CN105087382B - A kind of explosion-proof egg of cell recovery - Google Patents
A kind of explosion-proof egg of cell recovery Download PDFInfo
- Publication number
- CN105087382B CN105087382B CN201510622688.1A CN201510622688A CN105087382B CN 105087382 B CN105087382 B CN 105087382B CN 201510622688 A CN201510622688 A CN 201510622688A CN 105087382 B CN105087382 B CN 105087382B
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- China
- Prior art keywords
- explosion
- cell recovery
- cell
- half eggshell
- upper half
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/20—Heating; Cooling
Abstract
The present invention relates to a kind of explosion-proof egg of cell recovery, it includes the lower half eggshell (1) that can be combined together and upper half eggshell (2)；The bottom of wherein described lower half eggshell (1) is lead bottom (4), has stomata (5) and sample feeding pipe (3) on the upper half eggshell (2).It is the explosion-proof egg simple structure of cell recovery of the invention, easy to use, solve the problems, such as that cell cryopreservation tube blast can be damaged to testing crew during cell recovery；Meanwhile, the design at lead bottom enables that the explosion-proof egg of the cell recovery is rocked as " tumbler ", so that cell cryopreservation tube is heated evenly, it is ensured that cell recovery effect.
Description
Technical field
The invention belongs to cell recovery engineering device technique field, and in particular to a kind of explosion-proof egg of cell recovery.
Background technology
In the experimental implementation related to cell, often cell is frozen and recovered.Cell recovery is with culture
Important working foundation in Evaluation of Biocompatibility system.The principle of cell recovery is fast melt, it is necessary to will be frozen -196
Cell fast melt under DEG C liquid nitrogen or other low temperature environments makes ice crystal during extracellular freezing melt rapidly to 37 DEG C, it is to avoid
Enter cell when ice crystal slowly melts and form recrystallization, cell is caused damage, especially cell is easier to be broken at 0-5 DEG C
It is bad, so the cell for freezing must be made most to spend this temperature soon.
The operating process of cell recovery is usually：Cell cryopreservation tube is taken out under liquid nitrogen or other low temperature environments, is directly soaked
In entering 37 DEG C of warm water, and shake makes it melt as early as possible frequently.Cell cryopreservation tube is then taken out from 37 DEG C of water-baths again, lid is opened
Son, cell suspension is suctioned out with suction pipe.In order that cell cryopreservation tube is heated evenly in cell recovery, reduces ice crystal and occur, generally
During cell recovery, experimenter needs to be held cell cryopreservation tube and be placed in 37 DEG C of water-baths and rock the cell with hand to freeze
Deposit pipe.But due to that may have liquid nitrogen to enter in cell cryopreservation tube, liquid nitrogen gasifies rapidly and causes during being rapidly heated
Cell cryopreservation tube can be exploded, although general experimenter can be protected eyes with goggles, but other portions of body
Position especially exposing cell cryopreservation tube hand generally only have generic latex gloves protection, can cause in cell cryopreservation tube explosion time
Emgloves ruptures, and the slight injured situation of experimenter's hand often occurs.
In order to solve cell cryopreservation tube explosion issues, the scheme for generally using is to clamp cell cryopreservation tube in water with tweezers
Rock, while take muffler protected, but the method operation more inconvenience, and still there are body other parts and hindered
Harmful possibility.Also part test personnel just cover water-bath with lid immediately after using and for cell cryopreservation tube being placed in water-bath
Method, but because general conventional water-bath is all without swirling device, if not shaking cell cryopreservation tube, it is easily heated
It is uneven and influence cell recovery effect, and it is still unavoidable from the blast occurred when cell cryopreservation tube has just been placed in into water-bath.
In order to solve the above problems, the present invention is proposed.
The content of the invention
It is an object of the invention to provide a kind of explosion-proof egg of cell recovery, to solve the cell cryopreservation tube during cell recovery
The problem of uneven influence cell recovery effect that blast is damaged on testing crew and cell cryopreservation tube is heated.
First aspect present invention is related to a kind of explosion-proof egg of cell recovery, and it includes the He of lower half eggshell 1 that can be combined together
Upper half eggshell 2；The bottom of wherein described lower half eggshell 1 is lead bottom 4, has stomata 5 and sample feeding pipe 3 on the upper half eggshell 2.
In a preferred embodiment of the present invention, by the band that is flexible coupling between the lower half eggshell 1 and the upper half eggshell 2
6 connections.
In a preferred embodiment of the present invention, can engage or lead between the lower half eggshell 1 and the upper half eggshell 2
Cross threaded connection.
In a preferred embodiment of the present invention, the quantity of the sample feeding pipe 3 is two.
In a preferred embodiment of the present invention, the length of the sample feeding pipe 3 is 0-3cm.
In a preferred embodiment of the present invention, the stomata 5 is located at the top of the upper half eggshell 2, the stomata 5
Upper end is network structure.The outlet for functioning as the gas in the explosion-proof egg of the cell recovery of the stomata 5, the stomata 5
Preferably fine mesh structure, to prevent the object in the explosion-proof egg of cell recovery from spilling.
In a preferred embodiment of the present invention, when the lower half eggshell 1 and the upper half eggshell 2 are combined together,
Both also have sealing ring in junction periphery.
Device of the invention is an egg type cell recovery device, and it uses process as follows：Separate the He of lower half eggshell 1
The upper half eggshell 2, to the warm water 8 that 37-39 DEG C is injected in the lower half eggshell 1, covers the upper half eggshell 2；By the device
At the liquid nitrogen container to freeze-stored cell, then cell cryopreservation tube 7 is taken out from liquid nitrogen, the sample introduction from the upper half eggshell 2
The explosion-proof egg of cell recovery is put into pipe 3, and gives the cell recovery one initial power of explosion-proof egg, make its as " tumbler "
Rocked naturally on testing stand, being opened after 5min by the upper half eggshell 2 takes out the cell cryopreservation tube 7 carries out next step examination
Test.
Second aspect present invention is related to the explosion-proof egg of the cell recovery described in first aspect present invention for the side of cell recovery
Method, it is comprised the following steps：
A. the lower half eggshell (1) and the upper half eggshell (2) are separated, to 37-39 DEG C of injection in the lower half eggshell (1)
Warm water (8), cover the upper half eggshell (2)；
B. cell cryopreservation tube (7) is taken out from liquid nitrogen, this is put into the sample feeding pipe (3) from the upper half eggshell (2)
In the explosion-proof egg of cell recovery, and the cell recovery one initial thrust of explosion-proof egg is given, it is rocked naturally carries out cell recovery.
Third aspect present invention is related to the explosion-proof egg of the cell recovery described in first aspect present invention for the use of cell recovery
On the way.
In a preferred embodiment of the present invention, cell cryopreservation tube is quick-fried during the purposes is for preventing cell recovery
It is fried.
The present invention achieves following beneficial effect：
It is the explosion-proof egg simple structure of cell recovery of the invention, easy to use, solve the cell during cell recovery and freeze
Deposit the problem that pipe blast can be damaged to testing crew；Meanwhile, the design at lead bottom enables the explosion-proof egg of the cell recovery as " no
Father-in-law " equally rocks, so that cell cryopreservation tube is heated evenly, it is ensured that cell recovery effect.
Brief description of the drawings
Fig. 1 is the explosion-proof egg overall schematic of cell recovery of the invention.
Fig. 2 is schematic diagram after the explosion-proof egg of cell recovery of the invention is opened.
Wherein each reference has following meanings：
1- lower half eggshells；2- upper half eggshells；3- sample feeding pipes；4- lead bottom；5- stomatas；6- is flexible coupling band；7- cell cryopreservation tubes；
8- warm water.
Specific embodiment
The present invention is explained in further detail with reference to embodiment and accompanying drawing, when it should be understood that embodiment is only to show
Example property, and it is nonrestrictive.
Embodiment 1
The explosion-proof egg of cell recovery as depicted in figs. 1 and 2, it includes the lower half eggshell 1 that can be combined together and upper half egg
Shell 2；The bottom of wherein described lower half eggshell 1 is lead bottom 4, has 1 stomata 5 and 2 sample feeding pipes 3 on the upper half eggshell 2.Institute
State and connected by the band 6 that is flexible coupling between lower half eggshell 1 and the upper half eggshell 2, the stomata 5 is located at the upper half eggshell 2
Top.Wherein described sample feeding pipe 3 has certain length, its purpose is to prevent the explosion time of cell cryopreservation tube 7, water or other things
Matter is from middle ejection on the sample feeding pipe 3.
The use process of the device is as follows：The lower half eggshell 1 and the upper half eggshell 2 are separated, to the lower half eggshell 1
The warm water 8 of middle 37-39 DEG C of injection, covers the upper half eggshell 2；The device is taken to the liquid nitrogen container of freeze-stored cell, then will
Cell cryopreservation tube 7 is taken out from liquid nitrogen, and the explosion-proof egg of cell recovery is put into the sample feeding pipe 3 from the upper half eggshell 2, and to this
Cell recovery one initial power of explosion-proof egg, makes it be rocked naturally on testing stand as " tumbler ", and institute is opened after 5min
Stating by upper half eggshell 2 takes out the cell cryopreservation tube 7 carries out next step experiment.
It is the explosion-proof egg simple structure of cell recovery of the invention, easy to use, there is very strong practicality in cell recovery field
Property.
Claims (10)
1. the explosion-proof egg type device of a kind of cell recovery, it is characterised in that it includes the lower half eggshell (1) that can be combined together and upper
Half eggshell (2)；The bottom of wherein described lower half eggshell (1) is lead bottom (4), has stomata (5) on the upper half eggshell (2) and enters
Sample pipe (3), sample feeding pipe (3) is for cell cryopreservation tube (7) to be put into explosion-proof egg type device.
2. the explosion-proof egg type device of cell recovery according to claim 1, it is characterised in that the lower half eggshell (1) and institute
State and connected by the band (6) that is flexible coupling between upper half eggshell (2).
3. the explosion-proof egg type device of cell recovery according to claim 1, it is characterised in that the lower half eggshell (1) and institute
State and can engage between upper half eggshell (2) or be connected through a screw thread.
4. the explosion-proof egg type device of cell recovery according to claim 1, it is characterised in that the quantity of the sample feeding pipe (3)
It is two.
5. the explosion-proof egg type device of cell recovery according to claim 1 or 4, it is characterised in that the length of the sample feeding pipe (3)
It is 0-3cm to spend.
6. the explosion-proof egg type device of cell recovery according to claim 1, it is characterised in that the stomata (5) is positioned at described
The top of upper half eggshell (2), the upper end of the stomata (5) is network structure.
7. the explosion-proof egg type device of cell recovery according to claim 1 or 3, it is characterised in that when the lower half eggshell (1)
When being combined together with the upper half eggshell (2), both junctions periphery also has sealing ring.
8. the method that the explosion-proof egg type device of cell recovery described in claim 1 is used for cell recovery, it is characterised in that it includes
Following steps：
A. the lower half eggshell (1) and the upper half eggshell (2) are separated, to the temperature of 37-39 DEG C of injection in the lower half eggshell (1)
Water (8), covers the upper half eggshell (2)；
B. cell cryopreservation tube (7) is taken out from liquid nitrogen, the cell is put into the sample feeding pipe (3) from the upper half eggshell (2)
Recover in explosion-proof egg, and give the cell recovery one initial thrust of explosion-proof egg, it is rocked naturally carries out cell recovery.
9. the purposes of the explosion-proof egg type device of cell recovery described in claim 1, it is characterised in that, it can be used for cell recovery.
10. purposes according to claim 9, it is characterised in that it is used to prevent cell cryopreservation tube during cell recovery
Blast.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510622688.1A CN105087382B (en) | 2015-09-25 | 2015-09-25 | A kind of explosion-proof egg of cell recovery |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510622688.1A CN105087382B (en) | 2015-09-25 | 2015-09-25 | A kind of explosion-proof egg of cell recovery |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105087382A CN105087382A (en) | 2015-11-25 |
| CN105087382B true CN105087382B (en) | 2017-07-04 |
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ID=54568720
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510622688.1A Active CN105087382B (en) | 2015-09-25 | 2015-09-25 | A kind of explosion-proof egg of cell recovery |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108277152B (en) * | 2018-04-04 | 2023-10-13 | 博品(上海)生物医药科技有限公司 | Sterile liquid exchange device for cell resuscitation and method for carrying out cell resuscitation liquid exchange by using device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202401064U (en) * | 2011-12-29 | 2012-08-29 | 郑州伊美诺生物技术有限公司 | Suspension resuscitation device applicable to quick resuscitation of cells |
| CN204564162U (en) * | 2015-04-17 | 2015-08-19 | 重庆工业职业技术学院 | Protected against explosion weighs safety device |
| CN205011771U (en) * | 2015-09-25 | 2016-02-03 | 云南中烟工业有限责任公司 | Explosion -proof egg of cell recovery |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013126379A1 (en) * | 2012-02-21 | 2013-08-29 | Anthrogenesis Corporation | Devices and methods for thawing biological material |
| CN104061829B (en) * | 2014-06-03 | 2015-05-27 | 北京空间飞行器总体设计部 | Portable and deformable energy adsorption anti-explosion pot |
-
2015
- 2015-09-25 CN CN201510622688.1A patent/CN105087382B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202401064U (en) * | 2011-12-29 | 2012-08-29 | 郑州伊美诺生物技术有限公司 | Suspension resuscitation device applicable to quick resuscitation of cells |
| CN204564162U (en) * | 2015-04-17 | 2015-08-19 | 重庆工业职业技术学院 | Protected against explosion weighs safety device |
| CN205011771U (en) * | 2015-09-25 | 2016-02-03 | 云南中烟工业有限责任公司 | Explosion -proof egg of cell recovery |
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| Publication number | Publication date |
|---|---|
| CN105087382A (en) | 2015-11-25 |
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