CN105087362A - Gravity self-adaptive ruling mechanism - Google Patents

Gravity self-adaptive ruling mechanism Download PDF

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Publication number
CN105087362A
CN105087362A CN201510630561.4A CN201510630561A CN105087362A CN 105087362 A CN105087362 A CN 105087362A CN 201510630561 A CN201510630561 A CN 201510630561A CN 105087362 A CN105087362 A CN 105087362A
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China
Prior art keywords
lock sleeve
coupling end
inboardend
sleeve
fit
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CN201510630561.4A
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CN105087362B (en
Inventor
何林声
赵勇刚
袁军
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Chongqing Kejie Medical Technology Co., Ltd
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Dao Xin Bio Tech Ltd Chongqing
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

To solve the problems of manual ruling in the prior art that the biological safety is low, the operation is non-standard, the randomness of manual operation is large, the labor cost is high, the efficiency is low, and a culture medium is easy to scratch, the invention provides a gravity self-adaptive ruling mechanism which comprises a fixing end, a locking sleeve, a connecting end and a ruling pen, wherein the fixing end adopts a rigid structure; the top of the fixing end is adaptive to an external mechanical moving mechanism; the top of the locking sleeve adopts an upwards opening sleeve structure, and is fixed to the bottom of the fixing end in a coordinated manner; the bottom of the locking sleeve adopts a downwards opening sleeve structure; a platform bulging in the inner diameter direction is arranged at the bottom of the locking sleeve; a positioning block of which the section is T-shaped is arranged at the top of the connecting end; the top of the connecting end is in positioning coordination with the platform; the bottom of the locking sleeve is also in interference fit with the rod part of the connecting end; an opening is formed in the top of the ruling pen, and tightly clamps the bottom of the connecting end. The work efficiency is high, the operating process is standardized and mechanized, and the culture medium is prevented from being scratched.

Description

A kind of gravity self-adaptation liner agency
Technical field
The present invention relates to a kind of gravity self-adaptation liner agency.
Background technology
Liner agency is very important apparatus in microbial inoculant, cultivation, separation and detection.At present, microbial inoculant, cultivation, separation and detection all adopt manual method to carry out, and when artificial line, its concrete operation step is as follows:
(1) laboratory technician's hand-held sampling ring carries out artificial sterilizing;
(2) laboratory technician picks micro-biological samples, and annotates on the surface of glass substratum gently by a small amount of micro-biological samples;
(3) in media surface line, thus the different cells in microorganism mixed in together or same microbial population are obtained the individual cells of more independent distribution by sectional streak dilution, after cultivating, growth and breeding becomes single bacterium colony.
Obviously, existing artificial scribble method has following shortcoming:
(1) when operating, testing staff is according to the sensation of hand, and make the end of transfering loop just touch the surface of substratum, do not have stability, the arbitrariness of line is higher;
(2) due to adopt manual, make transfering loop just touch the surface of substratum with feeling, but, time and contact substratum surperficial time and the phenomenon left is difficult to avoid, easy cross infection or bacteria distribution poor effect;
(3) owing to adopting artificial hatched manner, cause result can not stdn, experimental result recognizes difficulty again, judges inaccurate;
(4) manual operation is wasted time and energy, inefficiency;
(5) have potential biological pollution and hazardness, operator easily come to harm in open experimental situation.
Moreover, time more crucial, the liner agency of prior art by the contact surface of the adaptive adjustment of gravity and substratum, easily cannot scratch substratum.
Therefore, the artificial line of prior art also exist that biological safety is low, operation can not stdn, manual operation is random large, human cost is high and the problem that inefficiency, substratum easily scratch.
Summary of the invention
Manually rule that the biological safety that exists is low for solving prior art, operation can not stdn, manual operation is random large, human cost is high and inefficiency, substratum such as easily to scratch at the problem, the present invention proposes a kind of gravity self-adaptation liner agency.
Gravity self-adaptation liner agency of the present invention, comprises the inboardend, lock sleeve, coupling end and the bow pen that arrange from top to bottom, and described inboardend is rigid structure, and its top and external mechanical motion mechanism adaptation; The top of described lock sleeve is the sleeve-like structure of upward opening, and with the bottom heavy keying fit of inboardend, the bottom of lock sleeve is the sleeve-like structure to lower opening, and bottom arranges the platform to internal diameter direction projection; The top of described coupling end is section T-shaped preset pieces, and with platform location fit, the bottom of described lock sleeve also with the bar portion shrink-fit of coupling end; The top of described bow pen arranges perforate, and the bottom of shown perforate and coupling end is stuck.
Or gravity self-adaptation liner agency of the present invention, comprise the inboardend, lock sleeve, coupling end and the bow pen that arrange from top to bottom, described inboardend is rigid structure, and its top and external mechanical motion mechanism adaptation; The top of described lock sleeve is the sleeve-like structure of upward opening, and crosses heavy keying fit with the bottom of inboardend, and the bottom of lock sleeve is the sleeve-like structure to lower opening, and bottom arranges the platform to internal diameter direction projection; The top profile of described coupling end is T-shaped preset pieces, and with platform location fit, the bottom of described lock sleeve also with the bar portion shrink-fit of coupling end; What described bow pen and coupling end were integrated is fixedly connected with.
Further, described lock sleeve top and the heavy keying fit mode bottom inboardend are that thread fit or latch coordinate.
Further, namely the bottom surface of the bottom line end of described bow pen can be cambered surface or plane with the contact face of line of substratum.
Further, described bow pen is rigid structure or flexible structure.
The Advantageous Effects of gravity self-adaptation liner agency of the present invention is: biosafety issues is solved; Protection environment and operator; operational standardization, human cost reduces, and working efficiency increases, operating process standardized mechanical, gravity Automatic adjusument do not scratch substratum.
Accompanying drawing explanation
Accompanying drawing 1 is the structural representation of gravity self-adaptation liner agency of the present invention;
Accompanying drawing 2 is the cross-sectional view of gravity self-adaptation liner agency of the present invention;
Below in conjunction with the drawings and specific embodiments, gravity self-adaptation liner agency of the present invention is further described.
Embodiment
Accompanying drawing 1 is the structural representation of gravity self-adaptation liner agency of the present invention, accompanying drawing 2 is the cross-sectional view of gravity self-adaptation liner agency of the present invention, in figure, 1 be inboardend, 2 for lock sleeve, 3 for coupling end, 4 for bow pen, 5 for platform, 6 for preset pieces, 7 for perforate, 8 for line end, 9 is through hole, as shown in the figure, gravity self-adaptation liner agency of the present invention, comprise the inboardend 1, lock sleeve 2, coupling end 3 and the bow pen 4 that arrange from top to bottom, described inboardend 1 is rigid structure, and its top and external mechanical motion mechanism adaptation; The top of described lock sleeve 2 is the sleeve-like structure of upward opening, and with the bottom heavy keying fit of inboardend 1, the bottom of lock sleeve 2 is the sleeve-like structure to lower opening, and bottom arranges the platform 5 to internal diameter direction projection; The top of described coupling end 3 is section T-shaped preset pieces 6, and with platform 5 location fit, the bottom of described lock sleeve 2 also with the bar portion shrink-fit of coupling end 3; The top of described bow pen 4 arranges perforate 7, and shown perforate 7 is stuck with the bottom of coupling end 3.
Or gravity self-adaptation liner agency of the present invention, comprise the inboardend 1, lock sleeve 2, coupling end 3 and the bow pen 4 that arrange from top to bottom, described inboardend 1 is rigid structure, and its top and external mechanical motion mechanism adaptation; The top of described lock sleeve 2 is the sleeve-like structure of upward opening, and with the bottom heavy keying fit of inboardend 1, the bottom of lock sleeve 2 is the sleeve-like structure to lower opening, and bottom arranges the platform 5 to internal diameter direction projection; The top profile of described coupling end 3 is T-shaped preset pieces 6, and with platform 5 location fit, the bottom of described lock sleeve 2 also with the bar portion shrink-fit of coupling end 3; What described bow pen 4 was integrated with coupling end 3 is fixedly connected with.
Be that thread fit or latch coordinate in order to make inboardend 1 and lock sleeve 2 better locked cooperation described lock sleeve 2 top with the heavy keying fit mode bottom inboardend 1, in the specific implementation, bottom described inboardend 1, outside screw is set, the inwall of the top shell-like of described lock sleeve 2 arranges internal thread, the bottom outside screw of described inboardend 1 and the top internal thread of lock sleeve 2 meshing.Or adopt all intelligible latch mode of those skilled in the art to carry out coordinating.
It will be understood by those skilled in the art that, when the mode of connection of coupling end 3 and bow pen 4 adopt be flexibly connected time, in order to coupling end 3 can better insert in the perforate at bow pen 4 top, in the perforate at bow pen 4 top under a bottom outline of described coupling end 3, conveniently insert.
In order to better line, and making bow pen can touch media surface more smoothly, meanwhile, not scratching substratum when ruling, namely the bottom surface of the bottom line end of described bow pen can be cambered surface or plane with the contact face of line of substratum.It will be appreciated by persons skilled in the art that cambered surface, then the line end 8 of bow pen can be ball, semi-round ball, annulus, oval ball, bowl-shape state etc.Plane, then the line end 8 of bow pen can for being parallel to the trilateral of horizontal plane, rectangle, the shapes such as square.
In the specific implementation, coupling end 3 with bow pen 4 for being fixedly connected with time, described bow pen 4 is rigid structure; When coupling end 3 and bow pen 4 are dismountable flexible connection, described bow pen 4 is flexible structure.
It will be understood by those skilled in the art that, when lock sleeve 2 and coupling end 3 shrink-fit, positioned with the platform 5 being arranged at lock sleeve 2 bottom opening place by the T-shaped preset pieces 6 being arranged at coupling end 3 top, when contacting media surface, owing to there being the cavity of hollow in the middle part of lock sleeve 2, so bow pen can be contacted with substratum gently according to self gravitation and not scratch substratum.When the level attitude of media surface improves, coupling end 3 automatically indentation cavity, when the level attitude of media surface reduces, coupling end 3 skids off a segment distance automatically, and therefore can not drop out lock sleeve 2 due to T-shaped preset pieces 6.In the specific implementation, if the top diameter of coupling end 3 differs very small with lock sleeve 2 bottom inner diameter, then when stretching back and forth, air is not easy to get rid of cavity, be unfavorable for the flexible of coupling end 3 after air sharply compresses, therefore the cavity portion of described lock sleeve 2 through hole 9 can be set, for exhaust.Meanwhile, when implementing, if the difference of the top diameter of coupling end 3 and lock sleeve 2 bottom inner diameter increases, then air overflows by gap, then now do not need to arrange through hole 9.
When operating, namely coupling end 3 and the mode of connection of bow pen 4 can be that to be fixedly connected with also can be dismountable flexible connection.No matter adopt which kind of mode, all can not affect the line of line end on substratum.Owing to ruling, the bottom sectional of end 8 is cambered surface, and end of therefore ruling can be spherical shape or hemispherical, thus the better line effect of realization to media surface.It will be appreciated by persons skilled in the art that when adopting plane, with media surface contact deleterious, and more easily to scratch, therefore not recommend the mode adopting plane.
Obviously; gravity self-adaptation liner agency biosafety issues of the present invention is solved, Protection environment and operator, operational standardization; human cost reduces, and working efficiency increases, operating process standardized mechanical, gravity Automatic adjusument do not scratch substratum.

Claims (5)

1. a gravity self-adaptation liner agency, it is characterized in that, comprise the inboardend (1), lock sleeve (2), coupling end (3) and the bow pen (4) that arrange from top to bottom, described inboardend (1) is rigid structure, and its top and external mechanical motion mechanism adaptation; The top of described lock sleeve (2) is the sleeve-like structure of upward opening, and with the bottom heavy keying fit of inboardend (1), the bottom of lock sleeve (2) is the sleeve-like structure to lower opening, and bottom arranges the platform (5) to internal diameter direction projection; The top of described coupling end (3) is section T-shaped preset pieces (6), and with platform (5) location fit, the bottom of described lock sleeve (2) also with the bar portion shrink-fit of coupling end (3); The top of described bow pen (4) arranges perforate (7), and shown perforate (7) coordinates with the bottom of coupling end (3) is stuck.
2. a gravity self-adaptation liner agency, it is characterized in that, comprise the inboardend (1), lock sleeve (2), coupling end (3) and the bow pen (4) that arrange from top to bottom, described inboardend (1) is rigid structure, and its top and external mechanical motion mechanism adaptation; The top of described lock sleeve (2) is the sleeve-like structure of upward opening, and with the bottom heavy keying fit of inboardend (1), the bottom of lock sleeve (2) is the sleeve-like structure to lower opening, and bottom arranges the platform (5) to internal diameter direction projection; The top profile of described coupling end (3) is T-shaped preset pieces (6), and with platform (5) location fit, the bottom of described lock sleeve (2) also with the bar portion shrink-fit of coupling end (3); What described bow pen (4) was integrated with coupling end (3) is fixedly connected with.
3. gravity self-adaptation liner agency according to claim 1 and 2, is characterized in that, described lock sleeve (2) top is that thread fit or latch coordinate with the heavy keying fit mode of inboardend (1) bottom.
4. gravity self-adaptation liner agency according to claim 1 and 2, is characterized in that, the bottom surface of bottom line end (8) of described bow pen (4) namely can be cambered surface or plane with the contact face of line of substratum.
5. gravity self-adaptation liner agency according to claim 1 and 2, is characterized in that, described bow pen 4 is rigid structure or flexible structure.
CN201510630561.4A 2015-09-29 2015-09-29 A kind of adaptive liner agency of gravity Active CN105087362B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114317246A (en) * 2022-01-13 2022-04-12 济南市疾病预防控制中心 Microorganism seed taking and marking device and method

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3742187A (en) * 1970-10-22 1973-06-26 Becton Dickinson Co Inoculation and transfer loop and sterilizer
CN201190162Y (en) * 2008-03-10 2009-02-04 董炳刚 Bacterial taking device
CN201587941U (en) * 2009-11-17 2010-09-22 曲良才 Novel bacterination device
CN202114724U (en) * 2011-06-15 2012-01-18 山东潍坊福田模具有限责任公司 Elastic marking scriber
CN202925020U (en) * 2012-11-08 2013-05-08 浙江出入境检验检疫局检验检疫技术中心 Bacterial block inoculator
CN204369888U (en) * 2014-12-04 2015-06-03 济南朗迈生物科技有限公司 A kind of culture medium inoculated device utilizing gravity control dynamics
CN104726327A (en) * 2015-04-15 2015-06-24 重庆道鑫生物科技有限公司 Microorganism scriber
CN205077052U (en) * 2015-09-29 2016-03-09 重庆道鑫生物科技有限公司 Gravity self -adaptation marking off mechanism

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3742187A (en) * 1970-10-22 1973-06-26 Becton Dickinson Co Inoculation and transfer loop and sterilizer
CN201190162Y (en) * 2008-03-10 2009-02-04 董炳刚 Bacterial taking device
CN201587941U (en) * 2009-11-17 2010-09-22 曲良才 Novel bacterination device
CN202114724U (en) * 2011-06-15 2012-01-18 山东潍坊福田模具有限责任公司 Elastic marking scriber
CN202925020U (en) * 2012-11-08 2013-05-08 浙江出入境检验检疫局检验检疫技术中心 Bacterial block inoculator
CN204369888U (en) * 2014-12-04 2015-06-03 济南朗迈生物科技有限公司 A kind of culture medium inoculated device utilizing gravity control dynamics
CN104726327A (en) * 2015-04-15 2015-06-24 重庆道鑫生物科技有限公司 Microorganism scriber
CN205077052U (en) * 2015-09-29 2016-03-09 重庆道鑫生物科技有限公司 Gravity self -adaptation marking off mechanism

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114317246A (en) * 2022-01-13 2022-04-12 济南市疾病预防控制中心 Microorganism seed taking and marking device and method
CN114317246B (en) * 2022-01-13 2024-02-20 济南市疾病预防控制中心 Microorganism seed taking and scribing device and method

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Address after: 400700 No. 384 Beijing Oriental Avenue, Beibei District, Chongqing

Patentee after: Chongqing Qu Yuan Medical Technology Co., Ltd.

Address before: Building 9-1, Building A, 55 Gang'an Road, Jiangbei District, Chongqing 400025

Patentee before: Dao Xin bio tech ltd, Chongqing

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Address after: No. 384, Chongqing, Beibei District, Chongqing

Patentee after: Chongqing Kejie Medical Technology Co., Ltd

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