CN105039492A - Potato glucose culture medium and preparation method thereof - Google Patents
Potato glucose culture medium and preparation method thereof Download PDFInfo
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- CN105039492A CN105039492A CN201510479759.7A CN201510479759A CN105039492A CN 105039492 A CN105039492 A CN 105039492A CN 201510479759 A CN201510479759 A CN 201510479759A CN 105039492 A CN105039492 A CN 105039492A
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Abstract
The invention discloses a potato glucose culture medium and a preparation method thereof. The potato glucose culture medium is prepared from potatoes, glucose and agar, wherein in 100 ml of the culture medium, 15-25 g of potatoes, 1-5 g of glucose and 1.0-2.5 g of agar are contained and mixed to the rated quantity with water as a culture medium mixing agent. The preparation method includes the steps of preparing potato extractive juice and conducting subpackaging, plugging and binding. By means of the potato glucose culture medium, the yeast detection rate of the culture medium can be effectively increased; compared with a traditional method, the preparation method has the advantages that raw materials are saved, operation is easy and convenient, and mass of the potato glucose culture medium can be effectively improved.
Description
Technical field
The present invention relates to field of microbial culture technology, specifically refer to a kind of potato dextrose medium and preparation method thereof.
Background technology
PDA substratum is the abbreviation of potato dextrose medium, is a kind of semisynthetic medium of solid.And potato dextrose medium a kind of uses substratum very widely, suitable culturing yeast bacterium, mould, and the fungi such as mushroom.In national food safety standard GB4789.15-2010, potato dextrose medium can be used for detecting the yeast content in food.
The present method preparing PDA substratum is a lot, existing preparation method, and wastage of material is serious, operates comparatively loaded down with trivial details, and consuming time longer, and between the substratum of different batches, difference is larger.
Based on this, study and devise a kind of potato dextrose medium and preparation method.
Summary of the invention
The object of the invention is to: provide a kind of potato dextrose medium and preparation method, the method effectively can improve the training quality of potato dextrose medium, and improves this substratum yeast recall rate.
The present invention is achieved through the following technical solutions: provide a kind of potato dextrose medium, and in 100ml, containing potato 15-25g, glucose 1-5g, agar 1.0-2.5g, be adjusted to specified rate using water as substratum blender.
Further, in order to better realize the present invention, also comprise following technical characteristic, described substratum prepares with potato, glucose, agar, in 100ml, containing potato 20g, glucose 2g, agar 1.5-2.5g, be adjusted to specified rate using water as substratum blender.
Further, in order to better realize the present invention, also comprise following technical characteristic, in described substratum, potato is potato leaching juice.
Further, in order to better realize the present invention, also comprise following technical characteristic, described potato leaching juice is the potato 200g removing skin, be cut into small pieces, add water 1000ml, and 80 DEG C are soaked 1h, by filtered through gauze, supply dehydration to volume required, 100Pa sterilizing 20min, to obtain final product.
Present invention also offers a kind of preparation method of potato dextrose medium, comprise the following steps: (1) preparation potato leaching juice gets peeled potatoes 200g, be cut into small pieces or smash to pieces, add water 100ml, 80 DEG C are soaked 1h, filter, and then supply dehydration to volume required, 100Pa sterilizing 20-30min, to obtain final product;
(2), during preparation, add 2g glucose by every 100ml potato leaching juice, add 2g agar after heated and boiled, continue heating and melting and supply dehydration, obtaining target substratum;
(3) target substratum is carried out packing, jumps a queue, wraps up, to obtain final product.
Further, in order to better realize the present invention, also comprise following technical characteristic, filtering in described step (1) is take to be no less than two-layer hospital gauze or proprietary material filtration.
Further, in order to better realize the present invention, also comprise following technical characteristic, in described step (1) sterilising treatment be take in moist heat sterilization, high-temperature sterilization or steam instantaneous sterilizing any one.
Further, in order to better realize the present invention, also comprise following technical characteristic, in described step (1), sterilising treatment is at 121 DEG C of high-temperature sterilization 20min.
Further, in order to better realize the present invention, also comprise following technical characteristic, smashing to pieces in described step (1) is adopt stamp mill to smash 30-40s to pieces.
Further, in order to better realize the present invention, also comprise following technical characteristic, smashing to pieces in described step (1) is adopt stamp mill to smash 20-30s to pieces.
The present invention compared with prior art, has the following advantages and beneficial effect:
(1) a kind of potato dextrose medium provided by the invention, effectively can improve this substratum yeast recall rate;
(2) method preparing potato dextrose medium of the present invention, compares with traditional method, can effectively economize in raw materials, and simple to operation, and can improve the quality of potato dextrose medium.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1:
In the present embodiment, a kind of potato dextrose medium, prepares with potato, glucose, agar, and in 100ml, containing potato 20g, glucose 2g, agar 1.5g, be adjusted to specified rate using water as substratum blender.Preparation method: (1) preparation potato leaching juice, get peeled potatoes 200g, be cut into small pieces, add water 100ml, and 80 DEG C are soaked 1h, and by filtered through gauze, then supply dehydration to volume required, 100Pa sterilizing 20min, to obtain final product; (2), during preparation, add 2g glucose by every 100ml potato leaching juice, add 2g agar after heated and boiled, continue heating and melting and supply dehydration, obtaining target substratum; (3) target substratum is carried out packing, jumps a queue, wraps up, to obtain final product.
Embodiment 2:
In the present embodiment, a kind of potato dextrose medium, prepares with potato, glucose, agar, and in 100ml, containing potato 15g, glucose 1g, agar 1.0g, be adjusted to specified rate using water as substratum blender.Preparation method: (1) preparation potato leaching juice, get peeled potatoes 200g, smash 30s to pieces with stamp mill, add water 100ml, and 80 DEG C are soaked 1h, filter with two-layer hospital gauze, and then supply dehydration to volume required, 100Pa high-temperature sterilization 20min, to obtain final product; (2), during preparation, add 2g glucose by every 100ml potato leaching juice, add 2g agar after heated and boiled, continue heating and melting and supply dehydration, obtaining target substratum; (3) target substratum is carried out packing, jumps a queue, wraps up, to obtain final product.
Embodiment 3:
In the present embodiment, a kind of potato dextrose medium, prepares with potato, glucose, agar, and in 100ml, containing potato 25g, glucose 5g, agar 2.5g, be adjusted to specified rate using water as substratum blender.Preparation method: (1) preparation potato leaching juice gets peeled potatoes 200g, and smash 20s to pieces with stamp mill, add water 100ml, and 80 DEG C are soaked 1h, filter with two-layer hospital gauze, and then supply dehydration to volume required, 100Pa moist heat sterilization 20min, to obtain final product; (2), during preparation, add 2g glucose by every 100ml potato leaching juice, add 2g agar after heated and boiled, continue heating and melting and supply dehydration, obtaining target substratum; (3) target substratum is carried out packing, jumps a queue, wraps up, to obtain final product.
Embodiment 4:
In the present embodiment, a kind of potato dextrose medium, prepares with potato, glucose, agar, and in 100ml, containing potato 20g, glucose 2g, agar 2g, be adjusted to specified rate using water as substratum blender.Preparation method: (1) preparation potato leaching juice gets peeled potatoes 200g, and smash 30s to pieces with stamp mill, add water 100ml, and 80 DEG C are soaked 1h, filter with two-layer hospital gauze, and then supply dehydration to volume required, 100Pa moist heat sterilization 20min, to obtain final product; (2), during preparation, add 2g glucose by every 100ml potato leaching juice, add 2g agar after heated and boiled, continue heating and melting and supply dehydration, obtaining target substratum; (3) target substratum is carried out packing, jumps a queue, wraps up, to obtain final product.
The above is only preferred embodiment of the present invention, and not do any pro forma restriction to the present invention, every any simple modification, equivalent variations done above embodiment according to technical spirit of the present invention, all falls within protection scope of the present invention.
Claims (10)
1. a potato dextrose medium, is characterized in that, in 100ml, containing potato 15-25g, glucose 1-5g, agar 1.0-2.5g, be adjusted to specified rate using water as substratum blender.
2. a kind of potato dextrose medium according to claim 1, is characterized in that, in 100ml, containing potato 20g, glucose 2g, agar 1.5-2.5g, be adjusted to specified rate using water as substratum blender.
3. a kind of potato dextrose medium according to claim 2, is characterized in that, in described substratum, potato is potato leaching juice.
4. a kind of potato dextrose medium according to claim 3, it is characterized in that, the preparation method of described potato leaching juice is: the potato 200g removing skin, be cut into small pieces, add water 1000ml, and 80 DEG C are soaked 1h, by filtered through gauze, supply dehydration to volume required, 100Pa sterilizing 20min, to obtain final product.
5. the preparation method of a potato dextrose medium, it is characterized in that: comprise the following steps: (1) preparation potato leaching juice, get peeled potatoes 200g, be cut into small pieces or smash to pieces, add water 100ml, and 80 DEG C are soaked 1h, filter, then supply dehydration to volume required, 100Pa sterilizing 20-30min, to obtain final product;
(2), during preparation, add 2g glucose by every 100ml potato leaching juice, add 2g agar after heated and boiled, continue heating and melting and supply dehydration, obtaining target substratum;
(3) target substratum is carried out packing, jumps a queue, wraps up, to obtain final product.
6. the preparation method of a kind of potato dextrose medium according to claim 5, is characterized in that: filtering in described step (1) is take to be no less than two-layer hospital gauze or proprietary material filtration.
7. the preparation method of a kind of potato dextrose medium according to claim 6, is characterized in that: in described step (1), sterilising treatment takes any one in moist heat sterilization, high-temperature sterilization or steam instantaneous sterilizing.
8. the preparation method of a kind of potato dextrose medium according to claim 7, is characterized in that: in described step (1), sterilising treatment is at 121 DEG C of high-temperature sterilization 20min.
9. the preparation method of a kind of potato dextrose medium according to claim 8, is characterized in that: smashing to pieces in described step (1) is adopt stamp mill to smash 30-40s to pieces.
10. the preparation method of a kind of potato dextrose medium according to claim 8, is characterized in that: smashing to pieces in described step (1) is adopt stamp mill to smash 20-30s to pieces.
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| CN201510479759.7A CN105039492A (en) | 2015-08-07 | 2015-08-07 | Potato glucose culture medium and preparation method thereof |
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| CN201510479759.7A CN105039492A (en) | 2015-08-07 | 2015-08-07 | Potato glucose culture medium and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105325295A (en) * | 2015-11-06 | 2016-02-17 | 贵州腾博农业发展有限公司 | Potato culture medium and preparing method thereof |
| CN105925485A (en) * | 2016-07-08 | 2016-09-07 | 黑龙江省科学院大庆分院 | Improved culture medium for soybean phytophthora preservation |
| CN110511859A (en) * | 2019-08-22 | 2019-11-29 | 广西农业职业技术学院 | A kind of equipment and working method preparing potato culture |
-
2015
- 2015-08-07 CN CN201510479759.7A patent/CN105039492A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 丁浩等: "马铃薯葡萄糖培养制作方法的改进", 《中国酿造》 * |
| 武汉理工大学出版社: "《微生物基础技术》", 31 October 2010 * |
| 章家恩主编: "《普通生态学实验指导》", 31 August 2012 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105325295A (en) * | 2015-11-06 | 2016-02-17 | 贵州腾博农业发展有限公司 | Potato culture medium and preparing method thereof |
| CN105925485A (en) * | 2016-07-08 | 2016-09-07 | 黑龙江省科学院大庆分院 | Improved culture medium for soybean phytophthora preservation |
| CN110511859A (en) * | 2019-08-22 | 2019-11-29 | 广西农业职业技术学院 | A kind of equipment and working method preparing potato culture |
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Application publication date: 20151111 Assignee: SICHAUN DICHEN AGRICULTURAL LIMITED LIABILITY COMPANY Assignor: Yi Chuansi bio tech ltd, Chengdu Contract record no.: 2016510000008 Denomination of invention: Potato glucose culture medium and preparation method thereof License type: Common License Record date: 20160506 |
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Application publication date: 20151111 |
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