CN105055857A - Traditional Chinese medicine preparation for treating stress diseases of Chinese softshell turtles and preparation method of preparation - Google Patents
Traditional Chinese medicine preparation for treating stress diseases of Chinese softshell turtles and preparation method of preparation Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine preparation for treating stress diseases of Chinese softshell turtles and a preparation method of the preparation. The traditional Chinese medicine preparation is powder prepared from the following medicinal materials in percentage by weight: 5-11% of ligusticum chuanxiong hort, 5-11% of salvia miltiorrhiza, 2-8% of angelicae sinensis, 2-8% of leonurus, 7-13% of uncaria rhynchophylla, 7-13% of rhizoma corydalis, 5-11% of jujube seed, 5-11% of radix bupleuri, 5-11% of polygonatum sibiricum, 5-11% of astragalus membranaceus, 5-11% of epimedium, 5-11% of schisandra chinensis, 2-8% of radix glycyrrhizae, and 0.5-1.5% of vitamin C; the total percentage of all components is 100%. The traditional Chinese medicine preparation for treating stress diseases of Chinese softshell turtles, provided by the invention, has the characteristics of being good in curative effect on treating the white spot disease of the Chinese softshell turtles and free of drug residues.
Description
Technical field
The present invention relates to a kind of Chinese medicine preparation, more particularly, particularly relate to the Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease, the invention still further relates to the preparation method of this kind of Chinese medicine preparation.
Background technology
In Trionyx sinensis (Wiegmann) breeding process, when Trionyx sinensis (Wiegmann) sense organ to extraneous there is certain environment change time, Trionyx sinensis (Wiegmann) body stimulates to external world can produce corresponding specific variations, and this change is that Trionyx sinensis (Wiegmann) attempts to adapt to the change of internal and external environment and the non-specific change that produces.The Trionyx sinensis (Wiegmann) stress disease that so-called " environment-stress " factor causes is exactly the nonspecific reaction that Trionyx sinensis (Wiegmann) is subject to the stimulation generation of external environment condition change.Usual Trionyx sinensis (Wiegmann) stress disease shows as: under the working condition of intensive culture, especially during Raised turtle as there is the ecological ragime of high temperature, high density, high pollution, or when Trionyx sinensis (Wiegmann) Trionyx sinensis Wiegmann is faced with far beyond its stressors at aspects such as the contingent environment in natural life area, physiology and immunity, easily produce stress disease.As under the condition of high temperature, high pollution and severe depletion of oxygen, Trionyx sinensis (Wiegmann) can be had dizzy spells, stress is strong, often shows as and emerges, head rocks on the water surface, is far from being tranquil, and the time one is long, will produce neurotoxic, occur that forelimb bends the symptom that can not stretch, mortality rate is more than 20%.Stress disease makes the fertility performance of Trionyx sinensis (Wiegmann) decline, minimizing of ingesting, and produces larger adverse influence to feed conversion rate, the speed of growth, survival rate etc.Mainly take vitamin C during traditional treatment Trionyx sinensis (Wiegmann) stress disease, and it is undesirable singly to use this kind of medicine to carry out therapeutic effect.Therefore, how to find a kind of pharmaceutical preparation to treat Trionyx sinensis (Wiegmann) stress disease, make its therapeutic effect better, and drug residue free, become problem demanding prompt solution.
Summary of the invention
The object of the present invention is to provide the Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease, this kind of Chinese medicine preparation has to treatment Trionyx sinensis (Wiegmann) stress disease that therapeutic effect is good, the feature of drug residue free.
Another object of the present invention is to provide a kind of preparation method of above-mentioned Chinese medicine preparation.
Last technical scheme of the present invention is as follows:
A kind of Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease, it is the medicated powder be made up of the medicine material of following weight percentage ratio: Rhizoma Chuanxiong 5 ~ 11%, Radix Salviae Miltiorrhizae 5 ~ 11%, Radix Angelicae Sinensis 2 ~ 8%, Herba Leonuri 2 ~ 8%, Ramulus Uncariae Cum Uncis 7 ~ 13%, Rhizoma Corydalis 7 ~ 13%, Semen Ziziphi Spinosae 5 ~ 11%, Radix Bupleuri 5 ~ 11%, Rhizoma Polygonati 5 ~ 11%, the Radix Astragali 5 ~ 11%, Herba Epimedii 5 ~ 11%, Fructus Schisandrae Chinensis 5 ~ 11%, Radix Glycyrrhizae 2 ~ 8%, vitamin C 0.5 ~ 1.5%, and each component sum is 100%.
Further, described drug weight degree is: Rhizoma Chuanxiong 8%, Radix Salviae Miltiorrhizae 8%, Radix Angelicae Sinensis 5%, Herba Leonuri 5%, Ramulus Uncariae Cum Uncis 10%, Rhizoma Corydalis 10%, Semen Ziziphi Spinosae 8%, Radix Bupleuri 8%, Rhizoma Polygonati 8%, the Radix Astragali 8%, Herba Epimedii 8%, Fructus Schisandrae Chinensis 8%, Radix Glycyrrhizae 5%, vitamin C 1%.
Further, described medicated powder granularity is less than 80 orders.
After of the present invention, a technical scheme is as follows:
The preparation method of the Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease, comprises following preparation process:
(1) vitamin C medicated powder is pulverized into after taking vitamin C by weight percentage;
(2) precentagewise mixes after taking these raw material of Chinese medicine of Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Herba Leonuri, Ramulus Uncariae Cum Uncis, Rhizoma Corydalis, Semen Ziziphi Spinosae, Radix Bupleuri, Rhizoma Polygonati, the Radix Astragali, Herba Epimedii, Fructus Schisandrae Chinensis and Radix Glycyrrhizae;
(3) mixed Chinese medicine is carried out pulverization process;
(4) Chinese medicine powder after pulverizing is crossed 60 ~ 80 mesh sieves;
(5) Chinese medicine powder after screening is carried out microwave drying, then after mixing homogeneously with vitamin C medicated powder and get final product.
Further, in step (4), the Chinese medicine powder after pulverizing is crossed 80 mesh sieves.
Further, in step (5), drying time is 20 ~ 40 minutes.
Compared with prior art, the beneficial effect that the present invention has is:
The Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease of the present invention, except vitamin C, all the other all select Chinese medicine material, do not have the Drug resistance of chemical agent, drug resistance and poisoning residual.Rhizoma Chuanxiong in preparation has activating QI to alleviate the depression, promoting blood circulation and stopping pain, antibacterial effect; Radix Angelicae Sinensis energy benefiting blood and regulating blood circulation, enhancing immunity; Ramulus Uncariae Cum Uncis has heat clearing away suppressing the hyperactive liver, the effect of the arresting convulsion that relieves dizziness, high fever, infantile convulsions, epilepsy, etc.; Rhizoma Corydalis is warm in nature, acrid in the mouth is bitter, has effect of blood circulation promoting and blood stasis dispelling, promoting the circulation of QI to relieve pain; Semen Ziziphi Spinosae sugariness is put down, and has nourishing the liver, mind calming, calms the nerves, effect of arresting sweating; Radix Salviae Miltiorrhizae energy promoting blood flow to regulate menstruation, stasis-dispelling and pain-killing, removing heat from blood eliminating carbuncle, the relieving restlessness that clears away heart-fire, nourishing blood to tranquillize the mind; Radix Bupleuri have induce sweat bring down a fever, effect of dispersing the stagnated live-QI to relieve the stagnation of QI; Rhizoma Polygonati has boosting qi and nourishing yin, spleen invigorating, lung moistening, effect of kidney tonifying; The icariin contained in Herba Epimedii, can promote hemopoietic function, immunologic function and bone metabolism, has antibacterial, the effect such as antiviral, antiinflammatory; Fructus Schisandrae Chinensis energy energy supplementing QI for promoting the production of body fluid, the kidney invigorating nourish heart, it is astringent or styptic treatment for spontaneous sweating to restrain, and build up one's resistance to disease; The Radix Astragali is QI invigorating good medicine, builds up resistance.The medicament of this formula is used for the treatment of Trionyx sinensis (Wiegmann) stress disease, and therapeutic effect is good, is generally connected to feed by the Trionyx sinensis (Wiegmann) of morbidity and just can cure for 3 ~ 4 days.
The preparation method of the Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease of the present invention, vitamin C medicated powder is pulverized into after taking vitamin C by weight percentage, mix after taking various Chinese medicine by weight percentage, again mixed Chinese medicine pulverized and cross 80 mesh sieves, after microwave drying is mixed homogeneously with vitamin C medicated powder again and get final product, effective ingredient when this pharmaceutical preparation decocts in Chinese medicine can better be dissolved in water, the Trionyx sinensis (Wiegmann) that after being mixed with medicinal liquid by feedstuff, feeding is sick, facilitates its edible absorption.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme of the present invention is described in further detail, but does not form any limitation of the invention.
Embodiment 1
The Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease, each weight percentages of components is: Rhizoma Chuanxiong 5%, Radix Salviae Miltiorrhizae 11%, Radix Angelicae Sinensis 2%, Herba Leonuri 8%, Ramulus Uncariae Cum Uncis 7%, Rhizoma Corydalis 13%, Semen Ziziphi Spinosae 5%, Radix Bupleuri 11%, Rhizoma Polygonati 5%, the Radix Astragali 11%, Herba Epimedii 5%, Fructus Schisandrae Chinensis 11%, Radix Glycyrrhizae 5%, vitamin C 1%.
The preparation method of Chinese medicine preparation of the present invention, comprises the steps:
(1) vitamin C medicated powder is pulverized into after taking vitamin C by weight percentage.Select common tablet vitamin C, and this vitamin C tablet easily pulverizes, easily water-soluble, do not need through pulverization process.
(2) precentagewise takes after precentagewise takes these raw material of Chinese medicine of Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Herba Leonuri, Ramulus Uncariae Cum Uncis, Rhizoma Corydalis, Semen Ziziphi Spinosae, Radix Bupleuri, Rhizoma Polygonati, the Radix Astragali, Herba Epimedii, Fructus Schisandrae Chinensis and Radix Glycyrrhizae and mixes.The medicament be prepared from of being arranged in pairs or groups by this few taste medical material is used for the treatment of Trionyx sinensis (Wiegmann) stress disease, makes therapeutic effect good.
(3) mixed Chinese medicine is carried out pulverization process, each Chinese medicine mixed effect after pulverizing is better.Mixed Chinese medicine is broken into powder, and when medicated powder is decocted with water, effective ingredient is better dissolved in water, so that ill Trionyx sinensis (Wiegmann) better absorbs.
(4) Chinese medicine powder after pulverizing is crossed 60 ~ 80 mesh sieves, generally the Chinese medicine powder after pulverizing is crossed 80 mesh sieves, make the utilization rate of Chinese medicine powder higher like this.
(5) by screening after Chinese medicine powder carry out microwave drying after and get final product.Wherein, drying time is 20 ~ 40 minutes.Microwave drying carries out usually in microwave tunnel, can control suitable microwave intensity, reaches quick-drying object.By Chinese medicine is carried out microwave drying treatment, make Chinese medicine drier conveniently deposit and microwave also with bactericidal action, killing bacteria can not needed to increase sterilization steps in addition.
A kind of pharmaceutical preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease of the present invention, except vitamin C, all the other all select Chinese medicine material, do not have the Drug resistance of chemical agent, drug resistance and poisoning residual.Wherein, Radix Salviae Miltiorrhizae in medicament, Rhizoma Corydalis, Radix Salviae Miltiorrhizae and Herba Leonuri have the effect of invigorating blood circulation, the soothing the liver suppressing the hyperactive liver of Ramulus Uncariae Cum Uncis in medicament, Semen Ziziphi Spinosae and Radix Bupleuri energy, Radix Angelicae Sinensis, Rhizoma Polygonati, the Radix Astragali, Herba Epimedii and Fructus Schisandrae Chinensis can improve immunity, the medicament of this formula is used for the treatment of Trionyx sinensis (Wiegmann) stress disease, therapeutic effect is good, is generally connected to feed by the Trionyx sinensis (Wiegmann) of morbidity and just can cure for 3 ~ 4 days.
Using method: when finding that the Trionyx sinensis (Wiegmann) in Trionyx sinensis Wiegmann pond suffers from stress disease, claimed by medicated powder 80g as a standard part, a kind of pharmaceutical preparation one standard part medicated powder for the treatment of Trionyx sinensis (Wiegmann) stress disease of the present invention is put in marmite by every only ill Trionyx sinensis (Wiegmann) at every turn, to add in 150ml water slow fire boiling after 2 hours, taking liquid removes slag and cools, be mixed into after 4% Trionyx sinensis (Wiegmann) feedstuff stirs and throw something and feed, sooner or later respectively once, within 3 ~ 4 days, cure.
Embodiment 2
The Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease, each weight percentages of components is: Rhizoma Chuanxiong 8%, Radix Salviae Miltiorrhizae 8%, Radix Angelicae Sinensis 5%, Herba Leonuri 5%, Ramulus Uncariae Cum Uncis 10%, Rhizoma Corydalis 10%, Semen Ziziphi Spinosae 8%, Radix Bupleuri 8%, Rhizoma Polygonati 8%, the Radix Astragali 8%, Herba Epimedii 8%, Fructus Schisandrae Chinensis 8%, Radix Glycyrrhizae 5%, vitamin C 1%.
Preparation method is identical with embodiment 1 with using method.
Embodiment 3
The Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease, each weight percentages of components is: Rhizoma Chuanxiong 11%, Radix Salviae Miltiorrhizae 5%, Radix Angelicae Sinensis 8%, Herba Leonuri 2%, Ramulus Uncariae Cum Uncis 13%, Rhizoma Corydalis 7%, Semen Ziziphi Spinosae 11%, Radix Bupleuri 5%, Rhizoma Polygonati 11%, the Radix Astragali 5%, Herba Epimedii 11%, Fructus Schisandrae Chinensis 5%, Radix Glycyrrhizae 5%, vitamin C 1%.
Preparation method is identical with embodiment 1 with using method.
Embodiment 4
The Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease, each weight percentages of components is: Rhizoma Chuanxiong 6%, Radix Salviae Miltiorrhizae 10%, Radix Angelicae Sinensis 3%, Herba Leonuri 7%, Ramulus Uncariae Cum Uncis 8%, Rhizoma Corydalis 12%, Semen Ziziphi Spinosae 6%, Radix Bupleuri 10%, Rhizoma Polygonati 6%, the Radix Astragali 10%, Herba Epimedii 6%, Fructus Schisandrae Chinensis 10%, Radix Glycyrrhizae 4.5%, vitamin C 1.5%.
Preparation method is identical with embodiment 1 with using method.
Rhizoma Chuanxiong 5 ~ 11%, Radix Salviae Miltiorrhizae 5 ~ 11%, Radix Angelicae Sinensis 2 ~ 8%, Herba Leonuri 2 ~ 8%, Ramulus Uncariae Cum Uncis 7 ~ 13%, Rhizoma Corydalis 7 ~ 13%, Semen Ziziphi Spinosae 5 ~ 11%, Radix Bupleuri 5 ~ 11%, Rhizoma Polygonati 5 ~ 11%, the Radix Astragali 5 ~ 11%, Herba Epimedii 5 ~ 11%, Fructus Schisandrae Chinensis 5 ~ 11%, Radix Glycyrrhizae 2 ~ 8%, vitamin C 0.5 ~ 1.5%
Embodiment 5
The Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease, each weight percentages of components is: Rhizoma Chuanxiong 7%, Radix Salviae Miltiorrhizae 9%, Radix Angelicae Sinensis 6%, Herba Leonuri 4%, Ramulus Uncariae Cum Uncis 8%, Rhizoma Corydalis 12%, Semen Ziziphi Spinosae 7%, Radix Bupleuri 9%, Rhizoma Polygonati 6%, the Radix Astragali 10%, Herba Epimedii 9%, Fructus Schisandrae Chinensis 7%, Radix Glycyrrhizae 5.5%, vitamin C 0.5%.
Preparation method is identical with embodiment 1 with using method.
Contrast test
Test site is selected in Jingtai, Wuzhou plant of Yang Bie Specialty Co-operative Organization and carries out.After in July, 2013, along with temperature raises, Trionyx Sinensis pond middle water level reaches 28 ~ 30 DEG C, and the Trionyx Sinensis part in Trionyx sinensis Wiegmann pond shows successively has dizzy spells, often show as and emerge, head rocks on the water surface, is far from being tranquil, and checks its health, find that whole body body surface is intact, immaculate, without ulcer, tentatively concludes that they suffer from stress disease.
Prevention and controls
Test, by substantially identical for two specifications, uses the Trionyx sinensis pond of identical water system, and the Trionyx sinensis (Wiegmann) quantity of cultivation is identical and the Trionyx sinensis Wiegmann pond that disease condition is also substantially identical is set as test Trionyx sinensis pond, and arranging No. 1 Trionyx sinensis Wiegmann pond is test group, and No. 2 Trionyx sinensis Wiegmann ponds are matched group.When on-test, record the number of ill Trionyx sinensis in 1, No. 2 Trionyx sinensis Wiegmann pond respectively.Use the Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease of the present invention to treat in No. 1 Trionyx sinensis Wiegmann pond, getting 1 standard part medicated powder by every Trionyx sinensis (Wiegmann) in Trionyx sinensis Wiegmann pond is put in marmite, slow fire boiling is added in 150ml water after 2 hours by every 1 standard part medicated powder, taking liquid removes slag and cools, be mixed into after 4% Trionyx sinensis (Wiegmann) feedstuff stirs and throw something and feed, sooner or later each once, after being used in conjunction 3 days, observing and record the situation of Trionyx Sinensis in No. 1 Trionyx sinensis Wiegmann pond.No. 2 Trionyx sinensis Wiegmann ponds use merely vitamin C tablet to treat, add after vitamin C 3 ~ 4g takes vitamin C tablet by per kilogram feedstuff and pulverize into vitamin C medicated powder, put into after feedstuff stirs and throw something and feed, continuous 7 days,, observe and record the situation of Trionyx Sinensis in No. 1 Trionyx sinensis Wiegmann pond.By as follows for the Trionyx sinensis (Wiegmann) therapeutic outcome comparable situation in 1, No. 2 Trionyx sinensis Wiegmann pond:
Results contrast after experimental group and matched group medication
According to data analysis in results contrast table after test group and contrast groups medicine, sick Trionyx sinensis Wiegmann healing time in No. 1 Trionyx sinensis Wiegmann pond is shorter than the sick Trionyx sinensis Wiegmann healing time in No. 2 Trionyx sinensis Wiegmann ponds, the sick Trionyx sinensis Wiegmann in No. 1 Trionyx sinensis Wiegmann pond after 3 days substantially the mental status, ingest and live all normal, whole ill Trionyx sinensis is cured, the sick Trionyx sinensis Wiegmann major part in No. 2 Trionyx sinensis Wiegmann ponds after 3 days spirit improves, ingest increase, Trionyx sinensis major part has sneaked into water-bed rest, and most of ill Trionyx sinensis takes a turn for the better; Sick Trionyx sinensis Wiegmann in No. 2 Trionyx sinensis Wiegmann ponds is treated and all cures afterwards for 7 days, and No. 1 Trionyx sinensis Wiegmann pond does not find new ill Trionyx sinensis yet.Comprehensive above relative analysis data draw, use the Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease of the present invention to treat, therapeutic effect is better.
The Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease of the present invention is except vitamin C, and all the other all select Chinese medicine material, do not have the Drug resistance of chemical agent, drug resistance, and medicine can not be made residual on the body of Trionyx sinensis (Wiegmann).
The foregoing is only preferred embodiment of the present invention, all do within the scope of the spirit and principles in the present invention any amendment, equivalent to replace and improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. treat the Chinese medicine preparation of Trionyx sinensis (Wiegmann) stress disease for one kind, it is characterized in that, it is the medicated powder be made up of the medicine material of following weight percentage ratio: Rhizoma Chuanxiong 5 ~ 11%, Radix Salviae Miltiorrhizae 5 ~ 11%, Radix Angelicae Sinensis 2 ~ 8%, Herba Leonuri 2 ~ 8%, Ramulus Uncariae Cum Uncis 7 ~ 13%, Rhizoma Corydalis 7 ~ 13%, Semen Ziziphi Spinosae 5 ~ 11%, Radix Bupleuri 5 ~ 11%, Rhizoma Polygonati 5 ~ 11%, the Radix Astragali 5 ~ 11%, Herba Epimedii 5 ~ 11%, Fructus Schisandrae Chinensis 5 ~ 11%, Radix Glycyrrhizae 2 ~ 8%, vitamin C 0.5 ~ 1.5%, and each component sum is 100%.
2. the Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease according to claim 1, it is characterized in that, described drug weight degree is: Rhizoma Chuanxiong 8%, Radix Salviae Miltiorrhizae 8%, Radix Angelicae Sinensis 5%, Herba Leonuri 5%, Ramulus Uncariae Cum Uncis 10%, Rhizoma Corydalis 10%, Semen Ziziphi Spinosae 8%, Radix Bupleuri 8%, Rhizoma Polygonati 8%, the Radix Astragali 8%, Herba Epimedii 8%, Fructus Schisandrae Chinensis 8%, Radix Glycyrrhizae 5%, vitamin C 1%.
3. the Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease according to claim 1, is characterized in that, described medicated powder granularity is less than 80 orders.
4. treat the preparation method of the Chinese medicine preparation of Trionyx sinensis (Wiegmann) stress disease described in claim 1 or 2, it is characterized in that, comprise following preparation process:
(1) vitamin C medicated powder is pulverized into after taking vitamin C by weight percentage;
(2) precentagewise mixes after taking these raw material of Chinese medicine of Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Herba Leonuri, Ramulus Uncariae Cum Uncis, Rhizoma Corydalis, Semen Ziziphi Spinosae, Radix Bupleuri, Rhizoma Polygonati, the Radix Astragali, Herba Epimedii, Fructus Schisandrae Chinensis and Radix Glycyrrhizae;
(3) mixed Chinese medicine is carried out pulverization process;
(4) Chinese medicine powder after pulverizing is crossed 60 ~ 80 mesh sieves;
(5) Chinese medicine powder after screening is carried out microwave drying, then after mixing homogeneously with vitamin C medicated powder and get final product.
5. the preparation method of the Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease according to claim 4, is characterized in that, in step (4), the Chinese medicine powder after pulverizing is crossed 80 mesh sieves.
6. the preparation method of the Chinese medicine preparation for the treatment of Trionyx sinensis (Wiegmann) stress disease according to claim 4, is characterized in that, in step (5), drying time is 20 ~ 40 minutes.
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Effective date of registration: 20180927 Address after: 543000 303 unit, 2 unit 30, Yin Hu South Road, Wuzhou, the Guangxi Zhuang Autonomous Region. Patentee after: Wuzhou Dexiang Ecological Agriculture Technology Development Co., Ltd. Address before: 543003 Zhou Yi house, Ren Yi village, Changshui District, Wuzhou, the Guangxi Zhuang Autonomous Region Patentee before: WUZHOU CITY JINGTAI TURTLE PROFESSIONAL COOPERATIVES |