CN105053644A - Application of schizochytrium limacinum wall-breaking cell wall slurry on aquatic product fry seed production - Google Patents
Application of schizochytrium limacinum wall-breaking cell wall slurry on aquatic product fry seed production Download PDFInfo
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Abstract
The invention discloses an application of a schizochytrium limacinum wall-breaking cell wall slurry on aquatic product fry seed production. In the aquatic product fry cultivation usage, 200-250ml of schizochytrium limacinum wall-breaking cell wall slurry is added in each kilogram of fry, then is mixed and uniformly stirred, the wall-breaking cell wall slurry is stand for cooling for 15-20 minutes, and can be used for feeding after being fully absorbed. The schizochytrium limacinum wall-breaking cell wall slurry is used as a feeding material for 15-20 days, growth of beneficial bacteria in the intestine can be effectively promoted, fry is strong, survival rate of the fry can reach more than 83% or can be reached as high as more than 93%, compared with a contrast group without addition of the schizochytrium limacinum wall-breaking cell wall slurry, average survival rate can be increased to 24.53-34.53% respectively, the schizochytrium limacinum wall-breaking cell wall slurry has the advantages of economy and utility, environmental protection, good technical practicality, and obvious economic benefit, and is suitable for popularization and application of an aquatic product culture industry.
Description
Technical field
The present invention relates to the application of a kind of schizochytrium limacinum broken cells wall slurries on aquatic fish seed rearing.
Technical background
Schizochytrium limacinum, also known as splitting kettle algae, belongs to a class marine fungi of Eumycota (Eumycota), Oomycete (Oomycetes), Saprolegniales (Saprolegniales), thraustochytriale section (Thraustochytriaceae), unicellular, spherical.Fragmentation vibrios cell can accumulate a large amount of active material useful to human body, as: the unrighted acid DHA content in its total fat is very high, reaches 35% ~ 68%, and the aliphatic acid of more than 90% is the neutral fats existed as a triglyceride.Also have a small amount of EPA, ARA, DPA etc., the content of fatty acid of similar is low, easy separation and purification.Especially its cell membrane is rich in many rich nutritive materials such as protein, immune polysaccharide, carotenoid, vitamin, mineral matter, phosphatide and about 0.3% grease (mainly DHA, ARA, DPA etc.).Schizochytrium limacinum is formally classified as a kind of important feedstuff by agricultural sector in 2013, and feed (aquatic feeds, animal feed etc.) sector application has tempting development space.But directly utilize schizochytrium limacinum broken cells wall slurry as a kind of feedstuff and production on aquatic fry growing seed rearing and promote and then there is no precedent of appearing in the newspapers.
Summary of the invention
The object of the invention is to provide the application of a kind of schizochytrium limacinum broken cells wall slurries on aquatic fish seed rearing, and mark seedling motility rate is improved respectively, and economic benefit is obvious.
In order to reach above-mentioned purpose, the present invention is realized by following technical measures:
The application of a kind of schizochytrium limacinum broken cells wall slurries on aquatic fish seed rearing, in aquatic fish seed rearing uses, 200 ~ 250ml schizochytrium limacinum broken cells wall slurries are added in per kilogram seedling material, mixing and stirring, leave standstill shady and cool 15 ~ 20 minutes, can arrange to throw something and feed after it fully absorbs.
Described schizochytrium limacinum broken cells wall slurries are: schizochytrium limacinum unicellular employing aqueous enzymatic method broken wall, it is unicellular after enzymatic shell-broken, DHA grease is released, shell-broken liquid is follow-up through deactivation, neutralization and centrifugation (three-phase centrifuge), institute obtains oil phase is and is rich in the elementary grease of DHA, and slag is broken cells wall slurries mutually.
Described aqueous enzymatic method technique is: temperature 35 ~ 60 DEG C, pH=6.0 ~ 8.0, and complex enzyme adopts cellulase and neutral proteinase, compositely proportional: (5 ~ 50%): (95 ~ 50%); Addition is treat broken wall zymotic fluid weight 1 ~ 4%, and broken time is 8.0 ~ 14.0 hours, and 65 ~ 95 DEG C of deactivations 30 minutes, then uses food-grade acid (citric acid, hydrochloric acid etc.) to regulate shell-broken liquid pH to 5.0 ~ 6.8.Slag phase (being rich in the unicellular cells wall broken wall slurries of nutritional labeling)-cell membrane slurries can be obtained finally by three phase centrifugation.
In described schizochytrium limacinum broken cells wall slurries, mainly containing protein, amino acid, polysaccharide, mineral matter and micro-DHA etc. material, dry matter content is 5.2 ~ 6.8 at 25 ~ 50g/l, pH.
Described schizochytrium limacinum broken cells wall slurries should add water soluble antioxidants and carry out anti-oxidation protection in storage and transport process; antioxidant can be in vitamin C, sodium ascorbate, sodium isoascorbate and Tea Polyphenols any one or two kinds composite, addition 0.1 ~ 0.4%.
Described schizochytrium limacinum broken cells wall slurry applications is in the seed rearing of grouper, large yellow croaker, mandarin fish, eel and soft-shelled turtle.
The invention has the beneficial effects as follows: schizochytrium limacinum broken cells wall slurries are rich in polysaccharide, protein, vitamin, the composition of the nutrition such as the unrighted acid of mineral matter and part DHA, application process of the present invention is adopted to throw something and feed aquatic products fry seed (grouper, large yellow croaker, mandarin fish etc.), tame and docile material 15 ~ 20 days, can effectively promote intestinal beneficial flora growth, fry is strong, mark shoot survival percent reaches more than 83%, have up to more than 93%, contrast with the control group do not added, average survival improves 24.53 ~ 34.53% respectively, economical and practical, environmental protection, technological applicability is good, economic benefit is obvious, aquaculture industry can be applicable to and apply production.The present invention obtains effective application on the aquatic fish seed rearings such as grouper, large yellow croaker, mandarin fish.
Accompanying drawing explanation
Fig. 1 is process chart of the present invention.
Detailed description of the invention
Fig. 1 and specific embodiment is below coordinated to be described in further detail the present invention.
Embodiment 1:
Environment: cement pit 13 square metres, amounts to preparation 20 mouthfuls (experiment and each 10 mouthfuls of blank group), depth of water 80cm, water temperature 22-26 DEG C;
Salinity: 16 ‰ ~ 17.3 ‰;
Pearl rough gentian seed (lithosporic): 15000 tails, 6 ~ 7cm(05-06 sieves);
Producer's compound feed is selected in test, and mix slag liquid 200 ~ 250ml by per kilogram, soak into feed, the moon dries in the air 15 minutes, throws something and feeds.
Experimental result: experimental group mark seedling average viability is 83%, reaches as high as 93%, blank group mark seedling survival rate is 58.47%, and the mark seedling survival rate of experimental group improves 24.53 ~ 34.53% fully.
Embodiment 2:
Environment: cement pit 13 square metres, amounts to preparation 20 mouthfuls (experiment and each 10 mouthfuls of blank group), depth of water 80cm, water temperature 22-26 DEG C;
Salinity: 16 ‰ ~ 17.3 ‰;
Large yellow croaker seed: 12000 tails, 6 ~ 7cm(05-06 sieves);
Producer's compound feed is selected in test, and mix slag liquid 200 ~ 250ml by per kilogram, soak into feed, the moon dries in the air 15 minutes, throws something and feeds.
Experimental result: experimental group mark seedling survival rate is 83.35%, is up to 89.67%, blank group mark seedling average viability is 63.1%, and the mark seedling survival rate of experimental group improves 20.20 ~ 26.5% fully.
Claims (6)
1. the application of schizochytrium limacinum broken cells wall slurries on aquatic fish seed rearing, it is characterized in that: in aquatic fish seed rearing uses, 200 ~ 250ml schizochytrium limacinum broken cells wall slurries are added in per kilogram seedling material, mixing and stirring, leave standstill shady and cool 15 ~ 20 minutes, after it fully absorbs, namely arrange to throw something and feed.
2. the application of a kind of schizochytrium limacinum broken cells wall slurries as claimed in claim 1 on aquatic fish seed rearing, it is characterized in that: described schizochytrium limacinum broken cells wall slurries are: schizochytrium limacinum unicellular employing aqueous enzymatic method broken wall, it is unicellular after enzymatic shell-broken, DHA grease is released, shell-broken liquid is follow-up through deactivation, neutralization and centrifugation, institute obtains oil phase is and is rich in the elementary grease of DHA, and slag is broken cells wall slurries mutually.
3. the application of a kind of schizochytrium limacinum broken cells wall slurries as claimed in claim 2 on aquatic fish seed rearing, it is characterized in that: described aqueous enzymatic method technique is: temperature 35 ~ 60 DEG C, pH=6.0 ~ 8.0, complex enzyme adopts cellulase and neutral proteinase, compositely proportional: (5 ~ 50%): (95 ~ 50%); Addition is treat broken wall zymotic fluid weight 1 ~ 4%, and broken time is 8.0 ~ 14.0 hours, and 65 ~ 95 DEG C of deactivations 30 minutes, then regulates shell-broken liquid pH to 5.0 ~ 6.8 with food-grade acid; Slag phase-cell membrane slurries can be obtained finally by three phase centrifugation.
4. the application of a kind of schizochytrium limacinum broken cells wall slurries as claimed in claim 1 on aquatic fish seed rearing, is characterized in that: in described schizochytrium limacinum broken cells wall slurries, its dry matter content is 5.2 ~ 6.8 at 25 ~ 50g/l, pH.
5. the application of a kind of schizochytrium limacinum broken cells wall slurries as claimed in claim 1 on aquatic fish seed rearing; it is characterized in that: broken cells wall slurries add water soluble antioxidants and carry out anti-oxidation protection in storage and transport process; antioxidant be in vitamin C, sodium ascorbate, sodium isoascorbate and Tea Polyphenols any one or two kinds composite, addition 0.1 ~ 0.4%.
6. the application of a kind of schizochytrium limacinum broken cells wall slurries as claimed in claim 1 on aquatic fish seed rearing, is characterized in that: described schizochytrium limacinum broken cells wall slurry applications is in the seed rearing of grouper, large yellow croaker, mandarin fish, eel and soft-shelled turtle.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107125174A (en) * | 2017-07-17 | 2017-09-05 | 宁波大学 | A kind of method of promotion mud blood clam gonad development |
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| CN1653938A (en) * | 2005-03-09 | 2005-08-17 | 青岛森淼实业有限公司 | Mirco algal food nutrient intensifying agent prescription and its preparing process |
| CN101817738A (en) * | 2009-11-13 | 2010-09-01 | 厦门汇盛生物有限公司 | Method for extracting DHA from cells of algae and fungi by breaking cell walls |
| CN102160604A (en) * | 2011-03-01 | 2011-08-24 | 厦门汇盛生物有限公司 | Schizochytrium aggregatum powder additive and application thereof to seedling cultivation, aquiculture and culture of poultry and livestock |
| EP2505636A1 (en) * | 2011-04-01 | 2012-10-03 | Neste Oil Oyj | A method for recovering a specific cellular component from a microorganism |
| CN103156092A (en) * | 2013-03-22 | 2013-06-19 | 山东省海洋水产研究所 | Saltwater fish compound feed taking microalgae flour as fat source and processing method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107125174A (en) * | 2017-07-17 | 2017-09-05 | 宁波大学 | A kind of method of promotion mud blood clam gonad development |
| CN107125174B (en) * | 2017-07-17 | 2020-06-12 | 宁波大学 | Method for promoting gonad development of blood clam |
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Application publication date: 20151118 |