CN103828911A - Fresh-keeping method of bergamot fruits with pine needle extract - Google Patents
Fresh-keeping method of bergamot fruits with pine needle extract Download PDFInfo
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- CN103828911A CN103828911A CN201210490418.6A CN201210490418A CN103828911A CN 103828911 A CN103828911 A CN 103828911A CN 201210490418 A CN201210490418 A CN 201210490418A CN 103828911 A CN103828911 A CN 103828911A
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Abstract
The invention provides a fresh-keeping method of bergamot fruits with a pine needle extract, relates to the preservation and anti-corrosive field of foods and medicines and develops a fresh-keeping product with no chemical harm, no residue, good effects and low cost by using plant resources as raw materials. A product of a pine needle extracting solution with a fresh-keeping function is prepared by combining a traditional fresh-keeping material of a pine needle with a modern extraction process and adopting a proper solvent. The pine needle extracting solution has strong inhibition effects on gram positive bacteria, gram negative bacteria and mycete and strong antioxidant functions. The pine needle extracting solution acts on the bergamot fruit in a manner of smearing and a waterproof preservative film is additionally used, thus the fresh-keeping method has the characteristics of convenience, environment protection, good effects and low cost and can be well applied to fresh keeping of the bergamot fruits.
Description
Technical field
The invention provides the preservation method of a kind of extract from pine needles to bergamot fruit, is that development, without chemical murder by poisoning, noresidue, fresh products effective, with low cost, relates to the preservation and antisepsis field of food and medicine taking plant resources as raw material.
Background technology
Jinhua, Zhejiang is the most famous place of production of Buddha's hand, is called as " the celestial product in fruit, the strange grass of world ", refined title " gold bergamot "." gold bergamot " has high ornamental value, be of moderate size, and form grace, top slight crack is obvious, favored by the personage of China and foreign countries.But " gold bergamot " is just difficult to buy on market after the Spring Festival; Due to " gold bergamot " the fresh-keeping problem in transport and storage process, cause its staple market to be confined to Jinhua Region simultaneously.The real existing preservation method of bergamot fruit has deepfreeze method and chemical substance spraying, preservation by low temperature method due to have drop into high, demand strict technology, energy consuming ratio is larger, do not meet the shortcomings such as the requirement of " energy-saving and emission-reduction ", increasing income with socioeconomic sustainable development of industry development to Jinhua Fingered Citron, peasant is all very unfavorable.And chemical substance spraying is owing to having potential contaminative in sprinkling process, do not meet green environmental requirement.Therefore the fresh-keeping composition that, extracts environmental protection from natural plants has very strong realistic meaning for bergamot fruit fresh-keeping.
Summary of the invention
The present invention combines traditional Perserving materials one pine needle with modern extraction process, adopt suitable solvent extraction and prepare product one pine needle extract with fresh-keeping function.This pine needle extract has stronger inhibitory action to gram positive bacteria, gram-negative bacteria, mould, and has stronger anti-oxidation function.This pine needle extract adopts the mode of smearing to act on Buddha's hand fresh fruit, is aided with the additional waterproof of preservative film, and such preservation method has convenience, environmental protection, the feature that effective, cost is low, can be advantageously applied to the fresh-keeping of bergamot fruit.
1. a kind of extract from pine needles of preparation, is characterized in that:
Adopt fresh needles, after washing, drain, be cut into 1～2cm, add the ethanol of 50% concentration with the solid-liquid ratio of 1:10, refluxing extraction 2.5h, filters, and collects filtrate, extract is concentrated final vacuum freeze drying suitably, and I dissolves extract before use with 50% ethanol, be made into 200mg.mL
-1solution for standby.Particular content is as follows:
(1) water, 95% ethanol, ethyl acetate, benzinum extract pine needle, adopt the relatively bacteriostatic activity of different solvents extract of filter paper method.Result water and 95% ethanol fungistatic effect are better.
(2) with 25%, 50%, 75%, the ethanol of 95% 4 kind of variable concentrations extracts pine needle, adopts the relatively bacteriostatic activity of different concentration ethanol extract of filter paper method.The ethanolic solution fungistatic effect of 50% concentration is better as a result.
(3) by microwave extraction method, super Lu's extraction method, cocurrent flow extraction method and soxhlet extraction method, pine needle is extracted respectively, adopt the relatively bacteriostatic activity of Different Extraction Method of filter paper method.The extract fungistatic effect that result reflux extraction obtains is better.
(4) respectively cdear, masson pine, black pine pine needle are extracted, adopt the flight of steps leading to a palace hall antibacterial alive of the more different extract from pine needles of filter paper method.Result cedar needles press down fierce effect better.
2. the extract from pine needles of application rights requirement 1 is protected a fine method for Buddha's hand, it is characterized in that:
The tangible indoor sweating 24h of bergamot fruit plucking, removes the part moisture of fruit surface, gets pine needle extract and is applied in equably and treats on fresh-keeping Buddha's hand, dry the then clean preservative film of overcoat modest size, preservative film sealing at the place of drying in the shade, under normal temperature laboratory, preserve, avoid direct sunlight.Particular content is as follows:
Bergamot fruit for test (first at indoor sweating 24h, removing the part moisture of fruit surface), be divided into four parts, observe after smearing bergamot fruit with extract for first part, to smear after bergamot fruit with extract for second part, overcoat preservative film is observed.The 3rd part adds after 20% glycerine in extract, observes after spreading upon bergamot fruit.The 4th part is added after 0.1% shitosan in extract, observes after smearing bergamot fruit with extract.Make blank for the 5th part, observe at regular intervals fresh-keeping effect (with evaluations such as freshness, weight-loss ratios).Result is smeared after bergamot fruit with extract, and the method for overcoat preservative film is compared other method, has difference, good refreshing effect.
Detailed description of the invention
1. the pine needle infrastest fresh-keeping to Buddha's hand
Implementation method: control group is established in experiment, and half Buddha's hand fresh fruit is not placed pine needle, is placed directly in chest, and sealing is preserved and observed, and second half lays pine needle at the bottom of chest, puts Buddha's hand fresh fruit, covers pine needle, chest sealing above simultaneously.Control group and experimental group are observed every two weeks, investigate index: decayed fruit rate, weight-loss ratio and freshness.
2. the fungistatic effect of pine needle research
(1) bacteriostatic test of pine needle different solvents extract
Water, 95% ethanol, ethyl acetate, benzinum extract cedar needles respectively, adopt the relatively bacteriostatic activity of different solvents extract of filter paper method.Get common bacterium and fungi and make bacteriostatic experiment, every kind of bacterium respectively water, 95% ethanol, ethyl acetate, ligroin extraction makes bacteriostatic experiment, observation index: inhibition zone size.
(2) bacteriostatic test of pine needle different concentration ethanol extract
Respectively with 25%, 50%, 75%, the ethanol of 95% 4 kind of variable concentrations extracts cedar needles, adopts the relatively bacteriostatic activity of different concentration ethanol extract of filter paper method.Choose common bacterium and fungi and make bacteriostatic experiment, every kind of bacterium makes bacteriostatic experiment, observation index with above-mentioned four kinds of concentration ethanol extracts respectively: inhibition zone size.
(3) impact of pine needle Different Extraction Method on fungistatic effect
By microwave extraction method, ultrasonic extraction, reflux extraction and soxhlet extraction method, cedar needles is extracted respectively, adopt the relatively bacteriostatic activity of Different Extraction Method of filter paper method.Choose common bacterium and fungi and make bacteriostatic experiment, every kind of bacterium makes bacteriostatic experiment, observation index with above-mentioned four kinds of extracts respectively: inhibition zone people is little.
(4) fungistatic effect of different pine needles research
Respectively cdear, masson pine, black pine pine needle are extracted, adopt the bacteriostatic activity of the more different extract from pine needles of filter paper method.Choose common bacterium and fungi thousand bacteriostatic experiment, every kind of bacterium respectively just above-mentioned two kinds of extracts make bacteriostatic experiment, observation index: inhibition zone people is little.
3. pine needle extract acts on the fresh-keeping effect observation of Buddha's hand fresh fruit
To put up a question: extract from pine needles acts on the mode of bergamot fruit, and which kind of is better, and Buddha's hand is fresh-keeping, also needs to add moisturizing composition (glycerine, shitosan) on extract, moisturizing composition is added in experiment comparative study and which is effective with preservative film?
Method: determined by bacteriostatic experiment after the optimum extraction solvent, optimum extracting method of pine needle, prepared pine needle extract for subsequent use.Test bergamot fruit, be divided into four parts, observe after smearing bergamot fruit with extract for first part, to smear after bergamot fruit with extract for second part, overcoat preservative film is observed.The 3rd part adds after 20% glycerine in extract, observes after spreading upon bergamot fruit.The 4th part is added after 0.1% shitosan in extract, observes after smearing bergamot fruit with extract.Make blank for the 5th part, observe at regular intervals fresh-keeping effect (with evaluations such as freshness, weight-loss ratios).
Claims (2)
1. an extract from pine needles, is characterized in that:
Adopt fresh needles, after washing, drain, be cut into 1～2cm, add the ethanol of 50% concentration with the solid-liquid ratio of 1: 10, refluxing extraction 2.5h, filters, and collects filtrate, extract is concentrated final vacuum freeze drying suitably, before use extract is dissolved by 50% second-ol, is made into 200mg.mL
-lsolution for standby.
2. the extract from pine needles of application rights requirement 1, for the fresh-keeping method of Buddha's hand, is characterized in that:
The tangible indoor sweating 24h of bergamot fruit plucking, removes the part moisture of fruit surface, gets pine needle extract and is applied in equably and treats on fresh-keeping Buddha's hand, dry the then clean preservative film of overcoat modest size, preservative film sealing at the place of drying in the shade, under normal temperature laboratory, preserve, avoid direct sunlight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210490418.6A CN103828911A (en) | 2012-11-21 | 2012-11-21 | Fresh-keeping method of bergamot fruits with pine needle extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210490418.6A CN103828911A (en) | 2012-11-21 | 2012-11-21 | Fresh-keeping method of bergamot fruits with pine needle extract |
Publications (1)
| Publication Number | Publication Date |
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| CN103828911A true CN103828911A (en) | 2014-06-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210490418.6A Pending CN103828911A (en) | 2012-11-21 | 2012-11-21 | Fresh-keeping method of bergamot fruits with pine needle extract |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577996A (en) * | 2015-10-14 | 2017-04-26 | 嘉禾县金森农业科技发展有限公司 | Ultra long time fresh-keeping method for Lactarius deliciosus |
| CN108261112A (en) * | 2016-08-21 | 2018-07-10 | 王琪 | A kind of walnut opener |
| CN108294656A (en) * | 2016-08-21 | 2018-07-20 | 王琪 | A kind of nut fruit openers |
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Patent Citations (4)
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| CN1813823A (en) * | 2005-02-05 | 2006-08-09 | 湖南韶山元生科技有限公司 | Method for extracting flavone from pine needle |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577996A (en) * | 2015-10-14 | 2017-04-26 | 嘉禾县金森农业科技发展有限公司 | Ultra long time fresh-keeping method for Lactarius deliciosus |
| CN108261112A (en) * | 2016-08-21 | 2018-07-10 | 王琪 | A kind of walnut opener |
| CN108294656A (en) * | 2016-08-21 | 2018-07-20 | 王琪 | A kind of nut fruit openers |
| CN108378731A (en) * | 2016-08-21 | 2018-08-10 | 王琪 | A kind of walnut opener with preservation |
| CN108451406A (en) * | 2016-08-21 | 2018-08-28 | 王琪 | Nut fruit openers |
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