CN105076368A - Bamboo leaf extract used as fruit and vegetable fresh-keeping agent - Google Patents

Abstract

The invention discloses a bamboo leaf extract used as fruit and vegetable fresh-keeping agent, which is obtained by the following steps: 1)taking the bamboo leaf and drying, using 5-15 times amount of ethanol solution with concentration being 50-100% for immersing, and performing reflux extraction for several times, wherein each extraction time is 1-5 hours, filtrating to obtain an ethanol extract and residues, merging the ethanol extract, standing for 12-48 hours, filtrating the sediment, and recovering the solvent to obtain a crude extract; 2)dissolving the crude extract by water, loading on macroporous absorbent resin columns, eluting and desugaring, using the ethanol solution with concentration being 50-100% and eluting, recovering to obtain the extract; 3)dissolving the extract by water, using 10-20 times amount of dichloromethane, ethyl acetate and n-butyl alcohol for extracting; recovering the solvent to obtain a dichloromethane extract, an ethyl acetate extract and a n-butyl alcohol extract; merging the dichloromethane extract, the ethyl acetate extract and the n-butyl alcohol extract to obtain a total extract. The bamboo leaf extract can effectively prolong the refreshing time of fruit and vegetables, keep fruit hardness, and delay decreasing rate of chlorophyll.

Description

A kind of bamboo extractive that can be used as fruit and vegetable fresh-keeping agent

Technical field

The invention belongs to natural plant extracts technical field, be specifically related to a kind of bamboo extractive that can be used as fruit and vegetable fresh-keeping agent.

Background technology

Bamboo extractive, also known as leaf of bamboo alkali; To shine the leaf of bamboo of dry grinding for raw material, adopt cellulase pretreatment, watery hydrochloric acid lixiviate, ultrasonic assistant to extract, merge extract, pH value is adjusted in alkalization, concentration and evaporation water, to the medicinal extract of proportion D=1.15, cools, and 10 times amount chloroforms stir, leave standstill, layering, aqueous phase continues extraction three times, combined chloroform phase, reclaim chloroform, medicinal extract dusts, and pulverizes, mixing, packaging both obtained.Main chemical compositions has leaf of bamboo alkaloid, bamboo-leaves flavones, citric acid, oxalic acid etc.

Summary of the invention

Goal of the invention: for the deficiencies in the prior art, the object of this invention is to provide a kind of bamboo extractive that can be used as fruit and vegetable fresh-keeping agent, can be used as fruit and vegetable fresh-keeping agent.

Technical scheme: for achieving the above object, the technical solution used in the present invention is as follows:

A kind of bamboo extractive that can be used as fruit and vegetable fresh-keeping agent, it is characterized in that: comprise (1) and get leaf of bamboo material, drying, after soaking with 5 ~ 15 times amount 50 ~ 100% ethanol waters, refluxing extraction is for several times, extracts 1 ~ 5h at every turn, filter, obtain ethanol extract and filter residue, merge ethanol extract, leave standstill 12 ~ 48h, elimination precipitates, and recycling design obtains crude extract; (2) water-soluble crude extract, upper large pore resin absorption column, after washing desugar, with 50 ~ 100% ethanol water wash-outs, collect ethanol eluate, recycling design obtains extract; (3) water-soluble extract, uses 10 ~ 20 times amount carrene, ethyl acetate, extracting n-butyl alcohol successively; Recycling design obtains carrene extractive part, ethyl acetate extract extract, n-butanol portion extract respectively; Combined dichloromethane extractive part, ethyl acetate extract extract, n-butanol portion extract both obtained total extract.

Prepared bamboo extractive.

The prepared application of bamboo extractive in fruit and vegetable fresh-keeping agent.

Beneficial effect: compared with prior art, bamboo extractive prepared by the present invention, effectively can extend the freshness date of fruits and vegetables, reduces ethylene gas release, keep fruits and vegetables hardness, delay chlorophyllous decrease speed, reduce moisture evaporation, reduce the injury from low temperature of fruits and vegetables, maintain the balance of sugar and acid degree, reduce scratch brown stain and block harmful bacterium and prevent from propagating harm, and prevent mouldy, increase fruits and vegetables gloss.

Detailed description of the invention

Illustrate the present invention further below in conjunction with specific embodiment, this detailed description of the invention is implemented under premised on technical solution of the present invention, should understand these modes and only be not used in for illustration of the present invention and limit the scope of the invention.

Embodiment 1

Bamboo extractive is obtained by following methods:

(1) get leaf of bamboo material, dry, after soaking with 5 ~ 15 times amount 50 ~ 100% ethanol waters, refluxing extraction for several times, extracts 1 ~ 5h at every turn, filters, obtain ethanol extract and filter residue, merges ethanol extract, leaves standstill 12 ~ 48h, and elimination precipitates, and recycling design obtains crude extract; (2) water-soluble crude extract, upper large pore resin absorption column, after washing desugar, with 50 ~ 100% ethanol water wash-outs, collect ethanol eluate, recycling design obtains extract; (3) water-soluble extract, uses 10 ~ 20 times amount carrene, ethyl acetate, extracting n-butyl alcohol successively; Recycling design obtains carrene extractive part, ethyl acetate extract extract, n-butanol portion extract respectively; Combined dichloromethane extractive part, ethyl acetate extract extract, n-butanol portion extract both obtained total extract.

Embodiment 2

Fruit and vegetable fresh-keeping agent, be made up of each component of following mass fraction: 50% citric acid, sodium hydrogensulfite, 10% bamboo extractive, 0.03% phytate, 1% amino acid, 1% Cobastab, 1% nicotinic acid, 1%Vc, 0.1% trace element (iron, copper, zinc, cobalt, manganese, chromium, selenium, iodine, nickel, fluorine, molybdenum, vanadium, tin, silicon, strontium, boron, rubidium, arsenic, Deng heavy), each constituent mass mark sum is 100%.

Above each component is first used water-soluble solution, then dry, pack.During use, first with cold water slightly, by stand-by antistaling agent furnishing pasty state, be advisable to lose dry powder, then dilute in the aseptic clear water of 1.5--2% ratio, in dilution, can stir, while pastel is scraped to container wall with wooden stick, make thinner, antistaling agent and solubilized.

The general method adopting spraying or dipping, both one deck can be formed without the transparent edible film of palladium at fruit and vegetable surfaces after drying, also rear direct vanning can be processed, natural air drying, without the need to professional drying equipment, also can transport after the in-situ processing of the edge of a field rand or preserve, also can be used for the machinery waxing coating problems of fresh-keeping factory.With good conditionsily also can load the nontoxic thick plastic bag moisturizing of 0.02-0.04mm after treatment, pump air in bag with gas bucket, strengthen fresh-keeping effect, long-distance storing of casing.

This antistaling agent, natural air drying is fast, adhesion after film forming, does not dispute over trifles, the resistance to temperature difference, is beneficial to a large amount of fresh products and uses continuously, can not produce resistance, green non-poisonous.

Comparative example

Test material: fresh fruit of vegetables, the commercially available antistaling agent of certain brand, the antistaling agent prepared by above-described embodiment.

Experimental enviroment: temperature: 3-4 DEG C.

Test period: 30 days.

Test site: freezer.

The crisp hardness of the weightless healthy fruit color and luster of project is damaged by worms rate

The present invention 4.1598% pure color better 0

Certain brand 5.6392% shows slightly brown general 0

Blank sample 7.9580% shades, and brown stain seriously obviously feels like jelly 11%.

Claims (2)

1. can be used as a bamboo extractive for fruit and vegetable fresh-keeping agent, it is characterized in that, prepared by following methods:

(1) get leaf of bamboo material, dry, after soaking with 5 ~ 15 times amount 50 ~ 100% ethanol waters, refluxing extraction for several times, extracts 1 ~ 5h at every turn, filters, obtain ethanol extract and filter residue, merges ethanol extract, leaves standstill 12 ~ 48h, and elimination precipitates, and recycling design obtains crude extract; (2) water-soluble crude extract, upper large pore resin absorption column, after washing desugar, with 50 ~ 100% ethanol water wash-outs, collect ethanol eluate, recycling design obtains extract; (3) water-soluble extract, uses 10 ~ 20 times amount carrene, ethyl acetate, extracting n-butyl alcohol successively; Recycling design obtains carrene extractive part, ethyl acetate extract extract, n-butanol portion extract respectively; Combined dichloromethane extractive part, ethyl acetate extract extract, n-butanol portion extract both obtained total extract.

2. the application of bamboo extractive according to claim 1 in fruit and vegetable fresh-keeping agent.