objective is to prevent cell cryopreservation tube 7 explosion time, and water or other materials are from the upper ejection of this sample feeding pipe 3.
The use procedure of this device is as follows: separately second eggshell 1 described and first eggshell 2 described, injects the warm water 8 of 37-39 DEG C, cover first eggshell 2 described in second eggshell 1 described; This device is taken the liquid nitrogen container place to freeze-stored cell, then cell cryopreservation tube 7 is taken out from liquid nitrogen, the explosion-proof egg of cell recovery is put into from the sample feeding pipe 3 first eggshell 2 described, and the power of giving one, the explosion-proof egg of this cell recovery initial, make it naturally rock on testing table as " tumbler ", first eggshell 2 described in opening after 5min takes out described cell cryopreservation tube 7 can carry out next step test.
Cell recovery of the present invention explosion-proof egg structure is simple, easy to use, has very strong practicality in cell recovery field.
Claims (10)
1. the explosion-proof egg of cell recovery, is characterized in that, it comprises second eggshell (1) and first eggshell (2) that can combine; The bottom of wherein said second eggshell (1) is the plumbous end (4), described first eggshell (2) has pore (5) and sample feeding pipe (3).
2. the explosion-proof egg of cell recovery according to claim 1, is characterized in that, is with (6) to be connected between described second eggshell (1) with described first eggshell (2) by being flexible coupling.
3. the explosion-proof egg of cell recovery according to claim 1, is characterized in that, can engage or be threaded connection between described second eggshell (1) with described first eggshell (2).
4. the explosion-proof egg of cell recovery according to claim 1, is characterized in that, the quantity of described sample feeding pipe (3) is two.
5. the explosion-proof egg of the cell recovery according to claim 1 or 4, is characterized in that, the length of described sample feeding pipe (3) is 0-3cm.
6. the explosion-proof egg of cell recovery according to claim 1, is characterized in that, described pore (5) is positioned at the top of described first eggshell (2), and the upper end of described pore (5) is reticulated structure.
7. the explosion-proof egg of the cell recovery according to claim 1 or 3, is characterized in that, when described second eggshell (1) and first eggshell described (2) combine, both also have sealing-ring in periphery, junction.
8. the explosion-proof egg of cell recovery according to claim 1 is used for the method for cell recovery, and it is characterized in that, it comprises the following steps:
A. separate described second eggshell (1) and first eggshell described (2), in described second eggshell (1), inject the warm water (8) of 37-39 DEG C, cover described first eggshell (2);
B. cell cryopreservation tube (7) is taken out from liquid nitrogen, the explosion-proof egg of this cell recovery is put into from the sample feeding pipe (3) described first eggshell (2), and the thrust of giving one, the explosion-proof egg of this cell recovery initial, make it naturally rock and carry out cell recovery.
9. the explosion-proof egg of cell recovery according to claim 1 is used for the purposes of cell recovery.
10. purposes according to claim 9, is characterized in that, it is for preventing cell cryopreservation tube blast in cell recovery process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510622688.1A CN105087382B (en) | 2015-09-25 | 2015-09-25 | A kind of explosion-proof egg of cell recovery |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510622688.1A CN105087382B (en) | 2015-09-25 | 2015-09-25 | A kind of explosion-proof egg of cell recovery |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105087382A true CN105087382A (en) | 2015-11-25 |
| CN105087382B CN105087382B (en) | 2017-07-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510622688.1A Active CN105087382B (en) | 2015-09-25 | 2015-09-25 | A kind of explosion-proof egg of cell recovery |
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| CN (1) | CN105087382B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108277152A (en) * | 2018-04-04 | 2018-07-13 | 徐俊 | The sterile liquid changing device of cell recovery and carry out the method that cell recovery changes liquid using the device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202401064U (en) * | 2011-12-29 | 2012-08-29 | 郑州伊美诺生物技术有限公司 | Suspension resuscitation device applicable to quick resuscitation of cells |
| CN104061829A (en) * | 2014-06-03 | 2014-09-24 | 北京空间飞行器总体设计部 | Portable and deformable energy adsorption anti-explosion pot |
| US20150027434A1 (en) * | 2012-02-21 | 2015-01-29 | Anthrogenesis Corporation | Devices and methods for thawing biological material |
| CN204564162U (en) * | 2015-04-17 | 2015-08-19 | 重庆工业职业技术学院 | Protected against explosion weighs safety device |
| CN205011771U (en) * | 2015-09-25 | 2016-02-03 | 云南中烟工业有限责任公司 | Explosion -proof egg of cell recovery |
-
2015
- 2015-09-25 CN CN201510622688.1A patent/CN105087382B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202401064U (en) * | 2011-12-29 | 2012-08-29 | 郑州伊美诺生物技术有限公司 | Suspension resuscitation device applicable to quick resuscitation of cells |
| US20150027434A1 (en) * | 2012-02-21 | 2015-01-29 | Anthrogenesis Corporation | Devices and methods for thawing biological material |
| CN104061829A (en) * | 2014-06-03 | 2014-09-24 | 北京空间飞行器总体设计部 | Portable and deformable energy adsorption anti-explosion pot |
| CN204564162U (en) * | 2015-04-17 | 2015-08-19 | 重庆工业职业技术学院 | Protected against explosion weighs safety device |
| CN205011771U (en) * | 2015-09-25 | 2016-02-03 | 云南中烟工业有限责任公司 | Explosion -proof egg of cell recovery |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108277152A (en) * | 2018-04-04 | 2018-07-13 | 徐俊 | The sterile liquid changing device of cell recovery and carry out the method that cell recovery changes liquid using the device |
| CN108277152B (en) * | 2018-04-04 | 2023-10-13 | 博品(上海)生物医药科技有限公司 | Sterile liquid exchange device for cell resuscitation and method for carrying out cell resuscitation liquid exchange by using device |
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| Publication number | Publication date |
|---|---|
| CN105087382B (en) | 2017-07-04 |
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