CN105079227A - Application of traditional Chinese medicine composition in preparing medicine for treating radiation-induced pulmonary injury - Google Patents
Application of traditional Chinese medicine composition in preparing medicine for treating radiation-induced pulmonary injury Download PDFInfo
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Abstract
The invention discloses an application of a traditional Chinese medicine composition in preparing a medicine for treating radiation-induced pulmonary injury. The traditional Chinese medicine composition is mainly composed of fructus forsythiae, honeysuckle flowers, isatis root, rheum officinale, pogostemon cablin, rhizoma dryopteris crassirhizomae and rhodiola rosea, has the integral regulation advantages of compound traditional Chinese medicines, can eliminate pathogenic factors, relieve the symptoms, and adjust immunity, and has a remarkable curative effect for treating radiation-induced pulmonary injury proved by the clinical test.
Description
Technical field
The present invention relates to the application of a kind of Chinese medicine composition in preparation radiotherapy injury of lung medicine, belong to field of Chinese herbal.
Background technology
Induced lung injury (radio-pulmonarylesion) is the complication caused owing to carrying out radiotherapy to the malignant tumor of chest, and seeing at most pulmonary carcinoma, breast carcinoma, is secondly the radiotherapy to the esophageal carcinoma, mediastinum malignant tumor.Common clinical manifestation is for having: suffer from thoracic malignant tumors and accept radiocurable medical history and relevant respiratory symptom, symptom-as can after radiotherapy starts 4 ~ 16 weeks occur, most generation in 2 ~ 4 weeks after radiotherapy terminates, minority terminates also can occur in half a year in radiotherapy, be mainly dry cough, few expectorant, dysphagia, uncomfortable in chest, chest pain, severe patient dyspnea, low grade fever, blood leukocytes are normal, and antibacterial therapy is invalid, acute stage, connects irradiated lung field and hears and moist sound of vomiting sound, respiratory harshness; Chronic phase local percussion is voiced sound, and respiratory murmur is low or hear and thin wet sound of vomiting sound.Inspection method mainly contains: chest X-ray, (1) early stage radiation pneumonia: radiotherapy causes pulmonary congestion in early days and oozes out change, and occur that texture increases and strip shadow at the corresponding site of radiation, edge is fuzzyyer, merges mutually gradually time serious.(2) pulmonary fibrosis: radiation pneumonia appears in radioactive area, part absorbs through treatment, but there is obvious fibrosis the later stage in some patients, present radiation area lung marking slightly random, intensive, filament contraction, tractive adjacent tissue is shifted, if mediastinum and trachea are to sick sidesway, diaphragm pull-up, intercostal space narrows, and Pulmonary volume reduces.Healthy area compensatory emphysema.(3) other change: in mediastinum district, radiation causes radioactivity mediastinitis, performance widened mediastinum, and blur margin, merges mutually with the radiation area in lung sometimes; Large area radiotherapy can cause pachynsis pleurae, adhesion, or a small amount of hydrothorax, and interlobar fissure is broadening; Extensive fibrosis causes arterial hypertension, the sign that hilus pulumonis increases and the right heart is got involved.
Illustrate the mechanism of induced lung injury at present not yet completely.It is generally acknowledged, induced lung injury is the result of the cascading of the many cells-factor of multifactor participation, damage relevant with reactive oxygen free radical with target cell, with generation, the expression [Li Fengyu relevant to signal transduction of cytokine, Liu Xiufang, Zhang Jianyu. the progress [J] of induced lung injury mechanism. modern oncology's medical science, 2009,17 (3): 576-578.].The Early manifestation of induced lung injury is radiation pneumonia, within many 1 ~ 3 month after radiotherapy, occur, with tumor necrosis factor (tumornecrosisfactor, TNF), interleukin 1 (interleukin-1, IL-1) and monocyte chemoattractant protein (monocyte
Chemoattractantprotein, MCP) etc. proinflammatory cytokine closely related, particularly TNF and IL-1 [Cao Jingxu, Zhang Xuzhi. cytokine and induced lung injury [J]. foreign medical science radiological medicine nuclear medicine fascicle, 2001,25, (4): 181-185.]
Current doctor trained in Western medicine essential therapeutic arsenals is for using high dose glucocorticoid added with antibiotic; though achieve certain effect; but in some patients curative effect still not good enough [GhafooriP; MarksLB; VujaskovicZ, etal.Radiation-inducedlunginjury.Assessment, management; andprevention.Oncology (WillistonPark) .2008,22 (1): 37-47; Discussion52-53.].Western medical treatment induced lung injury is the major dose-limiting factor of radiotherapy to the chest according to induced lung injury, and without effective remedy measures, therefore the key disposing induced lung injury is prevention.Once generation induced lung injury, Therapeutic Method the most frequently used clinically at present uses adrenocortical hormone combined with antibiotic.Although heavy dose of hormone can temporary transient effective relief of symptoms, suppress the development of pulmonary fibrosis, easily produce many complication.Cytoprotective amifostine can protect normal structure in certain limit ground, but may there is larger toxic and side effects, and expensive.Therefore, the method for control induced lung injury finding more effective low toxicity become the focus of research [Wang Lu. the effectiveness study [J] of therapy of combining Chinese and Western medicine radiation pneumonia. Chinese medicine innovation, 2010,7 (6): 60-61].
Radiotherapy injury of lung doctor trained in Western medicine is other without effective treatment means except using adrenocortical hormone and antibiotic.According to clinical symptoms, induced lung injury is attributable to the traditional Chinese medical science " cough ", " the syndrome of dyspnea ", " consumptive lung disease " category.Lonizing radiation belong to the heresy of the pyretic toxicity of the traditional Chinese medical science, human body body fluid of easily burning, damage human righteousness and cloudy blood.Lonizing radiation cause a disease different from the heresy of general pyretic toxicity, can not follow defensive-qi-nutrient-blood change of disease rule, but direct attack internal organs blood network.Lung being a delicate viscus, the heresy poison invasion and attack lungs of lonizing radiation, lungs are subject to its damage.First, the heresy the most smoked lung yin that burns of pyretic toxicity, contain in deficiency-heat, consumption hinders human righteousness, impairment of both QI and YIN; Secondly, the deficiency of vital energy is unable agitates blood vessels, and do not nourish blood in deficiency of YIN Tianjin, blood is sticky, causes hematogenous blockage, raw in blood stasis, retardance lung network.Deficiency of both QI and YIN, ecchymosis resistance lung, can have influence on lung a surname and send out respectful to fall, towards the function of hundred arteries and veins, can cause that clearing heat in QI system difficulty enters, foul smell difficulty goes out, therefore dyspnea, symptom uncomfortable in chest can appear in induced lung injury patient, severe patient can the death because of respiratory failure.In a word, pyretic toxicity is the Etiological of induced lung injury, and it is the main pathogenesis of this disease that impairment of both QI and YIN and ecchymosis are tied mutually.The traditional Chinese medical science has some superiority in this field.Chinese medicine more holistic adjusts, and can increase radiotherapeutic effect, improve immunologic function while control induced lung injury, and protection hemopoietic system, improves life in patients.Chinese traditional treatment primary disease should follow symptomatic treatment in acute condition, the principle of radical treatment in chronic case, no matter takes stopgap measures, effects a permanent cure or treating both the principal and secondary aspects of a disease when facing card, should according to dissimilar determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs in addition.
Chinese medicine composition of the present invention has antipyretic and detoxicated, effect that lung qi dispersing expels the heat-evil, induce sweat expelling pathogenic factors from the exterior, outer lung qi dispersing of Fructus Forsythiae in side, Flos Lonicerae, Herba Ephedrae (processed), Herba Menthae, Herba Pogostemonis is defended, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Herba Houttuyniae, Radix Glycyrrhizae heat-clearing and toxic substances removing, interior clearing heat in QI system heat, Radix Rhodiolae supplementing QI and nourishing YIN, lung heat clearing blood stasis dispelling, Radix Et Rhizoma Rhei eliminating heat pathogen by purging the bowels, is defend that gas is ruled together, the good medicine of relieving exterior-interior syndrome, heat-clearing and toxic substances removing, shows Drug therapy induced lung injury Be very effective of the present invention by experiment.
The present invention is the improvement invention carried out on the basis of ZL03143211 patent, quotes in full the content of this patent document record at this.The application of above-mentioned patent this Chinese medicine composition unexposed in preparation radiotherapy injury of lung thing.
Summary of the invention
The invention provides a kind of Chinese medicine composition and prepare application in radiotherapy injury of lung medicine, this Chinese medicine composition is made up of the crude drug of following weight portion:
Fructus Forsythiae 200-300 Flos Lonicerae 200-300 Radix Isatidis 200-300 Radix Et Rhizoma Rhei 40-60
Herba Pogostemonis 60-100 Rhizoma Dryopteris Crassirhizomatis 200-300 Radix Rhodiolae 60-100 Mentholum 5-9
Herba Ephedrae (processed) 60-100 Semen Armeniacae Amarum (parched) 60-100 Herba Houttuyniae 200-300
Radix Glycyrrhizae 60-100 Gypsum Fibrosum 200-300.
The weight part ratio of the crude drug of Chinese medicine composition of the present invention is preferably:
Fructus Forsythiae 200 Flos Lonicerae 300 Radix Isatidis 200 Radix Et Rhizoma Rhei 60 Herba Pogostemonis 60
Rhizoma Dryopteris Crassirhizomatis 300 Radix Rhodiolae 60 Mentholum 9 Herba Ephedrae (processed) 60
Semen Armeniacae Amarum (parched) 100 Herba Houttuyniae 200 Radix Glycyrrhizae 100 Gypsum Fibrosum 200.
The weight part ratio of the crude drug of Chinese medicine composition of the present invention is also preferably:
Fructus Forsythiae 300 Flos Lonicerae 200 Radix Isatidis 300 Radix Et Rhizoma Rhei 60 Herba Pogostemonis 100
Rhizoma Dryopteris Crassirhizomatis 200 Radix Rhodiolae 60 Mentholum 5 Herba Ephedrae (processed) 100
Semen Armeniacae Amarum (parched) 60 Herba Houttuyniae 300 Radix Glycyrrhizae 60 Gypsum Fibrosum 300.
The weight part ratio of the crude drug of Chinese medicine composition of the present invention is also preferably:
Fructus Forsythiae 278 Flos Lonicerae 294 Radix Isatidis 285 Radix Et Rhizoma Rhei 55 Herba Pogostemonis 95
Rhizoma Dryopteris Crassirhizomatis 290 Radix Rhodiolae 87 Mentholum 8.5 Herba Ephedrae (processed) 88
Semen Armeniacae Amarum (parched) 80 Herba Houttuyniae 284 Radix Glycyrrhizae 95 Gypsum Fibrosum 277.
The weight part ratio of the crude drug of Chinese medicine composition of the present invention is also preferably:
Fructus Forsythiae 255 Flos Lonicerae 255 Radix Isatidis 255 Radix Et Rhizoma Rhei 51 Herba Pogostemonis 85
Rhizoma Dryopteris Crassirhizomatis 255 Radix Rhodiolae 85 Mentholum 7.5 Herba Ephedrae (processed) 85
Semen Armeniacae Amarum (parched) 85 Herba Houttuyniae 255 Radix Glycyrrhizae 85 Gypsum Fibrosum 255.
Chinese medicine composition of the present invention is primarily of compositions such as Fructus Forsythiae, Flos Lonicerae, Radix Isatidis, Radix Et Rhizoma Rhei, Herba Pogostemonis, Rhizoma Dryopteris Crassirhizomatis, Radix Rhodiolaes, play the integrally-regulated advantage of herbal mixture, the combination of dispeling disease and evil, relief of symptoms, immunity moderation, clinical experiment confirms there is significant curative effect to induced lung injury.
Chinese medicine of the present invention can be had the Chinese medicine of same or similar effect fruit to replace, and these medical materials all can be concocted according to " national Chinese medicine processing specification " or " Chinese medicine voluminous dictionary ".
The active component of Chinese medicine composition of the present invention is made up of following steps:
(1) take Chinese crude drug according to crude drug part by weight, clean;
(2) Herba Pogostemonis is cataclasm, adds 5-8 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;
(3) Fructus Forsythiae, Herba Ephedrae (processed), Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 6-10 times amount 50-90%, each 1-3 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;
(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adds 7-11 times amount soak by water to boiling, and adds Semen Armeniacae Amarum (parched), decocts 2 times, each 0.5-2.5 hour, extracting solution merges filtration, and the filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, and being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.10-1.15, add ethanol, being adjusted to determining alcohol is 70%, and cold preservation is placed, and filters, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;
(5) step (4) gained clear paste and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, and dry, get dry extract powder, for subsequent use;
Step (5) gained dried cream powder, step (2) gained volatile oil and Mentholum form the active component of this Chinese medicine composition jointly.
The dosage form of medicine of the present invention is capsule, tablet, powder, granule, oral liquid, soft capsule, pill, tincture, syrup, suppository, gel, spray or injection.
For enabling above-mentioned dosage form realize, the acceptable adjuvant of pharmacy need be added when preparing these dosage forms, such as: filler, disintegrating agent, lubricant, suspending agent, binding agent, sweeting agent, correctives, antiseptic, substrate etc.Filler comprises: starch, pregelatinized Starch, lactose, mannitol, chitin, microcrystalline Cellulose, sucrose etc.; Disintegrating agent comprises: starch, pregelatinized Starch, microcrystalline Cellulose, carboxymethyl starch sodium, crospolyvinylpyrrolidone, low-substituted hydroxypropyl cellulose, cross-linking sodium carboxymethyl cellulose etc.; Lubricant comprises: magnesium stearate, sodium lauryl sulphate, Pulvis Talci, silicon dioxide etc.; Suspending agent comprises: polyvinylpyrrolidone, microcrystalline Cellulose, sucrose, agar, hydroxypropyl emthylcellulose etc.; Binding agent comprises, starch slurry, polyvinylpyrrolidone, hydroxypropyl emthylcellulose etc.; Sweeting agent comprises: saccharin sodium, Aspartane, sucrose, cyclamate, enoxolone etc.; Correctives comprises: sweeting agent and various essence; Antiseptic comprises: parabens, benzoic acid, sodium benzoate, sorbic acid and its esters, benzalkonium bromide, acetic acid chloroethene are fixed, Folium eucalypti globueli (Eucalyptus globulus Labill.) wet goods; Substrate comprises: PEG6000, PEG4000, insect wax etc.
Wherein capsule is made up of following steps:
(1) take Chinese crude drug according to crude drug part by weight, clean;
(2) Herba Pogostemonis is cataclasm, adds 5-8 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;
(3) Fructus Forsythiae, Herba Ephedrae (processed), Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 6-10 times amount 50-90%, each 1-3 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;
(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adds 7-11 times amount soak by water to boiling, and adds Semen Armeniacae Amarum (parched), decocts 2 times, each 0.5-2.5 hour, extracting solution merges filtration, and the filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, and being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.10-1.15, add ethanol, being adjusted to determining alcohol is 70%, and cold preservation is placed, and filters, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;
(5) step (4) gained clear paste and step (3) gained alcohol extract merge, and being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, and dry, get dry extract powder, for subsequent use;
(6) step (5) gained dried cream powder is added suitable pharmaceutically acceptable adjuvant to granulate;
(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, incapsulates, to obtain final product.
The wherein preparation method of granule is made up of following steps:
(1) take Chinese crude drug according to crude drug part by weight, clean, cataclasm as one sees fit;
(2) Herba Pogostemonis is cataclasm, adds 5-8 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;
(3) Fructus Forsythiae, Herba Ephedrae (processed), Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 6-10 times amount 50-90%, each 1-3 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;
(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adding 7-11 times amount soak by water to boiling, adding Semen Armeniacae Amarum (parched), decoct 2 times, each 0.5-2.5 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, be condensed into 60 DEG C time measure relative density be the clear paste of 1.10-1.15, add ethanol, reconcile to determining alcohol be 70%, cold preservation is placed, filter, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;
(5) step (4) gained gained clear paste and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, and dry, get dry extract powder, for subsequent use;
(6) step (5) gained dried cream powder is added suitable pharmaceutically acceptable adjuvant to granulate;
(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, pack, to obtain final product.
The preparation method of preferred granule is:
(1) take Chinese crude drug according to crude drug part by weight, clean, cataclasm as one sees fit;
(2) Herba Pogostemonis is cataclasm, adds 6 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;
(3) Fructus Forsythiae, Herba Ephedrae (processed), Herba Houttuyniae, Radix Et Rhizoma Rhei, by the ethanol extraction 2 times of 8 times amount 70%, 2 hours first times, second time 1.5 hours, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;
(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adds 9 times amount soak by water to boiling, and adds Semen Armeniacae Amarum (parched), decocts 2 times, 1.5 hours first times, second time 1 hour, extracting solution merges filtration, filter liquor after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.10-1.15, and add 95% ethanol, being adjusted to determining alcohol is 70%, cold preservation is placed, filter, reclaim ethanol to without alcohol taste, obtain clear paste filtrate;
(5) step (4) gained clear paste filtrate and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the thick paste of 1.25-1.35, for subsequent use;
(6) step (5) gained thick paste is added suitable pharmaceutically acceptable adjuvant to granulate;
(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, pack, to obtain final product.
The preparation method of other dosage forms of medicine of the present invention is: weighting raw materials in proportion, adopt conventional preparation method preparation, such as, the preparation technology that Fan Biting " pharmacy of Chinese materia medica " (Shanghai Science Press 1997 December the 1st edition) records, makes the acceptable regular dosage form of pharmaceutics.
Chinese medicine composition of the present invention also can reduce interleukin-6 that induced lung injury causes and or the expression of tumor necrosis factor-alpha and protein level.
accompanying drawing illustrates:
Fig. 1: the impact (HE dyeing × 200) of different time induced lung injury lung tissue of rats pathological change, C. is blank group; R is simple irradiation group; LHQWH is that irradiation adds Chinese medicine composition high dose group of the present invention; LHQWM is that irradiation adds dosage group in Chinese medicine composition of the present invention.
Fig. 2: each time point after irradiating, IL-6 and the TNF-α DNA band of each experimental group, C. is blank group; R is simple irradiation group; LHQWH is that irradiation adds Chinese medicine composition high dose group of the present invention; LHQWM is that irradiation adds dosage group in Chinese medicine composition of the present invention.
Detailed description of the invention
Following embodiment is for illustrating the preparation of medicine of the present invention, but it can not form any restriction to scope of the present invention.
embodiment 1
prescription:
Fructus Forsythiae 255 grams of Flos Loniceraes 255 grams of Radix Isatidis 255 grams of Radix Et Rhizoma Rhei 51 grams
Herba Pogostemonis 85 grams of Rhizoma Dryopteris Crassirhizomatiss 255 grams of Radix Rhodiolaes 85 grams of Mentholums 7.5 grams
Herba Ephedrae (processed) 85 grams of Semen Armeniacae Amarum (parched) 85 grams of Herba Houttuyniae 255 grams
255 grams, Radix Glycyrrhizae 85 grams of Gypsum Fibrosum.
Preparation method:
(1) take Chinese crude drug according to above-mentioned prescription, clean;
(2) Herba Pogostemonis is cataclasm, adds 6 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;
(3) Fructus Forsythiae, Herba Ephedrae (processed), Herba Houttuyniae, Radix Et Rhizoma Rhei, by the ethanol extraction 2 times of 8 times amount 70%, 2 hours first times, second time 1.5 hours, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;
(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adds 9 times amount soak by water to boiling, and adds Semen Armeniacae Amarum (parched), decocts 2 times, 1.5 hours first times, second time 1 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.10-1.15, and add ethanol, being adjusted to determining alcohol is 70%, cold preservation places 24 hours, filter, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;
(5) step (4) gained clear paste and step (3) gained alcohol extract merge, and being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, spraying dry, and get dry extract powder, for subsequent use;
(6) step (5) gained dried cream powder is added starch 142 grams, use 85% alcohol granulation;
(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, loads 1000 capsules, to obtain final product.
embodiment 2
prescription:
Fructus Forsythiae 200 grams of Flos Loniceraes 300 grams of Radix Isatidis 200 grams of Radix Et Rhizoma Rhei 60 grams
Herba Pogostemonis 60 grams of Rhizoma Dryopteris Crassirhizomatiss 300 grams of Radix Rhodiolaes 60 grams of Mentholums 9 grams
Herba Ephedrae (processed) 60 grams of Semen Armeniacae Amarum (parched) 100 grams of Herba Houttuyniae 200 grams
200 grams, Radix Glycyrrhizae 100 grams of Gypsum Fibrosum.
Preparation method:
(1) take Chinese crude drug according to above-mentioned prescription, clean;
(2) Herba Pogostemonis is cataclasm, adds 5 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;
(3) Fructus Forsythiae, Herba Ephedrae (processed), Herba Houttuyniae, Radix Et Rhizoma Rhei, by the ethanol extraction 2 times of 6 times amount 50%, 1 hour first time, second time 2.5 hours, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;
(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adds 7 times amount soak by water to boiling, and adds Semen Armeniacae Amarum (parched), decocts 2 times, 1.5 hours first times, second time 1 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.10-1.15, and add ethanol, being adjusted to determining alcohol is 70%, cold preservation places 24 hours, filter, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;
(5) step (4) gained clear paste and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, spraying dry, and get dry extract powder, for subsequent use;
(6) step (5) gained dried cream powder is added starch 151 grams, use 85% alcohol granulation;
(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, tabletted, obtains 935.
embodiment 3
prescription:
Fructus Forsythiae 300 grams of Flos Loniceraes 200 grams of Radix Isatidis 300 grams of Radix Et Rhizoma Rhei 40 grams
Herba Pogostemonis 100 grams of Rhizoma Dryopteris Crassirhizomatiss 200 grams of Radix Rhodiolaes 100 grams of Mentholums 5 grams
Herba Ephedrae (processed) 100 grams of Semen Armeniacae Amarum (parched) 60 grams of Herba Houttuyniae 300 grams
300 grams, Radix Glycyrrhizae 60 grams of Gypsum Fibrosum.
Preparation method:
(1) take Chinese crude drug according to above-mentioned prescription, clean;
(2) Herba Pogostemonis is cataclasm, adds 8 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;
(3) Fructus Forsythiae, Herba Ephedrae (processed), Herba Houttuyniae, Radix Et Rhizoma Rhei, by the ethanol extraction 2 times of 10 times amount 90%, 3 hours first times, second time 2.5 hours, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;
(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adds 11 times amount soak by water to boiling, and adds Semen Armeniacae Amarum (parched), decocts 2 times, 1.5 hours first times, second time 1 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.10-1.15, and add ethanol, being adjusted to determining alcohol is 70%, cold preservation places 24 hours, filter, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;
(5) step (4) gained clear paste and step (3) gained alcohol extract merge, and being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, spraying dry, and get dry extract powder, for subsequent use;
(6) by step (5) gained dried cream powder, step (2) gained volatile oil and Mentholum make pill according to a conventional method, obtain pill 905 ball.
embodiment 4:
crude drug formula is:
Fructus Forsythiae 170 grams of Flos Loniceraes 170 grams of Herba Ephedrae (processed) 57 grams of Semen Armeniacae Amarum (parched) 57 grams
Gypsum Fibrosum 170 grams of Radix Isatidis 170 grams of Rhizoma Dryopteris Crassirhizomatiss 170 grams of Herba Houttuyniae 170 grams
Herba Pogostemonis 57 grams of Radix Et Rhizoma Rhei 34 grams of Radix Rhodiolaes 57 grams of Mentholums 5.0 grams
57 grams, Radix Glycyrrhizae
preparation method:
(1) extraction process:
(1) take Chinese crude drug according to above-mentioned recipe quantity, clean, cataclasm as one sees fit;
(2) Herba Pogostemonis adds 6 times amount water extraction volatile oil, and carry oil time 4h, collect volatile oil, oil yield is 0.33 ± 0.05%, and for subsequent use after extracting liquid filtering, residue discards;
(3) Fructus Forsythiae, Herba Ephedrae (processed), Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 8 times amount 70%, 2 hours first times, second time 1.5 hours, extracting liquid filtering, filtrate merges, and reclaims ethanol to without alcohol taste, for subsequent use;
(4) Flos Lonicerae, Semen Armeniacae Amarum (parched), Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, add 9 times amount soak by water to boiling, add Semen Armeniacae Amarum (parched), decoct 2 times, 1.5 hours first times, second time 1 hour, extracting liquid filtering, filtrate merge add simultaneously step (2) Herba Pogostemonis carry oil after aqueous solution, being condensed into 60 DEG C of mensuration relative densities is 1.10-1.15 clear paste, add 95% ethanol, limit edged stirs, to determining alcohol 70%, cold preservation places 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, merge with alcohol extract, being concentrated into and being condensed into 60 DEG C of mensuration relative densities is 1.25-1.35 thick paste, for subsequent use,
(2) preparation process:
(5) pharmaceutical formulation is: step (4) gained thick paste 335.5g Mentholum 5g
Step (2) gained patchouli oil 0.2ml Icing Sugar 342.5g dextrin 514.0g
(6) granulate: by Icing Sugar and dextrin mix homogeneously, make binding agent soft material with thick paste, 14 eye mesh screens are granulated, 60-65 DEG C of oven dry, 10 eye mesh screen granulate;
(7) subpackage: sift out fine powder appropriate, Mentholum, Herba Pogostemonis Volatile oil are added ethanol in proper amount, dissolves, sprays in fine powder, mix homogeneously, and mix homogeneously with granule, airtight half an hour, and pack, obtaining final product, above prescription can be made into granule 1000g.
experimental example:
For proved invention Chinese medicine composition has the curative effect of radiotherapy injury of lung, with the capsule (hereinafter referred to as medicine of the present invention) obtained by embodiment 1 method, carry out following pharmacological testing research:
data and method
one, materials and methods
1. laboratory animal and grouping: healthy, grow up, SPF level, female, Wistar rat 48, body weight 180 ~ 200g, purchased from Animal Experimental Study center, Hubei Province (quality certification number: SCXK(Hubei Province) 2008-0005), raise 7 days in clean animal room before experiment, observe person without exception and enter group.Irradiation is divided into by rat to add medicine high dose group (treatedbyhigh-doseLHQWwithradiation of the present invention at random, LHQWH group), irradiate add dosage group (treatedbymiddle-doseLHQWwithradiation in medicine of the present invention, LHQWM group), (radiationonlyGroup of irradiation group merely, R group) and blank group (BlankControlGroup, C group), often organize each 12, and use picric acid labelling.
2. main agents and medicine: Chinese medicine composition of the present invention provides (product batch number: 130232) by Shijiazhuang Yiling Pharmaceutical Co., Ltd; Commercial reagent: Trizol (invitrogen company, the U.S.), MMLV reverse transcriptase and dNTPs(TOYOBO company, Japan), RnaseA enzyme inhibitor (Takara company, Dalian), archaeal dna polymerase (TransGen company, Beijing), IL-6 and TNF-α ELISA detection kit (PeproTech company, the U.S.); OligodT, β-actin, IL-6 and TNF-α primer are synthesized by Shanghai Sheng Gong biological engineering limited company.
3. illuminating method: rat with after chloral hydrate normal saline (350mgkg-1) the row intraperitoneal injection of anesthesia of 10%, dorsal position, extremity launch be fixed on self-control fixing head on, abundant exposed chest, irradiation field size is 2.8cm × 4.2cm, accumulated dose DT=20Gy, SSD=100.0cm.Observe exposure rat by monitor, allly in light period process, occur twisting person, eliminate group.
4. animal process: pre-irradiation starts gavage in 3 days, until after irradiating the 28th day terminates, totally 31 days.LHQWH group: gavage Chinese medicine composition medicated powder of the present invention 0.84gkg-
1body weight, every day 1 time; LHQWM group: gavage Chinese medicine composition medicated powder of the present invention 0.42gkg
-1body weight, every day 1 time; C group and R group: gavage normal saline, every day 1 time.
5. section and HE dyeing: after lung tissue fixes 24h in the formaldehyde fixative of 10%, routine paraffin wax embedding, section, bonding die, dewaxing, HE dyeing, dehydration, transparent, mounting, by optical microscope (OLYMPUSIX81, Japan) row sections observation and image acquisition.
6. bronchoalveolar lavage fluid cell counting: post mortem thoracic cavity, rat place, ligation left principal bronchus, with PBS lavation right lung after tracheal intubation, each 2mL, totally 3 times, the response rate is about 90%.By bronchoalveolar lavage fluid at 4 DEG C
With the centrifugal 5min of 1200r/min, abandon supernatant, repeat to wash 2 times, finally count with 4mlPBS re-suspended cell.
7. in serum, interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) measure: after rat anesthesia, heart extracting blood is about 4mL, 3000r/min, centrifugal 10min, gets serum in-80 DEG C of preservations.In serum specimen, the detection of IL-6 and TNF-α operates according to ELISA kit description.
8.RT-PCR method detects the expression of lung tissue IL-6 and TNF-α mRNA: extract 50mg lung tissue total serum IgE with 1mlTrizol, RNA purity (A260/A280=1.6 ~ 1.8) and concentration is measured with ultraviolet spectrophotometer, get the capable reverse transcription reaction of 0.5 μ g total serum IgE by specification, 30 DEG C of reaction 10min, 42 DEG C of reaction 60min, 70 DEG C of reaction 15min.Get 1 μ lRT product performing PCR, PCR reaction presses 94 DEG C of denaturation 3.5min, 94 DEG C of 30s, 53 DEG C of 15s, 72 DEG C of 20s, 33 circulations.Primer sequence is as table 1.Pcr amplification product is taken pictures observed result after 1% agarose gel electrophoresis.
9. statistical analysis
Use SPSS17.0 statistical software analyze, experimental data all with mean ± standard deviation (
x± S) represent, compare t inspection and variance analysis between employing group between group, represent that difference has statistical significance with P<0.05, P<0.01 represents that difference has statistical significance.
Two, experimental result
1. rat ordinary circumstance
Simple irradiation group rat occurs on the 3rd day that after irradiating lethargy, food-intake and activity reduce, yellow secretions appears in eyes, large dry stool, within 14th day, irradiate local afterwards and occur depilation, there is cough symptom in partial rat, after irradiating, the 26th day above-mentioned symptom starts to alleviate gradually.And irradiation adds medicine of the present invention high, middle dosage group rat mental status irradiation group more merely well, the incidence rate of eye discharge, the aspect such as stiff of defecating is low, degree is comparatively light, time of occurrence is more late, the persistent period is shorter, to irradiating latter 18th day, above-mentioned performance starts to fade away, without symptom of obviously coughing.
2. pathological tissue is observed
Irradiation adds high, the middle dosage group of the medicine of the present invention 1st day a small amount of inflammatory cell infiltration of rarely seen interstitial lung after irradiation, within 14th day, see that interstitial lung gently arrives the acute inflammation such as intermediate edema and inflammatory cell infiltration and changes, when 28 days, inflammation obviously alleviates, and interstitial lung thickens, alveolar structure slight damage.And namely simple irradiation group is shown in that interstitial lung thickens on the 1st day after irradiating, inflammatory cell infiltration, when the 14th day, inflammatory reaction is serious, show as interstitial lung hyperemia, hemorrhage, edema, massive inflammatory cells infiltrated, alveolar structure destroys, and when the 28th day, inflammation slightly alleviates, interstitial lung thickens, and alveolar space diminishes (as Fig. 1).
3. bronchoalveolar lavage fluid cell counting: After Irradiation In Rats bronchoalveolar lavage fluid total cellular score obviously raises, compared with simple irradiation group, medicine high dose group of the present invention each time point bronchoalveolar lavage fluid total cellular score after irradiation all lowers (as table 2), wherein the 14th, 28 day difference has statistical significance (t=3.237,3.431, P<0.05) significantly.In medicine of the present invention, the bronchoalveolar lavage fluid total cellular score of dosage group lowers more to some extent with simple irradiation group, but the 14th day no significant difference (t=1.710 after irradiating, P>0.05), and postradiation 28th day both comparing differences significantly (t=2.658, P<0.05).
Radioactivity induced lung injury in rats bronchoalveolar lavage fluid cell counting (x ± S, × 104/ml) under table 2. Chinese medicine composition effect of the present invention
Note: LHQWH group compares with R group: t=3.237, #P<0.05; T=3.431, ##P<0.05.
LHQWM group compares with R group: t=1.710, ###P>0.05; T=2.658, ####P<0.05.
4. interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) level determination in serum
In acute radiation pneumonitis rat model serum, IL-6 and TNF-a level all obviously raises, but after using medicine of the present invention, IL-6 and TNF-a level all reduces (as table 3).Wherein, in IL-6 level, the inhibitory action of high dose medicine of the present invention is more obvious, compares the 1st, 14,28 day difference after irradiating all have statistical significance (t=7.748,15.119,6.902, P<0.01) with simple irradiation group; And in TNF-a level, the inhibitory action of middle dosage medicine of the present invention is more obvious, with simple irradiation group the 1st day difference more after irradiation, all there is statistical significance (t=3.940, P<0.01), after irradiating, the 14th, 28 day difference also has statistical significance (t=3.333,2.259, P<0.05).
Radioactivity induced lung injury in rats serum TNF-aandIL-6 level (x ± S, ng/ml) under table 3. Chinese medicine composition effect of the present invention
Note: LHQWH group compares with R group: t=3.940, IV P<0.01; T=3.333, t=2.259, V P<0.05.LHQWM group compares with R group: t=7.748, t=15.119, t=6.902, III P<0.01.
5. the expression of lung tissue interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) mRNA
Each time point, IL-6 with the TNF-α DNA band of each experimental group and the relative gray values (see Fig. 2, table 4) of relative β-actin internal reference band after irradiating.Compare with simple irradiation group, irradiate to add to connect and spend the relative gray values of IL-6 and the TNF-α band of high, the middle dosage group of pestilence clearly all to reduce, difference has statistical significance (P<0.01).
The impact that table 4. Chinese medicine composition of the present invention is expressed TNF-α and IL-6mRNA in induced lung injury lung tissue of rats
C. blank group; LHQWH. irradiate and add Chinese medicine composition high dose group of the present invention; LHQWM. irradiate and add dosage group in Chinese medicine composition of the present invention; R. irradiation group merely
three, conclusion
Can show that Chinese medicine composition of the present invention can improve the ordinary circumstance of rat by testing above, alleviate the inflammatory reaction of lung tissue, suppress the rising of the total cellular score in the bronchoalveolar lavage fluid caused by acute radiation pneumonitis, thus play the effect of control induced lung injury.Chinese medicine composition of the present invention suppresses rat blood serum IL-6 and TNF-α protein level and lowers the expression damaging IL-6 and TNF-α mRNA in lung tissue.
Experimental result abundant proved invention medicine can effective radiotherapy injury of lung, be specially adapted to interleukin-6 (IL-6) that induced lung injury causes and tumor necrosis factor-alpha (TNF-α) raises, there is not any clinical adverse and toxic and side effects in treatment overall process, there is significant clinical efficacy.
Claims (10)
1. the application of Chinese medicine composition in preparation radiotherapy injury of lung medicine, is characterized in that described Chinese medicine composition is made up of the crude drug of following weight portion:
Fructus Forsythiae 200-300 Flos Lonicerae 200-300 Radix Isatidis 200-300 Radix Et Rhizoma Rhei 40-60
Herba Pogostemonis 60-100 Rhizoma Dryopteris Crassirhizomatis 200-300 Radix Rhodiolae 60-100 Mentholum 5-9
Herba Ephedrae (processed) 60-100 Semen Armeniacae Amarum (parched) 60-100 Herba Houttuyniae 200-300
Radix Glycyrrhizae 60-100 Gypsum Fibrosum 200-300.
2. application according to claim 1, is characterized in that being made up of the crude drug of following weight portion:
Fructus Forsythiae 200 Flos Lonicerae 300 Radix Isatidis 200 Radix Et Rhizoma Rhei 60 Herba Pogostemonis 60
Rhizoma Dryopteris Crassirhizomatis 300 Radix Rhodiolae 60 Mentholum 9 Herba Ephedrae (processed) 60
Semen Armeniacae Amarum (parched) 100 Herba Houttuyniae 200 Radix Glycyrrhizae 100 Gypsum Fibrosum 200.
3. application according to claim 1, is characterized in that being made up of the crude drug of following weight portion:
Fructus Forsythiae 300 Flos Lonicerae 200 Radix Isatidis 300 Radix Et Rhizoma Rhei 40 Herba Pogostemonis 100
Rhizoma Dryopteris Crassirhizomatis 200 Radix Rhodiolae 100 Mentholum 5 Herba Ephedrae (processed) 100
Semen Armeniacae Amarum (parched) 60 Herba Houttuyniae 300 Radix Glycyrrhizae 60 Gypsum Fibrosum 300.
4. application according to claim 1, is characterized in that being made up of the crude drug of following weight portion:
Fructus Forsythiae 278 Flos Lonicerae 294 Radix Isatidis 285 Radix Et Rhizoma Rhei 55 Herba Pogostemonis 95
Rhizoma Dryopteris Crassirhizomatis 290 Radix Rhodiolae 87 Mentholum 8.5 Herba Ephedrae (processed) 88
Semen Armeniacae Amarum (parched) 80 Herba Houttuyniae 284 Radix Glycyrrhizae 95 Gypsum Fibrosum 277.
5. application according to claim 1, is characterized in that being made up of the crude drug of following weight portion:
Fructus Forsythiae 255 Flos Lonicerae 255 Radix Isatidis 255 Radix Et Rhizoma Rhei 51 Herba Pogostemonis 85
Rhizoma Dryopteris Crassirhizomatis 255 Radix Rhodiolae 85 Mentholum 7.5 Herba Ephedrae (processed) 85
Semen Armeniacae Amarum (parched) 85 Herba Houttuyniae 255 Radix Glycyrrhizae 85 Gypsum Fibrosum 255.
6. the application according to any one of claim 1-5, is characterized in that the active component of described medicine is made up of following steps:
(1) take Chinese crude drug according to crude drug part by weight, clean;
(2) Herba Pogostemonis is cataclasm, adds 5-8 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;
(3) Fructus Forsythiae, Herba Ephedrae (processed), Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 6-10 times amount 50-90%, each 1-3 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;
(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adds 7-11 times amount soak by water to boiling, and adds Semen Armeniacae Amarum (parched), decocts 2 times, each 0.5-2.5 hour, extracting solution merges filtration, and the filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, and being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.10-1.15, add ethanol, being adjusted to determining alcohol is 70%, and cold preservation is placed, and filters, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;
(5) step (4) gained clear paste and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, and dry, get dry extract powder, for subsequent use;
Step (5) gained dried cream powder, step (2) gained volatile oil and Mentholum form the active component of this Chinese medicine composition jointly.
7. the application according to any one of claim 1-5, is characterized in that described pharmaceutical dosage form is capsule, tablet, powder, granule, oral liquid, pill, tincture, syrup, suppository, gel, spray or injection.
8. application according to claim 7, is characterized in that described capsule is made up of following steps:
(1) take Chinese crude drug according to crude drug part by weight, clean;
(2) Herba Pogostemonis is cataclasm, adds 5-8 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;
(3) Fructus Forsythiae, Herba Ephedrae (processed), Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 6-10 times amount 50-90%, each 1-3 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;
(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adds 7-11 times amount soak by water to boiling, and adds Semen Armeniacae Amarum (parched), decocts 2 times, each 0.5-2.5 hour, extracting solution merges filtration, and the filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, and being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.10-1.15, add ethanol, being adjusted to determining alcohol is 70%, and cold preservation is placed, and filters, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;
(5) step (4) gained clear paste and step (3) gained alcohol extract merge, and being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, and dry, get dry extract powder, for subsequent use;
(6) step (5) gained dried cream powder is added suitable pharmaceutically acceptable adjuvant to granulate;
(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, incapsulates, to obtain final product.
9. application according to claim 7, is characterized in that described granule is made up of following steps:
(1) take Chinese crude drug according to crude drug part by weight, clean, cataclasm as one sees fit;
(2) Herba Pogostemonis is cataclasm, adds 5-8 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;
(3) Fructus Forsythiae, Herba Ephedrae (processed), Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 6-10 times amount 50-90%, each 1-3 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;
(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adding 7-11 times amount soak by water to boiling, adding Semen Armeniacae Amarum (parched), decoct 2 times, each 0.5-2.5 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, be condensed into 60 DEG C time measure relative density be the clear paste of 1.10-1.15, add ethanol, reconcile to determining alcohol be 70%, cold preservation is placed, filter, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;
(5) step (4) gained gained clear paste and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, and dry, get dry extract powder, for subsequent use;
(6) step (5) gained dried cream powder is added suitable pharmaceutically acceptable adjuvant to granulate;
(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, pack, to obtain final product.
10. the application according to any one of claim 1-5, is characterized in that the interleukin-6 that preparation radiotherapy injury of lung causes and/or tumor necrosis factor-alpha raise.
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| EP3705130A4 (en) * | 2017-11-10 | 2021-07-28 | Shijiazhuang Yiling Pharmaceutical Co., Ltd. | Method for separating eight components in chinese traditional medicine composition |
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