CN105039485B - A method of improving turmeric saponin extract yield - Google Patents
A method of improving turmeric saponin extract yield Download PDFInfo
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- CN105039485B CN105039485B CN201510375903.2A CN201510375903A CN105039485B CN 105039485 B CN105039485 B CN 105039485B CN 201510375903 A CN201510375903 A CN 201510375903A CN 105039485 B CN105039485 B CN 105039485B
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- turmeric saponin
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Abstract
The present invention provides a kind of method for improving turmeric saponin extract yield.The following steps are included: crushed material first is made by after the cleaning removal of impurities of cadmium yellow ginger；Then single Discrete control temperature is used, the method of method and two-stage temperature control combination pH synergic adjustment that constant pH is 5~6 pre-processes crushed material 24 hours, the crushed material after fermentation is transferred in hydrolysis bottle again and carries out sour water solution, the hydrolysate isolated is adjusted to neutrality, just extract is made in hydrolysate drying；Turmeric saponin is obtained after extract is dried.Method and process process provided in the present invention is simple, low energy consumption, and the yield of turmeric saponin improves 30% or more compared with the yield of turmeric saponin in existing pre fermentation technique.
Description
Technical field
The present invention provides a kind of methods for producing turmeric saponin, particularly provide a kind of raising turmeric saponin extract yield
New method, belong to technical field of biochemical industry.
Background technique
Yellow ginger also known as dioscorea zingiberensis wright, be Dioscoreaceae Dioscorea (Dioscrea.L) herbaceos perennial is potato in the world
Highest kind of Chinese yam sapogenin content.Sapogenin is the important foundation raw material for producing steroid hormone class drug.Steroid hormone has anti-
Infection, antiallergy, antiviral and Antishock function.
Currently, more in relation to improving the processing method report of yield in the production technology of turmeric saponin.For example, Tong Ling etc.
Yellow ginger is handled using enzymatic hydrolysis pre fermentation, the final saponin yield of turmeric saponin is average up to 2.37 %, with obtaining for direct acid-hydrolysis method
1.84 % of rate, which compares the raising for having by a relatively large margin, can be improved that (enzymatic isolation methods such as 20030601 Tong Ling of Shaanxi Normal University's journal mention
Take the technical study of Chinese yam saponin).A kind of domestic applications patent " cleaning manufacturing technique method of turmeric saponin " (application number
A kind of method for 201310144472.X) describing direct pre fermentation processing, 45-60 DEG C of spontaneous fermentation 48-72h processing, brown windsor soap
Plain yield also significantly improves；In addition, to also indicate that the yield of saponin increases (high for some thermal extractions and high pressure homogenization method technique
School chemical engineering journal, the research of the turmeric saponins novel technology for extracting such as 20110402 Pan He woodss).Although the above report method
All yield of higher amplitude raising turmeric saponin.However, the yield of turmeric saponin still has very big room for promotion, in order to more
It is significant to improve yield.The present invention significantly improves pre fermentation effect to temperature and pH value synergic adjustment by a large number of experiments early period
Fruit, to efficiently obtain turmeric saponin.
Summary of the invention
The purpose of the present invention is to provide a kind of processes for improving turmeric saponin extract yield.The process is root
According in yellow ginger cell the characteristics of various endogenous enzymes, in the pre fermentation stage that turmeric saponin extracts using temperature section control, in conjunction with
The method of pH synergic adjustment guarantees that various endogenous enzymes are had an effect, and gives full play to the effect of various endogenous enzymes during the fermentation,
Achieve the purpose that improve turmeric saponin extract yield, reduces the yellow ginger consumption of unit saponin product, reduce the discharge of waste, mention
High efficiency reduces production cost.
In order to achieve the above purpose, the present invention is finally obtained as follows once by a large number of experiments research and persistent exploration
Technical solution:
A method of improving turmeric saponin extract yield, it is characterised in that: the following steps are included:
(1) yellow ginger is cleaned and is crushed, crushed material is made；
(2) Discrete control temperature fermentation, constant pH 5~6 are used, it is characterised in that: temperature section controls 42 DEG C of fermentations 6
Hour, it then heats to 55 DEG C and ferments 12 hours, then be warming up to 68 DEG C and ferment 6 hours, the pre fermentation time is 24 hours；
(3) crushed material after fermentation is transferred in hydrolysis bottle and carries out sour water solution, hydrolysate is separated；
(4) hydrolysate isolated is adjusted to neutrality, then dries hydrolysate, just extract is made；
(5) primary extract is extracted using gasoline or petroleum ether, obtains turmeric saponin after extract is dried.
Preferably, a kind of method improving turmeric saponin extract yield as described above, it is characterised in that: including following step
It is rapid:
(1) yellow ginger is cleaned and is crushed, crushed material is made；
(2) it is fermented using sectional temperature-controlled combination pH synergic adjustment, it is characterised in that: when pre fermentation starts, temperature 42
DEG C, pH4.5~5.5 ferment 6 hours, then heat to 55 DEG C, pH3.5~4.5 ferment 12 hours, then be warming up to 68 DEG C, pH3.5
~3.0 fermentations 6 hours, pre fermentation time are 24 hours；
(3) crushed material after fermentation is transferred in hydrolysis bottle and carries out sour water solution, hydrolysate is separated；
(4) hydrolysate isolated is adjusted to neutrality, then dries hydrolysate, just extract is made；
(5) primary extract is extracted using gasoline or petroleum ether, obtains turmeric saponin after extract is dried.
Preferably, a kind of method improving turmeric saponin extract yield as described above, it is characterised in that: yellow ginger pretreatment
Method be by cadmium yellow ginger cleaning removal of impurities after, in the mass ratio of cadmium yellow ginger and water be 1:2~3 ratio add pigment to be broken to partial size 2-
5mm。
Compared with prior art, the technology of the present invention has verified single Discrete control temperature, method that constant pH is 5~6 and
Influence of the method for two-stage temperature control combination pH synergic adjustment to turmeric saponin extract yield, it is determined that two-stage temperature control
In conjunction with the method for pH synergic adjustment.
Method and process process provided in the present invention is simple, low energy consumption, the yield of turmeric saponin and existing pre fermentation
The yield of turmeric saponin is compared in technique, improves 30% or more.
Specific embodiment
The following is specific embodiments of the present invention, is further described to technical solution of the present invention, but of the invention
Protection scope is not limited to these examples.It is all to be included in the present invention without departing substantially from the change of present inventive concept or equivalent substitute
Within protection scope.
Embodiment 1
Firstly, being inquired into the preprocess method of cadmium yellow ginger, process program are as follows: after the cleaning removal of impurities of cadmium yellow ginger,
Pigment are added to be broken to partial size 2-5mm in the ratio that the mass ratio of cadmium yellow ginger and water is 1:2~3.
Secondly, single Discrete control temperature has been inquired into, method and two-stage temperature control combination the pH association of constant pH 5~6
Influence with the method adjusted to turmeric saponin extract yield, the specific method is as follows:
4200 grams of cadmium yellow ginger are taken, are the ratio of 1:2~3 in the mass ratio of cadmium yellow ginger and water after the cleaning removal of impurities of cadmium yellow ginger
Add pigment to be broken to partial size 2-5mm, it is uniformly divided into 21 equal portions；Three groups of tests are carried out, every group test seven parallel.It is each parallel
Crushed material is taken out after the completion of test pre fermentation, the crushed material after fermentation is transferred in hydrolysis bottle and carries out sour water solution, by hydrolysate point
From；The hydrolysate isolated is adjusted to neutrality, then dries hydrolysate, primary extract is made；Using gasoline or petroleum ether pair
Primary extract extracts, and obtains turmeric saponin after extract is dried.The technical method of the extract yield of turmeric saponin is pair
The turmeric saponin yield of seven parallel tests removes its peak and maximum value, and remaining five are averaged in parallel.
According to above-mentioned specific embodiment, testing program is as follows with result:
Test group one as a control group (number be test No. 1), experimental condition are as follows: under conditions of 70 DEG C, 5~6 pH
Pre fermentation 24 hours.
Test group two is testing program one (number is test 2), is single Discrete control temperature, constant pH 5~6.
Experimental condition are as follows: 42 DEG C ferment 6 hours, then heat to 55 DEG C and ferment 12 hours, then are warming up to 68 DEG C and ferment 6 hours, and pH is permanent
It is set to 5~6.
Test group three is testing program two (number is test 3), is two-stage temperature control combination pH synergic adjustment.Examination
Test condition are as follows: 42 DEG C, the fermentation of pH4.5~5.5 6 hours then heat to 55 DEG C, the fermentation of pH3.5~4.5 12 hours, then heat up
It ferments 6 hours to 68 DEG C, pH3.5~3.0.
Through overtesting and calculating, the yield of turmeric saponin the results are shown in Table 1 in each group.
Influence of the 1 pre fermentation mode of table to turmeric saponin yield
From upper table it is known that using single Discrete control temperature, constant pH is 5~6 24 hours sides of pre fermentation
15% or so is improved within pre fermentation 24 hours under conditions of method, the final yield of turmeric saponin and existing 70 DEG C, pH 5~6；
Using two-stage temperature control combination pH synergic adjustment 24 hours methods of pre fermentation, the final yield of turmeric saponin with it is existing
70 DEG C, improve 30% or more within pre fermentation 24 hours under conditions of pH 5~6.
Claims (3)
1. a kind of method for improving turmeric saponin extract yield, it is characterised in that: the following steps are included:
(1) yellow ginger is cleaned and is crushed, crushed material is made；
(2) Discrete control temperature fermentation, constant pH 5~6 are used, it is characterised in that: temperature section controls 42 DEG C and ferments 6 hours,
It then heats to 55 DEG C to ferment 12 hours, then is warming up to 68 DEG C and ferments 6 hours, the pre fermentation time is 24 hours；
(3) crushed material after fermentation is transferred in hydrolysis bottle and carries out sour water solution, hydrolysate is separated；
(4) hydrolysate isolated is adjusted to neutrality, then dries hydrolysate, just extract is made；
(5) primary extract is extracted using gasoline or petroleum ether, obtains turmeric saponin after extract is dried.
2. a kind of method for improving turmeric saponin extract yield as described in claim 1, it is characterised in that: including following step
It is rapid:
(1) yellow ginger is cleaned and is crushed, crushed material is made；
(2) using sectional temperature-controlled combination pH synergic adjustment ferment, it is characterised in that: when pre fermentation starts, temperature be 42 DEG C,
PH4.5~5.5 ferment 6 hours, then heat to 55 DEG C, pH3.5~4.5 ferment 12 hours, then be warming up to 68 DEG C, pH3.5~
3.0 fermentations 6 hours, pre fermentation time are 24 hours；
(3) crushed material after fermentation is transferred in hydrolysis bottle and carries out sour water solution, hydrolysate is separated；
(4) hydrolysate isolated is adjusted to neutrality, then dries hydrolysate, just extract is made；
(5) primary extract is extracted using gasoline or petroleum ether, obtains turmeric saponin after extract is dried.
3. a kind of method for improving turmeric saponin extract yield of any of claims 1 or 2, it is characterised in that: yellow ginger pretreatment
Method be by cadmium yellow ginger cleaning removal of impurities after, in the mass ratio of cadmium yellow ginger and water be 1:2~3 ratio add pigment to be broken to partial size 2-
5mm。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510375903.2A CN105039485B (en) | 2015-07-01 | 2015-07-01 | A method of improving turmeric saponin extract yield |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510375903.2A CN105039485B (en) | 2015-07-01 | 2015-07-01 | A method of improving turmeric saponin extract yield |
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| CN105039485A CN105039485A (en) | 2015-11-11 |
| CN105039485B true CN105039485B (en) | 2019-04-05 |
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| CN110904181A (en) * | 2019-11-20 | 2020-03-24 | 华南农业大学 | Method for improving saponin yield of Dioscorea composita by natural fermentation |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560239A (en) * | 2009-05-25 | 2009-10-21 | 北京大学 | Production process for extracting saponins from yellow ginger by using solvent catalyzing method |
| CN101575358A (en) * | 2009-05-25 | 2009-11-11 | 北京大学 | Method for extracting saponin from hydrolysate with high saponin content |
| CN102464898A (en) * | 2010-11-13 | 2012-05-23 | 陕西理工学院 | Method for recovering yellow ginger pigment from yellow ginger hydrolysate saponin extraction waste liquid |
| CN103059095A (en) * | 2013-01-07 | 2013-04-24 | 李玉山 | Green extraction technology for comprehensive and efficient utilization of Dioscorea zingiberensis resource |
| CN103882087A (en) * | 2014-04-22 | 2014-06-25 | 中国地质大学（武汉） | Rapid fermentation production method of yam diosgenin |
-
2015
- 2015-07-01 CN CN201510375903.2A patent/CN105039485B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560239A (en) * | 2009-05-25 | 2009-10-21 | 北京大学 | Production process for extracting saponins from yellow ginger by using solvent catalyzing method |
| CN101575358A (en) * | 2009-05-25 | 2009-11-11 | 北京大学 | Method for extracting saponin from hydrolysate with high saponin content |
| CN102464898A (en) * | 2010-11-13 | 2012-05-23 | 陕西理工学院 | Method for recovering yellow ginger pigment from yellow ginger hydrolysate saponin extraction waste liquid |
| CN103059095A (en) * | 2013-01-07 | 2013-04-24 | 李玉山 | Green extraction technology for comprehensive and efficient utilization of Dioscorea zingiberensis resource |
| CN103882087A (en) * | 2014-04-22 | 2014-06-25 | 中国地质大学（武汉） | Rapid fermentation production method of yam diosgenin |
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