CN105052743A - 一种有效保存黄菖蒲胚性愈伤组织的方法 - Google Patents
一种有效保存黄菖蒲胚性愈伤组织的方法 Download PDFInfo
- Publication number
- CN105052743A CN105052743A CN201510514069.0A CN201510514069A CN105052743A CN 105052743 A CN105052743 A CN 105052743A CN 201510514069 A CN201510514069 A CN 201510514069A CN 105052743 A CN105052743 A CN 105052743A
- Authority
- CN
- China
- Prior art keywords
- callus
- embryo
- preservation
- medium
- capsule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 206010020649 Hyperkeratosis Diseases 0.000 title claims abstract description 140
- 238000000034 method Methods 0.000 title claims abstract description 27
- 241001633663 Iris pseudacorus Species 0.000 title claims 10
- 238000004321 preservation Methods 0.000 claims abstract description 45
- 230000000408 embryogenic effect Effects 0.000 claims abstract description 36
- 230000006698 induction Effects 0.000 claims abstract description 27
- 239000000463 material Substances 0.000 claims abstract description 9
- 238000012216 screening Methods 0.000 claims abstract description 9
- 239000002609 medium Substances 0.000 claims description 44
- 239000002775 capsule Substances 0.000 claims description 28
- 210000001161 mammalian embryo Anatomy 0.000 claims description 18
- 210000004027 cell Anatomy 0.000 claims description 16
- 241000196324 Embryophyta Species 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 13
- 229930006000 Sucrose Natural products 0.000 claims description 13
- 239000006870 ms-medium Substances 0.000 claims description 13
- 239000005720 sucrose Substances 0.000 claims description 13
- 229920001817 Agar Polymers 0.000 claims description 8
- 239000008272 agar Substances 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 8
- 230000008929 regeneration Effects 0.000 claims description 8
- 238000011069 regeneration method Methods 0.000 claims description 8
- 230000002068 genetic effect Effects 0.000 claims description 7
- 210000004209 hair Anatomy 0.000 claims description 6
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 5
- 239000003599 detergent Substances 0.000 claims description 5
- 235000013399 edible fruits Nutrition 0.000 claims description 5
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 5
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 210000002421 cell wall Anatomy 0.000 claims description 3
- 210000000805 cytoplasm Anatomy 0.000 claims description 3
- 210000003097 mucus Anatomy 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 2
- 239000012188 paraffin wax Substances 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical group C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 2
- 230000001476 alcoholic effect Effects 0.000 claims 1
- 238000001354 calcination Methods 0.000 claims 1
- 210000003855 cell nucleus Anatomy 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 230000001954 sterilising effect Effects 0.000 claims 1
- 230000004069 differentiation Effects 0.000 abstract description 11
- 241000209495 Acorus Species 0.000 abstract description 10
- 210000002257 embryonic structure Anatomy 0.000 abstract description 10
- 244000205574 Acorus calamus Species 0.000 abstract description 8
- 241000538878 Calamus calamus Species 0.000 abstract description 4
- 235000006480 Acorus calamus Nutrition 0.000 abstract description 3
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 12
- 210000001519 tissue Anatomy 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 230000009466 transformation Effects 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 235000011996 Calamus deerratus Nutrition 0.000 description 5
- 244000184734 Pyrus japonica Species 0.000 description 5
- 239000008187 granular material Substances 0.000 description 5
- 230000006872 improvement Effects 0.000 description 5
- 230000009261 transgenic effect Effects 0.000 description 5
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 5
- 229920002148 Gellan gum Polymers 0.000 description 4
- 241001180472 Iris japonica Species 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 239000008399 tap water Substances 0.000 description 4
- 235000020679 tap water Nutrition 0.000 description 4
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 3
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 3
- HFCYZXMHUIHAQI-UHFFFAOYSA-N Thidiazuron Chemical compound C=1C=CC=CC=1NC(=O)NC1=CN=NS1 HFCYZXMHUIHAQI-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000004062 cytokinin Substances 0.000 description 3
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 3
- 230000032459 dedifferentiation Effects 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 239000005648 plant growth regulator Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- ORQVWBNUTLIVLY-UHFFFAOYSA-N 6-(furan-2-ylmethyl)purin-6-amine Chemical compound N1=CN=C2N=CN=C2C1(N)CC1=CC=CO1 ORQVWBNUTLIVLY-UHFFFAOYSA-N 0.000 description 2
- 239000005972 6-Benzyladenine Substances 0.000 description 2
- CGIDKJRJBMFXKV-UHFFFAOYSA-N 6-n'-benzylpurine-6,6-diamine Chemical compound N1=CN=C2N=CN=C2C1(N)NCC1=CC=CC=C1 CGIDKJRJBMFXKV-UHFFFAOYSA-N 0.000 description 2
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 230000032823 cell division Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 2
- 229960001669 kinetin Drugs 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 238000004161 plant tissue culture Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- OVSKIKFHRZPJSS-DOMIDYPGSA-N 2-(2,4-dichlorophenoxy)acetic acid Chemical compound OC(=O)[14CH2]OC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-DOMIDYPGSA-N 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 241001113425 Iridaceae Species 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229960002523 mercuric chloride Drugs 0.000 description 1
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- -1 no browning Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510514069.0A CN105052743B (zh) | 2015-08-20 | 2015-08-20 | 一种有效保存黄菖蒲胚性愈伤组织的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510514069.0A CN105052743B (zh) | 2015-08-20 | 2015-08-20 | 一种有效保存黄菖蒲胚性愈伤组织的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105052743A true CN105052743A (zh) | 2015-11-18 |
CN105052743B CN105052743B (zh) | 2017-06-13 |
Family
ID=54482483
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510514069.0A Expired - Fee Related CN105052743B (zh) | 2015-08-20 | 2015-08-20 | 一种有效保存黄菖蒲胚性愈伤组织的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105052743B (it) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109156358A (zh) * | 2018-09-26 | 2019-01-08 | 江苏省中国科学院植物研究所 | 一种德国鸢尾幼胚诱导胚性愈伤组织的方法 |
CN111887154A (zh) * | 2020-06-12 | 2020-11-06 | 江苏省中国科学院植物研究所 | 一种德国鸢尾胚性愈伤组织低温保存的方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02163017A (ja) * | 1988-12-13 | 1990-06-22 | Shiseido Co Ltd | イリス属植物の組織培養方法 |
WO2005012507A1 (en) * | 2003-07-25 | 2005-02-10 | The University Of Melbourne | Production of plant secondary metabolites using adsorption and elicitation in cell suspension culture |
ES2304295B2 (es) * | 2006-07-31 | 2009-10-15 | Uiniversidade De Santiago De Compostela | Metodo para la micropropagacion de iris boissieri y sus aplicaciones. |
KR20090120813A (ko) * | 2008-05-20 | 2009-11-25 | 경상북도(관련부서:경상북도산림생태과학원) | 노랑무늬붓꽃의 잎 절편을 이용한 캘러스의 대량증식방법및 노랑무늬붓꽃의 대량분화방법 |
CN101816287A (zh) * | 2010-05-05 | 2010-09-01 | 南京大学 | 一种黄菖蒲愈伤组织的培养方法 |
CN101869072A (zh) * | 2009-04-24 | 2010-10-27 | 上海上房园林植物研究所 | 金心黄菖蒲的组织培养方法 |
-
2015
- 2015-08-20 CN CN201510514069.0A patent/CN105052743B/zh not_active Expired - Fee Related
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02163017A (ja) * | 1988-12-13 | 1990-06-22 | Shiseido Co Ltd | イリス属植物の組織培養方法 |
WO2005012507A1 (en) * | 2003-07-25 | 2005-02-10 | The University Of Melbourne | Production of plant secondary metabolites using adsorption and elicitation in cell suspension culture |
ES2304295B2 (es) * | 2006-07-31 | 2009-10-15 | Uiniversidade De Santiago De Compostela | Metodo para la micropropagacion de iris boissieri y sus aplicaciones. |
KR20090120813A (ko) * | 2008-05-20 | 2009-11-25 | 경상북도(관련부서:경상북도산림생태과학원) | 노랑무늬붓꽃의 잎 절편을 이용한 캘러스의 대량증식방법및 노랑무늬붓꽃의 대량분화방법 |
CN101869072A (zh) * | 2009-04-24 | 2010-10-27 | 上海上房园林植物研究所 | 金心黄菖蒲的组织培养方法 |
CN101816287A (zh) * | 2010-05-05 | 2010-09-01 | 南京大学 | 一种黄菖蒲愈伤组织的培养方法 |
Non-Patent Citations (1)
Title |
---|
吴月燕等: ""路易斯鸢尾组织培养过程中愈伤组织的诱导和芽的分化"", 《浙江农业科学》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109156358A (zh) * | 2018-09-26 | 2019-01-08 | 江苏省中国科学院植物研究所 | 一种德国鸢尾幼胚诱导胚性愈伤组织的方法 |
CN111887154A (zh) * | 2020-06-12 | 2020-11-06 | 江苏省中国科学院植物研究所 | 一种德国鸢尾胚性愈伤组织低温保存的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN105052743B (zh) | 2017-06-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102124946B (zh) | 一种芍药组织培养方法 | |
CN111937746A (zh) | 一种用于再生虎克姜花植株的系列培养试剂盒及其应用 | |
CN113317200A (zh) | 青杨雄株的组培培养基及其应用 | |
CN102613085A (zh) | 甜叶菊同源四倍体植株的培育方法 | |
CN104823844A (zh) | 一种莲属植物的组织培养方法 | |
CN109717080B (zh) | 一种提高百子莲胚性细胞继代效果的方法 | |
CN106106152B (zh) | 离体诱导百合异源四倍体的方法 | |
CN105052743B (zh) | 一种有效保存黄菖蒲胚性愈伤组织的方法 | |
CN104285791A (zh) | 一种山蜡梅组织培养与快速繁殖的方法 | |
CN104429940B (zh) | 一种获得草莓脱毒苗的方法 | |
CN104885945B (zh) | 一种香蕉化学消毒组培方法 | |
CN104996302B (zh) | 一种提高黄菖蒲愈伤组织诱导率的方法 | |
CN104115751B (zh) | 一种利用大白菜球叶获得再生植株的培养方法 | |
CN105532467A (zh) | 一种濒危羊踯躅离体组织培养繁殖与保存的方法 | |
Liu et al. | Plant regeneration from leaf explants of Rhodiola fastigiata | |
CN117084175B (zh) | 一种大理罗汉松的组培和离体保存方法 | |
CN104365474A (zh) | 一种香蕉不定芽的化学诱变方法 | |
Chen et al. | In vitro propagation of Lychnis senno Siebold et Zucc., a rare plant with potential ornamental value | |
CN102845303B (zh) | 一种扩大普陀鹅耳枥种群数量的方法 | |
CN103430851A (zh) | 一种提高黄瓜远缘杂交组培苗驯化成活率的方法 | |
CN103798139A (zh) | 一种以花瓣为外植体建立百合胚性愈伤再生体系的方法 | |
CN103798138A (zh) | 一种以雌蕊为外植体建立百合胚性愈伤再生体系的方法 | |
CN104770297B (zh) | 一种以金钱松叶片为外植体的金钱松植株再生方法 | |
CN104782481B (zh) | 借助游离小孢子培养和ems诱变创制大白菜突变体的方法 | |
CN114521489A (zh) | 一种秋水仙素变温诱导柳树染色体加倍的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170613 Termination date: 20190820 |