CN105054276A - Method for manufacturing added bases for burley tobaccos - Google Patents
Method for manufacturing added bases for burley tobaccos Download PDFInfo
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- CN105054276A CN105054276A CN201510420420.XA CN201510420420A CN105054276A CN 105054276 A CN105054276 A CN 105054276A CN 201510420420 A CN201510420420 A CN 201510420420A CN 105054276 A CN105054276 A CN 105054276A
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Abstract
The invention relates to original natural amino acid of tobacco leaves and application of the original natural amino acid to burley tobaccos. Waste powder from tobacco fields and buffer liquor are mixed with each other, 0.1%-0.3% protease is added into mixtures of the waste powder and the buffer liquor, a pH (potential of hydrogen) value of a reaction system is regulated until the pH value of the reaction system is 4.6-6.0, the reaction system is heated until the temperature of the reaction system is 40-60 DEG C, the reaction system continues reacting for 4-6h and is cooled, then protease with the mass equal to the mass of the reaction system is added into the reaction system until the protease is completely degraded, the reaction system is cooled and then is filtered and concentrated to obtain degradation products which are bases, the bases are mixed with carbohydrate liquid by a certain proportion and then are applied to the burley tobacco leaves, and the burley tobaccos are baked and processed, so that the sensory quality can be greatly enhanced. The original natural amino acid and the application have the advantages that the waste powder from the tobacco fields is used as a raw material, the degradation products which are obtained by means of sufficient enzymatic hydrolysis mainly are amino acid mixtures, the total protein contents of the degradation products are 15.25%, and yield of amino acid nitrogen is 2.16%; technological procedures are simple, the original natural amino acid is low in cost, and resources can be comprehensively utilized.
Description
one, technical field
The present invention relates to a kind of burley tobaccos feed in raw material material base preparation method, this product is applied in burley tobaccos blade, the organoleptic quality of burley tobaccos can be improved.
two, background technology
Tobacco business is the important sources of national property tax, is also economic important component part.Along with becoming increasingly conspicuous and China's entry into the WTO of smoking and healthy contradiction, development low tar blended type cigarette is imperative, burley tobaccos are as a kind of important source material cigarette of blended type cigarette, also more and more popular to its research, but about burley tobaccos reinforced material base, the research adding natural amino acid is especially then few.
Burley tobaccos belong to the common safflower tobacco of Solanaceae, with flue-cured tobacco, Turkish tobaccos be called the three major types type of tobacco.But it and flue-cured tobacco, Turkish tobaccos have significantly different in every respect.Burley tobaccos short texture, fillibility is strong, and moisture pick-up properties is higher, and saccharide compound content is low, total nitrogen and nicotine content high, sense organ has unique airing cigarette fragrance.Because burley tobaccos contents of saccharide is on the low side, total nitrogen and nicotine content higher, the upper flue gas meta-alkalescence of the burley tobaccos aesthetic quality without process of feeding in raw material, cure, excitant is bigger than normal, assorted gas and ammonia heavier, fragrance is dull, and strength is higher.Therefore, burley tobaccos have to pass through reinforced, cure process and just can use.The feed liquid of usual burley tobaccos is based on carbohydrate, humectant and amino acid.Amino acid can regulate sugared nitrogen ratio and the pH value of burley tobaccos, itself and recuding sugars generation enzymatic and non-enzymatic catalysis (Maillard) can also be impelled to react, and then forming the heterocyclic compound composition of a series of pyrroles, pyrazine and furans, the fragrance of these compositions to tobacco has material impact.Through the burley tobaccos of process of feeding in raw material, cure, burley tobaccos feature cigarette perfume is outstanding, flue gas acid-base balance, and coherency and fine and smooth degree improve, and assorted gas alleviates, pure in mouth feel.
The effect of single or simple combination of amino acids in burley tobaccos feed in raw material obviously will be weaker than the mixture of several amino acids.Meanwhile, tobacco agriculture produces a large amount of tobacco leaf discarded objects in producing, these discarded objects as utilized, not only contaminated environment but also cause the wasting of resources.
three, summary of the invention
The present invention is intended to the preparation and application method for burley tobaccos provide burley tobaccos to feed in raw material material base, and this natural material base still extracts from tobacco in field discarded object, has no side effect.Utilize the free amino acid in proteasome degradation liquid, through process after can arrive a kind of function raw material as burley tobaccos.After these tobacco in field discarded objects are carried out the process of science by the present invention, natural tobacco origin amino acid composition can be made, as burley tobaccos reinforced material base, improve the total quality of burley tobaccos, not only take full advantage of tobacco resource, turn improve the quality of burley tobaccos.
Technical scheme of the present invention is: a kind of burley tobaccos feed in raw material and expect that the preparation method of base carries out according to the following steps:
(1) Feedstock treating: field trash is ground into Powdered, 20 ~ 80 orders sieve, and lucifuge sealing is preserved;
(2) preparation of compound amino acid:
1) for the first time degrade: by tobacco in field waste-powder with citric acid-sodium citrate buffer with 1:8 ~ 1:12(g/mL) mass volume ratio fully mix, playing heavy industry citric acid-sodium citrate buffer pH value is 4.6 ~ 6.0, add 0.1% ~ 0.3% part of protease of raw material weight, mix, heating water bath 40 ~ 60 DEG C, extraction is stirred, and mixing time is 4 ~ 6h, cooling.
2) secondary degraded: add the protease with identical weight of degrading for the first time, extraction is stirred, cooling, filters.
3) subsequent treatment; Again extract, go out enzyme, merging filtrate, and the filtrate of gained is concentrated into certain volume, obtains the proteasome degradation liquid of tobacco in field waste-powder.
(3) preparation of reinforced material base: by the ethanol of above-mentioned gained degradation product certain mass mark dilution 2 ~ 6 times, centrifugal 1 ~ 20min on 3000rpm centrifuge, the supernatant of gained solution is the reinforced material base of burley tobaccos.
Described tobacco in field discarded object refers to the part that can not be used for tobacco curing, comprise tobacco stem, offal, the upper tobacco leaf of tobacco plant, vein, fireworks, axillalry bud and cannot be used for cigarette inferior grade or etc. outer level tobacco leaf.
Described protease is the papain of food-grade solid powdery, bromelain, flavor protease, alkali protease, acid protease and complex enzyme.
Described citric acid and sodium citrate solution concentration are 0.1mol/L.
Described extraction step is again in filter residue, add the distilled water that mass volume ratio is 1:1 ~ 1:4, stirs extraction 10 ~ 30min.
The described enzyme that goes out is boiling water bath 5 ~ 30min.
Application process: the invert sugar of mass concentration 65% is mixed with prepared material base, reinforced process is carried out to burley tobaccos sample, after applying, the addition of invert sugar (65%) is made to be 4% of quality of tobacco, the addition of made base of getting the raw materials ready is respectively 0%, 0.05%, 0.2%, 0.4%, 0.5% of quality of tobacco, add appropriate water simultaneously, make reinforced rear tobacco leaf moisture content reach about 35%.Adopt conventional burley tobaccos syllogic baking process to carry out curing process, the burley tobacco leaf after the process of difference material base applied amount is eased back, chopping, baking silk, roll into cigarette.The climatic chamber that cigarette sample puts into temperature 22 ± 1 DEG C and relative humidity (RH) 60 ± 2% balances 48h, carries out sensory evaluating smoking.Smoking result is in table 1.
Described protease is the papain of food-grade solid powdery, bromelain, flavor protease, alkali protease, acid protease and complex enzyme.Described citric acid and sodium citrate solution concentration are 0.1mol/L.The described stirring in water bath time is 4 ~ 6h, and described extraction step is again the water adding 1 ~ 4 times of volume in filter residue, stirs extraction 10 ~ 30min.The described enzyme that goes out is boiling water bath 5 ~ 30min.
Preparation process of the present invention is simple, can accomplish resource reutilization, not only economy but also environmental protection with tobacco in field discarded object as raw material.And the degradation solution of this gained can directly as the reinforced material base of burley tobaccos.In addition, degradation solution of the present invention as the base fluid of Maillard reaction, can carry out Maillard reaction, thus contributes to perfuming cigarette.
Compared with prior art, the present invention has following advantage:
1, the tobacco aromatics prepared of the present invention, adopt tobacco in field discarded object, effectively prevent that amino acid difference that xenogenesis raw material causes and kind lack that the product excitant caused is large, the fragrant shortcoming such as inharmonious of cigarette, not only remain the Original amino of tobacco leaf, but also reduction excitant, increase the fragrance of cigarette.
2, the present invention is simple to operate, is applicable to suitability for industrialized production, has good prospects.
Detailed description of the invention
Maximum feature of the present invention there is provided the reinforced tobacco origin spices material base of a kind of burley tobaccos, this perfume base abundance, preparation technology is simple, original processing technology is not changed during interpolation, improve the organoleptic quality of burley tobaccos, and tobacco in field discarded object is fully used, therefore there is the meaning and value of actual production.
Below in conjunction with detailed description of the invention, the present invention is further illustrated.
Embodiment 1
1, in the 1000mL round-bottomed flask that stirrer is housed, add 40g tobacco in field discarded object powder, 400mL citric acid-sodium citrate buffer, 0.12g papain successively, regulate pH value of solution to be 5.4,40 DEG C of heating water baths, and continue reaction 6h, after cooling, continue to add 0.12g papain, stir 30min, after cooling, boiling water bath 10min, filters, is concentrated into 25mL, obtain rufous degradation product, be mainly amino acid whose mixture.
2, get 25mL rufous degradation product and carry out mixing (wherein 25mL rufous degradation product to the applying scope of burley tobaccos greatly about 0.05 ~ 0.2%) with the invert sugar of mass concentration 65%, after applying, the addition of invert sugar (65%) is 1.6g, the addition of made base of getting the raw materials ready is 0.2g, add the water of 30mL simultaneously, make reinforced rear tobacco leaf moisture content reach about 35%.Adopt conventional burley tobaccos syllogic baking process to carry out curing process, the burley tobacco leaf after the process of difference material base applied amount is eased back, chopping, baking silk, roll into cigarette., get a material based on medical watering can, be evenly sprayed on burley tobaccos sample, the climatic chamber that cigarette sample puts into temperature 22 DEG C and relative humidity (RH) 60% balances 48h, carries out sensory evaluating smoking.Smoking result is in table 2.
Embodiment 2
1, in the 1000mL round-bottomed flask that stirrer is housed, add 40g tobacco in field discarded object powder, 400mL citric acid-sodium citrate buffer, 0.10g bromelain successively, regulate pH value of solution to be 5.2,60 DEG C of heating water baths, and continue reaction 4h, after cooling, continue to add 0.10g bromelain (making the complete enzymolysis of its protein), stir 30min, after cooling, 100 DEG C of water-bath 10min, filter, are concentrated into 25mL, obtain rufous degradation product, be mainly amino acid whose mixture.
2, get 25mL rufous degradation product and carry out mixing (wherein 25mL rufous degradation product to the applying scope of burley tobaccos greatly about 0.05 ~ 0.2%) with the invert sugar of mass concentration 65%, after applying, the addition of invert sugar (65%) is 1.6g, the addition of made base of getting the raw materials ready is 0.2g, add the water of 30mL simultaneously, make reinforced rear tobacco leaf moisture content reach about 35%.Adopt conventional burley tobaccos syllogic baking process to carry out curing process, the burley tobacco leaf after the process of difference material base applied amount is eased back, chopping, baking silk, roll into cigarette.Get a material based on medical watering can, be evenly sprayed on burley tobaccos sample, the climatic chamber that cigarette sample puts into temperature 22 DEG C and relative humidity (RH) 60% balances 48h, carries out sensory evaluating smoking.Evaluation and analysis the results are shown in Table 3.
effect:
Burley tobaccos feature cigarette perfume is outstanding, and flue gas acid-base balance, assorted gas alleviates, pure in mouth feel (see table 1).Applied amount scope is with 0.05 ~ 0.5%.
The effect of table 1 difference material base applied amount
| Sample | Consumption/% | Smoking result |
| Contrast | 0 | Flue gas meta-alkalescence, excitant is bigger than normal, assorted gas and ammonia heavier, fragrance is dull, and strength is higher |
| 1# | 0.05 | Note slightly increases, and cigarette perfume (or spice) is slightly dull, and slightly, excitant declines assorted gas, and pleasant impression is more comfortable |
| 2# | 0.25 | Note is graceful, denseer fragrance, and fine and soft degree improves, and assorted gas, excitant alleviate, and pleasant impression is comfortable |
| 3# | 0.4 | Elegant fragrance, dense fragrance, note enriches, and flue gas is fine and soft, and assorted gas obviously alleviates, and pleasant impression is salubrious, clean |
| 4# | 0.5 | Harmony declines, and fragrance is not given prominence to, and flue gas is slightly sour, and pleasant impression has residual |
Table 2 is applied to sensory evaluating smoking's result of burley tobaccos
| Sample | Consumption/% | Smoking result |
| 5# | 0.05～0.2 | Burley tobaccos cigarette perfume is outstanding, and acid-base balance, flue gas is fine and soft, and assorted gas significantly alleviates |
Table 3 is applied to sensory evaluating smoking's result of burley tobaccos
| Sample | Consumption/% | Smoking result |
| 6# | 0.05～0.2 | Burley tobaccos cigarette perfume (or spice) is soft, elegant fragrance, and assorted gas significantly alleviates, and pleasant impression is clean, salubrious |
Claims (6)
1. burley tobaccos feed in raw material and expect a preparation method for base, it is characterized in that described method is carried out according to the following steps:
(1) Feedstock treating: tobacco in field discarded object is ground into Powdered; 20 ~ 80 orders sieve, and lucifuge sealing is preserved;
(2) compound amino acid preparation:
1) for the first time degrade: by tobacco in field waste-powder with citric acid-sodium citrate buffer with 1:8 ~ 1:12(g/mL) mass volume ratio fully mix, playing heavy industry citric acid-sodium citrate buffer pH value is 4.6 ~ 6.0, add 0.1% ~ 0.3% part of protease of raw material weight, mix, heating water bath 40 ~ 60 DEG C, extraction is stirred, and mixing time is 4 ~ 6h, cooling;
2) secondary degraded: add the protease with identical weight of degrading for the first time, extraction is stirred, cooling, filters;
3) subsequent treatment; Again extract, go out enzyme, merging filtrate, and the filtrate of gained is concentrated into certain volume, obtains the proteasome degradation liquid of tobacco in field waste-powder;
(3) preparation of reinforced material base: by the ethanol of above-mentioned gained degradation product certain mass mark dilution 2 ~ 6 times, centrifugal 1 ~ 20min on 3000rpm centrifuge, the supernatant of gained solution is the reinforced material base of burley tobaccos.
2. a kind of burley tobaccos according to claim 1 feed in raw material and expect the preparation method of base, it is characterized in that: described tobacco in field discarded object refers to the part that can not be used for tobacco curing, comprise tobacco stem, offal, the upper tobacco leaf of tobacco plant, vein, fireworks, axillalry bud and cannot be used for cigarette inferior grade or etc. outer level tobacco leaf.
3. a kind of burley tobaccos according to claim 1 feed in raw material the preparation method of material base, it is characterized in that: described protease is the papain of food-grade solid powdery, bromelain, flavor protease, alkali protease, acid protease and complex enzyme.
4. a kind of burley tobaccos according to claim 1 feed in raw material and expect the preparation method of base, it is characterized in that: described citric acid and sodium citrate solution concentration are 0.1mol/L.
5. a kind of burley tobaccos according to claim 1 feed in raw material and expect the preparation method of base, it is characterized in that: described extraction step is again in filter residue, add the distilled water that mass volume ratio is 1:1 ~ 1:4, stir extraction 10 ~ 30min.
6. a kind of burley tobaccos according to claim 1 feed in raw material and expect the preparation method of base, it is characterized in that: the described enzyme that goes out is boiling water bath 5 ~ 30min.
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| CN201510420420.XA CN105054276A (en) | 2015-07-17 | 2015-07-17 | Method for manufacturing added bases for burley tobaccos |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105595406A (en) * | 2016-01-07 | 2016-05-25 | 河南中烟工业有限责任公司 | Compound enzyme preparation for adjusting quality of tobacco leaves and application thereof |
| CN108451004A (en) * | 2018-01-30 | 2018-08-28 | 湖北中烟工业有限责任公司 | The methods and applications of burley tobaccos reactant are prepared using fruit extract |
| CN114751944A (en) * | 2022-03-31 | 2022-07-15 | 贵州省烟草科学研究院 | Preparation method of proline-glucose Amadori compound |
| CN115381124A (en) * | 2022-09-27 | 2022-11-25 | 江苏中烟工业有限责任公司 | Method for improving adaptability of burley tobacco in flue-cured tobacco type cigarette |
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| CN101940361A (en) * | 2010-10-11 | 2011-01-12 | 广东中烟工业有限责任公司 | Processing method of waste tobacco |
| CN102247009A (en) * | 2011-07-12 | 2011-11-23 | 广东中烟工业有限责任公司 | Maillard reaction process for tobacco stalk extract |
| CN104188093A (en) * | 2014-08-13 | 2014-12-10 | 安徽中烟工业有限责任公司 | Preparation method and application of tobacco additive deriving from burley tobacco |
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2015
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101940361A (en) * | 2010-10-11 | 2011-01-12 | 广东中烟工业有限责任公司 | Processing method of waste tobacco |
| CN102247009A (en) * | 2011-07-12 | 2011-11-23 | 广东中烟工业有限责任公司 | Maillard reaction process for tobacco stalk extract |
| CN104188093A (en) * | 2014-08-13 | 2014-12-10 | 安徽中烟工业有限责任公司 | Preparation method and application of tobacco additive deriving from burley tobacco |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105595406A (en) * | 2016-01-07 | 2016-05-25 | 河南中烟工业有限责任公司 | Compound enzyme preparation for adjusting quality of tobacco leaves and application thereof |
| CN108451004A (en) * | 2018-01-30 | 2018-08-28 | 湖北中烟工业有限责任公司 | The methods and applications of burley tobaccos reactant are prepared using fruit extract |
| CN108451004B (en) * | 2018-01-30 | 2020-09-08 | 湖北中烟工业有限责任公司 | Method for preparing burley tobacco reactant by using fruit extract and application |
| CN114751944A (en) * | 2022-03-31 | 2022-07-15 | 贵州省烟草科学研究院 | Preparation method of proline-glucose Amadori compound |
| CN114751944B (en) * | 2022-03-31 | 2024-06-04 | 贵州省烟草科学研究院 | Preparation method of proline-glucose Amadori compound |
| CN115381124A (en) * | 2022-09-27 | 2022-11-25 | 江苏中烟工业有限责任公司 | Method for improving adaptability of burley tobacco in flue-cured tobacco type cigarette |
| CN115381124B (en) * | 2022-09-27 | 2023-10-27 | 江苏中烟工业有限责任公司 | Method for improving suitability of burley tobacco in flue-cured tobacco type cigarettes |
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