CN204474671U - Combined manual microbial inoculant device - Google Patents
Combined manual microbial inoculant device Download PDFInfo
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- CN204474671U CN204474671U CN201520144766.7U CN201520144766U CN204474671U CN 204474671 U CN204474671 U CN 204474671U CN 201520144766 U CN201520144766 U CN 201520144766U CN 204474671 U CN204474671 U CN 204474671U
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Abstract
The utility model relates to microbial inoculant equipment, refers to a kind of Combined manual microbial inoculant device especially.Comprise head rod, afterbody and the removable fixing assembling of head rod and in the comb teeth-shaped inoculation head on head rod spaced apart, the inoculation pitch of holes adaptation that the spacing of inoculation head and porous culture plate distribute.The utility model solves the problems such as the inoculation speed of prior art existence is slow, microbiological contamination rate is high.There is the advantages such as inoculation speed is fast, microbiological contamination rate is low, structure is simple, operation is simple.
Description
Technical field
The utility model relates to microbial inoculant equipment, refers to a kind of Combined manual microbial inoculant device especially.
Background technology
Fungi preservation is very important link in microorganism career field, and the object that microbial strains preserves is the long period to keep the activity of bacterial classification, and heredity, stability on physiology and morphology.Therefore the stable of microorganism germline is kept to be ensure experiment and produce the prerequisite of carrying out smoothly.
Preiservative methods has the multiple methods such as solid, semisolid and fluid preservation.Wherein solid preservation be the most frequently used in culture presevation, operate method eaily.At present, for the microorganism that can adopt solid method for preserving, the solid protecting method that laboratory or factory commonly use is for going down to posterity in dull and stereotyped or test tube slant, schedule of operation, for making the dull and stereotyped or slant medium of conservation, then utilizes transfering loop, inoculating needle and loop-carrier by the bacterial classification flat board being transferred to new configuration one by one or test tube on aseptic operating platform.This forwarding method workload for a small amount of bacterial classification is lower, but for the unit having a large amount of bacterial classification to need preservation, or at the initial stage of bacterial screening, due to bacterial classification enormous amount (as hundreds of even several thousand strain bacterium up to ten thousand), above-mentioned working efficiency of transferring one by one is extremely low, and the time of meeting at substantial and manpower, what is more important, along with the prolongation of inoculation time, add the chance of bacteria infection in seeded process, this also exists critical defect for microbial cultivation process simultaneously.Therefore for not having aerial hyphae, the microorganism (comprise photosynthetic and heterotrophic microalgae cell) of smooth colony can being formed at planar surface, a kind of can the classification inoculation apparatus simply and easily of disposable switching multi-cultur es being very important is designed.
The classification inoculation apparatus great majority reported in current existing document are single-point classification inoculation apparatus, minority classification inoculation apparatus is wherein had to adopt Electronic control and utilize the mode of multiple-station rotation to work continuously, but said structure Problems existing one is complex structure and cost of manufacture is higher, although two is can to a certain degree improve inoculation speed, but still fundamentally cannot solve the slow-footed problem of inoculation, and gnotobasis required in seeded process is difficult to obtain effective guarantee.
Utility model content
The purpose of this utility model is that providing a kind of is applicable to existing porous culture plate and the Combined manual microbial inoculant device of multiple bacterial classification of transferring fast.
Overall technology design of the present utility model is:
Combined manual microbial inoculant device, comprises head rod, afterbody and the removable fixing assembling of head rod and in the comb teeth-shaped inoculation head on head rod spaced apart, the inoculation pitch of holes adaptation that the spacing of inoculation head and porous culture plate distribute.
Can it is evident that, the shape of head rod can adopt various structures design according to the quantity and distribution porous culture plate being inoculated hole, comprising but be not limited to adopt shaft-like, the structure formations such as the compound rod of rectangle or parallel interval arrangement, when the culture hole on culture plate is single distribution, head rod can adopt shaft-like, but culture plate be rectangle and its surface arrangement is the culture hole of lattice-like time, then adopt rectangle head rod corresponding with it, the structure formation that corresponding inoculation head can adopt the multiple row comb teeth-shaped corresponding with the culture hole that lattice-like distributes to distribute, do not depart from technology category of the present utility model.
Concrete technical conceive of the present utility model also has:
For ease of the gripping of operator and the needs of operation, preferred structural implementations also comprises handle, and handle, at its lower end and head rod connect.
The requirement with quick assembling is fixed for meeting handle, realize the microorganism switching to the culture plate with lattice-like culture hole, preferred technical implementation way is, also comprises the second union lever, the second union lever is first-class be intervally distributed with handle upper end adaptation be connected and fixed structure.According to requirements of one's work, multiple combination mode can be selected flexibly, the head rod and the second union lever with handle fixed like this.
For ensureing the realization of the rapid-assembling in operation of each component in the utility model, meet the connection reliability between adjacent zeros parts simultaneously, preferred technical implementation way adopts the removable mode be fixedly connected with, between described inoculation head and head rod, between handle, at its lower end and head rod and between the second union lever and handle upper end including, but not limited to adopting screw threads for fastening, plug-in mounting is fixed, it is fixing to clamp, be set with fix, one in screw fastening, bolted or its combine.Wherein comparatively preferred and easy implementation adopts screw fastening assembling.
For realizing quick assembling further, realize not easily producing radial rotating after handle is fixed on the second union lever, the culture hole met on each inoculation head and culture plate is adaptive more accurately simultaneously.Preferred technical implementation way is, the second described union lever and handle upper end are adopted and be slidably matched and fixed by jackscrew.
More preferred technical implementation way is, the second described union lever and handle upper end adopt key to join merga pass jackscrew and fix.Can it is evident that and handle upper end can also be processed into rectangular shape not easily producing rotation etc. and be fixed by jackscrew, all not depart from technical spirit of the present utility model.
The utility model in use, can be selected to assemble handle on the second union lever as required, when not needing to inoculate in the inoculation hole had, the inoculation head of correspondence can be pulled down.
For ease of the sterilizing of inoculator in the utility model, meet the requirement of not easily corrosion, in the utility model not easily there is chemical transformation and the making of resistant to elevated temperatures material in selecting of each component at normal temperatures, wherein more preferably adopts stainless steel to make simultaneously.
For the beneficial effects of the utility model are described, applicant carried out following simultaneous test:
Adopt traditional inoculation method one to one, length consuming time, microbiological contamination probability is high.Strain transfer is carried out as utilized 24 porocyte culture plates, if utilize conventional vaccination ring for inoculating tool, before a bacterial classification of often transferring, all need to carry out aseptically process by being grilled thoroughly by ring, then other after ring is cool at spirit lamp, carry out switch over operation, again the bacterium sticked on ring is burnt to death after inoculation, the operating process connecing a kind is like this 60-90 second, and operate complete 24 orifice plates, required time is 24-36 minute.Even if to transfering loop unified sterilizing in advance, the process of a switching kind also needs the time of about 30 seconds, the switching process of 24 orifice plates needs 30 × 24=720 second (12 minutes).If the operation of 96 orifice plates, the operating time will be respectively 96-144 minute and 48 minutes, within the so long operating time, not only can reduce the aseptic working efficiency of aseptic working platform, and owing to often connecing a strain bacterium, flat board all wants folding once, repetitious like this operation, have impact on the flowing of germ-free air flow, the probability of culture plate microbiological contamination also improves thereupon, as the operation of 24 orifice plates, and 5 blocks of plates of often transferring, for general operation personnel, have the microbiological contamination rate of general about 3%.For culture presevation work, the harm of non-directional microbiological contamination likely directly causes the loss of bacterial classification, causes extreme influence to the work in later stage.Therefore, operating time short as far as possible and the least possible dull and stereotyped opening/closing frequency are the possible methods reducing microbiological contamination rate.And adopting inoculator of the present utility model to carry out inoculation operation, the switching of culture plate can complete within 1 minute, and culture plate only needs folding once, shortened the operating time greatly, avoided microbiological contamination.
Technical progress acquired by the utility model is:
1, project organization of the present utility model is simply ingenious, can carry out the textural association of various ways according to the distribution of culture hole in culture plate, applied widely.
2, in the utility model, the cooperation of each component is firm, and without significantly coordinating dead angle, is convenient to inoculate the sterilising treatment (mode as calcination or other high-temperature sterilization) of tandem docking kind device.
3, adopt the utility model to carry out inoculation operation to porous culture plate, inoculation speed is fast, effectively reduces the microbiological contamination rate in seeded process.
4, the utility model adopt Manual structure, cost of manufacture is low, and operating process is simple, be convenient to existing aseptic inoculation platform with the use of.
Accompanying drawing explanation
Fig. 1 is one-piece construction schematic diagram of the present utility model.
Fig. 2 is the right view of Fig. 1.
Reference numeral in accompanying drawing is as follows:
1, jackscrew; 2, the second union lever; 3, handle; 4, head rod; 5, head is inoculated.
Embodiment
Drawings illustrate embodiment of the present utility model; below in conjunction with accompanying drawing, embodiment of the present utility model is described further; but should not be construed as restriction of the present utility model; the content that protection domain of the present utility model is recorded with claim is as the criterion; any according to the equivalent technical elements replacement done by specification sheets, all do not depart from protection domain of the present utility model.
The overall technology structure of the present embodiment is as diagram, comprising head rod 4, afterbody and the removable fixing assembling of head rod 4 and in the comb teeth-shaped inoculation on head rod 4 spaced apart 5, the inoculation pitch of holes adaptation that the spacing of inoculation 5 and porous culture plate distribute.
Also comprise handle 3, handle 3 lower end and head rod 4 connect.
Also comprise the second union lever 2, second union lever 2 first-class be intervally distributed with handle 3 upper end adaptation be connected and fixed structure.
Described inoculation is adopted between 5 and head rod 4, between handle 3 lower end and head rod 4 and is screwed, and the second union lever 2 coordinates with handle 3 upper end key and assembles merga pass jackscrew 1 and fix.
Claims (7)
1. Combined manual microbial inoculant device, it is characterized in that comprising head rod (4), afterbody and the removable fixing assembling of head rod (4) and in the comb teeth-shaped inoculation head (5) on head rod (4) spaced apart, the inoculation pitch of holes adaptation that the spacing of inoculation head (5) and porous culture plate distribute.
2. Combined manual microbial inoculant device according to claim 1, characterized by further comprising handle (3), and handle (3) lower end and head rod (4) connect.
3. Combined manual microbial inoculant device according to claim 2, characterized by further comprising the second union lever (2), the second union lever (2) is first-class be intervally distributed with handle (3) upper end adaptation be connected and fixed structure.
4. the Combined manual microbial inoculant device according to any one of claim 1-3, it is characterized in that between described inoculation head (5) and head rod (4), between handle (3) lower end and head rod (4) and between the second union lever (2) and handle (3) upper end, adopt screw threads for fastening, plug-in mounting is fixed, it is fixing to clamp, be set with fixing, screw fastening, bolted in one or its combine.
5. Combined manual microbial inoculant device according to claim 4, be is characterized in that described the second union lever (2) and handle (3) upper end adopt and is slidably matched and is fixed by jackscrew (1).
6. Combined manual microbial inoculant device according to claim 5, is characterized in that described the second union lever (2) and handle (3) upper end adopt key to join merga pass jackscrew (1) and fix.
7. Combined manual microbial inoculant device according to claim 4, is characterized in that between described inoculation head (5) and head rod (4), adopts screw fastening between handle (3) lower end and head rod (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520144766.7U CN204474671U (en) | 2015-03-13 | 2015-03-13 | Combined manual microbial inoculant device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520144766.7U CN204474671U (en) | 2015-03-13 | 2015-03-13 | Combined manual microbial inoculant device |
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| CN204474671U true CN204474671U (en) | 2015-07-15 |
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| CN201520144766.7U Active CN204474671U (en) | 2015-03-13 | 2015-03-13 | Combined manual microbial inoculant device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104651225A (en) * | 2015-03-13 | 2015-05-27 | 润科生物工程（福建）有限公司 | Combined manual microorganism inoculator |
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- 2015-03-13 CN CN201520144766.7U patent/CN204474671U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104651225A (en) * | 2015-03-13 | 2015-05-27 | 润科生物工程（福建）有限公司 | Combined manual microorganism inoculator |
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